dna polymerase alpha  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Millipore dna polymerase alpha
    Dna Polymerase Alpha, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna polymerase alpha/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna polymerase alpha - by Bioz Stars, 2020-07
    99/100 stars

    Images

    Related Articles

    Negative Control:

    Article Title: SMAD2 Inactivation Inhibits CLDN6 Methylation to Suppress Migration and Invasion of Breast Cancer Cells
    Article Snippet: .. Antibodies included anti-DNMT1 (1:500, Cell Signaling Technology), anti-IgG (Millipore), and anti-DNA polymerase II (Millipore); anti-IgG was the negative control and anti-DNA polymerase II was the positive control. .. Transfection With Short Hairpin RNA Cells were transfected with short hairpin RNA (shRNA) by using Lipofectamine 2000 (Invitrogen), following the procedure recommended by the manufacturer. shRNA targeting of CLDN6 (5’-GTGCAAGGTGTACGACTCA-3’) and a negative control shRNA were purchased from GeneChem Co. Ltd.

    Amplification:

    Article Title: Molecular Characterization of Natural Erwinia pyrifoliae Strains Deficient in Hypersensitive Response
    Article Snippet: .. To reconfirm the hrpL sequences, DNA from strains Ep2/97, Ep4/97, and Ep16/96 was also amplified with a proofreading DNA polymerase (Accu Taq ; Sigma). .. The same sequence as in Fig. was obtained for the three wild-type strains, and the change from C to T at position 277 for strain Ep2/97 was observed.

    Positive Control:

    Article Title: SMAD2 Inactivation Inhibits CLDN6 Methylation to Suppress Migration and Invasion of Breast Cancer Cells
    Article Snippet: .. Antibodies included anti-DNMT1 (1:500, Cell Signaling Technology), anti-IgG (Millipore), and anti-DNA polymerase II (Millipore); anti-IgG was the negative control and anti-DNA polymerase II was the positive control. .. Transfection With Short Hairpin RNA Cells were transfected with short hairpin RNA (shRNA) by using Lipofectamine 2000 (Invitrogen), following the procedure recommended by the manufacturer. shRNA targeting of CLDN6 (5’-GTGCAAGGTGTACGACTCA-3’) and a negative control shRNA were purchased from GeneChem Co. Ltd.

    Activation Assay:

    Article Title: Transcriptomic profiling of human embryonic stem cells upon cell cycle manipulation during pluripotent state dissolution
    Article Snippet: .. 2.2 Experimental groups and conditions To understand the effects of DNA replication perturbation and activation of the DNA damage checkpoint in hESCs undergoing PSD, we supplemented hESCs either with DMSO, with the DNA polymerase inhibitor Aphidicolin (75 ng/mL, Sigma), or with Aphidicolin plus the checkpoint kinase inhibitor AZD7762 (100 nM, SelleckChem). .. To understand effects of Cyclin B1 overexpression in hESCs undergoing PSD, we infected hESCs either with the unmodified pLVTH vector or with the open reading frame of CCNB1 inserted using the PmeI and NdeI restriction sites.

    Labeling:

    Article Title: E2F1 Regulates the Base Excision Repair Gene XRCC1 and Promotes DNA Repair *
    Article Snippet: .. Cells were rinsed in phosphate-buffered saline, permeabilized with 1% Triton X-100 for 20 min, and then incubated in a moist air chamber at 37 °C for 90 min in a labeling mixture containing 10 μ m each dGTP, dATP, and dCTP and 7 μ m dTTP, 3 μ m FITC-dUTP, 20 units/ml Escherichia coli DNA polymerase I (Sigma) in reaction buffer containing 5 m m MgCl2 , 10 m m 2-mercaptoethanol, and 20 μg/ml bovine serum albumin. ..

    Concentration Assay:

    Article Title: The Heme Metabolite Carbon Monoxide Facilitates KSHV Infection by Inhibiting TLR4 Signaling in Endothelial Cells
    Article Snippet: .. The viral DNA polymerase inhibitor Foscarnet (FOS, Sigma) was used at a concentration of 400 μM. .. The CO releasing molecule CORM-2 (Sigma) was used at a concentration of 10 μM.

