dna spectrometer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher dna spectrometer
    Dna Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Related Articles

    Amplification:

    Article Title: Identification of Single Nucleotide Polymorphism Markers in the Laccase Gene of Shiitake Mushrooms (Lentinula edodes)
    Article Snippet: Total DNA was quantified using a DNA spectrometer (Nano-Drop ND-1000; NanoDrop Technologies, Wilmington, DE, USA). .. The laccase gene was then amplified with our newly developed primers using PCR conditions as follows: 3min denaturation at 95℃, 30 cycles of 1min denaturation at 94℃, 1 min annealing at 52℃ (T a ), and 1 min extension at 72℃, and finally, 5 min extension at 72℃.

    Isolation:

    Article Title: Identification of Single Nucleotide Polymorphism Markers in the Laccase Gene of Shiitake Mushrooms (Lentinula edodes)
    Article Snippet: Genomic DNA was isolated from a small piece of shiitake mycelia using the phenol-chloroform method or a plant genomic DNA kit (Bionics, Seoul, Korea). .. Total DNA was quantified using a DNA spectrometer (Nano-Drop ND-1000; NanoDrop Technologies, Wilmington, DE, USA).

    Sequencing:

    Article Title: Identification of Single Nucleotide Polymorphism Markers in the Laccase Gene of Shiitake Mushrooms (Lentinula edodes)
    Article Snippet: Total DNA was quantified using a DNA spectrometer (Nano-Drop ND-1000; NanoDrop Technologies, Wilmington, DE, USA). .. 0.4.0, based on the sequence data deposited in GenBank (accession No. FJ473386) ( ).

    Incubation:

    Article Title: Identification of Single Nucleotide Polymorphism Markers in the Laccase Gene of Shiitake Mushrooms (Lentinula edodes)
    Article Snippet: Liquid cultures of the fungi using potato dextrose broth were incubated at 25℃ for about 30 days, after which the mycelia were harvested from the medium. .. Total DNA was quantified using a DNA spectrometer (Nano-Drop ND-1000; NanoDrop Technologies, Wilmington, DE, USA).

    Polymerase Chain Reaction:

    Article Title: Identification of Single Nucleotide Polymorphism Markers in the Laccase Gene of Shiitake Mushrooms (Lentinula edodes)
    Article Snippet: Total DNA was quantified using a DNA spectrometer (Nano-Drop ND-1000; NanoDrop Technologies, Wilmington, DE, USA). .. The laccase gene was then amplified with our newly developed primers using PCR conditions as follows: 3min denaturation at 95℃, 30 cycles of 1min denaturation at 94℃, 1 min annealing at 52℃ (T a ), and 1 min extension at 72℃, and finally, 5 min extension at 72℃.

