dntp mix  (TaKaRa)

 
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    Name:
    dNTP Mixture
    Description:
    Our dNTPs for PCR are 98 pure and are tested for quality control in a variety of applications Individual dNTPs are supplied at a concentration of 100 mM and can be diluted with water or buffer as needed The dNTP Set Cat 4025 provides one of each of the four deoxynucleotide triphosphates
    Catalog Number:
    4030
    Price:
    None
    Size:
    3 2 µmol Each 1 25 mL
    Category:
    dNTPs dNTPs Other PCR related products PCR
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    Structured Review

    TaKaRa dntp mix
    Our dNTPs for PCR are 98 pure and are tested for quality control in a variety of applications Individual dNTPs are supplied at a concentration of 100 mM and can be diluted with water or buffer as needed The dNTP Set Cat 4025 provides one of each of the four deoxynucleotide triphosphates
    https://www.bioz.com/result/dntp mix/product/TaKaRa
    Average 99 stars, based on 492 article reviews
    Price from $9.99 to $1999.99
    dntp mix - by Bioz Stars, 2020-12
    99/100 stars

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    Polymerase Chain Reaction:

    Article Title: Lineage-Specific Distribution of Insertion Sequence Excision Enhancer in Enterotoxigenic Escherichia coli Isolated from Swine
    Article Snippet: .. PCR was performed in a 50-μl reaction mixture containing template DNA, 0.2 μM concentrations of each primer, 0.2 mM concentrations of each deoxynucleoside triphosphate (dNTP), PCR buffer, and 1.25 U of ExTaq DNA polymerase (TaKaRa Bio, Inc., Shiga, Japan) using 30 amplification cycles of 30 s at 95°C, 30 s at 60°C, and 1 min at 72°C. .. PCR amplification of genes encoding various virulence factors (VFs; LT, STa, STb, EAST1, Stx1, Stx2, F4, F5, and F18) was performed as described by Vu-Khac et al. ( ).

    Article Title: Agrobacterium tumefaciens-Mediated Transformation of Valsa mali: An Efficient Tool for Random Insertion Mutagenesis
    Article Snippet: .. PCR reaction was carried out using 50 ng of total DNA, 4 μ M of primer, 0.2 mM of each dNTP, 10 × PCR buffer, and 1.5 units of Taq DNA polymerase (Takara Co. Ltd. Dalian, China). .. The PCR program was as follows: 94°C, 3 min; 94°C, 45 s; 61°C, 45 s; 72°C, 1 min for 35 cycles; 72°C, 5 min.

    Article Title: Generation and Immunity Testing of a Recombinant Adenovirus Expressing NcSRS2-NcGRA7 Fusion Protein of Bovine Neospora caninum
    Article Snippet: .. PCR amplification was performed in a 25 µl reaction system containing 1 µl of each primer (10 µM each), 2 µl of extracted DNA, 2.5 µl of 10× PCR buffer, 2 µl of dNTP (2.5 mM each), and 0.25 µl of TaKaRa (Dalian, China) Taq (5 U/µl). .. The reactions were performed in a thermal cycler (Eppendof, Hamburg, Germany) with the following program: 1 pre-denaturing cycle for 5 min at 94℃; 10 denaturing cycles for 30 sec at 94℃; annealing for 45 sec at 58℃; extension for 60 sec at 72℃; and extension for 7 min at 72℃.

    Article Title: Phylogenetic and Expression Analysis of RNA-binding Proteins with Triple RNA Recognition Motifs in Plants
    Article Snippet: .. Primary RACE PCR was set up with 1 μl of cDNA, 2.5 μl 10× PCR Buffer, 2 μl dNTP mix, 1.5 μl MgCl2 , 1 μl 3′RACE Outer Primer (10 μM 5′-gcgagcacagaattaa tacgact-3′), 1 μl 3′ RACE gene specific primer (10 μM 5′- atattacggaggctactctggtggag-3′ (FL) and 5′-gtggattagatgcatctgtc acggatg-3′, 1 μl Takara Taq, and nuclease free water to a total volume of 25 μl in a microfuge tube. .. PCR was performed using the following parameters: initial denaturation for 5 min at 94℃, followed by 30 cycles of amplification (94℃ 30 s, 63℃ 30 s, 72℃ 30 s), and final extension at 72℃ for 7 min. A secondary PCR for the splice variant was performed using 1 μl of primary PCR reaction.

