Structured Review

Millipore dnaase
Optimized decellularization method preserved ECM components H E, Alcian blue, collagen type II, AGN and collagen type I staining of normal A1. -2, B1. -2, C1. -2, D1. -2, E1. -2 and optimized method A3. -4, B3. -4, C3. -4, D3. -4, E3. -4. The content of <t>DNA</t> F. , HYP G. and water H. revealed the excellent biological characteristics of optimized method group ( n = 5 per study group). Optimized method includes 2% Triton X-100 for 24 h, 1% SDS for 24 h and 200 U/ml <t>DNAase</t> for 12 h. Abbreviations: IVD = intervertebral disc, AF = annulus fibrous, NP = nucleus pulposus, HYP = hydroxyproline. A-E: Scale bar 100μm.
Dnaase, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dnaase/product/Millipore
Average 97 stars, based on 5 article reviews
Price from $9.99 to $1999.99
dnaase - by Bioz Stars, 2022-09
97/100 stars

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1) Product Images from "Decellularized allogeneic intervertebral disc: natural biomaterials for regenerating disc degeneration"

Article Title: Decellularized allogeneic intervertebral disc: natural biomaterials for regenerating disc degeneration

Journal: Oncotarget

doi: 10.18632/oncotarget.7735

Optimized decellularization method preserved ECM components H E, Alcian blue, collagen type II, AGN and collagen type I staining of normal A1. -2, B1. -2, C1. -2, D1. -2, E1. -2 and optimized method A3. -4, B3. -4, C3. -4, D3. -4, E3. -4. The content of DNA F. , HYP G. and water H. revealed the excellent biological characteristics of optimized method group ( n = 5 per study group). Optimized method includes 2% Triton X-100 for 24 h, 1% SDS for 24 h and 200 U/ml DNAase for 12 h. Abbreviations: IVD = intervertebral disc, AF = annulus fibrous, NP = nucleus pulposus, HYP = hydroxyproline. A-E: Scale bar 100μm.
Figure Legend Snippet: Optimized decellularization method preserved ECM components H E, Alcian blue, collagen type II, AGN and collagen type I staining of normal A1. -2, B1. -2, C1. -2, D1. -2, E1. -2 and optimized method A3. -4, B3. -4, C3. -4, D3. -4, E3. -4. The content of DNA F. , HYP G. and water H. revealed the excellent biological characteristics of optimized method group ( n = 5 per study group). Optimized method includes 2% Triton X-100 for 24 h, 1% SDS for 24 h and 200 U/ml DNAase for 12 h. Abbreviations: IVD = intervertebral disc, AF = annulus fibrous, NP = nucleus pulposus, HYP = hydroxyproline. A-E: Scale bar 100μm.

Techniques Used: Staining

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    Millipore dnaase i
    CLEC5A is critical for restriction of bacterial spreading in vivo. a , b Wild type ( WT ), <t>WT/DNase-I</t> (4 KU per mouse) and mutant mice were injected with the live luminescent L. monocytogenes ( Xen32 ) (3 × 10 8 CFUs per mouse) intravenously, and luminescence intensity in liver was determined at 1 h, 2 h, and 4 h post- infection (10 mice per group) by IVIS system (Xenogen). Bacterial loads in liver c and blood d were determined 4 h post-infection. The data were collected and expressed as mean ± s.e.m. from four independent experiments ( n = 20). One-way ANOVA test performed. * P
    Dnaase I, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnaase i/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dnaase i - by Bioz Stars, 2022-09
    99/100 stars
      Buy from Supplier

    97
    Millipore dnaase
    Optimized decellularization method preserved ECM components H E, Alcian blue, collagen type II, AGN and collagen type I staining of normal A1. -2, B1. -2, C1. -2, D1. -2, E1. -2 and optimized method A3. -4, B3. -4, C3. -4, D3. -4, E3. -4. The content of <t>DNA</t> F. , HYP G. and water H. revealed the excellent biological characteristics of optimized method group ( n = 5 per study group). Optimized method includes 2% Triton X-100 for 24 h, 1% SDS for 24 h and 200 U/ml <t>DNAase</t> for 12 h. Abbreviations: IVD = intervertebral disc, AF = annulus fibrous, NP = nucleus pulposus, HYP = hydroxyproline. A-E: Scale bar 100μm.
    Dnaase, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnaase/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dnaase - by Bioz Stars, 2022-09
    97/100 stars
      Buy from Supplier

