dntp mix  (TaKaRa)

 
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    Name:
    Advantage 2 Polymerase Mix
    Description:
    Advantage 2 Polymerase Mix is an optimized blend of PCR enzymes that is a proven balance of high yield and high fidelity with 3X higher fidelity than regular Taq for cDNA amplification and library construction With an automatic hot start feature our Advantage 2 Polymerase Mix can readily amplify a wide range of DNA templates including long templates up to 18 kb and complex genomic DNA up to 6kb The Advantage 2 Polymerase Mix includes two optimized buffers dNTPs need to be purchased separately
    Catalog Number:
    639202
    Price:
    None
    Size:
    5 x 100 Rxns
    Category:
    Advantage 2 Polymerase Mix Advantage 2 products High yield PCR PCR
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    Structured Review

    TaKaRa dntp mix
    Advantage 2 Polymerase Mix is an optimized blend of PCR enzymes that is a proven balance of high yield and high fidelity with 3X higher fidelity than regular Taq for cDNA amplification and library construction With an automatic hot start feature our Advantage 2 Polymerase Mix can readily amplify a wide range of DNA templates including long templates up to 18 kb and complex genomic DNA up to 6kb The Advantage 2 Polymerase Mix includes two optimized buffers dNTPs need to be purchased separately
    https://www.bioz.com/result/dntp mix/product/TaKaRa
    Average 99 stars, based on 492 article reviews
    Price from $9.99 to $1999.99
    dntp mix - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Molecular Cloning:

    Article Title: Molecular cloning and tissue distribution of mammalian L-threonine 3-dehydrogenases
    Article Snippet: .. Molecular cloning of murine and porcine L-threonine dehydrogenase cDNAs Clones encoding the mouse L-threonine dehydrogenase cDNA sequence were obtained by touchdown PCR amplification from mouse liver and lung cDNAs using the Advantage cDNA polymerase mix (Clontech, UK) on a Perkin-Elmer 2400 thermocycler. .. The cycle conditions for the first 10 cycles were 94°C for 5 sec, 72°C less 0.4°C per cycle for 3 min and for the next 20 cycles 94°C for 5 sec, 68°C for 10 sec, 72°C for 3 min per cycle using primers (100 nM) derived from the sequence of the mouse ESTs D21787, forward 5'-CCGGCTCCCGCGTGGCGTTCTCAGCATCCA-3' and AV100443, first reverse 5' TTTTTTTTTTTTTTTTTGATACTTAAATTG-3' and second reverse 5'-TTTTTTTTTTTGCAAGCGATCGTT-3' (Amersham-Pharmacia Biotech, UK).

    Amplification:

    Article Title: E47 is required for V(D)J recombinase activity in common lymphoid progenitors
    Article Snippet: .. In the first round, PCR amplification was performed in a total volume of 50 μl containing 0.2 mM dNTP, 1 μl CLONTECH Advantage cDNA Polymerase Mix, 1X CLONTECH Advantage PCR Buffer, 0.5 mg/ml BSA, and 0.4 μM of each primer. ..

    Article Title: Molecular cloning and tissue distribution of mammalian L-threonine 3-dehydrogenases
    Article Snippet: .. Molecular cloning of murine and porcine L-threonine dehydrogenase cDNAs Clones encoding the mouse L-threonine dehydrogenase cDNA sequence were obtained by touchdown PCR amplification from mouse liver and lung cDNAs using the Advantage cDNA polymerase mix (Clontech, UK) on a Perkin-Elmer 2400 thermocycler. .. The cycle conditions for the first 10 cycles were 94°C for 5 sec, 72°C less 0.4°C per cycle for 3 min and for the next 20 cycles 94°C for 5 sec, 68°C for 10 sec, 72°C for 3 min per cycle using primers (100 nM) derived from the sequence of the mouse ESTs D21787, forward 5'-CCGGCTCCCGCGTGGCGTTCTCAGCATCCA-3' and AV100443, first reverse 5' TTTTTTTTTTTTTTTTTGATACTTAAATTG-3' and second reverse 5'-TTTTTTTTTTTGCAAGCGATCGTT-3' (Amersham-Pharmacia Biotech, UK).

