dnase i endonuclease  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Thermo Fisher dnase i endonuclease
    Effect of calpain-like silencing during PCD. (A) DNA fragmentation in calpain-like silenced trophozoites after PCD induction by G418. Confocal microscopy analysis of trophozoites showing the TUNEL staining, samples were counter-stained with DAPI. (A) Negative control, untreated trophozoites; (B) Positive control, trophozoites treated with 20 μg of <t>DNase</t> I endonuclease for 30 min; (C) Trophozoites after a 9-h incubation with 10 μg/ml of G418; (D,E) Trophozoites pre-incubated for 24 h with NRS (D) or calpain-like (E) siRNAs sequences and then 9 h with 10 μg/ml G418; (B) Densitometric analysis of (A). (C) Effect of calpain-like silencing on the cell viability of E. histolytica incubated with G418; (A) Negative control, untreated trophozoites; (B) Trophozoites after a 9-h incubation with 10 μg/ml of G418; (C,D) Trophozoites pre-incubated for 24 h with NRS (C) or calpain-like (D) siRNAs sequences and then 9 h with 10 μg/ml G418. *indicate statistically significant difference: P
    Dnase I Endonuclease, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase i endonuclease/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    dnase i endonuclease - by Bioz Stars, 2020-03
    99/100 stars

    Images

    1) Product Images from "A Calpain-Like Protein Is Involved in the Execution Phase of Programmed Cell Death of Entamoeba histolytica"

    Article Title: A Calpain-Like Protein Is Involved in the Execution Phase of Programmed Cell Death of Entamoeba histolytica

    Journal: Frontiers in Cellular and Infection Microbiology

    doi: 10.3389/fcimb.2018.00339

    Effect of calpain-like silencing during PCD. (A) DNA fragmentation in calpain-like silenced trophozoites after PCD induction by G418. Confocal microscopy analysis of trophozoites showing the TUNEL staining, samples were counter-stained with DAPI. (A) Negative control, untreated trophozoites; (B) Positive control, trophozoites treated with 20 μg of DNase I endonuclease for 30 min; (C) Trophozoites after a 9-h incubation with 10 μg/ml of G418; (D,E) Trophozoites pre-incubated for 24 h with NRS (D) or calpain-like (E) siRNAs sequences and then 9 h with 10 μg/ml G418; (B) Densitometric analysis of (A). (C) Effect of calpain-like silencing on the cell viability of E. histolytica incubated with G418; (A) Negative control, untreated trophozoites; (B) Trophozoites after a 9-h incubation with 10 μg/ml of G418; (C,D) Trophozoites pre-incubated for 24 h with NRS (C) or calpain-like (D) siRNAs sequences and then 9 h with 10 μg/ml G418. *indicate statistically significant difference: P
    Figure Legend Snippet: Effect of calpain-like silencing during PCD. (A) DNA fragmentation in calpain-like silenced trophozoites after PCD induction by G418. Confocal microscopy analysis of trophozoites showing the TUNEL staining, samples were counter-stained with DAPI. (A) Negative control, untreated trophozoites; (B) Positive control, trophozoites treated with 20 μg of DNase I endonuclease for 30 min; (C) Trophozoites after a 9-h incubation with 10 μg/ml of G418; (D,E) Trophozoites pre-incubated for 24 h with NRS (D) or calpain-like (E) siRNAs sequences and then 9 h with 10 μg/ml G418; (B) Densitometric analysis of (A). (C) Effect of calpain-like silencing on the cell viability of E. histolytica incubated with G418; (A) Negative control, untreated trophozoites; (B) Trophozoites after a 9-h incubation with 10 μg/ml of G418; (C,D) Trophozoites pre-incubated for 24 h with NRS (C) or calpain-like (D) siRNAs sequences and then 9 h with 10 μg/ml G418. *indicate statistically significant difference: P

    Techniques Used: Confocal Microscopy, TUNEL Assay, Staining, Negative Control, Positive Control, Incubation

    2) Product Images from "A Calpain-Like Protein Is Involved in the Execution Phase of Programmed Cell Death of Entamoeba histolytica"

    Article Title: A Calpain-Like Protein Is Involved in the Execution Phase of Programmed Cell Death of Entamoeba histolytica

