dnasei  (Worthington Biochemical)


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  • 99
    Name:
    Deoxyribonuclease I
    Description:
    Chromatographically purified A lyophilized powder with glycine as a stabilizer
    Catalog Number:
    LS002004
    Price:
    33
    Source:
    Bovine Pancreas
    Size:
    5 mg
    Cas Number:
    9003.98.9
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    Structured Review

    Worthington Biochemical dnasei
    Definition of an HL-specific active chromatin landscape. ( A ) Experimental strategy. ( B ) Screenshots of the CCL5 ( Left ) and CD79B ( Right ) loci depicting <t>DNaseI-Seq</t> profiles of <t>HRS</t> (red) and NH (green) cell lines. Underneath, microarray-based gene expression data for the respective genes. ( C ) HRS cell lines show similar patterns of distal DHSs. The L1236 over Reh DNaseI-Seq fold change (FC) sorted by increasing values and corresponding signals from comparisons L428 or L591 over Reh, respectively, are shown. Spearman rank correlation coefficients compared with L1236/Reh FCs were 0.82 for both L428/Reh and L591/Reh, respectively. Far right, comparison using the ENCODE lymphoblastoid GM12878 DNaseI-Seq dataset showed a similar result ( r = 0.87). ( D ) Classification of HRS- and NH-specific distal DHSs showing the L1236 over Reh DNaseI-Seq FC (left heat map). Bar next to the heat map: NH (green; group A) and HRS (red; group C) -specific DHSs, respectively. Nonvarying DHSs: black (group B). Panels next to the bar: average DNaseI-Seq profiles for each group. Outermost right, box plot demonstrating the significance of differences in DNaseI-Seq FC for NH- and HRS- specific DHSs (*** P
    Chromatographically purified A lyophilized powder with glycine as a stabilizer
    https://www.bioz.com/result/dnasei/product/Worthington Biochemical
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dnasei - by Bioz Stars, 2021-04
    99/100 stars

    Images

    1) Product Images from "Mapping of transcription factor motifs in active chromatin identifies IRF5 as key regulator in classical Hodgkin lymphoma"

    Article Title: Mapping of transcription factor motifs in active chromatin identifies IRF5 as key regulator in classical Hodgkin lymphoma

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.1406985111

    Definition of an HL-specific active chromatin landscape. ( A ) Experimental strategy. ( B ) Screenshots of the CCL5 ( Left ) and CD79B ( Right ) loci depicting DNaseI-Seq profiles of HRS (red) and NH (green) cell lines. Underneath, microarray-based gene expression data for the respective genes. ( C ) HRS cell lines show similar patterns of distal DHSs. The L1236 over Reh DNaseI-Seq fold change (FC) sorted by increasing values and corresponding signals from comparisons L428 or L591 over Reh, respectively, are shown. Spearman rank correlation coefficients compared with L1236/Reh FCs were 0.82 for both L428/Reh and L591/Reh, respectively. Far right, comparison using the ENCODE lymphoblastoid GM12878 DNaseI-Seq dataset showed a similar result ( r = 0.87). ( D ) Classification of HRS- and NH-specific distal DHSs showing the L1236 over Reh DNaseI-Seq FC (left heat map). Bar next to the heat map: NH (green; group A) and HRS (red; group C) -specific DHSs, respectively. Nonvarying DHSs: black (group B). Panels next to the bar: average DNaseI-Seq profiles for each group. Outermost right, box plot demonstrating the significance of differences in DNaseI-Seq FC for NH- and HRS- specific DHSs (*** P
    Figure Legend Snippet: Definition of an HL-specific active chromatin landscape. ( A ) Experimental strategy. ( B ) Screenshots of the CCL5 ( Left ) and CD79B ( Right ) loci depicting DNaseI-Seq profiles of HRS (red) and NH (green) cell lines. Underneath, microarray-based gene expression data for the respective genes. ( C ) HRS cell lines show similar patterns of distal DHSs. The L1236 over Reh DNaseI-Seq fold change (FC) sorted by increasing values and corresponding signals from comparisons L428 or L591 over Reh, respectively, are shown. Spearman rank correlation coefficients compared with L1236/Reh FCs were 0.82 for both L428/Reh and L591/Reh, respectively. Far right, comparison using the ENCODE lymphoblastoid GM12878 DNaseI-Seq dataset showed a similar result ( r = 0.87). ( D ) Classification of HRS- and NH-specific distal DHSs showing the L1236 over Reh DNaseI-Seq FC (left heat map). Bar next to the heat map: NH (green; group A) and HRS (red; group C) -specific DHSs, respectively. Nonvarying DHSs: black (group B). Panels next to the bar: average DNaseI-Seq profiles for each group. Outermost right, box plot demonstrating the significance of differences in DNaseI-Seq FC for NH- and HRS- specific DHSs (*** P