    Article Title: The first set of universal nuclear protein-coding loci markers for avian phylogenetic and population genetic studies
    Article Snippet: .. The Touchdown PCR was performed in a Veriti96 PCR thermal cycler system (ABI, USA) using a 10 μl reaction containing 2 μl template DNA (10–70 ng totally), with mixed concentrations of 10 × PCR buffer, 20 μM dNTP, 10 mM of each forward and reverse primer, and 5U Taq polymerase (Takara, China). ..

    Article Title: Transmission of Bartonella henselae by Ixodes ricinus
    Article Snippet: .. The PCR cycle was identical for both amplification reactions: an initial denaturation step for 8 min at 94°C; 35 cycles of denaturation for 1 min at 94°C, annealing for 1 min at 54°C, and extension for 1 min at 72°C; and a final extension step at 72°C for 10 min. Each reaction was conducted in a total volume of 25 μL with 0.5 μmol/μL of each primer, 2.5 mmol/L of each dNTP, 2.5 μL of 10× PCR buffer, and 1 U of Taq DNA polymerase (Takara Biomedical Group, Shiga, Japan). ..

    Article Title: Widespread Horizontal Gene Transfer from Double-Stranded RNA Viruses to Eukaryotic Nuclear Genomes ▿Widespread Horizontal Gene Transfer from Double-Stranded RNA Viruses to Eukaryotic Nuclear Genomes ▿ †
    Article Snippet: .. The total volume of each PCR mix was 20 μl and contained 1 μl of DNA template, 2 μl 10× PCR buffer (TaKaRa), 0.4 μl of deoxynucleoside triphosphate (dNTP) mix (10 mM [each dNTP]), 0.2 μl of each primer (20 μM), and 1 unit of Taq DNA polymerase. .. The PCR temperature profile was 94°C for 4 min; 32 cycles of 94°C for 30 s, 55°C for 30 s, and 72°C for 1 min; and final extension at 72°C for 6 min. RT-PCR detection of transcripts of virus-related homologs in the Arabidopsis genome was performed using RevertAid.

    DNA Synthesis:

    Article Title: Chemical Incorporation of Chain-Terminating Nucleoside Analogs as 3′-Blocking DNA Damage and Their Removal by Human ERCC1-XPF Endonuclease
    Article Snippet: .. Resumption of DNA Synthesis by DNA Polymerase after the Removal of CTNAs The hybridized oligonucleotides (400 fmol) were first treated with ERCC1-XPF (230 fmol) in 10 µL of 50 mM Tris-HCl buffer (pH 8.0), containing 2 mM MgCl2 , 0.5 mM DTT and 0.1 mg·mL−1 BSA, at 25 °C for 16 h. To the reaction mixture was added 5 µL of 30 mM Tris-HCl buffer (pH 7.9), containing 150 mM NaCl, 30 mM MgCl2 , 30 mM DTT, 300 µM dNTPs and the Klenow fragment of Escherichia coli DNA polymerase I, lacking the 3′–5′ exonuclease activity (KF− , 0.1 unit, Takara Bio). ..

    Amplification:

    Article Title: Lineage-Specific Distribution of Insertion Sequence Excision Enhancer in Enterotoxigenic Escherichia coli Isolated from Swine
    Article Snippet: .. PCR was performed in a 50-μl reaction mixture containing template DNA, 0.2 μM concentrations of each primer, 0.2 mM concentrations of each deoxynucleoside triphosphate (dNTP), PCR buffer, and 1.25 U of ExTaq DNA polymerase (TaKaRa Bio, Inc., Shiga, Japan) using 30 amplification cycles of 30 s at 95°C, 30 s at 60°C, and 1 min at 72°C. .. PCR amplification of genes encoding various virulence factors (VFs; LT, STa, STb, EAST1, Stx1, Stx2, F4, F5, and F18) was performed as described by Vu-Khac et al. ( ).

