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Boehringer Ingelheim dnaase i
Dnaase I, supplied by Boehringer Ingelheim, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dnaase i/product/Boehringer Ingelheim
Average 84 stars, based on 1 article reviews
Price from $9.99 to $1999.99
dnaase i - by Bioz Stars, 2020-04
84/100 stars

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Related Articles

Spectrophotometry:

Article Title: Loss of Aip1 reveals a role in maintaining the actin monomer pool and an in vivo oligomer assembly pathway
Article Snippet: Extracts were used immediately and were not allowed to remain on ice for > 1 h. 200 µl of 100 nM DNase I (Boehringer Ingelheim) was added to 800 µl of 25 µg/ml herring sperm DNA. .. A260 values were measured for 1 min immediately after mixing using a spectrophotometer (Thermo Fisher Scientific).

Flow Cytometry:

Article Title: Cigarette smoke enhances Th-2 driven airway inflammation and delays inhalational tolerance
Article Snippet: Paragraph title: Tissue processing and preparation of lung single-cell suspension for flow cytometric analysis ... The right lung was thoroughly minced and incubated in digestion medium at 37°C containing DNase I (grade II from bovine pancreas; Boehringer Ingelheim, Germany) and collagenase type II (Worthington Biochemical Corp., Lakewood, NJ).

Isolation:

Article Title: Regulation of galectin-3 function in mucosal fibroblasts: potential role in mucosal inflammation
Article Snippet: Paragraph title: Isolation and culture of CLPF ... The remaining tissue was rinsed and digested for 30 min at 37°C with 1 mg/ml collagenase 1 (Sigma), 0·3 mg/ml DNase I (Boehringer Ingelheim, Ingelheim, Germany) and 2 mg/ml hyaluronidase (Sigma) in PBS (G ibco , Karlsruhe, Germany).

Article Title: Soluble galectin-3 is a strong, colonic epithelial-cell-derived, lamina propria fibroblast-stimulating factor
Article Snippet: Paragraph title: Isolation and culture of CLPFs ... The remaining tissue was rinsed and digested for 30 min at 37°C with 1 mg/ml collagenase 1 (Sigma), 0.3 mg/ml DNase I (Boehringer Ingelheim, Ingelheim, Germany) and 2 mg/ml hyaluronidase (Sigma) in phosphate‐buffered saline (PBS; Gibco, Karlsruhe, Germany).

Article Title: Characterization and Quantification of Innate Lymphoid Cell Subsets in Human Lung
Article Snippet: The lung tissue was cut into fine pieces and digested for 45 minutes at 37°C in digestion medium (Roswell Park Memorial Institute medium (RPMI) 1640 supplemented with 5% fetal calf serum (FCS), 2 mM L-glutamine, 0.05 mM 2-mercaptomethanol (all Invitrogen), 100 U/ml penicillin, 100 mg/ml streptomycin (Sigma-Aldrich), 1 mg/ml collagenase type 2 (Worthington Biochemical), and 0.02 mg/ml DNase I (grade II from bovine pancreas; Boehringer Ingelheim)). .. Next, cells were filtered through a 40-μm cell strainer and mononuclear cells were isolated with Ficoll-PaqueTM plus (GE Healthcare).

Article Title: Selective Over-Expression of Endothelin-1 in Endothelial Cells Exacerbates Inner Retinal Edema and Neuronal Death in Ischemic Retina
Article Snippet: Retinal tissues were homogenized in 1 ml ice-cold TRI REAGENT® (Molecular Research Center, Cleveland, Ohio) and the total RNA from retina was isolated according to manufacturer's protocol. .. 1.5 µg of total RNA was treated with DNase I (Boehringer, Ingelheim, Germany) and reverse transcription was performed with Oligo(dT)18, Superscript™ II RNase H- reverse transcriptase (Invitrogen, Carlsbad, CA), DTT and dNTP mixture.

Dissection:

Article Title: Spatiotemporally separated antigen uptake by alveolar dendritic cells and airway presentation to T cells in the lung
Article Snippet: Paragraph title: Lung and trachea dissection and dissociation. ... Lung lobes and trachea were separated and placed into dissociation medium composed of RPMI with 10% FCS, 400 U/ml collagenase D (Roche), and 0.25 mg/ml DNase I (Boehringer Ingelheim).

Incubation:

Article Title: Cigarette smoke enhances Th-2 driven airway inflammation and delays inhalational tolerance
Article Snippet: .. The right lung was thoroughly minced and incubated in digestion medium at 37°C containing DNase I (grade II from bovine pancreas; Boehringer Ingelheim, Germany) and collagenase type II (Worthington Biochemical Corp., Lakewood, NJ). ..

