dna polymerase  (Qiagen)

 
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    Name:
    Taq DNA Polymerase
    Description:
    Qiagen Taq DNA Polymerase 250U 5U L 10 min at 97C 60 min at 94C Half life 2 to 4 kb min at 72C Extension Rate Genomic DNA and cDNA Sample PCR Amplification Exonuclease Enzyme Activity Minimal Optimization Ideal for Standard and Specialized PCR Applications Includes Taq DNA Polymerase 10x PCR Buffer 10x CoralLoad PCR Buffer 5x Q Solution 25mM MgCl2
    Catalog Number:
    201203
    Price:
    134
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    Structured Review

    Qiagen dna polymerase
    Sensitivity limits of multiplex RT-PCR. Lane 1 Amplification from tenfold diluted cDNA; lanes 2–6 amplicons from 10 −2 to 10 −6 fold diluted cDNA. Lanes 1–6 amplicons obtained with multiplexing kit; lanes 7–12 amplification with hot-start <t>Taq</t> <t>DNA</t> polymerase at same dilutions; lane M 100 bp marker
    Qiagen Taq DNA Polymerase 250U 5U L 10 min at 97C 60 min at 94C Half life 2 to 4 kb min at 72C Extension Rate Genomic DNA and cDNA Sample PCR Amplification Exonuclease Enzyme Activity Minimal Optimization Ideal for Standard and Specialized PCR Applications Includes Taq DNA Polymerase 10x PCR Buffer 10x CoralLoad PCR Buffer 5x Q Solution 25mM MgCl2
    https://www.bioz.com/result/dna polymerase/product/Qiagen
    Average 99 stars, based on 75 article reviews
    Price from $9.99 to $1999.99
    dna polymerase - by Bioz Stars, 2020-07
    99/100 stars

    Images

    1) Product Images from "Simultaneous Detection of Major Pome Fruit Viruses and a Viroid"

    Article Title: Simultaneous Detection of Major Pome Fruit Viruses and a Viroid

    Journal: Indian Journal of Microbiology

    doi: 10.1007/s12088-013-0431-y

    Sensitivity limits of multiplex RT-PCR. Lane 1 Amplification from tenfold diluted cDNA; lanes 2–6 amplicons from 10 −2 to 10 −6 fold diluted cDNA. Lanes 1–6 amplicons obtained with multiplexing kit; lanes 7–12 amplification with hot-start Taq DNA polymerase at same dilutions; lane M 100 bp marker
    Figure Legend Snippet: Sensitivity limits of multiplex RT-PCR. Lane 1 Amplification from tenfold diluted cDNA; lanes 2–6 amplicons from 10 −2 to 10 −6 fold diluted cDNA. Lanes 1–6 amplicons obtained with multiplexing kit; lanes 7–12 amplification with hot-start Taq DNA polymerase at same dilutions; lane M 100 bp marker

    Techniques Used: Multiplex Assay, Reverse Transcription Polymerase Chain Reaction, Amplification, Multiplexing, Marker

    2) Product Images from "Improvements of rolling circle amplification (RCA) efficiency and accuracy using Thermus thermophilus SSB mutant protein"

    Article Title: Improvements of rolling circle amplification (RCA) efficiency and accuracy using Thermus thermophilus SSB mutant protein

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkl350

    PCR examinations of RCA amplified whole genome. PCR amplifications were carried out on 0.5 µl samples of RCA products using human genomic DNA as the template. The products were electrophoresed and stained with ethidium bromide. ( a ) A diagrammatic representation of the subdivided region (a-1–a-6, 8077 bp in GenBank Acc. No. X91835 ). Nucleotide (nt) numbers correspond to registries in GenBank. The PCR amplifications using the RCA products from the reaction shown in Figure 5a lane 1 ( b ), Figure 5a lane 3 ( c ), Figure 5c lane 1 ( d ) and Figure 5c lane 3 ( e ). Throughout (b–e), the six subdivided sites are indicated as lanes 1–6. Locations of the specific PCR products are indicated by arrows.
    Figure Legend Snippet: PCR examinations of RCA amplified whole genome. PCR amplifications were carried out on 0.5 µl samples of RCA products using human genomic DNA as the template. The products were electrophoresed and stained with ethidium bromide. ( a ) A diagrammatic representation of the subdivided region (a-1–a-6, 8077 bp in GenBank Acc. No. X91835 ). Nucleotide (nt) numbers correspond to registries in GenBank. The PCR amplifications using the RCA products from the reaction shown in Figure 5a lane 1 ( b ), Figure 5a lane 3 ( c ), Figure 5c lane 1 ( d ) and Figure 5c lane 3 ( e ). Throughout (b–e), the six subdivided sites are indicated as lanes 1–6. Locations of the specific PCR products are indicated by arrows.