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    Thermo Fisher isolate ys3
    The least energy structures of the sophorolipid (SL) homologues detected during LC–MS analyses of the SL produced by Rhodotorula babjevae <t>YS3</t> and SL standard, 1,4′′-sophorolactone 6′,6′′-diacetate. Structures were drawn in ChemDraw Ultra 12.0 ( LS lactonic SL, AS acidic SL, Ac acetyl group)
    Isolate Ys3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dna protein complexes
    Effects of deletions within the ENII/BCP sequence on down-regulation of the ENII/BCP activity mediated by LUC7L3. ( A ) Deletion constructs of the ENII/BCP region of the HBV genome linked to the firefly luciferase gene are shown. Cells were co-transfected with one of these reporter constructs and the LUC7L3-expressing plasmid or an empty vector. At 48 h post-transfection, cells were harvested and subjected to the luciferase assay. Values obtained without LUC7L3 expression were set as 1 for expression of each reporter. Results represent the means with SEM from three independent transfectants. ( B ) Interaction of LUC7L3 with the ENII/BCP sequence but not with its deletion, del4 as indicated in ( A ), was shown by <t>DNA</t> pull-down assay. In vitro synthesized biotinylated DNA derived from entire ENII/BCP or its deletion of nt 1666–1700 region was mixed with the nuclear extract prepared from cells transfected with LUC7L3 construct. Biotinylated DNA-protein complexes were captured with <t>streptavidin-conjugated</t> beads, followed by immunoblotting with anti-FLAG antibody. ( C ) Effects of knockdown of HNF4α or C/EBPα on down-regulation of the ENII/BCP activity and the pgRNA expression were evaluated. At 24 h after introducing siRNA against HNF4α (siHNF4a) or C/EBPα (siC/EBPa) or negative control (siCont), cells were transfected with pUC-HB-Ce or pGLHBp1627/1817 together with the LUC7L3-expressing plasmid (LUC7) or an empty vector (EV) and cultured for further 48 h. The reporter activities (upper left) and the pgRNA levels (upper right) were determined by the luciferase assay and RT-qPCR, respectively. Knockdown efficiency of HNF4α (lower left) and C/EBPα (lower right) was assessed by RT-qPCR. Results represent the means with SEM from three independent transfectants.
    Dna Protein Complexes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher total dna pkcs
    <t>DDR/DNA</t> repair proteins show suboptimal activation in TICS compared with bulk cells. ( a ) Western blot analysis of H125 bulk cells and TICs, 1, 4 and 24 h after IR, and A549, H1299 and H23 2 h after IR with 8 Gy. Phosphorylated and total forms of DNA-PK, ATM and phosphorylated forms of the ATM substrates H2AX, KAP1 and Chk2 were analyzed. α -Tubulin or glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as loading controls. ( b ) Western blot analysis of H125 bulk cells and TICs, 4 h after bleomycin (2.5 μg/ml) or 24 h after cisplatin (1 or 10 μ M) exposure. Phosphorylated and total forms of DNA-PK, ATM, phosphorylated forms of the ATM substrates H2AX, KAP1, SMC1, Chk2 as well as the levels of FANCD2 and Rad51 were analyzed. A representative blot of at least two independent experiments is shown. β -Tubulin or GAPDH was used as loading controls. ( c ) Western blot analysis of H125 and A549 bulk cells and TICs, 24 h after 8 Gy IR, or 1 or 10 μ M continuous cisplatin exposure. FANCD2 and cdc25A was assayed. A representative blot of two independent experiments is shown. GAPDH was used as loading control. ( d ) Western blot analysis of PARP full-length and cleavage fragment 24 h after 10 μ M of continuous cisplatin treatment, with or without 1 h pretreatment with <t>DNA-PKcs</t> (NU7026) or ATM (KU55933) inhibitors (both 10 μ M). A representative blot of two independent experiments is shown. GAPDH was used as a loading control
    Total Dna Pkcs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 82/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher putative transgenic cotton plants
    a , b , c, and d Fluorescence in situ hybridization (FISH) of RNAi of two different plants Construct 2 i.e., MC2-8B ( a and b ) and VC2-11 ( c and d ). a Metastatic data of MC2-8B <t>transgenic</t> plant, b Karyotping of RNAi transgene of transgenic plant MC2-8B transgenic plant, c Metastatic data of VC2-11 transgenic plant, d Karyotping of RNAi transgene of transgenic plant VC2-11 transgenic plant
    Putative Transgenic Cotton Plants, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    The least energy structures of the sophorolipid (SL) homologues detected during LC–MS analyses of the SL produced by Rhodotorula babjevae YS3 and SL standard, 1,4′′-sophorolactone 6′,6′′-diacetate. Structures were drawn in ChemDraw Ultra 12.0 ( LS lactonic SL, AS acidic SL, Ac acetyl group)

    Journal: Microbial Cell Factories

    Article Title: Production, characterization, and antifungal activity of a biosurfactant produced by Rhodotorula babjevae YS3

    doi: 10.1186/s12934-017-0711-z

    Figure Lengend Snippet: The least energy structures of the sophorolipid (SL) homologues detected during LC–MS analyses of the SL produced by Rhodotorula babjevae YS3 and SL standard, 1,4′′-sophorolactone 6′,6′′-diacetate. Structures were drawn in ChemDraw Ultra 12.0 ( LS lactonic SL, AS acidic SL, Ac acetyl group)

    Article Snippet: Gene sequencing and phylogenetic analysis of the yeast The genomic DNA of the selected isolate YS3 was extracted using a kit purchased from Thermo Fisher Scientific (Cat No: 7870) according to manufacturer’s protocol.

    Techniques: Liquid Chromatography with Mass Spectroscopy, Produced

    TLC chromatogram showing the separation of components of sophorolipid biosurfactant produced by Rhodotorula babjevae YS3 (SL-YS3) in comparison to the sophorolipid standard, 1,4′′-sophorolactone 6′,6′′-diacetate (SL-S)

    Journal: Microbial Cell Factories

    Article Title: Production, characterization, and antifungal activity of a biosurfactant produced by Rhodotorula babjevae YS3

    doi: 10.1186/s12934-017-0711-z

    Figure Lengend Snippet: TLC chromatogram showing the separation of components of sophorolipid biosurfactant produced by Rhodotorula babjevae YS3 (SL-YS3) in comparison to the sophorolipid standard, 1,4′′-sophorolactone 6′,6′′-diacetate (SL-S)

    Article Snippet: Gene sequencing and phylogenetic analysis of the yeast The genomic DNA of the selected isolate YS3 was extracted using a kit purchased from Thermo Fisher Scientific (Cat No: 7870) according to manufacturer’s protocol.

