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Amresco dntp
Dntp, supplied by Amresco, used in various techniques. Bioz Stars score: 96/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 8 article reviews
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dntp - by Bioz Stars, 2020-03
96/100 stars

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Clone Assay:

Article Title: A multi-locus approach to barcoding in the Anopheles strodei subgroup (Diptera: Culicidae)
Article Snippet: The PCR was carried out in a 25-μL aqueous reaction mixture containing 1 μL of DNA extraction solution, 1X PCR buffer (Invitrogen), 1.5 mM MgCl2 (Invitrogen), 1.25 μL dimethyl sulfoxide (Sigma), 0.1 μM of each primer, 0.2 mM each dNTP (Amresco) and 1.25 U Taq Platinum polymerase (Invitrogen). .. The reaction proceeded under the following temperature profile: 94°C for 2 min, 34 cycles of 94°C for 30 s, 57°C for 30 s and 72°C for 30 s and a final extension at 72°C for 10 min. ITS2 amplicons that yielded ambiguous sequence chromatograms, which is suggestive of intragenomic variation, were purified using PEG precipitation (20% polyethylene glycol 8,000/2.5 M NaCl) and then cloned into pGem-T Easy Vector (Promega, Madison, WI).

Article Title: A multi-locus approach to barcoding in the Anopheles strodei subgroup (Diptera: Culicidae)
Article Snippet: Both PCRs were carried out in a 25-μL aqueous reaction mixture containing 1 μl DNA extraction solution, 1X PCR buffer (Invitrogen), 1.5 mM MgCl2 (Invitrogen), 2.5 μL of dimethyl sulfoxide (Sigma), 2.0 μM of each primer, 0.2 mM each dNTP (Amresco) and 2.5 U Taq Platinum polymerase (Invitrogen). .. Both PCRs proceeded under the following temperature profile: 94°C for 5 min, 35 cycles at 94°C for 30 s, an annealing temperature of 50°C for 1 min and then 72°C for 2 min followed by a final extension at 72°C for 10 min. Any white amplicons that yielded ambiguous sequence chromatograms were purified using PEG precipitation (20% polyethylene glycol 8,000/2.5 M NaCl) and then cloned into pGem-T Easy Vector (Promega).

Amplification:

Article Title: Detection and genotyping of Helicobacter pylori in saliva versus stool samples from asymptomatic individuals in Northeastern Thailand reveals intra-host tissue-specific H. pylori subtypes
Article Snippet: vac A of H. pylori was amplified by semi-nested PCR using specific primers, vac F1/F2 and R1 followed by vac F1/F2 and R2 (Table ). .. PCR was performed in a total volume of 25 μl containing 500 ng DNA template, 1X PCR buffer (+ 1.5 mM MgCl2 ), 0.4 μM of each primer, 0.5 unit of Taq DNA polymerase (RBC bioscience, Taipei, Taiwan) and 0.2 mM dNTP (Amresco, Ohio, USA) using a thermal cycler (C1000™ Thermal Cycler, BioRad).

Article Title: Gene expression analysis of potential genes and pathways involved in the pathogenic mechanisms of parvovirus B19 in human colorectal cancer
Article Snippet: .. The amplification reaction consisted of 10X PCR buffer, 1.25 units of JumpStart™ Taq (Sigma-Aldrich), 10 pmol forward and reverse primers, 0.2 μmol dNTP, 100 ng template and 0.2X SYBR-Green I (Amresco Inc., Solon, OH, USA) in a final volume of 50 μl. .. The reactions were performed using the StepOneTM Real-Time PCR System (Applied Biosystems, Inc., Foster City, CA, USA).

Article Title: Detection and genotyping of Helicobacter pylori in saliva versus stool samples from asymptomatic individuals in Northeastern Thailand reveals intra-host tissue-specific H. pylori subtypes
Article Snippet: Semi-nested PCR for detection of H. pylori vac A of H. pylori was amplified by semi-nested PCR using specific primers, vac F1/F2 and R1 followed by vac F1/F2 and R2 (Table ). .. PCR was performed in a total volume of 25 μl containing 500 ng DNA template, 1X PCR buffer (+ 1.5 mM MgCl2 ), 0.4 μM of each primer, 0.5 unit of Taq DNA polymerase (RBC bioscience, Taipei, Taiwan) and 0.2 mM dNTP (Amresco, Ohio, USA) using a thermal cycler (C1000™ Thermal Cycler, BioRad).

Article Title: A multi-locus approach to barcoding in the Anopheles strodei subgroup (Diptera: Culicidae)
Article Snippet: ITS2 region This region was amplified using 5.8SF (5′-ATC ACT CGG CTC GTG GAT CG-3′) and 28SR (5′-ATG CTT AAA TTT AGG GGG TAG TC-3′) primers [ ]. .. The PCR was carried out in a 25-μL aqueous reaction mixture containing 1 μL of DNA extraction solution, 1X PCR buffer (Invitrogen), 1.5 mM MgCl2 (Invitrogen), 1.25 μL dimethyl sulfoxide (Sigma), 0.1 μM of each primer, 0.2 mM each dNTP (Amresco) and 1.25 U Taq Platinum polymerase (Invitrogen).