    Incubation:

    Article Title: E2F1 Regulates the Base Excision Repair Gene XRCC1 and Promotes DNA Repair *
    Article Snippet: .. Cells were rinsed in phosphate-buffered saline, permeabilized with 1% Triton X-100 for 20 min, and then incubated in a moist air chamber at 37 °C for 90 min in a labeling mixture containing 10 μ m each dGTP, dATP, and dCTP and 7 μ m dTTP, 3 μ m FITC-dUTP, 20 units/ml Escherichia coli DNA polymerase I (Sigma) in reaction buffer containing 5 m m MgCl2 , 10 m m 2-mercaptoethanol, and 20 μg/ml bovine serum albumin. ..

    Activity Assay:

    Article Title: Characterisation of Muta(TM)Mouse ?gt10-lacZ transgene: evidence for in vivo rearrangements
    Article Snippet: .. These custom-made primers (Cortec DNA Service Laboratories Inc., Kingston, Canada) were used to generate polymerase chain reaction (PCR) fragments in reactions involving a DNA polymerase deficient in 3′–5′ exonuclease activity (KOD polymerase; Novagen, Gibbstown, NJ, USA) to minimise proofreading errors. .. Fragments generated, either by PCR or cloning in E.coli , were sequenced in both directions using an ABI Prism® 3100 Genetic Analyzer and BigDye® Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA).

    Polymerase Chain Reaction:

    Article Title: Characterisation of Muta(TM)Mouse ?gt10-lacZ transgene: evidence for in vivo rearrangements
    Article Snippet: .. These custom-made primers (Cortec DNA Service Laboratories Inc., Kingston, Canada) were used to generate polymerase chain reaction (PCR) fragments in reactions involving a DNA polymerase deficient in 3′–5′ exonuclease activity (KOD polymerase; Novagen, Gibbstown, NJ, USA) to minimise proofreading errors. .. Fragments generated, either by PCR or cloning in E.coli , were sequenced in both directions using an ABI Prism® 3100 Genetic Analyzer and BigDye® Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore anti dna polymerase ii
    <t>DNA</t> methyltransferase 1 <t>(DNMT1)-mediated</t> upregulation of CLDN6 expression by SB431542. ( A , B ) Real-time polymerase chain reaction (RT-PCR) and immunoblot analysis of DNMT1 and CLDN6, and densitometric analysis of relative gene expression levels after normalization to loading controls GAPDH and β-actin are presented. ( C ) DNMT1 activity assays. ( D ) Methylation-specific PCR (MSP) analysis of CpG island of CLDN6 promoter using bisulfite-treated genomic DNA isolated from MCF-7 and SKBR-3 cells. ( E ) CpG island methylation within the CLDN6 promoter region was measured by bisulfite sequencing in SKBR-3 cells. “Me” stands for methylated, and “U” stands for unmethylated. ( F ) Chromatin immunoprecipitation-polymerase chain reaction (ChIP-PCR) assay to detect the binding of DNMT1 to the promoter of CLDN6 (** p
    Anti Dna Polymerase Ii, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti dna polymerase ii/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti dna polymerase ii - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    94
    Millipore ssea 1
    Effect of histone deacetylation on DNA methylation at PpG enhancers. ESC: undifferentiated embryonic stem cells, and ESC -LIF: E14T cells induced to differentiate, ESC -LIF+Dox: ZHBTc4 cells induced to differentiate. ( A ) ChIP-qPCR was used to determine the percent enrichments of H3K27ac at the PpG enhancers in ESC and cells 7 days post-induction of differentiation in absence and presence of Prg and/or C646, Prg: Pargyline, C646: p300 Histone acetyltransferase inhibitor. ( B ) Alkaline phosphatase staining (blue) and <t>SSEA-1</t> immunofluorescence (red) in ESCs and cells 9 days post induction of differentiation in absence and presence of C646 and C646 with pargyline, where positive staining indicates pluripotency. ( C ) ChIP-qPCR was used to determine the percent enrichments of H3K4me1 at the PpG enhancers in ESC and cells 7 days post-induction of differentiation in absence and presence of Prg and/or C646. ( D ) DNA methylation analysis of the PpG enhancers in ESCs and cells post induction of differentiation in absence and presence of Prg and/or C646 was assayed by MD-qPCR as in Figure 1A . (E and F) Bis-seq was used to determine the extent of CpG methylation at these enhancers in ( E ) E14T cells or ( F ) ZHBTc4 cells and the data were analyzed using Bismark software (details same as Figure 1B ) and the data are the average and SEM of 6 PpG enhancers shown in D. P -values are derived from Student's paired t -test. For A, C and D average and SD are shown for each gene.
    Ssea 1, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 102 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ssea 1/product/Millipore
    Average 94 stars, based on 102 article reviews
    Price from $9.99 to $1999.99
    ssea 1 - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    98
    Millipore cell apoptosis apoptosis
    Inhibition of miR-222 promotes erythrocyte lysate-induced microglia cell viability and reduces <t>apoptosis</t> by targeting ITGB8. (A) A reverse transcription-quantitative PCR assay was performed to assess the transfection efficiency. *P
    Cell Apoptosis Apoptosis, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell apoptosis apoptosis/product/Millipore
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cell apoptosis apoptosis - by Bioz Stars, 2020-07
    98/100 stars
      Buy from Supplier