    Techniques: Thin Layer Chromatography, Produced

    FTIR spectra of the biosurfactant produced by Rhodotorula babjevae YS3 (SL-YS3) and sophorolipid standard, 1,4′′-sophorolactone 6′,6′′-diacetate (SL-S)

    Journal: Microbial Cell Factories

    Article Title: Production, characterization, and antifungal activity of a biosurfactant produced by Rhodotorula babjevae YS3

    doi: 10.1186/s12934-017-0711-z

    Figure Lengend Snippet: FTIR spectra of the biosurfactant produced by Rhodotorula babjevae YS3 (SL-YS3) and sophorolipid standard, 1,4′′-sophorolactone 6′,6′′-diacetate (SL-S)

    Article Snippet: Gene sequencing and phylogenetic analysis of the yeast The genomic DNA of the selected isolate YS3 was extracted using a kit purchased from Thermo Fisher Scientific (Cat No: 7870) according to manufacturer’s protocol.

    Techniques: Produced

    Characterization of the biosurfactant (YG) produced by Rhodotorula babjevae YS3 with glucose as the sole carbon source using LC–MS in positive electrospray ionization mode (+ESI). a MS showing the sodiated adducts of lactonic sophorolipids (LS) with pentadecatrienoic (C 15:3 ) and octadecadienoic (C 18:2 ) lipid side chains at m/z values 581 and 625 respectively, while the adduct ion at m/z 713 represents di-acetylated lactonic sophorolipid (Ac 2 LS) with octadecanoic (C 18 ) lipid chain. b The ion at m/z 579 corresponds to lactonic sophorolipid with hexadecanoic lipid chain (LS-C 16 ). c Disodiated adduct ion of the fragmented tridecenoic fatty acid side chain was observed at m/z 257, the protonated ion at m/z 374 represents the acidic sophorolipid with tridecenoic acidic chain (AS-C 13:1 ) after loss of the terminal hexose (C 6 H 11 O 6 ) from the sophorose disaccharide, sodiated adduct of fragmented mono-acetylated disaccharide moiety (AcSophorose) was observed at m/z 407. d The protonated ions at m/z 285 and 535 represent fragmented octadecanoic fatty acid side chain and lactonic sophorolipid with tridecenoic lipid chain (LS-C 13:1 ) respectively; the sodiated adduct of fragmented di-acetylated sophorose moiety (Ac 2 Sophorose) was observed at m/z 449. e The sodiated adducts of the fragmented sophorose moiety and the acidic sophorolipid with undecanoic acidic side chain (AS-C 11 ) were observed at m/z 365 and 549 respectively

    Journal: Microbial Cell Factories

    Article Title: Production, characterization, and antifungal activity of a biosurfactant produced by Rhodotorula babjevae YS3

    doi: 10.1186/s12934-017-0711-z

    Figure Lengend Snippet: Characterization of the biosurfactant (YG) produced by Rhodotorula babjevae YS3 with glucose as the sole carbon source using LC–MS in positive electrospray ionization mode (+ESI). a MS showing the sodiated adducts of lactonic sophorolipids (LS) with pentadecatrienoic (C 15:3 ) and octadecadienoic (C 18:2 ) lipid side chains at m/z values 581 and 625 respectively, while the adduct ion at m/z 713 represents di-acetylated lactonic sophorolipid (Ac 2 LS) with octadecanoic (C 18 ) lipid chain. b The ion at m/z 579 corresponds to lactonic sophorolipid with hexadecanoic lipid chain (LS-C 16 ). c Disodiated adduct ion of the fragmented tridecenoic fatty acid side chain was observed at m/z 257, the protonated ion at m/z 374 represents the acidic sophorolipid with tridecenoic acidic chain (AS-C 13:1 ) after loss of the terminal hexose (C 6 H 11 O 6 ) from the sophorose disaccharide, sodiated adduct of fragmented mono-acetylated disaccharide moiety (AcSophorose) was observed at m/z 407. d The protonated ions at m/z 285 and 535 represent fragmented octadecanoic fatty acid side chain and lactonic sophorolipid with tridecenoic lipid chain (LS-C 13:1 ) respectively; the sodiated adduct of fragmented di-acetylated sophorose moiety (Ac 2 Sophorose) was observed at m/z 449. e The sodiated adducts of the fragmented sophorose moiety and the acidic sophorolipid with undecanoic acidic side chain (AS-C 11 ) were observed at m/z 365 and 549 respectively

    Article Snippet: Gene sequencing and phylogenetic analysis of the yeast The genomic DNA of the selected isolate YS3 was extracted using a kit purchased from Thermo Fisher Scientific (Cat No: 7870) according to manufacturer’s protocol.

    Techniques: Produced, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry

    Phylogenetic tree of Rhodotorula babjevae YS3 and its related sequences retrieved from NCBI database

    Journal: Microbial Cell Factories

    Article Title: Production, characterization, and antifungal activity of a biosurfactant produced by Rhodotorula babjevae YS3

    doi: 10.1186/s12934-017-0711-z

    Figure Lengend Snippet: Phylogenetic tree of Rhodotorula babjevae YS3 and its related sequences retrieved from NCBI database

    Article Snippet: Gene sequencing and phylogenetic analysis of the yeast The genomic DNA of the selected isolate YS3 was extracted using a kit purchased from Thermo Fisher Scientific (Cat No: 7870) according to manufacturer’s protocol.