Article Title: A multi-locus approach to barcoding in the Anopheles strodei subgroup (Diptera: Culicidae)
Article Snippet: This amplification product then served as a template in a sequencing reaction using internal primers W1F (5′-GAT CAA RAA GAT CTG YGA CTC GTT-3′) and W2R (5′GCC ATC GAG ATG GAG GAG CTG-3′). .. Both PCRs were carried out in a 25-μL aqueous reaction mixture containing 1 μl DNA extraction solution, 1X PCR buffer (Invitrogen), 1.5 mM MgCl2 (Invitrogen), 2.5 μL of dimethyl sulfoxide (Sigma), 2.0 μM of each primer, 0.2 mM each dNTP (Amresco) and 2.5 U Taq Platinum polymerase (Invitrogen).

Article Title: A multi-locus approach to barcoding in the Anopheles strodei subgroup (Diptera: Culicidae)
Article Snippet: COI gene The gene was amplified using LCO- 1490 (5′-GGT CAA CAA ATC ATA AAG ATA TTG G-3′) and HCO-2198 (5′-TAA ACT TCA GGG TGA CCA AAA ATC A-3′) primers [ ]. .. The Polymerase Chain Reaction (PCR) was carried out in a 25-μL aqueous reaction mixture containing 1 μL of DNA extraction solution, 1X PCR buffer (Invitrogen, Carlsbad, CA, USA), 1.5 mM MgCl2 (Invitrogen), 1.25 μL dimethly sulfoxide (Sigma, St. Louis, MO, USA), 0.1 μM of each primer, 0.2 mM each dNTP (Amresco, Solon, OH, USA) and 1.25 U Taq Platinum polymerase (Invitrogen).

Quantitative RT-PCR:

Article Title: Alteration of colonic excitatory tachykininergic motility and enteric inflammation following dopaminergic nigrostriatal neurodegeneration
Article Snippet: Paragraph title: RNA extraction and RT-qPCR ... RT was performed in a mixture of 2 μL RT buffer, 0.8 μL dNTP, 2 μL random primer, 1 μL reverse transcriptase, and nuclease-free water (Amresco LLC, Solon, USA) up to 10 μL.

Electrophoresis:

Article Title: Detection and genotyping of Helicobacter pylori in saliva versus stool samples from asymptomatic individuals in Northeastern Thailand reveals intra-host tissue-specific H. pylori subtypes
Article Snippet: PCR was performed in a total volume of 25 μl containing 500 ng DNA template, 1X PCR buffer (+ 1.5 mM MgCl2 ), 0.4 μM of each primer, 0.5 unit of Taq DNA polymerase (RBC bioscience, Taipei, Taiwan) and 0.2 mM dNTP (Amresco, Ohio, USA) using a thermal cycler (C1000™ Thermal Cycler, BioRad). .. Amplified products were separated by electrophoresis in a 1.5% agarose gel and visualized by staining with ethidium bromide.

Article Title: Detection and genotyping of Helicobacter pylori in saliva versus stool samples from asymptomatic individuals in Northeastern Thailand reveals intra-host tissue-specific H. pylori subtypes
Article Snippet: PCR was performed in a total volume of 25 μl containing 500 ng DNA template, 1X PCR buffer (+ 1.5 mM MgCl2 ), 0.4 μM of each primer, 0.5 unit of Taq DNA polymerase (RBC bioscience, Taipei, Taiwan) and 0.2 mM dNTP (Amresco, Ohio, USA) using a thermal cycler (C1000™ Thermal Cycler, BioRad). .. Amplified products were separated by electrophoresis in a 1.5% agarose gel and visualized by staining with ethidium bromide.

Microarray:

Article Title: Gene expression analysis of potential genes and pathways involved in the pathogenic mechanisms of parvovirus B19 in human colorectal cancer
Article Snippet: The expression of the 12 genes that were identified as being associated with apoptosis in the microarray analysis was determined using qPCR analysis with SYBR-Green I (Invitrogen Life Technologies). .. The amplification reaction consisted of 10X PCR buffer, 1.25 units of JumpStart™ Taq (Sigma-Aldrich), 10 pmol forward and reverse primers, 0.2 μmol dNTP, 100 ng template and 0.2X SYBR-Green I (Amresco Inc., Solon, OH, USA) in a final volume of 50 μl.

Expressing:

Article Title: Gene expression analysis of potential genes and pathways involved in the pathogenic mechanisms of parvovirus B19 in human colorectal cancer
Article Snippet: The expression of the 12 genes that were identified as being associated with apoptosis in the microarray analysis was determined using qPCR analysis with SYBR-Green I (Invitrogen Life Technologies). .. The amplification reaction consisted of 10X PCR buffer, 1.25 units of JumpStart™ Taq (Sigma-Aldrich), 10 pmol forward and reverse primers, 0.2 μmol dNTP, 100 ng template and 0.2X SYBR-Green I (Amresco Inc., Solon, OH, USA) in a final volume of 50 μl.

Article Title: Alteration of colonic excitatory tachykininergic motility and enteric inflammation following dopaminergic nigrostriatal neurodegeneration
Article Snippet: RT was performed in a mixture of 2 μL RT buffer, 0.8 μL dNTP, 2 μL random primer, 1 μL reverse transcriptase, and nuclease-free water (Amresco LLC, Solon, USA) up to 10 μL. .. Expression values were normalized to the housekeeping gene 18S expression.

