Structured Review

Kapa Biosystems dna polymerase
(a) UGT1A1 (TA) n promoter <t>PCR</t> products on 15.0% acrylamide gel electrophoresis stained with Ag-nitrate. Wells 1 and 4: 6/6 TA repeats (71 bp); well 2: 7/7 TA repeats (73 bp); well 5: 6/7 TA repeats (71 and 73 bp); well 3: 5 bp <t>DNA</t> ladder. (b) Electophoretograms obtained with fragment length analysis of UGT1A1 (TA) n promoter repeats. The upper peak is 7/7 TA repeats (102 bp); the lower peak is 6/6 TA repeats (100 bp). (c) Electophoretogram obtained with sequencing analysis of promoter of UGT1A1 gene with 6/6 TA repeats. (d) Electophoretogram obtained with sequencing analysis of promoter of UGT1A1 gene with 7/7 TA repeats.
Dna Polymerase, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 97/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "UGT1A1 (TA)n Promoter Genotype: Diagnostic and Population Pharmacogenetic Marker in Serbia"

Article Title: UGT1A1 (TA)n Promoter Genotype: Diagnostic and Population Pharmacogenetic Marker in Serbia

Journal: Balkan Journal of Medical Genetics : BJMG

doi: 10.2478/bjmg-2018-0012

(a) UGT1A1 (TA) n promoter PCR products on 15.0% acrylamide gel electrophoresis stained with Ag-nitrate. Wells 1 and 4: 6/6 TA repeats (71 bp); well 2: 7/7 TA repeats (73 bp); well 5: 6/7 TA repeats (71 and 73 bp); well 3: 5 bp DNA ladder. (b) Electophoretograms obtained with fragment length analysis of UGT1A1 (TA) n promoter repeats. The upper peak is 7/7 TA repeats (102 bp); the lower peak is 6/6 TA repeats (100 bp). (c) Electophoretogram obtained with sequencing analysis of promoter of UGT1A1 gene with 6/6 TA repeats. (d) Electophoretogram obtained with sequencing analysis of promoter of UGT1A1 gene with 7/7 TA repeats.
Figure Legend Snippet: (a) UGT1A1 (TA) n promoter PCR products on 15.0% acrylamide gel electrophoresis stained with Ag-nitrate. Wells 1 and 4: 6/6 TA repeats (71 bp); well 2: 7/7 TA repeats (73 bp); well 5: 6/7 TA repeats (71 and 73 bp); well 3: 5 bp DNA ladder. (b) Electophoretograms obtained with fragment length analysis of UGT1A1 (TA) n promoter repeats. The upper peak is 7/7 TA repeats (102 bp); the lower peak is 6/6 TA repeats (100 bp). (c) Electophoretogram obtained with sequencing analysis of promoter of UGT1A1 gene with 6/6 TA repeats. (d) Electophoretogram obtained with sequencing analysis of promoter of UGT1A1 gene with 7/7 TA repeats.

Techniques Used: Polymerase Chain Reaction, Acrylamide Gel Assay, Electrophoresis, Staining, Sequencing

2) Product Images from "UGT1A1 (TA)n Promoter Genotype: Diagnostic and Population Pharmacogenetic Marker in Serbia"

Article Title: UGT1A1 (TA)n Promoter Genotype: Diagnostic and Population Pharmacogenetic Marker in Serbia

Journal: Balkan Journal of Medical Genetics : BJMG

doi: 10.2478/bjmg-2018-0012

(a) UGT1A1 (TA) n promoter PCR products on 15.0% acrylamide gel electrophoresis stained with Ag-nitrate. Wells 1 and 4: 6/6 TA repeats (71 bp); well 2: 7/7 TA repeats (73 bp); well 5: 6/7 TA repeats (71 and 73 bp); well 3: 5 bp DNA ladder. (b) Electophoretograms obtained with fragment length analysis of UGT1A1 (TA) n promoter repeats. The upper peak is 7/7 TA repeats (102 bp); the lower peak is 6/6 TA repeats (100 bp). (c) Electophoretogram obtained with sequencing analysis of promoter of UGT1A1 gene with 6/6 TA repeats. (d) Electophoretogram obtained with sequencing analysis of promoter of UGT1A1 gene with 7/7 TA repeats.
Figure Legend Snippet: (a) UGT1A1 (TA) n promoter PCR products on 15.0% acrylamide gel electrophoresis stained with Ag-nitrate. Wells 1 and 4: 6/6 TA repeats (71 bp); well 2: 7/7 TA repeats (73 bp); well 5: 6/7 TA repeats (71 and 73 bp); well 3: 5 bp DNA ladder. (b) Electophoretograms obtained with fragment length analysis of UGT1A1 (TA) n promoter repeats. The upper peak is 7/7 TA repeats (102 bp); the lower peak is 6/6 TA repeats (100 bp). (c) Electophoretogram obtained with sequencing analysis of promoter of UGT1A1 gene with 6/6 TA repeats. (d) Electophoretogram obtained with sequencing analysis of promoter of UGT1A1 gene with 7/7 TA repeats.

Techniques Used: Polymerase Chain Reaction, Acrylamide Gel Assay, Electrophoresis, Staining, Sequencing

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Clone Assay:

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Amplification:

Article Title: Cloning and sequencing gB, gD, and gM genes to perform the genetic variability of bovine herpesvirus-1 from Indonesia
Article Snippet: Paragraph title: Alignment, amplification, and cloning the targeted gene ... Each round consisted of 2.5 mM MgCl2 , 0.3 mM for each of dNTPs, 1 U of DNA Polymerase, 10 pmol forward and reverse primer, and template DNA of < 100 ng per reaction until total volume was up to 50 µl for PCR grade water (KAPA HiFi HotStart ReadyMix, KAPABiosystem).