    Techniques:

    Growth kinetics, pH, surface tension, and biosurfactant yield of Rhodotorula babjevae YS3 grown at 19 °C, 200 rpm, 5% inoculum (v/v), 10% glucose (w/v) plotted as a function of time. Error bars illustrate standard error of mean (SEM), calculated from two independent experiments in triplicates

    Journal: Microbial Cell Factories

    Article Title: Production, characterization, and antifungal activity of a biosurfactant produced by Rhodotorula babjevae YS3

    doi: 10.1186/s12934-017-0711-z

    Figure Lengend Snippet: Growth kinetics, pH, surface tension, and biosurfactant yield of Rhodotorula babjevae YS3 grown at 19 °C, 200 rpm, 5% inoculum (v/v), 10% glucose (w/v) plotted as a function of time. Error bars illustrate standard error of mean (SEM), calculated from two independent experiments in triplicates

    Article Snippet: Gene sequencing and phylogenetic analysis of the yeast The genomic DNA of the selected isolate YS3 was extracted using a kit purchased from Thermo Fisher Scientific (Cat No: 7870) according to manufacturer’s protocol.

    Techniques:

    Effect of NaCl concentration (%) ( a ), pH ( b ), and heating time (min) ( c ) at 120 °C on ST of the culture supernatants of Rhodotorula babjevae YS3 grown at 19 °C, 200 rpm, 5% inoculum (v/v), 10% glucose (w/v). Error bars illustrate standard error of mean (SEM), calculated from two independent experiments in triplicates

    Journal: Microbial Cell Factories

    Article Title: Production, characterization, and antifungal activity of a biosurfactant produced by Rhodotorula babjevae YS3

    doi: 10.1186/s12934-017-0711-z

    Figure Lengend Snippet: Effect of NaCl concentration (%) ( a ), pH ( b ), and heating time (min) ( c ) at 120 °C on ST of the culture supernatants of Rhodotorula babjevae YS3 grown at 19 °C, 200 rpm, 5% inoculum (v/v), 10% glucose (w/v). Error bars illustrate standard error of mean (SEM), calculated from two independent experiments in triplicates

    Article Snippet: Gene sequencing and phylogenetic analysis of the yeast The genomic DNA of the selected isolate YS3 was extracted using a kit purchased from Thermo Fisher Scientific (Cat No: 7870) according to manufacturer’s protocol.

    Techniques: Concentration Assay

    CMC and minimum surface tension of the biosurfactant produced by Rhodotorula babjevae YS3. Arrow depicts the CMC value

    Journal: Microbial Cell Factories

    Article Title: Production, characterization, and antifungal activity of a biosurfactant produced by Rhodotorula babjevae YS3

    doi: 10.1186/s12934-017-0711-z

    Figure Lengend Snippet: CMC and minimum surface tension of the biosurfactant produced by Rhodotorula babjevae YS3. Arrow depicts the CMC value

    Article Snippet: Gene sequencing and phylogenetic analysis of the yeast The genomic DNA of the selected isolate YS3 was extracted using a kit purchased from Thermo Fisher Scientific (Cat No: 7870) according to manufacturer’s protocol.

    Techniques: Produced

    Effects of deletions within the ENII/BCP sequence on down-regulation of the ENII/BCP activity mediated by LUC7L3. ( A ) Deletion constructs of the ENII/BCP region of the HBV genome linked to the firefly luciferase gene are shown. Cells were co-transfected with one of these reporter constructs and the LUC7L3-expressing plasmid or an empty vector. At 48 h post-transfection, cells were harvested and subjected to the luciferase assay. Values obtained without LUC7L3 expression were set as 1 for expression of each reporter. Results represent the means with SEM from three independent transfectants. ( B ) Interaction of LUC7L3 with the ENII/BCP sequence but not with its deletion, del4 as indicated in ( A ), was shown by DNA pull-down assay. In vitro synthesized biotinylated DNA derived from entire ENII/BCP or its deletion of nt 1666–1700 region was mixed with the nuclear extract prepared from cells transfected with LUC7L3 construct. Biotinylated DNA-protein complexes were captured with streptavidin-conjugated beads, followed by immunoblotting with anti-FLAG antibody. ( C ) Effects of knockdown of HNF4α or C/EBPα on down-regulation of the ENII/BCP activity and the pgRNA expression were evaluated. At 24 h after introducing siRNA against HNF4α (siHNF4a) or C/EBPα (siC/EBPa) or negative control (siCont), cells were transfected with pUC-HB-Ce or pGLHBp1627/1817 together with the LUC7L3-expressing plasmid (LUC7) or an empty vector (EV) and cultured for further 48 h. The reporter activities (upper left) and the pgRNA levels (upper right) were determined by the luciferase assay and RT-qPCR, respectively. Knockdown efficiency of HNF4α (lower left) and C/EBPα (lower right) was assessed by RT-qPCR. Results represent the means with SEM from three independent transfectants.

