Structured Review

Sangon Biotech dna sequences
The real-time fluorescence curves of magnetic liposome-Im-LAMP assay reactions with different concentrations of P-gp:0 pg/ml (curve a), 160 pg/ml (curve b), 16 pg/ml (curve c), 1.6 pg/ml (curve d), 0.16 pg/ml (curve e), 1.6*10 −2 pg/ml (curve f). ( B ) The relationship between cycle time and log concentrations of P-gp. The results are average values of three repetitive measurements. ( C ) Specificity of magnetic liposome-Im-LAMP assay detection of P-gp: the real-time fluorescence curves of Im-LAMP reactions with different reference proteins, bovine serum albumin (BSA), carcinoembryonic antigen (CEA), hemoglobin (HGB), immunoglobulin G (IgG) and the blank. The Im-LAMP reactions were performed in a volume of 10 µL. <t>[FIP]</t> = [BIP] = 1.6 µM, [B3] = [F3] = 0.2 µM, [Bst <t>DNA</t> polymerase] = 8 U/µL.
Dna Sequences, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 93/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 23 article reviews
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Images

1) Product Images from "Magnetic-Immuno-Loop-Mediated Isothermal Amplification Based on DNA Encapsulating Liposome for the Ultrasensitive Detection of P-glycoprotein"

Article Title: Magnetic-Immuno-Loop-Mediated Isothermal Amplification Based on DNA Encapsulating Liposome for the Ultrasensitive Detection of P-glycoprotein

Journal: Scientific Reports

doi: 10.1038/s41598-017-10133-3

The real-time fluorescence curves of magnetic liposome-Im-LAMP assay reactions with different concentrations of P-gp:0 pg/ml (curve a), 160 pg/ml (curve b), 16 pg/ml (curve c), 1.6 pg/ml (curve d), 0.16 pg/ml (curve e), 1.6*10 −2 pg/ml (curve f). ( B ) The relationship between cycle time and log concentrations of P-gp. The results are average values of three repetitive measurements. ( C ) Specificity of magnetic liposome-Im-LAMP assay detection of P-gp: the real-time fluorescence curves of Im-LAMP reactions with different reference proteins, bovine serum albumin (BSA), carcinoembryonic antigen (CEA), hemoglobin (HGB), immunoglobulin G (IgG) and the blank. The Im-LAMP reactions were performed in a volume of 10 µL. [FIP] = [BIP] = 1.6 µM, [B3] = [F3] = 0.2 µM, [Bst DNA polymerase] = 8 U/µL.
Figure Legend Snippet: The real-time fluorescence curves of magnetic liposome-Im-LAMP assay reactions with different concentrations of P-gp:0 pg/ml (curve a), 160 pg/ml (curve b), 16 pg/ml (curve c), 1.6 pg/ml (curve d), 0.16 pg/ml (curve e), 1.6*10 −2 pg/ml (curve f). ( B ) The relationship between cycle time and log concentrations of P-gp. The results are average values of three repetitive measurements. ( C ) Specificity of magnetic liposome-Im-LAMP assay detection of P-gp: the real-time fluorescence curves of Im-LAMP reactions with different reference proteins, bovine serum albumin (BSA), carcinoembryonic antigen (CEA), hemoglobin (HGB), immunoglobulin G (IgG) and the blank. The Im-LAMP reactions were performed in a volume of 10 µL. [FIP] = [BIP] = 1.6 µM, [B3] = [F3] = 0.2 µM, [Bst DNA polymerase] = 8 U/µL.