Cell Culture:

Article Title: Regulation of galectin-3 function in mucosal fibroblasts: potential role in mucosal inflammation
Article Snippet: Human CLPF were isolated and cultured as described previously [ ]. .. The remaining tissue was rinsed and digested for 30 min at 37°C with 1 mg/ml collagenase 1 (Sigma), 0·3 mg/ml DNase I (Boehringer Ingelheim, Ingelheim, Germany) and 2 mg/ml hyaluronidase (Sigma) in PBS (G ibco , Karlsruhe, Germany).

Article Title: Soluble galectin-3 is a strong, colonic epithelial-cell-derived, lamina propria fibroblast-stimulating factor
Article Snippet: Human CLPFs were isolated and cultured as described previously. .. The remaining tissue was rinsed and digested for 30 min at 37°C with 1 mg/ml collagenase 1 (Sigma), 0.3 mg/ml DNase I (Boehringer Ingelheim, Ingelheim, Germany) and 2 mg/ml hyaluronidase (Sigma) in phosphate‐buffered saline (PBS; Gibco, Karlsruhe, Germany).

Mouse Assay:

Article Title: Spatiotemporally separated antigen uptake by alveolar dendritic cells and airway presentation to T cells in the lung
Article Snippet: Mice were given a lethal dose of Avertin and exsanguinated from the descending aorta before lung dissection. .. Lung lobes and trachea were separated and placed into dissociation medium composed of RPMI with 10% FCS, 400 U/ml collagenase D (Roche), and 0.25 mg/ml DNase I (Boehringer Ingelheim).

Article Title: Selective Over-Expression of Endothelin-1 in Endothelial Cells Exacerbates Inner Retinal Edema and Neuronal Death in Ischemic Retina
Article Snippet: Semi-quantitative reverse transcriptase-PCR (rt-PCR) of ET-1 gene Mice at the age of 6 to 8 weeks were sacrificed, their eyes were cut underneath the optic nerve and the retinae were dissected under dissecting microscope (Stemi SV6; Carl Zeiss, Thornwood, NY), frozen in liquid nitrogen and stored in a −80°C. .. 1.5 µg of total RNA was treated with DNase I (Boehringer, Ingelheim, Germany) and reverse transcription was performed with Oligo(dT)18, Superscript™ II RNase H- reverse transcriptase (Invitrogen, Carlsbad, CA), DTT and dNTP mixture.

Modification:

Article Title: Soluble galectin-3 is a strong, colonic epithelial-cell-derived, lamina propria fibroblast-stimulating factor
Article Snippet: The remaining tissue was rinsed and digested for 30 min at 37°C with 1 mg/ml collagenase 1 (Sigma), 0.3 mg/ml DNase I (Boehringer Ingelheim, Ingelheim, Germany) and 2 mg/ml hyaluronidase (Sigma) in phosphate‐buffered saline (PBS; Gibco, Karlsruhe, Germany). .. The isolated cells were cultured in 25 cm2 cell culture flasks (Costar, Bodenheim, Germany) with Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal calf serum (FCS; Pan Biotech, Aidenbach, Germany), penicillin 100 IE/ml (PAA Laboratories), streptomycin 100 µ g/ml (PAA Laboratories), ciprofloxacin 8 µg/ml (Bayer, Leverkusen, Germany), gentamycin 50 µg/ml (PAA laboratories) and amphotericin B 1 µg/ml (Biochrom, Berlin, Germany).

Cell Counting:

Article Title: Cigarette smoke enhances Th-2 driven airway inflammation and delays inhalational tolerance
Article Snippet: The right lung was thoroughly minced and incubated in digestion medium at 37°C containing DNase I (grade II from bovine pancreas; Boehringer Ingelheim, Germany) and collagenase type II (Worthington Biochemical Corp., Lakewood, NJ). .. Cell counting was performed with a Z2 Beckman-Coulter particle counter (Beckman-Coulter, Ghent, Belgium).

Microscopy:

Article Title: Selective Over-Expression of Endothelin-1 in Endothelial Cells Exacerbates Inner Retinal Edema and Neuronal Death in Ischemic Retina
Article Snippet: Semi-quantitative reverse transcriptase-PCR (rt-PCR) of ET-1 gene Mice at the age of 6 to 8 weeks were sacrificed, their eyes were cut underneath the optic nerve and the retinae were dissected under dissecting microscope (Stemi SV6; Carl Zeiss, Thornwood, NY), frozen in liquid nitrogen and stored in a −80°C. .. 1.5 µg of total RNA was treated with DNase I (Boehringer, Ingelheim, Germany) and reverse transcription was performed with Oligo(dT)18, Superscript™ II RNase H- reverse transcriptase (Invitrogen, Carlsbad, CA), DTT and dNTP mixture.

Expressing:

Article Title: Selective Over-Expression of Endothelin-1 in Endothelial Cells Exacerbates Inner Retinal Edema and Neuronal Death in Ischemic Retina
Article Snippet: 1.5 µg of total RNA was treated with DNase I (Boehringer, Ingelheim, Germany) and reverse transcription was performed with Oligo(dT)18, Superscript™ II RNase H- reverse transcriptase (Invitrogen, Carlsbad, CA), DTT and dNTP mixture. .. The ratio of intensities of ET-1 to GAPDH genes expression of each sample was determined by densitometer.

