dntp mixture  (TaKaRa)

 
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    Name:
    PrimeSTAR Max DNA Polymerase
    Description:
    PrimeSTAR Max DNA Polymerase has the highest fidelity and fastest extension rate of any Takara Bio enzyme The formulation is based on Takara s unique high fidelity PrimeSTAR HS DNA Polymerase combined with an elongation factor to provide efficient priming and extension which greatly reduces the time required for annealing and extension steps As a result PrimeSTAR Max DNA Polymerase can be used for exceptionally fast PCR It is formulated as a 2X premix containing optimized buffer and dNTPs which allows for quick preparation of reactions for high throughput applications
    Catalog Number:
    r045b
    Price:
    None
    Size:
    400 Rxns
    Category:
    PrimeSTAR Max DNA Polymerase High fidelity PCR PCR
    Buy from Supplier


    Structured Review

    TaKaRa dntp mixture
    PrimeSTAR Max DNA Polymerase has the highest fidelity and fastest extension rate of any Takara Bio enzyme The formulation is based on Takara s unique high fidelity PrimeSTAR HS DNA Polymerase combined with an elongation factor to provide efficient priming and extension which greatly reduces the time required for annealing and extension steps As a result PrimeSTAR Max DNA Polymerase can be used for exceptionally fast PCR It is formulated as a 2X premix containing optimized buffer and dNTPs which allows for quick preparation of reactions for high throughput applications
    https://www.bioz.com/result/dntp mixture/product/TaKaRa
    Average 90 stars, based on 77 article reviews
    Price from $9.99 to $1999.99
    dntp mixture - by Bioz Stars, 2020-01
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Micromanaging Iron Homeostasis
    Article Snippet: The PCR products were cloned into a pGEM-T Easy Vector (Promega; Madison, WI). .. PrimeStar Max DNA Polymerase (Takara; Kyoto, Japan) was used for PCR amplification.

    Article Title: Structural Basis of Pharmacological Chaperoning for Human β-Galactosidase *
    Article Snippet: Paragraph title: Cloning, Expression, and Purification of Human β-Gal ... Mutations were introduced by PrimeSTAR Max DNA polymerase (Takara) using oligonucleotide primers following the manufacturer's protocol.

    Amplification:

    Article Title: Dengue Virus Infection in Aedes albopictus during the 2014 Autochthonous Dengue Outbreak in Tokyo Metropolis, Japan
    Article Snippet: .. The cDNAs were amplified by a PCR using PrimeSTAR Max DNA polymerase (Takara Bio Inc.). .. Primer sets for cDNA amplification were as follows: DENV1_F1, 5′-CGG AAG CTT GCT TAA CGT AG-3; DENV1_R2, 5′-GCC CTA CAG CAA ACA TTG GT-3′; DENV1_F3.2, 5′-GCT GAT GAC TGG AAC ACT GG-3′; DENV1_R4.2, 5′-CCG TTG TGT TTT CGA TTG TG-3′; DENV1_F5.2, 5′-TTC AGC CTG GAC CCT TTA TG-3′; and DENV1_R6.2, 5′-CAG CCT CCC AGG TTT TTA CA-3′.

    Article Title: Micromanaging Iron Homeostasis
    Article Snippet: .. PrimeStar Max DNA Polymerase (Takara; Kyoto, Japan) was used for PCR amplification. ..

    Article Title: A novel function of N-linked glycoproteins, alpha-2-HS-glycoprotein and hemopexin: Implications for small molecule compound-mediated neuroprotection
    Article Snippet: .. For the synthesis of glutathione S-transferase (GST) fusion proteins by the wheat germ cell-free system, three human cDNAs, AHSG (FXC03806), SERPINA1 (FXC03481) and RBP4 (FXC03871), were obtained from Flexi ORF clone (Promega, Madison, WI) and were amplified by PCR in a GeneAmp PCR system 9700 (Applied Biosystems, Tokyo) with PrimeSTAR Max DNA Polymerase (TaKaRa) according to the manufacturer's instructions. ..

    Article Title: Distinct interactions of EBP1 isoforms with FBXW7 elicits different functions in cancer
    Article Snippet: The shRNA sequences for FBXW7 are as follows: sh FBXW7 A: GGCAACAACGACGCCGAAT; sh FBXW7 B: GAGTTGGCACTCTATGTGC.EBP1 P48 and P42 human cDNA were subcloned using the PrimeSTAR Max DNA polymerase (TaKaRa, Japan) into the Myc-CMV10 vector and Flag-CMV10 vector. .. Ubiquitin gene was amplified and subcloned into Myc-CMV10 vector and pCGN-HA vector.

    Article Title: ALDH enzyme activity is regulated by Nodal and histamine in the A549 cell line
    Article Snippet: .. The resulting cDNA was amplified in a final volume of 25 µl containing 2.5 pmol of each primer and 12.5 µl of PrimeSTAR MAX DNA polymerase (Takara Bio, Inc., Otsu, Japan). .. The amplification conditions were denaturation at 98°C for 10 sec (5 min for the first cycle), annealing at 55°C for 5 sec, and extension at 72°C for 1 min for 40 cycles for the amplification of H1R, H2R and H4R.

    DNA Synthesis:

    Article Title: Effects of combined radiosurgery and temozolomide therapy on epidermal growth factor receptor and variant III in glioblastoma multiforme
    Article Snippet: The GoScript™ Reverse Transcriptase system (Promega Corporation, Madison, WI, USA) was used in complementary DNA synthesis according to the manufacturer's instructions. .. All PCR was performed using a PCR kit (PrimeSTAR® Max DNA Polymerase; cat. no. R045A; Takara Biotechnology Co., Ltd., Dalian, China), according to the manufacturer's instructions.

    Synthesized:

    Article Title: Dengue Virus Infection in Aedes albopictus during the 2014 Autochthonous Dengue Outbreak in Tokyo Metropolis, Japan
    Article Snippet: For the analysis of whole coding sequences, viral cDNAs were synthesized using the ThermoScript RT-PCR System for First-Strand cDNA Synthesis (Thermo Fisher Scientific, Waltham, MA) from RNA extracted from the supernatant of the Vero cells. .. The cDNAs were amplified by a PCR using PrimeSTAR Max DNA polymerase (Takara Bio Inc.).