    Journal: Scientific Reports

    Article Title: LUC7L3/CROP inhibits replication of hepatitis B virus via suppressing enhancer II/basal core promoter activity

    doi: 10.1038/srep36741

    Figure Lengend Snippet: Effects of deletions within the ENII/BCP sequence on down-regulation of the ENII/BCP activity mediated by LUC7L3. ( A ) Deletion constructs of the ENII/BCP region of the HBV genome linked to the firefly luciferase gene are shown. Cells were co-transfected with one of these reporter constructs and the LUC7L3-expressing plasmid or an empty vector. At 48 h post-transfection, cells were harvested and subjected to the luciferase assay. Values obtained without LUC7L3 expression were set as 1 for expression of each reporter. Results represent the means with SEM from three independent transfectants. ( B ) Interaction of LUC7L3 with the ENII/BCP sequence but not with its deletion, del4 as indicated in ( A ), was shown by DNA pull-down assay. In vitro synthesized biotinylated DNA derived from entire ENII/BCP or its deletion of nt 1666–1700 region was mixed with the nuclear extract prepared from cells transfected with LUC7L3 construct. Biotinylated DNA-protein complexes were captured with streptavidin-conjugated beads, followed by immunoblotting with anti-FLAG antibody. ( C ) Effects of knockdown of HNF4α or C/EBPα on down-regulation of the ENII/BCP activity and the pgRNA expression were evaluated. At 24 h after introducing siRNA against HNF4α (siHNF4a) or C/EBPα (siC/EBPa) or negative control (siCont), cells were transfected with pUC-HB-Ce or pGLHBp1627/1817 together with the LUC7L3-expressing plasmid (LUC7) or an empty vector (EV) and cultured for further 48 h. The reporter activities (upper left) and the pgRNA levels (upper right) were determined by the luciferase assay and RT-qPCR, respectively. Knockdown efficiency of HNF4α (lower left) and C/EBPα (lower right) was assessed by RT-qPCR. Results represent the means with SEM from three independent transfectants.

    Article Snippet: The streptavidin-conjugated microbeads were used to isolate DNA-protein complexes, followed by mass-spectrometry (MS) analysis to identify proteins using mass spectrometry Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo fisher, CA, USA) with Xcalibur (version 2.2).

    Techniques: Sequencing, Activity Assay, Construct, Luciferase, Transfection, Expressing, Plasmid Preparation, Pull Down Assay, In Vitro, Synthesized, Derivative Assay, Negative Control, Cell Culture, Quantitative RT-PCR

    DDR/DNA repair proteins show suboptimal activation in TICS compared with bulk cells. ( a ) Western blot analysis of H125 bulk cells and TICs, 1, 4 and 24 h after IR, and A549, H1299 and H23 2 h after IR with 8 Gy. Phosphorylated and total forms of DNA-PK, ATM and phosphorylated forms of the ATM substrates H2AX, KAP1 and Chk2 were analyzed. α -Tubulin or glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as loading controls. ( b ) Western blot analysis of H125 bulk cells and TICs, 4 h after bleomycin (2.5 μg/ml) or 24 h after cisplatin (1 or 10 μ M) exposure. Phosphorylated and total forms of DNA-PK, ATM, phosphorylated forms of the ATM substrates H2AX, KAP1, SMC1, Chk2 as well as the levels of FANCD2 and Rad51 were analyzed. A representative blot of at least two independent experiments is shown. β -Tubulin or GAPDH was used as loading controls. ( c ) Western blot analysis of H125 and A549 bulk cells and TICs, 24 h after 8 Gy IR, or 1 or 10 μ M continuous cisplatin exposure. FANCD2 and cdc25A was assayed. A representative blot of two independent experiments is shown. GAPDH was used as loading control. ( d ) Western blot analysis of PARP full-length and cleavage fragment 24 h after 10 μ M of continuous cisplatin treatment, with or without 1 h pretreatment with DNA-PKcs (NU7026) or ATM (KU55933) inhibitors (both 10 μ M). A representative blot of two independent experiments is shown. GAPDH was used as a loading control