    Techniques Used: Polymerase Chain Reaction, Amplification, Staining

    Multiplex PCR examinations of RCA amplified whole genome. ( a ) Control, multiplex PCR amplifications for the 12 randomly selected human genes using human genomic DNA as the template. Amount of the template DNA for the PCR is indicated. Multiplex PCR amplifications of the 12 human genes using RCA products as the template in the absence ( b ) or presence ( c ) of Tth SSB-255 protein. Amount of the template DNA for RCA is serially diluted as indicated. Throughout (a–c), all samples were amplified with primers at the same primer pair concentrations (0.1 µM per pair). Aliquots of 5 µl volume were electrophoresed through 12.5% acrylamide gel in Tris–borate/EDTA buffer, and stained with SYBR Green (SYBR Green I, Novagen). The signals were detected using a Fluoro Imager (Fluoro Imager 595, Molecular Dynamics). Product sizes (from 57 to 360 bp) are indicated on the right or left side of each panel. The oligonucleotide sequences used for the primers are as follows. Primer sets 1 (chromosome 11), 5′-GGGCA GAGCC ATCTA TTGCT TACA-3′, 5′-GGTTG CTAGT GAACA CAGTT GTGTC A-3′; Primer sets 2 (chromosome 16), 5′-GCACT CTTCT GGTCC CCACA GA, 5′-TTGGT CTTGT CGGCA GGAGA CA-3′; Primer sets 3 (chromosome 8), 5′-GTCCT TCCCC CGCTG GAAAC-3′, 5′-GCAGC AGAGA TCATC GCGCC-3′; Primer sets 4 (chromosome 7), 5′-CACAG ATTTC CAAGG ATGCG CTG, 5′-CGTGC TCTGT TCCAG ACTTG-3′; Primer sets 5 (chromosome 10), 5′-CGTCT GGCGA TTGCT CCAAA TG-3′, 5′-GGGCA GTTGT GATCC ATGAG AA-3′; Primer sets 6 (chromosome 17), 5′-GCCTC TGATT CCTCA CTGAT TGCTC T, 5′-TGTCA ACCAC CCTTA ACCCC TCC-3′; Primer sets 7 (chromosome 20), 5′-TTGGA GGGGT GGGTG AGTCA AG, 5′-GGAGG GGTGG GGGTT AATGG TTA-3′; Primer sets 8 (chromosome 13), 5′-GGAAC AAGAC ACGGC TGGGT T-3′, 5′-AGCAA GGCAG GGCAG GCAAG T-3′; Primer sets 9 (chromosome 3), 5′-CGGTC CCATT CTCAG GGAAT CT-3′, 5′-GCCCA GAGGA AGAAG AAGGA AA-3′; Primer sets 10 (chromosome 1), 5′-GCCCC CACCC AGGTT GGTTT CTA-3′, 5′-ATGCC TTCAT CTGGC TCAGT GAA-3′; Primer sets 11 (chromosome 6), 5′-GCTCA GCATG GTGGT GGCAT AA-3′, 5′-CCTCA TACCT TCCCC CCCAT TT-3′; Primer sets 12 (chromosome 22), 5′-GACTA CTCTA GCGAC TGTCC ATCTC-3′, 5′-GACAG CCACC AGATC CAATC-3′.
    Figure Legend Snippet: Multiplex PCR examinations of RCA amplified whole genome. ( a ) Control, multiplex PCR amplifications for the 12 randomly selected human genes using human genomic DNA as the template. Amount of the template DNA for the PCR is indicated. Multiplex PCR amplifications of the 12 human genes using RCA products as the template in the absence ( b ) or presence ( c ) of Tth SSB-255 protein. Amount of the template DNA for RCA is serially diluted as indicated. Throughout (a–c), all samples were amplified with primers at the same primer pair concentrations (0.1 µM per pair). Aliquots of 5 µl volume were electrophoresed through 12.5% acrylamide gel in Tris–borate/EDTA buffer, and stained with SYBR Green (SYBR Green I, Novagen). The signals were detected using a Fluoro Imager (Fluoro Imager 595, Molecular Dynamics). Product sizes (from 57 to 360 bp) are indicated on the right or left side of each panel. The oligonucleotide sequences used for the primers are as follows. Primer sets 1 (chromosome 11), 5′-GGGCA GAGCC ATCTA TTGCT TACA-3′, 5′-GGTTG CTAGT GAACA CAGTT GTGTC A-3′; Primer sets 2 (chromosome 16), 5′-GCACT CTTCT GGTCC CCACA GA, 5′-TTGGT CTTGT CGGCA GGAGA CA-3′; Primer sets 3 (chromosome 8), 5′-GTCCT TCCCC CGCTG GAAAC-3′, 5′-GCAGC AGAGA TCATC GCGCC-3′; Primer sets 4 (chromosome 7), 5′-CACAG ATTTC CAAGG ATGCG CTG, 5′-CGTGC TCTGT TCCAG ACTTG-3′; Primer sets 5 (chromosome 10), 5′-CGTCT GGCGA TTGCT CCAAA TG-3′, 5′-GGGCA GTTGT GATCC ATGAG AA-3′; Primer sets 6 (chromosome 17), 5′-GCCTC TGATT CCTCA CTGAT TGCTC T, 5′-TGTCA ACCAC CCTTA ACCCC TCC-3′; Primer sets 7 (chromosome 20), 5′-TTGGA GGGGT GGGTG AGTCA AG, 5′-GGAGG GGTGG GGGTT AATGG TTA-3′; Primer sets 8 (chromosome 13), 5′-GGAAC AAGAC ACGGC TGGGT T-3′, 5′-AGCAA GGCAG GGCAG GCAAG T-3′; Primer sets 9 (chromosome 3), 5′-CGGTC CCATT CTCAG GGAAT CT-3′, 5′-GCCCA GAGGA AGAAG AAGGA AA-3′; Primer sets 10 (chromosome 1), 5′-GCCCC CACCC AGGTT GGTTT CTA-3′, 5′-ATGCC TTCAT CTGGC TCAGT GAA-3′; Primer sets 11 (chromosome 6), 5′-GCTCA GCATG GTGGT GGCAT AA-3′, 5′-CCTCA TACCT TCCCC CCCAT TT-3′; Primer sets 12 (chromosome 22), 5′-GACTA CTCTA GCGAC TGTCC ATCTC-3′, 5′-GACAG CCACC AGATC CAATC-3′.

