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Applichem dnasei
Dnasei, supplied by Applichem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dnasei/product/Applichem
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
dnasei - by Bioz Stars, 2021-04
86/100 stars

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Flow Cytometry:

Article Title: Topological Small-World Organization of the Fibroblastic Reticular Cell Network Determines Lymph Node Functionality
Article Snippet: .. LN Cellularity For flow cytometric analysis of LN cellularity, inguinal LNs from individual mice were pooled and digested on 37°C in RPMI containing 2% FCS, 20 mM Hepes (all from Lonza), 1 mg/ml Collagenase Type P (Sigma-Aldrich), and 25 μg/ml DNaseI (AppliChem) for 20 min. After enzymatic digestion, cell suspensions were washed with PBS and stained using the following antibodies: CD3-PE (BD Bioscience), CD8-PeCy7, CD4-FITC, CD45-APC-H7, MHCII-PE, CD11c-PeCy7, B220-APC (BioLegend). .. In flow cytometric analyses, 7-amino-actinomycin D (7AAD; Calbiochem) was used to discriminate dead cells.

Mouse Assay:

Article Title: Topological Small-World Organization of the Fibroblastic Reticular Cell Network Determines Lymph Node Functionality
Article Snippet: .. LN Cellularity For flow cytometric analysis of LN cellularity, inguinal LNs from individual mice were pooled and digested on 37°C in RPMI containing 2% FCS, 20 mM Hepes (all from Lonza), 1 mg/ml Collagenase Type P (Sigma-Aldrich), and 25 μg/ml DNaseI (AppliChem) for 20 min. After enzymatic digestion, cell suspensions were washed with PBS and stained using the following antibodies: CD3-PE (BD Bioscience), CD8-PeCy7, CD4-FITC, CD45-APC-H7, MHCII-PE, CD11c-PeCy7, B220-APC (BioLegend). .. In flow cytometric analyses, 7-amino-actinomycin D (7AAD; Calbiochem) was used to discriminate dead cells.

Article Title: Human umbilical cord tissue-derived mesenchymal stromal cells attenuate remodeling after myocardial infarction by proangiogenic, antiapoptotic, and endogenous cell-activation mechanisms
Article Snippet: Isolation and culture of adult murine heart Sca-1+ cardiac progenitor cells (CPCs) Sca-1+ CPCs were extracted and selected by immunomagnetic cell sorting (MACS; Miltenyi Biotec, Bergisch Gladbach, Germany). .. In brief, hearts from six adult C57BL/6 mice were harvested, dissociated by using the GentleMACS Dissociator (Miltenyi Biotec), and digested with 600 U/ml Collagenase II (4176; Worthington, Lakewood, NJ, USA) and 60 U/ml DNAseI (A3778; Applichem Lifesciences, Gatersleben, Germany) for 30 minutes at 37°C with agitation (100 rpm). .. Immunomagnetic cell sorting was performed by incubating the single-cell suspension with Sca-1-FITC antibody (MACS; Miltenyi Biotec) followed by anti-FITC Microbeads (MACS; Miltenyi Biotec).

Staining:

Article Title: Topological Small-World Organization of the Fibroblastic Reticular Cell Network Determines Lymph Node Functionality
Article Snippet: .. LN Cellularity For flow cytometric analysis of LN cellularity, inguinal LNs from individual mice were pooled and digested on 37°C in RPMI containing 2% FCS, 20 mM Hepes (all from Lonza), 1 mg/ml Collagenase Type P (Sigma-Aldrich), and 25 μg/ml DNaseI (AppliChem) for 20 min. After enzymatic digestion, cell suspensions were washed with PBS and stained using the following antibodies: CD3-PE (BD Bioscience), CD8-PeCy7, CD4-FITC, CD45-APC-H7, MHCII-PE, CD11c-PeCy7, B220-APC (BioLegend). .. In flow cytometric analyses, 7-amino-actinomycin D (7AAD; Calbiochem) was used to discriminate dead cells.

Isolation:

Article Title: Central Nervous System Stromal Cells Control Local CD8+ T Cell Responses during Virus-Induced Neuroinflammation
Article Snippet: Cell Isolation from Brain Tissues Mice were sacrificed at the indicated time points and immediately perfused with PBS. .. CNS-infiltrating lymphocytes were isolated using mechanical disruption of the organ followed by enrichment based on 70%–30% Percoll gradients (GE Healthcare) and centrifugation for 25 min at 800 × g. For isolation of stromal cells from olfactory bulbs, the tissue was cut into small pieces, transferred into a 24-well dish filled with RPMI 1640 medium containing 2% FCS, 20 mM HEPES (all from Lonza), 1 mg/ml Collagenase Type P (Sigma-Aldrich), and 25 μg/ml DNaseI (AppliChem), and incubated at 37°C for 30 min. After enzymatic digestion, cell suspensions were washed with PBS containing 0.5% FCS and 10 mM EDTA (MACS buffer). .. To enrich the stromal cell fraction, we removed myelin-producing cells using 30% Percoll and 15 min centrifugation at 700 × g followed by hematopoietic cell depletion using MACS anti-CD45 and anti-Ter119 Microbeads (Miltenyi).