    Journal: Cell Death & Disease

    Article Title: Resistance to DNA-damaging treatment in non-small cell lung cancer tumor-initiating cells involves reduced DNA-PK/ATM activation and diminished cell cycle arrest

    doi: 10.1038/cddis.2012.211

    Figure Lengend Snippet: DDR/DNA repair proteins show suboptimal activation in TICS compared with bulk cells. ( a ) Western blot analysis of H125 bulk cells and TICs, 1, 4 and 24 h after IR, and A549, H1299 and H23 2 h after IR with 8 Gy. Phosphorylated and total forms of DNA-PK, ATM and phosphorylated forms of the ATM substrates H2AX, KAP1 and Chk2 were analyzed. α -Tubulin or glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as loading controls. ( b ) Western blot analysis of H125 bulk cells and TICs, 4 h after bleomycin (2.5 μg/ml) or 24 h after cisplatin (1 or 10 μ M) exposure. Phosphorylated and total forms of DNA-PK, ATM, phosphorylated forms of the ATM substrates H2AX, KAP1, SMC1, Chk2 as well as the levels of FANCD2 and Rad51 were analyzed. A representative blot of at least two independent experiments is shown. β -Tubulin or GAPDH was used as loading controls. ( c ) Western blot analysis of H125 and A549 bulk cells and TICs, 24 h after 8 Gy IR, or 1 or 10 μ M continuous cisplatin exposure. FANCD2 and cdc25A was assayed. A representative blot of two independent experiments is shown. GAPDH was used as loading control. ( d ) Western blot analysis of PARP full-length and cleavage fragment 24 h after 10 μ M of continuous cisplatin treatment, with or without 1 h pretreatment with DNA-PKcs (NU7026) or ATM (KU55933) inhibitors (both 10 μ M). A representative blot of two independent experiments is shown. GAPDH was used as a loading control

    Article Snippet: The following primary antibodies were used: γ H2AX and pDNA-PKcs (pSer139; ab2893 and pSer2056; ab18192, respectively; Abcam), total DNA-PKcs (MS-423P0; Thermo Fisher Scientific, Fremont, CA, USA), pATM (pSer1981; 05-740; Upstate/Millipore, Solna, Sweden), total ATM and FANCD2 (1549-1 and 2986-1, respectively; Epitomics, Burlingame, CA, USA), pKAP1 and pSMC1 (pSer824; A300-767A and pSer966; A300-050A, respectively; Bethyl Laboratories, Montgomery, TX, USA).

    Techniques: Activation Assay, Western Blot

    a , b , c, and d Fluorescence in situ hybridization (FISH) of RNAi of two different plants Construct 2 i.e., MC2-8B ( a and b ) and VC2-11 ( c and d ). a Metastatic data of MC2-8B transgenic plant, b Karyotping of RNAi transgene of transgenic plant MC2-8B transgenic plant, c Metastatic data of VC2-11 transgenic plant, d Karyotping of RNAi transgene of transgenic plant VC2-11 transgenic plant

    Journal: Molecular Biotechnology

    Article Title: Amplicon-Based RNA Interference Targeting V2 Gene of Cotton Leaf Curl Kokhran Virus-Burewala Strain Can Provide Resistance in Transgenic Cotton Plants

    doi: 10.1007/s12033-016-9980-8

    Figure Lengend Snippet: a , b , c, and d Fluorescence in situ hybridization (FISH) of RNAi of two different plants Construct 2 i.e., MC2-8B ( a and b ) and VC2-11 ( c and d ). a Metastatic data of MC2-8B transgenic plant, b Karyotping of RNAi transgene of transgenic plant MC2-8B transgenic plant, c Metastatic data of VC2-11 transgenic plant, d Karyotping of RNAi transgene of transgenic plant VC2-11 transgenic plant

    Article Snippet: Genomic DNA from apical leaves of putative transgenic cotton plants and untransformed control plants was isolated using Thermo scientific Genomic DNA purification kit (cat # K0512) by following the manufacturer’s guidelines.

    Techniques: Fluorescence, In Situ Hybridization, Fluorescence In Situ Hybridization, Construct, Transgenic Assay

    a and b Southern Blot Analysis and Restriction digestion gel of Southern for RNAi in transgenic Cotton plant Lane 1 1 Kb DNA Ladder, Lane 2 MC2-8B transgenic cotton plant of MNH 786 variety, Lane 3 VC2-11 transgenic cotton plant of variety VH-289, Lane 4 Non-transgenic cotton control plant

    Journal: Molecular Biotechnology

    Article Title: Amplicon-Based RNA Interference Targeting V2 Gene of Cotton Leaf Curl Kokhran Virus-Burewala Strain Can Provide Resistance in Transgenic Cotton Plants

    doi: 10.1007/s12033-016-9980-8

    Figure Lengend Snippet: a and b Southern Blot Analysis and Restriction digestion gel of Southern for RNAi in transgenic Cotton plant Lane 1 1 Kb DNA Ladder, Lane 2 MC2-8B transgenic cotton plant of MNH 786 variety, Lane 3 VC2-11 transgenic cotton plant of variety VH-289, Lane 4 Non-transgenic cotton control plant

    Article Snippet: Genomic DNA from apical leaves of putative transgenic cotton plants and untransformed control plants was isolated using Thermo scientific Genomic DNA purification kit (cat # K0512) by following the manufacturer’s guidelines.