    Article Title: Extracellular translationally controlled tumor protein promotes colorectal cancer invasion and metastasis through Cdc42/JNK/ MMP9 signaling
    Article Snippet: DNA Polymerase (Cat: R045A), T4 DNA ligase (Cat: 2011A), restriction enzymes (Eco R I, Cat: 1040S; No t I, Cat: 1166S) and competent cells (Cat: 9057) were purchased from Takara (Dalian, China). .. Small interfering RNAs (siRNA) were synthesized by Genepharma (Suzhou, China).

    TA Cloning:

    Article Title: Phosphorylation of Farnesoid X Receptor at Serine 154 Links Ligand Activation With Degradation
    Article Snippet: An antibody against GFP (632381) was obtained from Clontech Laboratories, Inc, and antibodies against VDR (sc-1009), retinoid X receptor (RXR)α (sc-553), retinoic acid receptor-related orphan receptor (ROR)α (sc-6062), ERα (sc-7207), β-actin (sc-47778), ubiquitin (sc-9133), and TBP (sc-273) were from Santa Cruz Biotechnology, Inc. Antibody to V5 (46-0705), TOPO TA cloning kit with pCR2.1 TOPO, pcDNA3.1/V5-His TOPO TA Expression kit, Dynabeads Protein G, and Lipofectamine 2000 were from Invitrogen. .. PrimeSTAR Max DNA Polymerase and LA-taq DNA polymerase were obtained from Takara.

    Construct:

    Article Title: Micromanaging Iron Homeostasis
    Article Snippet: Paragraph title: 3′-UTR Assay Plasmid Constructs ... PrimeStar Max DNA Polymerase (Takara; Kyoto, Japan) was used for PCR amplification.

    Article Title: Distinct interactions of EBP1 isoforms with FBXW7 elicits different functions in cancer
    Article Snippet: Wild type HA-FBXW7α, FBXW7α mutants (ΔWD, ΔF,), sh FBXW7 A and sh FBXW7 B plasmids were previously constructed by our lab ( ) ( , , ). .. The shRNA sequences for FBXW7 are as follows: sh FBXW7 A: GGCAACAACGACGCCGAAT; sh FBXW7 B: GAGTTGGCACTCTATGTGC.EBP1 P48 and P42 human cDNA were subcloned using the PrimeSTAR Max DNA polymerase (TaKaRa, Japan) into the Myc-CMV10 vector and Flag-CMV10 vector.

    SYBR Green Assay:

    Article Title: Spatial transcriptomic survey of human embryonic cerebral cortex by single-cell RNA-seq analysis
    Article Snippet: Then reverse transcription was carried out in a volume of 19 μl, containing 2.5 ng/μl oligod(T) primer (Takara, Cat# 3806), 0.5 mM dNTP mixture (Takara, Cat# R045B), 2 U/μl RNase inhibitor (Ambion, Cat# AM2684), 5 mM DTT, 1× first strand and 0.5 U/μl superscript III reverse transcriptase (Invitrogen, Cat# 18080-044). .. The qPCR detection was run on CFX connect system (Biorad) using SYBR Green (Roche, Cat# 13396700).

    Luciferase:

    Article Title: Micromanaging Iron Homeostasis
    Article Snippet: The amplified products were ligated into the XhoI and NotI sites of the 3′-UTR of the Renilla luciferase gene in the psi-check-2 plasmid (Promega) to generate psi-ISCU and psi-TfR. .. PrimeStar Max DNA Polymerase (Takara; Kyoto, Japan) was used for PCR amplification.

    Expressing:

    Article Title: Effects of combined radiosurgery and temozolomide therapy on epidermal growth factor receptor and variant III in glioblastoma multiforme
    Article Snippet: The expression levels of mature EGFRvIII were determined by semi-quantitative PCR using a PCR cycler (Nanjing KeyGenBiotech, Co., Ltd., Beijing, China) with a forward primer for the mature DNA sequence and a universal adaptor reverse primer as follows: Forward, 5′-TGACTCCGTCCAGTATTGATCG-3′ and reverse, 5′-GCCCTTCGCACTTCTTACACTT-3′ (Thermo Fisher Scientific Inc.). .. All PCR was performed using a PCR kit (PrimeSTAR® Max DNA Polymerase; cat. no. R045A; Takara Biotechnology Co., Ltd., Dalian, China), according to the manufacturer's instructions.

    Article Title: Distinct interactions of EBP1 isoforms with FBXW7 elicits different functions in cancer
    Article Snippet: Paragraph title: Expression plasmids ... The shRNA sequences for FBXW7 are as follows: sh FBXW7 A: GGCAACAACGACGCCGAAT; sh FBXW7 B: GAGTTGGCACTCTATGTGC.EBP1 P48 and P42 human cDNA were subcloned using the PrimeSTAR Max DNA polymerase (TaKaRa, Japan) into the Myc-CMV10 vector and Flag-CMV10 vector.

    Article Title: Structural Basis of Pharmacological Chaperoning for Human β-Galactosidase *
    Article Snippet: Paragraph title: Cloning, Expression, and Purification of Human β-Gal ... Mutations were introduced by PrimeSTAR Max DNA polymerase (Takara) using oligonucleotide primers following the manufacturer's protocol.

    Article Title: Phosphorylation of Farnesoid X Receptor at Serine 154 Links Ligand Activation With Degradation
    Article Snippet: An antibody against GFP (632381) was obtained from Clontech Laboratories, Inc, and antibodies against VDR (sc-1009), retinoid X receptor (RXR)α (sc-553), retinoic acid receptor-related orphan receptor (ROR)α (sc-6062), ERα (sc-7207), β-actin (sc-47778), ubiquitin (sc-9133), and TBP (sc-273) were from Santa Cruz Biotechnology, Inc. Antibody to V5 (46-0705), TOPO TA cloning kit with pCR2.1 TOPO, pcDNA3.1/V5-His TOPO TA Expression kit, Dynabeads Protein G, and Lipofectamine 2000 were from Invitrogen. .. PrimeSTAR Max DNA Polymerase and LA-taq DNA polymerase were obtained from Takara.

