dnase on column digestion  (Qiagen)


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    Structured Review

    Qiagen dnase on column digestion
    Dnase On Column Digestion, supplied by Qiagen, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase on column digestion/product/Qiagen
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    dnase on column digestion - by Bioz Stars, 2020-01
    92/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Mutations in Kelch-like 3 and Cullin 3 cause hypertension and electrolyte abnormalities
    Article Snippet: Products were cloned into the pcDNA6.2/GW/D-TOPO mammalian expression vector (Invitrogen), and plasmids were purified (QIAprep, Qiagen) and sequenced. .. RNA was isolated using RNeasy with DNase on-column digestion (Qiagen).

    Transfection:

    Article Title: Mutations in Kelch-like 3 and Cullin 3 cause hypertension and electrolyte abnormalities
    Article Snippet: HEK293 cells were transfected independently with each plasmid using Lipofectamine 2000 (Invitrogen) and harvested ~24 hours post-transfection. .. RNA was isolated using RNeasy with DNase on-column digestion (Qiagen).

    Amplification:

    Article Title: Mutations in Kelch-like 3 and Cullin 3 cause hypertension and electrolyte abnormalities
    Article Snippet: Splicing assay A 3782 bp segment of CUL3 , extending from 287 bp proximal to exon 8 to 327 bp distal to exon 10, was amplified by PCR (Advantage 2 polymerase, Clontech) from genomic DNA of nine PHAII patients with different CUL3 mutations and one subject with wild-type CUL3 sequence. .. RNA was isolated using RNeasy with DNase on-column digestion (Qiagen).

    RNA Sequencing Assay:

    Article Title: The Lungfish Transcriptome: A Glimpse into Molecular Evolution Events at the Transition from Water to Land
    Article Snippet: Brain, liver and gonads were employed for total RNA isolation using either the RNeasy Midi Kit with DNase on-column digestion (Qiagen, Germany) or the Trizol protocol (TRIzol® Reagent, Life Technologies, USA), followed by DNase digestion of 30 μg RNA with 15 U DNase I (ThermoScientific, USA) in a total volume of 100 μl containing 100 U RNase Inhibitor (RiboLock, ThermoScientific, USA) for 1 h at 37 °C. .. One immature and one mature testis, three ovaries, and a brain and a liver each from a male and a female specimen ( ) were processed for RNA sequencing.

    Isolation:

    Article Title: Low wnt/β-catenin signaling determines leaky vessels in the subfornical organ and affects water homeostasis in mice
    Article Snippet: .. RNA isolation, transcription and real time PCR analysis RNA isolation was done using the RNeasy plus Microkit (Qiagen) according to the manufacturer recommendations with DNase on-column digestion (Qiagen) like suggested in the RNeasy Minikit (Quiagen). .. For cDNA synthesis (RevertAidTM H minus first strand cDNA synthesis kit, #K1632, Thermo Fisher Scientific) 57 ng RNA were used from SFO tissue of β-catenin GOF (Cre+ ) and control (Cre- ) mice.

    Article Title: Mutations in Kelch-like 3 and Cullin 3 cause hypertension and electrolyte abnormalities
    Article Snippet: .. RNA was isolated using RNeasy with DNase on-column digestion (Qiagen). .. The spliced expression products were assessed by reverse transcription with oligo(dT) priming (Superscript III RT, Invitrogen) followed by PCR with vector-specific and CUL3 -specific primers.

    Article Title: The Lungfish Transcriptome: A Glimpse into Molecular Evolution Events at the Transition from Water to Land
    Article Snippet: .. Brain, liver and gonads were employed for total RNA isolation using either the RNeasy Midi Kit with DNase on-column digestion (Qiagen, Germany) or the Trizol protocol (TRIzol® Reagent, Life Technologies, USA), followed by DNase digestion of 30 μg RNA with 15 U DNase I (ThermoScientific, USA) in a total volume of 100 μl containing 100 U RNase Inhibitor (RiboLock, ThermoScientific, USA) for 1 h at 37 °C. .. This step was followed by phenol/chloroform extraction or DNase in-solution digestion and column purification using RNeasy MinElute Cleanup Kit (Qiagen, Germany).

