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tiangen biotech co dnaase i
Dnaase I, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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dnaase i - by Bioz Stars, 2020-04
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Amplification:

Article Title: Corynebacterium glutamicum Contains 3-Deoxy-d-Arabino-Heptulosonate 7-Phosphate Synthases That Display Novel Biochemical Features ▿-Arabino-Heptulosonate 7-Phosphate Synthases That Display Novel Biochemical Features ▿ †
Article Snippet: The RNA preparation was first treated with DNase I and then used as a template for reverse transcription with Moloney murine leukemia virus reverse transcriptase (TIANGEN, Beijing, China). .. The amplification of the NCgl0950 fragment from the reverse transcription PCR (RT-PCR) products (cDNAs) was conducted with the primers listed in Table .

Expressing:

Article Title: Role of metformin in inhibiting estrogen-induced proliferation and regulating ERα and ERβ expression in human endometrial cancer cells
Article Snippet: In addition, to assess the role of AMPK on ER expression, cells were treated with metformin (5 mM) with or without pre-treatment with compound C (5 µM) for 24 h. Total RNA was extracted from the cells using TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. .. The RNA samples were subjected to DNase I (TIANGEN Biotech Co., Ltd., Beijing, China) digestion to avoid possible genomic DNA contamination and were reverse transcribed with oligo-dT primers (Qiagen GmbH, Hilden, Germany) and Moloney murine leukemia virus reverse transcriptase (Promega Corporation, Madison, WI, USA).

Article Title: AmrZ Regulates Swarming Motility Through Cyclic di-GMP-Dependent Motility Inhibition and Controlling Pel Polysaccharide Production in Pseudomonas aeruginosa PA14
Article Snippet: To remove residual DNA, RNA was further treated with DNase I, repurified with an RNAclean Kit (TIANGEN), and was confirmed to be free of DNA by PCR. cDNA was synthesized with a PrimeScript RT Reagent Kit (Perfect Real Time) (TaKaRa, Liaoning, China) using random hexamer primers. qRT-PCR was performed on the Bio-Rad CFX96 Touch real-time PCR detection system using SYBR® Premix Ex Taq™ (Tli RNaseH Plus) mix (Takara). .. The mRNA levels were normalized by the geometric mean of PA14_26910 (PA2875 ) and PA14_20860 (PA3340 ) transcript levels , and relative gene expression was calculated using the 2−ΔΔCt method ( ).

Article Title: Identification and Characterization of C-type Lectins in Ostrinia furnacalis (Lepidoptera: Pyralidae)
Article Snippet: Paragraph title: Expression Profile Analysis ... One μg of RNA equally from five individual RNA samples in each stage was treated with DNase I (TIANGEN, Beijing, China) and converted into first-strand cDNA from an oligo (dT) primer following the instructions for QuantScriptRT Kit (TIANGEN, Beijing, China).

Article Title: De novo transcriptome assembly of the wild relative of tea tree (Camellia taliensis) and comparative analysis with tea transcriptome identified putative genes associated with tea quality and stress response
Article Snippet: Paragraph title: Experimental validation of candidate genes and levels of gene expression by using RT-qPCR ... After treated with DNase I (Tiangen, China), one microgram of RNA was used in reverse transcription with the SuperScript VILO cDNA Synthesis Kit (Invitrogen) according to the manufacturer’s guidelines.

Article Title: Transcriptome Analyses Reveal the Involvement of Both C and N Termini of Cryptochrome 1 in Its Regulation of Phytohormone-Responsive Gene Expression in Arabidopsis
Article Snippet: Total RNAs were isolated with RNAprep Plant kit (TIANGEN) followed by DNase I (TIANGEN) treatment. .. To validate our expression profile data, we selected genes that showed significant expression changements according to our different aim and performed real-time quantitative PCR. qRT-PCR was described previously (Zhang et al., ; He et al., ) and ACT2 was used as internal control for qRT-PCR.

Article Title: Cloning, Expression, and Characterization of Prophenoloxidases from Asian Corn Borer, Ostrinia furnacalis (Gunée)
Article Snippet: Paragraph title: 2.4. Reverse Transcriptase- (RT-) PCR Analysis of the Expression Profiles of O. furnacalis PPOs ... 1 μ g of RNA samples equally from 5 individual RNA samples in each stage was treated with DNase I (TIANGEN, Biotech (Beijing) Co., Ltd., China) and converted into first-strand cDNA from an oligo (dT) primer following the instructions for QuantScriptRT Kit (TIANGEN, Biotech (Beijing) Co., Ltd, China).

