dna polymerases  (Solis BioDyne)

Journal: BMC Genomics

Article Title: Silhouette scores for assessment of SNP genotype clusters

doi: 10.1186/1471-2164-6-35

Figure Lengend Snippet: Examples of Silhouette scores. Examples of genotype clusters from nine SNP assays, each with the results from 16 samples genotyped in duplicate using Tag-array minisequencing with the calculated Silhouette scores shown in the right hand upper corner of each panel. The blue circles represent homozygotes for allele 2, the red triangles are heterozygotes and the green squares are homozygotes for allele 1. The SNPs are denoted by their dbSNP identification number, and the DNA polarities analyzed are indicated by "cod" or "nc".

Article Snippet: The DNA polymerases were; TERMIPol (Solis BioDyne, Tartu, Estonia), Therminator (New England BioLabs Inc., Beverly, MA, USA), KlenThermase (Gene Craft, Lüdinghausen, Germany), or ThermoSequenase (Amersham Biosciences, Uppsala, Sweden).

Techniques:

Journal: BMC Genomics

Article Title: Silhouette scores for assessment of SNP genotype clusters

doi: 10.1186/1471-2164-6-35

Figure Lengend Snippet: Distribution of Silhouette scores from minisequencing assays using four DNA polymerases. The Silhouette score is given on the y-axis. Each black diamond represents the Silhouette score for one SNP assay. The light blue rectangular boxes indicate those 75% of the scatter plots that yielded the highest silhouette scores for each enzyme. Quartiles are indicated by the black horizontal lines.

Article Snippet: The DNA polymerases were; TERMIPol (Solis BioDyne, Tartu, Estonia), Therminator (New England BioLabs Inc., Beverly, MA, USA), KlenThermase (Gene Craft, Lüdinghausen, Germany), or ThermoSequenase (Amersham Biosciences, Uppsala, Sweden).

Techniques:

Journal: Iranian Journal of Parasitology

Article Title: Frequency of Toxoplasma gondii in HIV Positive Patients from West of Iran by ELISA and PCR

doi:

Figure Lengend Snippet: Result of PCR from HIV/AIDS positive sera with T. gondii antigenemia from Sanandaj, Kordistan, west of Iran in 2011 Lane M: marker (Solis – Biodyne)/ Lanes 1 and 2: HIV- positive serum with T. gondii antigenemia, stained with etidium bromid and DNA safe stain, respectively./ Lane 3: Positive control (DNA of tachyzoites of T. gondii )/ Lanes 4 and 5: negative control serum.

Article Snippet: After an initial denaturation at 95°C for 10 min, 40 cycles were run, including denaturation (92°C for 30 sec), annealing (55°C for 50 sec), and extension (72°C for 30 sec) and final extension at 72°C for 7 min. PCR products and DNA ladder (Solis_Biodyne, Estonia) were electrophoresed in 1.5% agarose gel (Merck, Germany) and stained with ethidium bromide and DNA safestain.

Techniques: Polymerase Chain Reaction, Marker, Staining, Positive Control, Negative Control

Journal: International Journal of Molecular Sciences

Article Title: In-Depth Sequence Analysis of Bread Wheat VRN1 Genes

doi: 10.3390/ijms222212284

Figure Lengend Snippet: The secondary structure of the vrn - A1 promoter may prevent successful amplification and sequencing. ( a ) Schematic representation of vrn - A1 promoter variants with 137 bp, 181 bp and 194 bp deletions found in winter cultivars and the position of the G-quadruplex (G4). ( b ) Sequence motif of G4 found in Triple Dirk C (TDC, MH347747). ( c ) DNA fold prediction of G4 found in TDC.

Article Snippet: Long amplicons (from 6 to 11 kb) were amplified by PrimeSTAR GXL DNA Polymerase (Takara Bio, Kusatsu, Japan) and Expand Long Range, dNTPack (Roche, Basel, Switzerland), and short amplicons (from 600 bp to 3 kb) were amplified by HOT FIREPol DNA Polymerase (Solis BioDyne, Tartu, Estonia), all according to the manufacturer’s instructions.

Techniques: Amplification, Sequencing

Journal: Microorganisms

Article Title: Reliable and Sensitive Nested PCR for the Detection of Chlamydia in Sputum

doi: 10.3390/microorganisms9050935

Figure Lengend Snippet: Threshold value of the NPCR assay for C. pneumoniae detection in sputa. Lines: M λ/ Pst I size marker; 500 µL of negative sputum was spiked with 1 µL of 10 −4 –10 1 dilution of original culture C. pneumoniae TWAR-183; DNA was isolated as described in the Materials and Methods section, and 0.5 µL was added to the first PCR reaction, amplified by external primers, and 1 µL was amplified in the second reaction; 10 −4 –10 1 serial dilutions; NC negative controls. ( A ) PNEU S/PNEU Nok; Chp up/Chp down primers. Size of PCR product is 461 bp. ( B ) PNEU S/Short down; Short up/a Chtinok primers. Size of PCR product is 121 bp.

Article Snippet: The DNA was amplified by FIREpolDNA polymerase FIREPol® DNA Polymerase (Solis BioDyne, Tartu, Estonia) in a 25 μL solution containing 1× buffer B, 0.2 mM dNTPs, 2.5 mM MgCl2, 1× S solution, 1 pmol/μL of each primer, 0.5 μL of DNA, and 0.04 U/μL of enzyme (461-bp amplicon).

Techniques: Marker, Isolation, Polymerase Chain Reaction, Amplification