    RNA Extraction:

    Article Title: The Lungfish Transcriptome: A Glimpse into Molecular Evolution Events at the Transition from Water to Land
    Article Snippet: Paragraph title: Tissue sampling and RNA extraction ... Brain, liver and gonads were employed for total RNA isolation using either the RNeasy Midi Kit with DNase on-column digestion (Qiagen, Germany) or the Trizol protocol (TRIzol® Reagent, Life Technologies, USA), followed by DNase digestion of 30 μg RNA with 15 U DNase I (ThermoScientific, USA) in a total volume of 100 μl containing 100 U RNase Inhibitor (RiboLock, ThermoScientific, USA) for 1 h at 37 °C.

    Quantitative RT-PCR:

    Article Title: Low wnt/β-catenin signaling determines leaky vessels in the subfornical organ and affects water homeostasis in mice
    Article Snippet: RNA isolation, transcription and real time PCR analysis RNA isolation was done using the RNeasy plus Microkit (Qiagen) according to the manufacturer recommendations with DNase on-column digestion (Qiagen) like suggested in the RNeasy Minikit (Quiagen). .. Quantitative real time RT-PCR (qRT-PCR) was performed in technical triplicates for each sample using the Absolute qPCR SYBR Green Fluorescein Mix (AB-1219, Thermo Fisher Scientific) according to the manufacturer's protocol.

    Mouse Assay:

    Article Title: Low wnt/β-catenin signaling determines leaky vessels in the subfornical organ and affects water homeostasis in mice
    Article Snippet: RNA isolation, transcription and real time PCR analysis RNA isolation was done using the RNeasy plus Microkit (Qiagen) according to the manufacturer recommendations with DNase on-column digestion (Qiagen) like suggested in the RNeasy Minikit (Quiagen). .. For cDNA synthesis (RevertAidTM H minus first strand cDNA synthesis kit, #K1632, Thermo Fisher Scientific) 57 ng RNA were used from SFO tissue of β-catenin GOF (Cre+ ) and control (Cre- ) mice.

    SYBR Green Assay:

    Article Title: Low wnt/β-catenin signaling determines leaky vessels in the subfornical organ and affects water homeostasis in mice
    Article Snippet: RNA isolation, transcription and real time PCR analysis RNA isolation was done using the RNeasy plus Microkit (Qiagen) according to the manufacturer recommendations with DNase on-column digestion (Qiagen) like suggested in the RNeasy Minikit (Quiagen). .. Quantitative real time RT-PCR (qRT-PCR) was performed in technical triplicates for each sample using the Absolute qPCR SYBR Green Fluorescein Mix (AB-1219, Thermo Fisher Scientific) according to the manufacturer's protocol.

    Polymerase Chain Reaction:

    Article Title: Mutations in Kelch-like 3 and Cullin 3 cause hypertension and electrolyte abnormalities
    Article Snippet: Splicing assay A 3782 bp segment of CUL3 , extending from 287 bp proximal to exon 8 to 327 bp distal to exon 10, was amplified by PCR (Advantage 2 polymerase, Clontech) from genomic DNA of nine PHAII patients with different CUL3 mutations and one subject with wild-type CUL3 sequence. .. RNA was isolated using RNeasy with DNase on-column digestion (Qiagen).

    Purification:

    Article Title: Mutations in Kelch-like 3 and Cullin 3 cause hypertension and electrolyte abnormalities
    Article Snippet: Products were cloned into the pcDNA6.2/GW/D-TOPO mammalian expression vector (Invitrogen), and plasmids were purified (QIAprep, Qiagen) and sequenced. .. RNA was isolated using RNeasy with DNase on-column digestion (Qiagen).

    Article Title: The Lungfish Transcriptome: A Glimpse into Molecular Evolution Events at the Transition from Water to Land
    Article Snippet: Brain, liver and gonads were employed for total RNA isolation using either the RNeasy Midi Kit with DNase on-column digestion (Qiagen, Germany) or the Trizol protocol (TRIzol® Reagent, Life Technologies, USA), followed by DNase digestion of 30 μg RNA with 15 U DNase I (ThermoScientific, USA) in a total volume of 100 μl containing 100 U RNase Inhibitor (RiboLock, ThermoScientific, USA) for 1 h at 37 °C. .. This step was followed by phenol/chloroform extraction or DNase in-solution digestion and column purification using RNeasy MinElute Cleanup Kit (Qiagen, Germany).

    Expressing:

    Article Title: Mutations in Kelch-like 3 and Cullin 3 cause hypertension and electrolyte abnormalities
    Article Snippet: Products were cloned into the pcDNA6.2/GW/D-TOPO mammalian expression vector (Invitrogen), and plasmids were purified (QIAprep, Qiagen) and sequenced. .. RNA was isolated using RNeasy with DNase on-column digestion (Qiagen).

