Structured Review

Volpi AG dna sequences
A range of images displaying characteristics of <t>DNA</t> <t>halo</t> preparations. Nucleoskeleton-associated DNA is anchored to the residual nucleus, while extracted, non-associated DNA forms the faint halo surrounding the structure. DNA has been stained using DAPI and can be seen in blue. Panel a displays a representative DNA Halo preparation that has been subjected to immuno-FISH using a painting probe for chromosome 17 (red) and the proliferation nucleolar marker pKi67 (green). Panel b displays another DNA Halo preparation with again the nucleolar proliferation marker pKi67 in green and CCND1 gene loci in red. Panel c is a DNA Halo preparation displaying a proliferating cell that has incorporated bromo-deoxy-uridine during DNA replication (red), DNA in blue and telomere sequences revealed with a PNA-telo probe in green. Panel d shows a residual nucleus surrounded by a halo of DNA visualised by feSEM. The morphology of the residual nucleus (RN) and surrounding DNA halo (DH) Magnification = 6 k scale bar = 2.5 µm. Panel e displays extracted, supercoiled DNA (white arrowheads) within the DNA halo, scale bar 300 nm. Panels f and g are representative control and HGPS fibroblasts with lamin A revealed by indirect immunofluorescence in unextracted cells, respectively and lamin A distribution present in residual nuclei of DNA halo preparations in extracted cells ( h and i ). Panels j – m display representative images of DNA halo preparations with telomeres in green in proliferating control and HGPS fibroblasts ( j and k , respectively) revealed with anti-Ki67 staining (red) and senescent control and HGPS fibroblasts ( l and m , respectively). Note in the HGPS cells telomere signals are located out with the residual nuclei. Scale bars in panels b and f = 10 μm
Dna Sequences, supplied by Volpi AG, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dna sequences/product/Volpi AG
Average 93 stars, based on 3 article reviews
Price from $9.99 to $1999.99
dna sequences - by Bioz Stars, 2020-09
93/100 stars

Images

1) Product Images from "Farnesyltransferase inhibitor and rapamycin correct aberrant genome organisation and decrease DNA damage respectively, in Hutchinson–Gilford progeria syndrome fibroblasts"

Article Title: Farnesyltransferase inhibitor and rapamycin correct aberrant genome organisation and decrease DNA damage respectively, in Hutchinson–Gilford progeria syndrome fibroblasts

Journal: Biogerontology

doi: 10.1007/s10522-018-9758-4

A range of images displaying characteristics of DNA halo preparations. Nucleoskeleton-associated DNA is anchored to the residual nucleus, while extracted, non-associated DNA forms the faint halo surrounding the structure. DNA has been stained using DAPI and can be seen in blue. Panel a displays a representative DNA Halo preparation that has been subjected to immuno-FISH using a painting probe for chromosome 17 (red) and the proliferation nucleolar marker pKi67 (green). Panel b displays another DNA Halo preparation with again the nucleolar proliferation marker pKi67 in green and CCND1 gene loci in red. Panel c is a DNA Halo preparation displaying a proliferating cell that has incorporated bromo-deoxy-uridine during DNA replication (red), DNA in blue and telomere sequences revealed with a PNA-telo probe in green. Panel d shows a residual nucleus surrounded by a halo of DNA visualised by feSEM. The morphology of the residual nucleus (RN) and surrounding DNA halo (DH) Magnification = 6 k scale bar = 2.5 µm. Panel e displays extracted, supercoiled DNA (white arrowheads) within the DNA halo, scale bar 300 nm. Panels f and g are representative control and HGPS fibroblasts with lamin A revealed by indirect immunofluorescence in unextracted cells, respectively and lamin A distribution present in residual nuclei of DNA halo preparations in extracted cells ( h and i ). Panels j – m display representative images of DNA halo preparations with telomeres in green in proliferating control and HGPS fibroblasts ( j and k , respectively) revealed with anti-Ki67 staining (red) and senescent control and HGPS fibroblasts ( l and m , respectively). Note in the HGPS cells telomere signals are located out with the residual nuclei. Scale bars in panels b and f = 10 μm
Figure Legend Snippet: A range of images displaying characteristics of DNA halo preparations. Nucleoskeleton-associated DNA is anchored to the residual nucleus, while extracted, non-associated DNA forms the faint halo surrounding the structure. DNA has been stained using DAPI and can be seen in blue. Panel a displays a representative DNA Halo preparation that has been subjected to immuno-FISH using a painting probe for chromosome 17 (red) and the proliferation nucleolar marker pKi67 (green). Panel b displays another DNA Halo preparation with again the nucleolar proliferation marker pKi67 in green and CCND1 gene loci in red. Panel c is a DNA Halo preparation displaying a proliferating cell that has incorporated bromo-deoxy-uridine during DNA replication (red), DNA in blue and telomere sequences revealed with a PNA-telo probe in green. Panel d shows a residual nucleus surrounded by a halo of DNA visualised by feSEM. The morphology of the residual nucleus (RN) and surrounding DNA halo (DH) Magnification = 6 k scale bar = 2.5 µm. Panel e displays extracted, supercoiled DNA (white arrowheads) within the DNA halo, scale bar 300 nm. Panels f and g are representative control and HGPS fibroblasts with lamin A revealed by indirect immunofluorescence in unextracted cells, respectively and lamin A distribution present in residual nuclei of DNA halo preparations in extracted cells ( h and i ). Panels j – m display representative images of DNA halo preparations with telomeres in green in proliferating control and HGPS fibroblasts ( j and k , respectively) revealed with anti-Ki67 staining (red) and senescent control and HGPS fibroblasts ( l and m , respectively). Note in the HGPS cells telomere signals are located out with the residual nuclei. Scale bars in panels b and f = 10 μm

