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Feldan dntp
Dntp, supplied by Feldan, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dntp/product/Feldan
Average 91 stars, based on 6 article reviews
Price from $9.99 to $1999.99
dntp - by Bioz Stars, 2020-03
91/100 stars

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Amplification:

Article Title: Vertical distribution of microbial communities in a perennially stratified Arctic lake with saline, anoxic bottom waters
Article Snippet: Paragraph title: SSU rRNA gene amplification, 454 pyrosequencing and clone library verification ... Briefly, PCR reactions contained: 1X HF buffer (NEB), 200 µM of each dNTP (Feldan Bio), 0.4 mg/mL BSA (Fermentas), 0.2 µM of each 454 primer (Invitrogen), 1 U of Phusion High-Fidelity DNA polymerase (NEB), and 1–3 µL of template DNA.

Article Title: Arctic Ocean Microbial Community Structure before and after the 2007 Record Sea Ice Minimum
Article Snippet: Paragraph title: SSU rDNA gene amplification and 454 pyrosequencing ... PCR reactions contained: 1X HF buffer (NEB), 200 µM of each dNTP (Feldan Bio), 0.4 mg/mL BSA (Fermentas), 0.2 µM of each 454 primer (Invitrogen), 1 U of Phusion High-Fidelity DNA polymerase (NEB), and 1–3 µL of template DNA.

Article Title: Small Thaw Ponds: An Unaccounted Source of Methane in the Canadian High Arctic
Article Snippet: A PCR reaction mixture of 1× HF buffer (NEB), 200 µM dNTP (Feldan Bio), 0.4 mg mL−1 BSA (Fermentas), 0.2 µM of each 454 primer (969F: ACGCGHNRAACCTTACC and 1401R: CRGTGWGTRCAAGGRGCA) , 1 U of Phusion High-Fidelity DNA polymerase (NEB), and 0.1–1 µL of template DNA for sediment samples, or 2 µL for water samples. .. Amplification cycles included denaturing at 98°C for 30 s, 30 cycles of denaturing at 98°C for 10 s, annealing at 55°C for 30 s, extension at 72°C for 30 s, and a final extension at 72°C for 5 min. For each sample, the triplicate reactions were pooled together for purification (QIAquick PCR purification kit; QIAGEN) and quantification (Nanodrop ND-1000).

Isolation:

Article Title: Small Thaw Ponds: An Unaccounted Source of Methane in the Canadian High Arctic
Article Snippet: DNA was extracted using the MO BIO Kit (RNA Powersoil total RNA isolation kit #12866-25 and DNA elution accessory kit #12867-25) allowing both RNA and DNA to be extracted at the same time, but only DNA was sequenced for this study. .. A PCR reaction mixture of 1× HF buffer (NEB), 200 µM dNTP (Feldan Bio), 0.4 mg mL−1 BSA (Fermentas), 0.2 µM of each 454 primer (969F: ACGCGHNRAACCTTACC and 1401R: CRGTGWGTRCAAGGRGCA) , 1 U of Phusion High-Fidelity DNA polymerase (NEB), and 0.1–1 µL of template DNA for sediment samples, or 2 µL for water samples.

Spectrophotometry:

Article Title: Exacerbation induces a microbiota shift in sputa of COPD patients
Article Snippet: PCR reactions contained: 1X Q5 buffer (New England Biolabs, Ipswich, Massachusetts, USA), 200 μM of each dNTP (Feldan, Quebec, Quebec, Canada), 0.2 μM of each 454 primer (IDT), 1 U of Q5 High-Fidelity DNA polymerase (NEB), and 1 μL of template DNA (10–50 ng). .. PCR products were then quantified with a NanoDrop 2000 spectrophotometer (Thermo Scientific).

Article Title: Small Thaw Ponds: An Unaccounted Source of Methane in the Canadian High Arctic
Article Snippet: Cellular DNA was extracted from both filters, with a phenol∶chloroform∶Indole-3-Acetic Acid (25∶24∶1) and chloroform∶Indole-3-Acetic Acid (24∶1) separation and DNA quantified by spectrophotometry (Nanodrop ND-1000). .. A PCR reaction mixture of 1× HF buffer (NEB), 200 µM dNTP (Feldan Bio), 0.4 mg mL−1 BSA (Fermentas), 0.2 µM of each 454 primer (969F: ACGCGHNRAACCTTACC and 1401R: CRGTGWGTRCAAGGRGCA) , 1 U of Phusion High-Fidelity DNA polymerase (NEB), and 0.1–1 µL of template DNA for sediment samples, or 2 µL for water samples.

Purification:

Article Title: Vertical distribution of microbial communities in a perennially stratified Arctic lake with saline, anoxic bottom waters
Article Snippet: Briefly, PCR reactions contained: 1X HF buffer (NEB), 200 µM of each dNTP (Feldan Bio), 0.4 mg/mL BSA (Fermentas), 0.2 µM of each 454 primer (Invitrogen), 1 U of Phusion High-Fidelity DNA polymerase (NEB), and 1–3 µL of template DNA. .. The triplicate reactions for each Domain were pooled, purified using the QIAquick PCR purification kit (QIAGEN) and quantified spectrophotometrically (Nanodrop ND-1000).

