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TaKaRa dna polymerase
Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 247 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dna polymerase/product/TaKaRa
Average 99 stars, based on 247 article reviews
Price from $9.99 to $1999.99
dna polymerase - by Bioz Stars, 2020-02
99/100 stars

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DNA Extraction:

Article Title: Effect of curcumin on vascular endothelial growth factor in hypoxic HepG2 cells via the insulin-like growth factor 1 receptor signaling pathway
Article Snippet: After cell cultures were 70% confluent, they were harvested and the genomic DNA was extracted using DNA Extraction kits (Genloci Biotechnologies, Inc.). .. DNA polymerase (cat. no. R060Q; Takara, Bio, Inc., Otsu, Japan) was use for PCR.

Clone Assay:

Article Title: Two novel cross-protective antigens for bovine Pasteurella multocida
Article Snippet: DNApol was from Takara Biotechnology Co., Ltd. (Chongqing, China) and the primers for the selected genes are presented in . .. The products of polymerase chain reaction (94°C for 3 min, 94°C for 1 min, 50°C for 1 min, 72°C for 1 min, 35 cycles at 72°C for 10 min, stored at 4°C) were cloned into pET-28a plasmid (Takara Biotechnology Co., Ltd.) in E.coli DH5α (Takara Biotechnology Co., Ltd.), expressed in E. coli BL21 (DE3; Takara Biotechnology Co., Ltd.), cultured at 37°C in LB broth or on agar supplemented with 100 µg/ml kanamycin (Sigma-Aldrich; Merck KGaA) and induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (Sigma-Aldrich; Merck KGaA) at 30°C or 37°C for 4 h. The recombinant proteins were purified using affinity chromatography and Ni-NTA Superflow cartridges (Qiagen GmbH, Hilden, Germany).

Amplification:

Article Title: Two novel cross-protective antigens for bovine Pasteurella multocida
Article Snippet: The target genes were amplified from the genomic DNA of strain PmCQ2. .. DNApol was from Takara Biotechnology Co., Ltd. (Chongqing, China) and the primers for the selected genes are presented in .

Electroporation:

Article Title: Effect of curcumin on vascular endothelial growth factor in hypoxic HepG2 cells via the insulin-like growth factor 1 receptor signaling pathway
Article Snippet: HepG2 cells were seeded in a 24-well plate 24 h prior to transfection and transfected with the pGK1.1-U6-IGF-1RgRNA plasmid via the electroporation method using a Neon® Transfection system (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. .. DNA polymerase (cat. no. R060Q; Takara, Bio, Inc., Otsu, Japan) was use for PCR.

Size-exclusion Chromatography:

Article Title: Effect of curcumin on vascular endothelial growth factor in hypoxic HepG2 cells via the insulin-like growth factor 1 receptor signaling pathway
Article Snippet: DNA polymerase (cat. no. R060Q; Takara, Bio, Inc., Otsu, Japan) was use for PCR. .. PCR was performed using the following thermocycling conditions: 95°C for 10 sec, 60°C for 10 sec, 72°C for 20 sec for 30 cycles.

Cell Culture:

Article Title: Two novel cross-protective antigens for bovine Pasteurella multocida
Article Snippet: DNApol was from Takara Biotechnology Co., Ltd. (Chongqing, China) and the primers for the selected genes are presented in . .. The products of polymerase chain reaction (94°C for 3 min, 94°C for 1 min, 50°C for 1 min, 72°C for 1 min, 35 cycles at 72°C for 10 min, stored at 4°C) were cloned into pET-28a plasmid (Takara Biotechnology Co., Ltd.) in E.coli DH5α (Takara Biotechnology Co., Ltd.), expressed in E. coli BL21 (DE3; Takara Biotechnology Co., Ltd.), cultured at 37°C in LB broth or on agar supplemented with 100 µg/ml kanamycin (Sigma-Aldrich; Merck KGaA) and induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (Sigma-Aldrich; Merck KGaA) at 30°C or 37°C for 4 h. The recombinant proteins were purified using affinity chromatography and Ni-NTA Superflow cartridges (Qiagen GmbH, Hilden, Germany).

Purification:

Article Title: Two novel cross-protective antigens for bovine Pasteurella multocida
Article Snippet: Paragraph title: Protein expression and purification ... DNApol was from Takara Biotechnology Co., Ltd. (Chongqing, China) and the primers for the selected genes are presented in .

