dnasei  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    DNase I RNase free
    Description:
    DNase I RNase free 5 000 units
    Catalog Number:
    M0303L
    Price:
    282
    Category:
    Deoxyribonucleases DNase
    Size:
    5 000 units
    Buy from Supplier


    Structured Review

    New England Biolabs dnasei
    DNase I RNase free
    DNase I RNase free 5 000 units
    https://www.bioz.com/result/dnasei/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dnasei - by Bioz Stars, 2021-04
    99/100 stars

    Images

    1) Product Images from "Integrin α4β1 controls G9a activity that regulates epigenetic changes and nuclear properties required for lymphocyte migration"

    Article Title: Integrin α4β1 controls G9a activity that regulates epigenetic changes and nuclear properties required for lymphocyte migration

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkv1348

    Cell adhesion through α4β1 regulates global chromatin conformation. ( A ) Jurkat cells were cultured on poly-Lysine, ICAM1 or VCAM1 for 24 h. Cytoskeleton was removed by extensive CSK buffer washes and DNA was digested by DNaseI before fixation. Remaining DNA was stained with Hoechst. Bar 10 μm. ( B ) Graphs show the quantification of the nuclear volume and area from cells in (A). ( C ) Jurkat (left panel) or primary CD4 + T-cells (right panel) were cultured in suspension, on ICAM1 and VCAM1 for 24 h. Then, cells were digested with micrococcal nuclease at indicated times. DNA fragments were purified and resolved in agarose gel. ( D ) Nucleosomal releasing from cells in (C) was analysed after micrococcal digestion and the mononucleosomes (1n), dinuclueosomes (2n) and trinucleosomes (3n) quantified. * P
    Figure Legend Snippet: Cell adhesion through α4β1 regulates global chromatin conformation. ( A ) Jurkat cells were cultured on poly-Lysine, ICAM1 or VCAM1 for 24 h. Cytoskeleton was removed by extensive CSK buffer washes and DNA was digested by DNaseI before fixation. Remaining DNA was stained with Hoechst. Bar 10 μm. ( B ) Graphs show the quantification of the nuclear volume and area from cells in (A). ( C ) Jurkat (left panel) or primary CD4 + T-cells (right panel) were cultured in suspension, on ICAM1 and VCAM1 for 24 h. Then, cells were digested with micrococcal nuclease at indicated times. DNA fragments were purified and resolved in agarose gel. ( D ) Nucleosomal releasing from cells in (C) was analysed after micrococcal digestion and the mononucleosomes (1n), dinuclueosomes (2n) and trinucleosomes (3n) quantified. * P

    Techniques Used: Cell Culture, Staining, Purification, Agarose Gel Electrophoresis

    Related Articles

    Footprinting:

    Article Title: The crystal structure of the TetR family transcriptional repressor SimR bound to DNA and the role of a flexible N-terminal extension in minor groove binding
    Article Snippet: After incubation at 22°C for 10 min, the binding reaction mixtures were loaded on 5% (w/v) native polyacrylamide gels and run in TBE buffer at 100 V for 45 min. EMSA data were collected and analysed on a PhosphoImager (FujiFilm) using Multi Gauge image analysis software (FujiFilm). .. DNase I footprinting Templates for DNase I footprinting were amplified by PCR using one unlabelled primer and one primer 5′-end labelled using [γ32 -P] ATP and T4 polynucleotide kinase (New England Biolabs). ..

    Amplification:

    Article Title: The crystal structure of the TetR family transcriptional repressor SimR bound to DNA and the role of a flexible N-terminal extension in minor groove binding
    Article Snippet: After incubation at 22°C for 10 min, the binding reaction mixtures were loaded on 5% (w/v) native polyacrylamide gels and run in TBE buffer at 100 V for 45 min. EMSA data were collected and analysed on a PhosphoImager (FujiFilm) using Multi Gauge image analysis software (FujiFilm). .. DNase I footprinting Templates for DNase I footprinting were amplified by PCR using one unlabelled primer and one primer 5′-end labelled using [γ32 -P] ATP and T4 polynucleotide kinase (New England Biolabs). ..