    Techniques: Southern Blot, Transgenic Assay

    Pictorial View of Onset of CLCuV on T1 generation observed in different months. a Mild Symptoms of CLCuV on transgenic Cotton Plants. b Persistence of Mild Symptoms (CLCuV Tolerance) by Transgenic Cotton Plants. c Recovery of Plants vegetatively and reproductively while tolerating and minimizing viral titer. d Cotton Plants at Maturity showing tolerance of CLCuV with mild symptoms

    Journal: Molecular Biotechnology

    Article Title: Amplicon-Based RNA Interference Targeting V2 Gene of Cotton Leaf Curl Kokhran Virus-Burewala Strain Can Provide Resistance in Transgenic Cotton Plants

    doi: 10.1007/s12033-016-9980-8

    Figure Lengend Snippet: Pictorial View of Onset of CLCuV on T1 generation observed in different months. a Mild Symptoms of CLCuV on transgenic Cotton Plants. b Persistence of Mild Symptoms (CLCuV Tolerance) by Transgenic Cotton Plants. c Recovery of Plants vegetatively and reproductively while tolerating and minimizing viral titer. d Cotton Plants at Maturity showing tolerance of CLCuV with mild symptoms

    Article Snippet: Genomic DNA from apical leaves of putative transgenic cotton plants and untransformed control plants was isolated using Thermo scientific Genomic DNA purification kit (cat # K0512) by following the manufacturer’s guidelines.

    Techniques: Transgenic Assay

    a Comparison of disease index of the two varieties of cotton (MNH-786 and VH-289) used for transformation. Y -axis is showing number of plants, while X -axis is showing different disease ratings (0–6) as per Table 4 . b Comparison of virus titer of the two varieties of cotton (MNH-786 and VH-289) used for transformation. Y -axis is showing virus titer in terms of molecules/µl × 10 7 , while X -axis is showing transgenic plants, MC2-2, MC2-8, MC2-7, and MC2-10 of MNH-786 and VC2-9, VC2-11, VC2-2, and VC2-6 of VH-289

    Journal: Molecular Biotechnology

    Article Title: Amplicon-Based RNA Interference Targeting V2 Gene of Cotton Leaf Curl Kokhran Virus-Burewala Strain Can Provide Resistance in Transgenic Cotton Plants

    doi: 10.1007/s12033-016-9980-8

    Figure Lengend Snippet: a Comparison of disease index of the two varieties of cotton (MNH-786 and VH-289) used for transformation. Y -axis is showing number of plants, while X -axis is showing different disease ratings (0–6) as per Table 4 . b Comparison of virus titer of the two varieties of cotton (MNH-786 and VH-289) used for transformation. Y -axis is showing virus titer in terms of molecules/µl × 10 7 , while X -axis is showing transgenic plants, MC2-2, MC2-8, MC2-7, and MC2-10 of MNH-786 and VC2-9, VC2-11, VC2-2, and VC2-6 of VH-289

    Article Snippet: Genomic DNA from apical leaves of putative transgenic cotton plants and untransformed control plants was isolated using Thermo scientific Genomic DNA purification kit (cat # K0512) by following the manufacturer’s guidelines.

    Techniques: Transformation Assay, Transgenic Assay

    Viral Titer calculation via real-Time PCR in T1 transgenic plants GC 2 −ve control, MC 2-2, MC 2-8, MC 2-7, and MC 2-10 Transgenic plants of MNH-786; VC 2-9, VC 2-11, VC 2-2, and VC 2-6 transgenic plants of VH-289 in T1 generation harboring RNAi gene construct

    Journal: Molecular Biotechnology

    Article Title: Amplicon-Based RNA Interference Targeting V2 Gene of Cotton Leaf Curl Kokhran Virus-Burewala Strain Can Provide Resistance in Transgenic Cotton Plants

    doi: 10.1007/s12033-016-9980-8

    Figure Lengend Snippet: Viral Titer calculation via real-Time PCR in T1 transgenic plants GC 2 −ve control, MC 2-2, MC 2-8, MC 2-7, and MC 2-10 Transgenic plants of MNH-786; VC 2-9, VC 2-11, VC 2-2, and VC 2-6 transgenic plants of VH-289 in T1 generation harboring RNAi gene construct

    Article Snippet: Genomic DNA from apical leaves of putative transgenic cotton plants and untransformed control plants was isolated using Thermo scientific Genomic DNA purification kit (cat # K0512) by following the manufacturer’s guidelines.