    Image Search Results


    DNA methyltransferase 1 (DNMT1)-mediated upregulation of CLDN6 expression by SB431542. ( A , B ) Real-time polymerase chain reaction (RT-PCR) and immunoblot analysis of DNMT1 and CLDN6, and densitometric analysis of relative gene expression levels after normalization to loading controls GAPDH and β-actin are presented. ( C ) DNMT1 activity assays. ( D ) Methylation-specific PCR (MSP) analysis of CpG island of CLDN6 promoter using bisulfite-treated genomic DNA isolated from MCF-7 and SKBR-3 cells. ( E ) CpG island methylation within the CLDN6 promoter region was measured by bisulfite sequencing in SKBR-3 cells. “Me” stands for methylated, and “U” stands for unmethylated. ( F ) Chromatin immunoprecipitation-polymerase chain reaction (ChIP-PCR) assay to detect the binding of DNMT1 to the promoter of CLDN6 (** p

    Journal: International Journal of Molecular Sciences

    Article Title: SMAD2 Inactivation Inhibits CLDN6 Methylation to Suppress Migration and Invasion of Breast Cancer Cells

    doi: 10.3390/ijms18091863

    Figure Lengend Snippet: DNA methyltransferase 1 (DNMT1)-mediated upregulation of CLDN6 expression by SB431542. ( A , B ) Real-time polymerase chain reaction (RT-PCR) and immunoblot analysis of DNMT1 and CLDN6, and densitometric analysis of relative gene expression levels after normalization to loading controls GAPDH and β-actin are presented. ( C ) DNMT1 activity assays. ( D ) Methylation-specific PCR (MSP) analysis of CpG island of CLDN6 promoter using bisulfite-treated genomic DNA isolated from MCF-7 and SKBR-3 cells. ( E ) CpG island methylation within the CLDN6 promoter region was measured by bisulfite sequencing in SKBR-3 cells. “Me” stands for methylated, and “U” stands for unmethylated. ( F ) Chromatin immunoprecipitation-polymerase chain reaction (ChIP-PCR) assay to detect the binding of DNMT1 to the promoter of CLDN6 (** p

    Article Snippet: Antibodies included anti-DNMT1 (1:500, Cell Signaling Technology), anti-IgG (Millipore), and anti-DNA polymerase II (Millipore); anti-IgG was the negative control and anti-DNA polymerase II was the positive control.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Activity Assay, Methylation, Polymerase Chain Reaction, Isolation, Methylation Sequencing, Chromatin Immunoprecipitation, Binding Assay

    Effect of histone deacetylation on DNA methylation at PpG enhancers. ESC: undifferentiated embryonic stem cells, and ESC -LIF: E14T cells induced to differentiate, ESC -LIF+Dox: ZHBTc4 cells induced to differentiate. ( A ) ChIP-qPCR was used to determine the percent enrichments of H3K27ac at the PpG enhancers in ESC and cells 7 days post-induction of differentiation in absence and presence of Prg and/or C646, Prg: Pargyline, C646: p300 Histone acetyltransferase inhibitor. ( B ) Alkaline phosphatase staining (blue) and SSEA-1 immunofluorescence (red) in ESCs and cells 9 days post induction of differentiation in absence and presence of C646 and C646 with pargyline, where positive staining indicates pluripotency. ( C ) ChIP-qPCR was used to determine the percent enrichments of H3K4me1 at the PpG enhancers in ESC and cells 7 days post-induction of differentiation in absence and presence of Prg and/or C646. ( D ) DNA methylation analysis of the PpG enhancers in ESCs and cells post induction of differentiation in absence and presence of Prg and/or C646 was assayed by MD-qPCR as in Figure 1A . (E and F) Bis-seq was used to determine the extent of CpG methylation at these enhancers in ( E ) E14T cells or ( F ) ZHBTc4 cells and the data were analyzed using Bismark software (details same as Figure 1B ) and the data are the average and SEM of 6 PpG enhancers shown in D. P -values are derived from Student's paired t -test. For A, C and D average and SD are shown for each gene.