    Polymerase Chain Reaction:

    Article Title: The crystal structure of the TetR family transcriptional repressor SimR bound to DNA and the role of a flexible N-terminal extension in minor groove binding
    Article Snippet: After incubation at 22°C for 10 min, the binding reaction mixtures were loaded on 5% (w/v) native polyacrylamide gels and run in TBE buffer at 100 V for 45 min. EMSA data were collected and analysed on a PhosphoImager (FujiFilm) using Multi Gauge image analysis software (FujiFilm). .. DNase I footprinting Templates for DNase I footprinting were amplified by PCR using one unlabelled primer and one primer 5′-end labelled using [γ32 -P] ATP and T4 polynucleotide kinase (New England Biolabs). ..

    Lysis:

    Article Title: Detecting RNA-RNA interactions in E. coli using a modified CLASH method
    Article Snippet: Identification of ligated RNAs This study (Fig. ) employed in vivo crosslinking of RNA duplexes with the AMT molecule, which can, upon 365 nm UV irradiation, generate inter-strand adducts between juxtaposed uridine bases [ ]. .. Following cell lysis and RNA extraction, DNA residues were digested by DNase I, and single strand RNAs and free RNA overhangs adjacent to duplexes were digested by RNase T1. .. Then, the residual single strand RNAs were hybridized with 20 nt oligonucleotides and digested by RNase H three times.

    RNA Extraction:

    Article Title: Detecting RNA-RNA interactions in E. coli using a modified CLASH method
    Article Snippet: Identification of ligated RNAs This study (Fig. ) employed in vivo crosslinking of RNA duplexes with the AMT molecule, which can, upon 365 nm UV irradiation, generate inter-strand adducts between juxtaposed uridine bases [ ]. .. Following cell lysis and RNA extraction, DNA residues were digested by DNase I, and single strand RNAs and free RNA overhangs adjacent to duplexes were digested by RNase T1. .. Then, the residual single strand RNAs were hybridized with 20 nt oligonucleotides and digested by RNase H three times.

    Binding Assay:

    Article Title: Evidence that Altered Cis Element Spacing Affects PpsR Mediated Redox Control of Photosynthesis Gene Expression in Rubrivivax gelatinosus
    Article Snippet: Individual footprint reactions were initiated with a 22 μl binding reaction mixture that contained 12.5 mM HEPES (pH 7.8), 5 mM K-acetate (pH 8.0), 2.5 mM Mg-acetate, 1 mM CaCl2 , 12.5 μg/ml bovine serum albumin [ ], 0.3 mg/ml heparin, 200 nM of fluorescence-labeled DNA probe and various amounts of purified protein. .. Initial binding reaction mixtures were incubated for 30 min at 22°C followed by a 15 min DNase I digestion that was initiated by adding 3 μl of DNase I (New England Biolabs) at an approximately 1:100 dilution (0.02 units/μl) in a footprint binding buffer, which gave partial probe digestion. ..

    Incubation:

    Article Title: Evidence that Altered Cis Element Spacing Affects PpsR Mediated Redox Control of Photosynthesis Gene Expression in Rubrivivax gelatinosus
    Article Snippet: Individual footprint reactions were initiated with a 22 μl binding reaction mixture that contained 12.5 mM HEPES (pH 7.8), 5 mM K-acetate (pH 8.0), 2.5 mM Mg-acetate, 1 mM CaCl2 , 12.5 μg/ml bovine serum albumin [ ], 0.3 mg/ml heparin, 200 nM of fluorescence-labeled DNA probe and various amounts of purified protein. .. Initial binding reaction mixtures were incubated for 30 min at 22°C followed by a 15 min DNase I digestion that was initiated by adding 3 μl of DNase I (New England Biolabs) at an approximately 1:100 dilution (0.02 units/μl) in a footprint binding buffer, which gave partial probe digestion. ..

