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Illumina Inc dna polymerase i
Dna Polymerase I, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dna polymerase i/product/Illumina Inc
Average 93 stars, based on 67 article reviews
Price from $9.99 to $1999.99
dna polymerase i - by Bioz Stars, 2020-08
93/100 stars

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Amplification:

Article Title: Aspartyl proteases in Candida glabrata are required for suppression of the host innate immune response
Article Snippet: .. The Superscript III reverse transcriptase and random hexamers were used for reverse transcription of RNA, and second-strand cDNA was synthesized in the presence of DNA polymerase I and RNase H. Following Illumina Adapter ligation to cDNA molecules, the library was amplified using eight cycles of PCR for enrichment of adapter-ligated fragments, and 76-base pair paired-end reads were produced with the Illumina NextSeq500. ..

Sample Prep:

Article Title: Uhrf1 regulates active transcriptional marks at bivalent domains in pluripotent stem cells through Setd1a
Article Snippet: .. First strand cDNA synthesis was done using the SuperScript III First-Strand Synthesis System and 3 μg μl−1 random hexamers (Invitrogen), followed by second strand synthesis with DNA Polymerase I and RNase H. After purification, a sequencing library was generated from the double-stranded cDNA by using paired-end adaptors (Illumina) and the NEBNext DNA Sample Prep Reagent Set 1 (NEB). .. This library was sequenced using an Illumina Genome HiSeq2000.

Ligation:

Article Title: Aspartyl proteases in Candida glabrata are required for suppression of the host innate immune response
Article Snippet: .. The Superscript III reverse transcriptase and random hexamers were used for reverse transcription of RNA, and second-strand cDNA was synthesized in the presence of DNA polymerase I and RNase H. Following Illumina Adapter ligation to cDNA molecules, the library was amplified using eight cycles of PCR for enrichment of adapter-ligated fragments, and 76-base pair paired-end reads were produced with the Illumina NextSeq500. ..

Synthesized:

Article Title: Aspartyl proteases in Candida glabrata are required for suppression of the host innate immune response
Article Snippet: .. The Superscript III reverse transcriptase and random hexamers were used for reverse transcription of RNA, and second-strand cDNA was synthesized in the presence of DNA polymerase I and RNase H. Following Illumina Adapter ligation to cDNA molecules, the library was amplified using eight cycles of PCR for enrichment of adapter-ligated fragments, and 76-base pair paired-end reads were produced with the Illumina NextSeq500. ..

Article Title: Conserved developmental transcriptomes in evolutionarily divergent species
Article Snippet: .. We then synthesized second strand cDNA with DNA Polymerase I and RNaseH in an Illumina custom buffer (Illumina, San Diego, CA, USA). .. We purified the products on a QiaQuick PCR column (Qiagen, Valencia, CA, USA) and eluted them in 30 μl EB buffer (Qiagen).

Article Title: Transcriptome Analysis of the Global Response of Pseudomonas fragi NMC25 to Modified Atmosphere Packaging Stress
Article Snippet: .. First strand cDNA was synthesized using random oligonucleotides and SuperScript III, followed by the synthesis of second strand cDNA using DNA polymerase I and RNase H. Exonuclease and polymerase were used to blunt and adenylate the 3’ ends of the DNA fragments, and Illumina PE adapter oligonucleotides were ligated to prepare for hybridization. .. To select cDNA fragments around 300 bp in length, the library fragments were purified using the AMPure XP system (Beckman Coulter, Beverly, CA, United States).

Article Title: Oxidative Stress Response of Aspergillus oryzae Induced by Hydrogen Peroxide and Menadione Sodium Bisulfite
Article Snippet: .. These fragments were then reverse transcripted into cDNA with random primers and the second-strand cDNA was synthesized using DNA polymerase I and RNase H. The resulting double-stranded cDNA was purified, end repaired and ligated to Illumina sequencing adapters. .. The cDNA sequencing was performed using Illumina HiSeq 2500 (Biomarker Biotechnology Co., Beijing, China).

