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    Thermo Fisher dnase rnase free water
    Dnase Rnase Free Water, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 5 article reviews
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    dnase rnase free water - by Bioz Stars, 2020-04
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    Amplification:

    Article Title: Impact of DNA extraction method and targeted 16S-rRNA hypervariable region on oral microbiota profiling
    Article Snippet: Each qPCR was performed in a reaction volume of 20 μl containing 0.5 μM of each primer (Bac-16s-F2: 5′-TTAAACTCAAAGGAATTGACGG, Bac-16s-R2: 5′-CTCACGRCACGAGCTGACGAC) , 1 μl of genomic DNA, 10 μl of 2 × SYBR Premix EXTaq mix (Takara) and 5 μl sterilized DNase-RNase-free water in MicroAmp fast optical 96-well reaction plates (Applied Biosystems, USA) with adhesive sealing. .. The qPCR parameters were 95 °C for 10 min, 45 cycles of three amplification steps including: 95 °C for 15 s, 60 °C for 20 s and 72 °C for 15 s, and final cooling at 25 °C for 1 min.

    Article Title: Treponema pallidum Major Sheath Protein Homologue Tpr K Is a Target of Opsonic Antibody and the Protective Immune Response
    Article Snippet: Total RNA was extracted from a known number of freshly harvested treponemes followed by treatment of the RNA sample with RNAse free DNAse A ( GIBCO BRL ). .. First-strand cDNA was made using Superscript II and random primers ( GIBCO BRL ), and PCR amplification was performed using primers specific for each tpr .

    Article Title: Hydrophobic Amino Acids in the Human Immunodeficiency Virus Type 1 p2 and Nucleocapsid Proteins Can Contribute to the Rescue of Deleted Viral RNA Packaging Signals
    Article Snippet: Viral RNA was dissolved in 50 μl of diethyl pyrocarbonate-treated water and treated with 20 U of RNase-free DNase (Gibco-BRL, Montreal, Quebec, Canada) at 37°C for 30 min to remove any DNA contamination. .. Five-microliter volumes of viral RNA were amplified for 20 cycles with a Titan One-Tube RT-PCR system (Boehringer Mannheim, Mannheim, Germany) using primer pair pGAG1-pST to analyze full-length viral RNA ( ).

    Cytometry:

    Article Title: Use of SYTO 13, a fluorescent dye binding nucleic acids, for the detection of microparticles in in vitro systems
    Article Snippet: This volume of the enriched and permeabilized MP suspension was treated with 100 U/ml of RNase-free DNase (Invitrogen Co., Carlsbad, CA) or 200 U/ml of DNase-free RNase (Sigma-Aldrich Co.) or both and incubated at 37°C for 90 min and then assessed for the presence of nucleic acid. .. The undigested, control MPs were analyzed using flow cytometry after staining with PI.

    Construct:

    Article Title: Hydrophobic Amino Acids in the Human Immunodeficiency Virus Type 1 p2 and Nucleocapsid Proteins Can Contribute to the Rescue of Deleted Viral RNA Packaging Signals
    Article Snippet: Supernatants from COS-7 cells that had been transfected with various HIV-1 recombinant DNA constructs were clarified in a Beckman GS-6R centrifuge at 3,000 rpm for 30 min at 4°C and quantified on the basis of CA Ag levels. .. Viral RNA was dissolved in 50 μl of diethyl pyrocarbonate-treated water and treated with 20 U of RNase-free DNase (Gibco-BRL, Montreal, Quebec, Canada) at 37°C for 30 min to remove any DNA contamination.

    Real-time Polymerase Chain Reaction:

    Article Title: The Staphylococcus-Specific Gene rsr Represses agr and Virulence in Staphylococcus aureus ▿
    Article Snippet: Paragraph title: Real-time PCR analysis of gene transcription. ... RNA, prepared as described above, was treated with RNase-free DNase to remove residual DNA, according to the manufacturer's instructions (Turbo-DNAfree; Applied Biosystems/Ambion, Austin, TX).

    Article Title: Impact of DNA extraction method and targeted 16S-rRNA hypervariable region on oral microbiota profiling
    Article Snippet: .. Each qPCR was performed in a reaction volume of 20 μl containing 0.5 μM of each primer (Bac-16s-F2: 5′-TTAAACTCAAAGGAATTGACGG, Bac-16s-R2: 5′-CTCACGRCACGAGCTGACGAC) , 1 μl of genomic DNA, 10 μl of 2 × SYBR Premix EXTaq mix (Takara) and 5 μl sterilized DNase-RNase-free water in MicroAmp fast optical 96-well reaction plates (Applied Biosystems, USA) with adhesive sealing. .. The qPCR parameters were 95 °C for 10 min, 45 cycles of three amplification steps including: 95 °C for 15 s, 60 °C for 20 s and 72 °C for 15 s, and final cooling at 25 °C for 1 min.