    Techniques Used: Multiplex Assay, Polymerase Chain Reaction, Amplification, Acrylamide Gel Assay, Staining, SYBR Green Assay, CTG Assay

    3) Product Images from "TALENs Mediate Efficient and Heritable Mutation of Endogenous Genes in the Marine Annelid Platynereis dumerilii"

    Article Title: TALENs Mediate Efficient and Heritable Mutation of Endogenous Genes in the Marine Annelid Platynereis dumerilii

    Journal: Genetics

    doi: 10.1534/genetics.113.161091

    TALEN-induced mutations in the Platynereis estrogen receptor . (A) Schematic of the er locus showing target exons 2 and 3 with TALEN target sites (yellow). Blue arrows, primer positions; red double-ended arrow, region of sequence deleted in E; green, DNA-binding domain. Primer combinations used for screening are shown above in B–E. (B–E) PCR, undigested PCR product; NI, non-injected. (B) Restriction digest screening of larvae injected with er Ex3_L2/R2 TALENs (mRNA concentration: 267 ng/µl/TALEN mRNA). Arrowhead indicates uncut PCR product following AflI II digestion (asterisk). (C) Mutation evidence at exon 2 site: uncut band adult worm +3 vs. fully digested product from mutation-negative (−) TALEN-injected worm. (D) Adult worms er+31 and er+37 with mutations at exon 3 site. (E) Deletions (red arrow) detected in larvae and adult worms resulting from simultaneous cleavage at exons 2 and 3 using 300 ng/µl/TALEN mRNA: deletion positive (+); deletion negative (−). Please note different primer pairs used for larval vs. adult samples. (F) Mutant sequences obtained from digest screening for exons 1, 2, and long deletions. Numbers in brackets indicate the sample or worm from which the sequence was obtained; all other sequences are from injected larvae shown in B. Length of mutations are indicated by ∆ with “−” indicating deletions and “+” indicating insertions. Restriction site is shown in boldface type; asterisks indicate frameshift mutations. Shading key: yellow, TALEN binding sites; gray, spacer; blue, nucleotides differing from wild type; green, inserted nucleotides.
    Figure Legend Snippet: TALEN-induced mutations in the Platynereis estrogen receptor . (A) Schematic of the er locus showing target exons 2 and 3 with TALEN target sites (yellow). Blue arrows, primer positions; red double-ended arrow, region of sequence deleted in E; green, DNA-binding domain. Primer combinations used for screening are shown above in B–E. (B–E) PCR, undigested PCR product; NI, non-injected. (B) Restriction digest screening of larvae injected with er Ex3_L2/R2 TALENs (mRNA concentration: 267 ng/µl/TALEN mRNA). Arrowhead indicates uncut PCR product following AflI II digestion (asterisk). (C) Mutation evidence at exon 2 site: uncut band adult worm +3 vs. fully digested product from mutation-negative (−) TALEN-injected worm. (D) Adult worms er+31 and er+37 with mutations at exon 3 site. (E) Deletions (red arrow) detected in larvae and adult worms resulting from simultaneous cleavage at exons 2 and 3 using 300 ng/µl/TALEN mRNA: deletion positive (+); deletion negative (−). Please note different primer pairs used for larval vs. adult samples. (F) Mutant sequences obtained from digest screening for exons 1, 2, and long deletions. Numbers in brackets indicate the sample or worm from which the sequence was obtained; all other sequences are from injected larvae shown in B. Length of mutations are indicated by ∆ with “−” indicating deletions and “+” indicating insertions. Restriction site is shown in boldface type; asterisks indicate frameshift mutations. Shading key: yellow, TALEN binding sites; gray, spacer; blue, nucleotides differing from wild type; green, inserted nucleotides.

    Techniques Used: Sequencing, Binding Assay, Polymerase Chain Reaction, Injection, TALENs, Concentration Assay, Mutagenesis

    4) Product Images from "Molecular detection of a novel cyprinid herpesvirus in roach (Rutilus rutilus) and asp (Leuciscus aspius) showing typical signs of carp pox disease"

    Article Title: Molecular detection of a novel cyprinid herpesvirus in roach (Rutilus rutilus) and asp (Leuciscus aspius) showing typical signs of carp pox disease