Article Title: Central Nervous System Stromal Cells Control Local CD8(+) T Cell Responses during Virus-Induced Neuroinflammation.
Article Snippet: Cell Isolation from Brain Tissues Mice were sacrificed at the indicated time points and immediately perfused with PBS. .. CNS-infiltrating lymphocytes were isolated using mechanical disruption of the organ followed by enrichment based on 70%-30% Percoll gradients (GE Healthcare) and centrifugation for 25 min at 800 3 g. For isolation of stromal cells from olfactory bulbs, the tissue was cut into small pieces, transferred into a 24-well dish filled with RPMI 1640 medium containing 2% FCS, 20 mM HEPES (all from Lonza), 1 mg/ml Collagenase Type P (Sigma-Aldrich), and 25 mg/ml DNaseI (AppliChem), and incubated at 37 C for 30 min. After enzymatic digestion, cell suspensions were washed with PBS containing 0.5% FCS and 10 mM EDTA (MACS buffer). .. To enrich the stromal cell fraction, we removed myelin-producing cells using 30% Percoll and 15 min centrifugation at 700 3 g followed by hematopoietic cell depletion using MACS anti-CD45 and anti-Ter119 Microbeads (Miltenyi).

Centrifugation:

Article Title: Central Nervous System Stromal Cells Control Local CD8+ T Cell Responses during Virus-Induced Neuroinflammation
Article Snippet: Cell Isolation from Brain Tissues Mice were sacrificed at the indicated time points and immediately perfused with PBS. .. CNS-infiltrating lymphocytes were isolated using mechanical disruption of the organ followed by enrichment based on 70%–30% Percoll gradients (GE Healthcare) and centrifugation for 25 min at 800 × g. For isolation of stromal cells from olfactory bulbs, the tissue was cut into small pieces, transferred into a 24-well dish filled with RPMI 1640 medium containing 2% FCS, 20 mM HEPES (all from Lonza), 1 mg/ml Collagenase Type P (Sigma-Aldrich), and 25 μg/ml DNaseI (AppliChem), and incubated at 37°C for 30 min. After enzymatic digestion, cell suspensions were washed with PBS containing 0.5% FCS and 10 mM EDTA (MACS buffer). .. To enrich the stromal cell fraction, we removed myelin-producing cells using 30% Percoll and 15 min centrifugation at 700 × g followed by hematopoietic cell depletion using MACS anti-CD45 and anti-Ter119 Microbeads (Miltenyi).

Article Title: Central Nervous System Stromal Cells Control Local CD8(+) T Cell Responses during Virus-Induced Neuroinflammation.
Article Snippet: Cell Isolation from Brain Tissues Mice were sacrificed at the indicated time points and immediately perfused with PBS. .. CNS-infiltrating lymphocytes were isolated using mechanical disruption of the organ followed by enrichment based on 70%-30% Percoll gradients (GE Healthcare) and centrifugation for 25 min at 800 3 g. For isolation of stromal cells from olfactory bulbs, the tissue was cut into small pieces, transferred into a 24-well dish filled with RPMI 1640 medium containing 2% FCS, 20 mM HEPES (all from Lonza), 1 mg/ml Collagenase Type P (Sigma-Aldrich), and 25 mg/ml DNaseI (AppliChem), and incubated at 37 C for 30 min. After enzymatic digestion, cell suspensions were washed with PBS containing 0.5% FCS and 10 mM EDTA (MACS buffer). .. To enrich the stromal cell fraction, we removed myelin-producing cells using 30% Percoll and 15 min centrifugation at 700 3 g followed by hematopoietic cell depletion using MACS anti-CD45 and anti-Ter119 Microbeads (Miltenyi).

