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Cosmo Genetech Co dna polymerase
Establishment of recipient founder cells. a Phase contrast ( left ) and fluorescent ( right ) microscopic images of CHO/P1[DsRed] cells. b Schematic drawing of transgene structure in the genome of CHO/P1[DsRed]. Locations of primers (F and R) for genomic <t>PCR</t> are indicated as arrows . c Genomic PCR analysis. Genomic integration of the P1/DsRed sequence was confirmed by genomic PCR using primers F and R. Lane M , Hin cII-digested φX174 and Hin dIII-digested λDNA molecular weight marker; 1 H 2 O, 2 parental CHO-K1, 3 CHO/P1[DsRed]. d Southern blot analysis. Genomic <t>DNA</t> digested with Bgl II was subjected to Southern blotting using a DsRed probe
Dna Polymerase, supplied by Cosmo Genetech Co, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 4 article reviews
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1) Product Images from "Repeated integration of antibody genes into a pre-selected chromosomal locus of CHO cells using an accumulative site-specific gene integration system"

Article Title: Repeated integration of antibody genes into a pre-selected chromosomal locus of CHO cells using an accumulative site-specific gene integration system

Journal: Cytotechnology

doi: 10.1007/s10616-011-9397-y

Establishment of recipient founder cells. a Phase contrast ( left ) and fluorescent ( right ) microscopic images of CHO/P1[DsRed] cells. b Schematic drawing of transgene structure in the genome of CHO/P1[DsRed]. Locations of primers (F and R) for genomic PCR are indicated as arrows . c Genomic PCR analysis. Genomic integration of the P1/DsRed sequence was confirmed by genomic PCR using primers F and R. Lane M , Hin cII-digested φX174 and Hin dIII-digested λDNA molecular weight marker; 1 H 2 O, 2 parental CHO-K1, 3 CHO/P1[DsRed]. d Southern blot analysis. Genomic DNA digested with Bgl II was subjected to Southern blotting using a DsRed probe
Figure Legend Snippet: Establishment of recipient founder cells. a Phase contrast ( left ) and fluorescent ( right ) microscopic images of CHO/P1[DsRed] cells. b Schematic drawing of transgene structure in the genome of CHO/P1[DsRed]. Locations of primers (F and R) for genomic PCR are indicated as arrows . c Genomic PCR analysis. Genomic integration of the P1/DsRed sequence was confirmed by genomic PCR using primers F and R. Lane M , Hin cII-digested φX174 and Hin dIII-digested λDNA molecular weight marker; 1 H 2 O, 2 parental CHO-K1, 3 CHO/P1[DsRed]. d Southern blot analysis. Genomic DNA digested with Bgl II was subjected to Southern blotting using a DsRed probe

Techniques Used: Polymerase Chain Reaction, Sequencing, Molecular Weight, Marker, Southern Blot