Techniques Used: Fluorescence, Lamp Assay

2) Product Images from "Template-directed Chemical Ligation to Obtain 3?-3? and 5?-5? Phosphodiester DNA Linkages"

Article Title: Template-directed Chemical Ligation to Obtain 3?-3? and 5?-5? Phosphodiester DNA Linkages

Journal: Scientific Reports

doi: 10.1038/srep04595

Ligation reactions catalyzed by different mechanisms. (A) Normal 5′-3′ ligation catalyzed by DNA ligase. At the presence of template, ATP and T4 DNA ligase, two oligonucleotides were ligated together and a 5′-3′ phosphodiester bonds was formed. (B) Template-directed chemical ligation of 3′-3′ and 5′-5′ oligonucleotides activated by the coupling reagent N -Cyanoimidazole. Arrows in red represent the parallel oligonucleotide with template.
Figure Legend Snippet: Ligation reactions catalyzed by different mechanisms. (A) Normal 5′-3′ ligation catalyzed by DNA ligase. At the presence of template, ATP and T4 DNA ligase, two oligonucleotides were ligated together and a 5′-3′ phosphodiester bonds was formed. (B) Template-directed chemical ligation of 3′-3′ and 5′-5′ oligonucleotides activated by the coupling reagent N -Cyanoimidazole. Arrows in red represent the parallel oligonucleotide with template.

Techniques Used: Ligation

Related Articles

High Performance Liquid Chromatography:

Article Title: Magnetic-Immuno-Loop-Mediated Isothermal Amplification Based on DNA Encapsulating Liposome for the Ultrasensitive Detection of P-glycoprotein
Article Snippet: .. The DNA sequences: FIP, BIP, B3, F3, and the template DNA (Table in supporting information) used in this study were synthesized and purified with high-performance liquid chromatography (HPLC) by Sangon Biotechnology Co. Ltd. (Shanghai, People’s Republic of China). .. The process of the LAMP amplification was monitored by a real-time fluorescence PCR system (BIOER LineGene 9640).

Nucleic Acid Electrophoresis:

Article Title: Association of the GALNT2 gene polymorphisms and several environmental factors with serum lipid levels in the Mulao and Han populations
Article Snippet: .. The PCR products were purified by low melting point gel electrophoresis and phenol extraction, and then the DNA sequences were analyzed by using an ABI Prism 3100 (Applied Biosyatems) in Shanghai Sangon Biological Engineering Technology & Services Co., Ltd., People's Republic of China. .. Diagnostic criteria The normal values of serum TC, TG, HDL-C, LDL-C, ApoA1, ApoB levels and the ratio of ApoA1 to ApoB in our Clinical Science Experiment Center were 3.10-5.17, 0.56-1.70, 1.16-1.42, 2.70-3.10 mmol/L, 1.20-1.60, 0.80-1.05 g/L, and 1.00-2.50; respectively.

Article Title: Association of ATP binding cassette transporter G8 rs4148217 SNP and serum lipid levels in Mulao and Han nationalities
Article Snippet: .. The PCR products were purified by low melting point gel electrophoresis and phenol extraction, and then the DNA sequences were analyzed in Shanghai Sangon Biological Engineering Technology & Services Co., Ltd., People’s Republic of China. .. Diagnostic criteria The normal values of serum TC, TG, HDL-C, LDL-C, ApoA1, ApoB levels and the ratio of ApoA1 to ApoB in our Clinical Science Experiment Center were 3.10-5.17, 0.56-1.70, 1.16-1.42, 2.70-3.10 mmol/L, 1.20-1.60, 0.80-1.05 g/L, and 1.00-2.50; respectively.

Synthesized:

Article Title: Magnetic-Immuno-Loop-Mediated Isothermal Amplification Based on DNA Encapsulating Liposome for the Ultrasensitive Detection of P-glycoprotein
Article Snippet: .. The DNA sequences: FIP, BIP, B3, F3, and the template DNA (Table in supporting information) used in this study were synthesized and purified with high-performance liquid chromatography (HPLC) by Sangon Biotechnology Co. Ltd. (Shanghai, People’s Republic of China). .. The process of the LAMP amplification was monitored by a real-time fluorescence PCR system (BIOER LineGene 9640).

Article Title: Antiviral activity of porcine interferon omega 7 against foot‐and‐mouth disease virus in vitro, et al. Antiviral activity of porcine interferon omega 7 against foot‐and‐mouth disease virus in vitro
Article Snippet: .. 2.2 Plasmid construction The DNA sequence of mature PoIFN‐ω7 (Gene accession EU797621) was synthesized by Shanghai Sangon Biotechnology Co, Ltd (Shanghai, China). .. After digested by Eco RI and Hin d III restriction sites from the vector, the target gene was ligated into the corresponding sites in the pET30a vector (Invitrogen, CA).