    Article Title: Molecular Characterization of 3-Phosphoglycerate Dehydrogenase Deficiency--a Neurometabolic Disorder Associated with Reduced L-Serine Biosynthesis
    Article Snippet: .. The complete coding sequence of PHGDH was amplified using primers f2–r10 and Advantage cDNA polymerase mix (Clontech) for 33 cycles. .. An appropriately sized PCR product was cloned in pCR2.1 (Invitrogen).

    Article Title: Identification of differentially expressed genes in isogenic highly metastatic and poorly metastatic cell lines of R3230AC rat mammary adenocarcinoma
    Article Snippet: .. New hybrid molecules with different adaptors on each end were amplified with 50 × Advantage cDNA Polymerase mix (Clontech) in a Perkin Elmer GeneAmp PCR System 2000 thermocycler (27 cycles: 94 °C, 10 s; 66 °C, 30 s; 72 °C, 1.5 min). ..

    Article Title: An Ebox Element in the Proximal Gata4 Promoter Is Required for Gata4 Expression In Vivo
    Article Snippet: .. Sequences for the 5′ (3.2 kb) and 3′ (3.0 kb) homologous arms were obtained by PCR amplification of FVB/n mouse genomic DNA using the Advantage II DNA polymerase mix (Clontech). .. These PCR products were cloned into the pGEM-T plasmid (Promega, Madison, WI) and sequences were confirmed by sequencing.

    Article Title: Identification and Characterization of Human Orthologues to Saccharomyces cerevisiae Upf2 Protein and Upf3 Protein (Caenorhabditis elegans SMG-4)
    Article Snippet: .. To generate pCI-Neo-hUPF3-X WT, the entire coding region of hUPF3-X cDNA together with 19 nt of 5′ untranslated region (UTR) and the two consecutive termination codons of the 3′ UTR were PCR amplified as two fragments that harbored an overlapping Eco RI site by using the Advantage cDNA polymerase mix (Clontech) and HeLa-cell Marathon-Ready cDNA (Clontech). ..

    Clone Assay:

    Article Title: Molecular cloning and tissue distribution of mammalian L-threonine 3-dehydrogenases
    Article Snippet: .. Molecular cloning of murine and porcine L-threonine dehydrogenase cDNAs Clones encoding the mouse L-threonine dehydrogenase cDNA sequence were obtained by touchdown PCR amplification from mouse liver and lung cDNAs using the Advantage cDNA polymerase mix (Clontech, UK) on a Perkin-Elmer 2400 thermocycler. .. The cycle conditions for the first 10 cycles were 94°C for 5 sec, 72°C less 0.4°C per cycle for 3 min and for the next 20 cycles 94°C for 5 sec, 68°C for 10 sec, 72°C for 3 min per cycle using primers (100 nM) derived from the sequence of the mouse ESTs D21787, forward 5'-CCGGCTCCCGCGTGGCGTTCTCAGCATCCA-3' and AV100443, first reverse 5' TTTTTTTTTTTTTTTTTGATACTTAAATTG-3' and second reverse 5'-TTTTTTTTTTTGCAAGCGATCGTT-3' (Amersham-Pharmacia Biotech, UK).

    Hot Start PCR:

    Article Title: Loss of Heterozygosity Analysis Using Whole Genome Amplification, Cell Sorting, and Fluorescence-Based PCR
    Article Snippet: .. To increase throughput and enhance primer-binding specificity, we used Advantage PCR enzyme mix (Clontech), a mix of Tth DNA polymerase, a second proofreading polymerase, and TaqStart Antibody, which provides built-in hot-start PCR. .. Locus-specific reactions were performed in a total volume of 8 μl with 0.08 μl of this enzyme mix, 1× buffer with 3 m m magnesium acetate in the concentration supplied by the manufacturer (Clontech), and 200 μ m dNTPs.

    cDNA Library Assay:

    Article Title: Identification and Characterization of Human Orthologues to Saccharomyces cerevisiae Upf2 Protein and Upf3 Protein (Caenorhabditis elegans SMG-4)
    Article Snippet: .. Primers were generated from these sequences and used to amplify a HeLa-cell Marathon cDNA library (Clontech) by using the Advantage cDNA polymerase mix (Clontech) and RACE-PCR. .. In order to ensure that the resulting cDNAs harbored an unmutagenized coding region, subsequent studies were confined to those cDNAs that harbored a coding region identical to (i) at least two out of three independently amplified coding regions and (ii) available database sequences.

