dntp mixture (TaKaRa)
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TaKaRa
dntp mixture
Dntp Mixture, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 8908 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dntp/dNTP+Mixture/pm42884072-243-15-20
Average 96 stars, based on 8908 article reviews
Dntp Mixture, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 8908 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dntp/dNTP+Mixture/pm42884072-243-15-20
Average 96 stars, based on 8908 article reviews
dntp mixture - by Bioz Stars,
2026-10
96/100 stars
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Polymerase Chain Reaction:Article Title: Digital analyte analysis Article Snippet: Approximately 25 μL of the PCR reaction mixture was collected as an emulsion from the droplet generation chip and thermally cycled in a DNA Engine (Bio-Rad, Hercules, CA). .. The reaction mixture contained 1× TaqMan® universal PCR master mix (Applied Biosystems, Carlsbad, CA), 0.2 mM Article Title: Digital analyte analysis Article Snippet: Approximately 25 μL of the PCR reaction mixture was collected as an emulsion from the droplet generation chip and thermally cycled in a DNA Engine (Bio-Rad, Hercules, CA). .. The reaction mixture contained 1× TaqMan® universal PCR master mix (Applied Biosystems, Carlsbad, CA), 0.2 mM Article Title: The association between ADIPOQ gene variants (rs266729, rs2241766, rs1501299) and acute myocardial infarction in Vietnamese patients with type 2 diabetes mellitus Article Snippet: .. The PCR reactions were prepared with 50 ng of DNA and a final master mix for each reaction containing 1X PCR Buffer (#R007A; Takara Bio, Kusatsu, Shiga, Japan), 200 μM Concentration Assay:Article Title: BADLOCK: A Rapid, Portable, Inexpensive Diagnostic for Bacterial Pathogen and Resistance Detection in Resource-Limited Settings Article Snippet: .. To do so, a single master mix was made combining the following reagents: 13uL of the diluted and lysed bacterial template, 99ul TwistAmp® liquid basic 2x Buffer (TwistDxTM) (0.8x final concentration), 13.2ul TwistAmp® liquid basic 20x Core Reaction Mix (1x final concentration), 26.4ul TwistAmp® liquid basic 10x E- mix (1x final concentration), 26.4ul of a Article Title: Identification of potential predators of western bean cutworm (<i>Striacosta albicosta</i>) in field corn through molecular gut‐content analysis Article Snippet: .. Each aliquot consisted of 17.375 μL ddH20; 2.5 μL 10× EX Taq buffer with 20 mM MgCl2 (Takara Bio Inc., Japan); 2 μL cDNA Library Assay:Article Title: A Versatile, Simple, and Rapid (VSR) method for generating gene clones, diverse mutants, and short gene fragments for molecular biology applications Article Snippet: RNA samples from various mammalian cell lines, including human embryonic kidney 293T cells (HEK293T-ATCC CRL-3216, Manassas, VA, USA), the human liver cell line, and the human lung cell line (Huh7 and A549 (Erasmus Medical Centre, Rotterdam, Netherlands)), were isolated using the RNeasy Mini Kit (Qiagen, #74104) as per the manufacturer’s instructions. .. To synthesize the cDNA library, 0.5‒1 μg of the isolated RNA was added to the reaction mix containing 0.5 μl (40 U/μl) of RNase inhibitor (Promega, #N2618), 1 μl (2.5 mM each) of Isolation:Article Title: A Versatile, Simple, and Rapid (VSR) method for generating gene clones, diverse mutants, and short gene fragments for molecular biology applications Article Snippet: RNA samples from various mammalian cell lines, including human embryonic kidney 293T cells (HEK293T-ATCC CRL-3216, Manassas, VA, USA), the human