Techniques Used: Staining, Fluorescence In Situ Hybridization, Marker, Immunofluorescence

2) Product Images from "Farnesyltransferase inhibitor and rapamycin correct aberrant genome organisation and decrease DNA damage respectively, in Hutchinson–Gilford progeria syndrome fibroblasts"

Article Title: Farnesyltransferase inhibitor and rapamycin correct aberrant genome organisation and decrease DNA damage respectively, in Hutchinson–Gilford progeria syndrome fibroblasts

Journal: Biogerontology

doi: 10.1007/s10522-018-9758-4

Comparisons of the fraction of telomeres found in the residual nuclei of DNA Halo preparations of HGPS before and after drug treatments. Images of fixed control and HGPS DNA halos stained with both DAPI (blue) and Cy3 (red) telomeric PNA probes were captured at ×100 magnification. Residual nuclei were delineated and the fraction of telomeres determined inside and outside of the residual nuclei. Results for the treatments are shown as a 2DD with zoledronic acid, b FTI-277, c pravastatin, d zoledronic acid, e rapamycin, f N -acetyl- l -cysteine, g FTI-277 and GGTI-2133, h pravastatin and zoledronic acid and i FTI-277, pravastatin and zoledronic acid. The proportion of interior telomeres is expressed as a percentage of the total number of telomeres present. Error bars represent ± SEM. Scale bar = 10 µM
Figure Legend Snippet: Comparisons of the fraction of telomeres found in the residual nuclei of DNA Halo preparations of HGPS before and after drug treatments. Images of fixed control and HGPS DNA halos stained with both DAPI (blue) and Cy3 (red) telomeric PNA probes were captured at ×100 magnification. Residual nuclei were delineated and the fraction of telomeres determined inside and outside of the residual nuclei. Results for the treatments are shown as a 2DD with zoledronic acid, b FTI-277, c pravastatin, d zoledronic acid, e rapamycin, f N -acetyl- l -cysteine, g FTI-277 and GGTI-2133, h pravastatin and zoledronic acid and i FTI-277, pravastatin and zoledronic acid. The proportion of interior telomeres is expressed as a percentage of the total number of telomeres present. Error bars represent ± SEM. Scale bar = 10 µM

Techniques Used: Staining

Alterations in size of residual nuclei in DNA Halo preparations of HGPS fibroblasts before and after drug treatments. Images of fixed HGPS DNA halos stained with DAPI taken at ×100 magnification were analysed by ImageJ delineating the residual nuclei and the entire DNA halo. The data displayed in this figure reveals the area measurement of the residual nuclei (x-axis). Results for the treatments are a FTI-277, b pravastatin, c zoledronic acid, d rapamycin, e IGF-1, f N -acetyl- l -cysteine, g FTI-277 and GGTI-2133, h pravastatin and zoledronic acid and i FTI-277, pravastatin and zoledronic acid. Error bars represent ± SEM
Figure Legend Snippet: Alterations in size of residual nuclei in DNA Halo preparations of HGPS fibroblasts before and after drug treatments. Images of fixed HGPS DNA halos stained with DAPI taken at ×100 magnification were analysed by ImageJ delineating the residual nuclei and the entire DNA halo. The data displayed in this figure reveals the area measurement of the residual nuclei (x-axis). Results for the treatments are a FTI-277, b pravastatin, c zoledronic acid, d rapamycin, e IGF-1, f N -acetyl- l -cysteine, g FTI-277 and GGTI-2133, h pravastatin and zoledronic acid and i FTI-277, pravastatin and zoledronic acid. Error bars represent ± SEM

