Structured Review

PEQLAB dna polymerase
Dna Polymerase, supplied by PEQLAB, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dna polymerase/product/PEQLAB
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
dna polymerase - by Bioz Stars, 2020-04
86/100 stars

Images

Related Articles

Amplification:

Article Title: Production and characterization of amplified tumor-derived cRNA libraries to be used as vaccines against metastatic melanomas
Article Snippet: After termination of the reaction, 2 μl of cDNA were amplified using the Advantage 2 PCR kit (BD Biosciences Clontech). .. DNA polymerase was then inactivated with proteinase K and the cDNA library was digested with 200 U of Sfi I enzyme. cDNA libraries were then gel-purified on an 1% agarose gel and fragments from 300 bp to 10 kbp were extracted using E.Z.N.A.™ Gel Extraction kit from Peqlab GmbH (Erlangen, Germany).

Agarose Gel Electrophoresis:

Article Title: Production and characterization of amplified tumor-derived cRNA libraries to be used as vaccines against metastatic melanomas
Article Snippet: .. DNA polymerase was then inactivated with proteinase K and the cDNA library was digested with 200 U of Sfi I enzyme. cDNA libraries were then gel-purified on an 1% agarose gel and fragments from 300 bp to 10 kbp were extracted using E.Z.N.A.™ Gel Extraction kit from Peqlab GmbH (Erlangen, Germany). .. After precipitation, the cDNA library was ligated to dephosphorylated Sfi I-digested RNactive™ vector provided by CureVac GmbH (Tübingen, Germany) in three separated reactions to optimize vector/insert ratios.

Polymerase Chain Reaction:

Article Title: Production and characterization of amplified tumor-derived cRNA libraries to be used as vaccines against metastatic melanomas
Article Snippet: After termination of the reaction, 2 μl of cDNA were amplified using the Advantage 2 PCR kit (BD Biosciences Clontech). .. DNA polymerase was then inactivated with proteinase K and the cDNA library was digested with 200 U of Sfi I enzyme. cDNA libraries were then gel-purified on an 1% agarose gel and fragments from 300 bp to 10 kbp were extracted using E.Z.N.A.™ Gel Extraction kit from Peqlab GmbH (Erlangen, Germany).

Article Title: Mlc of Thermus thermophilus: a Glucose-Specific Regulator for a Glucose/Mannose ABC Transporter in the Absence of the Phosphotransferase System
Article Snippet: .. Proofreading DNA polymerase ( Pwo [Peqlab] or Phusion [Finnzymes]) was used for all PCR applications. ..

Generated:

Article Title: Production and characterization of amplified tumor-derived cRNA libraries to be used as vaccines against metastatic melanomas
Article Snippet: cDNA library generation cDNA libraries of tumor total RNA were generated using the slightly modified Creator™ SMART™ PCR cDNA library construction kit from BD Biosciences Clontech (Heidelberg, Germany). .. DNA polymerase was then inactivated with proteinase K and the cDNA library was digested with 200 U of Sfi I enzyme. cDNA libraries were then gel-purified on an 1% agarose gel and fragments from 300 bp to 10 kbp were extracted using E.Z.N.A.™ Gel Extraction kit from Peqlab GmbH (Erlangen, Germany).

cDNA Library Assay:

Article Title: Production and characterization of amplified tumor-derived cRNA libraries to be used as vaccines against metastatic melanomas
Article Snippet: .. DNA polymerase was then inactivated with proteinase K and the cDNA library was digested with 200 U of Sfi I enzyme. cDNA libraries were then gel-purified on an 1% agarose gel and fragments from 300 bp to 10 kbp were extracted using E.Z.N.A.™ Gel Extraction kit from Peqlab GmbH (Erlangen, Germany). .. After precipitation, the cDNA library was ligated to dephosphorylated Sfi I-digested RNactive™ vector provided by CureVac GmbH (Tübingen, Germany) in three separated reactions to optimize vector/insert ratios.

Modification:

Article Title: Production and characterization of amplified tumor-derived cRNA libraries to be used as vaccines against metastatic melanomas
Article Snippet: cDNA library generation cDNA libraries of tumor total RNA were generated using the slightly modified Creator™ SMART™ PCR cDNA library construction kit from BD Biosciences Clontech (Heidelberg, Germany). .. DNA polymerase was then inactivated with proteinase K and the cDNA library was digested with 200 U of Sfi I enzyme. cDNA libraries were then gel-purified on an 1% agarose gel and fragments from 300 bp to 10 kbp were extracted using E.Z.N.A.™ Gel Extraction kit from Peqlab GmbH (Erlangen, Germany).