liver cell line, and the human lung cell line (Huh7 and A549 (Erasmus Medical Centre, Rotterdam, Netherlands)), were isolated using the RNeasy Mini Kit (Qiagen, #74104) as per the manufacturer’s instructions. .. To synthesize the cDNA library, 0.5‒1 μg of the isolated RNA was added to the reaction mix containing 0.5 μl (40 U/μl) of RNase inhibitor (Promega, #N2618), 1 μl (2.5 mM each) of Random Hexamer:Article Title: A Versatile, Simple, and Rapid (VSR) method for generating gene clones, diverse mutants, and short gene fragments for molecular biology applications Article Snippet: RNA samples from various mammalian cell lines, including human embryonic kidney 293T cells (HEK293T-ATCC CRL-3216, Manassas, VA, USA), the human liver cell line, and the human lung cell line (Huh7 and A549 (Erasmus Medical Centre, Rotterdam, Netherlands)), were isolated using the RNeasy Mini Kit (Qiagen, #74104) as per the manufacturer’s instructions. .. To synthesize the cDNA library, 0.5‒1 μg of the isolated RNA was added to the reaction mix containing 0.5 μl (40 U/μl) of RNase inhibitor (Promega, #N2618), 1 μl (2.5 mM each) of other:Article Title: Protocol to clone unknown flanking genomic region using SWPOP-PCR for genome walking Article Snippet: dNTP (dATP, dTTP, dCTP, and Ligation:Article Title: abcFISH enables multiplexed, single-molecule visualization of circular RNA spatial heterogeneity Article Snippet: After hybridization, samples were washed twice with PBSTR (0.1% Tween-20, 0.2 U/μL RNasin in RNase-free 1×PBS) at 37 °C for 20 min each time, followed by washing with 4× SSC buffer in PBSTR once at 37 °C for 20 min. After another quick wash with PBSTR, the ligase reaction mixture (0.25 U/μL T4 DNA ligase (Thermo Scientific, EL0012), 0.5 mg/ml BSA (Sangon, A000290), and 0.4 U/μL of RNasin in 1× T4 DNA ligase buffer) was then added to the sample for a 2 h incubation at 25 °C. .. Following ligation, samples were washed with PBSTR twice and incubated with RCA reaction solution (0.5 U/μL Phi29 DNA polymerase (Vazyme, N106-02), 250 μM Incubation:Article Title: abcFISH enables multiplexed, single-molecule visualization of circular RNA spatial heterogeneity Article Snippet: After hybridization, samples were washed twice with PBSTR (0.1% Tween-20, 0.2 U/μL RNasin in RNase-free 1×PBS) at 37 °C for 20 min each time, followed by washing with 4× SSC buffer in PBSTR once at 37 °C for 20 min. After another quick wash with PBSTR, the ligase reaction mixture (0.25 U/μL T4 DNA ligase (Thermo Scientific, EL0012), 0.5 mg/ml BSA (Sangon, A000290), and 0.4 U/μL of RNasin in 1× T4 DNA ligase buffer) was then added to the sample for a 2 h incubation at 25 °C. .. Following ligation, samples were washed with PBSTR twice and incubated with RCA reaction solution (0.5 U/μL Phi29 DNA polymerase (Vazyme, N106-02), 250 μM Hybridization:Article Title: abcFISH enables multiplexed, single-molecule visualization of circular RNA spatial heterogeneity Article Snippet: After hybridization, samples were washed twice with PBSTR (0.1% Tween-20, 0.2 U/μL RNasin in RNase-free 1×PBS) at 37 °C for 20 min each time, followed by washing with 4× SSC buffer in PBSTR once at 37 °C for 20 min. After another quick wash with PBSTR, the ligase reaction mixture (0.25 U/μL T4 DNA ligase (Thermo Scientific, EL0012), 0.5 mg/ml BSA (Sangon, A000290), and 0.4 U/μL of RNasin in 1× T4 DNA ligase buffer) was then added to the sample for a 2 h incubation at 25 °C. .. Following ligation, samples were washed with PBSTR twice and incubated with RCA reaction solution (0.5 U/μL Phi29 DNA polymerase (Vazyme, N106-02), 250 μM |