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Boehringer Mannheim dnasei
Comparison of in vivo nucleosome positions on the wild-type RPS28A promoter and a promoter containing a mutated T-rich element. Nystatin-permeabilized spheroplasts prepared from wild-type (ERS3) and mutant cells (ETS6) were treated with either <t>Mnase</t> ( A ) or <t>DNaseI</t> ( B ). Distances are from the translational start codon ATG. Protected regions of 160–170 bp are interpreted as positioned nucleosomes (closed elipses). The 130 bp Mnase-protected region that covers the Abf1p site ( Xba I) in the wild-type promoter (ERS3) has decreased to 85 bp when the T-rich element is mutated (ERS6). Concomitantly, downstream nucleosomes are shifted towards the Abf1p binding site.
Dnasei, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Different roles for Abf1p and a T-rich promoter element in nucleosome organization of the yeast RPS28A gene"

Article Title: Different roles for Abf1p and a T-rich promoter element in nucleosome organization of the yeast RPS28A gene

Journal: Nucleic Acids Research

doi:

Comparison of in vivo nucleosome positions on the wild-type RPS28A promoter and a promoter containing a mutated T-rich element. Nystatin-permeabilized spheroplasts prepared from wild-type (ERS3) and mutant cells (ETS6) were treated with either Mnase ( A ) or DNaseI ( B ). Distances are from the translational start codon ATG. Protected regions of 160–170 bp are interpreted as positioned nucleosomes (closed elipses). The 130 bp Mnase-protected region that covers the Abf1p site ( Xba I) in the wild-type promoter (ERS3) has decreased to 85 bp when the T-rich element is mutated (ERS6). Concomitantly, downstream nucleosomes are shifted towards the Abf1p binding site.
Figure Legend Snippet: Comparison of in vivo nucleosome positions on the wild-type RPS28A promoter and a promoter containing a mutated T-rich element. Nystatin-permeabilized spheroplasts prepared from wild-type (ERS3) and mutant cells (ETS6) were treated with either Mnase ( A ) or DNaseI ( B ). Distances are from the translational start codon ATG. Protected regions of 160–170 bp are interpreted as positioned nucleosomes (closed elipses). The 130 bp Mnase-protected region that covers the Abf1p site ( Xba I) in the wild-type promoter (ERS3) has decreased to 85 bp when the T-rich element is mutated (ERS6). Concomitantly, downstream nucleosomes are shifted towards the Abf1p binding site.

Techniques Used: In Vivo, Mutagenesis, Binding Assay

2) Product Images from "Temperature, template topology, and factor requirements of archaeal transcription"

Article Title: Temperature, template topology, and factor requirements of archaeal transcription

Journal: Proceedings of the National Academy of Sciences of the United States of America

doi:

DNaseI footprinting of archaeal TBP and TFB on the template strand of negatively supercoiled (σ = −0.053) 16S (lanes 1–7) and positively supercoiled (σ = +0.047) 16S templates (lanes 8–13). Lane 1 contains a ddA sequencing ladder. Reactions contain 0 pmol (lanes 2 and 8), 3 pmol (lanes 3 and 9), 1.5 pmol (lanes 4 and 10), 0.75 pmol (lanes 5 and 11), 0.38 pmol (lanes 6 and 12), and 0.19 pmol (lanes 7 and 13) of an equimolar mix of TBP and TFB. The region protected from cleavage by DNaseI is indicated by an open box; the TATA and initiator elements are shown as filled boxes.
Figure Legend Snippet: DNaseI footprinting of archaeal TBP and TFB on the template strand of negatively supercoiled (σ = −0.053) 16S (lanes 1–7) and positively supercoiled (σ = +0.047) 16S templates (lanes 8–13). Lane 1 contains a ddA sequencing ladder. Reactions contain 0 pmol (lanes 2 and 8), 3 pmol (lanes 3 and 9), 1.5 pmol (lanes 4 and 10), 0.75 pmol (lanes 5 and 11), 0.38 pmol (lanes 6 and 12), and 0.19 pmol (lanes 7 and 13) of an equimolar mix of TBP and TFB. The region protected from cleavage by DNaseI is indicated by an open box; the TATA and initiator elements are shown as filled boxes.