    Generated:

    Article Title: Identification and Characterization of Human Orthologues to Saccharomyces cerevisiae Upf2 Protein and Upf3 Protein (Caenorhabditis elegans SMG-4)
    Article Snippet: .. Primers were generated from these sequences and used to amplify a HeLa-cell Marathon cDNA library (Clontech) by using the Advantage cDNA polymerase mix (Clontech) and RACE-PCR. .. In order to ensure that the resulting cDNAs harbored an unmutagenized coding region, subsequent studies were confined to those cDNAs that harbored a coding region identical to (i) at least two out of three independently amplified coding regions and (ii) available database sequences.

    Polymerase Chain Reaction:

    Article Title: E47 is required for V(D)J recombinase activity in common lymphoid progenitors
    Article Snippet: .. In the first round, PCR amplification was performed in a total volume of 50 μl containing 0.2 mM dNTP, 1 μl CLONTECH Advantage cDNA Polymerase Mix, 1X CLONTECH Advantage PCR Buffer, 0.5 mg/ml BSA, and 0.4 μM of each primer. ..

    Article Title: Identification of differentially expressed genes in isogenic highly metastatic and poorly metastatic cell lines of R3230AC rat mammary adenocarcinoma
    Article Snippet: .. New hybrid molecules with different adaptors on each end were amplified with 50 × Advantage cDNA Polymerase mix (Clontech) in a Perkin Elmer GeneAmp PCR System 2000 thermocycler (27 cycles: 94 °C, 10 s; 66 °C, 30 s; 72 °C, 1.5 min). ..

    Article Title: An Ebox Element in the Proximal Gata4 Promoter Is Required for Gata4 Expression In Vivo
    Article Snippet: .. Sequences for the 5′ (3.2 kb) and 3′ (3.0 kb) homologous arms were obtained by PCR amplification of FVB/n mouse genomic DNA using the Advantage II DNA polymerase mix (Clontech). .. These PCR products were cloned into the pGEM-T plasmid (Promega, Madison, WI) and sequences were confirmed by sequencing.

    Article Title: Identification and Characterization of Human Orthologues to Saccharomyces cerevisiae Upf2 Protein and Upf3 Protein (Caenorhabditis elegans SMG-4)
    Article Snippet: .. To generate pCI-Neo-hUPF3-X WT, the entire coding region of hUPF3-X cDNA together with 19 nt of 5′ untranslated region (UTR) and the two consecutive termination codons of the 3′ UTR were PCR amplified as two fragments that harbored an overlapping Eco RI site by using the Advantage cDNA polymerase mix (Clontech) and HeLa-cell Marathon-Ready cDNA (Clontech). ..

    Article Title: Loss of Heterozygosity Analysis Using Whole Genome Amplification, Cell Sorting, and Fluorescence-Based PCR
    Article Snippet: .. To increase throughput and enhance primer-binding specificity, we used Advantage PCR enzyme mix (Clontech), a mix of Tth DNA polymerase, a second proofreading polymerase, and TaqStart Antibody, which provides built-in hot-start PCR. .. Locus-specific reactions were performed in a total volume of 8 μl with 0.08 μl of this enzyme mix, 1× buffer with 3 m m magnesium acetate in the concentration supplied by the manufacturer (Clontech), and 200 μ m dNTPs.