Generation of scFv-Fc producer cells using an accumulative gene integration system. a – c Schematic drawing of transgene structures in the genome of scFv-Fc producer cells ( a CHO/scFv-Fc x1, b CHO/scFv-Fc x2, c CHO/scFv-Fc x3). Location of primers (F and B, and A and R for CHO/scFv-Fc x1; F and N for CHO/scFv-Fc x2; F and B for CHO/scFv-Fc x3) are indicated by arrows . d – f Genomic PCR analysis after the first ( d ), second ( e ) and third ( f ) RMCE reactions. After each RMCE reaction, clones were established in selective medium containing the relevant antibiotics for screening. Lane M , Hin cII-digested φX174 and Hin dIII-digested λDNA molecular weight markers; 1 H 2 O, 2 CHO/P1[DsRed] ( d ), CHO/scFv-Fc x1 ( e ) or CHO/scFv-Fc x2 ( f ), 3 without Cre vector, 4 established clones, CHO/scFv-Fc x1 ( d ), CHO/scFv-Fc x2 ( e ) or CHO/scFv-Fc x3 ( f ). g Southern blot analysis. Genomic DNA samples digested with Eco RI and Apa I were subjected to Southern blotting using a scFv-Fc probe. Lane 1 CHO/P1[DsRed], 2 CHO/scFv-Fc x1, 3 CHO/scFv-Fc x2, 4 CHO/scFv-Fc x3
Figure Legend Snippet: Generation of scFv-Fc producer cells using an accumulative gene integration system. a – c Schematic drawing of transgene structures in the genome of scFv-Fc producer cells ( a CHO/scFv-Fc x1, b CHO/scFv-Fc x2, c CHO/scFv-Fc x3). Location of primers (F and B, and A and R for CHO/scFv-Fc x1; F and N for CHO/scFv-Fc x2; F and B for CHO/scFv-Fc x3) are indicated by arrows . d – f Genomic PCR analysis after the first ( d ), second ( e ) and third ( f ) RMCE reactions. After each RMCE reaction, clones were established in selective medium containing the relevant antibiotics for screening. Lane M , Hin cII-digested φX174 and Hin dIII-digested λDNA molecular weight markers; 1 H 2 O, 2 CHO/P1[DsRed] ( d ), CHO/scFv-Fc x1 ( e ) or CHO/scFv-Fc x2 ( f ), 3 without Cre vector, 4 established clones, CHO/scFv-Fc x1 ( d ), CHO/scFv-Fc x2 ( e ) or CHO/scFv-Fc x3 ( f ). g Southern blot analysis. Genomic DNA samples digested with Eco RI and Apa I were subjected to Southern blotting using a scFv-Fc probe. Lane 1 CHO/P1[DsRed], 2 CHO/scFv-Fc x1, 3 CHO/scFv-Fc x2, 4 CHO/scFv-Fc x3

Techniques Used: Polymerase Chain Reaction, Clone Assay, Molecular Weight, Plasmid Preparation, Southern Blot

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Polymerase Chain Reaction:

Article Title: Magnetic heating of nanoparticles as a scalable cryopreservation technology for human induced pluripotent stem cells
Article Snippet: .. PCR was initiated using a DNA polymerase (G-Taq; Cosmo Genetech, Seoul, Korea) at 94 °C for 2 min, followed by 35 cycles of amplification at 98 °C for 10 s, 57 °C for 30 s, and 68 °C for 30 s, and final extension at 30 °C for 5 min. ..

Article Title: Repeated integration of antibody genes into a pre-selected chromosomal locus of CHO cells using an accumulative site-specific gene integration system
Article Snippet: .. The PCR was initiated with DNA polymerase (G-Taq; Cosmo Genetech, Seoul, Korea) at 95 °C for 2 min, followed by 35 cycles of amplification at 95 °C for 30 s, 55–57 °C for 40 s, 72 °C for 15–70 s and 72 °C for 5 min for final extension. .. For Southern blot analysis, genomic DNA (10 μg) digested with suitable restriction enzymes was electrophoresed on a 0.8% (w/v) agarose gel and then transferred to a nylon membrane (Hybond-XL; GE Healthcare, Buckinghamshire, UK).

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Amplification:

Article Title: Magnetic heating of nanoparticles as a scalable cryopreservation technology for human induced pluripotent stem cells
Article Snippet: .. PCR was initiated using a DNA polymerase (G-Taq; Cosmo Genetech, Seoul, Korea) at 94 °C for 2 min, followed by 35 cycles of amplification at 98 °C for 10 s, 57 °C for 30 s, and 68 °C for 30 s, and final extension at 30 °C for 5 min. ..

Article Title: Repeated integration of antibody genes into a pre-selected chromosomal locus of CHO cells using an accumulative site-specific gene integration system
Article Snippet: .. The PCR was initiated with DNA polymerase (G-Taq; Cosmo Genetech, Seoul, Korea) at 95 °C for 2 min, followed by 35 cycles of amplification at 95 °C for 30 s, 55–57 °C for 40 s, 72 °C for 15–70 s and 72 °C for 5 min for final extension. .. For Southern blot analysis, genomic DNA (10 μg) digested with suitable restriction enzymes was electrophoresed on a 0.8% (w/v) agarose gel and then transferred to a nylon membrane (Hybond-XL; GE Healthcare, Buckinghamshire, UK).