    BIA-KA:

    Article Title: Analysis of redox landscapes and dynamics in living cells andin vivo using genetically encoded fluorescent sensors
    Article Snippet: .. 60210ES25) Isopropyl-β-D-thiogalactoside (Yeasen, cat. no. 10902ES08) Tryptone (Oxoid, cat. no.LP0042) Yeast extract (Oxoid, cat. no.LP0021) Ampicillin (Yeasen, cat. no. 60203ES10) Na3 PO4 (Sigma, cat. no. 342483) NaCl (Sigma, cat. no. 746398) Imidazole (Sigma, cat. no. 576166) HEPES (Vetec, cat. no. V900477) KCl (Shanghai Titan Scientific Co., Ltd., cat. no. G80636B) KH2 PO4 (Vetec, cat. no. V900041) Na2 HPO4 · 12 H2 O (Shanghai Titan Scientific Co., Ltd., cat. no. G10267A) MgSO4 (Vetec, cat. no. V900066) CaCl2 (Vetec, cat. no. V900266) NaHCO3 (Vetec, cat. no. V900182) PrimeSTAR MAX DNA polymerase (Takara, cat. no. R045A) SP6 Primer (Generay) SV40 Poly(A) Primer (Generay) BSA, bovine serum albumin (Yeasen, cat. no. 36101ES60) BCA protein quantification kit (Yeasen, cat. no. 20201ES76) SanPrep column DNA gel extraction kit (Sangon Biotech, cat. no. B518131) mMESSAGE mMACHINE kit (Invitrogen, cat. no. AM1340) Agarose (Yeason, cat. no. 10208ES60) NADPH (Yeason, cat. no. 60302ES01) IFN-γ, recombinant mouse interferon γ (Absin, cat. no. abs01021d) LPS, lipopolysaccharides from Escherichia coli (Yeasen, cat. no. 60322ES10) Tricaine (Sigma, cat. no. A5040). ..

    Modification:

    Article Title: Extracellular translationally controlled tumor protein promotes colorectal cancer invasion and metastasis through Cdc42/JNK/ MMP9 signaling
    Article Snippet: Dulbecco's Modified Eagle Medium (DMEM, Cat: 11965118) was from Life Technologies (Grand Island, NY, USA). .. DNA Polymerase (Cat: R045A), T4 DNA ligase (Cat: 2011A), restriction enzymes (Eco R I, Cat: 1040S; No t I, Cat: 1166S) and competent cells (Cat: 9057) were purchased from Takara (Dalian, China).

    Crystallization Assay:

    Article Title: Structural Basis of Pharmacological Chaperoning for Human β-Galactosidase *
    Article Snippet: Mutations were introduced by PrimeSTAR Max DNA polymerase (Takara) using oligonucleotide primers following the manufacturer's protocol. .. For crystallization, β-Gals were subjected to limited proteolysis with bovine trypsin and further purified by cation-exchange column chromatography, and Superdex 200 size exclusion column (GE Healthcare) equilibrated with buffer A (0.01 m MES, pH 6.0, 0.1 m NaCl).

    Activated Clotting Time Assay:

    Article Title: Dengue Virus Infection in Aedes albopictus during the 2014 Autochthonous Dengue Outbreak in Tokyo Metropolis, Japan
    Article Snippet: The cDNAs were amplified by a PCR using PrimeSTAR Max DNA polymerase (Takara Bio Inc.). .. Primer sets for cDNA amplification were as follows: DENV1_F1, 5′-CGG AAG CTT GCT TAA CGT AG-3; DENV1_R2, 5′-GCC CTA CAG CAA ACA TTG GT-3′; DENV1_F3.2, 5′-GCT GAT GAC TGG AAC ACT GG-3′; DENV1_R4.2, 5′-CCG TTG TGT TTT CGA TTG TG-3′; DENV1_F5.2, 5′-TTC AGC CTG GAC CCT TTA TG-3′; and DENV1_R6.2, 5′-CAG CCT CCC AGG TTT TTA CA-3′.

    Countercurrent Chromatography:

    Article Title: Dengue Virus Infection in Aedes albopictus during the 2014 Autochthonous Dengue Outbreak in Tokyo Metropolis, Japan
    Article Snippet: The cDNAs were amplified by a PCR using PrimeSTAR Max DNA polymerase (Takara Bio Inc.). .. Primer sets for cDNA amplification were as follows: DENV1_F1, 5′-CGG AAG CTT GCT TAA CGT AG-3; DENV1_R2, 5′-GCC CTA CAG CAA ACA TTG GT-3′; DENV1_F3.2, 5′-GCT GAT GAC TGG AAC ACT GG-3′; DENV1_R4.2, 5′-CCG TTG TGT TTT CGA TTG TG-3′; DENV1_F5.2, 5′-TTC AGC CTG GAC CCT TTA TG-3′; and DENV1_R6.2, 5′-CAG CCT CCC AGG TTT TTA CA-3′.

    Transfection:

    Article Title: Analysis of redox landscapes and dynamics in living cells andin vivo using genetically encoded fluorescent sensors
    Article Snippet: JM109 (DE3) chemically competent cells (Yeasen, cat. no. 11807ES80) DMEM (HyClone, cat. no. SH30243.01) RPMI 1640 medium (HyClone, cat. no. SH30809.01B) Fetal bovine serum (Gibco, cat. no. 10270-106) Phenol red-free DMEM (Gibco, cat. no. 21063029) Penicillin-streptomycin solution (HyClone, cat. no. SV30010) 0.25% (wt/vol) trypsin-EDTA (Gibco, cat. no. 25200-072) Hieff Trans liposomal transfection reagent (Yeasen, cat. no. 40802ES03) FuGENE HD transfection reagent (Promega, cat. no. E2311) Opti-MEM I reduced-serum medium (Invitrogen, cat. no. 31985-070) DPBS (HyClone, cat. no. SH30013.04) D-glucose (Sigma, cat. no. G7021) Poly-brene (Sigma, cat.no. .. 60210ES25) Isopropyl-β-D-thiogalactoside (Yeasen, cat. no. 10902ES08) Tryptone (Oxoid, cat. no.LP0042) Yeast extract (Oxoid, cat. no.LP0021) Ampicillin (Yeasen, cat. no. 60203ES10) Na3 PO4 (Sigma, cat. no. 342483) NaCl (Sigma, cat. no. 746398) Imidazole (Sigma, cat. no. 576166) HEPES (Vetec, cat. no. V900477) KCl (Shanghai Titan Scientific Co., Ltd., cat. no. G80636B) KH2 PO4 (Vetec, cat. no. V900041) Na2 HPO4 · 12 H2 O (Shanghai Titan Scientific Co., Ltd., cat. no. G10267A) MgSO4 (Vetec, cat. no. V900066) CaCl2 (Vetec, cat. no. V900266) NaHCO3 (Vetec, cat. no. V900182) PrimeSTAR MAX DNA polymerase (Takara, cat. no. R045A) SP6 Primer (Generay) SV40 Poly(A) Primer (Generay) BSA, bovine serum albumin (Yeasen, cat. no. 36101ES60) BCA protein quantification kit (Yeasen, cat. no. 20201ES76) SanPrep column DNA gel extraction kit (Sangon Biotech, cat. no. B518131) mMESSAGE mMACHINE kit (Invitrogen, cat. no. AM1340) Agarose (Yeason, cat. no. 10208ES60) NADPH (Yeason, cat. no. 60302ES01) IFN-γ, recombinant mouse interferon γ (Absin, cat. no. abs01021d) LPS, lipopolysaccharides from Escherichia coli (Yeasen, cat. no. 60322ES10) Tricaine (Sigma, cat. no. A5040).

    Concentration Assay:

    Article Title: Effects of combined radiosurgery and temozolomide therapy on epidermal growth factor receptor and variant III in glioblastoma multiforme
    Article Snippet: The concentration and purity of RNA were determined using a NanoDrop® ND-1000 spectrophotometer (Thermo Fisher Scientific, Inc.). .. All PCR was performed using a PCR kit (PrimeSTAR® Max DNA Polymerase; cat. no. R045A; Takara Biotechnology Co., Ltd., Dalian, China), according to the manufacturer's instructions.

    Cell Culture:

    Article Title: Extracellular translationally controlled tumor protein promotes colorectal cancer invasion and metastasis through Cdc42/JNK/ MMP9 signaling
    Article Snippet: Paragraph title: Cell culture and chemicals ... DNA Polymerase (Cat: R045A), T4 DNA ligase (Cat: 2011A), restriction enzymes (Eco R I, Cat: 1040S; No t I, Cat: 1166S) and competent cells (Cat: 9057) were purchased from Takara (Dalian, China).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Effects of combined radiosurgery and temozolomide therapy on epidermal growth factor receptor and variant III in glioblastoma multiforme
    Article Snippet: Paragraph title: RNA extraction and polymerase chain reaction (RT-PCR) ... All PCR was performed using a PCR kit (PrimeSTAR® Max DNA Polymerase; cat. no. R045A; Takara Biotechnology Co., Ltd., Dalian, China), according to the manufacturer's instructions.

    Article Title: Dengue Virus Infection in Aedes albopictus during the 2014 Autochthonous Dengue Outbreak in Tokyo Metropolis, Japan
    Article Snippet: For the analysis of whole coding sequences, viral cDNAs were synthesized using the ThermoScript RT-PCR System for First-Strand cDNA Synthesis (Thermo Fisher Scientific, Waltham, MA) from RNA extracted from the supernatant of the Vero cells. .. The cDNAs were amplified by a PCR using PrimeSTAR Max DNA polymerase (Takara Bio Inc.).

    Article Title: A novel function of N-linked glycoproteins, alpha-2-HS-glycoprotein and hemopexin: Implications for small molecule compound-mediated neuroprotection
    Article Snippet: For the synthesis of glutathione S-transferase (GST) fusion proteins by the wheat germ cell-free system, three human cDNAs, AHSG (FXC03806), SERPINA1 (FXC03481) and RBP4 (FXC03871), were obtained from Flexi ORF clone (Promega, Madison, WI) and were amplified by PCR in a GeneAmp PCR system 9700 (Applied Biosystems, Tokyo) with PrimeSTAR Max DNA Polymerase (TaKaRa) according to the manufacturer's instructions. .. Four human cDNAs, A1BG , CFB , ITIH4 and HPX were amplified from Human Total RNA Master Panel II (Clontech, Palo Alto, CA) by RT-PCR with iScript cDNA synthesis kit (Bio-Rad) and PrimeSTAR Max DNA Polymerase (TaKaRa), following the manufacturer's instructions.

    Article Title: ALDH enzyme activity is regulated by Nodal and histamine in the A549 cell line
    Article Snippet: Paragraph title: Reverse transcription-polymerase chain reaction ... The resulting cDNA was amplified in a final volume of 25 µl containing 2.5 pmol of each primer and 12.5 µl of PrimeSTAR MAX DNA polymerase (Takara Bio, Inc., Otsu, Japan).

    Generated:

    Article Title: Distinct interactions of EBP1 isoforms with FBXW7 elicits different functions in cancer
    Article Snippet: The shRNA sequences for FBXW7 are as follows: sh FBXW7 A: GGCAACAACGACGCCGAAT; sh FBXW7 B: GAGTTGGCACTCTATGTGC.EBP1 P48 and P42 human cDNA were subcloned using the PrimeSTAR Max DNA polymerase (TaKaRa, Japan) into the Myc-CMV10 vector and Flag-CMV10 vector. .. Flag-EBP1 P48 S40A, Flag-EBP1 P48 S44A, HA-FBXW7α mutants (R465C, R465H, R479H, R505C) and HA-FBXW7αΔFR465C were generated using PCR-directed mutagenesis method.