Real-time Polymerase Chain Reaction:

Article Title: Gene expression analysis of potential genes and pathways involved in the pathogenic mechanisms of parvovirus B19 in human colorectal cancer
Article Snippet: The expression of the 12 genes that were identified as being associated with apoptosis in the microarray analysis was determined using qPCR analysis with SYBR-Green I (Invitrogen Life Technologies). .. The amplification reaction consisted of 10X PCR buffer, 1.25 units of JumpStart™ Taq (Sigma-Aldrich), 10 pmol forward and reverse primers, 0.2 μmol dNTP, 100 ng template and 0.2X SYBR-Green I (Amresco Inc., Solon, OH, USA) in a final volume of 50 μl.

Article Title: Alteration of colonic excitatory tachykininergic motility and enteric inflammation following dopaminergic nigrostriatal neurodegeneration
Article Snippet: RT was performed in a mixture of 2 μL RT buffer, 0.8 μL dNTP, 2 μL random primer, 1 μL reverse transcriptase, and nuclease-free water (Amresco LLC, Solon, USA) up to 10 μL. .. PCR cycles (Veriti 96-Well Thermal Cycler, Applied Biosystems, Thermo Fisher Scientific Inc., CA, USA) were set as follows: 25 °C for 10 min, 37 °C for 120 min, and 85 °C for 5 min. Tenfold dilutions of complementary DNA (cDNA) specimens were used for the real-time quantitative PCR analysis (RT-qPCR) procedure. qPCR reactions were performed with the following primers: inducible nitric oxide synthase (iNOS) 5′-CCTTGTTCAGCTACGCCTTC-3′ (sense) and 5′-CCAGGCCAAATACCGCATAC-3′ (anti-sense); arginase-1 5′-GGACATCGTGTACATCGGCT-3′ (sense) and 5′-GGGCCTTTTCTTCCTTCCCA-3′ (anti-sense); 18S 5′-GGGAGCCTGAGAAACGGC-3′ (sense) and 5′-GGGTCGGGAGTGGGTAATTTT-3′ (anti-sense).

Transfection:

Article Title: Gene expression analysis of potential genes and pathways involved in the pathogenic mechanisms of parvovirus B19 in human colorectal cancer
Article Snippet: Total RNA was extracted from the transfected cells. .. The amplification reaction consisted of 10X PCR buffer, 1.25 units of JumpStart™ Taq (Sigma-Aldrich), 10 pmol forward and reverse primers, 0.2 μmol dNTP, 100 ng template and 0.2X SYBR-Green I (Amresco Inc., Solon, OH, USA) in a final volume of 50 μl.

Polymerase Chain Reaction:

Article Title: Detection and genotyping of Helicobacter pylori in saliva versus stool samples from asymptomatic individuals in Northeastern Thailand reveals intra-host tissue-specific H. pylori subtypes
Article Snippet: .. PCR was performed in a total volume of 25 μl containing 500 ng DNA template, 1X PCR buffer (+ 1.5 mM MgCl2 ), 0.4 μM of each primer, 0.5 unit of Taq DNA polymerase (RBC bioscience, Taipei, Taiwan) and 0.2 mM dNTP (Amresco, Ohio, USA) using a thermal cycler (C1000™ Thermal Cycler, BioRad). .. PCR conditions and primer sequences are shown in Table .

Article Title: Gene expression analysis of potential genes and pathways involved in the pathogenic mechanisms of parvovirus B19 in human colorectal cancer
Article Snippet: .. The amplification reaction consisted of 10X PCR buffer, 1.25 units of JumpStart™ Taq (Sigma-Aldrich), 10 pmol forward and reverse primers, 0.2 μmol dNTP, 100 ng template and 0.2X SYBR-Green I (Amresco Inc., Solon, OH, USA) in a final volume of 50 μl. .. The reactions were performed using the StepOneTM Real-Time PCR System (Applied Biosystems, Inc., Foster City, CA, USA).

Article Title: Alteration of colonic excitatory tachykininergic motility and enteric inflammation following dopaminergic nigrostriatal neurodegeneration
Article Snippet: RT was performed in a mixture of 2 μL RT buffer, 0.8 μL dNTP, 2 μL random primer, 1 μL reverse transcriptase, and nuclease-free water (Amresco LLC, Solon, USA) up to 10 μL. .. PCR cycles (Veriti 96-Well Thermal Cycler, Applied Biosystems, Thermo Fisher Scientific Inc., CA, USA) were set as follows: 25 °C for 10 min, 37 °C for 120 min, and 85 °C for 5 min. Tenfold dilutions of complementary DNA (cDNA) specimens were used for the real-time quantitative PCR analysis (RT-qPCR) procedure. qPCR reactions were performed with the following primers: inducible nitric oxide synthase (iNOS) 5′-CCTTGTTCAGCTACGCCTTC-3′ (sense) and 5′-CCAGGCCAAATACCGCATAC-3′ (anti-sense); arginase-1 5′-GGACATCGTGTACATCGGCT-3′ (sense) and 5′-GGGCCTTTTCTTCCTTCCCA-3′ (anti-sense); 18S 5′-GGGAGCCTGAGAAACGGC-3′ (sense) and 5′-GGGTCGGGAGTGGGTAATTTT-3′ (anti-sense).