Protein Concentration:

Article Title: Loss of Aip1 reveals a role in maintaining the actin monomer pool and an in vivo oligomer assembly pathway
Article Snippet: Protein concentration was quantified in triplicate using protein assay (Bio-Rad Laboratories), and extracts were normalized to 10 mg/ml using FSB + 30 µM phalloidin. .. Extracts were used immediately and were not allowed to remain on ice for > 1 h. 200 µl of 100 nM DNase I (Boehringer Ingelheim) was added to 800 µl of 25 µg/ml herring sperm DNA.

Reverse Transcription Polymerase Chain Reaction:

Article Title: Selective Over-Expression of Endothelin-1 in Endothelial Cells Exacerbates Inner Retinal Edema and Neuronal Death in Ischemic Retina
Article Snippet: Paragraph title: Semi-quantitative reverse transcriptase-PCR (rt-PCR) of ET-1 gene ... 1.5 µg of total RNA was treated with DNase I (Boehringer, Ingelheim, Germany) and reverse transcription was performed with Oligo(dT)18, Superscript™ II RNase H- reverse transcriptase (Invitrogen, Carlsbad, CA), DTT and dNTP mixture.

FACS:

Article Title: Spatiotemporally separated antigen uptake by alveolar dendritic cells and airway presentation to T cells in the lung
Article Snippet: Lung lobes and trachea were separated and placed into dissociation medium composed of RPMI with 10% FCS, 400 U/ml collagenase D (Roche), and 0.25 mg/ml DNase I (Boehringer Ingelheim). .. Resulting cells were filtered through a 100-µm filter, separated from dead cells and debris with a Histopaque (Sigma-Aldrich) gradient, and stained for FACS or sorting.

Lysis:

Article Title: Characterization and Quantification of Innate Lymphoid Cell Subsets in Human Lung
Article Snippet: The lung tissue was cut into fine pieces and digested for 45 minutes at 37°C in digestion medium (Roswell Park Memorial Institute medium (RPMI) 1640 supplemented with 5% fetal calf serum (FCS), 2 mM L-glutamine, 0.05 mM 2-mercaptomethanol (all Invitrogen), 100 U/ml penicillin, 100 mg/ml streptomycin (Sigma-Aldrich), 1 mg/ml collagenase type 2 (Worthington Biochemical), and 0.02 mg/ml DNase I (grade II from bovine pancreas; Boehringer Ingelheim)). .. Finally, cells were subjected to red blood cell lysis ( ).

Article Title: Cigarette smoke enhances Th-2 driven airway inflammation and delays inhalational tolerance
Article Snippet: The right lung was thoroughly minced and incubated in digestion medium at 37°C containing DNase I (grade II from bovine pancreas; Boehringer Ingelheim, Germany) and collagenase type II (Worthington Biochemical Corp., Lakewood, NJ). .. Finally, cells were subjected to red blood cell lysis, washed and kept on ice until labelling.

Gradient Centrifugation:

Article Title: Human lymphocyte stimulation with pouchitis flora is greater than with flora from a healthy pouch but is suppressed by metronidazole
Article Snippet: LPMC: biopsies were collected into HBSS-CMF (Gibco) and immediately treated with DTT and EDTA followed by a 14 hour collagenase digestion with DNAase I (Boehringer Ingelheim, Bracknell, UK) in AIM V lymphocyte medium (Gibco). .. Medium and cells were collected through a cell strainer (Falcon, via Marathon LS, London, UK) and washed before density gradient centrifugation over Ficoll-paque plus (Amersham Pharmacia Biotech, St Alban’s, UK).

Inhibition:

Article Title: Loss of Aip1 reveals a role in maintaining the actin monomer pool and an in vivo oligomer assembly pathway
Article Snippet: Paragraph title: DNase I inhibition assays ... Extracts were used immediately and were not allowed to remain on ice for > 1 h. 200 µl of 100 nM DNase I (Boehringer Ingelheim) was added to 800 µl of 25 µg/ml herring sperm DNA.

Staining:

Article Title: Spatiotemporally separated antigen uptake by alveolar dendritic cells and airway presentation to T cells in the lung
Article Snippet: Lung lobes and trachea were separated and placed into dissociation medium composed of RPMI with 10% FCS, 400 U/ml collagenase D (Roche), and 0.25 mg/ml DNase I (Boehringer Ingelheim). .. Resulting cells were filtered through a 100-µm filter, separated from dead cells and debris with a Histopaque (Sigma-Aldrich) gradient, and stained for FACS or sorting.

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    Boehringer Ingelheim dnase i
    Dnase I, supplied by Boehringer Ingelheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase i/product/Boehringer Ingelheim
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dnase i - by Bioz Stars, 2020-04
    86/100 stars
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