    Journal: Frontiers in Cellular and Infection Microbiology

    doi: 10.3389/fcimb.2018.00339

    Effect of calpain-like silencing during PCD. (A) DNA fragmentation in calpain-like silenced trophozoites after PCD induction by G418. Confocal microscopy analysis of trophozoites showing the TUNEL staining, samples were counter-stained with DAPI. (A) Negative control, untreated trophozoites; (B) Positive control, trophozoites treated with 20 μg of DNase I endonuclease for 30 min; (C) Trophozoites after a 9-h incubation with 10 μg/ml of G418; (D,E) Trophozoites pre-incubated for 24 h with NRS (D) or calpain-like (E) siRNAs sequences and then 9 h with 10 μg/ml G418; (B) Densitometric analysis of (A). (C) Effect of calpain-like silencing on the cell viability of E. histolytica incubated with G418; (A) Negative control, untreated trophozoites; (B) Trophozoites after a 9-h incubation with 10 μg/ml of G418; (C,D) Trophozoites pre-incubated for 24 h with NRS (C) or calpain-like (D) siRNAs sequences and then 9 h with 10 μg/ml G418. *indicate statistically significant difference: P
    Figure Legend Snippet: Effect of calpain-like silencing during PCD. (A) DNA fragmentation in calpain-like silenced trophozoites after PCD induction by G418. Confocal microscopy analysis of trophozoites showing the TUNEL staining, samples were counter-stained with DAPI. (A) Negative control, untreated trophozoites; (B) Positive control, trophozoites treated with 20 μg of DNase I endonuclease for 30 min; (C) Trophozoites after a 9-h incubation with 10 μg/ml of G418; (D,E) Trophozoites pre-incubated for 24 h with NRS (D) or calpain-like (E) siRNAs sequences and then 9 h with 10 μg/ml G418; (B) Densitometric analysis of (A). (C) Effect of calpain-like silencing on the cell viability of E. histolytica incubated with G418; (A) Negative control, untreated trophozoites; (B) Trophozoites after a 9-h incubation with 10 μg/ml of G418; (C,D) Trophozoites pre-incubated for 24 h with NRS (C) or calpain-like (D) siRNAs sequences and then 9 h with 10 μg/ml G418. *indicate statistically significant difference: P

    Techniques Used: Confocal Microscopy, TUNEL Assay, Staining, Negative Control, Positive Control, Incubation

    Related Articles

    TUNEL Assay:

    Article Title: A Calpain-Like Protein Is Involved in the Execution Phase of Programmed Cell Death of Entamoeba histolytica
    Article Snippet: Paragraph title: Terminal deoxynucleotidyltransferase-mediated dUTP nick-end labeling (TUNEL) assays ... As a positive control, trophozoites were treated with 20 mg/ml DNase I endonuclease (Invitrogen) for 30 min, and non-treated trophozoites were used as a negative control.

    MTT Assay:

    Article Title: Intracellular MicroRNA imaging using telomerase-catalyzed FRET ratioflares with signal amplification MicroRNA imaging using telomerase-catalyzed FRET ratioflares with signal amplification †Electronic supplementary information (ESI) available: Additional documentation (12 figures) including: characterizatio
    Article Snippet: HSA (human serum albumin), BSA (bovine serum albumin), Hcy (homocysteine), Cys (cysteine), GSH (glutathione), MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H -tetrazolium bromide), trisodium citrate, and HAuCl4 ·3H2 O (hydrogen tetrachloroaurate trihydrate) were purchased from Energy Chemical Reagent Company. .. Mercaptoethanol, EGCG (epigallocatechin gallate) and TCEP·HCl (tris(2-carboxyethyl)phosphine hydrochloride) were purchased from Solarbio Science & Technology Co., Ltd. Lipofectamine 3000 transfection reagent, Lipofectamine-RNAiMAX transfection reagent, and DNase I endonuclease were purchased from Life Technologies Co. (USA). dNTP (deoxyribonucleoside triphosphate) was obtained from TaKaRa Biotechnology Co., Ltd (Dalian, China).

    Transfection:

    Article Title: Intracellular MicroRNA imaging using telomerase-catalyzed FRET ratioflares with signal amplification MicroRNA imaging using telomerase-catalyzed FRET ratioflares with signal amplification †Electronic supplementary information (ESI) available: Additional documentation (12 figures) including: characterizatio
    Article Snippet: .. Mercaptoethanol, EGCG (epigallocatechin gallate) and TCEP·HCl (tris(2-carboxyethyl)phosphine hydrochloride) were purchased from Solarbio Science & Technology Co., Ltd. Lipofectamine 3000 transfection reagent, Lipofectamine-RNAiMAX transfection reagent, and DNase I endonuclease were purchased from Life Technologies Co. (USA). dNTP (deoxyribonucleoside triphosphate) was obtained from TaKaRa Biotechnology Co., Ltd (Dalian, China). .. Twice-distilled purified water obtained from Milli-Q systems was used in all experiments.

    Positive Control:

    Article Title: A Calpain-Like Protein Is Involved in the Execution Phase of Programmed Cell Death of Entamoeba histolytica
    Article Snippet: .. As a positive control, trophozoites were treated with 20 mg/ml DNase I endonuclease (Invitrogen) for 30 min, and non-treated trophozoites were used as a negative control. .. To determine the effect of calpain-like gene silencing on the viability of trophozoites subjected to PCD, cells were stained with propidium iodide (PI) and analyzed by flow cytometry.

