dnase (New England Biolabs)


Structured Review

Dnase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dnase/product/New England Biolabs
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Holliday junction affinity of the base excision repair factor Endo III contributes to cholera toxin phage integration"
Article Title: Holliday junction affinity of the base excision repair factor Endo III contributes to cholera toxin phage integration
Journal: The EMBO Journal
doi: 10.1038/emboj.2012.219

Figure Legend Snippet: Endo III blocks XerC-catalysis on pseudo-HJ and displaces it from them. ( A ) Scheme of the suicide pseudo-HJ indicating the mismatch engineered to slow down re-ligation after XerC-cleavage. KMnO 4 sensitive residues in the XerC- and XerD-binding att P(+) arms are indicated by a star. ( B ) Resolution of att P(+)/ dif suicide pseudo-HJs. Legend as in Figure 4D . ( C ) DNase I protection and ( D ) KMnO 4 sensitivity assays of the att P(+)/ dif 1 pseudo-HJ substrate. The analysed strand was labelled on its 5′ end. A scheme of the analysed strand is drawn on the left of the gels. KMnO 4 sensitive residues in the XerC- and XerD-binding sites are indicated by a star.
Techniques Used: Ligation, Binding Assay
2) Product Images from "Comparison of Blood RNA Extraction Methods Used for Gene Expression Profiling in Amyotrophic Lateral Sclerosis"
Article Title: Comparison of Blood RNA Extraction Methods Used for Gene Expression Profiling in Amyotrophic Lateral Sclerosis
Journal: PLoS ONE
doi: 10.1371/journal.pone.0087508

Figure Legend Snippet: Representative Bioanalyzer electropherograms after initial RNA extractions. High quality RNA (RIN > 7.0) can be extracted from all three methods. PAXGENE however requires a DNase step as traces from initial extractions show genomic DNA contamination at high molecular weights and as a “shoulder” to the 28S peak (indicated by asterisks). Good quality RNA can be detected after globin depletion with both TEM and PAXGENE. However, PAXGENE samples showed consistently lower concentration levels, as indicated by the smaller 18S and 28S peaks (FU, fluorescent units). Traces are representative and from single samples from each extraction method.
Techniques Used: Transmission Electron Microscopy, Concentration Assay
3) Product Images from "3′ IsomiR Species and DNA Contamination Influence Reliable Quantification of MicroRNAs by Stem-Loop Quantitative PCR"
Article Title: 3′ IsomiR Species and DNA Contamination Influence Reliable Quantification of MicroRNAs by Stem-Loop Quantitative PCR
Journal: PLoS ONE
doi: 10.1371/journal.pone.0106315

Figure Legend Snippet: DNA can serve as a template during miRNA detection. ( A ) False positive signal of DNA derives mainly from the reverse transcription reaction. Mature miR-1226-3p was tested in the indicated samples, with or without reverse transcription (RT). On the y-axis, dC t value is represented, calculated as the Ct difference between the examined samples and the gDNA of control HeLa_mir-33b cell line (Ct = 35,9). One C t difference represents about 2× higher detected mature miRNA level. ( B ) DNA contamination remains in total RNA samples during isolation by the widely used Trizol reagent. Total RNA samples were isolated from transiently transfected HeLa cells; the transfected plasmid DNA amounts are indicated. Samples were DNase treated and non-treated, then reverse transcribed and subjected to real-time PCR. Expression values relative to U6 snRNA are shown on the y-axis. Experiments were carried out with three replicates at least from three independent experiments; one representative experiment is shown, error bars represent standard deviations.
Techniques Used: Isolation, Transfection, Plasmid Preparation, Real-time Polymerase Chain Reaction, Expressing