Construct:

Article Title: Comparative transcriptome analysis of mammary epithelial cells at different stages of lactation reveals wide differences in gene expression and pathways regulating milk synthesis between Jersey and Kashmiri cattle
Article Snippet: .. The cleaved RNA fragments was used to synthesize first strand cDNA using reverse transcriptase and random primers (Illumina, USA) followed by second strand cDNA synthesis using DNA Polymerase I and RNase H. After adenylation of 3’ ends of DNA fragments, hybridisation was initiated by ligating Illumina paired-end adapter and index. cDNA fragments (200bp) were generated and were selectively enriched to construct the final sequencing paired end library using Illumina PCR Primer Cocktail. .. Libraries were pooled in equimolar amounts and paired end sequenced (126 bp) on three lanes (6/lane) on a High Throughput Model flow cell on an Illumina HiSeq 2500 platform by SciGenom, Cochin, Kerela-India.

Purification:

Article Title: Oxidative Stress Response of Aspergillus oryzae Induced by Hydrogen Peroxide and Menadione Sodium Bisulfite
Article Snippet: .. These fragments were then reverse transcripted into cDNA with random primers and the second-strand cDNA was synthesized using DNA polymerase I and RNase H. The resulting double-stranded cDNA was purified, end repaired and ligated to Illumina sequencing adapters. .. The cDNA sequencing was performed using Illumina HiSeq 2500 (Biomarker Biotechnology Co., Beijing, China).

Article Title: Uhrf1 regulates active transcriptional marks at bivalent domains in pluripotent stem cells through Setd1a
Article Snippet: .. First strand cDNA synthesis was done using the SuperScript III First-Strand Synthesis System and 3 μg μl−1 random hexamers (Invitrogen), followed by second strand synthesis with DNA Polymerase I and RNase H. After purification, a sequencing library was generated from the double-stranded cDNA by using paired-end adaptors (Illumina) and the NEBNext DNA Sample Prep Reagent Set 1 (NEB). .. This library was sequenced using an Illumina Genome HiSeq2000.

Produced:

Article Title: Aspartyl proteases in Candida glabrata are required for suppression of the host innate immune response
Article Snippet: .. The Superscript III reverse transcriptase and random hexamers were used for reverse transcription of RNA, and second-strand cDNA was synthesized in the presence of DNA polymerase I and RNase H. Following Illumina Adapter ligation to cDNA molecules, the library was amplified using eight cycles of PCR for enrichment of adapter-ligated fragments, and 76-base pair paired-end reads were produced with the Illumina NextSeq500. ..

Sequencing:

Article Title: Comparative transcriptome analysis of mammary epithelial cells at different stages of lactation reveals wide differences in gene expression and pathways regulating milk synthesis between Jersey and Kashmiri cattle
Article Snippet: .. The cleaved RNA fragments was used to synthesize first strand cDNA using reverse transcriptase and random primers (Illumina, USA) followed by second strand cDNA synthesis using DNA Polymerase I and RNase H. After adenylation of 3’ ends of DNA fragments, hybridisation was initiated by ligating Illumina paired-end adapter and index. cDNA fragments (200bp) were generated and were selectively enriched to construct the final sequencing paired end library using Illumina PCR Primer Cocktail. .. Libraries were pooled in equimolar amounts and paired end sequenced (126 bp) on three lanes (6/lane) on a High Throughput Model flow cell on an Illumina HiSeq 2500 platform by SciGenom, Cochin, Kerela-India.

Article Title: Uhrf1 regulates active transcriptional marks at bivalent domains in pluripotent stem cells through Setd1a
Article Snippet: .. First strand cDNA synthesis was done using the SuperScript III First-Strand Synthesis System and 3 μg μl−1 random hexamers (Invitrogen), followed by second strand synthesis with DNA Polymerase I and RNase H. After purification, a sequencing library was generated from the double-stranded cDNA by using paired-end adaptors (Illumina) and the NEBNext DNA Sample Prep Reagent Set 1 (NEB). .. This library was sequenced using an Illumina Genome HiSeq2000.

