dntp mix  (Thermo Fisher)


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    Name:
    dNTP Mix
    Description:
    This dNTP mix consists of four nucleotides dATP dCTP dGTP dTTP 2 deoxynucleoside 5 triphosphates each at a concentration of 10 mM in a solution of 0 6 mM Tris HCl pH 7 5 This mix is suitable for use in PCR sequencing fill in nick translation cDNA synthesis TdT tailing reactions and dilution of radiolabeled dNTPs
    Catalog Number:
    18427013
    Price:
    None
    Applications:
    PCR|PCR & Real-Time PCR|PCR Buffers, dNTPs, MgCl2 & General Reagents
    Category:
    Oligos Primers Probes Nucleotides
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    Structured Review

    Thermo Fisher dntp mix
    Vpx+ virus, dN and <t>dNTP</t> treatments result in minor increases in the dNTP pool of HSPCs. MDMs (A, C and E) and freshly isolated HSPCs (B, D and F) were pretreated for 2 h with Vpx+/− viruses, dNs or <t>dNTPs,</t> and cultured for 3 days before cell lysis. Quantification of dNTPs was done by a single nucleotide incorporation assay (see “Material and methods” section). Fold induction was calculated based on the absolute values of vero- vs mock-treated samples (C–F). White dots represent Vpx− virus-pretreated samples, black dots represent Vpx+ virus-pretreated samples (A–B). White bars represent dN-pretreated samples, black bars represent dNTP-pretreated samples (C–F). Data are from three donors and bars represent mean ± SEM. Statistical analysis was done using two-tailed paired t-test.
    This dNTP mix consists of four nucleotides dATP dCTP dGTP dTTP 2 deoxynucleoside 5 triphosphates each at a concentration of 10 mM in a solution of 0 6 mM Tris HCl pH 7 5 This mix is suitable for use in PCR sequencing fill in nick translation cDNA synthesis TdT tailing reactions and dilution of radiolabeled dNTPs
    https://www.bioz.com/result/dntp mix/product/Thermo Fisher
    Average 99 stars, based on 1903 article reviews
    Price from $9.99 to $1999.99
    dntp mix - by Bioz Stars, 2020-10
    99/100 stars

    Images

    1) Product Images from "Vpx mediated degradation of SAMHD1 has only a very limited effect on lentiviral transduction rate in ex vivo cultured HSPCs"

    Article Title: Vpx mediated degradation of SAMHD1 has only a very limited effect on lentiviral transduction rate in ex vivo cultured HSPCs

    Journal: Stem cell research

    doi: 10.1016/j.scr.2015.06.012

    Vpx+ virus, dN and dNTP treatments result in minor increases in the dNTP pool of HSPCs. MDMs (A, C and E) and freshly isolated HSPCs (B, D and F) were pretreated for 2 h with Vpx+/− viruses, dNs or dNTPs, and cultured for 3 days before cell lysis. Quantification of dNTPs was done by a single nucleotide incorporation assay (see “Material and methods” section). Fold induction was calculated based on the absolute values of vero- vs mock-treated samples (C–F). White dots represent Vpx− virus-pretreated samples, black dots represent Vpx+ virus-pretreated samples (A–B). White bars represent dN-pretreated samples, black bars represent dNTP-pretreated samples (C–F). Data are from three donors and bars represent mean ± SEM. Statistical analysis was done using two-tailed paired t-test.
    Figure Legend Snippet: Vpx+ virus, dN and dNTP treatments result in minor increases in the dNTP pool of HSPCs. MDMs (A, C and E) and freshly isolated HSPCs (B, D and F) were pretreated for 2 h with Vpx+/− viruses, dNs or dNTPs, and cultured for 3 days before cell lysis. Quantification of dNTPs was done by a single nucleotide incorporation assay (see “Material and methods” section). Fold induction was calculated based on the absolute values of vero- vs mock-treated samples (C–F). White dots represent Vpx− virus-pretreated samples, black dots represent Vpx+ virus-pretreated samples (A–B). White bars represent dN-pretreated samples, black bars represent dNTP-pretreated samples (C–F). Data are from three donors and bars represent mean ± SEM. Statistical analysis was done using two-tailed paired t-test.

