dnaase  (Thermo Fisher)


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    Structured Review

    Thermo Fisher dnaase
    Dnaase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnaase/product/Thermo Fisher
    Average 97 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    dnaase - by Bioz Stars, 2022-08
    97/100 stars

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    Thermo Fisher dnaase i
    Enzymatic degradation of  RNA  causes protein aggregation Diagram showing the experimental design. SDS–PAGE analysis of soluble (Supernatant) and insoluble proteins (Pellet) from human neurons after treatment for one hour with a mixture of RNase A and RNase T1 (A/T1), or vehicle (Ve‐). Protein aggregation (Pellet) after incubation with different ribonucleases or DNase I in the presence of either EDTA or Mg 2+ . Ribonucleases used were RNase A (A), RNase T1 (T1), a mixture of RNase A and RNase T1 (A/T1), RNase 1f (1f) and RNase V1 (V1).
    Dnaase I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnaase i/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dnaase i - by Bioz Stars, 2022-08
    97/100 stars
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    Enzymatic degradation of  RNA  causes protein aggregation Diagram showing the experimental design. SDS–PAGE analysis of soluble (Supernatant) and insoluble proteins (Pellet) from human neurons after treatment for one hour with a mixture of RNase A and RNase T1 (A/T1), or vehicle (Ve‐). Protein aggregation (Pellet) after incubation with different ribonucleases or DNase I in the presence of either EDTA or Mg 2+ . Ribonucleases used were RNase A (A), RNase T1 (T1), a mixture of RNase A and RNase T1 (A/T1), RNase 1f (1f) and RNase V1 (V1).

    Journal: EMBO Reports

    Article Title: Enzymatic degradation of RNA causes widespread protein aggregation in cell and tissue lysates

    doi: 10.15252/embr.201949585

    Figure Lengend Snippet: Enzymatic degradation of RNA causes protein aggregation Diagram showing the experimental design. SDS–PAGE analysis of soluble (Supernatant) and insoluble proteins (Pellet) from human neurons after treatment for one hour with a mixture of RNase A and RNase T1 (A/T1), or vehicle (Ve‐). Protein aggregation (Pellet) after incubation with different ribonucleases or DNase I in the presence of either EDTA or Mg 2+ . Ribonucleases used were RNase A (A), RNase T1 (T1), a mixture of RNase A and RNase T1 (A/T1), RNase 1f (1f) and RNase V1 (V1).

    Article Snippet: RNA was dissolved in 30 μg 1× DNAs I buffer and treated with 1 μl DNAase I (2222, Thermo Fisher Scientific) for 30 min at 37°C.

    Techniques: SDS Page, Incubation

    Enzymatic degradation of RNA causes protein aggregation. a  Diagram showing the experimental design.  b  SDS-PAGE analysis of soluble (Supernatant) and insoluble proteins (Pellet) from human neurons after treatment with a mixture of RNase A and RNase T1 (A/T1), or vehicle (Ve-).  c  Protein aggregation (Pellet) after incubation with different ribonucleases or DNase I in the presence of either EDTA or Mg 2+ . Ribonucleases used were RNase A (A), RNase T1 (T1), a mixture of RNase A and RNase T1 (A/T1), RNase 1f (1f), and RNase V1 (V1),

    Journal: bioRxiv

    Article Title: Enzymatic degradation of RNA causes widespread protein aggregation in cell and tissue lysates

    doi: 10.1101/841577

    Figure Lengend Snippet: Enzymatic degradation of RNA causes protein aggregation. a Diagram showing the experimental design. b SDS-PAGE analysis of soluble (Supernatant) and insoluble proteins (Pellet) from human neurons after treatment with a mixture of RNase A and RNase T1 (A/T1), or vehicle (Ve-). c Protein aggregation (Pellet) after incubation with different ribonucleases or DNase I in the presence of either EDTA or Mg 2+ . Ribonucleases used were RNase A (A), RNase T1 (T1), a mixture of RNase A and RNase T1 (A/T1), RNase 1f (1f), and RNase V1 (V1),

    Article Snippet: RNA were dissolved in 30 μg 1x DNAs I buffer and treated with 1 μl DNAase I (2222, Thermo Fisher Scientific) for 30 min at 37°C.

    Techniques: SDS Page, Incubation