Techniques Used: Staining

A range of images displaying characteristics of DNA halo preparations. Nucleoskeleton-associated DNA is anchored to the residual nucleus, while extracted, non-associated DNA forms the faint halo surrounding the structure. DNA has been stained using DAPI and can be seen in blue. Panel a displays a representative DNA Halo preparation that has been subjected to immuno-FISH using a painting probe for chromosome 17 (red) and the proliferation nucleolar marker pKi67 (green). Panel b displays another DNA Halo preparation with again the nucleolar proliferation marker pKi67 in green and CCND1 gene loci in red. Panel c is a DNA Halo preparation displaying a proliferating cell that has incorporated bromo-deoxy-uridine during DNA replication (red), DNA in blue and telomere sequences revealed with a PNA-telo probe in green. Panel d shows a residual nucleus surrounded by a halo of DNA visualised by feSEM. The morphology of the residual nucleus (RN) and surrounding DNA halo (DH) Magnification = 6 k scale bar = 2.5 µm. Panel e displays extracted, supercoiled DNA (white arrowheads) within the DNA halo, scale bar 300 nm. Panels f and g are representative control and HGPS fibroblasts with lamin A revealed by indirect immunofluorescence in unextracted cells, respectively and lamin A distribution present in residual nuclei of DNA halo preparations in extracted cells ( h and i ). Panels j – m display representative images of DNA halo preparations with telomeres in green in proliferating control and HGPS fibroblasts ( j and k , respectively) revealed with anti-Ki67 staining (red) and senescent control and HGPS fibroblasts ( l and m , respectively). Note in the HGPS cells telomere signals are located out with the residual nuclei. Scale bars in panels b and f = 10 μm
Figure Legend Snippet: A range of images displaying characteristics of DNA halo preparations. Nucleoskeleton-associated DNA is anchored to the residual nucleus, while extracted, non-associated DNA forms the faint halo surrounding the structure. DNA has been stained using DAPI and can be seen in blue. Panel a displays a representative DNA Halo preparation that has been subjected to immuno-FISH using a painting probe for chromosome 17 (red) and the proliferation nucleolar marker pKi67 (green). Panel b displays another DNA Halo preparation with again the nucleolar proliferation marker pKi67 in green and CCND1 gene loci in red. Panel c is a DNA Halo preparation displaying a proliferating cell that has incorporated bromo-deoxy-uridine during DNA replication (red), DNA in blue and telomere sequences revealed with a PNA-telo probe in green. Panel d shows a residual nucleus surrounded by a halo of DNA visualised by feSEM. The morphology of the residual nucleus (RN) and surrounding DNA halo (DH) Magnification = 6 k scale bar = 2.5 µm. Panel e displays extracted, supercoiled DNA (white arrowheads) within the DNA halo, scale bar 300 nm. Panels f and g are representative control and HGPS fibroblasts with lamin A revealed by indirect immunofluorescence in unextracted cells, respectively and lamin A distribution present in residual nuclei of DNA halo preparations in extracted cells ( h and i ). Panels j – m display representative images of DNA halo preparations with telomeres in green in proliferating control and HGPS fibroblasts ( j and k , respectively) revealed with anti-Ki67 staining (red) and senescent control and HGPS fibroblasts ( l and m , respectively). Note in the HGPS cells telomere signals are located out with the residual nuclei. Scale bars in panels b and f = 10 μm

Techniques Used: Staining, Fluorescence In Situ Hybridization, Marker, Immunofluorescence

Related Articles

Sequencing:

Article Title: Transgenic expression of pectin methylesterase inhibitors limits tobamovirus spread in tobacco and Arabidopsis
Article Snippet: .. The DNA sequence containing the coding region of the AcPMEI gene (Di Matteo et al ., ; Volpi et al ., ), fused to the signal peptide of the bean polygalacturonase‐inhibiting protein 1 (Pvpgip1) for apoplastic targeting (Desiderio et al ., ; Volpi et al ), was inserted into the Bam HI and Sac I site of the pBI121(Ω) vector under the control of the CaMV 35S promoter and translation enhancer Ω leader (Hansen et al ., ) (Fig. a). .. The pBI121Ω‐SPPGIP AcPMEI was mobilized into Agrobacterium tumefaciens strain LBA4404 by electroporation.