    Techniques Used: Isolation, Cell Culture, Lysis, Two Tailed Test

    Related Articles

    Transduction:

    Article Title: Vpx mediated degradation of SAMHD1 has only a very limited effect on lentiviral transduction rate in ex vivo cultured HSPCs
    Article Snippet: .. THP-1 cells transduced with lentiviral vectors encoding SAMHD1 shRNA were selected by culturing in a medium containing 0.8 μg/ml puromycin. dNs consisted of a mixture of dA (D8668), dC (D0776), dG (D0901) and dT (T1895; all from Sigma-Aldrich). dNTPs (R0192) were purchased from Fermentas (Glen Burnie, MD). .. Cytokine cocktail for HSPC transduction consisted of a mixture of stem cell factor (SCF) at 100 ng/ml, Fms-related tyrosine kinase 3 ligand (Flt3L) at 100 ng/ml, interleukin-3 (IL-3) at 60 ng/ml and thrombopoietin (TPO) at 10 ng/ml (Immunotools, Friesoythe, Germany).

    Amplification:

    Article Title: rpoB Gene Analysis as a Novel Strategy for Identification of Spirochetes from the Genera Borrelia, Treponema, and Leptospira
    Article Snippet: .. Amplification of rpoB fragments was carried out by using the LTB primers listed in Table (3.2 pmol each) added to a 50-μl PCR mix containing 10 mM dNTP, 1 U of Taq polymerase (Gibco BRL), and 2 μl of DNA extract. ..

    Article Title: Reconstituting ParA/ParB-mediated transport of DNA cargo
    Article Snippet: .. pBR322:: sopC plasmid (PCR template) PCR Primers for sopC DNA amplification: (Primers synthesized by IDT) 10 mM dNTPs (Life Technologies; Cat. # 18427–013) 100 mM MgSO4 (New England Biolabs, Cat. # M0254S) 2000 U/mL VENT Polymerase (New England Biolabs, Cat. # M0254S) SYBR Gold DNA Stain (Life Technologies, Cat. # S-11494) Illustra MicroSpin S-400HR columns (GE Healthcare, Cat # 27–5140-01) MyOne Streptavidin C1 Dynabeads (Life Technologies, Cat. # 65001) 20 U/μL PstI Restriction Enzyme (New England Biolabs, Cat. # R0140S) Phenol:CHCl3:Isoamyl Alcohol (UltraPure 25:24:1 v/v) Ethanol. .. PCR Thermocycler Tabletop centrifuge Tube rotator BioMag Separator (Advanced Magnetics Inc.) Nanodrop Spectrophotometer.

    Article Title: Significance of UGT1A1*28 Genotype in Patients with Advanced Liver Injury Caused By Chronic Hepatitis C
    Article Snippet: .. The amplification reaction was performed in a total volume of 25 μL, and the reaction mix contained 20 pmol of each primer, 50–100 ng of genomic DNA, 200 mmol/L of each dNTP (Fermentas, ON, Canada), 1× PCR reaction buffer (Qiagen), 1× Q solution (Qiagen), 2.75 mmol/L MgCl2 , 1 U HotStar DNA polymerase (Qiagen). .. The temperature profile of the PCR reactions was for the initial activation of DNA polymerase set at 95 °C for 15 min, followed by 35 cycles of 30 sec denaturation at 95 °C, 30 sec annealing at 63 °C, and 30 sec elongation at 72 °C, ended by a final extension period of 7 min at 72 °C.

    Synthesized:

    Article Title: Reconstituting ParA/ParB-mediated transport of DNA cargo
    Article Snippet: .. pBR322:: sopC plasmid (PCR template) PCR Primers for sopC DNA amplification: (Primers synthesized by IDT) 10 mM dNTPs (Life Technologies; Cat. # 18427–013) 100 mM MgSO4 (New England Biolabs, Cat. # M0254S) 2000 U/mL VENT Polymerase (New England Biolabs, Cat. # M0254S) SYBR Gold DNA Stain (Life Technologies, Cat. # S-11494) Illustra MicroSpin S-400HR columns (GE Healthcare, Cat # 27–5140-01) MyOne Streptavidin C1 Dynabeads (Life Technologies, Cat. # 65001) 20 U/μL PstI Restriction Enzyme (New England Biolabs, Cat. # R0140S) Phenol:CHCl3:Isoamyl Alcohol (UltraPure 25:24:1 v/v) Ethanol. .. PCR Thermocycler Tabletop centrifuge Tube rotator BioMag Separator (Advanced Magnetics Inc.) Nanodrop Spectrophotometer.