    Journal: Nucleic Acids Research

    Article Title: An epigenetic switch regulates de novo DNA methylation at a subset of pluripotency gene enhancers during embryonic stem cell differentiation

    doi: 10.1093/nar/gkw426

    Figure Lengend Snippet: Effect of histone deacetylation on DNA methylation at PpG enhancers. ESC: undifferentiated embryonic stem cells, and ESC -LIF: E14T cells induced to differentiate, ESC -LIF+Dox: ZHBTc4 cells induced to differentiate. ( A ) ChIP-qPCR was used to determine the percent enrichments of H3K27ac at the PpG enhancers in ESC and cells 7 days post-induction of differentiation in absence and presence of Prg and/or C646, Prg: Pargyline, C646: p300 Histone acetyltransferase inhibitor. ( B ) Alkaline phosphatase staining (blue) and SSEA-1 immunofluorescence (red) in ESCs and cells 9 days post induction of differentiation in absence and presence of C646 and C646 with pargyline, where positive staining indicates pluripotency. ( C ) ChIP-qPCR was used to determine the percent enrichments of H3K4me1 at the PpG enhancers in ESC and cells 7 days post-induction of differentiation in absence and presence of Prg and/or C646. ( D ) DNA methylation analysis of the PpG enhancers in ESCs and cells post induction of differentiation in absence and presence of Prg and/or C646 was assayed by MD-qPCR as in Figure 1A . (E and F) Bis-seq was used to determine the extent of CpG methylation at these enhancers in ( E ) E14T cells or ( F ) ZHBTc4 cells and the data were analyzed using Bismark software (details same as Figure 1B ) and the data are the average and SEM of 6 PpG enhancers shown in D. P -values are derived from Student's paired t -test. For A, C and D average and SD are shown for each gene.

    Article Snippet: Antibodies used for immunofluorescence include: SSEA-1 (Millipore, MAB430) 1:2000 and AlexaFluor 555 nm (Life Technologies, A21422) 1:1000.

    Techniques: DNA Methylation Assay, Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction, Staining, Immunofluorescence, CpG Methylation Assay, Software, Derivative Assay

    Lsd1 inhibition affects DNA methylation at PpG enhancers in absence of Oct4 expression. ESC: undifferentiated embryonic stem cells, and ESC -LIF +Dox: Cells induced to differentiate by Doxycycline treatment. ( A ) Percent enrichments of H3K4me1 using ChIP-qPCR at the PpG enhancers in ESC and cells 4 days post-induction of differentiation normalized to a negative control region in absence and presence of Lsd1 inhibitors, Prg: Pargyline, TCP: Tranylcypromine. ( B ) Alkaline phosphatase (blue stain) and SSEA-1 immunofluorescence staining (red stain) for pluripotency in ESCs and cells 8 days post-induction of differentiation in absence and presence of Prg, where positive staining indicates pluripotency. ( C ) Gene expression of PpGs by RT-qPCR in ZHBTc4 cells untreated and treated with Lsd1 inhibitors for 8 days post induction of differentiation. Gene expression is normalized to Gapdh and represented relative to ESC. ( D ) Average expression change with SEM across all loci shown in C; ( E ) DNA methylation analysis of the PpG enhancers in ESCs and cells 4 days post-induction of differentiation in absence and presence of pargyline and TCP was assayed by MD-qPCR as in Figure 1A . ( F ) Bis-seq was used to determine the extent of CpG methylation (details same as Figure 1B ) and the data are the average and SEM of 6 PpG enhancers shown in E. ( G ) Percent enrichment of Dnmt3a using ChIP-qPCR at PpG enhancers from cells pre- and 4 days post-induction of differentiation in presence and absence of prg. P -values are derived from Student's paired t -test using GraphPad Prism. For A, C, E and G average and SD are shown for each gene.