    Article Title: Phosphorylation of Farnesoid X Receptor at Serine 154 Links Ligand Activation With Degradation
    Article Snippet: Anti-P-S154 peptide antibody against human FXR was generated by GeneScript. .. PrimeSTAR Max DNA Polymerase and LA-taq DNA polymerase were obtained from Takara.

    other:

    Article Title: LplR, a Repressor Belonging to the TetR Family, Regulates Expression of the l-Pantoyl Lactone Dehydrogenase Gene in Rhodococcus erythropolis
    Article Snippet: Restriction enzymes, Ex Taq DNA polymerase, and PrimeStar Max DNA polymerase were purchased from TaKaRa-Bio (Japan).

    Polymerase Chain Reaction:

    Article Title: Effects of combined radiosurgery and temozolomide therapy on epidermal growth factor receptor and variant III in glioblastoma multiforme
    Article Snippet: .. All PCR was performed using a PCR kit (PrimeSTAR® Max DNA Polymerase; cat. no. R045A; Takara Biotechnology Co., Ltd., Dalian, China), according to the manufacturer's instructions. .. The PCR cycling conditions were as follows: 1 cycle at 95°C for 10 min; 35 cycles at 95°C for 30 sec, 58°C for 30 sec and 72°C for 1 min; and 1 cycle at 72°C for 10 min.

    Article Title: Dengue Virus Infection in Aedes albopictus during the 2014 Autochthonous Dengue Outbreak in Tokyo Metropolis, Japan
    Article Snippet: .. The cDNAs were amplified by a PCR using PrimeSTAR Max DNA polymerase (Takara Bio Inc.). .. Primer sets for cDNA amplification were as follows: DENV1_F1, 5′-CGG AAG CTT GCT TAA CGT AG-3; DENV1_R2, 5′-GCC CTA CAG CAA ACA TTG GT-3′; DENV1_F3.2, 5′-GCT GAT GAC TGG AAC ACT GG-3′; DENV1_R4.2, 5′-CCG TTG TGT TTT CGA TTG TG-3′; DENV1_F5.2, 5′-TTC AGC CTG GAC CCT TTA TG-3′; and DENV1_R6.2, 5′-CAG CCT CCC AGG TTT TTA CA-3′.

    Article Title: Micromanaging Iron Homeostasis
    Article Snippet: .. PrimeStar Max DNA Polymerase (Takara; Kyoto, Japan) was used for PCR amplification. ..

    Article Title: A novel function of N-linked glycoproteins, alpha-2-HS-glycoprotein and hemopexin: Implications for small molecule compound-mediated neuroprotection
    Article Snippet: .. For the synthesis of glutathione S-transferase (GST) fusion proteins by the wheat germ cell-free system, three human cDNAs, AHSG (FXC03806), SERPINA1 (FXC03481) and RBP4 (FXC03871), were obtained from Flexi ORF clone (Promega, Madison, WI) and were amplified by PCR in a GeneAmp PCR system 9700 (Applied Biosystems, Tokyo) with PrimeSTAR Max DNA Polymerase (TaKaRa) according to the manufacturer's instructions. ..

    Article Title: Distinct interactions of EBP1 isoforms with FBXW7 elicits different functions in cancer
    Article Snippet: The shRNA sequences for FBXW7 are as follows: sh FBXW7 A: GGCAACAACGACGCCGAAT; sh FBXW7 B: GAGTTGGCACTCTATGTGC.EBP1 P48 and P42 human cDNA were subcloned using the PrimeSTAR Max DNA polymerase (TaKaRa, Japan) into the Myc-CMV10 vector and Flag-CMV10 vector. .. Flag-EBP1 P48 S40A, Flag-EBP1 P48 S44A, HA-FBXW7α mutants (R465C, R465H, R479H, R505C) and HA-FBXW7αΔFR465C were generated using PCR-directed mutagenesis method.

    Article Title: Biosynthesis and genetic encoding of phosphothreonine through parallel selection and deep sequencing
    Article Snippet: To derive PylS library 1 ( M. barkeri PylS residues A267, Y271, L274, and C313 randomised to NNK), position M241 was fixed by EI-PCR using PrimeStar Max DNA polymerase (Clontech) with the following primer pair: 5’-GCGCAGGTCTCAGAACGTNDTGGCATTAACAACGACACCGAACTGAGCAAA C-3' (F) and 5’-gcgcaGAGTAGGTCTCAGTTCCACATATTCCGCCGGAATCAGAATC-3' (R). .. The PCR products were gel extracted, digested by BsaI, re-circularized by T4 DNA ligase, and electroporated into DH10B competent cells to create a library with a diversity of ≈109 colonly forming units (c.f.u), surpassing the required 107 c.f.u.

    Recombinant:

    Article Title: Analysis of redox landscapes and dynamics in living cells andin vivo using genetically encoded fluorescent sensors
    Article Snippet: .. 60210ES25) Isopropyl-β-D-thiogalactoside (Yeasen, cat. no. 10902ES08) Tryptone (Oxoid, cat. no.LP0042) Yeast extract (Oxoid, cat. no.LP0021) Ampicillin (Yeasen, cat. no. 60203ES10) Na3 PO4 (Sigma, cat. no. 342483) NaCl (Sigma, cat. no. 746398) Imidazole (Sigma, cat. no. 576166) HEPES (Vetec, cat. no. V900477) KCl (Shanghai Titan Scientific Co., Ltd., cat. no. G80636B) KH2 PO4 (Vetec, cat. no. V900041) Na2 HPO4 · 12 H2 O (Shanghai Titan Scientific Co., Ltd., cat. no. G10267A) MgSO4 (Vetec, cat. no. V900066) CaCl2 (Vetec, cat. no. V900266) NaHCO3 (Vetec, cat. no. V900182) PrimeSTAR MAX DNA polymerase (Takara, cat. no. R045A) SP6 Primer (Generay) SV40 Poly(A) Primer (Generay) BSA, bovine serum albumin (Yeasen, cat. no. 36101ES60) BCA protein quantification kit (Yeasen, cat. no. 20201ES76) SanPrep column DNA gel extraction kit (Sangon Biotech, cat. no. B518131) mMESSAGE mMACHINE kit (Invitrogen, cat. no. AM1340) Agarose (Yeason, cat. no. 10208ES60) NADPH (Yeason, cat. no. 60302ES01) IFN-γ, recombinant mouse interferon γ (Absin, cat. no. abs01021d) LPS, lipopolysaccharides from Escherichia coli (Yeasen, cat. no. 60322ES10) Tricaine (Sigma, cat. no. A5040). ..