    Protease Inhibitor:

    Article Title: Whole-genome chromatin profiling from limited numbers of cells using nano-ChIP-seq
    Article Snippet: .. 25530-031) Protease inhibitor cocktail (Roche, Cat.#: 11836153001): Phenol:Chloroform:Isoamyl Alchohol (Invitrogen, Cat. #:15593031) RNAse (Invitrogen, Cat. #:12091-021) Sequenase Enzyme Kit (Version 2.0, US Biochemical, Cat. #: 70775 dNTP mix (Invitrogen, Cat.#: 18427-013 ) BSA (10 mg/ml , NEB, #B9001S) DTT (0.1 M stock, Invitrogen, #:Y00147) Primer 1 (4 μM): 5'-GACATGTATCCGGATGTNNNNNNNNN-3' ExoSAP-IT (USB, Cat.#:78200) Phusion Polymerase (NEB, Cat#: F-530L, provided with 100 % DMSO, 5X GC buffer) Primer 2 (10 μM stock): 5'-GACATGTATCCGGATGT-3’ BciVI Restriction Enzyme (NEB, Cat. #: R0596L) Agarose (Fisher, Cat #: BP160-500) Protein A-sepharose beads (Sigma, Cat#: P9424) PCR purification, Gel extraction and reaction clean up kits (Qiagen, Cat#s: 28006, 28606, 28206) Glycogen (20μg/μl, Invitrogen, Cat#: 10814-010) Quant-It dsDNA HS assay kit (Invitrogen, Cat#: ) .. 0.2 ml PCR tube strips (Eppendorf, Cat. #: 951010022) 1.5 ml tubes low retention tubes (Eppendorf, Cat. #:022431021) 15 ml tubes (BD Falcon cat. no. 352097) Filtered micropipette tips (Danville Sci., Cat.#s:P1096FR, P1121, P1122, P1126 ) Micropipiettes (1-1000 μl range) Spin-X wash columns (Costar, Cat#:8160) Refrigerated microcentrifuge (Eppendorf model 5415R or equivalent) Minicentrifuge with strip tubes adapter (Fisher Scientific Cat. #:05090-100) Cold room Sonicator (Branson 250 Digitial sonifier) Rotating mixer for 1.5 ml tubes Vortex mixer Thermal Cycler (Eppendorf, master cycle gradient or equivalent) Agarose gel electrophoresis equipment Qubit Flourometer (Invitrogen, Cat #: 32857)

    Purification:

    Article Title: Whole-genome chromatin profiling from limited numbers of cells using nano-ChIP-seq
    Article Snippet: .. 25530-031) Protease inhibitor cocktail (Roche, Cat.#: 11836153001): Phenol:Chloroform:Isoamyl Alchohol (Invitrogen, Cat. #:15593031) RNAse (Invitrogen, Cat. #:12091-021) Sequenase Enzyme Kit (Version 2.0, US Biochemical, Cat. #: 70775 dNTP mix (Invitrogen, Cat.#: 18427-013 ) BSA (10 mg/ml , NEB, #B9001S) DTT (0.1 M stock, Invitrogen, #:Y00147) Primer 1 (4 μM): 5'-GACATGTATCCGGATGTNNNNNNNNN-3' ExoSAP-IT (USB, Cat.#:78200) Phusion Polymerase (NEB, Cat#: F-530L, provided with 100 % DMSO, 5X GC buffer) Primer 2 (10 μM stock): 5'-GACATGTATCCGGATGT-3’ BciVI Restriction Enzyme (NEB, Cat. #: R0596L) Agarose (Fisher, Cat #: BP160-500) Protein A-sepharose beads (Sigma, Cat#: P9424) PCR purification, Gel extraction and reaction clean up kits (Qiagen, Cat#s: 28006, 28606, 28206) Glycogen (20μg/μl, Invitrogen, Cat#: 10814-010) Quant-It dsDNA HS assay kit (Invitrogen, Cat#: ) .. 0.2 ml PCR tube strips (Eppendorf, Cat. #: 951010022) 1.5 ml tubes low retention tubes (Eppendorf, Cat. #:022431021) 15 ml tubes (BD Falcon cat. no. 352097) Filtered micropipette tips (Danville Sci., Cat.#s:P1096FR, P1121, P1122, P1126 ) Micropipiettes (1-1000 μl range) Spin-X wash columns (Costar, Cat#:8160) Refrigerated microcentrifuge (Eppendorf model 5415R or equivalent) Minicentrifuge with strip tubes adapter (Fisher Scientific Cat. #:05090-100) Cold room Sonicator (Branson 250 Digitial sonifier) Rotating mixer for 1.5 ml tubes Vortex mixer Thermal Cycler (Eppendorf, master cycle gradient or equivalent) Agarose gel electrophoresis equipment Qubit Flourometer (Invitrogen, Cat #: 32857)