    Fluorescence:

    Article Title: Intracellular MicroRNA imaging using telomerase-catalyzed FRET ratioflares with signal amplification MicroRNA imaging using telomerase-catalyzed FRET ratioflares with signal amplification †Electronic supplementary information (ESI) available: Additional documentation (12 figures) including: characterizatio
    Article Snippet: Mercaptoethanol, EGCG (epigallocatechin gallate) and TCEP·HCl (tris(2-carboxyethyl)phosphine hydrochloride) were purchased from Solarbio Science & Technology Co., Ltd. Lipofectamine 3000 transfection reagent, Lipofectamine-RNAiMAX transfection reagent, and DNase I endonuclease were purchased from Life Technologies Co. (USA). dNTP (deoxyribonucleoside triphosphate) was obtained from TaKaRa Biotechnology Co., Ltd (Dalian, China). .. Fluorescence spectra were recorded on a Thermo Scientific NanoDrop 3300 Fluorospectrometer and VAEIAN CARY Eclipse fluorescence spectrophotometer (serial no. FL0812-M018), with slit widths modified to adjust the fluorescence intensity to a suitable range.

    Electron Microscopy:

    Article Title: Intracellular MicroRNA imaging using telomerase-catalyzed FRET ratioflares with signal amplification MicroRNA imaging using telomerase-catalyzed FRET ratioflares with signal amplification †Electronic supplementary information (ESI) available: Additional documentation (12 figures) including: characterizatio
    Article Snippet: Mercaptoethanol, EGCG (epigallocatechin gallate) and TCEP·HCl (tris(2-carboxyethyl)phosphine hydrochloride) were purchased from Solarbio Science & Technology Co., Ltd. Lipofectamine 3000 transfection reagent, Lipofectamine-RNAiMAX transfection reagent, and DNase I endonuclease were purchased from Life Technologies Co. (USA). dNTP (deoxyribonucleoside triphosphate) was obtained from TaKaRa Biotechnology Co., Ltd (Dalian, China). .. The morphological features of AuNPs were obtained by transmission electron microscopy (TEM, T20) at 300 kV.

    Labeling:

    Article Title: A Calpain-Like Protein Is Involved in the Execution Phase of Programmed Cell Death of Entamoeba histolytica
    Article Snippet: Paragraph title: Terminal deoxynucleotidyltransferase-mediated dUTP nick-end labeling (TUNEL) assays ... As a positive control, trophozoites were treated with 20 mg/ml DNase I endonuclease (Invitrogen) for 30 min, and non-treated trophozoites were used as a negative control.

    Negative Control:

    Article Title: A Calpain-Like Protein Is Involved in the Execution Phase of Programmed Cell Death of Entamoeba histolytica
    Article Snippet: .. As a positive control, trophozoites were treated with 20 mg/ml DNase I endonuclease (Invitrogen) for 30 min, and non-treated trophozoites were used as a negative control. .. To determine the effect of calpain-like gene silencing on the viability of trophozoites subjected to PCD, cells were stained with propidium iodide (PI) and analyzed by flow cytometry.

    Microscopy:

    Article Title: A Calpain-Like Protein Is Involved in the Execution Phase of Programmed Cell Death of Entamoeba histolytica
    Article Snippet: The samples were observed through a confocal microscope (Carl Zeiss LSM 700) using the ZEN 2009 software. .. As a positive control, trophozoites were treated with 20 mg/ml DNase I endonuclease (Invitrogen) for 30 min, and non-treated trophozoites were used as a negative control.

    Purification:

    Article Title: Intracellular MicroRNA imaging using telomerase-catalyzed FRET ratioflares with signal amplification MicroRNA imaging using telomerase-catalyzed FRET ratioflares with signal amplification †Electronic supplementary information (ESI) available: Additional documentation (12 figures) including: characterizatio
    Article Snippet: Mercaptoethanol, EGCG (epigallocatechin gallate) and TCEP·HCl (tris(2-carboxyethyl)phosphine hydrochloride) were purchased from Solarbio Science & Technology Co., Ltd. Lipofectamine 3000 transfection reagent, Lipofectamine-RNAiMAX transfection reagent, and DNase I endonuclease were purchased from Life Technologies Co. (USA). dNTP (deoxyribonucleoside triphosphate) was obtained from TaKaRa Biotechnology Co., Ltd (Dalian, China). .. Twice-distilled purified water obtained from Milli-Q systems was used in all experiments.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Intracellular MicroRNA imaging using telomerase-catalyzed FRET ratioflares with signal amplification MicroRNA imaging using telomerase-catalyzed FRET ratioflares with signal amplification †Electronic supplementary information (ESI) available: Additional documentation (12 figures) including: characterizatio
    Article Snippet: Human telomerase ELISA kit was purchased from Nanjing SenBeiJia Biological Technology Co., Ltd (Nanjing, China). .. Mercaptoethanol, EGCG (epigallocatechin gallate) and TCEP·HCl (tris(2-carboxyethyl)phosphine hydrochloride) were purchased from Solarbio Science & Technology Co., Ltd. Lipofectamine 3000 transfection reagent, Lipofectamine-RNAiMAX transfection reagent, and DNase I endonuclease were purchased from Life Technologies Co. (USA). dNTP (deoxyribonucleoside triphosphate) was obtained from TaKaRa Biotechnology Co., Ltd (Dalian, China).

