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anti-goat dnp63  (R&D Systems Hematology)
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R&D Systems Hematology anti-goat dnp63
(a) Recombination PCR for RPM basal organoids for indicated alleles pre- and post-treatment with TAT-Cre recombinase or Ad-CMV-Cre at two concentrations (2.5e7 or 5e7 pfu). *Organoids subject to spinoculation with CMV-Cre virus. Red font indicates condition used for subsequent allografting. (b) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA) for DAPI (nuclei, blue), KI67 (proliferation, orange), and ASCL1 (NE cell, green). Positive controls for KI67 and ASCL1 (bottom panel) is an RPR2 SCLC lung tumour. Quantification via CellProfiler of KI67 positivity per organoid from indicated conditions (right). Number of organoids quantified per group is labeled. One-way ANOVA with Tukey’s correction. *** p<0.003, ** p<0.005, ns=not significant, p>0.05. Error bars represent mean ± SD. (c) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA): Left) DAPI (nuclei, blue), NEUROD1 (neuronal cell, green), <t>DNP63</t> (basal cell, yellow) and KRT8 (luminal basal cell, red). Positive control (+) for NEUROD1 is an RPM olfactory neuroblastoma tumour and for basal markers is an SNL GEMM lung tumour . Right) DAPI (nuclei, blue), FOXJ1 (ciliated cell, purple), CCSP (SCGB1A1, club cell, green) and KRT8 (luminal basal cell, red). Positive control (+) control for FOXJ1 and CCSP is airway from a normal mouse lung, and for KRT8 is an SNL GEMM lung tumour. (d) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA) for DAPI (nuclei, blue) and POU2F3 (tuft cell, green). Positive control (+) is an RPMA olfactory neuroblastoma tumour . All co-IF scale bars=150 μm.
Anti Goat Dnp63, supplied by R&D Systems Hematology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dnp63  (R&D Systems Hematology)
90
R&D Systems Hematology dnp63
(a) Recombination PCR for RPM basal organoids for indicated alleles pre- and post-treatment with TAT-Cre recombinase or Ad-CMV-Cre at two concentrations (2.5e7 or 5e7 pfu). *Organoids subject to spinoculation with CMV-Cre virus. Red font indicates condition used for subsequent allografting. (b) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA) for DAPI (nuclei, blue), KI67 (proliferation, orange), and ASCL1 (NE cell, green). Positive controls for KI67 and ASCL1 (bottom panel) is an RPR2 SCLC lung tumour. Quantification via CellProfiler of KI67 positivity per organoid from indicated conditions (right). Number of organoids quantified per group is labeled. One-way ANOVA with Tukey’s correction. *** p<0.003, ** p<0.005, ns=not significant, p>0.05. Error bars represent mean ± SD. (c) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA): Left) DAPI (nuclei, blue), NEUROD1 (neuronal cell, green), <t>DNP63</t> (basal cell, yellow) and KRT8 (luminal basal cell, red). Positive control (+) for NEUROD1 is an RPM olfactory neuroblastoma tumour and for basal markers is an SNL GEMM lung tumour . Right) DAPI (nuclei, blue), FOXJ1 (ciliated cell, purple), CCSP (SCGB1A1, club cell, green) and KRT8 (luminal basal cell, red). Positive control (+) control for FOXJ1 and CCSP is airway from a normal mouse lung, and for KRT8 is an SNL GEMM lung tumour. (d) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA) for DAPI (nuclei, blue) and POU2F3 (tuft cell, green). Positive control (+) is an RPMA olfactory neuroblastoma tumour . All co-IF scale bars=150 μm.
Dnp63, supplied by R&D Systems Hematology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dnp63  (Addgene inc)
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Addgene inc dnp63
(a) Recombination PCR for RPM basal organoids for indicated alleles pre- and post-treatment with TAT-Cre recombinase or Ad-CMV-Cre at two concentrations (2.5e7 or 5e7 pfu). *Organoids subject to spinoculation with CMV-Cre virus. Red font indicates condition used for subsequent allografting. (b) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA) for DAPI (nuclei, blue), KI67 (proliferation, orange), and ASCL1 (NE cell, green). Positive controls for KI67 and ASCL1 (bottom panel) is an RPR2 SCLC lung tumour. Quantification via CellProfiler of KI67 positivity per organoid from indicated conditions (right). Number of organoids quantified per group is labeled. One-way ANOVA with Tukey’s correction. *** p<0.003, ** p<0.005, ns=not significant, p>0.05. Error bars represent mean ± SD. (c) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA): Left) DAPI (nuclei, blue), NEUROD1 (neuronal cell, green), <t>DNP63</t> (basal cell, yellow) and KRT8 (luminal basal cell, red). Positive control (+) for NEUROD1 is an RPM olfactory neuroblastoma tumour and for basal markers is an SNL GEMM lung tumour . Right) DAPI (nuclei, blue), FOXJ1 (ciliated cell, purple), CCSP (SCGB1A1, club cell, green) and KRT8 (luminal basal cell, red). Positive control (+) control for FOXJ1 and CCSP is airway from a normal mouse lung, and for KRT8 is an SNL GEMM lung tumour. (d) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA) for DAPI (nuclei, blue) and POU2F3 (tuft cell, green). Positive control (+) is an RPMA olfactory neuroblastoma tumour . All co-IF scale bars=150 μm.