    Journal: Archives of Virology

    doi: 10.1007/s00705-020-04638-y

    (a) Midpoint-rooted phylogenetic tree for alloherpesviruses. The analysis was based on Bayesian analysis (WAG amino acid [aa] substitution model) and the maximum-likelihood (ML) method (RTRev aa substitution model) of the partial DNA polymerase sequences (79 amino acids characters). Both methods produced the same tree topology. The main lineages (genera) within the family are indicated by different coloured lines on the tree. (b) Phylogeny reconstruction for the genus Cyprinivirus inferred by Bayesian analysis and ML (JTT aa model for both) using the concatenated amino acid sequences of DNA polymerase and terminase genes (411 amino acids). Both methods produced the same tree topology. AciHV-2 was selected as the outgroup. High statistical values confirm the topology of the trees. Posterior probability values are shown in bold characters, while the bootstrap values for ML are in italics. (c) Tanglegram of host-virus coevolution within the genus Cyprinivirus . Congruence was observed between the phylogenetic relationships among CyHVs after ML and Bayesian inference of the concatenated sequences of the DNA polymerase and terminase genes (right) and host species after ML and Bayesian inference of mitochondrial sequences (1138 nucleotides from the cytochrome-b gene) (left). Abbreviations: AciHV, acipenserid herpesvirus; AngHV, anguillid herpesvirus; CyHV, cyprinid herpesvirus; GaHV, gadid herpesvirus; IcHV, ictalurid herpesvirus; RaHV, ranid herpesvirus; SalHV, salmonid herpesvirus
    Figure Legend Snippet: (a) Midpoint-rooted phylogenetic tree for alloherpesviruses. The analysis was based on Bayesian analysis (WAG amino acid [aa] substitution model) and the maximum-likelihood (ML) method (RTRev aa substitution model) of the partial DNA polymerase sequences (79 amino acids characters). Both methods produced the same tree topology. The main lineages (genera) within the family are indicated by different coloured lines on the tree. (b) Phylogeny reconstruction for the genus Cyprinivirus inferred by Bayesian analysis and ML (JTT aa model for both) using the concatenated amino acid sequences of DNA polymerase and terminase genes (411 amino acids). Both methods produced the same tree topology. AciHV-2 was selected as the outgroup. High statistical values confirm the topology of the trees. Posterior probability values are shown in bold characters, while the bootstrap values for ML are in italics. (c) Tanglegram of host-virus coevolution within the genus Cyprinivirus . Congruence was observed between the phylogenetic relationships among CyHVs after ML and Bayesian inference of the concatenated sequences of the DNA polymerase and terminase genes (right) and host species after ML and Bayesian inference of mitochondrial sequences (1138 nucleotides from the cytochrome-b gene) (left). Abbreviations: AciHV, acipenserid herpesvirus; AngHV, anguillid herpesvirus; CyHV, cyprinid herpesvirus; GaHV, gadid herpesvirus; IcHV, ictalurid herpesvirus; RaHV, ranid herpesvirus; SalHV, salmonid herpesvirus

    Techniques Used: Produced

    5) Product Images from "Simultaneous Detection of Major Pome Fruit Viruses and a Viroid"

    Article Title: Simultaneous Detection of Major Pome Fruit Viruses and a Viroid

    Journal: Indian Journal of Microbiology

    doi: 10.1007/s12088-013-0431-y

    Sensitivity limits of multiplex RT-PCR. Lane 1 Amplification from tenfold diluted cDNA; lanes 2–6 amplicons from 10 −2 to 10 −6 fold diluted cDNA. Lanes 1–6 amplicons obtained with multiplexing kit; lanes 7–12 amplification with hot-start Taq DNA polymerase at same dilutions; lane M 100 bp marker
    Figure Legend Snippet: Sensitivity limits of multiplex RT-PCR. Lane 1 Amplification from tenfold diluted cDNA; lanes 2–6 amplicons from 10 −2 to 10 −6 fold diluted cDNA. Lanes 1–6 amplicons obtained with multiplexing kit; lanes 7–12 amplification with hot-start Taq DNA polymerase at same dilutions; lane M 100 bp marker

    Techniques Used: Multiplex Assay, Reverse Transcription Polymerase Chain Reaction, Amplification, Multiplexing, Marker

    Related Articles

    Multiplexing:

    Article Title: Simultaneous Detection of Major Pome Fruit Viruses and a Viroid
    Article Snippet: .. The multiplex RT-PCR assay was optimized with regular Taq (Bangalore Genei, Bengaluru, India), Hot Start Taq (Fermentas, Vilnus, Lithuania) DNA polymerase and multiplexing kit from Qiagen under the conditions described above with nad5 as an internal control. .. Leaf samples were tested by double-antibody sandwich ELISA (DAS-ELISA) for the targeted viruses ACLSV, ApMV, ASPV, and ASGV using a commercial kit (Bioreba, Reinach, Switzerland).

    Multiplex Assay:

    Article Title: Simultaneous Detection of Major Pome Fruit Viruses and a Viroid
    Article Snippet: .. The multiplex RT-PCR assay was optimized with regular Taq (Bangalore Genei, Bengaluru, India), Hot Start Taq (Fermentas, Vilnus, Lithuania) DNA polymerase and multiplexing kit from Qiagen under the conditions described above with nad5 as an internal control. .. Leaf samples were tested by double-antibody sandwich ELISA (DAS-ELISA) for the targeted viruses ACLSV, ApMV, ASPV, and ASGV using a commercial kit (Bioreba, Reinach, Switzerland).

    Article Title: Improvements of rolling circle amplification (RCA) efficiency and accuracy using Thermus thermophilus SSB mutant protein
    Article Snippet: .. PCR was performed using DNA polymerase from Thermus aquaticus (Taq ) according to the manufacturer's instructions (QIAGEN HotSartTaq DNA polymerase kit and QIAGEN Multiplex PCR kit, QIAGEN). .. PCR thermal cycles used were 15 min at 95°C for DNA denaturation, followed by 35 cycles of 30 s at 94°C, 90 s at 60°C and 90 s at 72°C.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Simultaneous Detection of Major Pome Fruit Viruses and a Viroid
    Article Snippet: .. The multiplex RT-PCR assay was optimized with regular Taq (Bangalore Genei, Bengaluru, India), Hot Start Taq (Fermentas, Vilnus, Lithuania) DNA polymerase and multiplexing kit from Qiagen under the conditions described above with nad5 as an internal control. .. Leaf samples were tested by double-antibody sandwich ELISA (DAS-ELISA) for the targeted viruses ACLSV, ApMV, ASPV, and ASGV using a commercial kit (Bioreba, Reinach, Switzerland).