Plasmid Preparation:

Article Title: Two novel cross-protective antigens for bovine Pasteurella multocida
Article Snippet: DNApol was from Takara Biotechnology Co., Ltd. (Chongqing, China) and the primers for the selected genes are presented in . .. The products of polymerase chain reaction (94°C for 3 min, 94°C for 1 min, 50°C for 1 min, 72°C for 1 min, 35 cycles at 72°C for 10 min, stored at 4°C) were cloned into pET-28a plasmid (Takara Biotechnology Co., Ltd.) in E.coli DH5α (Takara Biotechnology Co., Ltd.), expressed in E. coli BL21 (DE3; Takara Biotechnology Co., Ltd.), cultured at 37°C in LB broth or on agar supplemented with 100 µg/ml kanamycin (Sigma-Aldrich; Merck KGaA) and induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (Sigma-Aldrich; Merck KGaA) at 30°C or 37°C for 4 h. The recombinant proteins were purified using affinity chromatography and Ni-NTA Superflow cartridges (Qiagen GmbH, Hilden, Germany).

Article Title: Effect of curcumin on vascular endothelial growth factor in hypoxic HepG2 cells via the insulin-like growth factor 1 receptor signaling pathway
Article Snippet: HepG2 cells were seeded in a 24-well plate 24 h prior to transfection and transfected with the pGK1.1-U6-IGF-1RgRNA plasmid via the electroporation method using a Neon® Transfection system (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. .. DNA polymerase (cat. no. R060Q; Takara, Bio, Inc., Otsu, Japan) was use for PCR.

Polymerase Chain Reaction:

Article Title: Two novel cross-protective antigens for bovine Pasteurella multocida
Article Snippet: DNApol was from Takara Biotechnology Co., Ltd. (Chongqing, China) and the primers for the selected genes are presented in . .. The products of polymerase chain reaction (94°C for 3 min, 94°C for 1 min, 50°C for 1 min, 72°C for 1 min, 35 cycles at 72°C for 10 min, stored at 4°C) were cloned into pET-28a plasmid (Takara Biotechnology Co., Ltd.) in E.coli DH5α (Takara Biotechnology Co., Ltd.), expressed in E. coli BL21 (DE3; Takara Biotechnology Co., Ltd.), cultured at 37°C in LB broth or on agar supplemented with 100 µg/ml kanamycin (Sigma-Aldrich; Merck KGaA) and induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (Sigma-Aldrich; Merck KGaA) at 30°C or 37°C for 4 h. The recombinant proteins were purified using affinity chromatography and Ni-NTA Superflow cartridges (Qiagen GmbH, Hilden, Germany).

Article Title: Effect of curcumin on vascular endothelial growth factor in hypoxic HepG2 cells via the insulin-like growth factor 1 receptor signaling pathway
Article Snippet: .. DNA polymerase (cat. no. R060Q; Takara, Bio, Inc., Otsu, Japan) was use for PCR. ..

Affinity Chromatography:

Article Title: Two novel cross-protective antigens for bovine Pasteurella multocida
Article Snippet: DNApol was from Takara Biotechnology Co., Ltd. (Chongqing, China) and the primers for the selected genes are presented in . .. The products of polymerase chain reaction (94°C for 3 min, 94°C for 1 min, 50°C for 1 min, 72°C for 1 min, 35 cycles at 72°C for 10 min, stored at 4°C) were cloned into pET-28a plasmid (Takara Biotechnology Co., Ltd.) in E.coli DH5α (Takara Biotechnology Co., Ltd.), expressed in E. coli BL21 (DE3; Takara Biotechnology Co., Ltd.), cultured at 37°C in LB broth or on agar supplemented with 100 µg/ml kanamycin (Sigma-Aldrich; Merck KGaA) and induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (Sigma-Aldrich; Merck KGaA) at 30°C or 37°C for 4 h. The recombinant proteins were purified using affinity chromatography and Ni-NTA Superflow cartridges (Qiagen GmbH, Hilden, Germany).

Knock-Out:

Article Title: Effect of curcumin on vascular endothelial growth factor in hypoxic HepG2 cells via the insulin-like growth factor 1 receptor signaling pathway
Article Snippet: Paragraph title: Preparation of IGF-1R knockout cells ... DNA polymerase (cat. no. R060Q; Takara, Bio, Inc., Otsu, Japan) was use for PCR.

Expressing:

Article Title: Two novel cross-protective antigens for bovine Pasteurella multocida
Article Snippet: Paragraph title: Protein expression and purification ... DNApol was from Takara Biotechnology Co., Ltd. (Chongqing, China) and the primers for the selected genes are presented in .