Synthesized:

Article Title: AmrZ Regulates Swarming Motility Through Cyclic di-GMP-Dependent Motility Inhibition and Controlling Pel Polysaccharide Production in Pseudomonas aeruginosa PA14
Article Snippet: .. To remove residual DNA, RNA was further treated with DNase I, repurified with an RNAclean Kit (TIANGEN), and was confirmed to be free of DNA by PCR. cDNA was synthesized with a PrimeScript RT Reagent Kit (Perfect Real Time) (TaKaRa, Liaoning, China) using random hexamer primers. qRT-PCR was performed on the Bio-Rad CFX96 Touch real-time PCR detection system using SYBR® Premix Ex Taq™ (Tli RNaseH Plus) mix (Takara). .. The mRNA levels were normalized by the geometric mean of PA14_26910 (PA2875 ) and PA14_20860 (PA3340 ) transcript levels , and relative gene expression was calculated using the 2−ΔΔCt method ( ).

Article Title: Transcriptome Analysis Provides a Preliminary Regulation Route of the Ethylene Signal Transduction Component, SlEIN2, during Tomato Ripening
Article Snippet: Unwanted genomic DNA was digested using DNase I (Tiangen Biotech Co., Beijing, China). .. Complementary DNA was then synthesized from 2 μg RNA using the TransScript ® II One-Step gDNA Removal and cDNA Synthesis SuperMix Kit (Transgen Biotech Co., LTD., Beijing, China) with oligo(dTs).

Quantitative RT-PCR:

Article Title: Role of metformin in inhibiting estrogen-induced proliferation and regulating ERα and ERβ expression in human endometrial cancer cells
Article Snippet: Paragraph title: Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) ... The RNA samples were subjected to DNase I (TIANGEN Biotech Co., Ltd., Beijing, China) digestion to avoid possible genomic DNA contamination and were reverse transcribed with oligo-dT primers (Qiagen GmbH, Hilden, Germany) and Moloney murine leukemia virus reverse transcriptase (Promega Corporation, Madison, WI, USA).

Article Title: AmrZ Regulates Swarming Motility Through Cyclic di-GMP-Dependent Motility Inhibition and Controlling Pel Polysaccharide Production in Pseudomonas aeruginosa PA14
Article Snippet: .. To remove residual DNA, RNA was further treated with DNase I, repurified with an RNAclean Kit (TIANGEN), and was confirmed to be free of DNA by PCR. cDNA was synthesized with a PrimeScript RT Reagent Kit (Perfect Real Time) (TaKaRa, Liaoning, China) using random hexamer primers. qRT-PCR was performed on the Bio-Rad CFX96 Touch real-time PCR detection system using SYBR® Premix Ex Taq™ (Tli RNaseH Plus) mix (Takara). .. The mRNA levels were normalized by the geometric mean of PA14_26910 (PA2875 ) and PA14_20860 (PA3340 ) transcript levels , and relative gene expression was calculated using the 2−ΔΔCt method ( ).

Article Title: De novo transcriptome assembly of the wild relative of tea tree (Camellia taliensis) and comparative analysis with tea transcriptome identified putative genes associated with tea quality and stress response
Article Snippet: Paragraph title: Experimental validation of candidate genes and levels of gene expression by using RT-qPCR ... After treated with DNase I (Tiangen, China), one microgram of RNA was used in reverse transcription with the SuperScript VILO cDNA Synthesis Kit (Invitrogen) according to the manufacturer’s guidelines.

Article Title: Transcriptome Analyses Reveal the Involvement of Both C and N Termini of Cryptochrome 1 in Its Regulation of Phytohormone-Responsive Gene Expression in Arabidopsis
Article Snippet: Total RNAs were isolated with RNAprep Plant kit (TIANGEN) followed by DNase I (TIANGEN) treatment. .. To validate our expression profile data, we selected genes that showed significant expression changements according to our different aim and performed real-time quantitative PCR. qRT-PCR was described previously (Zhang et al., ; He et al., ) and ACT2 was used as internal control for qRT-PCR.