Generated:

Article Title: Comparative transcriptome analysis of mammary epithelial cells at different stages of lactation reveals wide differences in gene expression and pathways regulating milk synthesis between Jersey and Kashmiri cattle
Article Snippet: .. The cleaved RNA fragments was used to synthesize first strand cDNA using reverse transcriptase and random primers (Illumina, USA) followed by second strand cDNA synthesis using DNA Polymerase I and RNase H. After adenylation of 3’ ends of DNA fragments, hybridisation was initiated by ligating Illumina paired-end adapter and index. cDNA fragments (200bp) were generated and were selectively enriched to construct the final sequencing paired end library using Illumina PCR Primer Cocktail. .. Libraries were pooled in equimolar amounts and paired end sequenced (126 bp) on three lanes (6/lane) on a High Throughput Model flow cell on an Illumina HiSeq 2500 platform by SciGenom, Cochin, Kerela-India.

Article Title: Uhrf1 regulates active transcriptional marks at bivalent domains in pluripotent stem cells through Setd1a
Article Snippet: .. First strand cDNA synthesis was done using the SuperScript III First-Strand Synthesis System and 3 μg μl−1 random hexamers (Invitrogen), followed by second strand synthesis with DNA Polymerase I and RNase H. After purification, a sequencing library was generated from the double-stranded cDNA by using paired-end adaptors (Illumina) and the NEBNext DNA Sample Prep Reagent Set 1 (NEB). .. This library was sequenced using an Illumina Genome HiSeq2000.

Polymerase Chain Reaction:

Article Title: Comparative transcriptome analysis of mammary epithelial cells at different stages of lactation reveals wide differences in gene expression and pathways regulating milk synthesis between Jersey and Kashmiri cattle
Article Snippet: .. The cleaved RNA fragments was used to synthesize first strand cDNA using reverse transcriptase and random primers (Illumina, USA) followed by second strand cDNA synthesis using DNA Polymerase I and RNase H. After adenylation of 3’ ends of DNA fragments, hybridisation was initiated by ligating Illumina paired-end adapter and index. cDNA fragments (200bp) were generated and were selectively enriched to construct the final sequencing paired end library using Illumina PCR Primer Cocktail. .. Libraries were pooled in equimolar amounts and paired end sequenced (126 bp) on three lanes (6/lane) on a High Throughput Model flow cell on an Illumina HiSeq 2500 platform by SciGenom, Cochin, Kerela-India.

Article Title: Aspartyl proteases in Candida glabrata are required for suppression of the host innate immune response
Article Snippet: .. The Superscript III reverse transcriptase and random hexamers were used for reverse transcription of RNA, and second-strand cDNA was synthesized in the presence of DNA polymerase I and RNase H. Following Illumina Adapter ligation to cDNA molecules, the library was amplified using eight cycles of PCR for enrichment of adapter-ligated fragments, and 76-base pair paired-end reads were produced with the Illumina NextSeq500. ..

Hybridization:

Article Title: Comparative transcriptome analysis of mammary epithelial cells at different stages of lactation reveals wide differences in gene expression and pathways regulating milk synthesis between Jersey and Kashmiri cattle
Article Snippet: .. The cleaved RNA fragments was used to synthesize first strand cDNA using reverse transcriptase and random primers (Illumina, USA) followed by second strand cDNA synthesis using DNA Polymerase I and RNase H. After adenylation of 3’ ends of DNA fragments, hybridisation was initiated by ligating Illumina paired-end adapter and index. cDNA fragments (200bp) were generated and were selectively enriched to construct the final sequencing paired end library using Illumina PCR Primer Cocktail. .. Libraries were pooled in equimolar amounts and paired end sequenced (126 bp) on three lanes (6/lane) on a High Throughput Model flow cell on an Illumina HiSeq 2500 platform by SciGenom, Cochin, Kerela-India.

Article Title: Transcriptome Analysis of the Global Response of Pseudomonas fragi NMC25 to Modified Atmosphere Packaging Stress
Article Snippet: .. First strand cDNA was synthesized using random oligonucleotides and SuperScript III, followed by the synthesis of second strand cDNA using DNA polymerase I and RNase H. Exonuclease and polymerase were used to blunt and adenylate the 3’ ends of the DNA fragments, and Illumina PE adapter oligonucleotides were ligated to prepare for hybridization. .. To select cDNA fragments around 300 bp in length, the library fragments were purified using the AMPure XP system (Beckman Coulter, Beverly, CA, United States).