Article Title: Detection and genotyping of Helicobacter pylori in saliva versus stool samples from asymptomatic individuals in Northeastern Thailand reveals intra-host tissue-specific H. pylori subtypes
Article Snippet: .. PCR was performed in a total volume of 25 μl containing 500 ng DNA template, 1X PCR buffer (+ 1.5 mM MgCl2 ), 0.4 μM of each primer, 0.5 unit of Taq DNA polymerase (RBC bioscience, Taipei, Taiwan) and 0.2 mM dNTP (Amresco, Ohio, USA) using a thermal cycler (C1000™ Thermal Cycler, BioRad). .. PCR conditions and primer sequences are shown in Table .

Article Title: A multi-locus approach to barcoding in the Anopheles strodei subgroup (Diptera: Culicidae)
Article Snippet: .. The PCR was carried out in a 25-μL aqueous reaction mixture containing 1 μL of DNA extraction solution, 1X PCR buffer (Invitrogen), 1.5 mM MgCl2 (Invitrogen), 1.25 μL dimethyl sulfoxide (Sigma), 0.1 μM of each primer, 0.2 mM each dNTP (Amresco) and 1.25 U Taq Platinum polymerase (Invitrogen). .. The reaction proceeded under the following temperature profile: 94°C for 2 min, 34 cycles of 94°C for 30 s, 57°C for 30 s and 72°C for 30 s and a final extension at 72°C for 10 min. ITS2 amplicons that yielded ambiguous sequence chromatograms, which is suggestive of intragenomic variation, were purified using PEG precipitation (20% polyethylene glycol 8,000/2.5 M NaCl) and then cloned into pGem-T Easy Vector (Promega, Madison, WI).

Article Title: A multi-locus approach to barcoding in the Anopheles strodei subgroup (Diptera: Culicidae)
Article Snippet: .. Both PCRs were carried out in a 25-μL aqueous reaction mixture containing 1 μl DNA extraction solution, 1X PCR buffer (Invitrogen), 1.5 mM MgCl2 (Invitrogen), 2.5 μL of dimethyl sulfoxide (Sigma), 2.0 μM of each primer, 0.2 mM each dNTP (Amresco) and 2.5 U Taq Platinum polymerase (Invitrogen). .. Both PCRs proceeded under the following temperature profile: 94°C for 5 min, 35 cycles at 94°C for 30 s, an annealing temperature of 50°C for 1 min and then 72°C for 2 min followed by a final extension at 72°C for 10 min. Any white amplicons that yielded ambiguous sequence chromatograms were purified using PEG precipitation (20% polyethylene glycol 8,000/2.5 M NaCl) and then cloned into pGem-T Easy Vector (Promega).

Article Title: A multi-locus approach to barcoding in the Anopheles strodei subgroup (Diptera: Culicidae)
Article Snippet: .. The Polymerase Chain Reaction (PCR) was carried out in a 25-μL aqueous reaction mixture containing 1 μL of DNA extraction solution, 1X PCR buffer (Invitrogen, Carlsbad, CA, USA), 1.5 mM MgCl2 (Invitrogen), 1.25 μL dimethly sulfoxide (Sigma, St. Louis, MO, USA), 0.1 μM of each primer, 0.2 mM each dNTP (Amresco, Solon, OH, USA) and 1.25 U Taq Platinum polymerase (Invitrogen). .. ITS2 region This region was amplified using 5.8SF (5′-ATC ACT CGG CTC GTG GAT CG-3′) and 28SR (5′-ATG CTT AAA TTT AGG GGG TAG TC-3′) primers [ ].

Article Title: Neuroglobin-Deficiency Exacerbates Hif1A and c-FOS Response, but Does Not Affect Neuronal Survival during Severe Hypoxia In Vivo
Article Snippet: Genotyping Tail-clip genotyping was performed using the following primer sets in 0.2 µM concentrations: 5′-CTCTCAGGCGTGAGGTAAGG-3′ , 5′-CTGGCTCCCACTGTATTGGT-3′ , 5′-CCCAGA TTCTGATACCCAC-3′ and 5′-GCACTCCTTGCTGAGACCTAG-3′ using 1× DyNazyme reaction buffer and DyNazyme I polymerase (cat. No. F-500S Finnzymes, Finland), 0.25 µM dNTP (Amresco, USA). .. The PCR reaction was run as follows: 94°C 2 min, 92°C 1 min, 35 cycles of 60°C 1 min, 72°C 30 sec, 92°C 1 min, 60°C 1 min, 72°C 10 min.

Cellular Antioxidant Activity Assay:

Article Title: A multi-locus approach to barcoding in the Anopheles strodei subgroup (Diptera: Culicidae)
Article Snippet: This amplification product then served as a template in a sequencing reaction using internal primers W1F (5′-GAT CAA RAA GAT CTG YGA CTC GTT-3′) and W2R (5′GCC ATC GAG ATG GAG GAG CTG-3′). .. Both PCRs were carried out in a 25-μL aqueous reaction mixture containing 1 μl DNA extraction solution, 1X PCR buffer (Invitrogen), 1.5 mM MgCl2 (Invitrogen), 2.5 μL of dimethyl sulfoxide (Sigma), 2.0 μM of each primer, 0.2 mM each dNTP (Amresco) and 2.5 U Taq Platinum polymerase (Invitrogen).