    Incubation:

    Article Title: A Calpain-Like Protein Is Involved in the Execution Phase of Programmed Cell Death of Entamoeba histolytica
    Article Snippet: After the samples were washed twice with PBS, 50 μl of TUNEL reaction mixture (Roche) was added and incubated for 60 min at 37°C in a humidified atmosphere in the dark. .. As a positive control, trophozoites were treated with 20 mg/ml DNase I endonuclease (Invitrogen) for 30 min, and non-treated trophozoites were used as a negative control.

    Spectrophotometry:

    Article Title: Intracellular MicroRNA imaging using telomerase-catalyzed FRET ratioflares with signal amplification MicroRNA imaging using telomerase-catalyzed FRET ratioflares with signal amplification †Electronic supplementary information (ESI) available: Additional documentation (12 figures) including: characterizatio
    Article Snippet: Mercaptoethanol, EGCG (epigallocatechin gallate) and TCEP·HCl (tris(2-carboxyethyl)phosphine hydrochloride) were purchased from Solarbio Science & Technology Co., Ltd. Lipofectamine 3000 transfection reagent, Lipofectamine-RNAiMAX transfection reagent, and DNase I endonuclease were purchased from Life Technologies Co. (USA). dNTP (deoxyribonucleoside triphosphate) was obtained from TaKaRa Biotechnology Co., Ltd (Dalian, China). .. Fluorescence spectra were recorded on a Thermo Scientific NanoDrop 3300 Fluorospectrometer and VAEIAN CARY Eclipse fluorescence spectrophotometer (serial no. FL0812-M018), with slit widths modified to adjust the fluorescence intensity to a suitable range.

    Transmission Assay:

    Article Title: Intracellular MicroRNA imaging using telomerase-catalyzed FRET ratioflares with signal amplification MicroRNA imaging using telomerase-catalyzed FRET ratioflares with signal amplification †Electronic supplementary information (ESI) available: Additional documentation (12 figures) including: characterizatio
    Article Snippet: Mercaptoethanol, EGCG (epigallocatechin gallate) and TCEP·HCl (tris(2-carboxyethyl)phosphine hydrochloride) were purchased from Solarbio Science & Technology Co., Ltd. Lipofectamine 3000 transfection reagent, Lipofectamine-RNAiMAX transfection reagent, and DNase I endonuclease were purchased from Life Technologies Co. (USA). dNTP (deoxyribonucleoside triphosphate) was obtained from TaKaRa Biotechnology Co., Ltd (Dalian, China). .. The morphological features of AuNPs were obtained by transmission electron microscopy (TEM, T20) at 300 kV.

    Modification:

    Article Title: Intracellular MicroRNA imaging using telomerase-catalyzed FRET ratioflares with signal amplification MicroRNA imaging using telomerase-catalyzed FRET ratioflares with signal amplification †Electronic supplementary information (ESI) available: Additional documentation (12 figures) including: characterizatio
    Article Snippet: Mercaptoethanol, EGCG (epigallocatechin gallate) and TCEP·HCl (tris(2-carboxyethyl)phosphine hydrochloride) were purchased from Solarbio Science & Technology Co., Ltd. Lipofectamine 3000 transfection reagent, Lipofectamine-RNAiMAX transfection reagent, and DNase I endonuclease were purchased from Life Technologies Co. (USA). dNTP (deoxyribonucleoside triphosphate) was obtained from TaKaRa Biotechnology Co., Ltd (Dalian, China). .. Fluorescence spectra were recorded on a Thermo Scientific NanoDrop 3300 Fluorospectrometer and VAEIAN CARY Eclipse fluorescence spectrophotometer (serial no. FL0812-M018), with slit widths modified to adjust the fluorescence intensity to a suitable range.