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dnp63-specific antibody clone bc28  (Biocare Medical)
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Biocare Medical dnp63-specific antibody clone bc28
(a) Recombination PCR for RPM basal organoids for indicated alleles pre- and post-treatment with TAT-Cre recombinase or Ad-CMV-Cre at two concentrations (2.5e7 or 5e7 pfu). *Organoids subject to spinoculation with CMV-Cre virus. Red font indicates condition used for subsequent allografting. (b) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA) for DAPI (nuclei, blue), KI67 (proliferation, orange), and ASCL1 (NE cell, green). Positive controls for KI67 and ASCL1 (bottom panel) is an RPR2 SCLC lung tumour. Quantification via CellProfiler of KI67 positivity per organoid from indicated conditions (right). Number of organoids quantified per group is labeled. One-way ANOVA with Tukey’s correction. *** p<0.003, ** p<0.005, ns=not significant, p>0.05. Error bars represent mean ± SD. (c) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA): Left) DAPI (nuclei, blue), NEUROD1 (neuronal cell, green), <t>DNP63</t> (basal cell, yellow) and KRT8 (luminal basal cell, red). Positive control (+) for NEUROD1 is an RPM olfactory neuroblastoma tumour and for basal markers is an SNL GEMM lung tumour . Right) DAPI (nuclei, blue), FOXJ1 (ciliated cell, purple), CCSP (SCGB1A1, club cell, green) and KRT8 (luminal basal cell, red). Positive control (+) control for FOXJ1 and CCSP is airway from a normal mouse lung, and for KRT8 is an SNL GEMM lung tumour. (d) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA) for DAPI (nuclei, blue) and POU2F3 (tuft cell, green). Positive control (+) is an RPMA olfactory neuroblastoma tumour . All co-IF scale bars=150 μm.
Dnp63 Specific Antibody Clone Bc28, supplied by Biocare Medical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibody target dnp63  (STEMCELL Technologies Inc)
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STEMCELL Technologies Inc antibody target dnp63
(a) Recombination PCR for RPM basal organoids for indicated alleles pre- and post-treatment with TAT-Cre recombinase or Ad-CMV-Cre at two concentrations (2.5e7 or 5e7 pfu). *Organoids subject to spinoculation with CMV-Cre virus. Red font indicates condition used for subsequent allografting. (b) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA) for DAPI (nuclei, blue), KI67 (proliferation, orange), and ASCL1 (NE cell, green). Positive controls for KI67 and ASCL1 (bottom panel) is an RPR2 SCLC lung tumour. Quantification via CellProfiler of KI67 positivity per organoid from indicated conditions (right). Number of organoids quantified per group is labeled. One-way ANOVA with Tukey’s correction. *** p<0.003, ** p<0.005, ns=not significant, p>0.05. Error bars represent mean ± SD. (c) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA): Left) DAPI (nuclei, blue), NEUROD1 (neuronal cell, green), <t>DNP63</t> (basal cell, yellow) and KRT8 (luminal basal cell, red). Positive control (+) for NEUROD1 is an RPM olfactory neuroblastoma tumour and for basal markers is an SNL GEMM lung tumour . Right) DAPI (nuclei, blue), FOXJ1 (ciliated cell, purple), CCSP (SCGB1A1, club cell, green) and KRT8 (luminal basal cell, red). Positive control (+) control for FOXJ1 and CCSP is airway from a normal mouse lung, and for KRT8 is an SNL GEMM lung tumour. (d) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA) for DAPI (nuclei, blue) and POU2F3 (tuft cell, green). Positive control (+) is an RPMA olfactory neuroblastoma tumour . All co-IF scale bars=150 μm.
Antibody Target Dnp63, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti dnp63  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc anti dnp63