    Incubation:

    Article Title: Use of SYTO 13, a fluorescent dye binding nucleic acids, for the detection of microparticles in in vitro systems
    Article Snippet: .. This volume of the enriched and permeabilized MP suspension was treated with 100 U/ml of RNase-free DNase (Invitrogen Co., Carlsbad, CA) or 200 U/ml of DNase-free RNase (Sigma-Aldrich Co.) or both and incubated at 37°C for 90 min and then assessed for the presence of nucleic acid. .. The final volume of the treated MP suspension was brought up to 1 ml by adding more BD Perm/Wash™ buffer.

    Article Title: Artificial MicroRNAs Targeting C9orf72 Can Reduce Accumulation of Intra-nuclear Transcripts in ALS and FTD Patients
    Article Snippet: Optionally, cells were treated for 30 min with 5 mg/mL RNase A (QIAGEN) or 100 U RNase-free DNase (Invitrogen). .. The cells were incubated for 1 h in hybridization buffer (50% formamide, 10% dextran sulfate, 0.1 mg/mL yeast tRNA,2×saline-sodium citrate (SSC), and 50 mM sodium phosphate) at 37°C and hybridized overnight with 40 nm TYE563-(C4 G2 )3 LNA probe in hybridization buffer at 37°C.

    Activity Assay:

    Article Title: Factors affecting the yield of microRNAs from laser microdissectates of formalin-fixed tissue sections
    Article Snippet: Nuclease treatment of RNA preparations Bovine pancreas RNAse A (DNAse- and proteinase-free) and recombinant DNAse I (RNAse-free) were obtained from Fermentas® (Glen Burnie, MD). .. Control reactions using yeast tRNA (0.1-0.2 μg) confirmed completeness of the RNAse reactions and absence of RNAse activity in the DNAse I stock.

    Expressing:

    Article Title: The Staphylococcus-Specific Gene rsr Represses agr and Virulence in Staphylococcus aureus ▿
    Article Snippet: RNA, prepared as described above, was treated with RNase-free DNase to remove residual DNA, according to the manufacturer's instructions (Turbo-DNAfree; Applied Biosystems/Ambion, Austin, TX). .. Reported levels of gene expression were normalized to those of gyrB .

    Hybridization:

    Article Title: Artificial MicroRNAs Targeting C9orf72 Can Reduce Accumulation of Intra-nuclear Transcripts in ALS and FTD Patients
    Article Snippet: Optionally, cells were treated for 30 min with 5 mg/mL RNase A (QIAGEN) or 100 U RNase-free DNase (Invitrogen). .. The cells were incubated for 1 h in hybridization buffer (50% formamide, 10% dextran sulfate, 0.1 mg/mL yeast tRNA,2×saline-sodium citrate (SSC), and 50 mM sodium phosphate) at 37°C and hybridized overnight with 40 nm TYE563-(C4 G2 )3 LNA probe in hybridization buffer at 37°C.

    Flow Cytometry:

    Article Title: Use of SYTO 13, a fluorescent dye binding nucleic acids, for the detection of microparticles in in vitro systems
    Article Snippet: This volume of the enriched and permeabilized MP suspension was treated with 100 U/ml of RNase-free DNase (Invitrogen Co., Carlsbad, CA) or 200 U/ml of DNase-free RNase (Sigma-Aldrich Co.) or both and incubated at 37°C for 90 min and then assessed for the presence of nucleic acid. .. The undigested, control MPs were analyzed using flow cytometry after staining with PI.

    Infection:

    Article Title: Packaging of viral RNAs in virions of adenoviruses
    Article Snippet: The isolated virion RNAs were treated with RNase-free DNase (Ambion) to eliminate the contaminated viral genomic DNA, followed by addition of 0.1 volume DNase inactivation reagent (DNA-free kit, Ambion). .. The RNA was also isolated from wt PAdV-3 infected cells as described previously [ ].

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: A 5'-proximal Stem-loop Structure of 5' Untranslated Region of Porcine Reproductive and Respiratory Syndrome Virus Genome Is Key for Virus Replication
    Article Snippet: Paragraph title: Detection of (-) gRNAs and sg mRNAs by RT-PCR ... RNAs were suspended in DNase/RNase-free water, and quantified by NanoDrop® ND-1000 (Thermo Fisher Scientific Inc.).

    Article Title: A 5'-proximal Stem-loop Structure of 5' Untranslated Region of Porcine Reproductive and Respiratory Syndrome Virus Genome Is Key for Virus Replication
    Article Snippet: .. To eliminate the transfected input DNA, the RNA preparation was further treated with 2 U RNase-free recombinant DNase I for 30 minutes at 37°C by using the DNA-free Kit (Ambion), followed by re-suspension in RNase-free H2 O. RT-PCR was employed to detect the (-) gRNAs and sg mRNAs using specific primers. ..

    Article Title: Treponema pallidum Major Sheath Protein Homologue Tpr K Is a Target of Opsonic Antibody and the Protective Immune Response
    Article Snippet: Reverse transcription (RT)-PCR systems were developed for semiquantitative and qualitative detection of mRNA for the 12 tpr genes of T . pallidum subspecies pallidum . .. Total RNA was extracted from a known number of freshly harvested treponemes followed by treatment of the RNA sample with RNAse free DNAse A ( GIBCO BRL ).