SYBR Green Assay:

Article Title: Role of metformin in inhibiting estrogen-induced proliferation and regulating ERα and ERβ expression in human endometrial cancer cells
Article Snippet: The RNA samples were subjected to DNase I (TIANGEN Biotech Co., Ltd., Beijing, China) digestion to avoid possible genomic DNA contamination and were reverse transcribed with oligo-dT primers (Qiagen GmbH, Hilden, Germany) and Moloney murine leukemia virus reverse transcriptase (Promega Corporation, Madison, WI, USA). .. Reactions were performed using LightCycler 480 SYBR-Green I PCR Mastermix (Roche Diagnostics, Indianapolis, IN, USA) in a 20-µl reaction, containing 1 µl cDNA, 8.2 µl DNase/RNase-free deionized water, 10 µl SYBR-Green I Mastermix and 0.4 µl each primer (10 µM).

Random Hexamer Labeling:

Article Title: AmrZ Regulates Swarming Motility Through Cyclic di-GMP-Dependent Motility Inhibition and Controlling Pel Polysaccharide Production in Pseudomonas aeruginosa PA14
Article Snippet: .. To remove residual DNA, RNA was further treated with DNase I, repurified with an RNAclean Kit (TIANGEN), and was confirmed to be free of DNA by PCR. cDNA was synthesized with a PrimeScript RT Reagent Kit (Perfect Real Time) (TaKaRa, Liaoning, China) using random hexamer primers. qRT-PCR was performed on the Bio-Rad CFX96 Touch real-time PCR detection system using SYBR® Premix Ex Taq™ (Tli RNaseH Plus) mix (Takara). .. The mRNA levels were normalized by the geometric mean of PA14_26910 (PA2875 ) and PA14_20860 (PA3340 ) transcript levels , and relative gene expression was calculated using the 2−ΔΔCt method ( ).

Mass Spectrometry:

Article Title: Transcriptome Analyses Reveal the Involvement of Both C and N Termini of Cryptochrome 1 in Its Regulation of Phytohormone-Responsive Gene Expression in Arabidopsis
Article Snippet: Seedlings were germinated on half-strength MS plates plus 1% sucrose and placed at 4°C for 3 days and then transferred to white light for 12 h before all seedlings placed in darkness for another 4 days. .. Total RNAs were isolated with RNAprep Plant kit (TIANGEN) followed by DNase I (TIANGEN) treatment.

Modification:

Article Title: An L1 box binding protein, GbML1, interacts with GbMYB25 to control cotton fibre development
Article Snippet: Extraction of RNA and DNA RNA and DNA were extracted from different tissues of G. barbadense plants using a modified CTAB-method as described by . .. All extracted RNA was treated by DNase I and purified using the RNAprep Plant RNA Purification Kit (Tiangen) according to the manufacturer's instructions.

Article Title: De novo transcriptome assembly of the wild relative of tea tree (Camellia taliensis) and comparative analysis with tea transcriptome identified putative genes associated with tea quality and stress response
Article Snippet: Gene-specific primer pairs were designed using Primer primer 5.0 software (Premier Biosoft International), and total RNA was isolated from tender shoots, young leaves, flower bud and flower from C. taliensis using a modified CTAB method, respectively. .. After treated with DNase I (Tiangen, China), one microgram of RNA was used in reverse transcription with the SuperScript VILO cDNA Synthesis Kit (Invitrogen) according to the manufacturer’s guidelines.

Reverse Transcription Polymerase Chain Reaction:

Article Title: Identification and Characterization of C-type Lectins in Ostrinia furnacalis (Lepidoptera: Pyralidae)
Article Snippet: One μg of RNA equally from five individual RNA samples in each stage was treated with DNase I (TIANGEN, Beijing, China) and converted into first-strand cDNA from an oligo (dT) primer following the instructions for QuantScriptRT Kit (TIANGEN, Beijing, China). .. The cDNA products independently from three biological replicates were diluted 10-fold for use as template in RT-PCR experiments.

Article Title: Cloning, Expression, and Characterization of Prophenoloxidases from Asian Corn Borer, Ostrinia furnacalis (Gunée)
Article Snippet: Paragraph title: 2.4. Reverse Transcriptase- (RT-) PCR Analysis of the Expression Profiles of O. furnacalis PPOs ... 1 μ g of RNA samples equally from 5 individual RNA samples in each stage was treated with DNase I (TIANGEN, Biotech (Beijing) Co., Ltd., China) and converted into first-strand cDNA from an oligo (dT) primer following the instructions for QuantScriptRT Kit (TIANGEN, Biotech (Beijing) Co., Ltd, China).