Techniques Used: Footprinting, Sequencing

Related Articles

Footprinting:

Article Title: Temperature, template topology, and factor requirements of archaeal transcription
Article Snippet: Reactions were incubated for 10 min at 48°C before the addition of DNaseI or KMnO4 . .. DNaseI footprinting was performed by adding 10 milliunits of DNaseI (Boehringer Mannheim) for 30 sec, followed by the addition of EDTA to 50 mM and SDS to 0.5%. .. DNA was recovered by phenol/chloroform extraction followed by salt exchange on a G50 gel filtration column.

Size-exclusion Chromatography:

Article Title: Temperature, template topology, and factor requirements of archaeal transcription
Article Snippet: Reactions were incubated for 10 min at 48°C before the addition of DNaseI or KMnO4 . .. DNaseI footprinting was performed by adding 10 milliunits of DNaseI (Boehringer Mannheim) for 30 sec, followed by the addition of EDTA to 50 mM and SDS to 0.5%. .. DNA was recovered by phenol/chloroform extraction followed by salt exchange on a G50 gel filtration column.

Incubation:

Article Title: Murid Herpesvirus-4 Exploits Dendritic Cells to Infect B Cells
Article Snippet: The medium was changed every 2 d, and non-adherent cells harvested after 7 d. These were > 90% GR-1- CD11c+ by flow cytometry. .. Bead-based cell separation Lymph nodes were removed post-mortem, finely minced, digested (20 min) with type II collagenase (1 mg/ml, Worthington Biochemicals) plus DNaseI (20 µg/ml, Boehringer Mannheim), then incubated (5 min) in 100 mM EDTA to disrupt cell/cell conjugates. ..

Article Title: Expression of the Voltage-Gated Chloride Channel ClC-2 in Rod Bipolar Cells of the Rat Retina
Article Snippet: cDNA synthesis and PCR. .. Five micrograms of rat retina poly(A+ ) RNA (Clontech, Palo Alto, CA) were incubated for 30 min at 37°C with 50 U of DNaseI (Boehringer Mannheim, Mannheim, Germany) and 40 U of RNasin (Boehringer Mannheim) in a final volume of 100 μl to remove possible contamination from chromosomal DNA. .. To remove the enzyme, acid phenol extraction and ethanol precipitation was performed by adding 16 μl of 2 m sodium acetate, pH 4.0, 100 μl of acid phenol, and 50 μl of chloroform/isoamylalcohol (49:1) following the protocol of .

Ethanol Precipitation:

Article Title: Random Monoallelic Expression of Three Genes Clustered within 60 kb of Mouse t Complex Genomic DNA
Article Snippet: After an additional 14 d of culture, cells were trypsinized, washed three times with PBS, and lysed in 5 mL of Trizol Reagent (GIBCO BRL) to isolate RNAs. .. To remove potential contamination of genomic DNA, we digested total RNAs with 1 unit of DNaseI (Boehringer Mannheim) for 15 min at 37°C, followed by phenol/chloroform extraction, and ethanol precipitation. .. RT-PCR and SNuPE assays were performed as described earlier.