    Article Title: Hydrophobic Amino Acids in the Human Immunodeficiency Virus Type 1 p2 and Nucleocapsid Proteins Can Contribute to the Rescue of Deleted Viral RNA Packaging Signals
    Article Snippet: Paragraph title: Encapsidation of viral RNA by RT-PCR. ... Viral RNA was dissolved in 50 μl of diethyl pyrocarbonate-treated water and treated with 20 U of RNase-free DNase (Gibco-BRL, Montreal, Quebec, Canada) at 37°C for 30 min to remove any DNA contamination.

    Article Title: Release of targeted p53 from the mitochondrion as an early signal during mitochondrial dysfunction
    Article Snippet: Paragraph title: 2.6. Reverse transcription/polymerase chain reaction (RT-PCR) analysis ... After an 1 h RNase-free DNase digestion, 2μg of RNA was reversed transcribed with Superscript II Reverse transcriptase (Gibco-BRL, Gaithersburg, MD) at 42 °C in the presence of dithiothreitol (DTT), random primers, and 0.2 mM each dNTP.

    Recombinant:

    Article Title: A 5'-proximal Stem-loop Structure of 5' Untranslated Region of Porcine Reproductive and Respiratory Syndrome Virus Genome Is Key for Virus Replication
    Article Snippet: RNAs were suspended in DNase/RNase-free water, and quantified by NanoDrop® ND-1000 (Thermo Fisher Scientific Inc.). .. To eliminate the transfected input DNA, the RNA preparation was further treated with 2 U RNase-free recombinant DNase I for 30 minutes at 37°C by using the DNA-free Kit (Ambion), followed by re-suspension in RNase-free H2 O. RT-PCR was employed to detect the (-) gRNAs and sg mRNAs using specific primers.

    Article Title: A 5'-proximal Stem-loop Structure of 5' Untranslated Region of Porcine Reproductive and Respiratory Syndrome Virus Genome Is Key for Virus Replication
    Article Snippet: .. To eliminate the transfected input DNA, the RNA preparation was further treated with 2 U RNase-free recombinant DNase I for 30 minutes at 37°C by using the DNA-free Kit (Ambion), followed by re-suspension in RNase-free H2 O. RT-PCR was employed to detect the (-) gRNAs and sg mRNAs using specific primers. ..

    Article Title: Factors affecting the yield of microRNAs from laser microdissectates of formalin-fixed tissue sections
    Article Snippet: .. Nuclease treatment of RNA preparations Bovine pancreas RNAse A (DNAse- and proteinase-free) and recombinant DNAse I (RNAse-free) were obtained from Fermentas® (Glen Burnie, MD). .. Ten μl of nuclease reactions were set up at 37°C for 1 h using 1 U of either enzyme, buffer provided by Fermentas® for use with DNAse I, and 8 μl of RNA preparation containing < 0.1 μg RNA as per RiboGreen assay.

    Article Title: Hydrophobic Amino Acids in the Human Immunodeficiency Virus Type 1 p2 and Nucleocapsid Proteins Can Contribute to the Rescue of Deleted Viral RNA Packaging Signals
    Article Snippet: Supernatants from COS-7 cells that had been transfected with various HIV-1 recombinant DNA constructs were clarified in a Beckman GS-6R centrifuge at 3,000 rpm for 30 min at 4°C and quantified on the basis of CA Ag levels. .. Viral RNA was dissolved in 50 μl of diethyl pyrocarbonate-treated water and treated with 20 U of RNase-free DNase (Gibco-BRL, Montreal, Quebec, Canada) at 37°C for 30 min to remove any DNA contamination.

    Immunofluorescence:

    Article Title: A redox mechanism underlying nucleolar stress sensing by nucleophosmin
    Article Snippet: .. RNase A and DNase I digestion HeLa cells grown on coverslips were permeabilized with 0.1% Triton X-100 in PBS for 2 min, washed immediately and treated with RNase A (EN0531, 1 mg ml−1 , Fermentas, Thermo) or DNase I (EN0523, 0.5 U μl−1 , Fermentas, Thermo) in solution buffer for 10 min at 37 °C and cells were then fixed with 4% paraformaldehyde for 10 min and analysed by immunofluorescence. .. RNA immunoprecipitation HEK293T cells were scraped into 1 ml PBS.

    Fluorescence:

    Article Title: Impact of DNA extraction method and targeted 16S-rRNA hypervariable region on oral microbiota profiling
    Article Snippet: Each qPCR was performed in a reaction volume of 20 μl containing 0.5 μM of each primer (Bac-16s-F2: 5′-TTAAACTCAAAGGAATTGACGG, Bac-16s-R2: 5′-CTCACGRCACGAGCTGACGAC) , 1 μl of genomic DNA, 10 μl of 2 × SYBR Premix EXTaq mix (Takara) and 5 μl sterilized DNase-RNase-free water in MicroAmp fast optical 96-well reaction plates (Applied Biosystems, USA) with adhesive sealing. .. Reaction specificities were confirmed by melting curve analysis with a progressive increase in temperature from 65 to 94 °C at a 1 °C/sec transition rate and continuous fluorescence acquisition.