Sequencing:

Article Title: Effect of curcumin on vascular endothelial growth factor in hypoxic HepG2 cells via the insulin-like growth factor 1 receptor signaling pathway
Article Snippet: DNA polymerase (cat. no. R060Q; Takara, Bio, Inc., Otsu, Japan) was use for PCR. .. Amplicons were sent to the Beijing Genomics Institute (Beijing, China) for deep sequencing.

Recombinant:

Article Title: Two novel cross-protective antigens for bovine Pasteurella multocida
Article Snippet: DNApol was from Takara Biotechnology Co., Ltd. (Chongqing, China) and the primers for the selected genes are presented in . .. The products of polymerase chain reaction (94°C for 3 min, 94°C for 1 min, 50°C for 1 min, 72°C for 1 min, 35 cycles at 72°C for 10 min, stored at 4°C) were cloned into pET-28a plasmid (Takara Biotechnology Co., Ltd.) in E.coli DH5α (Takara Biotechnology Co., Ltd.), expressed in E. coli BL21 (DE3; Takara Biotechnology Co., Ltd.), cultured at 37°C in LB broth or on agar supplemented with 100 µg/ml kanamycin (Sigma-Aldrich; Merck KGaA) and induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (Sigma-Aldrich; Merck KGaA) at 30°C or 37°C for 4 h. The recombinant proteins were purified using affinity chromatography and Ni-NTA Superflow cartridges (Qiagen GmbH, Hilden, Germany).

Positron Emission Tomography:

Article Title: Two novel cross-protective antigens for bovine Pasteurella multocida
Article Snippet: DNApol was from Takara Biotechnology Co., Ltd. (Chongqing, China) and the primers for the selected genes are presented in . .. The products of polymerase chain reaction (94°C for 3 min, 94°C for 1 min, 50°C for 1 min, 72°C for 1 min, 35 cycles at 72°C for 10 min, stored at 4°C) were cloned into pET-28a plasmid (Takara Biotechnology Co., Ltd.) in E.coli DH5α (Takara Biotechnology Co., Ltd.), expressed in E. coli BL21 (DE3; Takara Biotechnology Co., Ltd.), cultured at 37°C in LB broth or on agar supplemented with 100 µg/ml kanamycin (Sigma-Aldrich; Merck KGaA) and induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (Sigma-Aldrich; Merck KGaA) at 30°C or 37°C for 4 h. The recombinant proteins were purified using affinity chromatography and Ni-NTA Superflow cartridges (Qiagen GmbH, Hilden, Germany).

High Performance Liquid Chromatography:

Article Title: Conditional control of RNA-guided nucleic acid cleavage and gene editing
Article Snippet: Pyrobest™ DNA Polymerase and PrimeSTAR HS DNA Polymerase were purchased from TaKaRa Shuzo Co. Ltd. (Tokyo, Japan). .. The oligonucleotides at HPLC purity were obtained from TaKaRa company (Dalian, China).

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  • 90
    TaKaRa hs prime start dna polymerase
    Gallate and protocatechuate decarboxylase activity in lactic acid bacteria. (A and B) <t>PCR</t> amplification of the B and C subunits of putative gallate decarboxylases. Chromosomal <t>DNA</t> from the following strains was used for PCR amplification with oligonucleotides
    Hs Prime Start Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hs prime start dna polymerase/product/TaKaRa
    Average 90 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    hs prime start dna polymerase - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    90
    TaKaRa hot start dna polymerase
    GPX3 expression and methylation in THP1 cell line before and after 5-aza-dC treatment. A: GPX3 relative expression levels. B: Electrophoresis results of <t>RQ-PCR</t> and RQ-MSP products. 1: Gene Ruler TM 100 bp <t>DNA</t> ladder; 2: 0 μM; 3: 0.1 μM;
    Hot Start Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hot start dna polymerase/product/TaKaRa
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hot start dna polymerase - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    90
    TaKaRa t4 dna polymerase
    The PCR product of a foreign gene was amplified by <t>T4</t> DNA polymerase and dGTP, and then was ligated with the Bsu36I-digested pRTRA. The ligation mixture was transformed to the donor strain DH10β, and then the recombinant donor plasmid was obtained. We introduced the two different Bsu36I sites (CCTTAGG and CCTGAGG) in the pRTRA vector and the 4 nt TTAC(5′–3′) in the forward primer and the other 4 nt TGAC(5′–3′) in the reverse primer. The complete digestion of pRTRA with Bsu36I results in a linearized donor vector with overhang ends of 5′-TTA-3′ and 5′-TCA-3′, respectively. We made use of the 3′→5′ exonuclease activity and 5′→3′ polymerase activity of T4 DNA polymerase. When T4 DNA polymerase encounters the first Guanine nucleotide at the 5′ end of the DNA in the dGTP bath, the reaction will keep the balance between the exonuclease activity and polymerase activity. Therefore, the overhang ends of the gene fragments of interest will be digested to be perfectly compatible with the vector.
    T4 Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna polymerase/product/TaKaRa
    Average 90 stars, based on 59 article reviews
    Price from $9.99 to $1999.99
    t4 dna polymerase - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    Image Search Results