Gel Extraction:

Article Title: Production and characterization of amplified tumor-derived cRNA libraries to be used as vaccines against metastatic melanomas
Article Snippet: .. DNA polymerase was then inactivated with proteinase K and the cDNA library was digested with 200 U of Sfi I enzyme. cDNA libraries were then gel-purified on an 1% agarose gel and fragments from 300 bp to 10 kbp were extracted using E.Z.N.A.™ Gel Extraction kit from Peqlab GmbH (Erlangen, Germany). .. After precipitation, the cDNA library was ligated to dephosphorylated Sfi I-digested RNactive™ vector provided by CureVac GmbH (Tübingen, Germany) in three separated reactions to optimize vector/insert ratios.

Plasmid Preparation:

Article Title: Production and characterization of amplified tumor-derived cRNA libraries to be used as vaccines against metastatic melanomas
Article Snippet: DNA polymerase was then inactivated with proteinase K and the cDNA library was digested with 200 U of Sfi I enzyme. cDNA libraries were then gel-purified on an 1% agarose gel and fragments from 300 bp to 10 kbp were extracted using E.Z.N.A.™ Gel Extraction kit from Peqlab GmbH (Erlangen, Germany). .. After precipitation, the cDNA library was ligated to dephosphorylated Sfi I-digested RNactive™ vector provided by CureVac GmbH (Tübingen, Germany) in three separated reactions to optimize vector/insert ratios.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    PEQLAB taq dna polymerase
    Enzymatic activity measurement of <t>Taq</t> . A : Schematic of the ss-dsDNA which is converted to dsDNA during dNMP incorporation. As a result, the polymerase moves outward along the <t>DNA</t> nanolever (which increases the friction and slows the DNA switching) and the dye is being held at a greater distance from the surface by the rigid double helix when the DNA is standing (which increases the fluorescence). B : Taq was bound to ss-dsDNA and baselines are recorded for 1.5 min (no dissociation). The injection of a dNTP-mix (100 μ M ) leads to a rapid incorporation of nucleotides ( DR up -drop and F up -rise), followed by the dissociation of polymerase from the dsDNA end (increasing DR up and slightly decreasing F up ). C : Temperature-dependent real-time elongation measurements ( c dNTP = 100 μ M ). Lines are single exponential fits ∝ exp {− t }. D : Elongation rates from C as a function of temperature, the line is an Arrhenius fit.
    Taq Dna Polymerase, supplied by PEQLAB, used in various techniques. Bioz Stars score: 94/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq dna polymerase/product/PEQLAB
    Average 94 stars, based on 33 article reviews
    Price from $9.99 to $1999.99
    taq dna polymerase - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    93
    PEQLAB kapa hifi dna polymerase
    Sequencing error rate distribution. a) Error rate distribution along the full-length fragment (421 bp) after analysis of 15,000 reads of L. pneumophila amplified with HotStarTaq ( open blue circles ) and <t>KAPA</t> <t>HiFi</t> <t>DNA</t> polymerases ( red open circles ). LOESS curves illustrate the reduced error rate with KAPA HiFi polymerase and the higher error rate in fragment covered by reverse reads. b) Comparison of error rate per base position with HotStarTaq and KAPA HiFi enzymes. A linear regression model explains the relationship between the two variables. Coefficient of determination (R 2 ) of 0.73
    Kapa Hifi Dna Polymerase, supplied by PEQLAB, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kapa hifi dna polymerase/product/PEQLAB
    Average 93 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    kapa hifi dna polymerase - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    92
    PEQLAB kapa dna polymerase
    Sequencing error rate distribution. a) Error rate distribution along the full-length fragment (421 bp) after analysis of 15,000 reads of L. pneumophila amplified with HotStarTaq ( open blue circles ) and <t>KAPA</t> <t>HiFi</t> <t>DNA</t> polymerases ( red open circles ). LOESS curves illustrate the reduced error rate with KAPA HiFi polymerase and the higher error rate in fragment covered by reverse reads. b) Comparison of error rate per base position with HotStarTaq and KAPA HiFi enzymes. A linear regression model explains the relationship between the two variables. Coefficient of determination (R 2 ) of 0.73
    Kapa Dna Polymerase, supplied by PEQLAB, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kapa dna polymerase/product/PEQLAB
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    kapa dna polymerase - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    86
    PEQLAB dna polymerase
    Sequencing error rate distribution. a) Error rate distribution along the full-length fragment (421 bp) after analysis of 15,000 reads of L. pneumophila amplified with HotStarTaq ( open blue circles ) and <t>KAPA</t> <t>HiFi</t> <t>DNA</t> polymerases ( red open circles ). LOESS curves illustrate the reduced error rate with KAPA HiFi polymerase and the higher error rate in fragment covered by reverse reads. b) Comparison of error rate per base position with HotStarTaq and KAPA HiFi enzymes. A linear regression model explains the relationship between the two variables. Coefficient of determination (R 2 ) of 0.73
    Dna Polymerase, supplied by PEQLAB, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna polymerase/product/PEQLAB
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna polymerase - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    Image Search Results