    Southern Blot:

    Article Title: Transgenically Expressed T-Rep of Tomato Yellow Leaf Curl Sardinia Virus Acts as a trans-Dominant-Negative Mutant, Inhibiting Viral Transcription and Replication
    Article Snippet: .. To verify the nature of these slowly migrating molecules, TNAs extracted from the samples at 24 and 72 h were incubated with Taq DNA polymerase and analyzed by Southern blot (Fig. B). ..

    Concentration Assay:

    Article Title: Detection of knockdown resistance (kdr) mutations in Anopheles gambiae: a comparison of two new high-throughput assays with existing methods
    Article Snippet: .. PCR was carried out in a 25 μl volume with a final concentration of 1 × Buffer, 2 mM MgCl2 , 0.2 mM dNTPs, 0.1 μM each primer, 0.034 U/μl Taq DNA polymerase (Qiagen). ..

    Incubation:

    Article Title: Transgenically Expressed T-Rep of Tomato Yellow Leaf Curl Sardinia Virus Acts as a trans-Dominant-Negative Mutant, Inhibiting Viral Transcription and Replication
    Article Snippet: .. To verify the nature of these slowly migrating molecules, TNAs extracted from the samples at 24 and 72 h were incubated with Taq DNA polymerase and analyzed by Southern blot (Fig. B). ..

    other:

    Article Title: Transgenically Expressed T-Rep of Tomato Yellow Leaf Curl Sardinia Virus Acts as a trans-Dominant-Negative Mutant, Inhibiting Viral Transcription and Replication
    Article Snippet: TNAs of wt protoplasts cotransfected with pTOM6 together with pTOM100C4(−) or pTOM100NT were treated with Taq DNA polymerase for increasing times (Fig. A).

    Article Title: Transgenically Expressed T-Rep of Tomato Yellow Leaf Curl Sardinia Virus Acts as a trans-Dominant-Negative Mutant, Inhibiting Viral Transcription and Replication
    Article Snippet: A fraction of viral DNA from pTOM6-pTOM100NT-cotransfected protoplasts also reacted with Taq DNA polymerase, generating ocDNA.

    Polymerase Chain Reaction:

    Article Title: Improvements of rolling circle amplification (RCA) efficiency and accuracy using Thermus thermophilus SSB mutant protein
    Article Snippet: .. PCR was performed using DNA polymerase from Thermus aquaticus (Taq ) according to the manufacturer's instructions (QIAGEN HotSartTaq DNA polymerase kit and QIAGEN Multiplex PCR kit, QIAGEN). .. PCR thermal cycles used were 15 min at 95°C for DNA denaturation, followed by 35 cycles of 30 s at 94°C, 90 s at 60°C and 90 s at 72°C.

    Article Title: Detection of knockdown resistance (kdr) mutations in Anopheles gambiae: a comparison of two new high-throughput assays with existing methods
    Article Snippet: .. PCR was carried out in a 25 μl volume with a final concentration of 1 × Buffer, 2 mM MgCl2 , 0.2 mM dNTPs, 0.1 μM each primer, 0.034 U/μl Taq DNA polymerase (Qiagen). ..

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  • 86
    Qiagen polymerase chain reaction pcr amplification dna
    Helicobacteraceae <t>DNA</t> was detected with Single <t>PCR</t> in all the colonic biopsies: Evolutionary relationships of taxa for helicobacter bilis the evolutionary history was inferred using the UPGMA, Kimura 2-parameter and MEGA5 methods.
    Polymerase Chain Reaction Pcr Amplification Dna, supplied by Qiagen, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polymerase chain reaction pcr amplification dna/product/Qiagen
    Average 86 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    polymerase chain reaction pcr amplification dna - by Bioz Stars, 2020-07
    86/100 stars
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    85
    Qiagen polymerase chain reaction high molecular weight genomic dna
    Detection of <t>HPV</t> infection by PCR in OSMF and OSCC cases . Gel figure A represent the ethidium bromide-staining in 2% agarose gel and showing presence of HPV infection in OSMF OSCC cases with an amplicon of L1 consensus (450 bp) and Gel figure B represent the amplicon of HPV 16 E6 (506 bp). PC is positive control <t>DNA,</t> NC is negative control DNA, Lanes 1 to 9 are DNA samples from OSMF and OSCC cases, M = 100 bp molecular weight marker.
    Polymerase Chain Reaction High Molecular Weight Genomic Dna, supplied by Qiagen, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polymerase chain reaction high molecular weight genomic dna/product/Qiagen
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polymerase chain reaction high molecular weight genomic dna - by Bioz Stars, 2020-07
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    85
    Qiagen rfhvmm dna polymerase sequence
    CODEHOP PCR amplification of a region of the <t>RFHVMm</t> vTS gene with a thermal gradient. PCR amplification of <t>DNA</t> from the retroperitoneal fibromatosis tumor from MmuYN91 was performed with CODEHOPs derived from two conserved TS motifs, RHFGA and DMGLB. A gradient of annealing temperatures ranging from 55 to 70 o C was used. The expected 284-bp fragment is indicated. Lane temperatures: 1, 70°C; 2, 68.9°C; 3, 67.1°C; 4, 64.3°C; 5, 60.5°C; 6, 57.9°C; 7, 56.1°C; 8, 55.0°C.
    Rfhvmm Dna Polymerase Sequence, supplied by Qiagen, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rfhvmm dna polymerase sequence/product/Qiagen
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rfhvmm dna polymerase sequence - by Bioz Stars, 2020-07
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    99
    Qiagen taq dna polymerase
    Slowly migrating DNAs are converted into ocDNA by <t>Taq</t> (A) or T4 (B) <t>DNA</t> polymerase treatment. The blots were hybridized with a C1-sense RNA probe. The positions of ocDNA, linear DNA (linDNA), scDNA, and cssDNA forms of viral DNA are indicated. Slowly migrating viral DNAs are indicated with an asterisk (∗). (A) TNAs were extracted from wt protoplasts at 72 h posttransfection with pTOM6 alone (the two lanes on the right) or together with pTOM100C4(−) or pTOM100NT and analyzed directly (−) or following incubation with Taq DNA polymerase for the time indicated below. (B) TNAs were extracted from wt or transgenic (102.22) protoplasts at 72 h posttransfection with pSP97 (TYLCSV-ES[1]) and analyzed following a 1-h incubation with (+) or without (−) T4 DNA polymerase. Lane C, TNAs from a TYLCSV-infected tomato plant digested with Bgl II to show migration of linear DNA.
    Taq Dna Polymerase, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq dna polymerase/product/Qiagen
    Average 99 stars, based on 86 article reviews
    Price from $9.99 to $1999.99
    taq dna polymerase - by Bioz Stars, 2020-07
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    Image Search Results