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Article Snippet: .. The amplification reaction was performed in a total volume of 30 μL, and the reaction mix contained 10 pmol of each primer, 50-100 ng of genomic DNA, 0.5 mM of each dNTP (Fermentas), 1 × PCR reaction buffer, 1.4 mM MgCl2 , 1 U DNA polymerase (KAPA Biosystems, Wilmington, MA, USA). .. The temperature profile of the PCR reactions was for the initial activation of DNA polymerase set at 95 °C for 15 min., followed by 35 cycles of 30 seconds denaturation at 95 °C, 30 seconds annealing as given in , and 30 seconds elongation at 72 °C, ending with a final extension period of 10 min. at 72 °C.

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Synthesized:

Article Title: Parallel identification of novel antimicrobial peptide sequences from multiple anuran species by targeted DNA sequencing
Article Snippet: First strand cDNA was synthesized from 1 μg of total RNA using the qScript™ Flex cDNA Synthesis Kit (Quanta Biosciences, Gaithersburg, Maryland, USA) according to the manufacturers’ instructions. .. The mixture for outer PCR contained 0.2 μl of DNA polymerase (5 U/μl), 2.5 μl of 10× buffer A (KAPA Taq PCR Kit, Kapa Biosystems, USA) together with 1 μl of 20× EvaGreen™ (Biotium, Fremont, California, USA), 0.5 μl of 10 μM dNTP solution, ~ 20 ng of nucleic acid from the primary PCR and 3 μl of 10 μM primer solution in a total volume of 20 μl.

SYBR Green Assay:

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Multiplex Assay:

Article Title: Plasmodium falciparum genetic crosses in a humanized mouse model
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Article Title: Direct repeat unit (dru) typing and antimicrobial resistance of methicillin-resistant Staphylococcus pseudintermedius isolated from dogs in Atlantic Canada
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Incubation:

Article Title: Direct repeat unit (dru) typing and antimicrobial resistance of methicillin-resistant Staphylococcus pseudintermedius isolated from dogs in Atlantic Canada
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Expressing:

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Transformation Assay:

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Derivative Assay:

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Concentration Assay:

Article Title: Targeted sequencing reveals distinct pathogenic variants in Chinese patients with lung adenocarcinoma brain metastases
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Generated:

Article Title: Plasmodium falciparum genetic crosses in a humanized mouse model
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Article Title: Targeted sequencing reveals distinct pathogenic variants in Chinese patients with lung adenocarcinoma brain metastases
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Article Title: Pooled Sequencing and Rare Variant Association Tests for Identifying the Determinants of Emerging Drug Resistance in Malaria Parasites
Article Snippet: Following ( ; ) we replaced the DNA polymerase with Kapa HiFi (Kapa Biosystems) and used Agencourt AMPure XP beads (Beckman Coulter) for sample purification. .. Raw Sequence data were demultiplexed and. fastq files generated using CASAVA 3.0 before further analysis.

Article Title: A New Broad Range Plasmid for DNA Delivery in Eukaryotic Cells Using Lactic Acid Bacteria: In Vitro and In Vivo Assays
Article Snippet: .. For this proposal, a 1,020 bp fragment was generated by PCR with a proofreading DNA polymerase (Kapa Biosystems) and the oligonucleotides PCMVFwd (5′ CCCGGG TTGACATTGATTATTGAC 3′) and PCMVRev (5′ GTCGAC CCATAGAGCCCACCGCAT 3′), introducing, respectively, a Sma I and Sal I (Invitrogen) (underlined) site in the fragment. .. The reaction mixture (final volume of 25 μL) contained: 100 ng template DNA; 1 unit of DNA polymerase; 5.0 μL Buffer Kapa CG 5X; deoxynucleoside triphosphates 25 mM, and primer forward and reverse 10 mM each.

other:

Article Title: A New Broad Range Plasmid for DNA Delivery in Eukaryotic Cells Using Lactic Acid Bacteria: In Vitro and In Vivo Assays
Article Snippet: The reaction mixture (final volume of 25 μL) contained: 100 ng template DNA; 1 unit of DNA polymerase; 5.0 μL Buffer Kapa CG 5X; deoxynucleoside triphosphates 25 mM, and primer forward and reverse 10 mM each.

Sequencing:

Article Title: Plasmodium falciparum genetic crosses in a humanized mouse model
Article Snippet: Paragraph title: Illumina sequencing and analysis of recombination parameters ... We replaced the DNA polymerase with Kapa HiFi (Kapa Biosystems) , and used Agencourt AMPure XP beads (Beckman Coulter) for sample purification.

Article Title: Targeted sequencing reveals distinct pathogenic variants in Chinese patients with lung adenocarcinoma brain metastases
Article Snippet: Paragraph title: Sequencing library construction ... The KAPA Library Quantification kit uses the novel KAPA SYBR FAST DNA Polymerase, engineered through a process of directed evolution for high-performance SYBR Green I-based qPCR (Kapa Biosystems, Inc., Wilmington, MA, USA).

Article Title: Cloning and sequencing gB, gD, and gM genes to perform the genetic variability of bovine herpesvirus-1 from Indonesia
Article Snippet: The alignment of gD showed 29 points of mutation of the sequence to differentiate between BHV-1 and BHV-5, and the alignment of gM showed 3 points mutation (at position of 507, 679, and 850), which could differentiate between BHV-1.1 and BHV-1.2. .. Each round consisted of 2.5 mM MgCl2 , 0.3 mM for each of dNTPs, 1 U of DNA Polymerase, 10 pmol forward and reverse primer, and template DNA of < 100 ng per reaction until total volume was up to 50 µl for PCR grade water (KAPA HiFi HotStart ReadyMix, KAPABiosystem).

