Structured Review

Amresco dntp
Dntp, supplied by Amresco, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dntp/product/Amresco
Average 92 stars, based on 8 article reviews
Price from $9.99 to $1999.99
dntp - by Bioz Stars, 2020-07
92/100 stars

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Polymerase Chain Reaction:

Article Title: Detection and genotyping of Helicobacter pylori in saliva versus stool samples from asymptomatic individuals in Northeastern Thailand reveals intra-host tissue-specific H. pylori subtypes
Article Snippet: .. PCR was performed in a total volume of 25 μl containing 500 ng DNA template, 1X PCR buffer (+ 1.5 mM MgCl2 ), 0.4 μM of each primer, 0.5 unit of Taq DNA polymerase (RBC bioscience, Taipei, Taiwan) and 0.2 mM dNTP (Amresco, Ohio, USA) using a thermal cycler (C1000™ Thermal Cycler, BioRad). .. PCR conditions and primer sequences are shown in Table .

Article Title: Gene expression analysis of potential genes and pathways involved in the pathogenic mechanisms of parvovirus B19 in human colorectal cancer
Article Snippet: .. The amplification reaction consisted of 10X PCR buffer, 1.25 units of JumpStart™ Taq (Sigma-Aldrich), 10 pmol forward and reverse primers, 0.2 μmol dNTP, 100 ng template and 0.2X SYBR-Green I (Amresco Inc., Solon, OH, USA) in a final volume of 50 μl. .. The reactions were performed using the StepOneTM Real-Time PCR System (Applied Biosystems, Inc., Foster City, CA, USA).

Article Title: Detection and genotyping of Helicobacter pylori in saliva versus stool samples from asymptomatic individuals in Northeastern Thailand reveals intra-host tissue-specific H. pylori subtypes
Article Snippet: .. PCR was performed in a total volume of 25 μl containing 500 ng DNA template, 1X PCR buffer (+ 1.5 mM MgCl2 ), 0.4 μM of each primer, 0.5 unit of Taq DNA polymerase (RBC bioscience, Taipei, Taiwan) and 0.2 mM dNTP (Amresco, Ohio, USA) using a thermal cycler (C1000™ Thermal Cycler, BioRad). .. PCR conditions and primer sequences are shown in Table .

Article Title: A multi-locus approach to barcoding in the Anopheles strodei subgroup (Diptera: Culicidae)
Article Snippet: .. The PCR was carried out in a 25-μL aqueous reaction mixture containing 1 μL of DNA extraction solution, 1X PCR buffer (Invitrogen), 1.5 mM MgCl2 (Invitrogen), 1.25 μL dimethyl sulfoxide (Sigma), 0.1 μM of each primer, 0.2 mM each dNTP (Amresco) and 1.25 U Taq Platinum polymerase (Invitrogen). .. The reaction proceeded under the following temperature profile: 94°C for 2 min, 34 cycles of 94°C for 30 s, 57°C for 30 s and 72°C for 30 s and a final extension at 72°C for 10 min. ITS2 amplicons that yielded ambiguous sequence chromatograms, which is suggestive of intragenomic variation, were purified using PEG precipitation (20% polyethylene glycol 8,000/2.5 M NaCl) and then cloned into pGem-T Easy Vector (Promega, Madison, WI).

Article Title: A multi-locus approach to barcoding in the Anopheles strodei subgroup (Diptera: Culicidae)
Article Snippet: .. Both PCRs were carried out in a 25-μL aqueous reaction mixture containing 1 μl DNA extraction solution, 1X PCR buffer (Invitrogen), 1.5 mM MgCl2 (Invitrogen), 2.5 μL of dimethyl sulfoxide (Sigma), 2.0 μM of each primer, 0.2 mM each dNTP (Amresco) and 2.5 U Taq Platinum polymerase (Invitrogen). .. Both PCRs proceeded under the following temperature profile: 94°C for 5 min, 35 cycles at 94°C for 30 s, an annealing temperature of 50°C for 1 min and then 72°C for 2 min followed by a final extension at 72°C for 10 min. Any white amplicons that yielded ambiguous sequence chromatograms were purified using PEG precipitation (20% polyethylene glycol 8,000/2.5 M NaCl) and then cloned into pGem-T Easy Vector (Promega).

Article Title: A multi-locus approach to barcoding in the Anopheles strodei subgroup (Diptera: Culicidae)
Article Snippet: .. The Polymerase Chain Reaction (PCR) was carried out in a 25-μL aqueous reaction mixture containing 1 μL of DNA extraction solution, 1X PCR buffer (Invitrogen, Carlsbad, CA, USA), 1.5 mM MgCl2 (Invitrogen), 1.25 μL dimethly sulfoxide (Sigma, St. Louis, MO, USA), 0.1 μM of each primer, 0.2 mM each dNTP (Amresco, Solon, OH, USA) and 1.25 U Taq Platinum polymerase (Invitrogen). .. ITS2 region This region was amplified using 5.8SF (5′-ATC ACT CGG CTC GTG GAT CG-3′) and 28SR (5′-ATG CTT AAA TTT AGG GGG TAG TC-3′) primers [ ].

