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GenBiotech dntp
Dntp, supplied by GenBiotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dntp/product/GenBiotech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
dntp - by Bioz Stars, 2020-04
86/100 stars

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DNA Extraction:

Article Title: First Record of Fusarium verticillioides as an Entomopathogenic Fungus of Grasshoppers
Article Snippet: For DNA extraction the isolate was grown on PDA medium at 25 ± 2 °C under a 12 h light-dark photoperiod and 6 day-old cultures were quantitatively scraped from the surfaces of three Petri dishes with a scalpel, frozen in liquid nitrogen, and then ground into a fine powder in a mortar. .. The amplification was carried out in a 25-µl final volume containing 12–15 ng of genomic DNA, 10X reaction buffer (2 mM Tris-HC1 pH 8.0, 10 mM KC1, 0.01 mM EDTA, 1mM DTT, 50% [v/v] glycerol, 0.5% [v/v] Tween 20, 0.5% [v/v] Nonidet P40), 0.5 µM of each primer, 200 µM of each dNTP (Genbiotech S.R.L.), 2.5 mM MgC12, and 1.25 units of Taq DNA polymerase (Genbiotech, www.genbiotech.com ).

Amplification:

Article Title: First Record of Fusarium verticillioides as an Entomopathogenic Fungus of Grasshoppers
Article Snippet: .. The amplification was carried out in a 25-µl final volume containing 12–15 ng of genomic DNA, 10X reaction buffer (2 mM Tris-HC1 pH 8.0, 10 mM KC1, 0.01 mM EDTA, 1mM DTT, 50% [v/v] glycerol, 0.5% [v/v] Tween 20, 0.5% [v/v] Nonidet P40), 0.5 µM of each primer, 200 µM of each dNTP (Genbiotech S.R.L.), 2.5 mM MgC12, and 1.25 units of Taq DNA polymerase (Genbiotech, www.genbiotech.com ). .. DNA amplification was performed in an XP thermal cycler (Bioer Technology Co., www.bioer.com.cn ) with an initial denaturing step at 95° C for 2 min; followed by 29 cycles at 95° C for 30 s, 54° C for 35 s, and 72° C for 45 s; and a final extension cycle at 72° C for 2 min.

Isolation:

Article Title: First Record of Fusarium verticillioides as an Entomopathogenic Fungus of Grasshoppers
Article Snippet: The fungal species isolated from T. collaris were identified on the basis of the macromorphological appearance of the colonies—such as color, diameter, mycelial texture—and their micromorphological characteristics were observed under phase-contrast microscopy. .. The amplification was carried out in a 25-µl final volume containing 12–15 ng of genomic DNA, 10X reaction buffer (2 mM Tris-HC1 pH 8.0, 10 mM KC1, 0.01 mM EDTA, 1mM DTT, 50% [v/v] glycerol, 0.5% [v/v] Tween 20, 0.5% [v/v] Nonidet P40), 0.5 µM of each primer, 200 µM of each dNTP (Genbiotech S.R.L.), 2.5 mM MgC12, and 1.25 units of Taq DNA polymerase (Genbiotech, www.genbiotech.com ).

Microscopy:

Article Title: First Record of Fusarium verticillioides as an Entomopathogenic Fungus of Grasshoppers
Article Snippet: The fungal species isolated from T. collaris were identified on the basis of the macromorphological appearance of the colonies—such as color, diameter, mycelial texture—and their micromorphological characteristics were observed under phase-contrast microscopy. .. The amplification was carried out in a 25-µl final volume containing 12–15 ng of genomic DNA, 10X reaction buffer (2 mM Tris-HC1 pH 8.0, 10 mM KC1, 0.01 mM EDTA, 1mM DTT, 50% [v/v] glycerol, 0.5% [v/v] Tween 20, 0.5% [v/v] Nonidet P40), 0.5 µM of each primer, 200 µM of each dNTP (Genbiotech S.R.L.), 2.5 mM MgC12, and 1.25 units of Taq DNA polymerase (Genbiotech, www.genbiotech.com ).

Electrophoresis:

Article Title: First Record of Fusarium verticillioides as an Entomopathogenic Fungus of Grasshoppers
Article Snippet: The amplification was carried out in a 25-µl final volume containing 12–15 ng of genomic DNA, 10X reaction buffer (2 mM Tris-HC1 pH 8.0, 10 mM KC1, 0.01 mM EDTA, 1mM DTT, 50% [v/v] glycerol, 0.5% [v/v] Tween 20, 0.5% [v/v] Nonidet P40), 0.5 µM of each primer, 200 µM of each dNTP (Genbiotech S.R.L.), 2.5 mM MgC12, and 1.25 units of Taq DNA polymerase (Genbiotech, www.genbiotech.com ). .. The specific product of 800 bp was examined by electrophoresis in 1.5% (w/v) agarose gels containing GelRedTM (Genbiotech) at 80 V in 5X Trisborate-EDTA buffer for 3–4 h at room temperature.

Polymerase Chain Reaction:

Article Title: First Record of Fusarium verticillioides as an Entomopathogenic Fungus of Grasshoppers
Article Snippet: A F. verticillioides -specific PCR was performed with primers 5′-GTCAGAATCCATGCCA GAACG-3′-forward and 5′- CACCCGCAGC AATCCATCAG-3′- reverse ( ). .. The amplification was carried out in a 25-µl final volume containing 12–15 ng of genomic DNA, 10X reaction buffer (2 mM Tris-HC1 pH 8.0, 10 mM KC1, 0.01 mM EDTA, 1mM DTT, 50% [v/v] glycerol, 0.5% [v/v] Tween 20, 0.5% [v/v] Nonidet P40), 0.5 µM of each primer, 200 µM of each dNTP (Genbiotech S.R.L.), 2.5 mM MgC12, and 1.25 units of Taq DNA polymerase (Genbiotech, www.genbiotech.com ).

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    GenBiotech dntps mix
    Dntps Mix, supplied by GenBiotech, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dntps mix/product/GenBiotech
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    dntps mix - by Bioz Stars, 2020-04
    92/100 stars
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