Article Title: Exacerbation induces a microbiota shift in sputa of COPD patients
Article Snippet: PCR reactions contained: 1X Q5 buffer (New England Biolabs, Ipswich, Massachusetts, USA), 200 μM of each dNTP (Feldan, Quebec, Quebec, Canada), 0.2 μM of each 454 primer (IDT), 1 U of Q5 High-Fidelity DNA polymerase (NEB), and 1 μL of template DNA (10–50 ng). .. Cycling conditions were as follows: an initial denaturation at 98°C for 30 s, followed by 30 cycles of denaturation at 98°C for 10 s, annealing at 55°C for 30 s, extension at 72°C for 30 s, and a final extension at 72°C for 5 min. PCR products were purified using the Axyprep Mag PCR Clean-up Kit (Axygen, Union City, California, USA), and verified with a Bioanalyzer 2100 with DNA 7500 chips (Agilent Technologies, Santa Clara, California, USA).

Article Title: Arctic Ocean Microbial Community Structure before and after the 2007 Record Sea Ice Minimum
Article Snippet: PCR reactions contained: 1X HF buffer (NEB), 200 µM of each dNTP (Feldan Bio), 0.4 mg/mL BSA (Fermentas), 0.2 µM of each 454 primer (Invitrogen), 1 U of Phusion High-Fidelity DNA polymerase (NEB), and 1–3 µL of template DNA. .. The triplicate reactions for the separate domains were pooled, purified using the QIAquick PCR purification kit (QIAGEN), and quantified spectrophotometrically (Nanodrop ND-1000).

Article Title: Small Thaw Ponds: An Unaccounted Source of Methane in the Canadian High Arctic
Article Snippet: A PCR reaction mixture of 1× HF buffer (NEB), 200 µM dNTP (Feldan Bio), 0.4 mg mL−1 BSA (Fermentas), 0.2 µM of each 454 primer (969F: ACGCGHNRAACCTTACC and 1401R: CRGTGWGTRCAAGGRGCA) , 1 U of Phusion High-Fidelity DNA polymerase (NEB), and 0.1–1 µL of template DNA for sediment samples, or 2 µL for water samples. .. Amplification cycles included denaturing at 98°C for 30 s, 30 cycles of denaturing at 98°C for 10 s, annealing at 55°C for 30 s, extension at 72°C for 30 s, and a final extension at 72°C for 5 min. For each sample, the triplicate reactions were pooled together for purification (QIAquick PCR purification kit; QIAGEN) and quantification (Nanodrop ND-1000).

SYBR Green Assay:

Article Title: Adipose tissue (P)RR regulates insulin sensitivity, fat mass and body weight
Article Snippet: Each reaction contained 1 μl 10× buffer, 0.2 μl 10 mM dNTP, 0.1 μl of each primer, 6.4 μl of water, 0.5 μl Taq polymerase (Feldan, Bio-Basic, Markham, ON, Canada), and 2 μl of genomic DNA, as described previously . .. PCR products were subsequently analyzed on 1% or 3% agarose gels containing SYBR Green (Invitrogen by Life Technologies, Carlsbad, CA, USA).

Polymerase Chain Reaction:

Article Title: Vertical distribution of microbial communities in a perennially stratified Arctic lake with saline, anoxic bottom waters
Article Snippet: .. Briefly, PCR reactions contained: 1X HF buffer (NEB), 200 µM of each dNTP (Feldan Bio), 0.4 mg/mL BSA (Fermentas), 0.2 µM of each 454 primer (Invitrogen), 1 U of Phusion High-Fidelity DNA polymerase (NEB), and 1–3 µL of template DNA. ..

Article Title: Exacerbation induces a microbiota shift in sputa of COPD patients
Article Snippet: .. PCR reactions contained: 1X Q5 buffer (New England Biolabs, Ipswich, Massachusetts, USA), 200 μM of each dNTP (Feldan, Quebec, Quebec, Canada), 0.2 μM of each 454 primer (IDT), 1 U of Q5 High-Fidelity DNA polymerase (NEB), and 1 μL of template DNA (10–50 ng). .. Cycling conditions were as follows: an initial denaturation at 98°C for 30 s, followed by 30 cycles of denaturation at 98°C for 10 s, annealing at 55°C for 30 s, extension at 72°C for 30 s, and a final extension at 72°C for 5 min. PCR products were purified using the Axyprep Mag PCR Clean-up Kit (Axygen, Union City, California, USA), and verified with a Bioanalyzer 2100 with DNA 7500 chips (Agilent Technologies, Santa Clara, California, USA).

Article Title: Arctic Ocean Microbial Community Structure before and after the 2007 Record Sea Ice Minimum
Article Snippet: .. PCR reactions contained: 1X HF buffer (NEB), 200 µM of each dNTP (Feldan Bio), 0.4 mg/mL BSA (Fermentas), 0.2 µM of each 454 primer (Invitrogen), 1 U of Phusion High-Fidelity DNA polymerase (NEB), and 1–3 µL of template DNA. ..

Article Title: Small Thaw Ponds: An Unaccounted Source of Methane in the Canadian High Arctic
Article Snippet: .. A PCR reaction mixture of 1× HF buffer (NEB), 200 µM dNTP (Feldan Bio), 0.4 mg mL−1 BSA (Fermentas), 0.2 µM of each 454 primer (969F: ACGCGHNRAACCTTACC and 1401R: CRGTGWGTRCAAGGRGCA) , 1 U of Phusion High-Fidelity DNA polymerase (NEB), and 0.1–1 µL of template DNA for sediment samples, or 2 µL for water samples. .. Three separate DNA concentrations were used for each sample, from 1× to 2.22×, to reduce PCR bias.

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  • 91
    Feldan dntps
    Dntps, supplied by Feldan, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dntps/product/Feldan
    Average 91 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    dntps - by Bioz Stars, 2020-03
    91/100 stars
      Buy from Supplier

    99
    Feldan deoxynucleoside triphosphates dntps
    Deoxynucleoside Triphosphates Dntps, supplied by Feldan, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphates dntps/product/Feldan
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphates dntps - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

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