Structured Review

Agilent technologies dna microarray scanner g2565ba
Cancer cell AGR2/CD10 RNA expression levels. Levels were determined through <t>DNA</t> <t>microarray</t> analysis with signal intensity values on the y -axis. Indicated on the x -axis are G3 and G4 primary tumor cell types, L luminal, LNCaP, C4-2, CL1, DU145, PC3 cell lines, LuCaP35, LuCaP 49 xenografts. Except for DU145 and LuCaP 49, the expression pattern is either CD10 or AGR2.
Dna Microarray Scanner G2565ba, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dna microarray scanner g2565ba/product/Agilent technologies
Average 99 stars, based on 21 article reviews
Price from $9.99 to $1999.99
dna microarray scanner g2565ba - by Bioz Stars, 2020-03
99/100 stars

Images

1) Product Images from "Prostate cancer cell phenotypes based on AGR2 and CD10 expression"

Article Title: Prostate cancer cell phenotypes based on AGR2 and CD10 expression

Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

doi: 10.1038/modpathol.2012.238

Cancer cell AGR2/CD10 RNA expression levels. Levels were determined through DNA microarray analysis with signal intensity values on the y -axis. Indicated on the x -axis are G3 and G4 primary tumor cell types, L luminal, LNCaP, C4-2, CL1, DU145, PC3 cell lines, LuCaP35, LuCaP 49 xenografts. Except for DU145 and LuCaP 49, the expression pattern is either CD10 or AGR2.
Figure Legend Snippet: Cancer cell AGR2/CD10 RNA expression levels. Levels were determined through DNA microarray analysis with signal intensity values on the y -axis. Indicated on the x -axis are G3 and G4 primary tumor cell types, L luminal, LNCaP, C4-2, CL1, DU145, PC3 cell lines, LuCaP35, LuCaP 49 xenografts. Except for DU145 and LuCaP 49, the expression pattern is either CD10 or AGR2.

Techniques Used: RNA Expression, Microarray, Expressing

Expression levels of AGR2 and CD10 in xenografts. As inferred from DNA microarray analysis, these levels are presented in histogram format (top). CY3 intensity values are indicated on the y -axis. Because of the lower values for CD10, a separate histogram for CD10 is included (bottom). Note the ~6-fold decrease in CD10 from LuCaP 96 to Lu CaP 96CR.
Figure Legend Snippet: Expression levels of AGR2 and CD10 in xenografts. As inferred from DNA microarray analysis, these levels are presented in histogram format (top). CY3 intensity values are indicated on the y -axis. Because of the lower values for CD10, a separate histogram for CD10 is included (bottom). Note the ~6-fold decrease in CD10 from LuCaP 96 to Lu CaP 96CR.

Techniques Used: Expressing, Microarray

Related Articles

Amplification:

Article Title: The retinoblastoma tumor suppressor controls androgen signaling and human prostate cancer progression
Article Snippet: Freshly frozen samples of 22 LuCaP PCa xenografts were processed as described previously ( ) to extract total RNA, which was amplified 1 round and hybridized to Agilent 44K whole human genome expression oligonucleotide microarrays (Agilent Technologies). .. Probe labeling and hybridization was performed following the Agilent-suggested protocols, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA.

Article Title: PPP2R2C loss promotes castration-resistance and is associated with increased prostate cancer-specific mortality
Article Snippet: The tumor samples were laser-capture microdissected, and total RNA isolated and amplified as described previously( ). .. Probe labeling and hybridization was performed following the Agilent suggested protocols and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA.

Article Title: Androgen Receptor Promotes Ligand-Independent Prostate Cancer Progression through c-Myc Upregulation
Article Snippet: The tumor samples were laser-capture microdissected, and total RNA was isolated and amplified as described previously . .. Probe labeling and hybridization were performed following the Agilent suggested protocols, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA.