    Techniques Used: Microarray, Expressing

    Related Articles

    Concentration Assay:

    Article Title: NuMA Influences Higher Order Chromatin Organization in Human Mammary Epithelium
    Article Snippet: .. DNase I (Worthington Biochemical) was added to a final concentration of 130 μg/ml. .. To aid the removal of cut DNA, (NH4 )2 SO4 was added to a final concentration of 0.25 M and the samples were incubated for 5 min at room temperature.

    Article Title: STAT3, STAT4, NFATc1, and CTCF regulate PD-1 through multiple novel regulatory regions in murine T cells
    Article Snippet: Briefly, nuclei were isolated and treated with increasing concentrations of DNase I (Worthington Biochemical Co, Lakewood, NJ). .. Concentration ranges for DNase I were determined empirically for each lot and cell type by titrating DNase I for its ability to digest CR-C as the positive control but not regions previously found to be resistant to DNase I (e.g., +6.3 region). .. Following purification of the digested DNA, PCR was performed across the Pdcd1 locus using a set of 59 primer pairs ( ).

    other:

    Article Title: DNase I aggravates islet β-cell apoptosis in type 2 diabetes
    Article Snippet: DNase I is ubiquitously expressed in mammalian tissues, particularly in the pancreas ( ).

    Expressing:

    Article Title: DNase I aggravates islet β-cell apoptosis in type 2 diabetes
    Article Snippet: In summary, the present study provided novel insight into the central role of DNase I in high glucose-induced pancreatic β-cell apoptosis. .. It was demonstrated that high glucose was able to increase the expression of DNase I, and that this may aggravate β-cell apoptosis. ..

    End Labeling:

    Article Title: Nucleosomes Are Translationally Positioned on the Active Allele and Rotationally Positioned on the Inactive Allele of the HPRT Promoter
    Article Snippet: The positions of the MNase cleavages within chromatin of the HPRT promoter region of permeabilized cells relative to a downstream Bcl I site in the first intron of the HPRT gene were mapped using a 400-bp hybridization probe located just upstream of the Bcl I site (Fig. ). .. To determine the positions of DNase I-hypersensitive sites relative to MNase cleavage sites, NP-40-permeabilized cells containing the active HPRT allele were treated with increasing concentrations of DNase I and the DNase I-hypersensitive sites in chromatin of the HPRT promoter relative to the same Bcl I site were also mapped by indirect end labeling using the same hybridization probe. .. Figure shows the Southern blot analysis of the DNase I and MNase cleavage patterns on the inactive and active HPRT promoters in permeabilized 8121 and 4.12 cells, respectively.