Article Title: Fluorescence in situ hybridization with high-complexity repeat-free oligonucleotide probes generated by massively parallel synthesis
Article Snippet: .. Introduction Using standard fluorescence in situ hybridization (FISH) probes, specific loci, classes of DNA sequence, genomic regions and even whole chromosomes can be detected (Volpi and Bridger ; Levsky and Singer ). .. FISH probes can be labelled either directly with fluorescent nucleotides or indirectly and then detected with fluorescent antibodies or affinity molecules.

Fluorescence In Situ Hybridization:

Article Title: Farnesyltransferase inhibitor and rapamycin correct aberrant genome organisation and decrease DNA damage respectively, in Hutchinson–Gilford progeria syndrome fibroblasts
Article Snippet: .. Performing FISH on these preparations, named HALO-FISH using telomere specific probes can delineate specific DNA sequences (Volpi and Bridger ; de Lange ). ..

Article Title: Fluorescence in situ hybridization with high-complexity repeat-free oligonucleotide probes generated by massively parallel synthesis
Article Snippet: .. Introduction Using standard fluorescence in situ hybridization (FISH) probes, specific loci, classes of DNA sequence, genomic regions and even whole chromosomes can be detected (Volpi and Bridger ; Levsky and Singer ). .. FISH probes can be labelled either directly with fluorescent nucleotides or indirectly and then detected with fluorescent antibodies or affinity molecules.

Plasmid Preparation:

Article Title: Transgenic expression of pectin methylesterase inhibitors limits tobamovirus spread in tobacco and Arabidopsis
Article Snippet: .. The DNA sequence containing the coding region of the AcPMEI gene (Di Matteo et al ., ; Volpi et al ., ), fused to the signal peptide of the bean polygalacturonase‐inhibiting protein 1 (Pvpgip1) for apoplastic targeting (Desiderio et al ., ; Volpi et al ), was inserted into the Bam HI and Sac I site of the pBI121(Ω) vector under the control of the CaMV 35S promoter and translation enhancer Ω leader (Hansen et al ., ) (Fig. a). .. The pBI121Ω‐SPPGIP AcPMEI was mobilized into Agrobacterium tumefaciens strain LBA4404 by electroporation.

Fluorescence:

Article Title: Fluorescence in situ hybridization with high-complexity repeat-free oligonucleotide probes generated by massively parallel synthesis
Article Snippet: .. Introduction Using standard fluorescence in situ hybridization (FISH) probes, specific loci, classes of DNA sequence, genomic regions and even whole chromosomes can be detected (Volpi and Bridger ; Levsky and Singer ). .. FISH probes can be labelled either directly with fluorescent nucleotides or indirectly and then detected with fluorescent antibodies or affinity molecules.

In Situ Hybridization:

Article Title: Fluorescence in situ hybridization with high-complexity repeat-free oligonucleotide probes generated by massively parallel synthesis
Article Snippet: .. Introduction Using standard fluorescence in situ hybridization (FISH) probes, specific loci, classes of DNA sequence, genomic regions and even whole chromosomes can be detected (Volpi and Bridger ; Levsky and Singer ). .. FISH probes can be labelled either directly with fluorescent nucleotides or indirectly and then detected with fluorescent antibodies or affinity molecules.