Article Title: Corynebacterium glutamicum Contains 3-Deoxy-d-Arabino-Heptulosonate 7-Phosphate Synthases That Display Novel Biochemical Features ▿-Arabino-Heptulosonate 7-Phosphate Synthases That Display Novel Biochemical Features ▿ †
Article Snippet: Paragraph title: RNA isolation and RT-PCR. ... The RNA preparation was first treated with DNase I and then used as a template for reverse transcription with Moloney murine leukemia virus reverse transcriptase (TIANGEN, Beijing, China).

Sequencing:

Article Title: Companion cropping with potato onion enhances the disease resistance of tomato against Verticillium dahliae
Article Snippet: .. RNA-seq library preparation and sequencing Total RNA was extracted from different samples (TM1, TM2, TM3, and TC1, TC2, TC3), respectively and treated with DNase I to degrade any possible DNA contamination using RNAprep pure Plant Kit (TIANGEN, China) as described by the manufacturer. .. RNA was quantified using Agilent 2100 Bioanalyzer (Agilent Technologies, USA), the quality and integrity were assessed by NanoDrop.

Nucleic Acid Electrophoresis:

Article Title: Transcriptome Analysis Provides a Preliminary Regulation Route of the Ethylene Signal Transduction Component, SlEIN2, during Tomato Ripening
Article Snippet: Unwanted genomic DNA was digested using DNase I (Tiangen Biotech Co., Beijing, China). .. The RNA integrity estimated through gel electrophoresis showed a 28S/18S brightness ratio of approximately 2:1.

RNA Sequencing Assay:

Article Title: Companion cropping with potato onion enhances the disease resistance of tomato against Verticillium dahliae
Article Snippet: .. RNA-seq library preparation and sequencing Total RNA was extracted from different samples (TM1, TM2, TM3, and TC1, TC2, TC3), respectively and treated with DNase I to degrade any possible DNA contamination using RNAprep pure Plant Kit (TIANGEN, China) as described by the manufacturer. .. RNA was quantified using Agilent 2100 Bioanalyzer (Agilent Technologies, USA), the quality and integrity were assessed by NanoDrop.

Mutagenesis:

Article Title: Transcriptome Analyses Reveal the Involvement of Both C and N Termini of Cryptochrome 1 in Its Regulation of Phytohormone-Responsive Gene Expression in Arabidopsis
Article Snippet: Real-time quantitative PCR We used WT, cry1 mutant, CRY1-ovx, CCT1 , and CNT1 seedlings for real-time quantitative PCR. .. Total RNAs were isolated with RNAprep Plant kit (TIANGEN) followed by DNase I (TIANGEN) treatment.

Isolation:

Article Title: De novo transcriptome assembly of the wild relative of tea tree (Camellia taliensis) and comparative analysis with tea transcriptome identified putative genes associated with tea quality and stress response
Article Snippet: Gene-specific primer pairs were designed using Primer primer 5.0 software (Premier Biosoft International), and total RNA was isolated from tender shoots, young leaves, flower bud and flower from C. taliensis using a modified CTAB method, respectively. .. After treated with DNase I (Tiangen, China), one microgram of RNA was used in reverse transcription with the SuperScript VILO cDNA Synthesis Kit (Invitrogen) according to the manufacturer’s guidelines.

Article Title: Transcriptome Analysis Provides a Preliminary Regulation Route of the Ethylene Signal Transduction Component, SlEIN2, during Tomato Ripening
Article Snippet: Paragraph title: RNA isolation and real-time quantitative PCR ... Unwanted genomic DNA was digested using DNase I (Tiangen Biotech Co., Beijing, China).

Article Title: Transcriptome Analyses Reveal the Involvement of Both C and N Termini of Cryptochrome 1 in Its Regulation of Phytohormone-Responsive Gene Expression in Arabidopsis
Article Snippet: .. Total RNAs were isolated with RNAprep Plant kit (TIANGEN) followed by DNase I (TIANGEN) treatment. .. Then 500 ng sample of total RNA were used to reverse-transcribed to 10 μl cDNA using iScriptcDNA Synthesis kit (Bio-Rad).

Article Title: Corynebacterium glutamicum Contains 3-Deoxy-d-Arabino-Heptulosonate 7-Phosphate Synthases That Display Novel Biochemical Features ▿-Arabino-Heptulosonate 7-Phosphate Synthases That Display Novel Biochemical Features ▿ †
Article Snippet: Paragraph title: RNA isolation and RT-PCR. ... The RNA preparation was first treated with DNase I and then used as a template for reverse transcription with Moloney murine leukemia virus reverse transcriptase (TIANGEN, Beijing, China).