    Article Title: Artificial MicroRNAs Targeting C9orf72 Can Reduce Accumulation of Intra-nuclear Transcripts in ALS and FTD Patients
    Article Snippet: Optionally, cells were treated for 30 min with 5 mg/mL RNase A (QIAGEN) or 100 U RNase-free DNase (Invitrogen). .. The slides were mounted with ProLong Gold Antifade Mountant with DAPI (Invitrogen) and visualized using a LEICA DM2500 fluorescence microscope.

    Mutagenesis:

    Article Title: A 5'-proximal Stem-loop Structure of 5' Untranslated Region of Porcine Reproductive and Respiratory Syndrome Virus Genome Is Key for Virus Replication
    Article Snippet: Detection of (-) gRNAs and sg mRNAs by RT-PCR BHK-21 cells in six-well plates were transfected by the mutant plasmids as described above. .. RNAs were suspended in DNase/RNase-free water, and quantified by NanoDrop® ND-1000 (Thermo Fisher Scientific Inc.).

    Isolation:

    Article Title: Use of SYTO 13, a fluorescent dye binding nucleic acids, for the detection of microparticles in in vitro systems
    Article Snippet: Briefly, the MP pellet isolated from 2.5 × 106 Jurkat cells was resuspended in 250 μl of BD Cytofix/Cytoperm™ solution in a microfuge tube and incubated at 4°C for 20 min. MPs were then pelleted at 16,000×g for 30 min and washed twice in 500 μl of the BD Perm/Wash™ buffer containing a permeabilizing agent (saponin) and resuspended in 200 μl of the same buffer. .. This volume of the enriched and permeabilized MP suspension was treated with 100 U/ml of RNase-free DNase (Invitrogen Co., Carlsbad, CA) or 200 U/ml of DNase-free RNase (Sigma-Aldrich Co.) or both and incubated at 37°C for 90 min and then assessed for the presence of nucleic acid.

    Article Title: A 5'-proximal Stem-loop Structure of 5' Untranslated Region of Porcine Reproductive and Respiratory Syndrome Virus Genome Is Key for Virus Replication
    Article Snippet: Total cellular RNAs were isolated from the tranfected cells at 24 hpt using TRIzol® Reagent (Invitrogen). .. RNAs were suspended in DNase/RNase-free water, and quantified by NanoDrop® ND-1000 (Thermo Fisher Scientific Inc.).

    Article Title: Release of targeted p53 from the mitochondrion as an early signal during mitochondrial dysfunction
    Article Snippet: Total RNA was isolated from p53 wild-type and p53-null cells using RNeasy (Qiagen, Valencia, CA) for four biological replicates. .. After an 1 h RNase-free DNase digestion, 2μg of RNA was reversed transcribed with Superscript II Reverse transcriptase (Gibco-BRL, Gaithersburg, MD) at 42 °C in the presence of dithiothreitol (DTT), random primers, and 0.2 mM each dNTP.

    Article Title: Packaging of viral RNAs in virions of adenoviruses
    Article Snippet: .. The isolated virion RNAs were treated with RNase-free DNase (Ambion) to eliminate the contaminated viral genomic DNA, followed by addition of 0.1 volume DNase inactivation reagent (DNA-free kit, Ambion). .. The RNA was also isolated from wt PAdV-3 infected cells as described previously [ ].

    Transfection:

    Article Title: A 5'-proximal Stem-loop Structure of 5' Untranslated Region of Porcine Reproductive and Respiratory Syndrome Virus Genome Is Key for Virus Replication
    Article Snippet: Detection of (-) gRNAs and sg mRNAs by RT-PCR BHK-21 cells in six-well plates were transfected by the mutant plasmids as described above. .. RNAs were suspended in DNase/RNase-free water, and quantified by NanoDrop® ND-1000 (Thermo Fisher Scientific Inc.).

    Article Title: A 5'-proximal Stem-loop Structure of 5' Untranslated Region of Porcine Reproductive and Respiratory Syndrome Virus Genome Is Key for Virus Replication
    Article Snippet: .. To eliminate the transfected input DNA, the RNA preparation was further treated with 2 U RNase-free recombinant DNase I for 30 minutes at 37°C by using the DNA-free Kit (Ambion), followed by re-suspension in RNase-free H2 O. RT-PCR was employed to detect the (-) gRNAs and sg mRNAs using specific primers. ..

    Article Title: Hydrophobic Amino Acids in the Human Immunodeficiency Virus Type 1 p2 and Nucleocapsid Proteins Can Contribute to the Rescue of Deleted Viral RNA Packaging Signals
    Article Snippet: Supernatants from COS-7 cells that had been transfected with various HIV-1 recombinant DNA constructs were clarified in a Beckman GS-6R centrifuge at 3,000 rpm for 30 min at 4°C and quantified on the basis of CA Ag levels. .. Viral RNA was dissolved in 50 μl of diethyl pyrocarbonate-treated water and treated with 20 U of RNase-free DNase (Gibco-BRL, Montreal, Quebec, Canada) at 37°C for 30 min to remove any DNA contamination.