    Journal: Nucleic Acids Research

    Article Title: An epigenetic switch regulates de novo DNA methylation at a subset of pluripotency gene enhancers during embryonic stem cell differentiation

    doi: 10.1093/nar/gkw426

    Figure Lengend Snippet: Lsd1 inhibition affects DNA methylation at PpG enhancers in absence of Oct4 expression. ESC: undifferentiated embryonic stem cells, and ESC -LIF +Dox: Cells induced to differentiate by Doxycycline treatment. ( A ) Percent enrichments of H3K4me1 using ChIP-qPCR at the PpG enhancers in ESC and cells 4 days post-induction of differentiation normalized to a negative control region in absence and presence of Lsd1 inhibitors, Prg: Pargyline, TCP: Tranylcypromine. ( B ) Alkaline phosphatase (blue stain) and SSEA-1 immunofluorescence staining (red stain) for pluripotency in ESCs and cells 8 days post-induction of differentiation in absence and presence of Prg, where positive staining indicates pluripotency. ( C ) Gene expression of PpGs by RT-qPCR in ZHBTc4 cells untreated and treated with Lsd1 inhibitors for 8 days post induction of differentiation. Gene expression is normalized to Gapdh and represented relative to ESC. ( D ) Average expression change with SEM across all loci shown in C; ( E ) DNA methylation analysis of the PpG enhancers in ESCs and cells 4 days post-induction of differentiation in absence and presence of pargyline and TCP was assayed by MD-qPCR as in Figure 1A . ( F ) Bis-seq was used to determine the extent of CpG methylation (details same as Figure 1B ) and the data are the average and SEM of 6 PpG enhancers shown in E. ( G ) Percent enrichment of Dnmt3a using ChIP-qPCR at PpG enhancers from cells pre- and 4 days post-induction of differentiation in presence and absence of prg. P -values are derived from Student's paired t -test using GraphPad Prism. For A, C, E and G average and SD are shown for each gene.

    Article Snippet: Antibodies used for immunofluorescence include: SSEA-1 (Millipore, MAB430) 1:2000 and AlexaFluor 555 nm (Life Technologies, A21422) 1:1000.

    Techniques: Inhibition, DNA Methylation Assay, Expressing, Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction, Negative Control, Staining, Immunofluorescence, Quantitative RT-PCR, CpG Methylation Assay, Derivative Assay

    Lsd1 inhibition restricts establishment of DNA methylation at PpG enhancers by Dnmt3a. ESC: undifferentiated embryonic stem cells, and ESC-LIF: Cells induced to differentiate. ( A ) ChIP-qPCR was used to determine the percent enrichments of H3K4me1 at the PpG enhancers in ESC and cells 7 days post- induction of differentiation in absence and presence of Lsd1 inhibitors, Prg: Pargyline, TCP: Tranylcypromine. ( B ) Alkaline phosphatase staining (blue) and SSEA-1 immunofluorescence (red) in ESCs and cells 9 days post induction of differentiation in absence and presence of pargyline, where positive staining indicates pluripotency. ( C ) Gene expression analysis of PpGs using RT-qPCR in ESCs and cells 9 days post induction of differentiation in absence and presence of Lsd1 inhibitors. Gene expression is normalized to Gapdh and represented relative to ESC. ( D ) Average expression change with SEM across all loci shown in C; ( E ) DNA methylation analysis of the PpG enhancers in ESCs and cells 5 days post induction of differentiation in absence and presence of pargyline and TCP was assayed by MD-qPCR as in Figure 1A . ( F ) Bis-seq was used to determine the extent of CpG methylation (details same as Figure 1B ) and the data are the average and SEM of 6 PpG enhancers shown in E. ( G ) Percent enrichment of Dnmt3a using ChIP-qPCR at PpG enhancers in ESC and cells 7 days post-induction of differentiation in absence and presence of Lsd1 inhibitors. For D and F, P -values are derived from Student's paired t -test using GraphPad Prism. For A, C, E and G average and SD are shown for each gene.