Purification:

Article Title: Association of the GALNT2 gene polymorphisms and several environmental factors with serum lipid levels in the Mulao and Han populations
Article Snippet: .. The PCR products were purified by low melting point gel electrophoresis and phenol extraction, and then the DNA sequences were analyzed by using an ABI Prism 3100 (Applied Biosyatems) in Shanghai Sangon Biological Engineering Technology & Services Co., Ltd., People's Republic of China. .. Diagnostic criteria The normal values of serum TC, TG, HDL-C, LDL-C, ApoA1, ApoB levels and the ratio of ApoA1 to ApoB in our Clinical Science Experiment Center were 3.10-5.17, 0.56-1.70, 1.16-1.42, 2.70-3.10 mmol/L, 1.20-1.60, 0.80-1.05 g/L, and 1.00-2.50; respectively.

Article Title: Magnetic-Immuno-Loop-Mediated Isothermal Amplification Based on DNA Encapsulating Liposome for the Ultrasensitive Detection of P-glycoprotein
Article Snippet: .. The DNA sequences: FIP, BIP, B3, F3, and the template DNA (Table in supporting information) used in this study were synthesized and purified with high-performance liquid chromatography (HPLC) by Sangon Biotechnology Co. Ltd. (Shanghai, People’s Republic of China). .. The process of the LAMP amplification was monitored by a real-time fluorescence PCR system (BIOER LineGene 9640).

Article Title: Association of ATP binding cassette transporter G8 rs4148217 SNP and serum lipid levels in Mulao and Han nationalities
Article Snippet: .. The PCR products were purified by low melting point gel electrophoresis and phenol extraction, and then the DNA sequences were analyzed in Shanghai Sangon Biological Engineering Technology & Services Co., Ltd., People’s Republic of China. .. Diagnostic criteria The normal values of serum TC, TG, HDL-C, LDL-C, ApoA1, ApoB levels and the ratio of ApoA1 to ApoB in our Clinical Science Experiment Center were 3.10-5.17, 0.56-1.70, 1.16-1.42, 2.70-3.10 mmol/L, 1.20-1.60, 0.80-1.05 g/L, and 1.00-2.50; respectively.

Produced:

Article Title: Long non-coding RNA HOTAIR enhances radioresistance in MDA-MB231 breast cancer cells
Article Snippet: .. The RNA sequences targeting human HOTAIR were designed according to a previous study ( ) and produced by Sangon Biotech Co., Ltd. (Shanghai, China). .. The sequences used to construct the primers were: HOTAIR sense, 5′-ATAGGCAAATGTCAGAGGGTT-3′ and antisense, 5′-ATTCTTAAATTGGGCTGGGTC-3′; and β-actin sense, 5′-AAAGACCTGTACGCCAACAC-3′ and antisense, 5′-GTCATACTCCTGCTTGCTGAT-3′.

Polymerase Chain Reaction:

Article Title: Association of the GALNT2 gene polymorphisms and several environmental factors with serum lipid levels in the Mulao and Han populations
Article Snippet: .. The PCR products were purified by low melting point gel electrophoresis and phenol extraction, and then the DNA sequences were analyzed by using an ABI Prism 3100 (Applied Biosyatems) in Shanghai Sangon Biological Engineering Technology & Services Co., Ltd., People's Republic of China. .. Diagnostic criteria The normal values of serum TC, TG, HDL-C, LDL-C, ApoA1, ApoB levels and the ratio of ApoA1 to ApoB in our Clinical Science Experiment Center were 3.10-5.17, 0.56-1.70, 1.16-1.42, 2.70-3.10 mmol/L, 1.20-1.60, 0.80-1.05 g/L, and 1.00-2.50; respectively.

