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Roche dna polymerase klenow
Dna Polymerase Klenow, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dna polymerase klenow/product/Roche
Average 91 stars, based on 1 article reviews
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dna polymerase klenow - by Bioz Stars, 2020-07
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Clone Assay:

Article Title: Engineering of CHO Cells for the Production of Recombinant Glycoprotein Vaccines with Xylosylated N-glycans
Article Snippet: .. The gene was cloned into the EcoRV restriction site of the vector pBGGPEX1 (ProBioGen AG, Berlin, Germany) by EcoRI/BamHI digestion, and followed by DNA polymerase Klenow (Roche, Mannheim, Germany) treatment resulting in the pBGGPEX1-RSV-F vector. ..

Plasmid Preparation:

Article Title: Engineering of CHO Cells for the Production of Recombinant Glycoprotein Vaccines with Xylosylated N-glycans
Article Snippet: .. The gene was cloned into the EcoRV restriction site of the vector pBGGPEX1 (ProBioGen AG, Berlin, Germany) by EcoRI/BamHI digestion, and followed by DNA polymerase Klenow (Roche, Mannheim, Germany) treatment resulting in the pBGGPEX1-RSV-F vector. ..

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  • 92
    Roche klenow fragment
    Force dependence of the replication rate. ( a ) Replication by <t>Klenow</t> on a charomid is started at 3 pN. Increasing the force to 24 pN stops the replication. After reducing the force back to 3 pN, the polymerization degree, N , has not changed and the enzyme starts replicating again. ( b ) Number of bases N ( t ) replicated by Sequenase on pXΔII at two different forces: 1 pN and 16 pN. ( c ) Mean replication rate, 〈 v 〉, versus force for Sequenase: high stringency hybridization ( K = 13, J = 154; ○) and random priming ( K = 5, J = 82; ⋄). The error bars represent σ 〈 v 〉 estimated as explained in Materials and Methods . The full curve is a fit to the model described in the text 〈 v ( F )〉 = v 0 exp(− nF Δ h / k B T ), where v 0 = 200 b/s and n = 2.1 (only ○ were fitted). The dashed curve is obtained with the previously determined v 0 and n = 1 (as expected if only one base was rate determining). ( d ) Replication rate versus force for Klenow: low stringency hybridization ( K = 24, J = 298; ⋄), high stringency hybridization ( K = 9, J = 114; ○). Replication rate for Klenow minus <t>exo3′→5′</t> high stringency hybridization ( K = 4, J = 50; □). The full curve is a fit to ⋄, where v 0 = 13.5 b/s and n = 4.
    Klenow Fragment, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/klenow fragment/product/Roche
    Average 92 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    klenow fragment - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    89
    Roche klenow fragment dna polymerase
    Translesion synthesis by Pol I. A. Translesion synthesis by E. coli <t>Klenow</t> fragment (2,5 nM) and Pol I (20 nM) on 34-mer templates harbouring a single lesion (X) at position 16. The polymerases have to add two nucleotides before encountering the lesion. oG = 8-oxoguanine; dHT = di-hydrothymidine; Tg = thymine-glycol and THF = tetra-hydrofurane, an abasic site analogue. The concentrations used for the two <t>DNA</t> polymerases were chosen for yielding the same activity on undamaged DNA. B. Nucleotide selection. Extension reactions directly on a lesion were carried out for 5 min in the presence of a single dNTP (0.1 mM) as indicated for each lane.
    Klenow Fragment Dna Polymerase, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/klenow fragment dna polymerase/product/Roche
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    klenow fragment dna polymerase - by Bioz Stars, 2020-07
    89/100 stars
      Buy from Supplier

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    Force dependence of the replication rate. ( a ) Replication by Klenow on a charomid is started at 3 pN. Increasing the force to 24 pN stops the replication. After reducing the force back to 3 pN, the polymerization degree, N , has not changed and the enzyme starts replicating again. ( b ) Number of bases N ( t ) replicated by Sequenase on pXΔII at two different forces: 1 pN and 16 pN. ( c ) Mean replication rate, 〈 v 〉, versus force for Sequenase: high stringency hybridization ( K = 13, J = 154; ○) and random priming ( K = 5, J = 82; ⋄). The error bars represent σ 〈 v 〉 estimated as explained in Materials and Methods . The full curve is a fit to the model described in the text 〈 v ( F )〉 = v 0 exp(− nF Δ h / k B T ), where v 0 = 200 b/s and n = 2.1 (only ○ were fitted). The dashed curve is obtained with the previously determined v 0 and n = 1 (as expected if only one base was rate determining). ( d ) Replication rate versus force for Klenow: low stringency hybridization ( K = 24, J = 298; ⋄), high stringency hybridization ( K = 9, J = 114; ○). Replication rate for Klenow minus exo3′→5′ high stringency hybridization ( K = 4, J = 50; □). The full curve is a fit to ⋄, where v 0 = 13.5 b/s and n = 4.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Replication by a single DNA polymerase of a stretched single-stranded DNA

    doi:

    Figure Lengend Snippet: Force dependence of the replication rate. ( a ) Replication by Klenow on a charomid is started at 3 pN. Increasing the force to 24 pN stops the replication. After reducing the force back to 3 pN, the polymerization degree, N , has not changed and the enzyme starts replicating again. ( b ) Number of bases N ( t ) replicated by Sequenase on pXΔII at two different forces: 1 pN and 16 pN. ( c ) Mean replication rate, 〈 v 〉, versus force for Sequenase: high stringency hybridization ( K = 13, J = 154; ○) and random priming ( K = 5, J = 82; ⋄). The error bars represent σ 〈 v 〉 estimated as explained in Materials and Methods . The full curve is a fit to the model described in the text 〈 v ( F )〉 = v 0 exp(− nF Δ h / k B T ), where v 0 = 200 b/s and n = 2.1 (only ○ were fitted). The dashed curve is obtained with the previously determined v 0 and n = 1 (as expected if only one base was rate determining). ( d ) Replication rate versus force for Klenow: low stringency hybridization ( K = 24, J = 298; ⋄), high stringency hybridization ( K = 9, J = 114; ○). Replication rate for Klenow minus exo3′→5′ high stringency hybridization ( K = 4, J = 50; □). The full curve is a fit to ⋄, where v 0 = 13.5 b/s and n = 4.

    Article Snippet: The capillary was then rinsed with 10 mM Tris (pH 8)/100 mM NaCl, after which Klenow fragment (Roche Molecular Biochemicals), Klenow fragment minus exo3′→5′ (New England Biolabs), or sequenase Version 2.0 (Amersham Pharmacia) was injected at ≈20 nM in the polymerization buffer.

    Techniques: Hybridization

    Time evolution of the replication on a pXΔII ssDNA under a tension of 1 pN (specific priming). ( a ) Number of bases N ( t ) replicated by Klenow minus exo3′→5′ as a function of time in two successive replication runs on the same template. Notice that pauses in replication occur at different positions in successive runs. The dots represents the raw data after low-pass filtering at ≈1 Hz. The extension of the DNA is converted into the number of added nucleotides N ( t ) as explained in the text. The full lines are polygon fits (see Materials and Methods ) with an average time duration of 100 s. ( b ) N ( t ) for Sequenase in two runs on different molecules. The full lines are polygon fits with an average time duration of 10 s.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Replication by a single DNA polymerase of a stretched single-stranded DNA

    doi:

    Figure Lengend Snippet: Time evolution of the replication on a pXΔII ssDNA under a tension of 1 pN (specific priming). ( a ) Number of bases N ( t ) replicated by Klenow minus exo3′→5′ as a function of time in two successive replication runs on the same template. Notice that pauses in replication occur at different positions in successive runs. The dots represents the raw data after low-pass filtering at ≈1 Hz. The extension of the DNA is converted into the number of added nucleotides N ( t ) as explained in the text. The full lines are polygon fits (see Materials and Methods ) with an average time duration of 100 s. ( b ) N ( t ) for Sequenase in two runs on different molecules. The full lines are polygon fits with an average time duration of 10 s.

    Article Snippet: The capillary was then rinsed with 10 mM Tris (pH 8)/100 mM NaCl, after which Klenow fragment (Roche Molecular Biochemicals), Klenow fragment minus exo3′→5′ (New England Biolabs), or sequenase Version 2.0 (Amersham Pharmacia) was injected at ≈20 nM in the polymerization buffer.

    Techniques:

    Strategy used to transfer cDNA from Gateway entry vectors to L. lactis vectors. A. Overview of the cloning procedure. In this strategy, recombination occurs between the att L sites from Gateway entry vectors (containing the cDNA coding for the proteins of interest) and the att R sites from a “destination” vector (att: attachment sites). This destination vector (pBS-RfA) is a derivative of pBlueScript which contains the reading frame cassette A (RfA cassette) surrounded by two Eco RV sites. By LR recombination, the first step thus generates a “shuttle” vector in which the gene of interest is surrounded by the two att B sites and two flanking Eco RV restriction sites. In order to generate blunt ends, the nisin inducible vector pNZ8148 is digested by Nco I and treated with the Klenow enzyme (pNZ8148NK). The cDNA excised from the “shuttle” vector pBS-RfA-cDNA with Eco RV (generating blunt ends) is ligated into the vector pNZ8148NK and placed under the control of the P nisA promoter. Positive selection of recombinant vectors is obtained using digestion of the ligation products with NsiI. B. Resulting N - and C - termini of the ORF. Nucleotide sequences at each side of the ORF (upper panel: 5′ORF and lower panel: 3′ORF). In bold, the ATG (start codon) and TAG (stop codon) of the cDNA. CCATG in bold corresponds to the Nco I site after digestion and treatment with the Klenow enzyme; the ATG within the Nco I site is in the reading frame of the ATG of the cDNA. The att B sites are in italics and the two half-sites resulting from Eco RV digestion are in bold italics.