    Hybridization:

    Article Title: Nucleosomes Are Translationally Positioned on the Active Allele and Rotationally Positioned on the Inactive Allele of the HPRT Promoter
    Article Snippet: The positions of the MNase cleavages within chromatin of the HPRT promoter region of permeabilized cells relative to a downstream Bcl I site in the first intron of the HPRT gene were mapped using a 400-bp hybridization probe located just upstream of the Bcl I site (Fig. ). .. To determine the positions of DNase I-hypersensitive sites relative to MNase cleavage sites, NP-40-permeabilized cells containing the active HPRT allele were treated with increasing concentrations of DNase I and the DNase I-hypersensitive sites in chromatin of the HPRT promoter relative to the same Bcl I site were also mapped by indirect end labeling using the same hybridization probe. .. Figure shows the Southern blot analysis of the DNase I and MNase cleavage patterns on the inactive and active HPRT promoters in permeabilized 8121 and 4.12 cells, respectively.

    Cell Culture:

    Article Title: Evaluation of the Functional Involvement of Human Immunodeficiency Virus Type 1 Integrase in Nuclear Import of Viral cDNA during Acute Infection
    Article Snippet: Pseudotype viruses were prepared by cotransfection of 293T cells with the pNL43lucΔenv vector together with the MuLV envelope expression vector (pJD-1). .. After treatment with DNase I (40 μg/ml; Worthington), each virus (70 ng of p24) was inoculated into HeLa cells and cultured at 37°C for 6 h. The cells were washed with PBS and resuspended in fresh medium (DMEM plus 10% FBS). ..

    Lysis:

    Article Title: Preparation of Soluble Proteins from Escherichia coli
    Article Snippet: The purification process time described can be shortened through the use of chromatography systems and fast-flow column matrices (see and Time Considerations). .. DEAE Sepharose CL-4B resin GE Heathcare Life Sciences) Anion-exchange buffer (see recipe) 0.26% (w/v) sodium hypochlorite/70% ethanol or 5% (v/v) bleach (e.g., Clorox)/70% ethanol E. coli cells (~50 g wet weight) from fermentation ( UNIT 5.3 ) containing IL-1β Lysis buffer (see recipe) Bovine pancreas DNase I and RNase A (Worthington Biochemical; optional, for reducing solution viscosity) 2 N sodium hydroxide Ammonium sulfate, ground with mortar and pestle Cation-exchange buffer (see recipe) CM Sepharose CL-4B (GE Heathcare Cation-exchange buffer/250 mM NaCl (see recipe) Tris base Gel-filtration buffer (see recipe) Ultrogel AcA54 gel-permeation resin (Pall Corporation; Sigma-Aldrich)) Lyophilization buffer (see recipe; optional) 2- or 3-liter sintered glass funnel with fritted disc (coarse porosity) and 5-liter filter flask Chromatography columns (preferably glass) with adjustable flow adapters: one (or optionally two) 5 × 50 cm and one 2.5 × 100 cm (GE Healthcare), Amicon, or equivalent) RK50 packing reservoir GE Heathcare) Peristaltic pump, UV monitor, and fraction collector (GE Heathcare; check on-line for other suppliers of equivalent items) 16 × 150–mm culture tubes 40-ml French pressure cell and rapid-fill kit (Thermo Fisher Scientific – see Commentary section on breaking cells).) .. French laboratory press (Thermo Fisher Scientific)) 1-liter Waring commercial blender.

    Chromatography:

    Article Title: Preparation of Soluble Proteins from Escherichia coli
    Article Snippet: The purification process time described can be shortened through the use of chromatography systems and fast-flow column matrices (see and Time Considerations). .. DEAE Sepharose CL-4B resin GE Heathcare Life Sciences) Anion-exchange buffer (see recipe) 0.26% (w/v) sodium hypochlorite/70% ethanol or 5% (v/v) bleach (e.g., Clorox)/70% ethanol E. coli cells (~50 g wet weight) from fermentation ( UNIT 5.3 ) containing IL-1β Lysis buffer (see recipe) Bovine pancreas DNase I and RNase A (Worthington Biochemical; optional, for reducing solution viscosity) 2 N sodium hydroxide Ammonium sulfate, ground with mortar and pestle Cation-exchange buffer (see recipe) CM Sepharose CL-4B (GE Heathcare Cation-exchange buffer/250 mM NaCl (see recipe) Tris base Gel-filtration buffer (see recipe) Ultrogel AcA54 gel-permeation resin (Pall Corporation; Sigma-Aldrich)) Lyophilization buffer (see recipe; optional) 2- or 3-liter sintered glass funnel with fritted disc (coarse porosity) and 5-liter filter flask Chromatography columns (preferably glass) with adjustable flow adapters: one (or optionally two) 5 × 50 cm and one 2.5 × 100 cm (GE Healthcare), Amicon, or equivalent) RK50 packing reservoir GE Heathcare) Peristaltic pump, UV monitor, and fraction collector (GE Heathcare; check on-line for other suppliers of equivalent items) 16 × 150–mm culture tubes 40-ml French pressure cell and rapid-fill kit (Thermo Fisher Scientific – see Commentary section on breaking cells).) .. French laboratory press (Thermo Fisher Scientific)) 1-liter Waring commercial blender.