    97
    Millipore trypsin dnaase i
    Optimized decellularization method preserved ECM components H E, Alcian blue, collagen type II, AGN and collagen type I staining of normal A1. -2, B1. -2, C1. -2, D1. -2, E1. -2 and optimized method A3. -4, B3. -4, C3. -4, D3. -4, E3. -4. The content of <t>DNA</t> F. , HYP G. and water H. revealed the excellent biological characteristics of optimized method group ( n = 5 per study group). Optimized method includes 2% Triton X-100 for 24 h, 1% SDS for 24 h and 200 U/ml <t>DNAase</t> for 12 h. Abbreviations: IVD = intervertebral disc, AF = annulus fibrous, NP = nucleus pulposus, HYP = hydroxyproline. A-E: Scale bar 100μm.
    Trypsin Dnaase I, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trypsin dnaase i - by Bioz Stars, 2022-09
    97/100 stars
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    CLEC5A is critical for restriction of bacterial spreading in vivo. a , b Wild type ( WT ), WT/DNase-I (4 KU per mouse) and mutant mice were injected with the live luminescent L. monocytogenes ( Xen32 ) (3 × 10 8 CFUs per mouse) intravenously, and luminescence intensity in liver was determined at 1 h, 2 h, and 4 h post- infection (10 mice per group) by IVIS system (Xenogen). Bacterial loads in liver c and blood d were determined 4 h post-infection. The data were collected and expressed as mean ± s.e.m. from four independent experiments ( n = 20). One-way ANOVA test performed. * P

    Journal: Nature Communications

    Article Title: CLEC5A is a critical receptor in innate immunity against Listeria infection

    doi: 10.1038/s41467-017-00356-3

    Figure Lengend Snippet: CLEC5A is critical for restriction of bacterial spreading in vivo. a , b Wild type ( WT ), WT/DNase-I (4 KU per mouse) and mutant mice were injected with the live luminescent L. monocytogenes ( Xen32 ) (3 × 10 8 CFUs per mouse) intravenously, and luminescence intensity in liver was determined at 1 h, 2 h, and 4 h post- infection (10 mice per group) by IVIS system (Xenogen). Bacterial loads in liver c and blood d were determined 4 h post-infection. The data were collected and expressed as mean ± s.e.m. from four independent experiments ( n = 20). One-way ANOVA test performed. * P

    Article Snippet: The livers were collected and minced into pieces followed by being digested with the buffer containing 1 mg/ml collagenase type IV (Sigma C5138) and 300 U/ml DNAase I (Sigma D5025) at 37 °C for 30 min. Liver homogenates were passed through cell strainer (40 μm), and lysed with RBC lysis buffer to remove the remaining red blood cells.

    Techniques: In Vivo, Mutagenesis, Mouse Assay, Injection, Infection

    Optimized decellularization method preserved ECM components H E, Alcian blue, collagen type II, AGN and collagen type I staining of normal A1. -2, B1. -2, C1. -2, D1. -2, E1. -2 and optimized method A3. -4, B3. -4, C3. -4, D3. -4, E3. -4. The content of DNA F. , HYP G. and water H. revealed the excellent biological characteristics of optimized method group ( n = 5 per study group). Optimized method includes 2% Triton X-100 for 24 h, 1% SDS for 24 h and 200 U/ml DNAase for 12 h. Abbreviations: IVD = intervertebral disc, AF = annulus fibrous, NP = nucleus pulposus, HYP = hydroxyproline. A-E: Scale bar 100μm.

    Journal: Oncotarget

    Article Title: Decellularized allogeneic intervertebral disc: natural biomaterials for regenerating disc degeneration

    doi: 10.18632/oncotarget.7735

    Figure Lengend Snippet: Optimized decellularization method preserved ECM components H E, Alcian blue, collagen type II, AGN and collagen type I staining of normal A1. -2, B1. -2, C1. -2, D1. -2, E1. -2 and optimized method A3. -4, B3. -4, C3. -4, D3. -4, E3. -4. The content of DNA F. , HYP G. and water H. revealed the excellent biological characteristics of optimized method group ( n = 5 per study group). Optimized method includes 2% Triton X-100 for 24 h, 1% SDS for 24 h and 200 U/ml DNAase for 12 h. Abbreviations: IVD = intervertebral disc, AF = annulus fibrous, NP = nucleus pulposus, HYP = hydroxyproline. A-E: Scale bar 100μm.

    Article Snippet: To minimize the DNA content in the ECM, 200 U/ml DNAase (Sigma) in PBS was applied with shaking at 100 rpm and at 37°C for 12 h. Finally, the IVDs were rinsed with PBS for 6 h to remove the residue reagents effectively.

    Techniques: Staining