Article Title: Pooled Sequencing and Rare Variant Association Tests for Identifying the Determinants of Emerging Drug Resistance in Malaria Parasites
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Article Title: UGT1A1 (TA)n Promoter Genotype: Diagnostic and Population Pharmacogenetic Marker in Serbia
Article Snippet: Ten percent of samples were randomly chosen and results of UGT1A1 (TA)n promoter genotyping by the PCR/acrylamide electrophoresis methodology were checked and confirmed using Sanger sequencing methodology. .. The amplification reaction was performed in a total volume of 30 μL, and the reaction mix contained 10 pmol of each primer, 50-100 ng of genomic DNA, 0.5 mM of each dNTP (Fermentas), 1 × PCR reaction buffer, 1.4 mM MgCl2 , 1 U DNA polymerase (KAPA Biosystems, Wilmington, MA, USA).

Article Title: A New Broad Range Plasmid for DNA Delivery in Eukaryotic Cells Using Lactic Acid Bacteria: In Vitro and In Vivo Assays
Article Snippet: For this proposal, a 1,020 bp fragment was generated by PCR with a proofreading DNA polymerase (Kapa Biosystems) and the oligonucleotides PCMVFwd (5′ CCCGGG TTGACATTGATTATTGAC 3′) and PCMVRev (5′ GTCGAC CCATAGAGCCCACCGCAT 3′), introducing, respectively, a Sma I and Sal I (Invitrogen) (underlined) site in the fragment. .. The nucleotide sequence of the insert was confirmed by sequencing using BigDye Terminator v3.1 no ABI 3500 (Thermo Scientific).

Article Title: Direct repeat unit (dru) typing and antimicrobial resistance of methicillin-resistant Staphylococcus pseudintermedius isolated from dogs in Atlantic Canada
Article Snippet: A 40-μL reaction was prepared containing 4 μL of template DNA, 0.8 U of DNA polymerase, buffer mixture containing 1.5 mM MgCl2 and 200 μM of each dNTP (KAPA2G Fast Hot Start DNA polymerase; KAPA Biosystems, Boston, Massachusetts, USA), additional 0.3 mM MgCl2 , and 0.8 μM of the forward primer dru GF (5′-GTTAGCATATTACCTCTCCTTGC-3′) and the reverse primer dru GR (5′-GCCGATTGTGCTTGATGAG-3′). .. The PCR products were purified using a kit (QIAquick PCR Purification Kit; Qiagen, Mississauga, Ontario) prior to sequencing.

Article Title: Parallel identification of novel antimicrobial peptide sequences from multiple anuran species by targeted DNA sequencing
Article Snippet: Paragraph title: Library construction and sequencing ... The mixture for outer PCR contained 0.2 μl of DNA polymerase (5 U/μl), 2.5 μl of 10× buffer A (KAPA Taq PCR Kit, Kapa Biosystems, USA) together with 1 μl of 20× EvaGreen™ (Biotium, Fremont, California, USA), 0.5 μl of 10 μM dNTP solution, ~ 20 ng of nucleic acid from the primary PCR and 3 μl of 10 μM primer solution in a total volume of 20 μl.

Binding Assay:

Article Title: Targeted sequencing reveals distinct pathogenic variants in Chinese patients with lung adenocarcinoma brain metastases
Article Snippet: The panel included 170 amplicons targeting fusion events for ALK, Ret proto-oncogene, proto-oncogene c-Ros1 and neurotrophic receptor tyrosine kinase, along with five genes (hydroxymethulbilane synthase, integrin subunit β 7, lamin A/C, MYC proto-oncogene, bHLH transcription factor and TATA-box binding protein) as internal expression controls. .. The KAPA Library Quantification kit uses the novel KAPA SYBR FAST DNA Polymerase, engineered through a process of directed evolution for high-performance SYBR Green I-based qPCR (Kapa Biosystems, Inc., Wilmington, MA, USA).

Multiplexing:

Article Title: Plasmodium falciparum genetic crosses in a humanized mouse model
Article Snippet: We replaced the DNA polymerase with Kapa HiFi (Kapa Biosystems) , and used Agencourt AMPure XP beads (Beckman Coulter) for sample purification. .. The Kapa SYBR Fast ABI Prism qPCR kit (Kapa Biosystems) was used to quantify templates before multiplexing (13 samples/lane) and sequenced on an Illumina HiSeq 2500.

In Vivo:

Article Title: Engineering processive DNA polymerases with maximum benefit at minimum cost
Article Snippet: Paragraph title: USING GENETIC APPROACHES TO IDENTIFY “BIOTECH” DNA POLYMERASES: IN VIVO AND IN VITRO SELECTION METHODS ... There are no reports to link amino acid substitutions in the Kofu DNA polymerase with specific polymerase properties and the amino acid substitutions in the DNA polymerase marketed as KAPA HiFi DNA polymerase by KAPA Biosystems Ltd., South Africa, are proprietary. suggest, however, that advantageous properties may require the combined action of several amino acid substitutions.

Mutagenesis:

Article Title: Engineering processive DNA polymerases with maximum benefit at minimum cost
Article Snippet: Several mutant DNA polymerases with increased processivity and other desirable features were identified, but many had multiple mutations as we observed for in vivo selections with the T4 DNA polymerase. .. There are no reports to link amino acid substitutions in the Kofu DNA polymerase with specific polymerase properties and the amino acid substitutions in the DNA polymerase marketed as KAPA HiFi DNA polymerase by KAPA Biosystems Ltd., South Africa, are proprietary. suggest, however, that advantageous properties may require the combined action of several amino acid substitutions.