Article Title: Comparative transcriptome and metabolome provides new insights into the regulatory mechanisms of accelerated senescence in litchi fruit after cold storage
Article Snippet: .. The second-strand cDNA synthesis was performed using DNA polymerase I and ribonuclease H. After adenylation of the 3′-ends of the DNA fragments, Illumina PE adapter oligonucleotides were ligated to prepare for hybridisation. .. To preferentially select cDNA fragments of 200 base pairs (bp) in length, the library fragments were purified with the AMPure XP system (Beckman Coulter, Beverly, USA).

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  • 94
    Illumina Inc gdna
    <t>UBTF1/2</t> binds histone genes. ( A–C ) IGV (Integrated Genome Viewer) screenshots of mapped reads from UBTF1/2 ChIP and input <t>gDNA</t> at mouse histone gene cluster 1 ( A ), cluster 2 ( B ), and histone variant genes H2afx and H3f3a ( C ) in NIH3T3 cells. (
    Gdna, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 94/100, based on 537 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gdna/product/Illumina Inc
    Average 94 stars, based on 537 article reviews
    Price from $9.99 to $1999.99
    gdna - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    92
    Illumina Inc alu i digestion
    Schematic outline of the MB-seq and MRB-seq experiment. a Schematic drawing of MB-seq approach. Genomic DNA was randomly fragmented to 100–300 bp and ligated to Alu I-linkers with a methylated <t>Alu</t> I recognition site close to the T-overhang. The ligated-fragments were captured using methylcytosine antibody, then treated with sodium bisulfite and converted to double stranded DNA by amplification using biotin labelled Alu I primers. The Alu I-linker was digested and removed by streptavidin coupled beads. The linker-removed sequence was added 3’end A-tailing, then ligated to <t>Illumina</t> multiplexing adaptors following PCR amplification using Illumina paired-end PCR primers. The PCR products of 230–250 bases in length were size-selected on a gel and sequenced on the Illumina platform. b Schematic drawing of MRB-seq approach. Repetitive DNA elements were removed using Cot-1 DNA after MeDIP (based on the MB-seq approach). Cot-1 DNA was labelled with biotin and coupled with streptavidin, and the streptavidin-biotin-Cot-1 DNA was hybridized to enriched methylated DNA fragments via MeDIP to remove repeat fragments. The methylated fragments obtained (single/low copy DNA fragments) were then subjected to sodium bisulfite treatment, PCR amplification, Alu I digestion and sequencing library preparation, as per MB-seq
    Alu I Digestion, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alu i digestion/product/Illumina Inc
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    alu i digestion - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    93
    Illumina Inc dna polymerase i
    Schematic outline of the MB-seq and MRB-seq experiment. a Schematic drawing of MB-seq approach. Genomic DNA was randomly fragmented to 100–300 bp and ligated to Alu I-linkers with a methylated <t>Alu</t> I recognition site close to the T-overhang. The ligated-fragments were captured using methylcytosine antibody, then treated with sodium bisulfite and converted to double stranded DNA by amplification using biotin labelled Alu I primers. The Alu I-linker was digested and removed by streptavidin coupled beads. The linker-removed sequence was added 3’end A-tailing, then ligated to <t>Illumina</t> multiplexing adaptors following PCR amplification using Illumina paired-end PCR primers. The PCR products of 230–250 bases in length were size-selected on a gel and sequenced on the Illumina platform. b Schematic drawing of MRB-seq approach. Repetitive DNA elements were removed using Cot-1 DNA after MeDIP (based on the MB-seq approach). Cot-1 DNA was labelled with biotin and coupled with streptavidin, and the streptavidin-biotin-Cot-1 DNA was hybridized to enriched methylated DNA fragments via MeDIP to remove repeat fragments. The methylated fragments obtained (single/low copy DNA fragments) were then subjected to sodium bisulfite treatment, PCR amplification, Alu I digestion and sequencing library preparation, as per MB-seq
    Dna Polymerase I, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna polymerase i/product/Illumina Inc
    Average 93 stars, based on 67 article reviews
    Price from $9.99 to $1999.99
    dna polymerase i - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    Image Search Results


    UBTF1/2 binds histone genes. ( A–C ) IGV (Integrated Genome Viewer) screenshots of mapped reads from UBTF1/2 ChIP and input gDNA at mouse histone gene cluster 1 ( A ), cluster 2 ( B ), and histone variant genes H2afx and H3f3a ( C ) in NIH3T3 cells. (