    Transmission Electron Microscopy:

    Article Title: Intracellular MicroRNA imaging using telomerase-catalyzed FRET ratioflares with signal amplification MicroRNA imaging using telomerase-catalyzed FRET ratioflares with signal amplification †Electronic supplementary information (ESI) available: Additional documentation (12 figures) including: characterizatio
    Article Snippet: Mercaptoethanol, EGCG (epigallocatechin gallate) and TCEP·HCl (tris(2-carboxyethyl)phosphine hydrochloride) were purchased from Solarbio Science & Technology Co., Ltd. Lipofectamine 3000 transfection reagent, Lipofectamine-RNAiMAX transfection reagent, and DNase I endonuclease were purchased from Life Technologies Co. (USA). dNTP (deoxyribonucleoside triphosphate) was obtained from TaKaRa Biotechnology Co., Ltd (Dalian, China). .. The morphological features of AuNPs were obtained by transmission electron microscopy (TEM, T20) at 300 kV.

    Software:

    Article Title: A Calpain-Like Protein Is Involved in the Execution Phase of Programmed Cell Death of Entamoeba histolytica
    Article Snippet: The samples were observed through a confocal microscope (Carl Zeiss LSM 700) using the ZEN 2009 software. .. As a positive control, trophozoites were treated with 20 mg/ml DNase I endonuclease (Invitrogen) for 30 min, and non-treated trophozoites were used as a negative control.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher dnase i
    Autorepression of graTA promoter by GraA. (A) β-Galactosidase activities measured in P. putida wild-type (WT) and graTA deletion strains carrying the transcriptional fusion of the graTA promoter with lacZ in plasmid p9TT1586. Bacteria were grown overnight in LB medium at 30°C. Data (means with 95% confidence intervals) of at least three independent experiments are presented. (B) Promoter region of graTA . The −10 and −35 elements of the promoter are boxed, the transcriptional start site is indicated by a black arrow, and the ATG of graT is underlined. Brackets indicate the regions protected by <t>DNase</t> I cleavage by binding of GraA, and the asterisk marks a hypersensitive cleavage site. The palindromic sequences of GraA binding site are designated by gray arrows. (C) DNase I footprint analysis for determining GraA and GraT-GraA binding sites in the graTA promoter region. Footprints on the upper strand of the promoter region are presented. Lane 0 represents the DNase I reaction carried out in the absence of TA proteins. Other lanes represent reactions which contain increasing amounts (in pmol) of either the GraA-His or the His-GraT+GraA complex. The line on the right designates the region of protection against DNase I cleavage, and the asterisk signifies a base which becomes hypersensitive to DNase I cleavage upon the formation of the DNA complex with TA protein.
    Dnase I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase i/product/Thermo Fisher
    Average 99 stars, based on 46 article reviews
    Price from $9.99 to $1999.99
    dnase i - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher dnase i amplification grade
    NETs contribute to the pathogenesis of myocarditis. (a) Confocal immunofluorescence images of an EMB of patient 6 suffering from PVB19-positive myocarditis (see Table 1 ). Top: Images show staining of <t>H2A-H2B-DNA,</t> MPO, and H3Cit as well as the merged image. Bar, 20 µm. Bottom: Magnification of a selected area of the merged confocal image (box) from the top panel. Arrows indicate NETs. Bar, 10 µm. (b and c) EAM score (b) as well as evaluation of the heart/body weight ratio (c) at day 21 after induction of EAM or sham immunization (sham; mean EAM score, 0.5 ± 0.19; mean heart/body weight ratio, 0.57 ± 0.01). Mice were treated with <t>DNase</t> (EAM + DNase: mean EAM score, 0.8 ± 0.19; mean heart/body weight ratio, 0.62 ± 0.005), Cl-amid (EAM + Cl-amid: mean EAM score, 0.9 ± 0.20; mean heart/body weight ratio, 0.62 ± 0.01), or vehicle as control (EAM + vehicle: mean EAM score, 1.7 ± 0.25; mean heart/body weight ratio, 0.71 ± 0.04) as indicated. n = 8 for sham group; n = 26 for EAM groups. (d) Left: Representative confocal images of cardiac sections of immunized WT mice at day 21 after induction of EAM (EAM + vehicle). Images show staining of DNA, NE, and H3Cit as well as the merged image. Bars, 20 µm. Right: Representative confocal magnified image of NETs. Images show staining of DNA, NE, and H3Cit as well as the merged image, colocalization (coloc) of DNA/NE, and DNA/H3Cit as indicated. Bars, 10 µm. (e) Representative confocal images of cardiac sections of EAM mice at day 21 after induction of EAM and blockade of MK for 21 d. Images show staining of DNA, NE, and H3Cit as well as the merged image. Bars, 20 µm. (f–h) Histological analysis of extravasated PMN into the cardiac tissue at day 21 after induction of EAM without blocking MK (EAM + vehicle) or after application of an anti-N-MK blocking Ab (EAM + anti-N-MK). Diagrams show the number of extravasated PMNs per mm 2 (f: vehicle, 768 ± 55 PMN/mm 2 ; anti-N-MK, 158 ± 86 PMN/mm 2 ), the number of NET + PMNs per mm 2 (g: vehicle, 40 ± 6 NET + PMN; anti-N-MK = 5 ± 2 NET + PMN), and the number of NET + PMNs as a percentage of all extravasated PMNs (h: vehicle, 5.1 ± 0.5 NET + PMN [%]; anti-N-MK, 0.9 ± 0.6 NET + PMN [%]). n = 6 mice. To determine P values, ANOVA on ranks with Dunn’s multiple comparisons test was performed for c and d, and an unpaired Student's t test was used for f–h. For all panels, *, P
    Dnase I Amplification Grade, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase i amplification grade/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dnase i amplification grade - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    94
    Thermo Fisher human dnase i
    Enzymatic resistance of phosphorothioate-modified Au-TDNNs. (A, B) Fluorescence time graph depicting terminal-modified Au-TDNN and overall-modified Au-TDNN degradation by DNase I. (C, D) Fluorescence time graph depicting terminal-modified Au-TDNN and overall-modified Au-TDNN degradation by Exo III.
    Human Dnase I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human dnase i/product/Thermo Fisher
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    human dnase i - by Bioz Stars, 2020-03
    94/100 stars
      Buy from Supplier