    Article Title: Artificial MicroRNAs Targeting C9orf72 Can Reduce Accumulation of Intra-nuclear Transcripts in ALS and FTD Patients
    Article Snippet: The cells were transfected on day 2 with 200 ng (G4 C2 )44 or (G4 C2 )3 plasmid. .. Optionally, cells were treated for 30 min with 5 mg/mL RNase A (QIAGEN) or 100 U RNase-free DNase (Invitrogen).

    Microscopy:

    Article Title: Artificial MicroRNAs Targeting C9orf72 Can Reduce Accumulation of Intra-nuclear Transcripts in ALS and FTD Patients
    Article Snippet: Optionally, cells were treated for 30 min with 5 mg/mL RNase A (QIAGEN) or 100 U RNase-free DNase (Invitrogen). .. The slides were mounted with ProLong Gold Antifade Mountant with DAPI (Invitrogen) and visualized using a LEICA DM2500 fluorescence microscope.

    Purification:

    Article Title: Hydrophobic Amino Acids in the Human Immunodeficiency Virus Type 1 p2 and Nucleocapsid Proteins Can Contribute to the Rescue of Deleted Viral RNA Packaging Signals
    Article Snippet: Viral RNA was purified from an amount of virus containing 2 ng of CA Ag using an RNA extraction kit (Qiagen). .. Viral RNA was dissolved in 50 μl of diethyl pyrocarbonate-treated water and treated with 20 U of RNase-free DNase (Gibco-BRL, Montreal, Quebec, Canada) at 37°C for 30 min to remove any DNA contamination.

    Article Title: Packaging of viral RNAs in virions of adenoviruses
    Article Snippet: To test this hypothesis, equal amounts (based on protein concentrations) of CsCl purified empty/intermediate and mature capsids of wt PAdV-3 were treated with RNase to remove RNAs contaminated outside of viral particles before processing for the isolation of virion RNAs as previously described [ ]. .. The isolated virion RNAs were treated with RNase-free DNase (Ambion) to eliminate the contaminated viral genomic DNA, followed by addition of 0.1 volume DNase inactivation reagent (DNA-free kit, Ambion).

    Polymerase Chain Reaction:

    Article Title: The Staphylococcus-Specific Gene rsr Represses agr and Virulence in Staphylococcus aureus ▿
    Article Snippet: RNA, prepared as described above, was treated with RNase-free DNase to remove residual DNA, according to the manufacturer's instructions (Turbo-DNAfree; Applied Biosystems/Ambion, Austin, TX). .. Successful removal of the DNA was confirmed by PCR analysis.

    Article Title: Impact of DNA extraction method and targeted 16S-rRNA hypervariable region on oral microbiota profiling
    Article Snippet: Three biological replicates of 10-fold serially diluted DNA standards from the PCR products of Prevotella Veroralis JCM6290 was used for the standard curve generation to quantify total bacteria. .. Each qPCR was performed in a reaction volume of 20 μl containing 0.5 μM of each primer (Bac-16s-F2: 5′-TTAAACTCAAAGGAATTGACGG, Bac-16s-R2: 5′-CTCACGRCACGAGCTGACGAC) , 1 μl of genomic DNA, 10 μl of 2 × SYBR Premix EXTaq mix (Takara) and 5 μl sterilized DNase-RNase-free water in MicroAmp fast optical 96-well reaction plates (Applied Biosystems, USA) with adhesive sealing.

    Article Title: Treponema pallidum Major Sheath Protein Homologue Tpr K Is a Target of Opsonic Antibody and the Protective Immune Response
    Article Snippet: Paragraph title: Transcriptional Analysis of the tpr Genes by Reverse Transcription PCR. ... Total RNA was extracted from a known number of freshly harvested treponemes followed by treatment of the RNA sample with RNAse free DNAse A ( GIBCO BRL ).

    Article Title: Release of targeted p53 from the mitochondrion as an early signal during mitochondrial dysfunction
    Article Snippet: After an 1 h RNase-free DNase digestion, 2μg of RNA was reversed transcribed with Superscript II Reverse transcriptase (Gibco-BRL, Gaithersburg, MD) at 42 °C in the presence of dithiothreitol (DTT), random primers, and 0.2 mM each dNTP. .. In each tube of Ready-to-go PCR beads (GE Healthcare Bio-sciences, Piscataway, NJ), 500 ng of cDNA and 2.5 μl of each primer were added, with a total volume of 25 μl.

    Lysis:

    Article Title: Hydrophobic Amino Acids in the Human Immunodeficiency Virus Type 1 p2 and Nucleocapsid Proteins Can Contribute to the Rescue of Deleted Viral RNA Packaging Signals
    Article Snippet: Viral RNA was dissolved in 50 μl of diethyl pyrocarbonate-treated water and treated with 20 U of RNase-free DNase (Gibco-BRL, Montreal, Quebec, Canada) at 37°C for 30 min to remove any DNA contamination. .. Transfected COS-7 cells were washed twice with cold phosphate-buffered saline and were lysed with NP-40 lysis buffer.