    shRNA:

    Article Title: Vpx mediated degradation of SAMHD1 has only a very limited effect on lentiviral transduction rate in ex vivo cultured HSPCs
    Article Snippet: .. THP-1 cells transduced with lentiviral vectors encoding SAMHD1 shRNA were selected by culturing in a medium containing 0.8 μg/ml puromycin. dNs consisted of a mixture of dA (D8668), dC (D0776), dG (D0901) and dT (T1895; all from Sigma-Aldrich). dNTPs (R0192) were purchased from Fermentas (Glen Burnie, MD). .. Cytokine cocktail for HSPC transduction consisted of a mixture of stem cell factor (SCF) at 100 ng/ml, Fms-related tyrosine kinase 3 ligand (Flt3L) at 100 ng/ml, interleukin-3 (IL-3) at 60 ng/ml and thrombopoietin (TPO) at 10 ng/ml (Immunotools, Friesoythe, Germany).

    Lysis:

    Article Title: Direct Cell Lysis for Single-Cell Gene Expression Profiling
    Article Snippet: .. The following chemicals were evaluated (final lysis concentrations are shown): 7-deaza-2′-deoxyguanosine-5′-triphosphate lithium salt (100 μM, Sigma-Aldrich); Betaine solution (4 M, Sigma-Aldrich); BSA (1–4 mg/ml, Fermentas); guanidine thiocyanate solution (GTC) (40–80 mM, Sigma-Aldrich); GenElute linear polyacrylamide (LPA) (50 ng/μl, Sigma-Aldrich); Igepal CA-630 (also known as Non-idet P-40, 0.5–4%, Sigma-Aldrich); polyinosinic acid potassium salt (50 ng/μl, Sigma-Aldrich); RNAseOUT (10 U/μl, Invitrogen); 2× reverse transcription buffer: 100 mM Tris-HCl (pH 8.3), 150 mM KCl, and 6 mM MgCl2 (Invitrogen); d -(+)-trehalose dihydrate (1 M, Sigma-Aldrich); yeast tRNA (50 ng/μl, Ambion); RT mix (2× RT buffer, Invitrogen, 5 μM random hexamers (Metabion), 5 μM oligo-dT (Metabion), 1 mM dNTP); RT mix with BSA (2× RT buffer, 5 μM random hexamers, 5 μM oligo-dT, 1 mM dNTP, 1 mg/ml BSA); and RNase-free water (Gibco). ..

    Polymerase Chain Reaction:

    Article Title: A mutant Pfu DNA polymerase designed for advanced uracil-excision DNA engineering
    Article Snippet: .. PCR with dUTP replacing dTTP was performed using standard Phusion (Finnzymes) reaction conditions as recommended by the manufacturer, using the dUTP/dNTPs mix (Fermentas) instead of dNTPs. .. The pET-Pfu-vector was a kind gift from Jens Lykke-Andersen.