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    Volpi AG dna sequences
    A range of images displaying characteristics of <t>DNA</t> <t>halo</t> preparations. Nucleoskeleton-associated DNA is anchored to the residual nucleus, while extracted, non-associated DNA forms the faint halo surrounding the structure. DNA has been stained using DAPI and can be seen in blue. Panel a displays a representative DNA Halo preparation that has been subjected to immuno-FISH using a painting probe for chromosome 17 (red) and the proliferation nucleolar marker pKi67 (green). Panel b displays another DNA Halo preparation with again the nucleolar proliferation marker pKi67 in green and CCND1 gene loci in red. Panel c is a DNA Halo preparation displaying a proliferating cell that has incorporated bromo-deoxy-uridine during DNA replication (red), DNA in blue and telomere sequences revealed with a PNA-telo probe in green. Panel d shows a residual nucleus surrounded by a halo of DNA visualised by feSEM. The morphology of the residual nucleus (RN) and surrounding DNA halo (DH) Magnification = 6 k scale bar = 2.5 µm. Panel e displays extracted, supercoiled DNA (white arrowheads) within the DNA halo, scale bar 300 nm. Panels f and g are representative control and HGPS fibroblasts with lamin A revealed by indirect immunofluorescence in unextracted cells, respectively and lamin A distribution present in residual nuclei of DNA halo preparations in extracted cells ( h and i ). Panels j – m display representative images of DNA halo preparations with telomeres in green in proliferating control and HGPS fibroblasts ( j and k , respectively) revealed with anti-Ki67 staining (red) and senescent control and HGPS fibroblasts ( l and m , respectively). Note in the HGPS cells telomere signals are located out with the residual nuclei. Scale bars in panels b and f = 10 μm
    Dna Sequences, supplied by Volpi AG, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna sequences/product/Volpi AG
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    dna sequences - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

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    A range of images displaying characteristics of DNA halo preparations. Nucleoskeleton-associated DNA is anchored to the residual nucleus, while extracted, non-associated DNA forms the faint halo surrounding the structure. DNA has been stained using DAPI and can be seen in blue. Panel a displays a representative DNA Halo preparation that has been subjected to immuno-FISH using a painting probe for chromosome 17 (red) and the proliferation nucleolar marker pKi67 (green). Panel b displays another DNA Halo preparation with again the nucleolar proliferation marker pKi67 in green and CCND1 gene loci in red. Panel c is a DNA Halo preparation displaying a proliferating cell that has incorporated bromo-deoxy-uridine during DNA replication (red), DNA in blue and telomere sequences revealed with a PNA-telo probe in green. Panel d shows a residual nucleus surrounded by a halo of DNA visualised by feSEM. The morphology of the residual nucleus (RN) and surrounding DNA halo (DH) Magnification = 6 k scale bar = 2.5 µm. Panel e displays extracted, supercoiled DNA (white arrowheads) within the DNA halo, scale bar 300 nm. Panels f and g are representative control and HGPS fibroblasts with lamin A revealed by indirect immunofluorescence in unextracted cells, respectively and lamin A distribution present in residual nuclei of DNA halo preparations in extracted cells ( h and i ). Panels j – m display representative images of DNA halo preparations with telomeres in green in proliferating control and HGPS fibroblasts ( j and k , respectively) revealed with anti-Ki67 staining (red) and senescent control and HGPS fibroblasts ( l and m , respectively). Note in the HGPS cells telomere signals are located out with the residual nuclei. Scale bars in panels b and f = 10 μm

    Journal: Biogerontology

    Article Title: Farnesyltransferase inhibitor and rapamycin correct aberrant genome organisation and decrease DNA damage respectively, in Hutchinson–Gilford progeria syndrome fibroblasts

    doi: 10.1007/s10522-018-9758-4

    Figure Lengend Snippet: A range of images displaying characteristics of DNA halo preparations. Nucleoskeleton-associated DNA is anchored to the residual nucleus, while extracted, non-associated DNA forms the faint halo surrounding the structure. DNA has been stained using DAPI and can be seen in blue. Panel a displays a representative DNA Halo preparation that has been subjected to immuno-FISH using a painting probe for chromosome 17 (red) and the proliferation nucleolar marker pKi67 (green). Panel b displays another DNA Halo preparation with again the nucleolar proliferation marker pKi67 in green and CCND1 gene loci in red. Panel c is a DNA Halo preparation displaying a proliferating cell that has incorporated bromo-deoxy-uridine during DNA replication (red), DNA in blue and telomere sequences revealed with a PNA-telo probe in green. Panel d shows a residual nucleus surrounded by a halo of DNA visualised by feSEM. The morphology of the residual nucleus (RN) and surrounding DNA halo (DH) Magnification = 6 k scale bar = 2.5 µm. Panel e displays extracted, supercoiled DNA (white arrowheads) within the DNA halo, scale bar 300 nm. Panels f and g are representative control and HGPS fibroblasts with lamin A revealed by indirect immunofluorescence in unextracted cells, respectively and lamin A distribution present in residual nuclei of DNA halo preparations in extracted cells ( h and i ). Panels j – m display representative images of DNA halo preparations with telomeres in green in proliferating control and HGPS fibroblasts ( j and k , respectively) revealed with anti-Ki67 staining (red) and senescent control and HGPS fibroblasts ( l and m , respectively). Note in the HGPS cells telomere signals are located out with the residual nuclei. Scale bars in panels b and f = 10 μm