    Plasmid Preparation:

    Article Title: Artificial MicroRNAs Targeting C9orf72 Can Reduce Accumulation of Intra-nuclear Transcripts in ALS and FTD Patients
    Article Snippet: The cells were transfected on day 2 with 200 ng (G4 C2 )44 or (G4 C2 )3 plasmid. .. Optionally, cells were treated for 30 min with 5 mg/mL RNase A (QIAGEN) or 100 U RNase-free DNase (Invitrogen).

    RNA Extraction:

    Article Title: Hydrophobic Amino Acids in the Human Immunodeficiency Virus Type 1 p2 and Nucleocapsid Proteins Can Contribute to the Rescue of Deleted Viral RNA Packaging Signals
    Article Snippet: Viral RNA was purified from an amount of virus containing 2 ng of CA Ag using an RNA extraction kit (Qiagen). .. Viral RNA was dissolved in 50 μl of diethyl pyrocarbonate-treated water and treated with 20 U of RNase-free DNase (Gibco-BRL, Montreal, Quebec, Canada) at 37°C for 30 min to remove any DNA contamination.

    BAC Assay:

    Article Title: Impact of DNA extraction method and targeted 16S-rRNA hypervariable region on oral microbiota profiling
    Article Snippet: .. Each qPCR was performed in a reaction volume of 20 μl containing 0.5 μM of each primer (Bac-16s-F2: 5′-TTAAACTCAAAGGAATTGACGG, Bac-16s-R2: 5′-CTCACGRCACGAGCTGACGAC) , 1 μl of genomic DNA, 10 μl of 2 × SYBR Premix EXTaq mix (Takara) and 5 μl sterilized DNase-RNase-free water in MicroAmp fast optical 96-well reaction plates (Applied Biosystems, USA) with adhesive sealing. .. The qPCR parameters were 95 °C for 10 min, 45 cycles of three amplification steps including: 95 °C for 15 s, 60 °C for 20 s and 72 °C for 15 s, and final cooling at 25 °C for 1 min.

    Staining:

    Article Title: Use of SYTO 13, a fluorescent dye binding nucleic acids, for the detection of microparticles in in vitro systems
    Article Snippet: To assess staining of nucleic acids by SYTO 13 in MPs, cells were either fixed and permeabilized using the BD Cytofix/Cytoperm Kit (BD Biosciences, San Jose, CA) as per the manufacturer's instructions and then treated with nucleases, or treated with nucleases without permeabilization. .. This volume of the enriched and permeabilized MP suspension was treated with 100 U/ml of RNase-free DNase (Invitrogen Co., Carlsbad, CA) or 200 U/ml of DNase-free RNase (Sigma-Aldrich Co.) or both and incubated at 37°C for 90 min and then assessed for the presence of nucleic acid.

    Fluorescence In Situ Hybridization:

    Article Title: Artificial MicroRNAs Targeting C9orf72 Can Reduce Accumulation of Intra-nuclear Transcripts in ALS and FTD Patients
    Article Snippet: Paragraph title: RNA Foci FISH ... Optionally, cells were treated for 30 min with 5 mg/mL RNase A (QIAGEN) or 100 U RNase-free DNase (Invitrogen).