    Journal: PLoS ONE

    Article Title: Lactococcus lactis, an Alternative System for Functional Expression of Peripheral and Intrinsic Arabidopsis Membrane Proteins

    doi: 10.1371/journal.pone.0008746

    Figure Lengend Snippet: Strategy used to transfer cDNA from Gateway entry vectors to L. lactis vectors. A. Overview of the cloning procedure. In this strategy, recombination occurs between the att L sites from Gateway entry vectors (containing the cDNA coding for the proteins of interest) and the att R sites from a “destination” vector (att: attachment sites). This destination vector (pBS-RfA) is a derivative of pBlueScript which contains the reading frame cassette A (RfA cassette) surrounded by two Eco RV sites. By LR recombination, the first step thus generates a “shuttle” vector in which the gene of interest is surrounded by the two att B sites and two flanking Eco RV restriction sites. In order to generate blunt ends, the nisin inducible vector pNZ8148 is digested by Nco I and treated with the Klenow enzyme (pNZ8148NK). The cDNA excised from the “shuttle” vector pBS-RfA-cDNA with Eco RV (generating blunt ends) is ligated into the vector pNZ8148NK and placed under the control of the P nisA promoter. Positive selection of recombinant vectors is obtained using digestion of the ligation products with NsiI. B. Resulting N - and C - termini of the ORF. Nucleotide sequences at each side of the ORF (upper panel: 5′ORF and lower panel: 3′ORF). In bold, the ATG (start codon) and TAG (stop codon) of the cDNA. CCATG in bold corresponds to the Nco I site after digestion and treatment with the Klenow enzyme; the ATG within the Nco I site is in the reading frame of the ATG of the cDNA. The att B sites are in italics and the two half-sites resulting from Eco RV digestion are in bold italics.

    Article Snippet: The three independent cDNAs were excised from the pBS-RfA-cDNA vector by digestion with Eco RV and ligated into pNZ8148NK. pNZ8148NK was obtained by digestion with Nco I and subsequent treatment by Klenow fragment (Roche, Switzerland) which filled the cohesive ends.

    Techniques: Clone Assay, Plasmid Preparation, Selection, Recombinant, Ligation

    Translesion synthesis by Pol I. A. Translesion synthesis by E. coli Klenow fragment (2,5 nM) and Pol I (20 nM) on 34-mer templates harbouring a single lesion (X) at position 16. The polymerases have to add two nucleotides before encountering the lesion. oG = 8-oxoguanine; dHT = di-hydrothymidine; Tg = thymine-glycol and THF = tetra-hydrofurane, an abasic site analogue. The concentrations used for the two DNA polymerases were chosen for yielding the same activity on undamaged DNA. B. Nucleotide selection. Extension reactions directly on a lesion were carried out for 5 min in the presence of a single dNTP (0.1 mM) as indicated for each lane.

    Journal: PLoS Genetics

    Article Title: Unexpected Role for Helicobacter pylori DNA Polymerase I As a Source of Genetic Variability

    doi: 10.1371/journal.pgen.1002152

    Figure Lengend Snippet: Translesion synthesis by Pol I. A. Translesion synthesis by E. coli Klenow fragment (2,5 nM) and Pol I (20 nM) on 34-mer templates harbouring a single lesion (X) at position 16. The polymerases have to add two nucleotides before encountering the lesion. oG = 8-oxoguanine; dHT = di-hydrothymidine; Tg = thymine-glycol and THF = tetra-hydrofurane, an abasic site analogue. The concentrations used for the two DNA polymerases were chosen for yielding the same activity on undamaged DNA. B. Nucleotide selection. Extension reactions directly on a lesion were carried out for 5 min in the presence of a single dNTP (0.1 mM) as indicated for each lane.

    Article Snippet: When required as a control, Klenow fragment DNA polymerase (Roche) was used.

    Techniques: Translesion Synthesis, Activity Assay, Selection