dntp mixtures  (Bio-Rad)

 
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    Name:
    dNTP Mix
    Description:
    200 μl premixed dNTP solution 10 mM each dATP dCTP dGTP dTTP education use only
    Catalog Number:
    1708874EDU
    Price:
    None
    Category:
    PCR Instrumentation
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    Structured Review

    Bio-Rad dntp mixtures
    dNTP Mix
    200 μl premixed dNTP solution 10 mM each dATP dCTP dGTP dTTP education use only
    https://www.bioz.com/result/dntp mixtures/product/Bio-Rad
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dntp mixtures - by Bioz Stars, 2020-08
    94/100 stars

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    Related Articles

    Amplification:

    Article Title: Catalase overexpression modulates metabolic parameters in a new ‘stress-less’ leptin-deficient mouse model
    Article Snippet: .. For genotyping, 0.25 μg (1 μL) of each DNA sample was added to a reaction mix of 18.125 μL RNase free H2 O, 2.5 μL of 10X i Taq Buffer, 0.75 μL of MgCl2 50 mM, 0.5 μL dNTP mix 10 mM, 1 μL forward primer, 1 μL reverse primer, 0.125 μL i Taq DNA polymerase to prepare for amplification of DNA in the BioRad MyiQ (BioRad, Hercules, CA). .. PCR protocol was conducted as described in previous publications [ , ].

    Article Title: Optimization of DNA extraction for advancing coral microbiota investigations
    Article Snippet: .. To assess PCR efficiency, unaltered DNA template (0.18–47 ng μl−1 ) was amplified in 25 μl reactions containing 1.25 units of GoTaq® Flexi DNA Polymerase (Promega), 5× Colorless GoTaq® Flexi Buffer, 2.5 mM MgCl2 , 200 μM dNTP mix, and 200 nM of each barcoded primer in a thermocycler (Bio-Rad Laboratories). .. The following PCR reaction conditions were used: 95 °C for 2 min, followed by 40 cycles of 95 °C for 20 s, 55 °C for 15 s, and 72 °C for 5 min, concluding with an extension step of 72 °C for 10 min. PCR products were visually screened electrophoretically for quality using a 1% agarose and tris-borate-EDTA gel illuminated with ultraviolet light with the Hyperladder 50 bp DNA ladder (5 ng μl−1 ) (Bioline).

    Polymerase Chain Reaction:

    Article Title: CCAAT-enhancer binding protein-α (C/EBPα) and hepatocyte nuclear factor 4α (HNF4α) regulate expression of the human fructose-1,6-bisphosphatase 1 (FBP1) gene in human hepatocellular carcinoma HepG2 cells
    Article Snippet: .. The PCR was carried out in a 50 μl reaction mixture containing 1x PCR reaction buffer (20 mM Tris-HCl pH 8.4, 50 mM KCl), 0.2 mM dNTP mixture, 1.5 mM MgCl2 , 0.5 μM each primer, 50 ng genomic DNA and 2.5 units Taq DNA polymerase in an automated thermal cycler MJ Mini (Bio-Rad). .. The PCR profile consisted of initial denaturation at 94°C for 5 min followed by 30 cycles of denaturation at 94°C for 45 sec, annealing at 60°C for 45 sec and extension at 72°C for 2 min, and final extension at 72°C for 10 min.

    Article Title: Optimization of DNA extraction for advancing coral microbiota investigations
    Article Snippet: .. To assess PCR efficiency, unaltered DNA template (0.18–47 ng μl−1 ) was amplified in 25 μl reactions containing 1.25 units of GoTaq® Flexi DNA Polymerase (Promega), 5× Colorless GoTaq® Flexi Buffer, 2.5 mM MgCl2 , 200 μM dNTP mix, and 200 nM of each barcoded primer in a thermocycler (Bio-Rad Laboratories). .. The following PCR reaction conditions were used: 95 °C for 2 min, followed by 40 cycles of 95 °C for 20 s, 55 °C for 15 s, and 72 °C for 5 min, concluding with an extension step of 72 °C for 10 min. PCR products were visually screened electrophoretically for quality using a 1% agarose and tris-borate-EDTA gel illuminated with ultraviolet light with the Hyperladder 50 bp DNA ladder (5 ng μl−1 ) (Bioline).

    Incubation:

    Article Title: High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity
    Article Snippet: .. 100 pmol off-target (#) fwd and off-target (#) rev primers were incubated at 95 °C and cooled at 0.1 °C/s to 37 °C in NEBuffer2 (50 mM sodium chloride, 10 mM Tris-HCl, 10 mM magnesium chloride, 1 mM dithiothreitol, pH 7.9) supplemented with 10 μM dNTP mix (Bio-Rad). .. 10 U of Klenow Fragment (3′→5′ exo-) (NEB) were added to the reaction mixture and double-stranded off-target templates were obtained by overlap extension for 1 h at 37 °C followed by enzyme inactivation for 20 min at 75 °C, then purified with the QIAquick PCR Purification Kit (Qiagen).