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  • 94
    Thermo Fisher p5rhh
    <t>p5RHH:</t> Nox4 siRNA nanocomplex transfection downregulates Nox4 mRNA and protein expression in VSMC from 4-month old wild-type mice. (A) p5RHH: Nox4 siRNA nanocomplex transfection efficiency compared to RNAiMAX Nox4 siRNA using quantitative RT-PCR (mean ±
    P5rhh, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p5rhh/product/Thermo Fisher
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    p5rhh - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    99
    Thermo Fisher rnase free water
    Analysis of 5' ends of leuX ]. All E. coli strains used in this study were derived from MG1693 ( rph-1) , which was considered wild-type. MG1693 has a single base pair deletion in the rph ]. The genotypes of the strains are noted at the top of the lane. Total <t>RNA</t> isolated from all the strains were reverse transcribed using a 32 P-end labelled leuX tRNA specific primer and the cDNAs were separated on a 6% polyacrylamide sequencing gel. A leuX DNA sequencing ladder (CTAG) generated by same leuX tRNA specific primer was also separated along with the cDNAs and was used to identify the transcription initiation site (I) and the mature 5' end (II). The primer extension analysis also identified several <t>RNase</t> E cleavage sites (indicated as *) in the rnpA49 mutant, which disappeared in the rne-1 rnpA49 strain.
    Rnase Free Water, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 357 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase free water/product/Thermo Fisher
    Average 99 stars, based on 357 article reviews
    Price from $9.99 to $1999.99
    rnase free water - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    92
    Thermo Fisher • rnase dnase free water • agarose • nucleic acid gel stain • tris base
    Analysis of 5' ends of leuX ]. All E. coli strains used in this study were derived from MG1693 ( rph-1) , which was considered wild-type. MG1693 has a single base pair deletion in the rph ]. The genotypes of the strains are noted at the top of the lane. Total <t>RNA</t> isolated from all the strains were reverse transcribed using a 32 P-end labelled leuX tRNA specific primer and the cDNAs were separated on a 6% polyacrylamide sequencing gel. A leuX DNA sequencing ladder (CTAG) generated by same leuX tRNA specific primer was also separated along with the cDNAs and was used to identify the transcription initiation site (I) and the mature 5' end (II). The primer extension analysis also identified several <t>RNase</t> E cleavage sites (indicated as *) in the rnpA49 mutant, which disappeared in the rne-1 rnpA49 strain.
    • Rnase Dnase Free Water • Agarose • Nucleic Acid Gel Stain • Tris Base, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/• rnase dnase free water • agarose • nucleic acid gel stain • tris base/product/Thermo Fisher
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    • rnase dnase free water • agarose • nucleic acid gel stain • tris base - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    99
    Thermo Fisher rnase dnase free water
    Analysis of 5' ends of leuX ]. All E. coli strains used in this study were derived from MG1693 ( rph-1) , which was considered wild-type. MG1693 has a single base pair deletion in the rph ]. The genotypes of the strains are noted at the top of the lane. Total <t>RNA</t> isolated from all the strains were reverse transcribed using a 32 P-end labelled leuX tRNA specific primer and the cDNAs were separated on a 6% polyacrylamide sequencing gel. A leuX DNA sequencing ladder (CTAG) generated by same leuX tRNA specific primer was also separated along with the cDNAs and was used to identify the transcription initiation site (I) and the mature 5' end (II). The primer extension analysis also identified several <t>RNase</t> E cleavage sites (indicated as *) in the rnpA49 mutant, which disappeared in the rne-1 rnpA49 strain.
    Rnase Dnase Free Water, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    p5RHH: Nox4 siRNA nanocomplex transfection downregulates Nox4 mRNA and protein expression in VSMC from 4-month old wild-type mice. (A) p5RHH: Nox4 siRNA nanocomplex transfection efficiency compared to RNAiMAX Nox4 siRNA using quantitative RT-PCR (mean ±

    Journal: Journal of molecular and cellular cardiology

    Article Title: NADPH oxidase 4 regulates vascular inflammation in aging and atherosclerosis

    doi: 10.1016/j.yjmcc.2016.12.004

    Figure Lengend Snippet: p5RHH: Nox4 siRNA nanocomplex transfection downregulates Nox4 mRNA and protein expression in VSMC from 4-month old wild-type mice. (A) p5RHH: Nox4 siRNA nanocomplex transfection efficiency compared to RNAiMAX Nox4 siRNA using quantitative RT-PCR (mean ±

    Article Snippet: Peptide-siRNA nanocomplexes were prepared by dilution of p5RHH (10 mM stock in DNAse-, RNAse-, and protease-free water (Sigma-Al-drich)) and siRNA (100 µM stock in siRNA buffer (Thermo Scientific)) 1:200 in Opti-MEM medium, followed by incubation for 40 min at 37 °C.

    Techniques: Transfection, Expressing, Mouse Assay, Quantitative RT-PCR

    p5RHH: Nox4 siRNA nanocomplex transfection downregulates H 2 O 2 production and shows low cytotoxicity in VSMCs from 4-month old wild-type mice. (A) H 2 O 2 production assayed with Amplex Red in media after 24 h incubation of VSMCs with TGFβ1 (mean ±

    Journal: Journal of molecular and cellular cardiology

    Article Title: NADPH oxidase 4 regulates vascular inflammation in aging and atherosclerosis

    doi: 10.1016/j.yjmcc.2016.12.004

    Figure Lengend Snippet: p5RHH: Nox4 siRNA nanocomplex transfection downregulates H 2 O 2 production and shows low cytotoxicity in VSMCs from 4-month old wild-type mice. (A) H 2 O 2 production assayed with Amplex Red in media after 24 h incubation of VSMCs with TGFβ1 (mean ±

    Article Snippet: Peptide-siRNA nanocomplexes were prepared by dilution of p5RHH (10 mM stock in DNAse-, RNAse-, and protease-free water (Sigma-Al-drich)) and siRNA (100 µM stock in siRNA buffer (Thermo Scientific)) 1:200 in Opti-MEM medium, followed by incubation for 40 min at 37 °C.

    Techniques: Transfection, Mouse Assay, Incubation

    p5RHH: Nox4 siRNA nanocomplex transfection of VSMCs attenuates TGFβ1-stimulated expression of pro-inflammatory genes from 4-month old wild-type mice. Relative mRNA expression was assessed by quantitative RT-PCR (mean ± SEM, n = 4); (A)

    Journal: Journal of molecular and cellular cardiology

    Article Title: NADPH oxidase 4 regulates vascular inflammation in aging and atherosclerosis

    doi: 10.1016/j.yjmcc.2016.12.004

    Figure Lengend Snippet: p5RHH: Nox4 siRNA nanocomplex transfection of VSMCs attenuates TGFβ1-stimulated expression of pro-inflammatory genes from 4-month old wild-type mice. Relative mRNA expression was assessed by quantitative RT-PCR (mean ± SEM, n = 4); (A)

    Article Snippet: Peptide-siRNA nanocomplexes were prepared by dilution of p5RHH (10 mM stock in DNAse-, RNAse-, and protease-free water (Sigma-Al-drich)) and siRNA (100 µM stock in siRNA buffer (Thermo Scientific)) 1:200 in Opti-MEM medium, followed by incubation for 40 min at 37 °C.