    Touchdown PCR:

    Article Title: Molecular cloning and tissue distribution of mammalian L-threonine 3-dehydrogenases
    Article Snippet: .. Molecular cloning of murine and porcine L-threonine dehydrogenase cDNAs Clones encoding the mouse L-threonine dehydrogenase cDNA sequence were obtained by touchdown PCR amplification from mouse liver and lung cDNAs using the Advantage cDNA polymerase mix (Clontech, UK) on a Perkin-Elmer 2400 thermocycler. .. The cycle conditions for the first 10 cycles were 94°C for 5 sec, 72°C less 0.4°C per cycle for 3 min and for the next 20 cycles 94°C for 5 sec, 68°C for 10 sec, 72°C for 3 min per cycle using primers (100 nM) derived from the sequence of the mouse ESTs D21787, forward 5'-CCGGCTCCCGCGTGGCGTTCTCAGCATCCA-3' and AV100443, first reverse 5' TTTTTTTTTTTTTTTTTGATACTTAAATTG-3' and second reverse 5'-TTTTTTTTTTTGCAAGCGATCGTT-3' (Amersham-Pharmacia Biotech, UK).

    Sequencing:

    Article Title: Molecular cloning and tissue distribution of mammalian L-threonine 3-dehydrogenases
    Article Snippet: .. Molecular cloning of murine and porcine L-threonine dehydrogenase cDNAs Clones encoding the mouse L-threonine dehydrogenase cDNA sequence were obtained by touchdown PCR amplification from mouse liver and lung cDNAs using the Advantage cDNA polymerase mix (Clontech, UK) on a Perkin-Elmer 2400 thermocycler. .. The cycle conditions for the first 10 cycles were 94°C for 5 sec, 72°C less 0.4°C per cycle for 3 min and for the next 20 cycles 94°C for 5 sec, 68°C for 10 sec, 72°C for 3 min per cycle using primers (100 nM) derived from the sequence of the mouse ESTs D21787, forward 5'-CCGGCTCCCGCGTGGCGTTCTCAGCATCCA-3' and AV100443, first reverse 5' TTTTTTTTTTTTTTTTTGATACTTAAATTG-3' and second reverse 5'-TTTTTTTTTTTGCAAGCGATCGTT-3' (Amersham-Pharmacia Biotech, UK).

    Article Title: Molecular Characterization of 3-Phosphoglycerate Dehydrogenase Deficiency--a Neurometabolic Disorder Associated with Reduced L-Serine Biosynthesis
    Article Snippet: .. The complete coding sequence of PHGDH was amplified using primers f2–r10 and Advantage cDNA polymerase mix (Clontech) for 33 cycles. .. An appropriately sized PCR product was cloned in pCR2.1 (Invitrogen).