Size-exclusion Chromatography:

Article Title: Role of metformin in inhibiting estrogen-induced proliferation and regulating ERα and ERβ expression in human endometrial cancer cells
Article Snippet: The RNA samples were subjected to DNase I (TIANGEN Biotech Co., Ltd., Beijing, China) digestion to avoid possible genomic DNA contamination and were reverse transcribed with oligo-dT primers (Qiagen GmbH, Hilden, Germany) and Moloney murine leukemia virus reverse transcriptase (Promega Corporation, Madison, WI, USA). .. The cycling conditions for PCR were as follows: 95°C for 30 sec, followed by 40 cycles (two steps) of 95°C for 5 sec and 60°C for 31 sec. PCR was performed in triplicate for each sample on an ABI 7500 Real Time PCR Instrument (Applied Biosystems; Thermo Fisher Scientific, Inc.) to detect the fluorescent signals.

Purification:

Article Title: An L1 box binding protein, GbML1, interacts with GbMYB25 to control cotton fibre development
Article Snippet: .. All extracted RNA was treated by DNase I and purified using the RNAprep Plant RNA Purification Kit (Tiangen) according to the manufacturer's instructions. .. The concentration of the purified RNA and DNA was quantified by a nucleic acid analyser (DU-640, Beckman).

Polymerase Chain Reaction:

Article Title: Role of metformin in inhibiting estrogen-induced proliferation and regulating ERα and ERβ expression in human endometrial cancer cells
Article Snippet: Paragraph title: Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) ... The RNA samples were subjected to DNase I (TIANGEN Biotech Co., Ltd., Beijing, China) digestion to avoid possible genomic DNA contamination and were reverse transcribed with oligo-dT primers (Qiagen GmbH, Hilden, Germany) and Moloney murine leukemia virus reverse transcriptase (Promega Corporation, Madison, WI, USA).

Article Title: AmrZ Regulates Swarming Motility Through Cyclic di-GMP-Dependent Motility Inhibition and Controlling Pel Polysaccharide Production in Pseudomonas aeruginosa PA14
Article Snippet: .. To remove residual DNA, RNA was further treated with DNase I, repurified with an RNAclean Kit (TIANGEN), and was confirmed to be free of DNA by PCR. cDNA was synthesized with a PrimeScript RT Reagent Kit (Perfect Real Time) (TaKaRa, Liaoning, China) using random hexamer primers. qRT-PCR was performed on the Bio-Rad CFX96 Touch real-time PCR detection system using SYBR® Premix Ex Taq™ (Tli RNaseH Plus) mix (Takara). .. The mRNA levels were normalized by the geometric mean of PA14_26910 (PA2875 ) and PA14_20860 (PA3340 ) transcript levels , and relative gene expression was calculated using the 2−ΔΔCt method ( ).

Article Title: Identification and Characterization of C-type Lectins in Ostrinia furnacalis (Lepidoptera: Pyralidae)
Article Snippet: One μg of RNA equally from five individual RNA samples in each stage was treated with DNase I (TIANGEN, Beijing, China) and converted into first-strand cDNA from an oligo (dT) primer following the instructions for QuantScriptRT Kit (TIANGEN, Beijing, China). .. O. furnacalis ribosomal protein L8 ( rpL8 ) was used as an internal standard to adjust the template amounts in a preliminary PCR experiments.

Article Title: De novo transcriptome assembly of the wild relative of tea tree (Camellia taliensis) and comparative analysis with tea transcriptome identified putative genes associated with tea quality and stress response
Article Snippet: After treated with DNase I (Tiangen, China), one microgram of RNA was used in reverse transcription with the SuperScript VILO cDNA Synthesis Kit (Invitrogen) according to the manufacturer’s guidelines. .. The standard curve for each gene was conducted in several dilutions of cDNA, then real-time qPCR was performed using Multicolor Real-Time PCR Detection System (Bio-Rad) with conditions for all reactions were 95°C for 30s, 40 cycles of 95°C for 15 s, followed by 60°C for 30 s, and 72°C for 20 s. Melting curve and agarose gel electrophoresis analysis were performed to confirm the PCR specificity.