    Cellular Antioxidant Activity Assay:

    Article Title: Dengue Virus Infection in Aedes albopictus during the 2014 Autochthonous Dengue Outbreak in Tokyo Metropolis, Japan
    Article Snippet: The cDNAs were amplified by a PCR using PrimeSTAR Max DNA polymerase (Takara Bio Inc.). .. Primer sets for cDNA amplification were as follows: DENV1_F1, 5′-CGG AAG CTT GCT TAA CGT AG-3; DENV1_R2, 5′-GCC CTA CAG CAA ACA TTG GT-3′; DENV1_F3.2, 5′-GCT GAT GAC TGG AAC ACT GG-3′; DENV1_R4.2, 5′-CCG TTG TGT TTT CGA TTG TG-3′; DENV1_F5.2, 5′-TTC AGC CTG GAC CCT TTA TG-3′; and DENV1_R6.2, 5′-CAG CCT CCC AGG TTT TTA CA-3′.

    Mutagenesis:

    Article Title: Micromanaging Iron Homeostasis
    Article Snippet: Site-directed mutagenesis or deletion mutant was performed in the seed sequences of ISCU and TfR. .. PrimeStar Max DNA Polymerase (Takara; Kyoto, Japan) was used for PCR amplification.

    Article Title: Distinct interactions of EBP1 isoforms with FBXW7 elicits different functions in cancer
    Article Snippet: The shRNA sequences for FBXW7 are as follows: sh FBXW7 A: GGCAACAACGACGCCGAAT; sh FBXW7 B: GAGTTGGCACTCTATGTGC.EBP1 P48 and P42 human cDNA were subcloned using the PrimeSTAR Max DNA polymerase (TaKaRa, Japan) into the Myc-CMV10 vector and Flag-CMV10 vector. .. Flag-EBP1 P48 S40A, Flag-EBP1 P48 S44A, HA-FBXW7α mutants (R465C, R465H, R479H, R505C) and HA-FBXW7αΔFR465C were generated using PCR-directed mutagenesis method.

    Article Title: Structural Basis of Pharmacological Chaperoning for Human β-Galactosidase *
    Article Snippet: Mutations were introduced by PrimeSTAR Max DNA polymerase (Takara) using oligonucleotide primers following the manufacturer's protocol. .. Briefly, β-GalWT and two mutant proteins, β-GalR201C and β-GalI51T (residues 24–677), fused to the N-terminal hexahistidine and FLAG tag, were expressed in yeast Pichia pastoris KM71 and purified to homogeneity in a nickel-Sepharose column.

    Size-exclusion Chromatography:

    Article Title: Effects of combined radiosurgery and temozolomide therapy on epidermal growth factor receptor and variant III in glioblastoma multiforme
    Article Snippet: All PCR was performed using a PCR kit (PrimeSTAR® Max DNA Polymerase; cat. no. R045A; Takara Biotechnology Co., Ltd., Dalian, China), according to the manufacturer's instructions. .. The PCR cycling conditions were as follows: 1 cycle at 95°C for 10 min; 35 cycles at 95°C for 30 sec, 58°C for 30 sec and 72°C for 1 min; and 1 cycle at 72°C for 10 min.

    Article Title: ALDH enzyme activity is regulated by Nodal and histamine in the A549 cell line
    Article Snippet: The resulting cDNA was amplified in a final volume of 25 µl containing 2.5 pmol of each primer and 12.5 µl of PrimeSTAR MAX DNA polymerase (Takara Bio, Inc., Otsu, Japan). .. The amplification conditions were denaturation at 98°C for 10 sec (5 min for the first cycle), annealing at 55°C for 5 sec, and extension at 72°C for 1 min for 40 cycles for the amplification of H1R, H2R and H4R.

    Purification:

    Article Title: Dengue Virus Infection in Aedes albopictus during the 2014 Autochthonous Dengue Outbreak in Tokyo Metropolis, Japan
    Article Snippet: The amplicons were purified by the AMPure XP kit (Beckman Coulter, Brea, CA) and sequenced using the ABI PRISM BigDye Terminator Cycle Sequencing Kit Ver. .. The cDNAs were amplified by a PCR using PrimeSTAR Max DNA polymerase (Takara Bio Inc.).

    Article Title: Structural Basis of Pharmacological Chaperoning for Human β-Galactosidase *
    Article Snippet: Paragraph title: Cloning, Expression, and Purification of Human β-Gal ... Mutations were introduced by PrimeSTAR Max DNA polymerase (Takara) using oligonucleotide primers following the manufacturer's protocol.

    Sequencing:

    Article Title: Effects of combined radiosurgery and temozolomide therapy on epidermal growth factor receptor and variant III in glioblastoma multiforme
    Article Snippet: The expression levels of mature EGFRvIII were determined by semi-quantitative PCR using a PCR cycler (Nanjing KeyGenBiotech, Co., Ltd., Beijing, China) with a forward primer for the mature DNA sequence and a universal adaptor reverse primer as follows: Forward, 5′-TGACTCCGTCCAGTATTGATCG-3′ and reverse, 5′-GCCCTTCGCACTTCTTACACTT-3′ (Thermo Fisher Scientific Inc.). .. All PCR was performed using a PCR kit (PrimeSTAR® Max DNA Polymerase; cat. no. R045A; Takara Biotechnology Co., Ltd., Dalian, China), according to the manufacturer's instructions.

    Article Title: Dengue Virus Infection in Aedes albopictus during the 2014 Autochthonous Dengue Outbreak in Tokyo Metropolis, Japan
    Article Snippet: Paragraph title: Analysis of the viral genome sequence. ... The cDNAs were amplified by a PCR using PrimeSTAR Max DNA polymerase (Takara Bio Inc.).