Article Title: Cloning and sequencing gB, gD, and gM genes to perform the genetic variability of bovine herpesvirus-1 from Indonesia
Article Snippet: The alignment of gD showed 29 points of mutation of the sequence to differentiate between BHV-1 and BHV-5, and the alignment of gM showed 3 points mutation (at position of 507, 679, and 850), which could differentiate between BHV-1.1 and BHV-1.2. .. Each round consisted of 2.5 mM MgCl2 , 0.3 mM for each of dNTPs, 1 U of DNA Polymerase, 10 pmol forward and reverse primer, and template DNA of < 100 ng per reaction until total volume was up to 50 µl for PCR grade water (KAPA HiFi HotStart ReadyMix, KAPABiosystem).

Isolation:

Article Title: Direct repeat unit (dru) typing and antimicrobial resistance of methicillin-resistant Staphylococcus pseudintermedius isolated from dogs in Atlantic Canada
Article Snippet: Manufacturer’s guidelines were followed except a large loopful of bacterial colonies were suspended in 1.0 mL of polymerase chain reaction (PCR) water, and the incubation time at 56°C was increased from 30 min to 1 h. Isolated DNA was frozen at −20°C until use. .. A 40-μL reaction was prepared containing 4 μL of template DNA, 0.8 U of DNA polymerase, buffer mixture containing 1.5 mM MgCl2 and 200 μM of each dNTP (KAPA2G Fast Hot Start DNA polymerase; KAPA Biosystems, Boston, Massachusetts, USA), additional 0.3 mM MgCl2 , and 0.8 μM of the forward primer dru GF (5′-GTTAGCATATTACCTCTCCTTGC-3′) and the reverse primer dru GR (5′-GCCGATTGTGCTTGATGAG-3′).

Size-exclusion Chromatography:

Article Title: Targeted sequencing reveals distinct pathogenic variants in Chinese patients with lung adenocarcinoma brain metastases
Article Snippet: The KAPA Library Quantification kit uses the novel KAPA SYBR FAST DNA Polymerase, engineered through a process of directed evolution for high-performance SYBR Green I-based qPCR (Kapa Biosystems, Inc., Wilmington, MA, USA). .. The PCR cycling conditions were: initial denaturation: 95°C for 5 min; cycling: 35 cycles of 95°C for 30 sec and 60°C for 45 sec.

Purification:

Article Title: Plasmodium falciparum genetic crosses in a humanized mouse model
Article Snippet: .. We replaced the DNA polymerase with Kapa HiFi (Kapa Biosystems) , and used Agencourt AMPure XP beads (Beckman Coulter) for sample purification. .. The Kapa SYBR Fast ABI Prism qPCR kit (Kapa Biosystems) was used to quantify templates before multiplexing (13 samples/lane) and sequenced on an Illumina HiSeq 2500.

Article Title: Pooled Sequencing and Rare Variant Association Tests for Identifying the Determinants of Emerging Drug Resistance in Malaria Parasites
Article Snippet: .. Following ( ; ) we replaced the DNA polymerase with Kapa HiFi (Kapa Biosystems) and used Agencourt AMPure XP beads (Beckman Coulter) for sample purification. .. The Kapa SYBR Fast ABI Prism qPCR kit (Kapa Biosystems) was used to quantify templates before they were multiplexed (12 samples/lane) and sequenced on an Illumina HiSeq 2000.

Article Title: Direct repeat unit (dru) typing and antimicrobial resistance of methicillin-resistant Staphylococcus pseudintermedius isolated from dogs in Atlantic Canada
Article Snippet: A 40-μL reaction was prepared containing 4 μL of template DNA, 0.8 U of DNA polymerase, buffer mixture containing 1.5 mM MgCl2 and 200 μM of each dNTP (KAPA2G Fast Hot Start DNA polymerase; KAPA Biosystems, Boston, Massachusetts, USA), additional 0.3 mM MgCl2 , and 0.8 μM of the forward primer dru GF (5′-GTTAGCATATTACCTCTCCTTGC-3′) and the reverse primer dru GR (5′-GCCGATTGTGCTTGATGAG-3′). .. The PCR products were purified using a kit (QIAquick PCR Purification Kit; Qiagen, Mississauga, Ontario) prior to sequencing.

Polymerase Chain Reaction:

Article Title: Targeted sequencing reveals distinct pathogenic variants in Chinese patients with lung adenocarcinoma brain metastases
Article Snippet: The KAPA Library Quantification kit uses the novel KAPA SYBR FAST DNA Polymerase, engineered through a process of directed evolution for high-performance SYBR Green I-based qPCR (Kapa Biosystems, Inc., Wilmington, MA, USA). .. The PCR cycling conditions were: initial denaturation: 95°C for 5 min; cycling: 35 cycles of 95°C for 30 sec and 60°C for 45 sec.

Article Title: UGT1A1 (TA)n Promoter Genotype: Diagnostic and Population Pharmacogenetic Marker in Serbia
Article Snippet: .. The temperature profile of the PCR reactions was for the initial activation of DNA polymerase set at 95 °C for 15 min., followed by 35 cycles of 30 seconds denaturation at 95 °C, 30 seconds annealing as given in , and 30 seconds elongation at 72 °C, ending with a final extension period of 10 min. at 72 °C. .. The PCR fragments were visualized on 2.0% agarose gel, separated on an ABI PRISM® 3130 DNA analyzer (Applied Biosystems) and the collected data were analyzed with the Gene Mapper version 4 software (Applied Biosystems).

Article Title: Cloning and sequencing gB, gD, and gM genes to perform the genetic variability of bovine herpesvirus-1 from Indonesia
Article Snippet: .. Each round consisted of 2.5 mM MgCl2 , 0.3 mM for each of dNTPs, 1 U of DNA Polymerase, 10 pmol forward and reverse primer, and template DNA of < 100 ng per reaction until total volume was up to 50 µl for PCR grade water (KAPA HiFi HotStart ReadyMix, KAPABiosystem). .. The optimization of the PCR of gB was performed as follows: Initial denaturation for 3 min at 95°C, denaturation for 20 s at 98°C, annealing for 15 s at 53°C which were run in 35 cycles, while the extension lasted for 15 s at 72°C and the final extension lasted for 5 min at 72°C.

