Structured Review

Toyobo dna polymerase kod fx neo
Dna Polymerase Kod Fx Neo, supplied by Toyobo, used in various techniques. Bioz Stars score: 89/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dna polymerase kod fx neo/product/Toyobo
Average 89 stars, based on 12 article reviews
Price from $9.99 to $1999.99
dna polymerase kod fx neo - by Bioz Stars, 2020-09
89/100 stars

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Sequencing:

Article Title: Functional screening system for yeast-secreted peptides acting on G-protein coupled receptors
Article Snippet: .. The DNA fragments encoding GLP1, S2 -GLP1 and Ex4, with or without a FLAG-encoding sequence at the 3′ terminus, were double-stranded from oligonucleotides with the mutual complementary region using DNA polymerase KOD-FX-Neo (Toyobo). .. The double-stranded DNAs encoding those GLP1 analogues were introduced into the multiple cloning sites of pULS by In-Fusion (Clontech) and named pULS-GLP1, -GLP1FLAG, -S2 -GLP1, -S2 -GLP1FLAG, -Ex4 and -Ex4FLAG, respectively.

Article Title: Genomic analyses reveal low mitochondrial and high nuclear diversity in the cyclosporin-producing fungus Tolypocladium inflatum.
Article Snippet: .. Mitochondrial DNA is generally regarded to evolve faster than nuclear DNA in animals, whereas if this is also true in fungi remains unclear. ..

Polymerase Chain Reaction:

Article Title: A Collection of Transgenic Medaka Strains for Efficient Site-Directed Transgenesis Mediated by phiC31 Integrase
Article Snippet: .. The ligated DNA was used as the PCR template and amplified with the primers 3Ds-inverse-FW1 and 3Ds-inverse-RV1 (Table S1) and the DNA polymerase KOD FX Neo (Toyobo). .. The amplification reactions were performed as follows: 94° for 2 min, 35 cycles at 98° for 10 s each, 55° for 20 s, and 68° for 5 min.

Article Title: Presence of a Shared 5′-Leader Sequence in Ancestral Human and Mammalian Retroviruses and Its Transduction into Feline Leukemia Virus
Article Snippet: .. The proviruses were amplified from the chromosomal DNA of a female cat (Kyoto-SPF) by performing PCR with specific primers and the DNA polymerase KOD Fx Neo (Toyobo, Osaka, Japan). .. They were then cloned into pCR-Blunt II-TOPO vectors (Invitrogen, Carlsbad, CA, USA).

Article Title: Transposition of IS4 Family Insertion Sequences ISTeha3, ISTeha4, and ISTeha5 into the arc Operon Disrupts Arginine Deiminase System in Tetragenococcus halophilus
Article Snippet: .. PCR was performed using DNA polymerase KOD FX Neo (Toyobo, Osaka, Japan) under the optimal conditions recommended by the supplier. .. PCR products were separated on 0.9% agarose gel and purified using the Wizard SV gel and PCR clean-up system (Promega, Madison, WI).

Amplification:

Article Title: A Collection of Transgenic Medaka Strains for Efficient Site-Directed Transgenesis Mediated by phiC31 Integrase
Article Snippet: .. The ligated DNA was used as the PCR template and amplified with the primers 3Ds-inverse-FW1 and 3Ds-inverse-RV1 (Table S1) and the DNA polymerase KOD FX Neo (Toyobo). .. The amplification reactions were performed as follows: 94° for 2 min, 35 cycles at 98° for 10 s each, 55° for 20 s, and 68° for 5 min.

Article Title: Presence of a Shared 5′-Leader Sequence in Ancestral Human and Mammalian Retroviruses and Its Transduction into Feline Leukemia Virus
Article Snippet: .. The proviruses were amplified from the chromosomal DNA of a female cat (Kyoto-SPF) by performing PCR with specific primers and the DNA polymerase KOD Fx Neo (Toyobo, Osaka, Japan). .. They were then cloned into pCR-Blunt II-TOPO vectors (Invitrogen, Carlsbad, CA, USA).

