Journal: PLoS ONE

Article Title: Metastable Differentially Methylated Regions within Arabidopsis Inbred Populations Are Associated with Modified Expression of Non-Coding Transcripts

doi: 10.1371/journal.pone.0045242

Figure Lengend Snippet: ( a ) Methylation (estimated by McrBC digestion) of RITA and MRD1 in the AGO5 allelic series. ( b ) McrBC estimation of the methylation levels of RITA and MRD1 in an SDE5 allelic series. ( c ) sRNA northern blot for SDE5 allelic series. miR165 and U6 are loading controls. ( d ) SDE5 allelic series mutant phenotypes. White arrows highlight accelerated juvenile-adult transition phenotype in leaves. ( e ) Methylation of RITA and MRD1 in seedling RNA polymerase mutant alleles. The lines indicated with a * are also known to be mutants in the POLV complex (NRPE). ( f ) DNA methylation of RITA and MRD1 in Col-0 lines obtained from different lab (denoted by the principal investigator’s surname). #’s refer to individual plants; 4 individuals were assayed per line.

Article Snippet: For the McrBC assay, 1000 ng of DNA were diluted in 12.5 µl of DNA Hydration Solution, 1X NEB Buffer 2, 10 µg BSA, 1 mM GTP, and 75.5 µl water.

Techniques: Methylation, Northern Blot, Mutagenesis, DNA Methylation Assay