Article Title: A multi-locus approach to barcoding in the Anopheles strodei subgroup (Diptera: Culicidae)
Article Snippet: COI gene The gene was amplified using LCO- 1490 (5′-GGT CAA CAA ATC ATA AAG ATA TTG G-3′) and HCO-2198 (5′-TAA ACT TCA GGG TGA CCA AAA ATC A-3′) primers [ ]. .. The Polymerase Chain Reaction (PCR) was carried out in a 25-μL aqueous reaction mixture containing 1 μL of DNA extraction solution, 1X PCR buffer (Invitrogen, Carlsbad, CA, USA), 1.5 mM MgCl2 (Invitrogen), 1.25 μL dimethly sulfoxide (Sigma, St. Louis, MO, USA), 0.1 μM of each primer, 0.2 mM each dNTP (Amresco, Solon, OH, USA) and 1.25 U Taq Platinum polymerase (Invitrogen).

DNA Extraction:

Article Title: A multi-locus approach to barcoding in the Anopheles strodei subgroup (Diptera: Culicidae)
Article Snippet: .. The PCR was carried out in a 25-μL aqueous reaction mixture containing 1 μL of DNA extraction solution, 1X PCR buffer (Invitrogen), 1.5 mM MgCl2 (Invitrogen), 1.25 μL dimethyl sulfoxide (Sigma), 0.1 μM of each primer, 0.2 mM each dNTP (Amresco) and 1.25 U Taq Platinum polymerase (Invitrogen). .. The reaction proceeded under the following temperature profile: 94°C for 2 min, 34 cycles of 94°C for 30 s, 57°C for 30 s and 72°C for 30 s and a final extension at 72°C for 10 min. ITS2 amplicons that yielded ambiguous sequence chromatograms, which is suggestive of intragenomic variation, were purified using PEG precipitation (20% polyethylene glycol 8,000/2.5 M NaCl) and then cloned into pGem-T Easy Vector (Promega, Madison, WI).

Article Title: A multi-locus approach to barcoding in the Anopheles strodei subgroup (Diptera: Culicidae)
Article Snippet: .. Both PCRs were carried out in a 25-μL aqueous reaction mixture containing 1 μl DNA extraction solution, 1X PCR buffer (Invitrogen), 1.5 mM MgCl2 (Invitrogen), 2.5 μL of dimethyl sulfoxide (Sigma), 2.0 μM of each primer, 0.2 mM each dNTP (Amresco) and 2.5 U Taq Platinum polymerase (Invitrogen). .. Both PCRs proceeded under the following temperature profile: 94°C for 5 min, 35 cycles at 94°C for 30 s, an annealing temperature of 50°C for 1 min and then 72°C for 2 min followed by a final extension at 72°C for 10 min. Any white amplicons that yielded ambiguous sequence chromatograms were purified using PEG precipitation (20% polyethylene glycol 8,000/2.5 M NaCl) and then cloned into pGem-T Easy Vector (Promega).

Article Title: A multi-locus approach to barcoding in the Anopheles strodei subgroup (Diptera: Culicidae)
Article Snippet: .. The Polymerase Chain Reaction (PCR) was carried out in a 25-μL aqueous reaction mixture containing 1 μL of DNA extraction solution, 1X PCR buffer (Invitrogen, Carlsbad, CA, USA), 1.5 mM MgCl2 (Invitrogen), 1.25 μL dimethly sulfoxide (Sigma, St. Louis, MO, USA), 0.1 μM of each primer, 0.2 mM each dNTP (Amresco, Solon, OH, USA) and 1.25 U Taq Platinum polymerase (Invitrogen). .. ITS2 region This region was amplified using 5.8SF (5′-ATC ACT CGG CTC GTG GAT CG-3′) and 28SR (5′-ATG CTT AAA TTT AGG GGG TAG TC-3′) primers [ ].

Isolation:

Article Title: Alteration of colonic excitatory tachykininergic motility and enteric inflammation following dopaminergic nigrostriatal neurodegeneration
Article Snippet: The concentration of isolated RNA was determined with a spectrophotometer, and 2 μg of RNA were used for the reverse transcription (RT) procedure (High Capacity cDNA Reverse Transcription Kit, Applied Biosystems, Thermo Fisher Scientific Inc., CA, USA). .. RT was performed in a mixture of 2 μL RT buffer, 0.8 μL dNTP, 2 μL random primer, 1 μL reverse transcriptase, and nuclease-free water (Amresco LLC, Solon, USA) up to 10 μL.

Negative Control:

Article Title: Gene expression analysis of potential genes and pathways involved in the pathogenic mechanisms of parvovirus B19 in human colorectal cancer
Article Snippet: GAPDH was used as an internal control and distilled water was used as a negative control. .. The amplification reaction consisted of 10X PCR buffer, 1.25 units of JumpStart™ Taq (Sigma-Aldrich), 10 pmol forward and reverse primers, 0.2 μmol dNTP, 100 ng template and 0.2X SYBR-Green I (Amresco Inc., Solon, OH, USA) in a final volume of 50 μl.