    Article Snippet: Performing FISH on these preparations, named HALO-FISH using telomere specific probes can delineate specific DNA sequences (Volpi and Bridger ; de Lange ).

    Techniques: Staining, Fluorescence In Situ Hybridization, Marker, Immunofluorescence

    Comparisons of the fraction of telomeres found in the residual nuclei of DNA Halo preparations of HGPS before and after drug treatments. Images of fixed control and HGPS DNA halos stained with both DAPI (blue) and Cy3 (red) telomeric PNA probes were captured at ×100 magnification. Residual nuclei were delineated and the fraction of telomeres determined inside and outside of the residual nuclei. Results for the treatments are shown as a 2DD with zoledronic acid, b FTI-277, c pravastatin, d zoledronic acid, e rapamycin, f N -acetyl- l -cysteine, g FTI-277 and GGTI-2133, h pravastatin and zoledronic acid and i FTI-277, pravastatin and zoledronic acid. The proportion of interior telomeres is expressed as a percentage of the total number of telomeres present. Error bars represent ± SEM. Scale bar = 10 µM

    Journal: Biogerontology

    Article Title: Farnesyltransferase inhibitor and rapamycin correct aberrant genome organisation and decrease DNA damage respectively, in Hutchinson–Gilford progeria syndrome fibroblasts

    doi: 10.1007/s10522-018-9758-4

    Figure Lengend Snippet: Comparisons of the fraction of telomeres found in the residual nuclei of DNA Halo preparations of HGPS before and after drug treatments. Images of fixed control and HGPS DNA halos stained with both DAPI (blue) and Cy3 (red) telomeric PNA probes were captured at ×100 magnification. Residual nuclei were delineated and the fraction of telomeres determined inside and outside of the residual nuclei. Results for the treatments are shown as a 2DD with zoledronic acid, b FTI-277, c pravastatin, d zoledronic acid, e rapamycin, f N -acetyl- l -cysteine, g FTI-277 and GGTI-2133, h pravastatin and zoledronic acid and i FTI-277, pravastatin and zoledronic acid. The proportion of interior telomeres is expressed as a percentage of the total number of telomeres present. Error bars represent ± SEM. Scale bar = 10 µM

    Article Snippet: Performing FISH on these preparations, named HALO-FISH using telomere specific probes can delineate specific DNA sequences (Volpi and Bridger ; de Lange ).

    Techniques: Staining

    Alterations in size of residual nuclei in DNA Halo preparations of HGPS fibroblasts before and after drug treatments. Images of fixed HGPS DNA halos stained with DAPI taken at ×100 magnification were analysed by ImageJ delineating the residual nuclei and the entire DNA halo. The data displayed in this figure reveals the area measurement of the residual nuclei (x-axis). Results for the treatments are a FTI-277, b pravastatin, c zoledronic acid, d rapamycin, e IGF-1, f N -acetyl- l -cysteine, g FTI-277 and GGTI-2133, h pravastatin and zoledronic acid and i FTI-277, pravastatin and zoledronic acid. Error bars represent ± SEM

    Journal: Biogerontology

    Article Title: Farnesyltransferase inhibitor and rapamycin correct aberrant genome organisation and decrease DNA damage respectively, in Hutchinson–Gilford progeria syndrome fibroblasts

    doi: 10.1007/s10522-018-9758-4

    Figure Lengend Snippet: Alterations in size of residual nuclei in DNA Halo preparations of HGPS fibroblasts before and after drug treatments. Images of fixed HGPS DNA halos stained with DAPI taken at ×100 magnification were analysed by ImageJ delineating the residual nuclei and the entire DNA halo. The data displayed in this figure reveals the area measurement of the residual nuclei (x-axis). Results for the treatments are a FTI-277, b pravastatin, c zoledronic acid, d rapamycin, e IGF-1, f N -acetyl- l -cysteine, g FTI-277 and GGTI-2133, h pravastatin and zoledronic acid and i FTI-277, pravastatin and zoledronic acid. Error bars represent ± SEM

    Article Snippet: Performing FISH on these preparations, named HALO-FISH using telomere specific probes can delineate specific DNA sequences (Volpi and Bridger ; de Lange ).