Article Title: IL-6 promotes prostate tumorigenesis and progression through autocrine cross-activation of IGF-IR
Article Snippet: Microarray analysis Total RNA was extracted as above. mRNA was amplified using the Ambion MessageAmp II Amplification Kit (Ambion Inc.), labeled, and hybridized to Agilent 44K whole human genome expression oligonucleotide microarray slides following manufacturer’s protocols (Agilent Technologies, Inc.). .. Fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA.

Article Title: SRRM4 Expression and the Loss of REST Activity May Promote the Emergence of the Neuroendocrine Phenotype in Castration-Resistant Prostate Cancer
Article Snippet: Paragraph title: RNA amplification and microarray hybridization ... Fluorescent array images were digitized using the Agilent DNA microarray scanner G2565BA.

Article Title: Prostate cancer cell phenotypes based on AGR2 and CD10 expression
Article Snippet: For array analysis, tumor (xenograft and metastasis) RNA was amplified one round and hybridized to Agilent 44K whole human genome expression oligonucleotide microarrays (Agilent Technologies, Santa Clara, CA). .. Probe labeling and hybridization was performed following the Agilent protocol, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA.

Article Title: Role of WNT7B-induced Noncanonical Pathway in Advanced Prostate Cancer
Article Snippet: For gene expression from prostate cancer xenograft tumors, RNA was extracted from 24 LuCaP prostate cancer xenografts as described previously ( ) and amplified and hybridized to Agilent 44K whole human genome expression oligonucleotide microarrays (Agilent Technologies). .. Probe labeling and hybridization were conducted following the Agilent suggested protocols, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA.

Fluorescence:

Article Title: Transcriptomic Analysis of Streptomyces coelicolor Differentiation in Solid Sporulating Cultures: First Compartmentalized and Second Multinucleated Mycelia Have Different and Distinctive Transcriptomes
Article Snippet: .. The slide was then briefly immersed in the Agilent Stabilization and Drying Solution prior to fluorescence reading with an Agilent DNA Microarray Scanner G2565BA using the extended dynamic mode. .. Quantification of the fluorescence intensities were performed using the FeatureExtraction software (v9.5.1, Agilent).

Article Title: Transcriptomic Analysis of Liquid Non-Sporulating Streptomyces coelicolor Cultures Demonstrates the Existence of a Complex Differentiation Comparable to That Occurring in Solid Sporulating Cultures
Article Snippet: .. The slide was then briefly immersed in the Agilent Stabilization and Drying Solution prior to measuring fluorescence with an Agilent DNA Microarray Scanner G2565BA using the extended dynamic mode. .. Quantification of fluorescence intensities was performed using the FeatureExtraction software (v9.5.1, Agilent).

Isolation:

Article Title: PPP2R2C loss promotes castration-resistance and is associated with increased prostate cancer-specific mortality
Article Snippet: The tumor samples were laser-capture microdissected, and total RNA isolated and amplified as described previously( ). .. Probe labeling and hybridization was performed following the Agilent suggested protocols and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA.

Article Title: Androgen Receptor Promotes Ligand-Independent Prostate Cancer Progression through c-Myc Upregulation
Article Snippet: The tumor samples were laser-capture microdissected, and total RNA was isolated and amplified as described previously . .. Probe labeling and hybridization were performed following the Agilent suggested protocols, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA.

Article Title: Transcriptomic Analysis of Streptomyces coelicolor Differentiation in Solid Sporulating Cultures: First Compartmentalized and Second Multinucleated Mycelia Have Different and Distinctive Transcriptomes
Article Snippet: Paragraph title: RNA isolation and microarray hybridization ... The slide was then briefly immersed in the Agilent Stabilization and Drying Solution prior to fluorescence reading with an Agilent DNA Microarray Scanner G2565BA using the extended dynamic mode.

Article Title: Transcriptomic Analysis of Liquid Non-Sporulating Streptomyces coelicolor Cultures Demonstrates the Existence of a Complex Differentiation Comparable to That Occurring in Solid Sporulating Cultures
Article Snippet: Paragraph title: 2.3. RNA Isolation and Microarray Hybridization ... The slide was then briefly immersed in the Agilent Stabilization and Drying Solution prior to measuring fluorescence with an Agilent DNA Microarray Scanner G2565BA using the extended dynamic mode.