Purification:

Article Title: A multi-locus approach to barcoding in the Anopheles strodei subgroup (Diptera: Culicidae)
Article Snippet: The PCR was carried out in a 25-μL aqueous reaction mixture containing 1 μL of DNA extraction solution, 1X PCR buffer (Invitrogen), 1.5 mM MgCl2 (Invitrogen), 1.25 μL dimethyl sulfoxide (Sigma), 0.1 μM of each primer, 0.2 mM each dNTP (Amresco) and 1.25 U Taq Platinum polymerase (Invitrogen). .. The reaction proceeded under the following temperature profile: 94°C for 2 min, 34 cycles of 94°C for 30 s, 57°C for 30 s and 72°C for 30 s and a final extension at 72°C for 10 min. ITS2 amplicons that yielded ambiguous sequence chromatograms, which is suggestive of intragenomic variation, were purified using PEG precipitation (20% polyethylene glycol 8,000/2.5 M NaCl) and then cloned into pGem-T Easy Vector (Promega, Madison, WI).

Article Title: A multi-locus approach to barcoding in the Anopheles strodei subgroup (Diptera: Culicidae)
Article Snippet: Both PCRs were carried out in a 25-μL aqueous reaction mixture containing 1 μl DNA extraction solution, 1X PCR buffer (Invitrogen), 1.5 mM MgCl2 (Invitrogen), 2.5 μL of dimethyl sulfoxide (Sigma), 2.0 μM of each primer, 0.2 mM each dNTP (Amresco) and 2.5 U Taq Platinum polymerase (Invitrogen). .. Both PCRs proceeded under the following temperature profile: 94°C for 5 min, 35 cycles at 94°C for 30 s, an annealing temperature of 50°C for 1 min and then 72°C for 2 min followed by a final extension at 72°C for 10 min. Any white amplicons that yielded ambiguous sequence chromatograms were purified using PEG precipitation (20% polyethylene glycol 8,000/2.5 M NaCl) and then cloned into pGem-T Easy Vector (Promega).

Sequencing:

Article Title: A multi-locus approach to barcoding in the Anopheles strodei subgroup (Diptera: Culicidae)
Article Snippet: The PCR was carried out in a 25-μL aqueous reaction mixture containing 1 μL of DNA extraction solution, 1X PCR buffer (Invitrogen), 1.5 mM MgCl2 (Invitrogen), 1.25 μL dimethyl sulfoxide (Sigma), 0.1 μM of each primer, 0.2 mM each dNTP (Amresco) and 1.25 U Taq Platinum polymerase (Invitrogen). .. The reaction proceeded under the following temperature profile: 94°C for 2 min, 34 cycles of 94°C for 30 s, 57°C for 30 s and 72°C for 30 s and a final extension at 72°C for 10 min. ITS2 amplicons that yielded ambiguous sequence chromatograms, which is suggestive of intragenomic variation, were purified using PEG precipitation (20% polyethylene glycol 8,000/2.5 M NaCl) and then cloned into pGem-T Easy Vector (Promega, Madison, WI).

Article Title: A multi-locus approach to barcoding in the Anopheles strodei subgroup (Diptera: Culicidae)
Article Snippet: This amplification product then served as a template in a sequencing reaction using internal primers W1F (5′-GAT CAA RAA GAT CTG YGA CTC GTT-3′) and W2R (5′GCC ATC GAG ATG GAG GAG CTG-3′). .. Both PCRs were carried out in a 25-μL aqueous reaction mixture containing 1 μl DNA extraction solution, 1X PCR buffer (Invitrogen), 1.5 mM MgCl2 (Invitrogen), 2.5 μL of dimethyl sulfoxide (Sigma), 2.0 μM of each primer, 0.2 mM each dNTP (Amresco) and 2.5 U Taq Platinum polymerase (Invitrogen).

Activated Clotting Time Assay:

Article Title: A multi-locus approach to barcoding in the Anopheles strodei subgroup (Diptera: Culicidae)
Article Snippet: ITS2 region This region was amplified using 5.8SF (5′-ATC ACT CGG CTC GTG GAT CG-3′) and 28SR (5′-ATG CTT AAA TTT AGG GGG TAG TC-3′) primers [ ]. .. The PCR was carried out in a 25-μL aqueous reaction mixture containing 1 μL of DNA extraction solution, 1X PCR buffer (Invitrogen), 1.5 mM MgCl2 (Invitrogen), 1.25 μL dimethyl sulfoxide (Sigma), 0.1 μM of each primer, 0.2 mM each dNTP (Amresco) and 1.25 U Taq Platinum polymerase (Invitrogen).

Article Title: A multi-locus approach to barcoding in the Anopheles strodei subgroup (Diptera: Culicidae)
Article Snippet: COI gene The gene was amplified using LCO- 1490 (5′-GGT CAA CAA ATC ATA AAG ATA TTG G-3′) and HCO-2198 (5′-TAA ACT TCA GGG TGA CCA AAA ATC A-3′) primers [ ]. .. The Polymerase Chain Reaction (PCR) was carried out in a 25-μL aqueous reaction mixture containing 1 μL of DNA extraction solution, 1X PCR buffer (Invitrogen, Carlsbad, CA, USA), 1.5 mM MgCl2 (Invitrogen), 1.25 μL dimethly sulfoxide (Sigma, St. Louis, MO, USA), 0.1 μM of each primer, 0.2 mM each dNTP (Amresco, Solon, OH, USA) and 1.25 U Taq Platinum polymerase (Invitrogen).