Article Title: UGT1A1 (TA)n Promoter Genotype: Diagnostic and Population Pharmacogenetic Marker in Serbia
Article Snippet: .. The amplification reaction was performed in a total volume of 30 μL, and the reaction mix contained 10 pmol of each primer, 50-100 ng of genomic DNA, 0.5 mM of each dNTP (Fermentas), 1 × PCR reaction buffer, 1.4 mM MgCl2 , 1 U DNA polymerase (KAPA Biosystems, Wilmington, MA, USA). .. The temperature profile of the PCR reactions was for the initial activation of DNA polymerase set at 95 °C for 15 min., followed by 35 cycles of 30 seconds denaturation at 95 °C, 30 seconds annealing as given in , and 30 seconds elongation at 72 °C, ending with a final extension period of 10 min. at 72 °C.

Article Title: Direct repeat unit (dru) typing and antimicrobial resistance of methicillin-resistant Staphylococcus pseudintermedius isolated from dogs in Atlantic Canada
Article Snippet: A multiplex PCR reaction was used to identify coagulase-positive staphylococci to the species level based on partial amplification of the nuc gene locus ( ). .. A 40-μL reaction was prepared containing 4 μL of template DNA, 0.8 U of DNA polymerase, buffer mixture containing 1.5 mM MgCl2 and 200 μM of each dNTP (KAPA2G Fast Hot Start DNA polymerase; KAPA Biosystems, Boston, Massachusetts, USA), additional 0.3 mM MgCl2 , and 0.8 μM of the forward primer dru GF (5′-GTTAGCATATTACCTCTCCTTGC-3′) and the reverse primer dru GR (5′-GCCGATTGTGCTTGATGAG-3′).

Plasmid Preparation:

Article Title: A New Broad Range Plasmid for DNA Delivery in Eukaryotic Cells Using Lactic Acid Bacteria: In Vitro and In Vivo Assays
Article Snippet: Paragraph title: Construction of a New Shuttle Vector, pExu ... For this proposal, a 1,020 bp fragment was generated by PCR with a proofreading DNA polymerase (Kapa Biosystems) and the oligonucleotides PCMVFwd (5′ CCCGGG TTGACATTGATTATTGAC 3′) and PCMVRev (5′ GTCGAC CCATAGAGCCCACCGCAT 3′), introducing, respectively, a Sma I and Sal I (Invitrogen) (underlined) site in the fragment.

Software:

Article Title: Targeted sequencing reveals distinct pathogenic variants in Chinese patients with lung adenocarcinoma brain metastases
Article Snippet: The KAPA Library Quantification kit uses the novel KAPA SYBR FAST DNA Polymerase, engineered through a process of directed evolution for high-performance SYBR Green I-based qPCR (Kapa Biosystems, Inc., Wilmington, MA, USA). .. The library concentration was calculated against the standard curve generated from 6 standards using qPCR software on the Bio-Rad CFX96 qPCR system (CFX Maestro software 12004110; Bio-Rad Laboratiories, Inc.).

Article Title: UGT1A1 (TA)n Promoter Genotype: Diagnostic and Population Pharmacogenetic Marker in Serbia
Article Snippet: The PCR products were separated on an ABI PRISM® 3130 DNA analyzer (Applied Biosystems, Foster City, CA, USA) and the collected data were analyzed with the GeneMapper version 4 software (Applied Biosystems). .. The amplification reaction was performed in a total volume of 30 μL, and the reaction mix contained 10 pmol of each primer, 50-100 ng of genomic DNA, 0.5 mM of each dNTP (Fermentas), 1 × PCR reaction buffer, 1.4 mM MgCl2 , 1 U DNA polymerase (KAPA Biosystems, Wilmington, MA, USA).

Real-time Polymerase Chain Reaction:

Article Title: Plasmodium falciparum genetic crosses in a humanized mouse model
Article Snippet: We replaced the DNA polymerase with Kapa HiFi (Kapa Biosystems) , and used Agencourt AMPure XP beads (Beckman Coulter) for sample purification. .. The Kapa SYBR Fast ABI Prism qPCR kit (Kapa Biosystems) was used to quantify templates before multiplexing (13 samples/lane) and sequenced on an Illumina HiSeq 2500.

Article Title: Targeted sequencing reveals distinct pathogenic variants in Chinese patients with lung adenocarcinoma brain metastases
Article Snippet: .. The KAPA Library Quantification kit uses the novel KAPA SYBR FAST DNA Polymerase, engineered through a process of directed evolution for high-performance SYBR Green I-based qPCR (Kapa Biosystems, Inc., Wilmington, MA, USA). .. The qPCR primers are 5′-AATGATACGGCGACCACCGA-3′ and 5′-CAAGCAGAAGACGGCATACGA-3′.

Article Title: Pooled Sequencing and Rare Variant Association Tests for Identifying the Determinants of Emerging Drug Resistance in Malaria Parasites
Article Snippet: Following ( ; ) we replaced the DNA polymerase with Kapa HiFi (Kapa Biosystems) and used Agencourt AMPure XP beads (Beckman Coulter) for sample purification. .. The Kapa SYBR Fast ABI Prism qPCR kit (Kapa Biosystems) was used to quantify templates before they were multiplexed (12 samples/lane) and sequenced on an Illumina HiSeq 2000.

Negative Control:

Article Title: Targeted sequencing reveals distinct pathogenic variants in Chinese patients with lung adenocarcinoma brain metastases
Article Snippet: No detectable DNA in the library prepared from non-template control (negative control) was used to indicate that no DNA contamination was introduced during library preparation. .. The KAPA Library Quantification kit uses the novel KAPA SYBR FAST DNA Polymerase, engineered through a process of directed evolution for high-performance SYBR Green I-based qPCR (Kapa Biosystems, Inc., Wilmington, MA, USA).