    Enzymatic activity measurement of Taq . A : Schematic of the ss-dsDNA which is converted to dsDNA during dNMP incorporation. As a result, the polymerase moves outward along the DNA nanolever (which increases the friction and slows the DNA switching) and the dye is being held at a greater distance from the surface by the rigid double helix when the DNA is standing (which increases the fluorescence). B : Taq was bound to ss-dsDNA and baselines are recorded for 1.5 min (no dissociation). The injection of a dNTP-mix (100 μ M ) leads to a rapid incorporation of nucleotides ( DR up -drop and F up -rise), followed by the dissociation of polymerase from the dsDNA end (increasing DR up and slightly decreasing F up ). C : Temperature-dependent real-time elongation measurements ( c dNTP = 100 μ M ). Lines are single exponential fits ∝ exp {− t }. D : Elongation rates from C as a function of temperature, the line is an Arrhenius fit.

    Journal: Scientific Reports

    Article Title: Polymerase/DNA interactions and enzymatic activity: multi-parameter analysis with electro-switchable biosurfaces

    doi: 10.1038/srep12066

    Figure Lengend Snippet: Enzymatic activity measurement of Taq . A : Schematic of the ss-dsDNA which is converted to dsDNA during dNMP incorporation. As a result, the polymerase moves outward along the DNA nanolever (which increases the friction and slows the DNA switching) and the dye is being held at a greater distance from the surface by the rigid double helix when the DNA is standing (which increases the fluorescence). B : Taq was bound to ss-dsDNA and baselines are recorded for 1.5 min (no dissociation). The injection of a dNTP-mix (100 μ M ) leads to a rapid incorporation of nucleotides ( DR up -drop and F up -rise), followed by the dissociation of polymerase from the dsDNA end (increasing DR up and slightly decreasing F up ). C : Temperature-dependent real-time elongation measurements ( c dNTP = 100 μ M ). Lines are single exponential fits ∝ exp {− t }. D : Elongation rates from C as a function of temperature, the line is an Arrhenius fit.

    Article Snippet: The Taq DNA polymerase and deoxyribonucleotides (dNTPs) were obtained from Peqlab (Erlangen, Germany).

    Techniques: Activity Assay, Fluorescence, Injection

    Sequencing error rate distribution. a) Error rate distribution along the full-length fragment (421 bp) after analysis of 15,000 reads of L. pneumophila amplified with HotStarTaq ( open blue circles ) and KAPA HiFi DNA polymerases ( red open circles ). LOESS curves illustrate the reduced error rate with KAPA HiFi polymerase and the higher error rate in fragment covered by reverse reads. b) Comparison of error rate per base position with HotStarTaq and KAPA HiFi enzymes. A linear regression model explains the relationship between the two variables. Coefficient of determination (R 2 ) of 0.73

    Journal: BMC Microbiology

    Article Title: Development of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems

    doi: 10.1186/s12866-017-0987-5

    Figure Lengend Snippet: Sequencing error rate distribution. a) Error rate distribution along the full-length fragment (421 bp) after analysis of 15,000 reads of L. pneumophila amplified with HotStarTaq ( open blue circles ) and KAPA HiFi DNA polymerases ( red open circles ). LOESS curves illustrate the reduced error rate with KAPA HiFi polymerase and the higher error rate in fragment covered by reverse reads. b) Comparison of error rate per base position with HotStarTaq and KAPA HiFi enzymes. A linear regression model explains the relationship between the two variables. Coefficient of determination (R 2 ) of 0.73

    Article Snippet: The Legionella -specific PCR reaction, using KAPA HiFi DNA polymerase, had the following components (50 μl total volume): KAPA HiFi HotStart ReadyMix 1X (Peqlab, Erlangen, Germany), 0.3 μM of each primer and 5 ng of the extracted environmental DNA.

    Techniques: Sequencing, Amplification