Article Title: Association of ATP binding cassette transporter G8 rs4148217 SNP and serum lipid levels in Mulao and Han nationalities
Article Snippet: .. The PCR products were purified by low melting point gel electrophoresis and phenol extraction, and then the DNA sequences were analyzed in Shanghai Sangon Biological Engineering Technology & Services Co., Ltd., People’s Republic of China. .. Diagnostic criteria The normal values of serum TC, TG, HDL-C, LDL-C, ApoA1, ApoB levels and the ratio of ApoA1 to ApoB in our Clinical Science Experiment Center were 3.10-5.17, 0.56-1.70, 1.16-1.42, 2.70-3.10 mmol/L, 1.20-1.60, 0.80-1.05 g/L, and 1.00-2.50; respectively.

Sequencing:

Article Title: Antiviral activity of porcine interferon omega 7 against foot‐and‐mouth disease virus in vitro, et al. Antiviral activity of porcine interferon omega 7 against foot‐and‐mouth disease virus in vitro
Article Snippet: .. 2.2 Plasmid construction The DNA sequence of mature PoIFN‐ω7 (Gene accession EU797621) was synthesized by Shanghai Sangon Biotechnology Co, Ltd (Shanghai, China). .. After digested by Eco RI and Hin d III restriction sites from the vector, the target gene was ligated into the corresponding sites in the pET30a vector (Invitrogen, CA).

Plasmid Preparation:

Article Title: Antiviral activity of porcine interferon omega 7 against foot‐and‐mouth disease virus in vitro, et al. Antiviral activity of porcine interferon omega 7 against foot‐and‐mouth disease virus in vitro
Article Snippet: .. 2.2 Plasmid construction The DNA sequence of mature PoIFN‐ω7 (Gene accession EU797621) was synthesized by Shanghai Sangon Biotechnology Co, Ltd (Shanghai, China). .. After digested by Eco RI and Hin d III restriction sites from the vector, the target gene was ligated into the corresponding sites in the pET30a vector (Invitrogen, CA).

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    Sangon Biotech mutant 3 utr dna sequences
    Fluorescence values of 293T cells transfected with wild-type or mutant 3′-untranslated region <t>DNA</t> sequences of MMP1 and antagomiR-222. Dual luciferase reporter assay was used to assess the interaction between miR-222 and MMP1. Data are presented as the mean ± standard deviation. **P
    Mutant 3 Utr Dna Sequences, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 91/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mutant 3 utr dna sequences/product/Sangon Biotech
    Average 91 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    mutant 3 utr dna sequences - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    93
    Sangon Biotech dna sequence analysis
    Detection of the Sj28S gene component in the different parasite developmental stages of S. japonicum in O. hupensis by the <t>LAMP</t> method. 1. Negative control (nuclease-free water); 2. Positive control (Sj28 plasmid <t>DNA);</t> 3. Cercariae; 4. Specificity control ( Trichobilharzia cercariae); 5. Negative control (pooled snail DNA from a non-endemic area); 6. Mother sporocyst; 7. Daughter sporocyst.
    Dna Sequence Analysis, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna sequence analysis/product/Sangon Biotech
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    dna sequence analysis - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    Fluorescence values of 293T cells transfected with wild-type or mutant 3′-untranslated region DNA sequences of MMP1 and antagomiR-222. Dual luciferase reporter assay was used to assess the interaction between miR-222 and MMP1. Data are presented as the mean ± standard deviation. **P

    Journal: Experimental and Therapeutic Medicine

    Article Title: MicroRNA-222 regulates the viability of fibroblasts in hypertrophic scars via matrix metalloproteinase 1

    doi: 10.3892/etm.2017.5634

    Figure Lengend Snippet: Fluorescence values of 293T cells transfected with wild-type or mutant 3′-untranslated region DNA sequences of MMP1 and antagomiR-222. Dual luciferase reporter assay was used to assess the interaction between miR-222 and MMP1. Data are presented as the mean ± standard deviation. **P

    Article Snippet: Plasmids (0.8 µg) with WT or mutant 3′-UTR DNA sequences were co-transfected with antagomiR-222 (100 nM; Sangon Biotech, Shanghai, China) into 293T cells (Cell Bank of the Chinese Academy of Sciences, Shanghai, China).