    Article Title: Generation and Immunity Testing of a Recombinant Adenovirus Expressing NcSRS2-NcGRA7 Fusion Protein of Bovine Neospora caninum
    Article Snippet: .. PCR amplification was performed in a 25 µl reaction system containing 1 µl of each primer (10 µM each), 2 µl of extracted DNA, 2.5 µl of 10× PCR buffer, 2 µl of dNTP (2.5 mM each), and 0.25 µl of TaKaRa (Dalian, China) Taq (5 U/µl). .. The reactions were performed in a thermal cycler (Eppendof, Hamburg, Germany) with the following program: 1 pre-denaturing cycle for 5 min at 94℃; 10 denaturing cycles for 30 sec at 94℃; annealing for 45 sec at 58℃; extension for 60 sec at 72℃; and extension for 7 min at 72℃.

    Article Title: Transmission of Bartonella henselae by Ixodes ricinus
    Article Snippet: .. The PCR cycle was identical for both amplification reactions: an initial denaturation step for 8 min at 94°C; 35 cycles of denaturation for 1 min at 94°C, annealing for 1 min at 54°C, and extension for 1 min at 72°C; and a final extension step at 72°C for 10 min. Each reaction was conducted in a total volume of 25 μL with 0.5 μmol/μL of each primer, 2.5 mmol/L of each dNTP, 2.5 μL of 10× PCR buffer, and 1 U of Taq DNA polymerase (Takara Biomedical Group, Shiga, Japan). ..

    Activity Assay:

    Article Title: Chemical Incorporation of Chain-Terminating Nucleoside Analogs as 3′-Blocking DNA Damage and Their Removal by Human ERCC1-XPF Endonuclease
    Article Snippet: .. Resumption of DNA Synthesis by DNA Polymerase after the Removal of CTNAs The hybridized oligonucleotides (400 fmol) were first treated with ERCC1-XPF (230 fmol) in 10 µL of 50 mM Tris-HCl buffer (pH 8.0), containing 2 mM MgCl2 , 0.5 mM DTT and 0.1 mg·mL−1 BSA, at 25 °C for 16 h. To the reaction mixture was added 5 µL of 30 mM Tris-HCl buffer (pH 7.9), containing 150 mM NaCl, 30 mM MgCl2 , 30 mM DTT, 300 µM dNTPs and the Klenow fragment of Escherichia coli DNA polymerase I, lacking the 3′–5′ exonuclease activity (KF− , 0.1 unit, Takara Bio). ..

    Touchdown PCR:

    Article Title: The first set of universal nuclear protein-coding loci markers for avian phylogenetic and population genetic studies
    Article Snippet: .. The Touchdown PCR was performed in a Veriti96 PCR thermal cycler system (ABI, USA) using a 10 μl reaction containing 2 μl template DNA (10–70 ng totally), with mixed concentrations of 10 × PCR buffer, 20 μM dNTP, 10 mM of each forward and reverse primer, and 5U Taq polymerase (Takara, China). ..