(a) Recombination PCR for RPM basal organoids for indicated alleles pre- and post-treatment with TAT-Cre recombinase or Ad-CMV-Cre at two concentrations (2.5e7 or 5e7 pfu). *Organoids subject to spinoculation with CMV-Cre virus. Red font indicates condition used for subsequent allografting. (b) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA) for DAPI (nuclei, blue), KI67 (proliferation, orange), and ASCL1 (NE cell, green). Positive controls for KI67 and ASCL1 (bottom panel) is an RPR2 SCLC lung tumour. Quantification via CellProfiler of KI67 positivity per organoid from indicated conditions (right). Number of organoids quantified per group is labeled. One-way ANOVA with Tukey’s correction. *** p<0.003, ** p<0.005, ns=not significant, p>0.05. Error bars represent mean ± SD. (c) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA): Left) DAPI (nuclei, blue), NEUROD1 (neuronal cell, green), <t>DNP63</t> (basal cell, yellow) and KRT8 (luminal basal cell, red). Positive control (+) for NEUROD1 is an RPM olfactory neuroblastoma tumour and for basal markers is an SNL GEMM lung tumour . Right) DAPI (nuclei, blue), FOXJ1 (ciliated cell, purple), CCSP (SCGB1A1, club cell, green) and KRT8 (luminal basal cell, red). Positive control (+) control for FOXJ1 and CCSP is airway from a normal mouse lung, and for KRT8 is an SNL GEMM lung tumour. (d) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA) for DAPI (nuclei, blue) and POU2F3 (tuft cell, green). Positive control (+) is an RPMA olfactory neuroblastoma tumour . All co-IF scale bars=150 μm.
Anti Dnp63, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti dnp63  (Danaher Inc)
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Danaher Inc rabbit anti dnp63
(a) Recombination PCR for RPM basal organoids for indicated alleles pre- and post-treatment with TAT-Cre recombinase or Ad-CMV-Cre at two concentrations (2.5e7 or 5e7 pfu). *Organoids subject to spinoculation with CMV-Cre virus. Red font indicates condition used for subsequent allografting. (b) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA) for DAPI (nuclei, blue), KI67 (proliferation, orange), and ASCL1 (NE cell, green). Positive controls for KI67 and ASCL1 (bottom panel) is an RPR2 SCLC lung tumour. Quantification via CellProfiler of KI67 positivity per organoid from indicated conditions (right). Number of organoids quantified per group is labeled. One-way ANOVA with Tukey’s correction. *** p<0.003, ** p<0.005, ns=not significant, p>0.05. Error bars represent mean ± SD. (c) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA): Left) DAPI (nuclei, blue), NEUROD1 (neuronal cell, green), <t>DNP63</t> (basal cell, yellow) and KRT8 (luminal basal cell, red). Positive control (+) for NEUROD1 is an RPM olfactory neuroblastoma tumour and for basal markers is an SNL GEMM lung tumour . Right) DAPI (nuclei, blue), FOXJ1 (ciliated cell, purple), CCSP (SCGB1A1, club cell, green) and KRT8 (luminal basal cell, red). Positive control (+) control for FOXJ1 and CCSP is airway from a normal mouse lung, and for KRT8 is an SNL GEMM lung tumour. (d) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA) for DAPI (nuclei, blue) and POU2F3 (tuft cell, green). Positive control (+) is an RPMA olfactory neuroblastoma tumour . All co-IF scale bars=150 μm.
Rabbit Anti Dnp63, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dnp63 alpha  (Addgene inc)
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(a) Recombination PCR for RPM basal organoids for indicated alleles pre- and post-treatment with TAT-Cre recombinase or Ad-CMV-Cre at two concentrations (2.5e7 or 5e7 pfu). *Organoids subject to spinoculation with CMV-Cre virus. Red font indicates condition used for subsequent allografting. (b) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA) for DAPI (nuclei, blue), KI67 (proliferation, orange), and ASCL1 (NE cell, green). Positive controls for KI67 and ASCL1 (bottom panel) is an RPR2 SCLC lung tumour. Quantification via CellProfiler of KI67 positivity per organoid from indicated conditions (right). Number of organoids quantified per group is labeled. One-way ANOVA with Tukey’s correction. *** p<0.003, ** p<0.005, ns=not significant, p>0.05. Error bars represent mean ± SD. (c) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA): Left) DAPI (nuclei, blue), NEUROD1 (neuronal cell, green), <t>DNP63</t> (basal cell, yellow) and KRT8 (luminal basal cell, red). Positive control (+) for NEUROD1 is an RPM olfactory neuroblastoma tumour and for basal markers is an SNL GEMM lung tumour . Right) DAPI (nuclei, blue), FOXJ1 (ciliated cell, purple), CCSP (SCGB1A1, club cell, green) and KRT8 (luminal basal cell, red). Positive control (+) control for FOXJ1 and CCSP is airway from a normal mouse lung, and for KRT8 is an SNL GEMM lung tumour. (d) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA) for DAPI (nuclei, blue) and POU2F3 (tuft cell, green). Positive control (+) is an RPMA olfactory neuroblastoma tumour . All co-IF scale bars=150 μm.
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dnp63-positive cells  (Federation of European Neuroscience Societies)
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Federation of European Neuroscience Societies dnp63-positive cells