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  • 92
    TaKaRa telomeric repeat amplification protocol trap lig assay dntp mix
    The Pt-tripod binds the intramolecular hybrid-1 human <t>telomeric</t> G-quadruplex and inhibits telomerase activity. a The chemical structure of the Pt-tripod with proton numbering. b The folding topology of the hybrid-1 human telomeric G-quadruplex adopted by Tel26 in K + solution 44 . Deep gray box = ( syn ) guanine, light gray box = ( anti ) guanine. c Imino proton regions of the 1D 1 H NMR titration spectra of Tel26 interacting with Pt-tripod in 100 mM K + , pH 7.0 solution, at 25 °C. Ratios of Pt-tripod/Tel26 are shown in the spectra. The imino assignments are labeled. Peaks arising from the new complexes are marked with asterisks. d CD spectra of the Tel26 titrated by the Pt-tripod in 100 mM K + solution. Ratios of Pt-tripod/Tel26 are shown in the spectra. e The Pt-tripod showed excellent telomerase inhibition property by the <t>TRAP-LIG</t> assay. The band labeled as ITAS is an internal control primer. The IC 50 value is determined to be 1.22 ± 0.10 μM. Error value represents the standard deviation, n = 3
    Telomeric Repeat Amplification Protocol Trap Lig Assay Dntp Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/telomeric repeat amplification protocol trap lig assay dntp mix/product/TaKaRa
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    telomeric repeat amplification protocol trap lig assay dntp mix - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    99
    TaKaRa dntp mix
    The Pt-tripod binds the intramolecular hybrid-1 human <t>telomeric</t> G-quadruplex and inhibits telomerase activity. a The chemical structure of the Pt-tripod with proton numbering. b The folding topology of the hybrid-1 human telomeric G-quadruplex adopted by Tel26 in K + solution 44 . Deep gray box = ( syn ) guanine, light gray box = ( anti ) guanine. c Imino proton regions of the 1D 1 H NMR titration spectra of Tel26 interacting with Pt-tripod in 100 mM K + , pH 7.0 solution, at 25 °C. Ratios of Pt-tripod/Tel26 are shown in the spectra. The imino assignments are labeled. Peaks arising from the new complexes are marked with asterisks. d CD spectra of the Tel26 titrated by the Pt-tripod in 100 mM K + solution. Ratios of Pt-tripod/Tel26 are shown in the spectra. e The Pt-tripod showed excellent telomerase inhibition property by the <t>TRAP-LIG</t> assay. The band labeled as ITAS is an internal control primer. The IC 50 value is determined to be 1.22 ± 0.10 μM. Error value represents the standard deviation, n = 3
    Dntp Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 492 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dntp mix/product/TaKaRa
    Average 99 stars, based on 492 article reviews
    Price from $9.99 to $1999.99
    dntp mix - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    The Pt-tripod binds the intramolecular hybrid-1 human telomeric G-quadruplex and inhibits telomerase activity. a The chemical structure of the Pt-tripod with proton numbering. b The folding topology of the hybrid-1 human telomeric G-quadruplex adopted by Tel26 in K + solution 44 . Deep gray box = ( syn ) guanine, light gray box = ( anti ) guanine. c Imino proton regions of the 1D 1 H NMR titration spectra of Tel26 interacting with Pt-tripod in 100 mM K + , pH 7.0 solution, at 25 °C. Ratios of Pt-tripod/Tel26 are shown in the spectra. The imino assignments are labeled. Peaks arising from the new complexes are marked with asterisks. d CD spectra of the Tel26 titrated by the Pt-tripod in 100 mM K + solution. Ratios of Pt-tripod/Tel26 are shown in the spectra. e The Pt-tripod showed excellent telomerase inhibition property by the TRAP-LIG assay. The band labeled as ITAS is an internal control primer. The IC 50 value is determined to be 1.22 ± 0.10 μM. Error value represents the standard deviation, n = 3

    Journal: Nature Communications

    Article Title: Solution structures of multiple G-quadruplex complexes induced by a platinum(II)-based tripod reveal dynamic binding

    doi: 10.1038/s41467-018-05810-4

    Figure Lengend Snippet: The Pt-tripod binds the intramolecular hybrid-1 human telomeric G-quadruplex and inhibits telomerase activity. a The chemical structure of the Pt-tripod with proton numbering. b The folding topology of the hybrid-1 human telomeric G-quadruplex adopted by Tel26 in K + solution 44 . Deep gray box = ( syn ) guanine, light gray box = ( anti ) guanine. c Imino proton regions of the 1D 1 H NMR titration spectra of Tel26 interacting with Pt-tripod in 100 mM K + , pH 7.0 solution, at 25 °C. Ratios of Pt-tripod/Tel26 are shown in the spectra. The imino assignments are labeled. Peaks arising from the new complexes are marked with asterisks. d CD spectra of the Tel26 titrated by the Pt-tripod in 100 mM K + solution. Ratios of Pt-tripod/Tel26 are shown in the spectra. e The Pt-tripod showed excellent telomerase inhibition property by the TRAP-LIG assay. The band labeled as ITAS is an internal control primer. The IC 50 value is determined to be 1.22 ± 0.10 μM. Error value represents the standard deviation, n = 3

    Article Snippet: Telomeric repeat amplification protocol (TRAP-LIG) assay dNTP mix, RNase inhibitor, and Taq polymerase were purchased from TaKaRa Biotechnology.

    Techniques: Activity Assay, Nuclear Magnetic Resonance, Titration, Labeling, Inhibition, Standard Deviation