    Quantitative RT-PCR:

    Article Title: Spatial transcriptomic survey of human embryonic cerebral cortex by single-cell RNA-seq analysis
    Article Snippet: Paragraph title: RT-qPCR validation ... Then reverse transcription was carried out in a volume of 19 μl, containing 2.5 ng/μl oligod(T) primer (Takara, Cat# 3806), 0.5 mM dNTP mixture (Takara, Cat# R045B), 2 U/μl RNase inhibitor (Ambion, Cat# AM2684), 5 mM DTT, 1× first strand and 0.5 U/μl superscript III reverse transcriptase (Invitrogen, Cat# 18080-044).

    Gel Extraction:

    Article Title: Analysis of redox landscapes and dynamics in living cells andin vivo using genetically encoded fluorescent sensors
    Article Snippet: .. 60210ES25) Isopropyl-β-D-thiogalactoside (Yeasen, cat. no. 10902ES08) Tryptone (Oxoid, cat. no.LP0042) Yeast extract (Oxoid, cat. no.LP0021) Ampicillin (Yeasen, cat. no. 60203ES10) Na3 PO4 (Sigma, cat. no. 342483) NaCl (Sigma, cat. no. 746398) Imidazole (Sigma, cat. no. 576166) HEPES (Vetec, cat. no. V900477) KCl (Shanghai Titan Scientific Co., Ltd., cat. no. G80636B) KH2 PO4 (Vetec, cat. no. V900041) Na2 HPO4 · 12 H2 O (Shanghai Titan Scientific Co., Ltd., cat. no. G10267A) MgSO4 (Vetec, cat. no. V900066) CaCl2 (Vetec, cat. no. V900266) NaHCO3 (Vetec, cat. no. V900182) PrimeSTAR MAX DNA polymerase (Takara, cat. no. R045A) SP6 Primer (Generay) SV40 Poly(A) Primer (Generay) BSA, bovine serum albumin (Yeasen, cat. no. 36101ES60) BCA protein quantification kit (Yeasen, cat. no. 20201ES76) SanPrep column DNA gel extraction kit (Sangon Biotech, cat. no. B518131) mMESSAGE mMACHINE kit (Invitrogen, cat. no. AM1340) Agarose (Yeason, cat. no. 10208ES60) NADPH (Yeason, cat. no. 60302ES01) IFN-γ, recombinant mouse interferon γ (Absin, cat. no. abs01021d) LPS, lipopolysaccharides from Escherichia coli (Yeasen, cat. no. 60322ES10) Tricaine (Sigma, cat. no. A5040). ..

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Analysis of redox landscapes and dynamics in living cells andin vivo using genetically encoded fluorescent sensors
    Article Snippet: 40119ES10) Puromycin (Yeasen, cat.no. .. 60210ES25) Isopropyl-β-D-thiogalactoside (Yeasen, cat. no. 10902ES08) Tryptone (Oxoid, cat. no.LP0042) Yeast extract (Oxoid, cat. no.LP0021) Ampicillin (Yeasen, cat. no. 60203ES10) Na3 PO4 (Sigma, cat. no. 342483) NaCl (Sigma, cat. no. 746398) Imidazole (Sigma, cat. no. 576166) HEPES (Vetec, cat. no. V900477) KCl (Shanghai Titan Scientific Co., Ltd., cat. no. G80636B) KH2 PO4 (Vetec, cat. no. V900041) Na2 HPO4 · 12 H2 O (Shanghai Titan Scientific Co., Ltd., cat. no. G10267A) MgSO4 (Vetec, cat. no. V900066) CaCl2 (Vetec, cat. no. V900266) NaHCO3 (Vetec, cat. no. V900182) PrimeSTAR MAX DNA polymerase (Takara, cat. no. R045A) SP6 Primer (Generay) SV40 Poly(A) Primer (Generay) BSA, bovine serum albumin (Yeasen, cat. no. 36101ES60) BCA protein quantification kit (Yeasen, cat. no. 20201ES76) SanPrep column DNA gel extraction kit (Sangon Biotech, cat. no. B518131) mMESSAGE mMACHINE kit (Invitrogen, cat. no. AM1340) Agarose (Yeason, cat. no. 10208ES60) NADPH (Yeason, cat. no. 60302ES01) IFN-γ, recombinant mouse interferon γ (Absin, cat. no. abs01021d) LPS, lipopolysaccharides from Escherichia coli (Yeasen, cat. no. 60322ES10) Tricaine (Sigma, cat. no. A5040).

    Article Title: Extracellular translationally controlled tumor protein promotes colorectal cancer invasion and metastasis through Cdc42/JNK/ MMP9 signaling
    Article Snippet: Phospho-SAPK/JNK (Thr183/Tyr185) Rabbit mAb (Cat: 4668P), Cdc42 Antibody (Cat: 2462), Akt antibody (Cat:9272), phospho-Akt (Ser473) antibody (Cat: 4060), c-Jun Mouse mAb (Cat: 2315), phospho-c-jun (Ser63) antibody (Cat: 2361), MMP-2 Rabbit mAb (Cat: 4022), MMP-9 Rabbit mAb (Cat: 13667) were purchased from Cell Signaling Technology (Boston, MA, USA). .. DNA Polymerase (Cat: R045A), T4 DNA ligase (Cat: 2011A), restriction enzymes (Eco R I, Cat: 1040S; No t I, Cat: 1166S) and competent cells (Cat: 9057) were purchased from Takara (Dalian, China).

    Plasmid Preparation:

    Article Title: Micromanaging Iron Homeostasis
    Article Snippet: Paragraph title: 3′-UTR Assay Plasmid Constructs ... PrimeStar Max DNA Polymerase (Takara; Kyoto, Japan) was used for PCR amplification.

    Article Title: A novel function of N-linked glycoproteins, alpha-2-HS-glycoprotein and hemopexin: Implications for small molecule compound-mediated neuroprotection
    Article Snippet: Paragraph title: Plasmid construction ... For the synthesis of glutathione S-transferase (GST) fusion proteins by the wheat germ cell-free system, three human cDNAs, AHSG (FXC03806), SERPINA1 (FXC03481) and RBP4 (FXC03871), were obtained from Flexi ORF clone (Promega, Madison, WI) and were amplified by PCR in a GeneAmp PCR system 9700 (Applied Biosystems, Tokyo) with PrimeSTAR Max DNA Polymerase (TaKaRa) according to the manufacturer's instructions.