    Article Title: Whole-genome chromatin profiling from limited numbers of cells using nano-ChIP-seq
    Article Snippet: .. 25530-031) Protease inhibitor cocktail (Roche, Cat.#: 11836153001): Phenol:Chloroform:Isoamyl Alchohol (Invitrogen, Cat. #:15593031) RNAse (Invitrogen, Cat. #:12091-021) Sequenase Enzyme Kit (Version 2.0, US Biochemical, Cat. #: 70775 dNTP mix (Invitrogen, Cat.#: 18427-013 ) BSA (10 mg/ml , NEB, #B9001S) DTT (0.1 M stock, Invitrogen, #:Y00147) Primer 1 (4 μM): 5'-GACATGTATCCGGATGTNNNNNNNNN-3' ExoSAP-IT (USB, Cat.#:78200) Phusion Polymerase (NEB, Cat#: F-530L, provided with 100 % DMSO, 5X GC buffer) Primer 2 (10 μM stock): 5'-GACATGTATCCGGATGT-3’ BciVI Restriction Enzyme (NEB, Cat. #: R0596L) Agarose (Fisher, Cat #: BP160-500) Protein A-sepharose beads (Sigma, Cat#: P9424) PCR purification, Gel extraction and reaction clean up kits (Qiagen, Cat#s: 28006, 28606, 28206) Glycogen (20μg/μl, Invitrogen, Cat#: 10814-010) Quant-It dsDNA HS assay kit (Invitrogen, Cat#: ) .. 0.2 ml PCR tube strips (Eppendorf, Cat. #: 951010022) 1.5 ml tubes low retention tubes (Eppendorf, Cat. #:022431021) 15 ml tubes (BD Falcon cat. no. 352097) Filtered micropipette tips (Danville Sci., Cat.#s:P1096FR, P1121, P1122, P1126 ) Micropipiettes (1-1000 μl range) Spin-X wash columns (Costar, Cat#:8160) Refrigerated microcentrifuge (Eppendorf model 5415R or equivalent) Minicentrifuge with strip tubes adapter (Fisher Scientific Cat. #:05090-100) Cold room Sonicator (Branson 250 Digitial sonifier) Rotating mixer for 1.5 ml tubes Vortex mixer Thermal Cycler (Eppendorf, master cycle gradient or equivalent) Agarose gel electrophoresis equipment Qubit Flourometer (Invitrogen, Cat #: 32857)

    Article Title: rpoB Gene Analysis as a Novel Strategy for Identification of Spirochetes from the Genera Borrelia, Treponema, and Leptospira
    Article Snippet: .. Amplification of rpoB fragments was carried out by using the LTB primers listed in Table (3.2 pmol each) added to a 50-μl PCR mix containing 10 mM dNTP, 1 U of Taq polymerase (Gibco BRL), and 2 μl of DNA extract. ..

    Article Title: Reconstituting ParA/ParB-mediated transport of DNA cargo
    Article Snippet: .. pBR322:: sopC plasmid (PCR template) PCR Primers for sopC DNA amplification: (Primers synthesized by IDT) 10 mM dNTPs (Life Technologies; Cat. # 18427–013) 100 mM MgSO4 (New England Biolabs, Cat. # M0254S) 2000 U/mL VENT Polymerase (New England Biolabs, Cat. # M0254S) SYBR Gold DNA Stain (Life Technologies, Cat. # S-11494) Illustra MicroSpin S-400HR columns (GE Healthcare, Cat # 27–5140-01) MyOne Streptavidin C1 Dynabeads (Life Technologies, Cat. # 65001) 20 U/μL PstI Restriction Enzyme (New England Biolabs, Cat. # R0140S) Phenol:CHCl3:Isoamyl Alcohol (UltraPure 25:24:1 v/v) Ethanol. .. PCR Thermocycler Tabletop centrifuge Tube rotator BioMag Separator (Advanced Magnetics Inc.) Nanodrop Spectrophotometer.

    Article Title: Significance of UGT1A1*28 Genotype in Patients with Advanced Liver Injury Caused By Chronic Hepatitis C
    Article Snippet: .. The amplification reaction was performed in a total volume of 25 μL, and the reaction mix contained 20 pmol of each primer, 50–100 ng of genomic DNA, 200 mmol/L of each dNTP (Fermentas, ON, Canada), 1× PCR reaction buffer (Qiagen), 1× Q solution (Qiagen), 2.75 mmol/L MgCl2 , 1 U HotStar DNA polymerase (Qiagen). .. The temperature profile of the PCR reactions was for the initial activation of DNA polymerase set at 95 °C for 15 min, followed by 35 cycles of 30 sec denaturation at 95 °C, 30 sec annealing at 63 °C, and 30 sec elongation at 72 °C, ended by a final extension period of 7 min at 72 °C.