    Techniques: Fluorescence, Transfection, Mutagenesis, Luciferase, Reporter Assay, Standard Deviation

    Fluorescence values of HEK293T cells transfected with WT or mutant 3′-untranslated region DNA sequences of importin-α3 and miR-24 mimic. A dual luciferase reporter assay was used to evaluate the interaction between miR-24 and importin-α3. *P

    Journal: Experimental and Therapeutic Medicine

    Article Title: MicroRNA-24 inhibits the proliferation and migration of endothelial cells in patients with atherosclerosis by targeting importin-α3 and regulating inflammatory responses

    doi: 10.3892/etm.2017.5355

    Figure Lengend Snippet: Fluorescence values of HEK293T cells transfected with WT or mutant 3′-untranslated region DNA sequences of importin-α3 and miR-24 mimic. A dual luciferase reporter assay was used to evaluate the interaction between miR-24 and importin-α3. *P

    Article Snippet: Plasmids (0.5 µg) with WT or mutant 3′-UTR DNA sequences were co-transfected with miR-24 mimic (100 nM; Sangon Biotech Co., Ltd., Shanghai, China) into HEK293T cells (ATCC, Manassas, VA, USA).

    Techniques: Fluorescence, Transfection, Mutagenesis, Luciferase, Reporter Assay

    Fluorescence values of HUVECs transfected with WT or mutant 3′-UTR DNA sequences of BECN1 as well as miR-506-3p. A dual-luciferase reporter assay was used to evaluate the interaction between miR-506-3p and BECN1. *P

    Journal: Experimental and Therapeutic Medicine

    Article Title: Overexpression of microRNA-506-3p aggravates the injury of vascular endothelial cells in patients with hypertension by downregulating Beclin1 expression

    doi: 10.3892/etm.2018.5733

    Figure Lengend Snippet: Fluorescence values of HUVECs transfected with WT or mutant 3′-UTR DNA sequences of BECN1 as well as miR-506-3p. A dual-luciferase reporter assay was used to evaluate the interaction between miR-506-3p and BECN1. *P

    Article Snippet: Plasmids (1 µg) with WT or mutant 3′-UTR DNA sequences were co-transfected with miR-506-3p mimics (100 nM; Sangon Biotech, Shanghai, China) into HEK293T cells.

    Techniques: Fluorescence, Transfection, Mutagenesis, Luciferase, Reporter Assay

    Detection of the Sj28S gene component in the different parasite developmental stages of S. japonicum in O. hupensis by the LAMP method. 1. Negative control (nuclease-free water); 2. Positive control (Sj28 plasmid DNA); 3. Cercariae; 4. Specificity control ( Trichobilharzia cercariae); 5. Negative control (pooled snail DNA from a non-endemic area); 6. Mother sporocyst; 7. Daughter sporocyst.

    Journal: Tropical Medicine and Infectious Disease

    Article Title: Field Evaluation of a Loop-Mediated Isothermal Amplification (LAMP) Platform for the Detection of Schistosoma japonicum Infection in Oncomelania hupensis Snails

    doi: 10.3390/tropicalmed3040124

    Figure Lengend Snippet: Detection of the Sj28S gene component in the different parasite developmental stages of S. japonicum in O. hupensis by the LAMP method. 1. Negative control (nuclease-free water); 2. Positive control (Sj28 plasmid DNA); 3. Cercariae; 4. Specificity control ( Trichobilharzia cercariae); 5. Negative control (pooled snail DNA from a non-endemic area); 6. Mother sporocyst; 7. Daughter sporocyst.

    Article Snippet: Out of the 232 pooled snail samples tested, 217 were found to be negative and 14 positive by both assays , while the remaining single sample, found positive by LAMP but negative by PCR, was found to be positive by DNA sequence analysis, underlining LAMP’s superiority.

    Techniques: Negative Control, Positive Control, Plasmid Preparation