Article Title: The genetic organization of the capsular polysaccharide biosynthesis region of Actinobacillus pleuropneumoniae serotype 14
Article Snippet: .. A touchdown PCR with DNA polymerase KOD-FX Neo (Toyobo, Osaka, Japan) was done for amplification of the cps region as recommended by the supplier. ..

Touchdown PCR:

Article Title: The genetic organization of the capsular polysaccharide biosynthesis region of Actinobacillus pleuropneumoniae serotype 14
Article Snippet: .. A touchdown PCR with DNA polymerase KOD-FX Neo (Toyobo, Osaka, Japan) was done for amplification of the cps region as recommended by the supplier. ..

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  • 91
    Toyobo fidelity dna polymerases
    <t>PCR</t> product patterns amplified by the primer pair CS1-F/CS1-R targeting the partial S gene. Samples from left to right: (1) JAi-23, (2) JMi-69, (3) JMi-124, (4) JKa-230, (5) JA0-56, (6) JMi-231, (M) <t>DNA</t> marker. The dashed arrows indicate products of PEDV variants with large genomic deletions (approximately 0.2–0.3 kbp) and the solid arrow indicates the predicted product (872 bp).
    Fidelity Dna Polymerases, supplied by Toyobo, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fidelity dna polymerases/product/Toyobo
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fidelity dna polymerases - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    91
    Toyobo kod fx neo dna polymerase
    <t>PCR</t> product patterns amplified by the primer pair CS1-F/CS1-R targeting the partial S gene. Samples from left to right: (1) JAi-23, (2) JMi-69, (3) JMi-124, (4) JKa-230, (5) JA0-56, (6) JMi-231, (M) <t>DNA</t> marker. The dashed arrows indicate products of PEDV variants with large genomic deletions (approximately 0.2–0.3 kbp) and the solid arrow indicates the predicted product (872 bp).
    Kod Fx Neo Dna Polymerase, supplied by Toyobo, used in various techniques. Bioz Stars score: 91/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kod fx neo dna polymerase/product/Toyobo
    Average 91 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    kod fx neo dna polymerase - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    Image Search Results


    PCR product patterns amplified by the primer pair CS1-F/CS1-R targeting the partial S gene. Samples from left to right: (1) JAi-23, (2) JMi-69, (3) JMi-124, (4) JKa-230, (5) JA0-56, (6) JMi-231, (M) DNA marker. The dashed arrows indicate products of PEDV variants with large genomic deletions (approximately 0.2–0.3 kbp) and the solid arrow indicates the predicted product (872 bp).

    Journal: PLoS ONE

    Article Title: Novel Porcine Epidemic Diarrhea Virus (PEDV) Variants with Large Deletions in the Spike (S) Gene Coexist with PEDV Strains Possessing an Intact S Gene in Domestic Pigs in Japan: A New Disease Situation

    doi: 10.1371/journal.pone.0170126

    Figure Lengend Snippet: PCR product patterns amplified by the primer pair CS1-F/CS1-R targeting the partial S gene. Samples from left to right: (1) JAi-23, (2) JMi-69, (3) JMi-124, (4) JKa-230, (5) JA0-56, (6) JMi-231, (M) DNA marker. The dashed arrows indicate products of PEDV variants with large genomic deletions (approximately 0.2–0.3 kbp) and the solid arrow indicates the predicted product (872 bp).

    Article Snippet: The same result was observed when one-step RT-PCR tests (AccessQuick RT-PCR System kit, Promega Corp, WI, USA) were performed from extracted RNA and PCR tests using other kits containing high fidelity DNA polymerases (KOD FX, KOD FX neo, and Blend–Tag Plus Kits, Toyobo Co., Japan) were performed from cDNA templates to amplify the CS1 fragment.

    Techniques: Polymerase Chain Reaction, Amplification, Marker