    Image Search Results


    Autorepression of graTA promoter by GraA. (A) β-Galactosidase activities measured in P. putida wild-type (WT) and graTA deletion strains carrying the transcriptional fusion of the graTA promoter with lacZ in plasmid p9TT1586. Bacteria were grown overnight in LB medium at 30°C. Data (means with 95% confidence intervals) of at least three independent experiments are presented. (B) Promoter region of graTA . The −10 and −35 elements of the promoter are boxed, the transcriptional start site is indicated by a black arrow, and the ATG of graT is underlined. Brackets indicate the regions protected by DNase I cleavage by binding of GraA, and the asterisk marks a hypersensitive cleavage site. The palindromic sequences of GraA binding site are designated by gray arrows. (C) DNase I footprint analysis for determining GraA and GraT-GraA binding sites in the graTA promoter region. Footprints on the upper strand of the promoter region are presented. Lane 0 represents the DNase I reaction carried out in the absence of TA proteins. Other lanes represent reactions which contain increasing amounts (in pmol) of either the GraA-His or the His-GraT+GraA complex. The line on the right designates the region of protection against DNase I cleavage, and the asterisk signifies a base which becomes hypersensitive to DNase I cleavage upon the formation of the DNA complex with TA protein.

    Journal: Journal of Bacteriology

    Article Title: A Moderate Toxin, GraT, Modulates Growth Rate and Stress Tolerance of Pseudomonas putida

    doi: 10.1128/JB.00851-13

    Figure Lengend Snippet: Autorepression of graTA promoter by GraA. (A) β-Galactosidase activities measured in P. putida wild-type (WT) and graTA deletion strains carrying the transcriptional fusion of the graTA promoter with lacZ in plasmid p9TT1586. Bacteria were grown overnight in LB medium at 30°C. Data (means with 95% confidence intervals) of at least three independent experiments are presented. (B) Promoter region of graTA . The −10 and −35 elements of the promoter are boxed, the transcriptional start site is indicated by a black arrow, and the ATG of graT is underlined. Brackets indicate the regions protected by DNase I cleavage by binding of GraA, and the asterisk marks a hypersensitive cleavage site. The palindromic sequences of GraA binding site are designated by gray arrows. (C) DNase I footprint analysis for determining GraA and GraT-GraA binding sites in the graTA promoter region. Footprints on the upper strand of the promoter region are presented. Lane 0 represents the DNase I reaction carried out in the absence of TA proteins. Other lanes represent reactions which contain increasing amounts (in pmol) of either the GraA-His or the His-GraT+GraA complex. The line on the right designates the region of protection against DNase I cleavage, and the asterisk signifies a base which becomes hypersensitive to DNase I cleavage upon the formation of the DNA complex with TA protein.

    Article Snippet: Proteins were allowed to bind to DNA during 30 min at room temperature before the start of digestion by DNase I (0.06 U; Thermo Scientific) for 3 min.

    Techniques: Plasmid Preparation, Binding Assay

    Bacteria induce a unique algal death in aging co-cultures. ( a–d ) Images of cultures demonstrating the change in the culture color over time. ( e–h ) Phase contrast microscopy images. Arrow points to shed coccoliths. ( i–l ) Fluorescent images of chlorophyll and accessory pigments autofluorescence. ( m–p ) Fluorescent images of dead cells stained with Sytox green. Percentages indicate the number of dead cells counted in each population. For each value n  >  300 and the standard deviation between several analyzed fields was up to 20% of the indicated value. ( q–t ) Overlay of phase contrast microscopy images (grey) with fluorescent images of TUNEL assay (green) of cultures at day 20 (see Materials and methods). ( q ) Co-culture, ( r ) Axenic algal culture, ( s ) Positive control, cells were pretreated with DNase I, ( t ) Negative control, the terminal deoxynucleotidyl transferase enzyme (TdT) was replaced with distilled water. Percentages indicate the number of positively stained cells counted in each population. For each value n  >  300 and the standard deviation between several analyzed fields was up to 25% of the indicated value. Scale bar corresponds to 1 µm in e–p and 4 µm in q–t. DOI: http://dx.doi.org/10.7554/eLife.17473.009