Plasmid Preparation:

Article Title: A multi-locus approach to barcoding in the Anopheles strodei subgroup (Diptera: Culicidae)
Article Snippet: The PCR was carried out in a 25-μL aqueous reaction mixture containing 1 μL of DNA extraction solution, 1X PCR buffer (Invitrogen), 1.5 mM MgCl2 (Invitrogen), 1.25 μL dimethyl sulfoxide (Sigma), 0.1 μM of each primer, 0.2 mM each dNTP (Amresco) and 1.25 U Taq Platinum polymerase (Invitrogen). .. The reaction proceeded under the following temperature profile: 94°C for 2 min, 34 cycles of 94°C for 30 s, 57°C for 30 s and 72°C for 30 s and a final extension at 72°C for 10 min. ITS2 amplicons that yielded ambiguous sequence chromatograms, which is suggestive of intragenomic variation, were purified using PEG precipitation (20% polyethylene glycol 8,000/2.5 M NaCl) and then cloned into pGem-T Easy Vector (Promega, Madison, WI).

Article Title: A multi-locus approach to barcoding in the Anopheles strodei subgroup (Diptera: Culicidae)
Article Snippet: Both PCRs were carried out in a 25-μL aqueous reaction mixture containing 1 μl DNA extraction solution, 1X PCR buffer (Invitrogen), 1.5 mM MgCl2 (Invitrogen), 2.5 μL of dimethyl sulfoxide (Sigma), 2.0 μM of each primer, 0.2 mM each dNTP (Amresco) and 2.5 U Taq Platinum polymerase (Invitrogen). .. Both PCRs proceeded under the following temperature profile: 94°C for 5 min, 35 cycles at 94°C for 30 s, an annealing temperature of 50°C for 1 min and then 72°C for 2 min followed by a final extension at 72°C for 10 min. Any white amplicons that yielded ambiguous sequence chromatograms were purified using PEG precipitation (20% polyethylene glycol 8,000/2.5 M NaCl) and then cloned into pGem-T Easy Vector (Promega).

SYBR Green Assay:

Article Title: Gene expression analysis of potential genes and pathways involved in the pathogenic mechanisms of parvovirus B19 in human colorectal cancer
Article Snippet: .. The amplification reaction consisted of 10X PCR buffer, 1.25 units of JumpStart™ Taq (Sigma-Aldrich), 10 pmol forward and reverse primers, 0.2 μmol dNTP, 100 ng template and 0.2X SYBR-Green I (Amresco Inc., Solon, OH, USA) in a final volume of 50 μl. .. The reactions were performed using the StepOneTM Real-Time PCR System (Applied Biosystems, Inc., Foster City, CA, USA).

Article Title: Alteration of colonic excitatory tachykininergic motility and enteric inflammation following dopaminergic nigrostriatal neurodegeneration
Article Snippet: RT was performed in a mixture of 2 μL RT buffer, 0.8 μL dNTP, 2 μL random primer, 1 μL reverse transcriptase, and nuclease-free water (Amresco LLC, Solon, USA) up to 10 μL. .. SYBR Green (Applied Biosystems, Thermo Fisher Scientific Inc., CA, USA) qPCR reactions were run in Light Cycler 480 II Real-Time PCR Instrument (Roche, Basel, Switzerland).

RNA Extraction:

Article Title: Alteration of colonic excitatory tachykininergic motility and enteric inflammation following dopaminergic nigrostriatal neurodegeneration
Article Snippet: Paragraph title: RNA extraction and RT-qPCR ... RT was performed in a mixture of 2 μL RT buffer, 0.8 μL dNTP, 2 μL random primer, 1 μL reverse transcriptase, and nuclease-free water (Amresco LLC, Solon, USA) up to 10 μL.

Agarose Gel Electrophoresis:

Article Title: Detection and genotyping of Helicobacter pylori in saliva versus stool samples from asymptomatic individuals in Northeastern Thailand reveals intra-host tissue-specific H. pylori subtypes
Article Snippet: PCR was performed in a total volume of 25 μl containing 500 ng DNA template, 1X PCR buffer (+ 1.5 mM MgCl2 ), 0.4 μM of each primer, 0.5 unit of Taq DNA polymerase (RBC bioscience, Taipei, Taiwan) and 0.2 mM dNTP (Amresco, Ohio, USA) using a thermal cycler (C1000™ Thermal Cycler, BioRad). .. Amplified products were separated by electrophoresis in a 1.5% agarose gel and visualized by staining with ethidium bromide.

Article Title: Detection and genotyping of Helicobacter pylori in saliva versus stool samples from asymptomatic individuals in Northeastern Thailand reveals intra-host tissue-specific H. pylori subtypes
Article Snippet: PCR was performed in a total volume of 25 μl containing 500 ng DNA template, 1X PCR buffer (+ 1.5 mM MgCl2 ), 0.4 μM of each primer, 0.5 unit of Taq DNA polymerase (RBC bioscience, Taipei, Taiwan) and 0.2 mM dNTP (Amresco, Ohio, USA) using a thermal cycler (C1000™ Thermal Cycler, BioRad). .. Amplified products were separated by electrophoresis in a 1.5% agarose gel and visualized by staining with ethidium bromide.