    Journal: Genome Research

    Article Title: A novel role for the Pol I transcription factor UBTF in maintaining genome stability through the regulation of highly transcribed Pol II genes

    doi: 10.1101/gr.176115.114

    Figure Lengend Snippet: UBTF1/2 binds histone genes. ( A–C ) IGV (Integrated Genome Viewer) screenshots of mapped reads from UBTF1/2 ChIP and input gDNA at mouse histone gene cluster 1 ( A ), cluster 2 ( B ), and histone variant genes H2afx and H3f3a ( C ) in NIH3T3 cells. (

    Article Snippet: ChIP-seq of UBTF1/2 ChIP, Pol I, and gDNA samples in HMEC was performed using the Illumina Genome Analyzer II platform, while ChIP-seq of UBTF1/2 ChIP and gDNA samples in HMLER cells was performed using Illumina HiSeq 1000.

    Techniques: Chromatin Immunoprecipitation, Variant Assay

    Schematic outline of the MB-seq and MRB-seq experiment. a Schematic drawing of MB-seq approach. Genomic DNA was randomly fragmented to 100–300 bp and ligated to Alu I-linkers with a methylated Alu I recognition site close to the T-overhang. The ligated-fragments were captured using methylcytosine antibody, then treated with sodium bisulfite and converted to double stranded DNA by amplification using biotin labelled Alu I primers. The Alu I-linker was digested and removed by streptavidin coupled beads. The linker-removed sequence was added 3’end A-tailing, then ligated to Illumina multiplexing adaptors following PCR amplification using Illumina paired-end PCR primers. The PCR products of 230–250 bases in length were size-selected on a gel and sequenced on the Illumina platform. b Schematic drawing of MRB-seq approach. Repetitive DNA elements were removed using Cot-1 DNA after MeDIP (based on the MB-seq approach). Cot-1 DNA was labelled with biotin and coupled with streptavidin, and the streptavidin-biotin-Cot-1 DNA was hybridized to enriched methylated DNA fragments via MeDIP to remove repeat fragments. The methylated fragments obtained (single/low copy DNA fragments) were then subjected to sodium bisulfite treatment, PCR amplification, Alu I digestion and sequencing library preparation, as per MB-seq

    Journal: BMC Biotechnology

    Article Title: DNA methylome profiling at single-base resolution through bisulfite sequencing of 5mC-immunoprecipitated DNA

    doi: 10.1186/s12896-017-0409-7

    Figure Lengend Snippet: Schematic outline of the MB-seq and MRB-seq experiment. a Schematic drawing of MB-seq approach. Genomic DNA was randomly fragmented to 100–300 bp and ligated to Alu I-linkers with a methylated Alu I recognition site close to the T-overhang. The ligated-fragments were captured using methylcytosine antibody, then treated with sodium bisulfite and converted to double stranded DNA by amplification using biotin labelled Alu I primers. The Alu I-linker was digested and removed by streptavidin coupled beads. The linker-removed sequence was added 3’end A-tailing, then ligated to Illumina multiplexing adaptors following PCR amplification using Illumina paired-end PCR primers. The PCR products of 230–250 bases in length were size-selected on a gel and sequenced on the Illumina platform. b Schematic drawing of MRB-seq approach. Repetitive DNA elements were removed using Cot-1 DNA after MeDIP (based on the MB-seq approach). Cot-1 DNA was labelled with biotin and coupled with streptavidin, and the streptavidin-biotin-Cot-1 DNA was hybridized to enriched methylated DNA fragments via MeDIP to remove repeat fragments. The methylated fragments obtained (single/low copy DNA fragments) were then subjected to sodium bisulfite treatment, PCR amplification, Alu I digestion and sequencing library preparation, as per MB-seq

    Article Snippet: Library preparation and Illumina Solexa high-throughput sequencing Sequencing libraries of purified DNA from Alu I digestion were constructed following the Illumina paired ends sequencing library protocol (Illumine, USA).

    Techniques: Methylation, Amplification, Sequencing, Multiplexing, Polymerase Chain Reaction, Methylated DNA Immunoprecipitation