    Flow Cytometry:

    Article Title: Preparation of Soluble Proteins from Escherichia coli
    Article Snippet: The purification process time described can be shortened through the use of chromatography systems and fast-flow column matrices (see and Time Considerations). .. DEAE Sepharose CL-4B resin GE Heathcare Life Sciences) Anion-exchange buffer (see recipe) 0.26% (w/v) sodium hypochlorite/70% ethanol or 5% (v/v) bleach (e.g., Clorox)/70% ethanol E. coli cells (~50 g wet weight) from fermentation ( UNIT 5.3 ) containing IL-1β Lysis buffer (see recipe) Bovine pancreas DNase I and RNase A (Worthington Biochemical; optional, for reducing solution viscosity) 2 N sodium hydroxide Ammonium sulfate, ground with mortar and pestle Cation-exchange buffer (see recipe) CM Sepharose CL-4B (GE Heathcare Cation-exchange buffer/250 mM NaCl (see recipe) Tris base Gel-filtration buffer (see recipe) Ultrogel AcA54 gel-permeation resin (Pall Corporation; Sigma-Aldrich)) Lyophilization buffer (see recipe; optional) 2- or 3-liter sintered glass funnel with fritted disc (coarse porosity) and 5-liter filter flask Chromatography columns (preferably glass) with adjustable flow adapters: one (or optionally two) 5 × 50 cm and one 2.5 × 100 cm (GE Healthcare), Amicon, or equivalent) RK50 packing reservoir GE Heathcare) Peristaltic pump, UV monitor, and fraction collector (GE Heathcare; check on-line for other suppliers of equivalent items) 16 × 150–mm culture tubes 40-ml French pressure cell and rapid-fill kit (Thermo Fisher Scientific – see Commentary section on breaking cells).) .. French laboratory press (Thermo Fisher Scientific)) 1-liter Waring commercial blender.

    Positive Control:

    Article Title: STAT3, STAT4, NFATc1, and CTCF regulate PD-1 through multiple novel regulatory regions in murine T cells
    Article Snippet: Briefly, nuclei were isolated and treated with increasing concentrations of DNase I (Worthington Biochemical Co, Lakewood, NJ). .. Concentration ranges for DNase I were determined empirically for each lot and cell type by titrating DNase I for its ability to digest CR-C as the positive control but not regions previously found to be resistant to DNase I (e.g., +6.3 region). .. Following purification of the digested DNA, PCR was performed across the Pdcd1 locus using a set of 59 primer pairs ( ).