    Polyacrylamide Gel Electrophoresis:

    Article Title: In Vitro Selection Using Modified or Unnatural Nucleotides
    Article Snippet: .. Transcribed RNA pools, modified and unmodified (prepared as in Basic Protocol 1 but without radiolabeled ATP) 3000 Ci/mmol α-32 P-labeled dATP (e.g., Perkin Elmer) ThermoScript reverse transcriptase kit (Invitrogen, Life Technologies), kit includes: 5× Buffer, 100 mM DTT, RNase OUT (ribonuclease inhibitor), and RT enzyme 100 µM reverse (3’-end) primer 10 mM dNTP mix (containing 10 mM each of dATP, dCTP, dGTP, and dTTP) 2× denaturing stop dye (see recipe) 10% (w/v) denaturing polyacrylamide gel (see recipe; APPENDIX 3B ), 0.8 mm thick Thermal cycler Gel blotting paper (Bio-Rad) Plastic wrap Gel dryer with heat and vacuum (e.g., Bio-Rad) Phosphorimager screen Phosphorimager Image analysis software (e.g., GE Healthcare Life Sciences ImageQuant) Additional reagents and equipment for denaturing polyacrylamide gel electrophoresis (PAGE; APPENDIX 3B ) .. 1 Combine the following RT reactions in two separate tubes (13 µL total volume): 10.5 µL (10 pmol or ~0.5 µg) transcribed RNA pool (modified in one tube, unmodified in the other) 1.0 µL 100 µM reverse (3’-end) primer 0.5 µL α-32 P-labeled dATP (this volume should be varied based on the age and subsequent activity of the radiolabeled dATP) 1.0 µL 10 mM dNTP mix Do not use radiolabeled RNA as the input.

    Modification:

    Article Title: In Vitro Selection Using Modified or Unnatural Nucleotides
    Article Snippet: .. Transcribed RNA pools, modified and unmodified (prepared as in Basic Protocol 1 but without radiolabeled ATP) 3000 Ci/mmol α-32 P-labeled dATP (e.g., Perkin Elmer) ThermoScript reverse transcriptase kit (Invitrogen, Life Technologies), kit includes: 5× Buffer, 100 mM DTT, RNase OUT (ribonuclease inhibitor), and RT enzyme 100 µM reverse (3’-end) primer 10 mM dNTP mix (containing 10 mM each of dATP, dCTP, dGTP, and dTTP) 2× denaturing stop dye (see recipe) 10% (w/v) denaturing polyacrylamide gel (see recipe; APPENDIX 3B ), 0.8 mm thick Thermal cycler Gel blotting paper (Bio-Rad) Plastic wrap Gel dryer with heat and vacuum (e.g., Bio-Rad) Phosphorimager screen Phosphorimager Image analysis software (e.g., GE Healthcare Life Sciences ImageQuant) Additional reagents and equipment for denaturing polyacrylamide gel electrophoresis (PAGE; APPENDIX 3B ) .. 1 Combine the following RT reactions in two separate tubes (13 µL total volume): 10.5 µL (10 pmol or ~0.5 µg) transcribed RNA pool (modified in one tube, unmodified in the other) 1.0 µL 100 µM reverse (3’-end) primer 0.5 µL α-32 P-labeled dATP (this volume should be varied based on the age and subsequent activity of the radiolabeled dATP) 1.0 µL 10 mM dNTP mix Do not use radiolabeled RNA as the input.

    Software:

    Article Title: In Vitro Selection Using Modified or Unnatural Nucleotides
    Article Snippet: .. Transcribed RNA pools, modified and unmodified (prepared as in Basic Protocol 1 but without radiolabeled ATP) 3000 Ci/mmol α-32 P-labeled dATP (e.g., Perkin Elmer) ThermoScript reverse transcriptase kit (Invitrogen, Life Technologies), kit includes: 5× Buffer, 100 mM DTT, RNase OUT (ribonuclease inhibitor), and RT enzyme 100 µM reverse (3’-end) primer 10 mM dNTP mix (containing 10 mM each of dATP, dCTP, dGTP, and dTTP) 2× denaturing stop dye (see recipe) 10% (w/v) denaturing polyacrylamide gel (see recipe; APPENDIX 3B ), 0.8 mm thick Thermal cycler Gel blotting paper (Bio-Rad) Plastic wrap Gel dryer with heat and vacuum (e.g., Bio-Rad) Phosphorimager screen Phosphorimager Image analysis software (e.g., GE Healthcare Life Sciences ImageQuant) Additional reagents and equipment for denaturing polyacrylamide gel electrophoresis (PAGE; APPENDIX 3B ) .. 1 Combine the following RT reactions in two separate tubes (13 µL total volume): 10.5 µL (10 pmol or ~0.5 µg) transcribed RNA pool (modified in one tube, unmodified in the other) 1.0 µL 100 µM reverse (3’-end) primer 0.5 µL α-32 P-labeled dATP (this volume should be varied based on the age and subsequent activity of the radiolabeled dATP) 1.0 µL 10 mM dNTP mix Do not use radiolabeled RNA as the input.

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  • 92
    Bio-Rad m dntp
    M Dntp, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dntp/product/Bio-Rad
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m dntp - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

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