Article Title: Neuroglobin-Deficiency Exacerbates Hif1A and c-FOS Response, but Does Not Affect Neuronal Survival during Severe Hypoxia In Vivo
Article Snippet: Genotyping Tail-clip genotyping was performed using the following primer sets in 0.2 µM concentrations: 5′-CTCTCAGGCGTGAGGTAAGG-3′ , 5′-CTGGCTCCCACTGTATTGGT-3′ , 5′-CCCAGA TTCTGATACCCAC-3′ and 5′-GCACTCCTTGCTGAGACCTAG-3′ using 1× DyNazyme reaction buffer and DyNazyme I polymerase (cat. No. F-500S Finnzymes, Finland), 0.25 µM dNTP (Amresco, USA). .. The PCR products were run on a 1% agarose gel where bands of approximately 2000 bp and 600 bp indicate wt (wt), 600 bp and 180 bp bands indicate heterozygous, and 180 bp band indicates homozygous Ngb-deficient genotype (Ngb-null).

Size-exclusion Chromatography:

Article Title: Neuroglobin-Deficiency Exacerbates Hif1A and c-FOS Response, but Does Not Affect Neuronal Survival during Severe Hypoxia In Vivo
Article Snippet: Genotyping Tail-clip genotyping was performed using the following primer sets in 0.2 µM concentrations: 5′-CTCTCAGGCGTGAGGTAAGG-3′ , 5′-CTGGCTCCCACTGTATTGGT-3′ , 5′-CCCAGA TTCTGATACCCAC-3′ and 5′-GCACTCCTTGCTGAGACCTAG-3′ using 1× DyNazyme reaction buffer and DyNazyme I polymerase (cat. No. F-500S Finnzymes, Finland), 0.25 µM dNTP (Amresco, USA). .. The PCR reaction was run as follows: 94°C 2 min, 92°C 1 min, 35 cycles of 60°C 1 min, 72°C 30 sec, 92°C 1 min, 60°C 1 min, 72°C 10 min.

Spectrophotometry:

Article Title: Alteration of colonic excitatory tachykininergic motility and enteric inflammation following dopaminergic nigrostriatal neurodegeneration
Article Snippet: The concentration of isolated RNA was determined with a spectrophotometer, and 2 μg of RNA were used for the reverse transcription (RT) procedure (High Capacity cDNA Reverse Transcription Kit, Applied Biosystems, Thermo Fisher Scientific Inc., CA, USA). .. RT was performed in a mixture of 2 μL RT buffer, 0.8 μL dNTP, 2 μL random primer, 1 μL reverse transcriptase, and nuclease-free water (Amresco LLC, Solon, USA) up to 10 μL.

Concentration Assay:

Article Title: Alteration of colonic excitatory tachykininergic motility and enteric inflammation following dopaminergic nigrostriatal neurodegeneration
Article Snippet: The concentration of isolated RNA was determined with a spectrophotometer, and 2 μg of RNA were used for the reverse transcription (RT) procedure (High Capacity cDNA Reverse Transcription Kit, Applied Biosystems, Thermo Fisher Scientific Inc., CA, USA). .. RT was performed in a mixture of 2 μL RT buffer, 0.8 μL dNTP, 2 μL random primer, 1 μL reverse transcriptase, and nuclease-free water (Amresco LLC, Solon, USA) up to 10 μL.

CTG Assay:

Article Title: A multi-locus approach to barcoding in the Anopheles strodei subgroup (Diptera: Culicidae)
Article Snippet: This amplification product then served as a template in a sequencing reaction using internal primers W1F (5′-GAT CAA RAA GAT CTG YGA CTC GTT-3′) and W2R (5′GCC ATC GAG ATG GAG GAG CTG-3′). .. Both PCRs were carried out in a 25-μL aqueous reaction mixture containing 1 μl DNA extraction solution, 1X PCR buffer (Invitrogen), 1.5 mM MgCl2 (Invitrogen), 2.5 μL of dimethyl sulfoxide (Sigma), 2.0 μM of each primer, 0.2 mM each dNTP (Amresco) and 2.5 U Taq Platinum polymerase (Invitrogen).

Staining:

Article Title: Detection and genotyping of Helicobacter pylori in saliva versus stool samples from asymptomatic individuals in Northeastern Thailand reveals intra-host tissue-specific H. pylori subtypes
Article Snippet: PCR was performed in a total volume of 25 μl containing 500 ng DNA template, 1X PCR buffer (+ 1.5 mM MgCl2 ), 0.4 μM of each primer, 0.5 unit of Taq DNA polymerase (RBC bioscience, Taipei, Taiwan) and 0.2 mM dNTP (Amresco, Ohio, USA) using a thermal cycler (C1000™ Thermal Cycler, BioRad). .. Amplified products were separated by electrophoresis in a 1.5% agarose gel and visualized by staining with ethidium bromide.

Article Title: Detection and genotyping of Helicobacter pylori in saliva versus stool samples from asymptomatic individuals in Northeastern Thailand reveals intra-host tissue-specific H. pylori subtypes
Article Snippet: PCR was performed in a total volume of 25 μl containing 500 ng DNA template, 1X PCR buffer (+ 1.5 mM MgCl2 ), 0.4 μM of each primer, 0.5 unit of Taq DNA polymerase (RBC bioscience, Taipei, Taiwan) and 0.2 mM dNTP (Amresco, Ohio, USA) using a thermal cycler (C1000™ Thermal Cycler, BioRad). .. Amplified products were separated by electrophoresis in a 1.5% agarose gel and visualized by staining with ethidium bromide.

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    Amresco dntp
    Dntp, supplied by Amresco, used in various techniques. Bioz Stars score: 96/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dntp - by Bioz Stars, 2020-03
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