    Journal: eLife

    Article Title: Dynamic metabolic exchange governs a marine algal-bacterial interaction

    doi: 10.7554/eLife.17473

    Figure Lengend Snippet: Bacteria induce a unique algal death in aging co-cultures. ( a–d ) Images of cultures demonstrating the change in the culture color over time. ( e–h ) Phase contrast microscopy images. Arrow points to shed coccoliths. ( i–l ) Fluorescent images of chlorophyll and accessory pigments autofluorescence. ( m–p ) Fluorescent images of dead cells stained with Sytox green. Percentages indicate the number of dead cells counted in each population. For each value n  >  300 and the standard deviation between several analyzed fields was up to 20% of the indicated value. ( q–t ) Overlay of phase contrast microscopy images (grey) with fluorescent images of TUNEL assay (green) of cultures at day 20 (see Materials and methods). ( q ) Co-culture, ( r ) Axenic algal culture, ( s ) Positive control, cells were pretreated with DNase I, ( t ) Negative control, the terminal deoxynucleotidyl transferase enzyme (TdT) was replaced with distilled water. Percentages indicate the number of positively stained cells counted in each population. For each value n  >  300 and the standard deviation between several analyzed fields was up to 25% of the indicated value. Scale bar corresponds to 1 µm in e–p and 4 µm in q–t. DOI: http://dx.doi.org/10.7554/eLife.17473.009

    Article Snippet: This section was revised and now includes the following details: “Positive controls were generated by pretreating cells with 15 U ml -1 DNase I (Thermo Fischer Scientific).

    Techniques: Microscopy, Staining, Standard Deviation, TUNEL Assay, Co-Culture Assay, Positive Control, Negative Control

    NETs contribute to the pathogenesis of myocarditis. (a) Confocal immunofluorescence images of an EMB of patient 6 suffering from PVB19-positive myocarditis (see Table 1 ). Top: Images show staining of H2A-H2B-DNA, MPO, and H3Cit as well as the merged image. Bar, 20 µm. Bottom: Magnification of a selected area of the merged confocal image (box) from the top panel. Arrows indicate NETs. Bar, 10 µm. (b and c) EAM score (b) as well as evaluation of the heart/body weight ratio (c) at day 21 after induction of EAM or sham immunization (sham; mean EAM score, 0.5 ± 0.19; mean heart/body weight ratio, 0.57 ± 0.01). Mice were treated with DNase (EAM + DNase: mean EAM score, 0.8 ± 0.19; mean heart/body weight ratio, 0.62 ± 0.005), Cl-amid (EAM + Cl-amid: mean EAM score, 0.9 ± 0.20; mean heart/body weight ratio, 0.62 ± 0.01), or vehicle as control (EAM + vehicle: mean EAM score, 1.7 ± 0.25; mean heart/body weight ratio, 0.71 ± 0.04) as indicated. n = 8 for sham group; n = 26 for EAM groups. (d) Left: Representative confocal images of cardiac sections of immunized WT mice at day 21 after induction of EAM (EAM + vehicle). Images show staining of DNA, NE, and H3Cit as well as the merged image. Bars, 20 µm. Right: Representative confocal magnified image of NETs. Images show staining of DNA, NE, and H3Cit as well as the merged image, colocalization (coloc) of DNA/NE, and DNA/H3Cit as indicated. Bars, 10 µm. (e) Representative confocal images of cardiac sections of EAM mice at day 21 after induction of EAM and blockade of MK for 21 d. Images show staining of DNA, NE, and H3Cit as well as the merged image. Bars, 20 µm. (f–h) Histological analysis of extravasated PMN into the cardiac tissue at day 21 after induction of EAM without blocking MK (EAM + vehicle) or after application of an anti-N-MK blocking Ab (EAM + anti-N-MK). Diagrams show the number of extravasated PMNs per mm 2 (f: vehicle, 768 ± 55 PMN/mm 2 ; anti-N-MK, 158 ± 86 PMN/mm 2 ), the number of NET + PMNs per mm 2 (g: vehicle, 40 ± 6 NET + PMN; anti-N-MK = 5 ± 2 NET + PMN), and the number of NET + PMNs as a percentage of all extravasated PMNs (h: vehicle, 5.1 ± 0.5 NET + PMN [%]; anti-N-MK, 0.9 ± 0.6 NET + PMN [%]). n = 6 mice. To determine P values, ANOVA on ranks with Dunn’s multiple comparisons test was performed for c and d, and an unpaired Student's t test was used for f–h. For all panels, *, P