Article Title: Oral Immunotherapy for Pollen Allergy Using T-Cell Epitope-Containing Egg White Derived from Genetically Manipulated Chickens
Article Snippet: .. PCR was initiated with DNA polymerase (G-Taq; Cosmo Genetech, Seoul, Korea) at 95°C for 2 min, followed by 35 cycles of amplification at 95°C for 20 s, 56°C for 40 s, 72°C for 10 s, and 72°C for 5 min for final extension. .. Therapeutic protocol for oral administration of egg white containing cLys-7crp to treat Cj pollinosis For therapeutic trials, we first generated an allergic rhinitis mouse model specific for Cj pollinosis.

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    Cosmo Genetech Co dna polymerase
    Establishment of recipient founder cells. a Phase contrast ( left ) and fluorescent ( right ) microscopic images of CHO/P1[DsRed] cells. b Schematic drawing of transgene structure in the genome of CHO/P1[DsRed]. Locations of primers (F and R) for genomic <t>PCR</t> are indicated as arrows . c Genomic PCR analysis. Genomic integration of the P1/DsRed sequence was confirmed by genomic PCR using primers F and R. Lane M , Hin cII-digested φX174 and Hin dIII-digested λDNA molecular weight marker; 1 H 2 O, 2 parental CHO-K1, 3 CHO/P1[DsRed]. d Southern blot analysis. Genomic <t>DNA</t> digested with Bgl II was subjected to Southern blotting using a DsRed probe
    Dna Polymerase, supplied by Cosmo Genetech Co, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna polymerase/product/Cosmo Genetech Co
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    dna polymerase - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    93
    Cosmo Genetech Co taq dna polymerase
    Establishment of recipient founder cells. a Phase contrast ( left ) and fluorescent ( right ) microscopic images of CHO/P1[DsRed] cells. b Schematic drawing of transgene structure in the genome of CHO/P1[DsRed]. Locations of primers (F and R) for genomic <t>PCR</t> are indicated as arrows . c Genomic PCR analysis. Genomic integration of the P1/DsRed sequence was confirmed by genomic PCR using primers F and R. Lane M , Hin cII-digested φX174 and Hin dIII-digested λDNA molecular weight marker; 1 H 2 O, 2 parental CHO-K1, 3 CHO/P1[DsRed]. d Southern blot analysis. Genomic <t>DNA</t> digested with Bgl II was subjected to Southern blotting using a DsRed probe
    Taq Dna Polymerase, supplied by Cosmo Genetech Co, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq dna polymerase/product/Cosmo Genetech Co
    Average 93 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    taq dna polymerase - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

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    Establishment of recipient founder cells. a Phase contrast ( left ) and fluorescent ( right ) microscopic images of CHO/P1[DsRed] cells. b Schematic drawing of transgene structure in the genome of CHO/P1[DsRed]. Locations of primers (F and R) for genomic PCR are indicated as arrows . c Genomic PCR analysis. Genomic integration of the P1/DsRed sequence was confirmed by genomic PCR using primers F and R. Lane M , Hin cII-digested φX174 and Hin dIII-digested λDNA molecular weight marker; 1 H 2 O, 2 parental CHO-K1, 3 CHO/P1[DsRed]. d Southern blot analysis. Genomic DNA digested with Bgl II was subjected to Southern blotting using a DsRed probe

    Journal: Cytotechnology

    Article Title: Repeated integration of antibody genes into a pre-selected chromosomal locus of CHO cells using an accumulative site-specific gene integration system

    doi: 10.1007/s10616-011-9397-y

    Figure Lengend Snippet: Establishment of recipient founder cells. a Phase contrast ( left ) and fluorescent ( right ) microscopic images of CHO/P1[DsRed] cells. b Schematic drawing of transgene structure in the genome of CHO/P1[DsRed]. Locations of primers (F and R) for genomic PCR are indicated as arrows . c Genomic PCR analysis. Genomic integration of the P1/DsRed sequence was confirmed by genomic PCR using primers F and R. Lane M , Hin cII-digested φX174 and Hin dIII-digested λDNA molecular weight marker; 1 H 2 O, 2 parental CHO-K1, 3 CHO/P1[DsRed]. d Southern blot analysis. Genomic DNA digested with Bgl II was subjected to Southern blotting using a DsRed probe

    Article Snippet: The PCR was initiated with DNA polymerase (G-Taq; Cosmo Genetech, Seoul, Korea) at 95 °C for 2 min, followed by 35 cycles of amplification at 95 °C for 30 s, 55–57 °C for 40 s, 72 °C for 15–70 s and 72 °C for 5 min for final extension.