(a) Recombination PCR for RPM basal organoids for indicated alleles pre- and post-treatment with TAT-Cre recombinase or Ad-CMV-Cre at two concentrations (2.5e7 or 5e7 pfu). *Organoids subject to spinoculation with CMV-Cre virus. Red font indicates condition used for subsequent allografting. (b) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA) for DAPI (nuclei, blue), KI67 (proliferation, orange), and ASCL1 (NE cell, green). Positive controls for KI67 and ASCL1 (bottom panel) is an RPR2 SCLC lung tumour. Quantification via CellProfiler of KI67 positivity per organoid from indicated conditions (right). Number of organoids quantified per group is labeled. One-way ANOVA with Tukey’s correction. *** p<0.003, ** p<0.005, ns=not significant, p>0.05. Error bars represent mean ± SD. (c) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA): Left) DAPI (nuclei, blue), NEUROD1 (neuronal cell, green), <t>DNP63</t> (basal cell, yellow) and KRT8 (luminal basal cell, red). Positive control (+) for NEUROD1 is an RPM olfactory neuroblastoma tumour and for basal markers is an SNL GEMM lung tumour . Right) DAPI (nuclei, blue), FOXJ1 (ciliated cell, purple), CCSP (SCGB1A1, club cell, green) and KRT8 (luminal basal cell, red). Positive control (+) control for FOXJ1 and CCSP is airway from a normal mouse lung, and for KRT8 is an SNL GEMM lung tumour. (d) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA) for DAPI (nuclei, blue) and POU2F3 (tuft cell, green). Positive control (+) is an RPMA olfactory neuroblastoma tumour . All co-IF scale bars=150 μm.
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small-interfering rna (sirna) targeting human dnp63 sasi_hs02_00326884  (Merck KGaA)
90
Merck KGaA small-interfering rna (sirna) targeting human dnp63 sasi_hs02_00326884
(a) Recombination PCR for RPM basal organoids for indicated alleles pre- and post-treatment with TAT-Cre recombinase or Ad-CMV-Cre at two concentrations (2.5e7 or 5e7 pfu). *Organoids subject to spinoculation with CMV-Cre virus. Red font indicates condition used for subsequent allografting. (b) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA) for DAPI (nuclei, blue), KI67 (proliferation, orange), and ASCL1 (NE cell, green). Positive controls for KI67 and ASCL1 (bottom panel) is an RPR2 SCLC lung tumour. Quantification via CellProfiler of KI67 positivity per organoid from indicated conditions (right). Number of organoids quantified per group is labeled. One-way ANOVA with Tukey’s correction. *** p<0.003, ** p<0.005, ns=not significant, p>0.05. Error bars represent mean ± SD. (c) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA): Left) DAPI (nuclei, blue), NEUROD1 (neuronal cell, green), <t>DNP63</t> (basal cell, yellow) and KRT8 (luminal basal cell, red). Positive control (+) for NEUROD1 is an RPM olfactory neuroblastoma tumour and for basal markers is an SNL GEMM lung tumour . Right) DAPI (nuclei, blue), FOXJ1 (ciliated cell, purple), CCSP (SCGB1A1, club cell, green) and KRT8 (luminal basal cell, red). Positive control (+) control for FOXJ1 and CCSP is airway from a normal mouse lung, and for KRT8 is an SNL GEMM lung tumour. (d) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA) for DAPI (nuclei, blue) and POU2F3 (tuft cell, green). Positive control (+) is an RPMA olfactory neuroblastoma tumour . All co-IF scale bars=150 μm.
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(a) Recombination PCR for RPM basal organoids for indicated alleles pre- and post-treatment with TAT-Cre recombinase or Ad-CMV-Cre at two concentrations (2.5e7 or 5e7 pfu). *Organoids subject to spinoculation with CMV-Cre virus. Red font indicates condition used for subsequent allografting. (b) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA) for DAPI (nuclei, blue), KI67 (proliferation, orange), and ASCL1 (NE cell, green). Positive controls for KI67 and ASCL1 (bottom panel) is an RPR2 SCLC lung tumour. Quantification via CellProfiler of KI67 positivity per organoid from indicated conditions (right). Number of organoids quantified per group is labeled. One-way ANOVA with Tukey’s correction. *** p<0.003, ** p<0.005, ns=not significant, p>0.05. Error bars represent mean ± SD. (c) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA): Left) DAPI (nuclei, blue), NEUROD1 (neuronal cell, green), DNP63 (basal cell, yellow) and KRT8 (luminal basal cell, red). Positive control (+) for NEUROD1 is an RPM olfactory neuroblastoma tumour and for basal markers is an SNL GEMM lung tumour . Right) DAPI (nuclei, blue), FOXJ1 (ciliated cell, purple), CCSP (SCGB1A1, club cell, green) and KRT8 (luminal basal cell, red). Positive control (+) control for FOXJ1 and CCSP is airway from a normal mouse lung, and for KRT8 is an SNL GEMM lung tumour. (d) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA) for DAPI (nuclei, blue) and POU2F3 (tuft cell, green). Positive control (+) is an RPMA olfactory neuroblastoma tumour . All co-IF scale bars=150 μm.