Selection:

Article Title: Engineering processive DNA polymerases with maximum benefit at minimum cost
Article Snippet: Paragraph title: USING GENETIC APPROACHES TO IDENTIFY “BIOTECH” DNA POLYMERASES: IN VIVO AND IN VITRO SELECTION METHODS ... There are no reports to link amino acid substitutions in the Kofu DNA polymerase with specific polymerase properties and the amino acid substitutions in the DNA polymerase marketed as KAPA HiFi DNA polymerase by KAPA Biosystems Ltd., South Africa, are proprietary. suggest, however, that advantageous properties may require the combined action of several amino acid substitutions.

Agarose Gel Electrophoresis:

Article Title: UGT1A1 (TA)n Promoter Genotype: Diagnostic and Population Pharmacogenetic Marker in Serbia
Article Snippet: The amplification reaction was performed in a total volume of 30 μL, and the reaction mix contained 10 pmol of each primer, 50-100 ng of genomic DNA, 0.5 mM of each dNTP (Fermentas), 1 × PCR reaction buffer, 1.4 mM MgCl2 , 1 U DNA polymerase (KAPA Biosystems, Wilmington, MA, USA). .. The PCR fragments were visualized on 2.0% agarose gel, separated on an ABI PRISM® 3130 DNA analyzer (Applied Biosystems) and the collected data were analyzed with the Gene Mapper version 4 software (Applied Biosystems).

Article Title: A New Broad Range Plasmid for DNA Delivery in Eukaryotic Cells Using Lactic Acid Bacteria: In Vitro and In Vivo Assays
Article Snippet: For this proposal, a 1,020 bp fragment was generated by PCR with a proofreading DNA polymerase (Kapa Biosystems) and the oligonucleotides PCMVFwd (5′ CCCGGG TTGACATTGATTATTGAC 3′) and PCMVRev (5′ GTCGAC CCATAGAGCCCACCGCAT 3′), introducing, respectively, a Sma I and Sal I (Invitrogen) (underlined) site in the fragment. .. The thermal cycling program used was as follows: initial denaturation at 95°C for 3 min, 20 cycles of 98°C for 20 s, 60°C for 15 s, and 72°C for 30 s. Finally, there was an extension step at 72°C for 2 min. After analyzing the correct size and purity on 1.0% agarose gel, the PCR product was cloned into Zero Blunt TOPO vector (Invitrogen) ( ) getting the Topo:CMV , this construction was established by transformation in E.coli Top10 ( ).

Article Title: Parallel identification of novel antimicrobial peptide sequences from multiple anuran species by targeted DNA sequencing
Article Snippet: The mixture for outer PCR contained 0.2 μl of DNA polymerase (5 U/μl), 2.5 μl of 10× buffer A (KAPA Taq PCR Kit, Kapa Biosystems, USA) together with 1 μl of 20× EvaGreen™ (Biotium, Fremont, California, USA), 0.5 μl of 10 μM dNTP solution, ~ 20 ng of nucleic acid from the primary PCR and 3 μl of 10 μM primer solution in a total volume of 20 μl. .. The quality of the amplification products was visualized by electrophoresis on 1.5% agarose gel after each amplification run.

In Vitro:

Article Title: Engineering processive DNA polymerases with maximum benefit at minimum cost
Article Snippet: Paragraph title: USING GENETIC APPROACHES TO IDENTIFY “BIOTECH” DNA POLYMERASES: IN VIVO AND IN VITRO SELECTION METHODS ... There are no reports to link amino acid substitutions in the Kofu DNA polymerase with specific polymerase properties and the amino acid substitutions in the DNA polymerase marketed as KAPA HiFi DNA polymerase by KAPA Biosystems Ltd., South Africa, are proprietary. suggest, however, that advantageous properties may require the combined action of several amino acid substitutions.

Electrophoresis:

Article Title: UGT1A1 (TA)n Promoter Genotype: Diagnostic and Population Pharmacogenetic Marker in Serbia
Article Snippet: Those samples were used as positive/negative controls on each acrylamide electrophoresis run [ ]. .. The amplification reaction was performed in a total volume of 30 μL, and the reaction mix contained 10 pmol of each primer, 50-100 ng of genomic DNA, 0.5 mM of each dNTP (Fermentas), 1 × PCR reaction buffer, 1.4 mM MgCl2 , 1 U DNA polymerase (KAPA Biosystems, Wilmington, MA, USA).

Article Title: Parallel identification of novel antimicrobial peptide sequences from multiple anuran species by targeted DNA sequencing
Article Snippet: The mixture for outer PCR contained 0.2 μl of DNA polymerase (5 U/μl), 2.5 μl of 10× buffer A (KAPA Taq PCR Kit, Kapa Biosystems, USA) together with 1 μl of 20× EvaGreen™ (Biotium, Fremont, California, USA), 0.5 μl of 10 μM dNTP solution, ~ 20 ng of nucleic acid from the primary PCR and 3 μl of 10 μM primer solution in a total volume of 20 μl. .. The quality of the amplification products was visualized by electrophoresis on 1.5% agarose gel after each amplification run.

Activation Assay:

Article Title: UGT1A1 (TA)n Promoter Genotype: Diagnostic and Population Pharmacogenetic Marker in Serbia
Article Snippet: .. The temperature profile of the PCR reactions was for the initial activation of DNA polymerase set at 95 °C for 15 min., followed by 35 cycles of 30 seconds denaturation at 95 °C, 30 seconds annealing as given in , and 30 seconds elongation at 72 °C, ending with a final extension period of 10 min. at 72 °C. .. The PCR fragments were visualized on 2.0% agarose gel, separated on an ABI PRISM® 3130 DNA analyzer (Applied Biosystems) and the collected data were analyzed with the Gene Mapper version 4 software (Applied Biosystems).