    Gallate and protocatechuate decarboxylase activity in lactic acid bacteria. (A and B) PCR amplification of the B and C subunits of putative gallate decarboxylases. Chromosomal DNA from the following strains was used for PCR amplification with oligonucleotides

    Journal: Applied and Environmental Microbiology

    Article Title: Uncovering the Lactobacillus plantarum WCFS1 Gallate Decarboxylase Involved in Tannin Degradation

    doi: 10.1128/AEM.00840-13

    Figure Lengend Snippet: Gallate and protocatechuate decarboxylase activity in lactic acid bacteria. (A and B) PCR amplification of the B and C subunits of putative gallate decarboxylases. Chromosomal DNA from the following strains was used for PCR amplification with oligonucleotides

    Article Snippet: The lpdB , lpdC , and lpdD genes from L. plantarum WCFS1 were PCR amplified by using HS Prime Start DNA polymerase (TaKaRa) and primer pairs 455 and 390 ( lpdB ), 454 and 388 ( lpdC ), and 1141 and 1142 ( lpdD ).

    Techniques: Activity Assay, Polymerase Chain Reaction, Amplification

    GPX3 expression and methylation in THP1 cell line before and after 5-aza-dC treatment. A: GPX3 relative expression levels. B: Electrophoresis results of RQ-PCR and RQ-MSP products. 1: Gene Ruler TM 100 bp DNA ladder; 2: 0 μM; 3: 0.1 μM;

    Journal: American Journal of Cancer Research

    Article Title: GPX3 hypermethylation serves as an independent prognostic biomarker in non-M3 acute myeloid leukemia

    doi:

    Figure Lengend Snippet: GPX3 expression and methylation in THP1 cell line before and after 5-aza-dC treatment. A: GPX3 relative expression levels. B: Electrophoresis results of RQ-PCR and RQ-MSP products. 1: Gene Ruler TM 100 bp DNA ladder; 2: 0 μM; 3: 0.1 μM;

    Article Snippet: Bisulfite sequencing PCR (BSP) reaction system contained 1 × PCR buffer (KCl 0.25 mM), dNTP Mixture 6.25 μM, primers 0.5 μM, hot start DNA polymerase 0.75 U (Takara, Tokyo, Japan), and modified DNA 20 ng.

    Techniques: Expressing, Methylation, Electrophoresis, Polymerase Chain Reaction

    Electrophoresis results of RQ-PCR and RQ-MSP products in normal controls and AML patients. 1: Gene Ruler TM 100 bp DNA ladder; 2, 3: controls; 4-7: AML patients; 8: positive control; 9: negative control. A: GPX3 expression; B: GPX3 methylation; C: GPX3

    Journal: American Journal of Cancer Research

    Article Title: GPX3 hypermethylation serves as an independent prognostic biomarker in non-M3 acute myeloid leukemia

    doi:

    Figure Lengend Snippet: Electrophoresis results of RQ-PCR and RQ-MSP products in normal controls and AML patients. 1: Gene Ruler TM 100 bp DNA ladder; 2, 3: controls; 4-7: AML patients; 8: positive control; 9: negative control. A: GPX3 expression; B: GPX3 methylation; C: GPX3

    Article Snippet: Bisulfite sequencing PCR (BSP) reaction system contained 1 × PCR buffer (KCl 0.25 mM), dNTP Mixture 6.25 μM, primers 0.5 μM, hot start DNA polymerase 0.75 U (Takara, Tokyo, Japan), and modified DNA 20 ng.

    Techniques: Electrophoresis, Polymerase Chain Reaction, Positive Control, Negative Control, Expressing, Methylation

    The PCR product of a foreign gene was amplified by T4 DNA polymerase and dGTP, and then was ligated with the Bsu36I-digested pRTRA. The ligation mixture was transformed to the donor strain DH10β, and then the recombinant donor plasmid was obtained. We introduced the two different Bsu36I sites (CCTTAGG and CCTGAGG) in the pRTRA vector and the 4 nt TTAC(5′–3′) in the forward primer and the other 4 nt TGAC(5′–3′) in the reverse primer. The complete digestion of pRTRA with Bsu36I results in a linearized donor vector with overhang ends of 5′-TTA-3′ and 5′-TCA-3′, respectively. We made use of the 3′→5′ exonuclease activity and 5′→3′ polymerase activity of T4 DNA polymerase. When T4 DNA polymerase encounters the first Guanine nucleotide at the 5′ end of the DNA in the dGTP bath, the reaction will keep the balance between the exonuclease activity and polymerase activity. Therefore, the overhang ends of the gene fragments of interest will be digested to be perfectly compatible with the vector.