    Article Title: Intronic Cis-Regulatory Modules Mediate Tissue-Specific and Microbial Control of angptl4/fiaf Transcription
    Article Snippet: .. Nuclei were incubated with various concentrations of Dnase I (0–1.5 units, NEB) for 10 minutes at 37°C. .. Reactions were stopped by adding an equal volume of 2× Lysis Buffer (1% SDS, 200 mM NaCl, 10 mM EDTA, 20 mM Tris pH 7.5, 0.4 mg/ml proteinase K) and incubated overnight at 37°C.

    Synthesized:

    Article Title: Genome-wide characterization of methylguanosine-capped and polyadenylated small RNAs in the rice blast fungus Magnaporthe oryzae
    Article Snippet: The quantity of 5′-methylguanosine-capped RNA was measured by NanoDrop (Thermo Fisher) analysis and its integrity was determined with an Agilent 2100 Bioanalyzer. .. 3′-RACE analysis of CPA-sRNAs using 5′-capped RNA 5′-methylguanosine-capped RNA was treated with DNase I (NEB) to remove any contaminating genomic DNA. cDNA was synthesized in 20 µl reactions by adding the following reagents: 1 µg of 5′-methylguanosine-capped RNA, 50 picomole of 3′-oligo(dT) 20 VN primer, 5 mM of dNTPs, 1 U of RNaseOut (Invitrogen) and 5 U of Superscript III (Invitrogen). ..

    other:

    Article Title: Campylobacter-Induced Interleukin-8 Secretion in Polarized Human Intestinal Epithelial Cells Requires Campylobacter-Secreted Cytolethal Distending Toxin- and Toll-Like Receptor-Mediated Activation of NF-?B ▿
    Article Snippet: However, treatment of the basolateral conditioned supernatant with DNase I did not significantly change its ability to induce IL-8 secretion (Fig. ).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    New England Biolabs dnasei
    Analysis of epigenomic signatures around transcription start site (TSS) of elncRNAs and plncRNAs. (A–D) Average normalized RPKM (reads per kilobase million) values of <t>RNA</t> polII, p300, <t>DNaseI</t> hypersensitivity site, and CCCTC binding factor (CTCF) in elncRNAs and plncRNAs, respectively; (E,F) Contrast chromatin (H3K4Me1 and H3K4Me3) and epigenetic (RNA polII, p300, DNaseI hypersensitivity and CTCF binding site) landscapes (in mouse lungs) around TSS of NONMMUT013718 and NONMMUT024103 elncRNAs, respectively. *** P ≤ 0.001 and ns = non-significant.
    Dnasei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnasei/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dnasei - by Bioz Stars, 2021-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    Analysis of epigenomic signatures around transcription start site (TSS) of elncRNAs and plncRNAs. (A–D) Average normalized RPKM (reads per kilobase million) values of RNA polII, p300, DNaseI hypersensitivity site, and CCCTC binding factor (CTCF) in elncRNAs and plncRNAs, respectively; (E,F) Contrast chromatin (H3K4Me1 and H3K4Me3) and epigenetic (RNA polII, p300, DNaseI hypersensitivity and CTCF binding site) landscapes (in mouse lungs) around TSS of NONMMUT013718 and NONMMUT024103 elncRNAs, respectively. *** P ≤ 0.001 and ns = non-significant.

    Journal: Frontiers in Immunology

    Article Title: Enhancer Associated Long Non-coding RNA Transcription and Gene Regulation in Experimental Models of Rickettsial Infection

    doi: 10.3389/fimmu.2018.03014

    Figure Lengend Snippet: Analysis of epigenomic signatures around transcription start site (TSS) of elncRNAs and plncRNAs. (A–D) Average normalized RPKM (reads per kilobase million) values of RNA polII, p300, DNaseI hypersensitivity site, and CCCTC binding factor (CTCF) in elncRNAs and plncRNAs, respectively; (E,F) Contrast chromatin (H3K4Me1 and H3K4Me3) and epigenetic (RNA polII, p300, DNaseI hypersensitivity and CTCF binding site) landscapes (in mouse lungs) around TSS of NONMMUT013718 and NONMMUT024103 elncRNAs, respectively. *** P ≤ 0.001 and ns = non-significant.