    Journal: The Journal of Experimental Medicine

    Article Title: Midkine drives cardiac inflammation by promoting neutrophil trafficking and NETosis in myocarditis

    doi: 10.1084/jem.20181102

    Figure Lengend Snippet: NETs contribute to the pathogenesis of myocarditis. (a) Confocal immunofluorescence images of an EMB of patient 6 suffering from PVB19-positive myocarditis (see Table 1 ). Top: Images show staining of H2A-H2B-DNA, MPO, and H3Cit as well as the merged image. Bar, 20 µm. Bottom: Magnification of a selected area of the merged confocal image (box) from the top panel. Arrows indicate NETs. Bar, 10 µm. (b and c) EAM score (b) as well as evaluation of the heart/body weight ratio (c) at day 21 after induction of EAM or sham immunization (sham; mean EAM score, 0.5 ± 0.19; mean heart/body weight ratio, 0.57 ± 0.01). Mice were treated with DNase (EAM + DNase: mean EAM score, 0.8 ± 0.19; mean heart/body weight ratio, 0.62 ± 0.005), Cl-amid (EAM + Cl-amid: mean EAM score, 0.9 ± 0.20; mean heart/body weight ratio, 0.62 ± 0.01), or vehicle as control (EAM + vehicle: mean EAM score, 1.7 ± 0.25; mean heart/body weight ratio, 0.71 ± 0.04) as indicated. n = 8 for sham group; n = 26 for EAM groups. (d) Left: Representative confocal images of cardiac sections of immunized WT mice at day 21 after induction of EAM (EAM + vehicle). Images show staining of DNA, NE, and H3Cit as well as the merged image. Bars, 20 µm. Right: Representative confocal magnified image of NETs. Images show staining of DNA, NE, and H3Cit as well as the merged image, colocalization (coloc) of DNA/NE, and DNA/H3Cit as indicated. Bars, 10 µm. (e) Representative confocal images of cardiac sections of EAM mice at day 21 after induction of EAM and blockade of MK for 21 d. Images show staining of DNA, NE, and H3Cit as well as the merged image. Bars, 20 µm. (f–h) Histological analysis of extravasated PMN into the cardiac tissue at day 21 after induction of EAM without blocking MK (EAM + vehicle) or after application of an anti-N-MK blocking Ab (EAM + anti-N-MK). Diagrams show the number of extravasated PMNs per mm 2 (f: vehicle, 768 ± 55 PMN/mm 2 ; anti-N-MK, 158 ± 86 PMN/mm 2 ), the number of NET + PMNs per mm 2 (g: vehicle, 40 ± 6 NET + PMN; anti-N-MK = 5 ± 2 NET + PMN), and the number of NET + PMNs as a percentage of all extravasated PMNs (h: vehicle, 5.1 ± 0.5 NET + PMN [%]; anti-N-MK, 0.9 ± 0.6 NET + PMN [%]). n = 6 mice. To determine P values, ANOVA on ranks with Dunn’s multiple comparisons test was performed for c and d, and an unpaired Student's t test was used for f–h. For all panels, *, P

    Article Snippet: RNA was isolated using TRIzol (15596026; Thermo Fisher) according to the manufacturer’s protocol, and genomic DNA was digested using the DNase I Amplification Grade kit (18068015; Thermo Fisher).

    Techniques: Immunofluorescence, Staining, Mouse Assay, Blocking Assay

    Enzymatic resistance of phosphorothioate-modified Au-TDNNs. (A, B) Fluorescence time graph depicting terminal-modified Au-TDNN and overall-modified Au-TDNN degradation by DNase I. (C, D) Fluorescence time graph depicting terminal-modified Au-TDNN and overall-modified Au-TDNN degradation by Exo III.

    Journal: Theranostics

    Article Title: High-Discrimination Factor Nanosensor Based on Tetrahedral DNA Nanostructures and Gold Nanoparticles for Detection of MiRNA-21 in Live Cells

    doi: 10.7150/thno.23852

    Figure Lengend Snippet: Enzymatic resistance of phosphorothioate-modified Au-TDNNs. (A, B) Fluorescence time graph depicting terminal-modified Au-TDNN and overall-modified Au-TDNN degradation by DNase I. (C, D) Fluorescence time graph depicting terminal-modified Au-TDNN and overall-modified Au-TDNN degradation by Exo III.

    Article Snippet: For both human DNase I (Thermo Scientific) and exonuclease III (New England Biolabs) digestion, a common concentration of 3 U/mL was used to digest 2 nM Au-TDNNs samples in PBS.

    Techniques: Modification, Fluorescence