    Techniques: Polymerase Chain Reaction, Sequencing, Molecular Weight, Marker, Southern Blot

    Generation of scFv-Fc producer cells using an accumulative gene integration system. a – c Schematic drawing of transgene structures in the genome of scFv-Fc producer cells ( a CHO/scFv-Fc x1, b CHO/scFv-Fc x2, c CHO/scFv-Fc x3). Location of primers (F and B, and A and R for CHO/scFv-Fc x1; F and N for CHO/scFv-Fc x2; F and B for CHO/scFv-Fc x3) are indicated by arrows . d – f Genomic PCR analysis after the first ( d ), second ( e ) and third ( f ) RMCE reactions. After each RMCE reaction, clones were established in selective medium containing the relevant antibiotics for screening. Lane M , Hin cII-digested φX174 and Hin dIII-digested λDNA molecular weight markers; 1 H 2 O, 2 CHO/P1[DsRed] ( d ), CHO/scFv-Fc x1 ( e ) or CHO/scFv-Fc x2 ( f ), 3 without Cre vector, 4 established clones, CHO/scFv-Fc x1 ( d ), CHO/scFv-Fc x2 ( e ) or CHO/scFv-Fc x3 ( f ). g Southern blot analysis. Genomic DNA samples digested with Eco RI and Apa I were subjected to Southern blotting using a scFv-Fc probe. Lane 1 CHO/P1[DsRed], 2 CHO/scFv-Fc x1, 3 CHO/scFv-Fc x2, 4 CHO/scFv-Fc x3

    Journal: Cytotechnology

    Article Title: Repeated integration of antibody genes into a pre-selected chromosomal locus of CHO cells using an accumulative site-specific gene integration system

    doi: 10.1007/s10616-011-9397-y

    Figure Lengend Snippet: Generation of scFv-Fc producer cells using an accumulative gene integration system. a – c Schematic drawing of transgene structures in the genome of scFv-Fc producer cells ( a CHO/scFv-Fc x1, b CHO/scFv-Fc x2, c CHO/scFv-Fc x3). Location of primers (F and B, and A and R for CHO/scFv-Fc x1; F and N for CHO/scFv-Fc x2; F and B for CHO/scFv-Fc x3) are indicated by arrows . d – f Genomic PCR analysis after the first ( d ), second ( e ) and third ( f ) RMCE reactions. After each RMCE reaction, clones were established in selective medium containing the relevant antibiotics for screening. Lane M , Hin cII-digested φX174 and Hin dIII-digested λDNA molecular weight markers; 1 H 2 O, 2 CHO/P1[DsRed] ( d ), CHO/scFv-Fc x1 ( e ) or CHO/scFv-Fc x2 ( f ), 3 without Cre vector, 4 established clones, CHO/scFv-Fc x1 ( d ), CHO/scFv-Fc x2 ( e ) or CHO/scFv-Fc x3 ( f ). g Southern blot analysis. Genomic DNA samples digested with Eco RI and Apa I were subjected to Southern blotting using a scFv-Fc probe. Lane 1 CHO/P1[DsRed], 2 CHO/scFv-Fc x1, 3 CHO/scFv-Fc x2, 4 CHO/scFv-Fc x3

    Article Snippet: The PCR was initiated with DNA polymerase (G-Taq; Cosmo Genetech, Seoul, Korea) at 95 °C for 2 min, followed by 35 cycles of amplification at 95 °C for 30 s, 55–57 °C for 40 s, 72 °C for 15–70 s and 72 °C for 5 min for final extension.

    Techniques: Polymerase Chain Reaction, Clone Assay, Molecular Weight, Plasmid Preparation, Southern Blot