    Article Snippet: RNA samples were subjected to treatment with DNaseI (NEB) to remove any contaminating DNA and then enriched with Ribo-Zero rRNA Removal kit (Illumina).

    Techniques: Binding Assay

    DHAs identify nucleosome-depleted regions flanking Arc . A , Probes used to map hypersensitive sites at the Arc locus. Genomic DNA fragments were detected by Southern blot probes that annealed either immediately upstream (“U”; black bar) or downstream (“D”; black bar) of the SspI restriction site in Arc . Arrows indicate the relative positions of the nine DNaseI HSs (A–I) identified at the Arc locus; gray circles represent DNaseI-inaccessible nucleosome-packaged regions. B , Top, DHAs identify seven discrete “open” regions (bands A–G; arrows) upstream of Arc . Some are present in both rat cortical neurons (RCNs) and PC12 cells (bands A, B, D, F, G); others are visible only in RCNs (band E) or only in PC12 cells (band C). There is a larger open smear (asterisks) at the TSS. The “naked” DNA negative control, performed using both PC12 and RCN DNA, exhibits only bands A and G, suggesting that they are either DHA artifacts or atypical sites and that the remaining bands and smear reflect the native chromatin structure upstream of Arc . Bottom, DHAs identify two discrete open regions (bands H, I) downstream of Arc ). All Southern blot images are representative of at least three experiments. Distances are in kb from the Arc ). PB: primary band resulting from SspI restriction cuts.

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: The Serum Response Factor and a Putative Novel Transcription Factor Regulate Expression of the Immediate-Early Gene Arc/Arg3.1 in Cultured Cortical Neurons

    doi: 10.1523/JNEUROSCI.5575-08.2009

    Figure Lengend Snippet: DHAs identify nucleosome-depleted regions flanking Arc . A , Probes used to map hypersensitive sites at the Arc locus. Genomic DNA fragments were detected by Southern blot probes that annealed either immediately upstream (“U”; black bar) or downstream (“D”; black bar) of the SspI restriction site in Arc . Arrows indicate the relative positions of the nine DNaseI HSs (A–I) identified at the Arc locus; gray circles represent DNaseI-inaccessible nucleosome-packaged regions. B , Top, DHAs identify seven discrete “open” regions (bands A–G; arrows) upstream of Arc . Some are present in both rat cortical neurons (RCNs) and PC12 cells (bands A, B, D, F, G); others are visible only in RCNs (band E) or only in PC12 cells (band C). There is a larger open smear (asterisks) at the TSS. The “naked” DNA negative control, performed using both PC12 and RCN DNA, exhibits only bands A and G, suggesting that they are either DHA artifacts or atypical sites and that the remaining bands and smear reflect the native chromatin structure upstream of Arc . Bottom, DHAs identify two discrete open regions (bands H, I) downstream of Arc ). All Southern blot images are representative of at least three experiments. Distances are in kb from the Arc ). PB: primary band resulting from SspI restriction cuts.

    Article Snippet: Genomic DNA (∼30 μg) was isolated from each DNaseI-treated aliquot of nuclei and digested overnight with SspI (New England Biolabs), a restriction enzyme with cut sites throughout the Arc locus: ∼13.2 kilobases (kb) upstream of, 925 base pairs (bp) downstream of, and ∼19.3 kb downstream of the Arc transcription start site (TSS).