    Techniques: Staining

    A range of images displaying characteristics of DNA halo preparations. Nucleoskeleton-associated DNA is anchored to the residual nucleus, while extracted, non-associated DNA forms the faint halo surrounding the structure. DNA has been stained using DAPI and can be seen in blue. Panel a displays a representative DNA Halo preparation that has been subjected to immuno-FISH using a painting probe for chromosome 17 (red) and the proliferation nucleolar marker pKi67 (green). Panel b displays another DNA Halo preparation with again the nucleolar proliferation marker pKi67 in green and CCND1 gene loci in red. Panel c is a DNA Halo preparation displaying a proliferating cell that has incorporated bromo-deoxy-uridine during DNA replication (red), DNA in blue and telomere sequences revealed with a PNA-telo probe in green. Panel d shows a residual nucleus surrounded by a halo of DNA visualised by feSEM. The morphology of the residual nucleus (RN) and surrounding DNA halo (DH) Magnification = 6 k scale bar = 2.5 µm. Panel e displays extracted, supercoiled DNA (white arrowheads) within the DNA halo, scale bar 300 nm. Panels f and g are representative control and HGPS fibroblasts with lamin A revealed by indirect immunofluorescence in unextracted cells, respectively and lamin A distribution present in residual nuclei of DNA halo preparations in extracted cells ( h and i ). Panels j – m display representative images of DNA halo preparations with telomeres in green in proliferating control and HGPS fibroblasts ( j and k , respectively) revealed with anti-Ki67 staining (red) and senescent control and HGPS fibroblasts ( l and m , respectively). Note in the HGPS cells telomere signals are located out with the residual nuclei. Scale bars in panels b and f = 10 μm

    Journal: Biogerontology

    Article Title: Farnesyltransferase inhibitor and rapamycin correct aberrant genome organisation and decrease DNA damage respectively, in Hutchinson–Gilford progeria syndrome fibroblasts

    doi: 10.1007/s10522-018-9758-4

    Figure Lengend Snippet: A range of images displaying characteristics of DNA halo preparations. Nucleoskeleton-associated DNA is anchored to the residual nucleus, while extracted, non-associated DNA forms the faint halo surrounding the structure. DNA has been stained using DAPI and can be seen in blue. Panel a displays a representative DNA Halo preparation that has been subjected to immuno-FISH using a painting probe for chromosome 17 (red) and the proliferation nucleolar marker pKi67 (green). Panel b displays another DNA Halo preparation with again the nucleolar proliferation marker pKi67 in green and CCND1 gene loci in red. Panel c is a DNA Halo preparation displaying a proliferating cell that has incorporated bromo-deoxy-uridine during DNA replication (red), DNA in blue and telomere sequences revealed with a PNA-telo probe in green. Panel d shows a residual nucleus surrounded by a halo of DNA visualised by feSEM. The morphology of the residual nucleus (RN) and surrounding DNA halo (DH) Magnification = 6 k scale bar = 2.5 µm. Panel e displays extracted, supercoiled DNA (white arrowheads) within the DNA halo, scale bar 300 nm. Panels f and g are representative control and HGPS fibroblasts with lamin A revealed by indirect immunofluorescence in unextracted cells, respectively and lamin A distribution present in residual nuclei of DNA halo preparations in extracted cells ( h and i ). Panels j – m display representative images of DNA halo preparations with telomeres in green in proliferating control and HGPS fibroblasts ( j and k , respectively) revealed with anti-Ki67 staining (red) and senescent control and HGPS fibroblasts ( l and m , respectively). Note in the HGPS cells telomere signals are located out with the residual nuclei. Scale bars in panels b and f = 10 μm

    Article Snippet: Performing FISH on these preparations, named HALO-FISH using telomere specific probes can delineate specific DNA sequences (Volpi and Bridger ; de Lange ).

    Techniques: Staining, Fluorescence In Situ Hybridization, Marker, Immunofluorescence