    Helicobacteraceae DNA was detected with Single PCR in all the colonic biopsies: Evolutionary relationships of taxa for helicobacter bilis the evolutionary history was inferred using the UPGMA, Kimura 2-parameter and MEGA5 methods.

    Journal: Diagnostic Pathology

    Article Title: Detection of Helicobacter spp. DNA in the colonic biopsies of stray dogs: molecular and histopathological investigations

    doi: 10.1186/1746-1596-9-50

    Figure Lengend Snippet: Helicobacteraceae DNA was detected with Single PCR in all the colonic biopsies: Evolutionary relationships of taxa for helicobacter bilis the evolutionary history was inferred using the UPGMA, Kimura 2-parameter and MEGA5 methods.

    Article Snippet: Polymerase chain reaction (PCR) amplification DNA was extracted from biopsies by using a DNA easy tissue KIT® (Qiagen, Hilden, Germany) according to the manufacturer’s Instructions.

    Techniques: Polymerase Chain Reaction

    Helicobacteraceae DNA was detected with single PCR in all the colonic biopsies: Evolutionary relationships of taxa for helicobacter canis the evolutionary history was inferred using the UPGMA, Kimura 2-parameter and MEGA5 methods.

    Journal: Diagnostic Pathology

    Article Title: Detection of Helicobacter spp. DNA in the colonic biopsies of stray dogs: molecular and histopathological investigations

    doi: 10.1186/1746-1596-9-50

    Figure Lengend Snippet: Helicobacteraceae DNA was detected with single PCR in all the colonic biopsies: Evolutionary relationships of taxa for helicobacter canis the evolutionary history was inferred using the UPGMA, Kimura 2-parameter and MEGA5 methods.

    Article Snippet: Polymerase chain reaction (PCR) amplification DNA was extracted from biopsies by using a DNA easy tissue KIT® (Qiagen, Hilden, Germany) according to the manufacturer’s Instructions.

    Techniques: Polymerase Chain Reaction

    Helicobacteraceae DNA was detected with single PCR in all the colonic biopsies: Evolutionary relationships of taxa for helicobacter salomonis the evolutionary history was inferred using the UPGMA, Kimura 2-parameter and MEGA5 methods.

    Journal: Diagnostic Pathology

    Article Title: Detection of Helicobacter spp. DNA in the colonic biopsies of stray dogs: molecular and histopathological investigations

    doi: 10.1186/1746-1596-9-50

    Figure Lengend Snippet: Helicobacteraceae DNA was detected with single PCR in all the colonic biopsies: Evolutionary relationships of taxa for helicobacter salomonis the evolutionary history was inferred using the UPGMA, Kimura 2-parameter and MEGA5 methods.

    Article Snippet: Polymerase chain reaction (PCR) amplification DNA was extracted from biopsies by using a DNA easy tissue KIT® (Qiagen, Hilden, Germany) according to the manufacturer’s Instructions.

    Techniques: Polymerase Chain Reaction

    The Polymerase Chain Reaction ( PCR ) on DNA isolated with standard protocol ; PCR amplification of DNA from bacterial strains by (NO.1-7) H.bilis P17f/P17r primer; (NO. 8-22) H.canis canis3F/canis4r primer; (NO.23-34) H.salomonis HSALF/HSALR primer; (NO.35-37) H.felis Fe1f/Fe3r primer; (NO.38-52) H.bizzozeronii Bi1F/Bi2R primer; (NO.53) H.pylori HP-for/HP-REV Primer, and 100-bp molecular ladder.