Article Title: UGT1A1 (TA)n Promoter Genotype: Diagnostic and Population Pharmacogenetic Marker in Serbia
Article Snippet: The amplification reaction was performed in a total volume of 30 μL, and the reaction mix contained 10 pmol of each primer, 50-100 ng of genomic DNA, 0.5 mM of each dNTP (Fermentas), 1 × PCR reaction buffer, 1.4 mM MgCl2 , 1 U DNA polymerase (KAPA Biosystems, Wilmington, MA, USA). .. The temperature profile of the PCR reactions was for the initial activation of DNA polymerase set at 95 °C for 15 min., followed by 35 cycles of 30 seconds denaturation at 95 °C, 30 seconds annealing as given in , and 30 seconds elongation at 72 °C, ending with a final extension period of 10 min. at 72 °C.

Recombinant:

Article Title: Plasmodium falciparum genetic crosses in a humanized mouse model
Article Snippet: Illumina sequencing and analysis of recombination parameters Two µg of DNA was sheared using a Covaris S-series sonicator (Covaris; duty cycle 20%, time 180 s, intensity 5, cycle burst 200, power 37 W, temperature 7 °C, mode freq sweeping) from the two parents (NF54HT-GFP–luc×NHP*) and 14 recombinant progeny from the cross. .. We replaced the DNA polymerase with Kapa HiFi (Kapa Biosystems) , and used Agencourt AMPure XP beads (Beckman Coulter) for sample purification.

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  • 97
    Kapa Biosystems dna polymerase
    (a) UGT1A1 (TA) n promoter <t>PCR</t> products on 15.0% acrylamide gel electrophoresis stained with Ag-nitrate. Wells 1 and 4: 6/6 TA repeats (71 bp); well 2: 7/7 TA repeats (73 bp); well 5: 6/7 TA repeats (71 and 73 bp); well 3: 5 bp <t>DNA</t> ladder. (b) Electophoretograms obtained with fragment length analysis of UGT1A1 (TA) n promoter repeats. The upper peak is 7/7 TA repeats (102 bp); the lower peak is 6/6 TA repeats (100 bp). (c) Electophoretogram obtained with sequencing analysis of promoter of UGT1A1 gene with 6/6 TA repeats. (d) Electophoretogram obtained with sequencing analysis of promoter of UGT1A1 gene with 7/7 TA repeats.
    Dna Polymerase, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 97/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna polymerase/product/Kapa Biosystems
    Average 97 stars, based on 5 article reviews
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    dna polymerase - by Bioz Stars, 2020-03
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    92
    Kapa Biosystems kapa 2gfast hotstart dna polymerase
    Electropherogram of singleplex PCR products using Kapa <t>2GFast</t> <t>HotStart</t> <t>DNA</t> polymerase. Singleplex PCR was performed with varied extension periods (15 s, 30 s, 60 s and 120 s) and with varied Mg 2+ concentrations (3.0 mM and 4.5 mM) using three pairs of locus-specific primers. The designed amplicon size is depicted below each lane.
    Kapa 2gfast Hotstart Dna Polymerase, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kapa 2gfast hotstart dna polymerase/product/Kapa Biosystems
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    kapa 2gfast hotstart dna polymerase - by Bioz Stars, 2020-03
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    86
    Kapa Biosystems pcr ready mix dna polymerase
    <t>RT-PCR</t> analysis of hsp70 mRNA expression level, (a). Lane 4,8,12, <t>DNA</t> molecular marker; lane 1, 2, 3, Group-1 (spleen, thymus, lymph node) resp; lane 5, 6, 7, Group-2 (spleen, thymus, lymph node) respectively; lane 9, 10, 11, Group-3 (spleen, thymus, lymph node) respectively; Lane 13, 14, 15, Group-4 (spleen, thymus, lymph node) resp. Group I- Control animals, Group II- Folate deficient diet fed animals, Group III- Control animals + aspartame, Group IV- Folate deficient diet fed animals + aspartame.
    Pcr Ready Mix Dna Polymerase, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    (a) UGT1A1 (TA) n promoter PCR products on 15.0% acrylamide gel electrophoresis stained with Ag-nitrate. Wells 1 and 4: 6/6 TA repeats (71 bp); well 2: 7/7 TA repeats (73 bp); well 5: 6/7 TA repeats (71 and 73 bp); well 3: 5 bp DNA ladder. (b) Electophoretograms obtained with fragment length analysis of UGT1A1 (TA) n promoter repeats. The upper peak is 7/7 TA repeats (102 bp); the lower peak is 6/6 TA repeats (100 bp). (c) Electophoretogram obtained with sequencing analysis of promoter of UGT1A1 gene with 6/6 TA repeats. (d) Electophoretogram obtained with sequencing analysis of promoter of UGT1A1 gene with 7/7 TA repeats.

    Journal: Balkan Journal of Medical Genetics : BJMG

    Article Title: UGT1A1 (TA)n Promoter Genotype: Diagnostic and Population Pharmacogenetic Marker in Serbia

    doi: 10.2478/bjmg-2018-0012

    Figure Lengend Snippet: (a) UGT1A1 (TA) n promoter PCR products on 15.0% acrylamide gel electrophoresis stained with Ag-nitrate. Wells 1 and 4: 6/6 TA repeats (71 bp); well 2: 7/7 TA repeats (73 bp); well 5: 6/7 TA repeats (71 and 73 bp); well 3: 5 bp DNA ladder. (b) Electophoretograms obtained with fragment length analysis of UGT1A1 (TA) n promoter repeats. The upper peak is 7/7 TA repeats (102 bp); the lower peak is 6/6 TA repeats (100 bp). (c) Electophoretogram obtained with sequencing analysis of promoter of UGT1A1 gene with 6/6 TA repeats. (d) Electophoretogram obtained with sequencing analysis of promoter of UGT1A1 gene with 7/7 TA repeats.