    Journal: Nucleic Acids Research

    Article Title: An epigenetic switch regulates de novo DNA methylation at a subset of pluripotency gene enhancers during embryonic stem cell differentiation

    doi: 10.1093/nar/gkw426

    Figure Lengend Snippet: Lsd1 inhibition restricts establishment of DNA methylation at PpG enhancers by Dnmt3a. ESC: undifferentiated embryonic stem cells, and ESC-LIF: Cells induced to differentiate. ( A ) ChIP-qPCR was used to determine the percent enrichments of H3K4me1 at the PpG enhancers in ESC and cells 7 days post- induction of differentiation in absence and presence of Lsd1 inhibitors, Prg: Pargyline, TCP: Tranylcypromine. ( B ) Alkaline phosphatase staining (blue) and SSEA-1 immunofluorescence (red) in ESCs and cells 9 days post induction of differentiation in absence and presence of pargyline, where positive staining indicates pluripotency. ( C ) Gene expression analysis of PpGs using RT-qPCR in ESCs and cells 9 days post induction of differentiation in absence and presence of Lsd1 inhibitors. Gene expression is normalized to Gapdh and represented relative to ESC. ( D ) Average expression change with SEM across all loci shown in C; ( E ) DNA methylation analysis of the PpG enhancers in ESCs and cells 5 days post induction of differentiation in absence and presence of pargyline and TCP was assayed by MD-qPCR as in Figure 1A . ( F ) Bis-seq was used to determine the extent of CpG methylation (details same as Figure 1B ) and the data are the average and SEM of 6 PpG enhancers shown in E. ( G ) Percent enrichment of Dnmt3a using ChIP-qPCR at PpG enhancers in ESC and cells 7 days post-induction of differentiation in absence and presence of Lsd1 inhibitors. For D and F, P -values are derived from Student's paired t -test using GraphPad Prism. For A, C, E and G average and SD are shown for each gene.

    Article Snippet: Antibodies used for immunofluorescence include: SSEA-1 (Millipore, MAB430) 1:2000 and AlexaFluor 555 nm (Life Technologies, A21422) 1:1000.

    Techniques: Inhibition, DNA Methylation Assay, Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction, Staining, Immunofluorescence, Expressing, Quantitative RT-PCR, CpG Methylation Assay, Derivative Assay

    Inhibition of miR-222 promotes erythrocyte lysate-induced microglia cell viability and reduces apoptosis by targeting ITGB8. (A) A reverse transcription-quantitative PCR assay was performed to assess the transfection efficiency. *P

    Journal: Molecular Medicine Reports

    Article Title: miR-222 regulates brain injury and inflammation following intracerebral hemorrhage by targeting ITGB8

    doi: 10.3892/mmr.2019.10903

    Figure Lengend Snippet: Inhibition of miR-222 promotes erythrocyte lysate-induced microglia cell viability and reduces apoptosis by targeting ITGB8. (A) A reverse transcription-quantitative PCR assay was performed to assess the transfection efficiency. *P

    Article Snippet: Cell apoptosis Apoptosis was evaluated using an apoptosis and necrosis assay kit (Oncogene Research Products).

    Techniques: Inhibition, Real-time Polymerase Chain Reaction, Transfection

    miR-222 regulates erythrocyte lysate-induced microglia cell viability and apoptosis. (A) Reverse transcription-quantitative PCR assay was performed to assess the transfection efficiency. (B) Cell viability was detected in erythrocyte lysate-induced microglia by a Cell Counting Kit-8 assay. (C) Effect of miR-222 on cell apoptosis was evaluated by flow cytometric analysis. (D) Levels of apoptosis-related proteins including cleaved caspase-3, cleaved caspase-9, Bax and Bcl-2 were evaluated by western blotting. *P

    Journal: Molecular Medicine Reports

    Article Title: miR-222 regulates brain injury and inflammation following intracerebral hemorrhage by targeting ITGB8

    doi: 10.3892/mmr.2019.10903

    Figure Lengend Snippet: miR-222 regulates erythrocyte lysate-induced microglia cell viability and apoptosis. (A) Reverse transcription-quantitative PCR assay was performed to assess the transfection efficiency. (B) Cell viability was detected in erythrocyte lysate-induced microglia by a Cell Counting Kit-8 assay. (C) Effect of miR-222 on cell apoptosis was evaluated by flow cytometric analysis. (D) Levels of apoptosis-related proteins including cleaved caspase-3, cleaved caspase-9, Bax and Bcl-2 were evaluated by western blotting. *P

    Article Snippet: Cell apoptosis Apoptosis was evaluated using an apoptosis and necrosis assay kit (Oncogene Research Products).

    Techniques: Real-time Polymerase Chain Reaction, Transfection, Cell Counting, Western Blot