Article Title: Cloning, Expression, and Characterization of Prophenoloxidases from Asian Corn Borer, Ostrinia furnacalis (Gunée)
Article Snippet: 1 μ g of RNA samples equally from 5 individual RNA samples in each stage was treated with DNase I (TIANGEN, Biotech (Beijing) Co., Ltd., China) and converted into first-strand cDNA from an oligo (dT) primer following the instructions for QuantScriptRT Kit (TIANGEN, Biotech (Beijing) Co., Ltd, China). .. O. furnacalis ribosomal protein L8 (rpL8 ) was used as an internal standard to adjust the template amounts in preliminary PCR experiments.

Article Title: Corynebacterium glutamicum Contains 3-Deoxy-d-Arabino-Heptulosonate 7-Phosphate Synthases That Display Novel Biochemical Features ▿-Arabino-Heptulosonate 7-Phosphate Synthases That Display Novel Biochemical Features ▿ †
Article Snippet: The RNA preparation was first treated with DNase I and then used as a template for reverse transcription with Moloney murine leukemia virus reverse transcriptase (TIANGEN, Beijing, China). .. The amplification of the NCgl0950 fragment from the reverse transcription PCR (RT-PCR) products (cDNAs) was conducted with the primers listed in Table .

Software:

Article Title: De novo transcriptome assembly of the wild relative of tea tree (Camellia taliensis) and comparative analysis with tea transcriptome identified putative genes associated with tea quality and stress response
Article Snippet: Gene-specific primer pairs were designed using Primer primer 5.0 software (Premier Biosoft International), and total RNA was isolated from tender shoots, young leaves, flower bud and flower from C. taliensis using a modified CTAB method, respectively. .. After treated with DNase I (Tiangen, China), one microgram of RNA was used in reverse transcription with the SuperScript VILO cDNA Synthesis Kit (Invitrogen) according to the manufacturer’s guidelines.

Real-time Polymerase Chain Reaction:

Article Title: Role of metformin in inhibiting estrogen-induced proliferation and regulating ERα and ERβ expression in human endometrial cancer cells
Article Snippet: The RNA samples were subjected to DNase I (TIANGEN Biotech Co., Ltd., Beijing, China) digestion to avoid possible genomic DNA contamination and were reverse transcribed with oligo-dT primers (Qiagen GmbH, Hilden, Germany) and Moloney murine leukemia virus reverse transcriptase (Promega Corporation, Madison, WI, USA). .. The cycling conditions for PCR were as follows: 95°C for 30 sec, followed by 40 cycles (two steps) of 95°C for 5 sec and 60°C for 31 sec. PCR was performed in triplicate for each sample on an ABI 7500 Real Time PCR Instrument (Applied Biosystems; Thermo Fisher Scientific, Inc.) to detect the fluorescent signals.

Article Title: AmrZ Regulates Swarming Motility Through Cyclic di-GMP-Dependent Motility Inhibition and Controlling Pel Polysaccharide Production in Pseudomonas aeruginosa PA14
Article Snippet: .. To remove residual DNA, RNA was further treated with DNase I, repurified with an RNAclean Kit (TIANGEN), and was confirmed to be free of DNA by PCR. cDNA was synthesized with a PrimeScript RT Reagent Kit (Perfect Real Time) (TaKaRa, Liaoning, China) using random hexamer primers. qRT-PCR was performed on the Bio-Rad CFX96 Touch real-time PCR detection system using SYBR® Premix Ex Taq™ (Tli RNaseH Plus) mix (Takara). .. The mRNA levels were normalized by the geometric mean of PA14_26910 (PA2875 ) and PA14_20860 (PA3340 ) transcript levels , and relative gene expression was calculated using the 2−ΔΔCt method ( ).

Article Title: De novo transcriptome assembly of the wild relative of tea tree (Camellia taliensis) and comparative analysis with tea transcriptome identified putative genes associated with tea quality and stress response
Article Snippet: After treated with DNase I (Tiangen, China), one microgram of RNA was used in reverse transcription with the SuperScript VILO cDNA Synthesis Kit (Invitrogen) according to the manufacturer’s guidelines. .. The standard curve for each gene was conducted in several dilutions of cDNA, then real-time qPCR was performed using Multicolor Real-Time PCR Detection System (Bio-Rad) with conditions for all reactions were 95°C for 30s, 40 cycles of 95°C for 15 s, followed by 60°C for 30 s, and 72°C for 20 s. Melting curve and agarose gel electrophoresis analysis were performed to confirm the PCR specificity.