    Article Snippet: The amplification reaction was performed in a total volume of 30 μL, and the reaction mix contained 10 pmol of each primer, 50-100 ng of genomic DNA, 0.5 mM of each dNTP (Fermentas), 1 × PCR reaction buffer, 1.4 mM MgCl2 , 1 U DNA polymerase (KAPA Biosystems, Wilmington, MA, USA).

    Techniques: Polymerase Chain Reaction, Acrylamide Gel Assay, Electrophoresis, Staining, Sequencing

    Electropherogram of singleplex PCR products using Kapa 2GFast HotStart DNA polymerase. Singleplex PCR was performed with varied extension periods (15 s, 30 s, 60 s and 120 s) and with varied Mg 2+ concentrations (3.0 mM and 4.5 mM) using three pairs of locus-specific primers. The designed amplicon size is depicted below each lane.

    Journal: PLoS ONE

    Article Title: Highly Parallel and Short-Acting Amplification with Locus-Specific Primers to Detect Single Nucleotide Polymorphisms by the DigiTag2 Assay

    doi: 10.1371/journal.pone.0029967

    Figure Lengend Snippet: Electropherogram of singleplex PCR products using Kapa 2GFast HotStart DNA polymerase. Singleplex PCR was performed with varied extension periods (15 s, 30 s, 60 s and 120 s) and with varied Mg 2+ concentrations (3.0 mM and 4.5 mM) using three pairs of locus-specific primers. The designed amplicon size is depicted below each lane.

    Article Snippet: Labeling with Kapa 2GFast HotStart DNA polymerase The labeling reactions with Kapa 2GFast HotStart DNA polymerase had a final volume of 12 µl, including 6 µl of ligation products, 0.5 µM each labeled primer (Alexa555-cED-1 and Alexa647-cED-2), 2.5 nM each D1 primer (D1_i), 1.5× KAPA2G Buffer (including 2.25 mM Mg2+ ), an additional 2.25 mM Mg2+ (final concentration of Mg2+ : 4.5 mM), 0.2 mM dNTPs and 0.4 U of Kapa 2GFast HotStart DNA polymerase.

    Techniques: Polymerase Chain Reaction, Amplification

    RT-PCR analysis of hsp70 mRNA expression level, (a). Lane 4,8,12, DNA molecular marker; lane 1, 2, 3, Group-1 (spleen, thymus, lymph node) resp; lane 5, 6, 7, Group-2 (spleen, thymus, lymph node) respectively; lane 9, 10, 11, Group-3 (spleen, thymus, lymph node) respectively; Lane 13, 14, 15, Group-4 (spleen, thymus, lymph node) resp. Group I- Control animals, Group II- Folate deficient diet fed animals, Group III- Control animals + aspartame, Group IV- Folate deficient diet fed animals + aspartame.

    Journal: Journal of Biomedical Research

    Article Title: Effects of aspartame on hsp70, bcl-2 and bax expression in immune organs of Wistar albino rats

    doi: 10.7555/JBR.30.20140097

    Figure Lengend Snippet: RT-PCR analysis of hsp70 mRNA expression level, (a). Lane 4,8,12, DNA molecular marker; lane 1, 2, 3, Group-1 (spleen, thymus, lymph node) resp; lane 5, 6, 7, Group-2 (spleen, thymus, lymph node) respectively; lane 9, 10, 11, Group-3 (spleen, thymus, lymph node) respectively; Lane 13, 14, 15, Group-4 (spleen, thymus, lymph node) resp. Group I- Control animals, Group II- Folate deficient diet fed animals, Group III- Control animals + aspartame, Group IV- Folate deficient diet fed animals + aspartame.

    Article Snippet: Superscript-III reverse transcriptase was purchased from Invitrogen, (Carlsbad, CA, USA) and PCR Ready Mix DNA polymerase was purchased from KAPA Biosystem (USA).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Marker

    RT-PCR analysis of bcl-2 and bax mRNA expression level, (a). Lane 4,8,12, DNA molecular marker; lane 1, 2, 3, Group-1 (spleen, thymus, lymph node) resp; Lane 5, 6, 7, Group-2 (spleen, thymus, lymph node) resp; Lane 9, 10, 11 Group-3 (spleen, thymus, lymph node) resp; Lane 13, 14, 15 Group-4 (spleen, thymus, lymph node) resp. Group I- Control animals, Group 2- Folate deficient diet fed animals, Group 3- Control animals + aspartame, Group 4- Folate deficient diet fed animals + aspartame.

    Journal: Journal of Biomedical Research

    Article Title: Effects of aspartame on hsp70, bcl-2 and bax expression in immune organs of Wistar albino rats

    doi: 10.7555/JBR.30.20140097

    Figure Lengend Snippet: RT-PCR analysis of bcl-2 and bax mRNA expression level, (a). Lane 4,8,12, DNA molecular marker; lane 1, 2, 3, Group-1 (spleen, thymus, lymph node) resp; Lane 5, 6, 7, Group-2 (spleen, thymus, lymph node) resp; Lane 9, 10, 11 Group-3 (spleen, thymus, lymph node) resp; Lane 13, 14, 15 Group-4 (spleen, thymus, lymph node) resp. Group I- Control animals, Group 2- Folate deficient diet fed animals, Group 3- Control animals + aspartame, Group 4- Folate deficient diet fed animals + aspartame.

    Article Snippet: Superscript-III reverse transcriptase was purchased from Invitrogen, (Carlsbad, CA, USA) and PCR Ready Mix DNA polymerase was purchased from KAPA Biosystem (USA).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Marker