    Article Title: Analysis of Endocannabinoid System in Rat Testis During the First Spermatogenetic Wave
    Article Snippet: .. PCR was carried out using 2 µl cDNA and 10 pmol of the appropriate primers in a PCR mix [0.2 mM dNTP, 1× PCR buffer (Invitrogen Life Technologies), 1.5 mM MgCl2 , 1.25 U Taq Polymerase (Invitrogen Life Technologies, Paisley, UK)], using an Applied Biosystem Thermocycler. .. Possible residual genomic DNA was evaluated using Actin primers by RT− cDNA amplification (at 35 circles) while possible contamination among samples was excluded using samples prepared with 1 µl water (H2 O samples, negative control) in place of cDNA.

    Gel Extraction:

    Article Title: Whole-genome chromatin profiling from limited numbers of cells using nano-ChIP-seq
    Article Snippet: .. 25530-031) Protease inhibitor cocktail (Roche, Cat.#: 11836153001): Phenol:Chloroform:Isoamyl Alchohol (Invitrogen, Cat. #:15593031) RNAse (Invitrogen, Cat. #:12091-021) Sequenase Enzyme Kit (Version 2.0, US Biochemical, Cat. #: 70775 dNTP mix (Invitrogen, Cat.#: 18427-013 ) BSA (10 mg/ml , NEB, #B9001S) DTT (0.1 M stock, Invitrogen, #:Y00147) Primer 1 (4 μM): 5'-GACATGTATCCGGATGTNNNNNNNNN-3' ExoSAP-IT (USB, Cat.#:78200) Phusion Polymerase (NEB, Cat#: F-530L, provided with 100 % DMSO, 5X GC buffer) Primer 2 (10 μM stock): 5'-GACATGTATCCGGATGT-3’ BciVI Restriction Enzyme (NEB, Cat. #: R0596L) Agarose (Fisher, Cat #: BP160-500) Protein A-sepharose beads (Sigma, Cat#: P9424) PCR purification, Gel extraction and reaction clean up kits (Qiagen, Cat#s: 28006, 28606, 28206) Glycogen (20μg/μl, Invitrogen, Cat#: 10814-010) Quant-It dsDNA HS assay kit (Invitrogen, Cat#: ) .. 0.2 ml PCR tube strips (Eppendorf, Cat. #: 951010022) 1.5 ml tubes low retention tubes (Eppendorf, Cat. #:022431021) 15 ml tubes (BD Falcon cat. no. 352097) Filtered micropipette tips (Danville Sci., Cat.#s:P1096FR, P1121, P1122, P1126 ) Micropipiettes (1-1000 μl range) Spin-X wash columns (Costar, Cat#:8160) Refrigerated microcentrifuge (Eppendorf model 5415R or equivalent) Minicentrifuge with strip tubes adapter (Fisher Scientific Cat. #:05090-100) Cold room Sonicator (Branson 250 Digitial sonifier) Rotating mixer for 1.5 ml tubes Vortex mixer Thermal Cycler (Eppendorf, master cycle gradient or equivalent) Agarose gel electrophoresis equipment Qubit Flourometer (Invitrogen, Cat #: 32857)