    Journal: Diagnostic Pathology

    Article Title: Detection of Helicobacter spp. DNA in the colonic biopsies of stray dogs: molecular and histopathological investigations

    doi: 10.1186/1746-1596-9-50

    Figure Lengend Snippet: The Polymerase Chain Reaction ( PCR ) on DNA isolated with standard protocol ; PCR amplification of DNA from bacterial strains by (NO.1-7) H.bilis P17f/P17r primer; (NO. 8-22) H.canis canis3F/canis4r primer; (NO.23-34) H.salomonis HSALF/HSALR primer; (NO.35-37) H.felis Fe1f/Fe3r primer; (NO.38-52) H.bizzozeronii Bi1F/Bi2R primer; (NO.53) H.pylori HP-for/HP-REV Primer, and 100-bp molecular ladder.

    Article Snippet: Polymerase chain reaction (PCR) amplification DNA was extracted from biopsies by using a DNA easy tissue KIT® (Qiagen, Hilden, Germany) according to the manufacturer’s Instructions.

    Techniques: Polymerase Chain Reaction, Isolation, Amplification

    Detection of HPV infection by PCR in OSMF and OSCC cases . Gel figure A represent the ethidium bromide-staining in 2% agarose gel and showing presence of HPV infection in OSMF OSCC cases with an amplicon of L1 consensus (450 bp) and Gel figure B represent the amplicon of HPV 16 E6 (506 bp). PC is positive control DNA, NC is negative control DNA, Lanes 1 to 9 are DNA samples from OSMF and OSCC cases, M = 100 bp molecular weight marker.

    Journal: Virology Journal

    Article Title: Comparative study between the Hybrid Capture II test and PCR based assay for the detection of human papillomavirus DNA in oral submucous fibrosis and oral squamous cell carcinoma

    doi: 10.1186/1743-422X-7-253

    Figure Lengend Snippet: Detection of HPV infection by PCR in OSMF and OSCC cases . Gel figure A represent the ethidium bromide-staining in 2% agarose gel and showing presence of HPV infection in OSMF OSCC cases with an amplicon of L1 consensus (450 bp) and Gel figure B represent the amplicon of HPV 16 E6 (506 bp). PC is positive control DNA, NC is negative control DNA, Lanes 1 to 9 are DNA samples from OSMF and OSCC cases, M = 100 bp molecular weight marker.

    Article Snippet: DNA extraction and diagnosis of high risk HPV infection by polymerase chain reaction High molecular weight genomic DNA was extracted from oral biopsies of patients by using the Qiagen QIAamp DNA tissue Kit (Qiagen Inc. USA) The extracted genomic DNA was quantified and checked for purity spectrophotometrically (Spectro UV-Vis Double Beam PC, UVD Model 2950 LABOMED, Inc. CA, USA) Ethidium bromide (EtBr) stained 0.8% agrose gel electrophoresis was used to confirm presence of DNA in samples.

    Techniques: Infection, Polymerase Chain Reaction, Staining, Agarose Gel Electrophoresis, Amplification, Positive Control, Negative Control, Molecular Weight, Marker

    Figure 1 illustrated the oral sub mucous fibrosis (OSMF) and oral squamous cell carcinoma (OSCC) specimen collection and HPV DNA testing methods .

    Journal: Virology Journal

    Article Title: Comparative study between the Hybrid Capture II test and PCR based assay for the detection of human papillomavirus DNA in oral submucous fibrosis and oral squamous cell carcinoma

    doi: 10.1186/1743-422X-7-253

    Figure Lengend Snippet: Figure 1 illustrated the oral sub mucous fibrosis (OSMF) and oral squamous cell carcinoma (OSCC) specimen collection and HPV DNA testing methods .

    Article Snippet: DNA extraction and diagnosis of high risk HPV infection by polymerase chain reaction High molecular weight genomic DNA was extracted from oral biopsies of patients by using the Qiagen QIAamp DNA tissue Kit (Qiagen Inc. USA) The extracted genomic DNA was quantified and checked for purity spectrophotometrically (Spectro UV-Vis Double Beam PC, UVD Model 2950 LABOMED, Inc. CA, USA) Ethidium bromide (EtBr) stained 0.8% agrose gel electrophoresis was used to confirm presence of DNA in samples.

    Techniques:

    CODEHOP PCR amplification of a region of the RFHVMm vTS gene with a thermal gradient. PCR amplification of DNA from the retroperitoneal fibromatosis tumor from MmuYN91 was performed with CODEHOPs derived from two conserved TS motifs, RHFGA and DMGLB. A gradient of annealing temperatures ranging from 55 to 70 o C was used. The expected 284-bp fragment is indicated. Lane temperatures: 1, 70°C; 2, 68.9°C; 3, 67.1°C; 4, 64.3°C; 5, 60.5°C; 6, 57.9°C; 7, 56.1°C; 8, 55.0°C.

    Journal: Journal of Virology

    Article Title: Analysis of 4.3 Kilobases of Divergent Locus B of Macaque Retroperitoneal Fibromatosis-Associated Herpesvirus Reveals a Close Similarity in Gene Sequence and Genome Organization to Kaposi's Sarcoma-Associated Herpesvirus

    doi: 10.1128/JVI.77.9.5084-5097.2003

    Figure Lengend Snippet: CODEHOP PCR amplification of a region of the RFHVMm vTS gene with a thermal gradient. PCR amplification of DNA from the retroperitoneal fibromatosis tumor from MmuYN91 was performed with CODEHOPs derived from two conserved TS motifs, RHFGA and DMGLB. A gradient of annealing temperatures ranging from 55 to 70 o C was used. The expected 284-bp fragment is indicated. Lane temperatures: 1, 70°C; 2, 68.9°C; 3, 67.1°C; 4, 64.3°C; 5, 60.5°C; 6, 57.9°C; 7, 56.1°C; 8, 55.0°C.