    Article Title: Distinct interactions of EBP1 isoforms with FBXW7 elicits different functions in cancer
    Article Snippet: .. The shRNA sequences for FBXW7 are as follows: sh FBXW7 A: GGCAACAACGACGCCGAAT; sh FBXW7 B: GAGTTGGCACTCTATGTGC.EBP1 P48 and P42 human cDNA were subcloned using the PrimeSTAR Max DNA polymerase (TaKaRa, Japan) into the Myc-CMV10 vector and Flag-CMV10 vector. .. FBXW7αNΔF were subcloned into the pCGN-HA vector.

    Software:

    Article Title: Dengue Virus Infection in Aedes albopictus during the 2014 Autochthonous Dengue Outbreak in Tokyo Metropolis, Japan
    Article Snippet: The cDNAs were amplified by a PCR using PrimeSTAR Max DNA polymerase (Takara Bio Inc.). .. The sequencing data were assembled using CLC Genomic Workbench Software (QIAGEN).

    Real-time Polymerase Chain Reaction:

    Article Title: Spatial transcriptomic survey of human embryonic cerebral cortex by single-cell RNA-seq analysis
    Article Snippet: Then reverse transcription was carried out in a volume of 19 μl, containing 2.5 ng/μl oligod(T) primer (Takara, Cat# 3806), 0.5 mM dNTP mixture (Takara, Cat# R045B), 2 U/μl RNase inhibitor (Ambion, Cat# AM2684), 5 mM DTT, 1× first strand and 0.5 U/μl superscript III reverse transcriptase (Invitrogen, Cat# 18080-044). .. The qPCR detection was run on CFX connect system (Biorad) using SYBR Green (Roche, Cat# 13396700).

    RNA Extraction:

    Article Title: Effects of combined radiosurgery and temozolomide therapy on epidermal growth factor receptor and variant III in glioblastoma multiforme
    Article Snippet: Paragraph title: RNA extraction and polymerase chain reaction (RT-PCR) ... All PCR was performed using a PCR kit (PrimeSTAR® Max DNA Polymerase; cat. no. R045A; Takara Biotechnology Co., Ltd., Dalian, China), according to the manufacturer's instructions.

    shRNA:

    Article Title: Distinct interactions of EBP1 isoforms with FBXW7 elicits different functions in cancer
    Article Snippet: .. The shRNA sequences for FBXW7 are as follows: sh FBXW7 A: GGCAACAACGACGCCGAAT; sh FBXW7 B: GAGTTGGCACTCTATGTGC.EBP1 P48 and P42 human cDNA were subcloned using the PrimeSTAR Max DNA polymerase (TaKaRa, Japan) into the Myc-CMV10 vector and Flag-CMV10 vector. .. FBXW7αNΔF were subcloned into the pCGN-HA vector.

    Column Chromatography:

    Article Title: Structural Basis of Pharmacological Chaperoning for Human β-Galactosidase *
    Article Snippet: Mutations were introduced by PrimeSTAR Max DNA polymerase (Takara) using oligonucleotide primers following the manufacturer's protocol. .. For crystallization, β-Gals were subjected to limited proteolysis with bovine trypsin and further purified by cation-exchange column chromatography, and Superdex 200 size exclusion column (GE Healthcare) equilibrated with buffer A (0.01 m MES, pH 6.0, 0.1 m NaCl).

    Spectrophotometry:

    Article Title: Effects of combined radiosurgery and temozolomide therapy on epidermal growth factor receptor and variant III in glioblastoma multiforme
    Article Snippet: The concentration and purity of RNA were determined using a NanoDrop® ND-1000 spectrophotometer (Thermo Fisher Scientific, Inc.). .. All PCR was performed using a PCR kit (PrimeSTAR® Max DNA Polymerase; cat. no. R045A; Takara Biotechnology Co., Ltd., Dalian, China), according to the manufacturer's instructions.

    FLAG-tag:

    Article Title: Structural Basis of Pharmacological Chaperoning for Human β-Galactosidase *
    Article Snippet: Mutations were introduced by PrimeSTAR Max DNA polymerase (Takara) using oligonucleotide primers following the manufacturer's protocol. .. Briefly, β-GalWT and two mutant proteins, β-GalR201C and β-GalI51T (residues 24–677), fused to the N-terminal hexahistidine and FLAG tag, were expressed in yeast Pichia pastoris KM71 and purified to homogeneity in a nickel-Sepharose column.

    CTG Assay:

    Article Title: Dengue Virus Infection in Aedes albopictus during the 2014 Autochthonous Dengue Outbreak in Tokyo Metropolis, Japan
    Article Snippet: The cDNAs were amplified by a PCR using PrimeSTAR Max DNA polymerase (Takara Bio Inc.). .. Primer sets for cDNA amplification were as follows: DENV1_F1, 5′-CGG AAG CTT GCT TAA CGT AG-3; DENV1_R2, 5′-GCC CTA CAG CAA ACA TTG GT-3′; DENV1_F3.2, 5′-GCT GAT GAC TGG AAC ACT GG-3′; DENV1_R4.2, 5′-CCG TTG TGT TTT CGA TTG TG-3′; DENV1_F5.2, 5′-TTC AGC CTG GAC CCT TTA TG-3′; and DENV1_R6.2, 5′-CAG CCT CCC AGG TTT TTA CA-3′.

    Staining:

    Article Title: ALDH enzyme activity is regulated by Nodal and histamine in the A549 cell line
    Article Snippet: The resulting cDNA was amplified in a final volume of 25 µl containing 2.5 pmol of each primer and 12.5 µl of PrimeSTAR MAX DNA polymerase (Takara Bio, Inc., Otsu, Japan). .. The amplification products (each 10 µl) were separated on 2% agarose and stained by ethidium bromide.

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    TaKaRa dntp mixture
    Dntp Mixture, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa takara dntp mixture
    Takara Dntp Mixture, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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