SYBR Green Assay:

Article Title: Gene expression analysis of potential genes and pathways involved in the pathogenic mechanisms of parvovirus B19 in human colorectal cancer
Article Snippet: .. The amplification reaction consisted of 10X PCR buffer, 1.25 units of JumpStart™ Taq (Sigma-Aldrich), 10 pmol forward and reverse primers, 0.2 μmol dNTP, 100 ng template and 0.2X SYBR-Green I (Amresco Inc., Solon, OH, USA) in a final volume of 50 μl. .. The reactions were performed using the StepOneTM Real-Time PCR System (Applied Biosystems, Inc., Foster City, CA, USA).

Amplification:

Article Title: Gene expression analysis of potential genes and pathways involved in the pathogenic mechanisms of parvovirus B19 in human colorectal cancer
Article Snippet: .. The amplification reaction consisted of 10X PCR buffer, 1.25 units of JumpStart™ Taq (Sigma-Aldrich), 10 pmol forward and reverse primers, 0.2 μmol dNTP, 100 ng template and 0.2X SYBR-Green I (Amresco Inc., Solon, OH, USA) in a final volume of 50 μl. .. The reactions were performed using the StepOneTM Real-Time PCR System (Applied Biosystems, Inc., Foster City, CA, USA).

DNA Extraction:

Article Title: A multi-locus approach to barcoding in the Anopheles strodei subgroup (Diptera: Culicidae)
Article Snippet: .. The PCR was carried out in a 25-μL aqueous reaction mixture containing 1 μL of DNA extraction solution, 1X PCR buffer (Invitrogen), 1.5 mM MgCl2 (Invitrogen), 1.25 μL dimethyl sulfoxide (Sigma), 0.1 μM of each primer, 0.2 mM each dNTP (Amresco) and 1.25 U Taq Platinum polymerase (Invitrogen). .. The reaction proceeded under the following temperature profile: 94°C for 2 min, 34 cycles of 94°C for 30 s, 57°C for 30 s and 72°C for 30 s and a final extension at 72°C for 10 min. ITS2 amplicons that yielded ambiguous sequence chromatograms, which is suggestive of intragenomic variation, were purified using PEG precipitation (20% polyethylene glycol 8,000/2.5 M NaCl) and then cloned into pGem-T Easy Vector (Promega, Madison, WI).

Article Title: A multi-locus approach to barcoding in the Anopheles strodei subgroup (Diptera: Culicidae)
Article Snippet: .. Both PCRs were carried out in a 25-μL aqueous reaction mixture containing 1 μl DNA extraction solution, 1X PCR buffer (Invitrogen), 1.5 mM MgCl2 (Invitrogen), 2.5 μL of dimethyl sulfoxide (Sigma), 2.0 μM of each primer, 0.2 mM each dNTP (Amresco) and 2.5 U Taq Platinum polymerase (Invitrogen). .. Both PCRs proceeded under the following temperature profile: 94°C for 5 min, 35 cycles at 94°C for 30 s, an annealing temperature of 50°C for 1 min and then 72°C for 2 min followed by a final extension at 72°C for 10 min. Any white amplicons that yielded ambiguous sequence chromatograms were purified using PEG precipitation (20% polyethylene glycol 8,000/2.5 M NaCl) and then cloned into pGem-T Easy Vector (Promega).

Article Title: A multi-locus approach to barcoding in the Anopheles strodei subgroup (Diptera: Culicidae)
Article Snippet: .. The Polymerase Chain Reaction (PCR) was carried out in a 25-μL aqueous reaction mixture containing 1 μL of DNA extraction solution, 1X PCR buffer (Invitrogen, Carlsbad, CA, USA), 1.5 mM MgCl2 (Invitrogen), 1.25 μL dimethly sulfoxide (Sigma, St. Louis, MO, USA), 0.1 μM of each primer, 0.2 mM each dNTP (Amresco, Solon, OH, USA) and 1.25 U Taq Platinum polymerase (Invitrogen). .. ITS2 region This region was amplified using 5.8SF (5′-ATC ACT CGG CTC GTG GAT CG-3′) and 28SR (5′-ATG CTT AAA TTT AGG GGG TAG TC-3′) primers [ ].

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    Amresco dntp
    Dntp, supplied by Amresco, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dntp/product/Amresco
    Average 92 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    dntp - by Bioz Stars, 2020-07
    92/100 stars
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