    Techniques: Southern Blot, Negative Control

    Cell adhesion through α4β1 regulates global chromatin conformation. ( A ) Jurkat cells were cultured on poly-Lysine, ICAM1 or VCAM1 for 24 h. Cytoskeleton was removed by extensive CSK buffer washes and DNA was digested by DNaseI before fixation. Remaining DNA was stained with Hoechst. Bar 10 μm. ( B ) Graphs show the quantification of the nuclear volume and area from cells in (A). ( C ) Jurkat (left panel) or primary CD4 + T-cells (right panel) were cultured in suspension, on ICAM1 and VCAM1 for 24 h. Then, cells were digested with micrococcal nuclease at indicated times. DNA fragments were purified and resolved in agarose gel. ( D ) Nucleosomal releasing from cells in (C) was analysed after micrococcal digestion and the mononucleosomes (1n), dinuclueosomes (2n) and trinucleosomes (3n) quantified. * P

    Journal: Nucleic Acids Research

    Article Title: Integrin α4β1 controls G9a activity that regulates epigenetic changes and nuclear properties required for lymphocyte migration

    doi: 10.1093/nar/gkv1348

    Figure Lengend Snippet: Cell adhesion through α4β1 regulates global chromatin conformation. ( A ) Jurkat cells were cultured on poly-Lysine, ICAM1 or VCAM1 for 24 h. Cytoskeleton was removed by extensive CSK buffer washes and DNA was digested by DNaseI before fixation. Remaining DNA was stained with Hoechst. Bar 10 μm. ( B ) Graphs show the quantification of the nuclear volume and area from cells in (A). ( C ) Jurkat (left panel) or primary CD4 + T-cells (right panel) were cultured in suspension, on ICAM1 and VCAM1 for 24 h. Then, cells were digested with micrococcal nuclease at indicated times. DNA fragments were purified and resolved in agarose gel. ( D ) Nucleosomal releasing from cells in (C) was analysed after micrococcal digestion and the mononucleosomes (1n), dinuclueosomes (2n) and trinucleosomes (3n) quantified. * P

    Article Snippet: Briefly, Jurkat cells were cultured on different ligands and lysed in CSK buffer for 5 min and then DNAseI (New England Biolabs; Ipswich, MA, USA) was incorporated for 20 min.

    Techniques: Cell Culture, Staining, Purification, Agarose Gel Electrophoresis

    Analysis of epigenomic signatures around transcription start site (TSS) of elncRNAs and plncRNAs. (A–D) Average normalized RPKM (reads per kilobase million) values of RNA polII, p300, DNaseI hypersensitivity site, and CCCTC binding factor (CTCF) in elncRNAs and plncRNAs, respectively; (E,F) Contrast chromatin (H3K4Me1 and H3K4Me3) and epigenetic (RNA polII, p300, DNaseI hypersensitivity and CTCF binding site) landscapes (in mouse lungs) around TSS of NONMMUT013718 and NONMMUT024103 elncRNAs, respectively. *** P ≤ 0.001 and ns = non-significant.

    Journal: Frontiers in Immunology

    Article Title: Enhancer Associated Long Non-coding RNA Transcription and Gene Regulation in Experimental Models of Rickettsial Infection

    doi: 10.3389/fimmu.2018.03014

    Figure Lengend Snippet: Analysis of epigenomic signatures around transcription start site (TSS) of elncRNAs and plncRNAs. (A–D) Average normalized RPKM (reads per kilobase million) values of RNA polII, p300, DNaseI hypersensitivity site, and CCCTC binding factor (CTCF) in elncRNAs and plncRNAs, respectively; (E,F) Contrast chromatin (H3K4Me1 and H3K4Me3) and epigenetic (RNA polII, p300, DNaseI hypersensitivity and CTCF binding site) landscapes (in mouse lungs) around TSS of NONMMUT013718 and NONMMUT024103 elncRNAs, respectively. *** P ≤ 0.001 and ns = non-significant.

    Article Snippet: RNA samples were subjected to treatment with DNaseI (NEB) to remove any contaminating DNA and then enriched with Ribo-Zero rRNA Removal kit (Illumina).

    Techniques: Binding Assay