    Journal: Nucleic Acids Research

    Article Title: A novel and simple method for construction of recombinant adenoviruses

    doi: 10.1093/nar/gkl449

    Figure Lengend Snippet: The PCR product of a foreign gene was amplified by T4 DNA polymerase and dGTP, and then was ligated with the Bsu36I-digested pRTRA. The ligation mixture was transformed to the donor strain DH10β, and then the recombinant donor plasmid was obtained. We introduced the two different Bsu36I sites (CCTTAGG and CCTGAGG) in the pRTRA vector and the 4 nt TTAC(5′–3′) in the forward primer and the other 4 nt TGAC(5′–3′) in the reverse primer. The complete digestion of pRTRA with Bsu36I results in a linearized donor vector with overhang ends of 5′-TTA-3′ and 5′-TCA-3′, respectively. We made use of the 3′→5′ exonuclease activity and 5′→3′ polymerase activity of T4 DNA polymerase. When T4 DNA polymerase encounters the first Guanine nucleotide at the 5′ end of the DNA in the dGTP bath, the reaction will keep the balance between the exonuclease activity and polymerase activity. Therefore, the overhang ends of the gene fragments of interest will be digested to be perfectly compatible with the vector.

    Article Snippet: Cloning the foreign genes gfp and man into the donor plasmid using restriction enzyme Bsu36I and T4 DNA polymerase The gfp gene was amplified from pEGFP-1 (Clontech) by PCR.

    Techniques: Polymerase Chain Reaction, Amplification, Ligation, Transformation Assay, Recombinant, Plasmid Preparation, Activity Assay

    Schematic overview of the construction of a DNA-shuffled and truncated enzyme library by the MURA method. The sequences of the MURA primer can be chosen on any desired site of the parent gene. This figure represents a procedure using a 3′-complementary MURA primer, and thus N-terminal-truncated and shuffled variants of PlaA are constructed. The steps shown are random fragmentation of the parent gene pool by DNase I (a), unidirectional reassembly with the MURA primer (b), separation of the fragments of interest by preparative agarose gel electrophoresis (c), formation of blunt ends by S1 nuclease or T4 DNA polymerase on both termini (d), sticky-end formation by a restriction enzyme on one terminus (e), and ligation into an expression vector (f). The order of steps c, d, and e can be altered to d, e, and c without affecting the efficiency of library construction. The diamonds on the bars representing genes indicate possible mutations during shuffled and unidirectional reassembly PCR.

    Journal: Applied and Environmental Microbiology

    Article Title: Construction of DNA-Shuffled and Incrementally Truncated Libraries by a Mutagenic and Unidirectional Reassembly Method: Changing from a Substrate Specificity of Phospholipase to That of Lipase

    doi: 10.1128/AEM.68.12.6146-6151.2002

    Figure Lengend Snippet: Schematic overview of the construction of a DNA-shuffled and truncated enzyme library by the MURA method. The sequences of the MURA primer can be chosen on any desired site of the parent gene. This figure represents a procedure using a 3′-complementary MURA primer, and thus N-terminal-truncated and shuffled variants of PlaA are constructed. The steps shown are random fragmentation of the parent gene pool by DNase I (a), unidirectional reassembly with the MURA primer (b), separation of the fragments of interest by preparative agarose gel electrophoresis (c), formation of blunt ends by S1 nuclease or T4 DNA polymerase on both termini (d), sticky-end formation by a restriction enzyme on one terminus (e), and ligation into an expression vector (f). The order of steps c, d, and e can be altered to d, e, and c without affecting the efficiency of library construction. The diamonds on the bars representing genes indicate possible mutations during shuffled and unidirectional reassembly PCR.

    Article Snippet: After the unidirectionally reassembled DNA fragments were purified, they were blunt-ended on both ends with T4 DNA polymerase or S1 nuclease and then sticky-ended on the 3′ end with Sal I. Fragments in the range of about 500 to 960 bp were isolated by preparative agarose gel electrophoresis and cloned into pSTV28.

    Techniques: Construct, Agarose Gel Electrophoresis, Ligation, Expressing, Plasmid Preparation, Polymerase Chain Reaction