    Article Snippet: PCR amplification was then performed with the DRIPA (5′-TTCACGACAGGATACCCTACG-3′) sense and QIRQB (5′-CAGCTCCTCTTGTCTGATTTG-3′) antisense primers derived from the 5′ end of the RFHVMm DNA polymerase sequence (Fig. ), and the resulting PCR product was isolated with a QIAEX gel extraction kit (Qiagen), cloned (Perfectly blunt cloning kit; Novagen), and sequenced.

    Techniques: Polymerase Chain Reaction, Amplification, Derivative Assay

    Slowly migrating DNAs are converted into ocDNA by Taq (A) or T4 (B) DNA polymerase treatment. The blots were hybridized with a C1-sense RNA probe. The positions of ocDNA, linear DNA (linDNA), scDNA, and cssDNA forms of viral DNA are indicated. Slowly migrating viral DNAs are indicated with an asterisk (∗). (A) TNAs were extracted from wt protoplasts at 72 h posttransfection with pTOM6 alone (the two lanes on the right) or together with pTOM100C4(−) or pTOM100NT and analyzed directly (−) or following incubation with Taq DNA polymerase for the time indicated below. (B) TNAs were extracted from wt or transgenic (102.22) protoplasts at 72 h posttransfection with pSP97 (TYLCSV-ES[1]) and analyzed following a 1-h incubation with (+) or without (−) T4 DNA polymerase. Lane C, TNAs from a TYLCSV-infected tomato plant digested with Bgl II to show migration of linear DNA.

    Journal: Journal of Virology

    Article Title: Transgenically Expressed T-Rep of Tomato Yellow Leaf Curl Sardinia Virus Acts as a trans-Dominant-Negative Mutant, Inhibiting Viral Transcription and Replication

    doi: 10.1128/JVI.75.22.10573-10581.2001

    Figure Lengend Snippet: Slowly migrating DNAs are converted into ocDNA by Taq (A) or T4 (B) DNA polymerase treatment. The blots were hybridized with a C1-sense RNA probe. The positions of ocDNA, linear DNA (linDNA), scDNA, and cssDNA forms of viral DNA are indicated. Slowly migrating viral DNAs are indicated with an asterisk (∗). (A) TNAs were extracted from wt protoplasts at 72 h posttransfection with pTOM6 alone (the two lanes on the right) or together with pTOM100C4(−) or pTOM100NT and analyzed directly (−) or following incubation with Taq DNA polymerase for the time indicated below. (B) TNAs were extracted from wt or transgenic (102.22) protoplasts at 72 h posttransfection with pSP97 (TYLCSV-ES[1]) and analyzed following a 1-h incubation with (+) or without (−) T4 DNA polymerase. Lane C, TNAs from a TYLCSV-infected tomato plant digested with Bgl II to show migration of linear DNA.

    Article Snippet: To verify the nature of these slowly migrating molecules, TNAs extracted from the samples at 24 and 72 h were incubated with Taq DNA polymerase and analyzed by Southern blot (Fig. B).

    Techniques: Incubation, Transgenic Assay, Infection, Migration

    Slowly migrating DNAs also accumulate at early stages after transfection of wt protoplasts with TYLCSV. TNAs were extracted from protoplasts transfected with pTOM6. The blots were hybridized with a C1-sense RNA probe. The positions of ocDNA, linear DNA (linDNA), scDNA, and cssDNA forms of viral DNA are indicated. Slow-migrating viral DNAs are indicated with an asterisk (∗). HPT, hours posttransfection. (A) Time course analysis of viral DNA forms. Sample at 72 h was diluted 20-fold to give signals of satisfactory intensity on the autoradiographic film. (B) Taq DNA polymerase treatment of TNAs at 24 and 72 h after transfection. Samples were incubated for 10 min at 37°C with (+) or without (−) the polymerase. Lane C, sample at 72 h digested with Bgl II to show the linear DNA.

    Journal: Journal of Virology

    Article Title: Transgenically Expressed T-Rep of Tomato Yellow Leaf Curl Sardinia Virus Acts as a trans-Dominant-Negative Mutant, Inhibiting Viral Transcription and Replication

    doi: 10.1128/JVI.75.22.10573-10581.2001

    Figure Lengend Snippet: Slowly migrating DNAs also accumulate at early stages after transfection of wt protoplasts with TYLCSV. TNAs were extracted from protoplasts transfected with pTOM6. The blots were hybridized with a C1-sense RNA probe. The positions of ocDNA, linear DNA (linDNA), scDNA, and cssDNA forms of viral DNA are indicated. Slow-migrating viral DNAs are indicated with an asterisk (∗). HPT, hours posttransfection. (A) Time course analysis of viral DNA forms. Sample at 72 h was diluted 20-fold to give signals of satisfactory intensity on the autoradiographic film. (B) Taq DNA polymerase treatment of TNAs at 24 and 72 h after transfection. Samples were incubated for 10 min at 37°C with (+) or without (−) the polymerase. Lane C, sample at 72 h digested with Bgl II to show the linear DNA.

    Article Snippet: To verify the nature of these slowly migrating molecules, TNAs extracted from the samples at 24 and 72 h were incubated with Taq DNA polymerase and analyzed by Southern blot (Fig. B).

    Techniques: Transfection, Incubation