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  • 92
    TaKaRa telomeric repeat amplification protocol trap lig assay dntp mix
    The Pt-tripod binds the intramolecular hybrid-1 human <t>telomeric</t> G-quadruplex and inhibits telomerase activity. a The chemical structure of the Pt-tripod with proton numbering. b The folding topology of the hybrid-1 human telomeric G-quadruplex adopted by Tel26 in K + solution 44 . Deep gray box = ( syn ) guanine, light gray box = ( anti ) guanine. c Imino proton regions of the 1D 1 H NMR titration spectra of Tel26 interacting with Pt-tripod in 100 mM K + , pH 7.0 solution, at 25 °C. Ratios of Pt-tripod/Tel26 are shown in the spectra. The imino assignments are labeled. Peaks arising from the new complexes are marked with asterisks. d CD spectra of the Tel26 titrated by the Pt-tripod in 100 mM K + solution. Ratios of Pt-tripod/Tel26 are shown in the spectra. e The Pt-tripod showed excellent telomerase inhibition property by the <t>TRAP-LIG</t> assay. The band labeled as ITAS is an internal control primer. The IC 50 value is determined to be 1.22 ± 0.10 μM. Error value represents the standard deviation, n = 3
    Telomeric Repeat Amplification Protocol Trap Lig Assay Dntp Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/telomeric repeat amplification protocol trap lig assay dntp mix/product/TaKaRa
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    telomeric repeat amplification protocol trap lig assay dntp mix - by Bioz Stars, 2020-12
    92/100 stars
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    99
    TaKaRa dntp mix
    The Pt-tripod binds the intramolecular hybrid-1 human <t>telomeric</t> G-quadruplex and inhibits telomerase activity. a The chemical structure of the Pt-tripod with proton numbering. b The folding topology of the hybrid-1 human telomeric G-quadruplex adopted by Tel26 in K + solution 44 . Deep gray box = ( syn ) guanine, light gray box = ( anti ) guanine. c Imino proton regions of the 1D 1 H NMR titration spectra of Tel26 interacting with Pt-tripod in 100 mM K + , pH 7.0 solution, at 25 °C. Ratios of Pt-tripod/Tel26 are shown in the spectra. The imino assignments are labeled. Peaks arising from the new complexes are marked with asterisks. d CD spectra of the Tel26 titrated by the Pt-tripod in 100 mM K + solution. Ratios of Pt-tripod/Tel26 are shown in the spectra. e The Pt-tripod showed excellent telomerase inhibition property by the <t>TRAP-LIG</t> assay. The band labeled as ITAS is an internal control primer. The IC 50 value is determined to be 1.22 ± 0.10 μM. Error value represents the standard deviation, n = 3
    Dntp Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 660 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dntp mix/product/TaKaRa
    Average 99 stars, based on 660 article reviews
    Price from $9.99 to $1999.99
    dntp mix - by Bioz Stars, 2020-12
    99/100 stars
      Buy from Supplier

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    The Pt-tripod binds the intramolecular hybrid-1 human telomeric G-quadruplex and inhibits telomerase activity. a The chemical structure of the Pt-tripod with proton numbering. b The folding topology of the hybrid-1 human telomeric G-quadruplex adopted by Tel26 in K + solution 44 . Deep gray box = ( syn ) guanine, light gray box = ( anti ) guanine. c Imino proton regions of the 1D 1 H NMR titration spectra of Tel26 interacting with Pt-tripod in 100 mM K + , pH 7.0 solution, at 25 °C. Ratios of Pt-tripod/Tel26 are shown in the spectra. The imino assignments are labeled. Peaks arising from the new complexes are marked with asterisks. d CD spectra of the Tel26 titrated by the Pt-tripod in 100 mM K + solution. Ratios of Pt-tripod/Tel26 are shown in the spectra. e The Pt-tripod showed excellent telomerase inhibition property by the TRAP-LIG assay. The band labeled as ITAS is an internal control primer. The IC 50 value is determined to be 1.22 ± 0.10 μM. Error value represents the standard deviation, n = 3

    Journal: Nature Communications

    Article Title: Solution structures of multiple G-quadruplex complexes induced by a platinum(II)-based tripod reveal dynamic binding

    doi: 10.1038/s41467-018-05810-4

    Figure Lengend Snippet: The Pt-tripod binds the intramolecular hybrid-1 human telomeric G-quadruplex and inhibits telomerase activity. a The chemical structure of the Pt-tripod with proton numbering. b The folding topology of the hybrid-1 human telomeric G-quadruplex adopted by Tel26 in K + solution 44 . Deep gray box = ( syn ) guanine, light gray box = ( anti ) guanine. c Imino proton regions of the 1D 1 H NMR titration spectra of Tel26 interacting with Pt-tripod in 100 mM K + , pH 7.0 solution, at 25 °C. Ratios of Pt-tripod/Tel26 are shown in the spectra. The imino assignments are labeled. Peaks arising from the new complexes are marked with asterisks. d CD spectra of the Tel26 titrated by the Pt-tripod in 100 mM K + solution. Ratios of Pt-tripod/Tel26 are shown in the spectra. e The Pt-tripod showed excellent telomerase inhibition property by the TRAP-LIG assay. The band labeled as ITAS is an internal control primer. The IC 50 value is determined to be 1.22 ± 0.10 μM. Error value represents the standard deviation, n = 3

    Article Snippet: Telomeric repeat amplification protocol (TRAP-LIG) assay dNTP mix, RNase inhibitor, and Taq polymerase were purchased from TaKaRa Biotechnology.

    Techniques: Activity Assay, Nuclear Magnetic Resonance, Titration, Labeling, Inhibition, Standard Deviation