Article Title: Transcriptome Analysis Provides a Preliminary Regulation Route of the Ethylene Signal Transduction Component, SlEIN2, during Tomato Ripening
Article Snippet: Paragraph title: RNA isolation and real-time quantitative PCR ... Unwanted genomic DNA was digested using DNase I (Tiangen Biotech Co., Beijing, China).

Article Title: Transcriptome Analyses Reveal the Involvement of Both C and N Termini of Cryptochrome 1 in Its Regulation of Phytohormone-Responsive Gene Expression in Arabidopsis
Article Snippet: Paragraph title: Real-time quantitative PCR ... Total RNAs were isolated with RNAprep Plant kit (TIANGEN) followed by DNase I (TIANGEN) treatment.

Agarose Gel Electrophoresis:

Article Title: An L1 box binding protein, GbML1, interacts with GbMYB25 to control cotton fibre development
Article Snippet: All extracted RNA was treated by DNase I and purified using the RNAprep Plant RNA Purification Kit (Tiangen) according to the manufacturer's instructions. .. The quality and concentration of the purified RNA were further checked by RNA denaturing MOPS agarose gel.

Article Title: De novo transcriptome assembly of the wild relative of tea tree (Camellia taliensis) and comparative analysis with tea transcriptome identified putative genes associated with tea quality and stress response
Article Snippet: After treated with DNase I (Tiangen, China), one microgram of RNA was used in reverse transcription with the SuperScript VILO cDNA Synthesis Kit (Invitrogen) according to the manufacturer’s guidelines. .. The standard curve for each gene was conducted in several dilutions of cDNA, then real-time qPCR was performed using Multicolor Real-Time PCR Detection System (Bio-Rad) with conditions for all reactions were 95°C for 30s, 40 cycles of 95°C for 15 s, followed by 60°C for 30 s, and 72°C for 20 s. Melting curve and agarose gel electrophoresis analysis were performed to confirm the PCR specificity.

Incubation:

Article Title: AmrZ Regulates Swarming Motility Through Cyclic di-GMP-Dependent Motility Inhibition and Controlling Pel Polysaccharide Production in Pseudomonas aeruginosa PA14
Article Snippet: Biofilms were grown directly on 6-well polystyrene microplates (Costar #3506) in liquid swarming medium and incubated as a static culture for 6 and 24 h at 37°C ( ; ; ). .. To remove residual DNA, RNA was further treated with DNase I, repurified with an RNAclean Kit (TIANGEN), and was confirmed to be free of DNA by PCR. cDNA was synthesized with a PrimeScript RT Reagent Kit (Perfect Real Time) (TaKaRa, Liaoning, China) using random hexamer primers. qRT-PCR was performed on the Bio-Rad CFX96 Touch real-time PCR detection system using SYBR® Premix Ex Taq™ (Tli RNaseH Plus) mix (Takara).

Spectrophotometry:

Article Title: Transcriptome Analysis Provides a Preliminary Regulation Route of the Ethylene Signal Transduction Component, SlEIN2, during Tomato Ripening
Article Snippet: Unwanted genomic DNA was digested using DNase I (Tiangen Biotech Co., Beijing, China). .. The concentration and purity of RNA were measured in a NAS-99 spectrophotometer (ATCGene Inc., New Jersey, United States).

Concentration Assay:

Article Title: An L1 box binding protein, GbML1, interacts with GbMYB25 to control cotton fibre development
Article Snippet: All extracted RNA was treated by DNase I and purified using the RNAprep Plant RNA Purification Kit (Tiangen) according to the manufacturer's instructions. .. The concentration of the purified RNA and DNA was quantified by a nucleic acid analyser (DU-640, Beckman).

Article Title: Role of metformin in inhibiting estrogen-induced proliferation and regulating ERα and ERβ expression in human endometrial cancer cells
Article Snippet: Ishikawa and HEC-1-A cells were plated at a concentration of 105 cells/well in 6-well plates for 24 h at 37°C and subsequently treated with metformin (0, 1, 5 and 15 mM) in McCoy's 5tA or RPMI-1640 medium containing 5% FBS, respectively, for 24 h at 37°C. .. The RNA samples were subjected to DNase I (TIANGEN Biotech Co., Ltd., Beijing, China) digestion to avoid possible genomic DNA contamination and were reverse transcribed with oligo-dT primers (Qiagen GmbH, Hilden, Germany) and Moloney murine leukemia virus reverse transcriptase (Promega Corporation, Madison, WI, USA).