Journal: bioRxiv

Article Title: Basal cell of origin resolves neuroendocrine–tuft lineage plasticity in cancer

doi: 10.1101/2024.11.13.623500

Figure Lengend Snippet: (a) Recombination PCR for RPM basal organoids for indicated alleles pre- and post-treatment with TAT-Cre recombinase or Ad-CMV-Cre at two concentrations (2.5e7 or 5e7 pfu). *Organoids subject to spinoculation with CMV-Cre virus. Red font indicates condition used for subsequent allografting. (b) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA) for DAPI (nuclei, blue), KI67 (proliferation, orange), and ASCL1 (NE cell, green). Positive controls for KI67 and ASCL1 (bottom panel) is an RPR2 SCLC lung tumour. Quantification via CellProfiler of KI67 positivity per organoid from indicated conditions (right). Number of organoids quantified per group is labeled. One-way ANOVA with Tukey’s correction. *** p<0.003, ** p<0.005, ns=not significant, p>0.05. Error bars represent mean ± SD. (c) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA): Left) DAPI (nuclei, blue), NEUROD1 (neuronal cell, green), DNP63 (basal cell, yellow) and KRT8 (luminal basal cell, red). Positive control (+) for NEUROD1 is an RPM olfactory neuroblastoma tumour and for basal markers is an SNL GEMM lung tumour . Right) DAPI (nuclei, blue), FOXJ1 (ciliated cell, purple), CCSP (SCGB1A1, club cell, green) and KRT8 (luminal basal cell, red). Positive control (+) control for FOXJ1 and CCSP is airway from a normal mouse lung, and for KRT8 is an SNL GEMM lung tumour. (d) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA) for DAPI (nuclei, blue) and POU2F3 (tuft cell, green). Positive control (+) is an RPMA olfactory neuroblastoma tumour . All co-IF scale bars=150 μm.

Article Snippet: Primary antibodies included: anti-mouse ASCL1 (BD Pharmingen cat#556604) 1:25; anti-rabbit ASCL1 (Abcam cat#211327) 1:100; anti-goat NEUROD1 (R&D Systems cat# AF2746) 1:50; anti-rabbit NEUROD1 (Abcam cat#109224) 1:200; POU2F3 (Sigma cat# HPA019652) 1:100; anti-rabbit CCSP/SCGB1A1 (Millipore Sigma cat#07-623) 1:75; anti-rat KRT8 (DSHB cat# TROMA-I) 1:100; anti-mouse FOXJ1 (eBioscience cat#14-9965-80) 1:100; anti-mouse KI67 (BD Pharmingen cat# BDB556003) 1:100; anti-goat DNP63 (R&D cat# AF1916) 1:40.