    Techniques: Transfection, Expressing, Mouse Assay, Quantitative RT-PCR

    TAK1, AP1, and canonical NFκB pathways regulate Nox4 expression in VSMCs from 4-month old mice. Relative Nox4 mRNA expression was assessed by quantitative RT-PCR (mean ± SEM, n = 4) in cells transfected with p5RHH:siRNA nanoparticles;

    Journal: Journal of molecular and cellular cardiology

    Article Title: NADPH oxidase 4 regulates vascular inflammation in aging and atherosclerosis

    doi: 10.1016/j.yjmcc.2016.12.004

    Figure Lengend Snippet: TAK1, AP1, and canonical NFκB pathways regulate Nox4 expression in VSMCs from 4-month old mice. Relative Nox4 mRNA expression was assessed by quantitative RT-PCR (mean ± SEM, n = 4) in cells transfected with p5RHH:siRNA nanoparticles;

    Article Snippet: Peptide-siRNA nanocomplexes were prepared by dilution of p5RHH (10 mM stock in DNAse-, RNAse-, and protease-free water (Sigma-Al-drich)) and siRNA (100 µM stock in siRNA buffer (Thermo Scientific)) 1:200 in Opti-MEM medium, followed by incubation for 40 min at 37 °C.

    Techniques: Expressing, Mouse Assay, Quantitative RT-PCR, Transfection

    Analysis of 5' ends of leuX ]. All E. coli strains used in this study were derived from MG1693 ( rph-1) , which was considered wild-type. MG1693 has a single base pair deletion in the rph ]. The genotypes of the strains are noted at the top of the lane. Total RNA isolated from all the strains were reverse transcribed using a 32 P-end labelled leuX tRNA specific primer and the cDNAs were separated on a 6% polyacrylamide sequencing gel. A leuX DNA sequencing ladder (CTAG) generated by same leuX tRNA specific primer was also separated along with the cDNAs and was used to identify the transcription initiation site (I) and the mature 5' end (II). The primer extension analysis also identified several RNase E cleavage sites (indicated as *) in the rnpA49 mutant, which disappeared in the rne-1 rnpA49 strain.

    Journal: Methods (San Diego, Calif.)

    Article Title: Analysis of post-transcriptional RNA metabolism in prokaryotes

    doi: 10.1016/j.ymeth.2018.11.006

    Figure Lengend Snippet: Analysis of 5' ends of leuX ]. All E. coli strains used in this study were derived from MG1693 ( rph-1) , which was considered wild-type. MG1693 has a single base pair deletion in the rph ]. The genotypes of the strains are noted at the top of the lane. Total RNA isolated from all the strains were reverse transcribed using a 32 P-end labelled leuX tRNA specific primer and the cDNAs were separated on a 6% polyacrylamide sequencing gel. A leuX DNA sequencing ladder (CTAG) generated by same leuX tRNA specific primer was also separated along with the cDNAs and was used to identify the transcription initiation site (I) and the mature 5' end (II). The primer extension analysis also identified several RNase E cleavage sites (indicated as *) in the rnpA49 mutant, which disappeared in the rne-1 rnpA49 strain.

    Article Snippet: The RNA is then resuspended in 10.5 μl of RNase-free water and reverse transcribed using Superscript™ III reverse transcriptase (Thermo Fisher) as described in .

    Techniques: Derivative Assay, Isolation, Sequencing, DNA Sequencing, Generated, Mutagenesis

    Steady-state RNA isolated from a wild-type and RNase III deletion ( Δrnc ) strains using the RNA snap ™ method. Total RNA (500 ng/lane) was separated on a 1% agarose gel and visualized with ethidium bromide staining. The various rRNA species are indicated to the right of the gel. The presence of the 30S primary rRNA transcript can easily be distinguished in the Δrnc mutant.

    Journal: Methods (San Diego, Calif.)

    Article Title: Analysis of post-transcriptional RNA metabolism in prokaryotes

    doi: 10.1016/j.ymeth.2018.11.006

    Figure Lengend Snippet: Steady-state RNA isolated from a wild-type and RNase III deletion ( Δrnc ) strains using the RNA snap ™ method. Total RNA (500 ng/lane) was separated on a 1% agarose gel and visualized with ethidium bromide staining. The various rRNA species are indicated to the right of the gel. The presence of the 30S primary rRNA transcript can easily be distinguished in the Δrnc mutant.

    Article Snippet: The RNA is then resuspended in 10.5 μl of RNase-free water and reverse transcribed using Superscript™ III reverse transcriptase (Thermo Fisher) as described in .

    Techniques: Isolation, Agarose Gel Electrophoresis, Staining, Mutagenesis