Incubation:

Article Title: Central Nervous System Stromal Cells Control Local CD8+ T Cell Responses during Virus-Induced Neuroinflammation
Article Snippet: Cell Isolation from Brain Tissues Mice were sacrificed at the indicated time points and immediately perfused with PBS. .. CNS-infiltrating lymphocytes were isolated using mechanical disruption of the organ followed by enrichment based on 70%–30% Percoll gradients (GE Healthcare) and centrifugation for 25 min at 800 × g. For isolation of stromal cells from olfactory bulbs, the tissue was cut into small pieces, transferred into a 24-well dish filled with RPMI 1640 medium containing 2% FCS, 20 mM HEPES (all from Lonza), 1 mg/ml Collagenase Type P (Sigma-Aldrich), and 25 μg/ml DNaseI (AppliChem), and incubated at 37°C for 30 min. After enzymatic digestion, cell suspensions were washed with PBS containing 0.5% FCS and 10 mM EDTA (MACS buffer). .. To enrich the stromal cell fraction, we removed myelin-producing cells using 30% Percoll and 15 min centrifugation at 700 × g followed by hematopoietic cell depletion using MACS anti-CD45 and anti-Ter119 Microbeads (Miltenyi).

Article Title: Central Nervous System Stromal Cells Control Local CD8(+) T Cell Responses during Virus-Induced Neuroinflammation.
Article Snippet: Cell Isolation from Brain Tissues Mice were sacrificed at the indicated time points and immediately perfused with PBS. .. CNS-infiltrating lymphocytes were isolated using mechanical disruption of the organ followed by enrichment based on 70%-30% Percoll gradients (GE Healthcare) and centrifugation for 25 min at 800 3 g. For isolation of stromal cells from olfactory bulbs, the tissue was cut into small pieces, transferred into a 24-well dish filled with RPMI 1640 medium containing 2% FCS, 20 mM HEPES (all from Lonza), 1 mg/ml Collagenase Type P (Sigma-Aldrich), and 25 mg/ml DNaseI (AppliChem), and incubated at 37 C for 30 min. After enzymatic digestion, cell suspensions were washed with PBS containing 0.5% FCS and 10 mM EDTA (MACS buffer). .. To enrich the stromal cell fraction, we removed myelin-producing cells using 30% Percoll and 15 min centrifugation at 700 3 g followed by hematopoietic cell depletion using MACS anti-CD45 and anti-Ter119 Microbeads (Miltenyi).

Magnetic Cell Separation:

Article Title: Central Nervous System Stromal Cells Control Local CD8+ T Cell Responses during Virus-Induced Neuroinflammation
Article Snippet: Cell Isolation from Brain Tissues Mice were sacrificed at the indicated time points and immediately perfused with PBS. .. CNS-infiltrating lymphocytes were isolated using mechanical disruption of the organ followed by enrichment based on 70%–30% Percoll gradients (GE Healthcare) and centrifugation for 25 min at 800 × g. For isolation of stromal cells from olfactory bulbs, the tissue was cut into small pieces, transferred into a 24-well dish filled with RPMI 1640 medium containing 2% FCS, 20 mM HEPES (all from Lonza), 1 mg/ml Collagenase Type P (Sigma-Aldrich), and 25 μg/ml DNaseI (AppliChem), and incubated at 37°C for 30 min. After enzymatic digestion, cell suspensions were washed with PBS containing 0.5% FCS and 10 mM EDTA (MACS buffer). .. To enrich the stromal cell fraction, we removed myelin-producing cells using 30% Percoll and 15 min centrifugation at 700 × g followed by hematopoietic cell depletion using MACS anti-CD45 and anti-Ter119 Microbeads (Miltenyi).

Article Title: Central Nervous System Stromal Cells Control Local CD8(+) T Cell Responses during Virus-Induced Neuroinflammation.
Article Snippet: Cell Isolation from Brain Tissues Mice were sacrificed at the indicated time points and immediately perfused with PBS. .. CNS-infiltrating lymphocytes were isolated using mechanical disruption of the organ followed by enrichment based on 70%-30% Percoll gradients (GE Healthcare) and centrifugation for 25 min at 800 3 g. For isolation of stromal cells from olfactory bulbs, the tissue was cut into small pieces, transferred into a 24-well dish filled with RPMI 1640 medium containing 2% FCS, 20 mM HEPES (all from Lonza), 1 mg/ml Collagenase Type P (Sigma-Aldrich), and 25 mg/ml DNaseI (AppliChem), and incubated at 37 C for 30 min. After enzymatic digestion, cell suspensions were washed with PBS containing 0.5% FCS and 10 mM EDTA (MACS buffer). .. To enrich the stromal cell fraction, we removed myelin-producing cells using 30% Percoll and 15 min centrifugation at 700 3 g followed by hematopoietic cell depletion using MACS anti-CD45 and anti-Ter119 Microbeads (Miltenyi).

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    Applichem dnasei
    Dnasei, supplied by Applichem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnasei/product/Applichem
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dnasei - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    PanReac AppliChem dnasei
    Dnasei, supplied by PanReac AppliChem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnasei/product/PanReac AppliChem
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dnasei - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

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