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  • 99
    Worthington Biochemical dnasei
    Definition of an HL-specific active chromatin landscape. ( A ) Experimental strategy. ( B ) Screenshots of the CCL5 ( Left ) and CD79B ( Right ) loci depicting <t>DNaseI-Seq</t> profiles of <t>HRS</t> (red) and NH (green) cell lines. Underneath, microarray-based gene expression data for the respective genes. ( C ) HRS cell lines show similar patterns of distal DHSs. The L1236 over Reh DNaseI-Seq fold change (FC) sorted by increasing values and corresponding signals from comparisons L428 or L591 over Reh, respectively, are shown. Spearman rank correlation coefficients compared with L1236/Reh FCs were 0.82 for both L428/Reh and L591/Reh, respectively. Far right, comparison using the ENCODE lymphoblastoid GM12878 DNaseI-Seq dataset showed a similar result ( r = 0.87). ( D ) Classification of HRS- and NH-specific distal DHSs showing the L1236 over Reh DNaseI-Seq FC (left heat map). Bar next to the heat map: NH (green; group A) and HRS (red; group C) -specific DHSs, respectively. Nonvarying DHSs: black (group B). Panels next to the bar: average DNaseI-Seq profiles for each group. Outermost right, box plot demonstrating the significance of differences in DNaseI-Seq FC for NH- and HRS- specific DHSs (*** P
    Dnasei, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnasei/product/Worthington Biochemical
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dnasei - by Bioz Stars, 2021-04
    99/100 stars
      Buy from Supplier

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    Definition of an HL-specific active chromatin landscape. ( A ) Experimental strategy. ( B ) Screenshots of the CCL5 ( Left ) and CD79B ( Right ) loci depicting DNaseI-Seq profiles of HRS (red) and NH (green) cell lines. Underneath, microarray-based gene expression data for the respective genes. ( C ) HRS cell lines show similar patterns of distal DHSs. The L1236 over Reh DNaseI-Seq fold change (FC) sorted by increasing values and corresponding signals from comparisons L428 or L591 over Reh, respectively, are shown. Spearman rank correlation coefficients compared with L1236/Reh FCs were 0.82 for both L428/Reh and L591/Reh, respectively. Far right, comparison using the ENCODE lymphoblastoid GM12878 DNaseI-Seq dataset showed a similar result ( r = 0.87). ( D ) Classification of HRS- and NH-specific distal DHSs showing the L1236 over Reh DNaseI-Seq FC (left heat map). Bar next to the heat map: NH (green; group A) and HRS (red; group C) -specific DHSs, respectively. Nonvarying DHSs: black (group B). Panels next to the bar: average DNaseI-Seq profiles for each group. Outermost right, box plot demonstrating the significance of differences in DNaseI-Seq FC for NH- and HRS- specific DHSs (*** P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Mapping of transcription factor motifs in active chromatin identifies IRF5 as key regulator in classical Hodgkin lymphoma

    doi: 10.1073/pnas.1406985111

    Figure Lengend Snippet: Definition of an HL-specific active chromatin landscape. ( A ) Experimental strategy. ( B ) Screenshots of the CCL5 ( Left ) and CD79B ( Right ) loci depicting DNaseI-Seq profiles of HRS (red) and NH (green) cell lines. Underneath, microarray-based gene expression data for the respective genes. ( C ) HRS cell lines show similar patterns of distal DHSs. The L1236 over Reh DNaseI-Seq fold change (FC) sorted by increasing values and corresponding signals from comparisons L428 or L591 over Reh, respectively, are shown. Spearman rank correlation coefficients compared with L1236/Reh FCs were 0.82 for both L428/Reh and L591/Reh, respectively. Far right, comparison using the ENCODE lymphoblastoid GM12878 DNaseI-Seq dataset showed a similar result ( r = 0.87). ( D ) Classification of HRS- and NH-specific distal DHSs showing the L1236 over Reh DNaseI-Seq FC (left heat map). Bar next to the heat map: NH (green; group A) and HRS (red; group C) -specific DHSs, respectively. Nonvarying DHSs: black (group B). Panels next to the bar: average DNaseI-Seq profiles for each group. Outermost right, box plot demonstrating the significance of differences in DNaseI-Seq FC for NH- and HRS- specific DHSs (*** P

    Article Snippet: To perform DNaseI accessibility assays with HRS and NH cell lines, the optimal cell number and DNaseI (DPFF DNaseI; Worthington Biochemical Corporation) concentration were titrated for each cell line.

    Techniques: Microarray, Expressing