    Sequencing:

    Article Title: Mutations in Kelch-like 3 and Cullin 3 cause hypertension and electrolyte abnormalities
    Article Snippet: Splicing assay A 3782 bp segment of CUL3 , extending from 287 bp proximal to exon 8 to 327 bp distal to exon 10, was amplified by PCR (Advantage 2 polymerase, Clontech) from genomic DNA of nine PHAII patients with different CUL3 mutations and one subject with wild-type CUL3 sequence. .. RNA was isolated using RNeasy with DNase on-column digestion (Qiagen).

    Article Title: The Lungfish Transcriptome: A Glimpse into Molecular Evolution Events at the Transition from Water to Land
    Article Snippet: Species identity was confirmed by partial sequencing of the 16 S gene essentially as described by Tokita et al. , except that the annealing temperature was set to 56 °C. .. Brain, liver and gonads were employed for total RNA isolation using either the RNeasy Midi Kit with DNase on-column digestion (Qiagen, Germany) or the Trizol protocol (TRIzol® Reagent, Life Technologies, USA), followed by DNase digestion of 30 μg RNA with 15 U DNase I (ThermoScientific, USA) in a total volume of 100 μl containing 100 U RNase Inhibitor (RiboLock, ThermoScientific, USA) for 1 h at 37 °C.

    Real-time Polymerase Chain Reaction:

    Article Title: Low wnt/β-catenin signaling determines leaky vessels in the subfornical organ and affects water homeostasis in mice
    Article Snippet: .. RNA isolation, transcription and real time PCR analysis RNA isolation was done using the RNeasy plus Microkit (Qiagen) according to the manufacturer recommendations with DNase on-column digestion (Qiagen) like suggested in the RNeasy Minikit (Quiagen). .. For cDNA synthesis (RevertAidTM H minus first strand cDNA synthesis kit, #K1632, Thermo Fisher Scientific) 57 ng RNA were used from SFO tissue of β-catenin GOF (Cre+ ) and control (Cre- ) mice.

    Sampling:

    Article Title: The Lungfish Transcriptome: A Glimpse into Molecular Evolution Events at the Transition from Water to Land
    Article Snippet: Paragraph title: Tissue sampling and RNA extraction ... Brain, liver and gonads were employed for total RNA isolation using either the RNeasy Midi Kit with DNase on-column digestion (Qiagen, Germany) or the Trizol protocol (TRIzol® Reagent, Life Technologies, USA), followed by DNase digestion of 30 μg RNA with 15 U DNase I (ThermoScientific, USA) in a total volume of 100 μl containing 100 U RNase Inhibitor (RiboLock, ThermoScientific, USA) for 1 h at 37 °C.

    Fluorescence In Situ Hybridization:

    Article Title: The Lungfish Transcriptome: A Glimpse into Molecular Evolution Events at the Transition from Water to Land
    Article Snippet: Tissue sampling and RNA extraction Male and female P. annectens juveniles were obtained from a local fish importer. .. Brain, liver and gonads were employed for total RNA isolation using either the RNeasy Midi Kit with DNase on-column digestion (Qiagen, Germany) or the Trizol protocol (TRIzol® Reagent, Life Technologies, USA), followed by DNase digestion of 30 μg RNA with 15 U DNase I (ThermoScientific, USA) in a total volume of 100 μl containing 100 U RNase Inhibitor (RiboLock, ThermoScientific, USA) for 1 h at 37 °C.

    Splicing Assay:

    Article Title: Mutations in Kelch-like 3 and Cullin 3 cause hypertension and electrolyte abnormalities
    Article Snippet: Paragraph title: Splicing assay ... RNA was isolated using RNeasy with DNase on-column digestion (Qiagen).

    Plasmid Preparation:

    Article Title: Mutations in Kelch-like 3 and Cullin 3 cause hypertension and electrolyte abnormalities
    Article Snippet: HEK293 cells were transfected independently with each plasmid using Lipofectamine 2000 (Invitrogen) and harvested ~24 hours post-transfection. .. RNA was isolated using RNeasy with DNase on-column digestion (Qiagen).

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  • 90
    Qiagen rnase free dnase set
    Rnase Free Dnase Set, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 962 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase free dnase set/product/Qiagen
    Average 90 stars, based on 962 article reviews
    Price from $9.99 to $1999.99
    rnase free dnase set - by Bioz Stars, 2020-01
    90/100 stars
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