Article Title: Transcriptome Analysis Provides a Preliminary Regulation Route of the Ethylene Signal Transduction Component, SlEIN2, during Tomato Ripening
Article Snippet: Unwanted genomic DNA was digested using DNase I (Tiangen Biotech Co., Beijing, China). .. The concentration and purity of RNA were measured in a NAS-99 spectrophotometer (ATCGene Inc., New Jersey, United States).

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    tiangen biotech co t7 endonuclease i assay dna extraction
    CRISPR/Cas9 induces targeted <t>DNA</t> cleavage in chicken cells. a Co-transfection of U6-gRNA-pHEf1A-Cas9-mKate and EYFP-Truncated plasmids in chicken DF-1 (48 h); b Flow cytometry analysis of EYFP percentage in co-transfected DF-1 cells in ( a ); c Experimental scheme for targeted DAZL gene knock out in DF-1 cells; d T7 endonuclease I assay of the DAZL gene mutation in chicken DF-1 cells; e Sequencing analysis of targeted mutation in the DAZL gene
    T7 Endonuclease I Assay Dna Extraction, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t7 endonuclease i assay dna extraction/product/tiangen biotech co
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t7 endonuclease i assay dna extraction - by Bioz Stars, 2020-04
    94/100 stars
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    97
    tiangen biotech co dnase i
    CRISPR/Cas9 induces targeted <t>DNA</t> cleavage in chicken cells. a Co-transfection of U6-gRNA-pHEf1A-Cas9-mKate and EYFP-Truncated plasmids in chicken DF-1 (48 h); b Flow cytometry analysis of EYFP percentage in co-transfected DF-1 cells in ( a ); c Experimental scheme for targeted DAZL gene knock out in DF-1 cells; d T7 endonuclease I assay of the DAZL gene mutation in chicken DF-1 cells; e Sequencing analysis of targeted mutation in the DAZL gene
    Dnase I, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 97/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase i/product/tiangen biotech co
    Average 97 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    dnase i - by Bioz Stars, 2020-04
    97/100 stars
      Buy from Supplier

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    tiangen biotech co on column dnase i digestion
    CRISPR/Cas9 induces targeted <t>DNA</t> cleavage in chicken cells. a Co-transfection of U6-gRNA-pHEf1A-Cas9-mKate and EYFP-Truncated plasmids in chicken DF-1 (48 h); b Flow cytometry analysis of EYFP percentage in co-transfected DF-1 cells in ( a ); c Experimental scheme for targeted DAZL gene knock out in DF-1 cells; d T7 endonuclease I assay of the DAZL gene mutation in chicken DF-1 cells; e Sequencing analysis of targeted mutation in the DAZL gene
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    CRISPR/Cas9 induces targeted DNA cleavage in chicken cells. a Co-transfection of U6-gRNA-pHEf1A-Cas9-mKate and EYFP-Truncated plasmids in chicken DF-1 (48 h); b Flow cytometry analysis of EYFP percentage in co-transfected DF-1 cells in ( a ); c Experimental scheme for targeted DAZL gene knock out in DF-1 cells; d T7 endonuclease I assay of the DAZL gene mutation in chicken DF-1 cells; e Sequencing analysis of targeted mutation in the DAZL gene

    Journal: Journal of Biological Engineering

    Article Title: HMEJ-mediated efficient site-specific gene integration in chicken cells

    doi: 10.1186/s13036-019-0217-9

    Figure Lengend Snippet: CRISPR/Cas9 induces targeted DNA cleavage in chicken cells. a Co-transfection of U6-gRNA-pHEf1A-Cas9-mKate and EYFP-Truncated plasmids in chicken DF-1 (48 h); b Flow cytometry analysis of EYFP percentage in co-transfected DF-1 cells in ( a ); c Experimental scheme for targeted DAZL gene knock out in DF-1 cells; d T7 endonuclease I assay of the DAZL gene mutation in chicken DF-1 cells; e Sequencing analysis of targeted mutation in the DAZL gene

    Article Snippet: T7 endonuclease I assay DNA extraction was performed using a DNA extraction kit (Tiangen Biotech, Beijing, China).

    Techniques: CRISPR, Cotransfection, Flow Cytometry, Cytometry, Transfection, Knock-Out, T7EI Assay, Mutagenesis, Sequencing