    Techniques: Real-time Polymerase Chain Reaction, Transgenic Assay, Construct

    Comparison of viral disease index and virus titer of T1 generation transgenic Plants. GC non-transgenic plant, MC 2-2, MC 2-8, MC 2-7, and MC 2-10 Transgenic plants of MNH-786, VC 2-9, VC 2-11, VC 2-2, and VC 2-6 transgenic plants of VH-289

    Journal: Molecular Biotechnology

    Article Title: Amplicon-Based RNA Interference Targeting V2 Gene of Cotton Leaf Curl Kokhran Virus-Burewala Strain Can Provide Resistance in Transgenic Cotton Plants

    doi: 10.1007/s12033-016-9980-8

    Figure Lengend Snippet: Comparison of viral disease index and virus titer of T1 generation transgenic Plants. GC non-transgenic plant, MC 2-2, MC 2-8, MC 2-7, and MC 2-10 Transgenic plants of MNH-786, VC 2-9, VC 2-11, VC 2-2, and VC 2-6 transgenic plants of VH-289

    Article Snippet: Genomic DNA from apical leaves of putative transgenic cotton plants and untransformed control plants was isolated using Thermo scientific Genomic DNA purification kit (cat # K0512) by following the manufacturer’s guidelines.

    Techniques: Transgenic Assay

    Steps from embryo transformation to shifting of plants in soil pots a Germination of Embryos in sterilized flasks, b Embryos isolation from seeds, c Agrobacterium -treated embryos are co-cultivated on MS medium, d Agrobacterium -treated embryos growing on MS medium (4 days), e Inoculation of embryos in test tubes containing MS media with kanamycin selection, f Roots developing in MS rooting medium, g Transgenic plants being shifted in soil pots, h putative transgenic plants in soil pots covered with polythene bags, i Acclimatization of putative transgenic plants

    Journal: Molecular Biotechnology

    Article Title: Amplicon-Based RNA Interference Targeting V2 Gene of Cotton Leaf Curl Kokhran Virus-Burewala Strain Can Provide Resistance in Transgenic Cotton Plants

    doi: 10.1007/s12033-016-9980-8

    Figure Lengend Snippet: Steps from embryo transformation to shifting of plants in soil pots a Germination of Embryos in sterilized flasks, b Embryos isolation from seeds, c Agrobacterium -treated embryos are co-cultivated on MS medium, d Agrobacterium -treated embryos growing on MS medium (4 days), e Inoculation of embryos in test tubes containing MS media with kanamycin selection, f Roots developing in MS rooting medium, g Transgenic plants being shifted in soil pots, h putative transgenic plants in soil pots covered with polythene bags, i Acclimatization of putative transgenic plants

    Article Snippet: Genomic DNA from apical leaves of putative transgenic cotton plants and untransformed control plants was isolated using Thermo scientific Genomic DNA purification kit (cat # K0512) by following the manufacturer’s guidelines.

    Techniques: Transformation Assay, Isolation, Mass Spectrometry, Selection, Transgenic Assay

    PCR analysis of putative transgenic plants (VH-289 MNH-786) L 50 bp DNA ladder; − ve the non-transgenic plant, + ve plasmid construct was used as positive control, V 1– V 6 putative transgenic plants of VH-289, M 1– M 6 putative transgenic plants of MNH-786

    Journal: Molecular Biotechnology

    Article Title: Amplicon-Based RNA Interference Targeting V2 Gene of Cotton Leaf Curl Kokhran Virus-Burewala Strain Can Provide Resistance in Transgenic Cotton Plants

    doi: 10.1007/s12033-016-9980-8

    Figure Lengend Snippet: PCR analysis of putative transgenic plants (VH-289 MNH-786) L 50 bp DNA ladder; − ve the non-transgenic plant, + ve plasmid construct was used as positive control, V 1– V 6 putative transgenic plants of VH-289, M 1– M 6 putative transgenic plants of MNH-786

    Article Snippet: Genomic DNA from apical leaves of putative transgenic cotton plants and untransformed control plants was isolated using Thermo scientific Genomic DNA purification kit (cat # K0512) by following the manufacturer’s guidelines.

    Techniques: Polymerase Chain Reaction, Transgenic Assay, Plasmid Preparation, Construct, Positive Control

    Putative ( T 0 ) plants in field a Newly shifted T 0 transgenic plants, b T 0 plants thriving in the field, c selfing of bolls to self-cross the seeds, d T 0 bolls at maturity, e and f Picking of T 0 bolls

    Journal: Molecular Biotechnology

    Article Title: Amplicon-Based RNA Interference Targeting V2 Gene of Cotton Leaf Curl Kokhran Virus-Burewala Strain Can Provide Resistance in Transgenic Cotton Plants

    doi: 10.1007/s12033-016-9980-8

    Figure Lengend Snippet: Putative ( T 0 ) plants in field a Newly shifted T 0 transgenic plants, b T 0 plants thriving in the field, c selfing of bolls to self-cross the seeds, d T 0 bolls at maturity, e and f Picking of T 0 bolls

    Article Snippet: Genomic DNA from apical leaves of putative transgenic cotton plants and untransformed control plants was isolated using Thermo scientific Genomic DNA purification kit (cat # K0512) by following the manufacturer’s guidelines.

    Techniques: Transgenic Assay