Journal: EMBO Reports
Article Title: Enzymatic degradation of RNA causes widespread protein aggregation in cell and tissue lysates
Figure Lengend Snippet: Enzymatic degradation of RNA causes protein aggregation Diagram showing the experimental design. SDS–PAGE analysis of soluble (Supernatant) and insoluble proteins (Pellet) from human neurons after treatment for one hour with a mixture of RNase A and RNase T1 (A/T1), or vehicle (Ve‐). Protein aggregation (Pellet) after incubation with different ribonucleases or DNase I in the presence of either EDTA or Mg 2+ . Ribonucleases used were RNase A (A), RNase T1 (T1), a mixture of RNase A and RNase T1 (A/T1), RNase 1f (1f) and RNase V1 (V1).
Article Snippet: RNA was dissolved in 30 μg 1× DNAs I buffer and treated with 1 μl DNAase I (2222, Thermo Fisher Scientific) for 30 min at 37°C.
Techniques: SDS Page, Incubation