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    Boehringer Mannheim dnasei
    <t>DNaseI</t> <t>footprinting</t> of archaeal TBP and TFB on the template strand of negatively supercoiled (σ = −0.053) 16S (lanes 1–7) and positively supercoiled (σ = +0.047) 16S templates (lanes 8–13). Lane 1 contains a ddA sequencing ladder. Reactions contain 0 pmol (lanes 2 and 8), 3 pmol (lanes 3 and 9), 1.5 pmol (lanes 4 and 10), 0.75 pmol (lanes 5 and 11), 0.38 pmol (lanes 6 and 12), and 0.19 pmol (lanes 7 and 13) of an equimolar mix of TBP and TFB. The region protected from cleavage by DNaseI is indicated by an open box; the TATA and initiator elements are shown as filled boxes.
    Dnasei, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnasei/product/Boehringer Mannheim
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dnasei - by Bioz Stars, 2021-04
    86/100 stars
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    DNaseI footprinting of archaeal TBP and TFB on the template strand of negatively supercoiled (σ = −0.053) 16S (lanes 1–7) and positively supercoiled (σ = +0.047) 16S templates (lanes 8–13). Lane 1 contains a ddA sequencing ladder. Reactions contain 0 pmol (lanes 2 and 8), 3 pmol (lanes 3 and 9), 1.5 pmol (lanes 4 and 10), 0.75 pmol (lanes 5 and 11), 0.38 pmol (lanes 6 and 12), and 0.19 pmol (lanes 7 and 13) of an equimolar mix of TBP and TFB. The region protected from cleavage by DNaseI is indicated by an open box; the TATA and initiator elements are shown as filled boxes.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Temperature, template topology, and factor requirements of archaeal transcription

    doi:

    Figure Lengend Snippet: DNaseI footprinting of archaeal TBP and TFB on the template strand of negatively supercoiled (σ = −0.053) 16S (lanes 1–7) and positively supercoiled (σ = +0.047) 16S templates (lanes 8–13). Lane 1 contains a ddA sequencing ladder. Reactions contain 0 pmol (lanes 2 and 8), 3 pmol (lanes 3 and 9), 1.5 pmol (lanes 4 and 10), 0.75 pmol (lanes 5 and 11), 0.38 pmol (lanes 6 and 12), and 0.19 pmol (lanes 7 and 13) of an equimolar mix of TBP and TFB. The region protected from cleavage by DNaseI is indicated by an open box; the TATA and initiator elements are shown as filled boxes.

    Article Snippet: DNaseI footprinting was performed by adding 10 milliunits of DNaseI (Boehringer Mannheim) for 30 sec, followed by the addition of EDTA to 50 mM and SDS to 0.5%.

    Techniques: Footprinting, Sequencing

    Comparison of in vivo nucleosome positions on the wild-type RPS28A promoter and a promoter containing a mutated T-rich element. Nystatin-permeabilized spheroplasts prepared from wild-type (ERS3) and mutant cells (ETS6) were treated with either Mnase ( A ) or DNaseI ( B ). Distances are from the translational start codon ATG. Protected regions of 160–170 bp are interpreted as positioned nucleosomes (closed elipses). The 130 bp Mnase-protected region that covers the Abf1p site ( Xba I) in the wild-type promoter (ERS3) has decreased to 85 bp when the T-rich element is mutated (ERS6). Concomitantly, downstream nucleosomes are shifted towards the Abf1p binding site.

    Journal: Nucleic Acids Research

    Article Title: Different roles for Abf1p and a T-rich promoter element in nucleosome organization of the yeast RPS28A gene

    doi:

    Figure Lengend Snippet: Comparison of in vivo nucleosome positions on the wild-type RPS28A promoter and a promoter containing a mutated T-rich element. Nystatin-permeabilized spheroplasts prepared from wild-type (ERS3) and mutant cells (ETS6) were treated with either Mnase ( A ) or DNaseI ( B ). Distances are from the translational start codon ATG. Protected regions of 160–170 bp are interpreted as positioned nucleosomes (closed elipses). The 130 bp Mnase-protected region that covers the Abf1p site ( Xba I) in the wild-type promoter (ERS3) has decreased to 85 bp when the T-rich element is mutated (ERS6). Concomitantly, downstream nucleosomes are shifted towards the Abf1p binding site.

    Article Snippet: For chromatin samples, after the 0 min time sample, either 250 U/4 ml Mnase (micrococcal endonuclease) or 180 U/4 ml DNaseI (both from Boehringer-Mannheim) was added to the nystatin-permeabilized spheroplasts, and 500 µl time samples (1, 2.5, 5, 9, 15 and 25 min) were added to 50 µl StopMnase (10% SDS, 0.1 M EDTA and 50 µg proteinase K).

    Techniques: In Vivo, Mutagenesis, Binding Assay