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Whole-genome chromatin profiling from limited numbers of cells using nano-ChIP-seq
    Article Snippet: .. 25530-031) Protease inhibitor cocktail (Roche, Cat.#: 11836153001): Phenol:Chloroform:Isoamyl Alchohol (Invitrogen, Cat. #:15593031) RNAse (Invitrogen, Cat. #:12091-021) Sequenase Enzyme Kit (Version 2.0, US Biochemical, Cat. #: 70775 dNTP mix (Invitrogen, Cat.#: 18427-013 ) BSA (10 mg/ml , NEB, #B9001S) DTT (0.1 M stock, Invitrogen, #:Y00147) Primer 1 (4 μM): 5'-GACATGTATCCGGATGTNNNNNNNNN-3' ExoSAP-IT (USB, Cat.#:78200) Phusion Polymerase (NEB, Cat#: F-530L, provided with 100 % DMSO, 5X GC buffer) Primer 2 (10 μM stock): 5'-GACATGTATCCGGATGT-3’ BciVI Restriction Enzyme (NEB, Cat. #: R0596L) Agarose (Fisher, Cat #: BP160-500) Protein A-sepharose beads (Sigma, Cat#: P9424) PCR purification, Gel extraction and reaction clean up kits (Qiagen, Cat#s: 28006, 28606, 28206) Glycogen (20μg/μl, Invitrogen, Cat#: 10814-010) Quant-It dsDNA HS assay kit (Invitrogen, Cat#: ) .. 0.2 ml PCR tube strips (Eppendorf, Cat. #: 951010022) 1.5 ml tubes low retention tubes (Eppendorf, Cat. #:022431021) 15 ml tubes (BD Falcon cat. no. 352097) Filtered micropipette tips (Danville Sci., Cat.#s:P1096FR, P1121, P1122, P1126 ) Micropipiettes (1-1000 μl range) Spin-X wash columns (Costar, Cat#:8160) Refrigerated microcentrifuge (Eppendorf model 5415R or equivalent) Minicentrifuge with strip tubes adapter (Fisher Scientific Cat. #:05090-100) Cold room Sonicator (Branson 250 Digitial sonifier) Rotating mixer for 1.5 ml tubes Vortex mixer Thermal Cycler (Eppendorf, master cycle gradient or equivalent) Agarose gel electrophoresis equipment Qubit Flourometer (Invitrogen, Cat #: 32857)

    Plasmid Preparation:

    Article Title: Reconstituting ParA/ParB-mediated transport of DNA cargo
    Article Snippet: .. pBR322:: sopC plasmid (PCR template) PCR Primers for sopC DNA amplification: (Primers synthesized by IDT) 10 mM dNTPs (Life Technologies; Cat. # 18427–013) 100 mM MgSO4 (New England Biolabs, Cat. # M0254S) 2000 U/mL VENT Polymerase (New England Biolabs, Cat. # M0254S) SYBR Gold DNA Stain (Life Technologies, Cat. # S-11494) Illustra MicroSpin S-400HR columns (GE Healthcare, Cat # 27–5140-01) MyOne Streptavidin C1 Dynabeads (Life Technologies, Cat. # 65001) 20 U/μL PstI Restriction Enzyme (New England Biolabs, Cat. # R0140S) Phenol:CHCl3:Isoamyl Alcohol (UltraPure 25:24:1 v/v) Ethanol. .. PCR Thermocycler Tabletop centrifuge Tube rotator BioMag Separator (Advanced Magnetics Inc.) Nanodrop Spectrophotometer.

    Staining:

    Article Title: Reconstituting ParA/ParB-mediated transport of DNA cargo
    Article Snippet: .. pBR322:: sopC plasmid (PCR template) PCR Primers for sopC DNA amplification: (Primers synthesized by IDT) 10 mM dNTPs (Life Technologies; Cat. # 18427–013) 100 mM MgSO4 (New England Biolabs, Cat. # M0254S) 2000 U/mL VENT Polymerase (New England Biolabs, Cat. # M0254S) SYBR Gold DNA Stain (Life Technologies, Cat. # S-11494) Illustra MicroSpin S-400HR columns (GE Healthcare, Cat # 27–5140-01) MyOne Streptavidin C1 Dynabeads (Life Technologies, Cat. # 65001) 20 U/μL PstI Restriction Enzyme (New England Biolabs, Cat. # R0140S) Phenol:CHCl3:Isoamyl Alcohol (UltraPure 25:24:1 v/v) Ethanol. .. PCR Thermocycler Tabletop centrifuge Tube rotator BioMag Separator (Advanced Magnetics Inc.) Nanodrop Spectrophotometer.

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  • Bioz Stars
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  • 90
    Thermo Fisher dntp
    Dntp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 7281 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dntp/product/Thermo Fisher
    Average 90 stars, based on 7281 article reviews
    Price from $9.99 to $1999.99
    dntp - by Bioz Stars, 2020-10
    90/100 stars
      Buy from Supplier

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