Techniques: Virus, Labeling, Positive Control, Control

(a) Recombination PCR for RPM basal organoids for indicated alleles pre- and post-treatment with TAT-Cre recombinase or Ad-CMV-Cre at two concentrations (2.5e7 or 5e7 pfu). *Organoids subject to spinoculation with CMV-Cre virus. Red font indicates condition used for subsequent allografting. (b) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA) for DAPI (nuclei, blue), KI67 (proliferation, orange), and ASCL1 (NE cell, green). Positive controls for KI67 and ASCL1 (bottom panel) is an RPR2 SCLC lung tumour. Quantification via CellProfiler of KI67 positivity per organoid from indicated conditions (right). Number of organoids quantified per group is labeled. One-way ANOVA with Tukey’s correction. *** p<0.003, ** p<0.005, ns=not significant, p>0.05. Error bars represent mean ± SD. (c) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA): Left) DAPI (nuclei, blue), NEUROD1 (neuronal cell, green), DNP63 (basal cell, yellow) and KRT8 (luminal basal cell, red). Positive control (+) for NEUROD1 is an RPM olfactory neuroblastoma tumour and for basal markers is an SNL GEMM lung tumour . Right) DAPI (nuclei, blue), FOXJ1 (ciliated cell, purple), CCSP (SCGB1A1, club cell, green) and KRT8 (luminal basal cell, red). Positive control (+) control for FOXJ1 and CCSP is airway from a normal mouse lung, and for KRT8 is an SNL GEMM lung tumour. (d) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA) for DAPI (nuclei, blue) and POU2F3 (tuft cell, green). Positive control (+) is an RPMA olfactory neuroblastoma tumour . All co-IF scale bars=150 μm.

Journal: bioRxiv

Article Title: Basal cell of origin resolves neuroendocrine–tuft lineage plasticity in cancer

doi: 10.1101/2024.11.13.623500

Figure Lengend Snippet: (a) Recombination PCR for RPM basal organoids for indicated alleles pre- and post-treatment with TAT-Cre recombinase or Ad-CMV-Cre at two concentrations (2.5e7 or 5e7 pfu). *Organoids subject to spinoculation with CMV-Cre virus. Red font indicates condition used for subsequent allografting. (b) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA) for DAPI (nuclei, blue), KI67 (proliferation, orange), and ASCL1 (NE cell, green). Positive controls for KI67 and ASCL1 (bottom panel) is an RPR2 SCLC lung tumour. Quantification via CellProfiler of KI67 positivity per organoid from indicated conditions (right). Number of organoids quantified per group is labeled. One-way ANOVA with Tukey’s correction. *** p<0.003, ** p<0.005, ns=not significant, p>0.05. Error bars represent mean ± SD. (c) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA): Left) DAPI (nuclei, blue), NEUROD1 (neuronal cell, green), DNP63 (basal cell, yellow) and KRT8 (luminal basal cell, red). Positive control (+) for NEUROD1 is an RPM olfactory neuroblastoma tumour and for basal markers is an SNL GEMM lung tumour . Right) DAPI (nuclei, blue), FOXJ1 (ciliated cell, purple), CCSP (SCGB1A1, club cell, green) and KRT8 (luminal basal cell, red). Positive control (+) control for FOXJ1 and CCSP is airway from a normal mouse lung, and for KRT8 is an SNL GEMM lung tumour. (d) Co-IF on basal organoids pre-treatment with Cre (No Cre) or following recombination (RPM, RPR2, RPMA) for DAPI (nuclei, blue) and POU2F3 (tuft cell, green). Positive control (+) is an RPMA olfactory neuroblastoma tumour . All co-IF scale bars=150 μm.

Article Snippet: The primary antibodies include: ASCL1 (Abcam cat#211327) 1:300; NEUROD1 (Abcam cat#109224) 1:300; POU2F3 (Sigma cat# HPA019652) 1:300; YAP1 (CST cat#14074) 1:300; HES1 (CST cat#11988) 1:300; DNP63 (R&D cat# AF1916) 1:400; phospho-AKT Ser473 (CST cat#4060) 1:100; NKX2-1 (Abcam cat# ab76013) 1:250; and KRT5 (BioLegend cat#905501) 1:1000.

Techniques: Virus, Labeling, Positive Control, Control