Labeling:

Article Title: The retinoblastoma tumor suppressor controls androgen signaling and human prostate cancer progression
Article Snippet: .. Probe labeling and hybridization was performed following the Agilent-suggested protocols, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA. .. Agilent Feature Extraction software was used to grid, extract, and normalize data.

Article Title: PPP2R2C loss promotes castration-resistance and is associated with increased prostate cancer-specific mortality
Article Snippet: .. Probe labeling and hybridization was performed following the Agilent suggested protocols and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA. .. Agilent Feature Extraction software was used to grid, extract, and normalize data.

Article Title: Overexpression of IL-7 enhances cisplatin resistance in glioma
Article Snippet: The purified labeled samples were mixed with human Cot-1 DNA, denatured at 95°C (Oligo aCGH hybridization kit), and applied to microarrays. .. The hybridization was conducted at 65°C for 40 h. The microarrays were then washed in Oligo aCGH wash buffer 1 at room temperature for 5 min and wash buffer 2 at 37°C for 1 min. After drying, the microarrays were scanned using a DNA microarray scanner G2565BA (Agilent), and the data (log2 ) were extracted from raw microarray image files using Feature Extraction software (version 9; Agilent).

Article Title: Androgen Receptor Promotes Ligand-Independent Prostate Cancer Progression through c-Myc Upregulation
Article Snippet: .. Probe labeling and hybridization were performed following the Agilent suggested protocols, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA. .. Expression ratios were normalized using the R software Bioconductor snm package and combined with expression profiles from 15 normal prostate specimens to create z-scores, which represent the number of standard deviations away from the mean of expression in the normal prostate group , .

Article Title: Epithelial mesenchymal-like transition occurs in a subset of cells in castration resistant prostate cancer bone metastases
Article Snippet: .. Probe labeling and hybridization was performed following the Agilent suggested protocols and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA. .. TGFB1 transcript levels were measured using quantitative reverse transcription-PCR (qRT-PCR) in triplicate reactions using 5 ng first strand cDNA, 0.2 μmol/L of each primer, and Power SYBR Green PCR master mix (Applied Biosystems).

Article Title: IL-6 promotes prostate tumorigenesis and progression through autocrine cross-activation of IGF-IR
Article Snippet: Microarray analysis Total RNA was extracted as above. mRNA was amplified using the Ambion MessageAmp II Amplification Kit (Ambion Inc.), labeled, and hybridized to Agilent 44K whole human genome expression oligonucleotide microarray slides following manufacturer’s protocols (Agilent Technologies, Inc.). .. Fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA.

Article Title: SRRM4 Expression and the Loss of REST Activity May Promote the Emergence of the Neuroendocrine Phenotype in Castration-Resistant Prostate Cancer
Article Snippet: Probe labeling and hybridization followed Agilent protocols. .. Fluorescent array images were digitized using the Agilent DNA microarray scanner G2565BA.

Article Title: The chemokine CXCL13 in lung cancers associated with environmental polycyclic aromatic hydrocarbons pollution
Article Snippet: Total RNA (1 µg) was labeled and hybridized with a One-ColorQuick Amp Labeling Kit and Gene Expression Hybridization Kit (Agilent Technologies, Santa Clara, CA). .. Hybridization signals were detected using a DNA microarray scanner G2565BA (Agilent Technologies), and all scanned images were analyzed using Agilent Feature Extraction Software.

Article Title: Prostate cancer cell phenotypes based on AGR2 and CD10 expression
Article Snippet: .. Probe labeling and hybridization was performed following the Agilent protocol, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA. .. Agilent Feature Extraction software was used to grid, extract, and normalize data as reported in ref. .

Article Title: Transcriptomic Analysis of Liquid Non-Sporulating Streptomyces coelicolor Cultures Demonstrates the Existence of a Complex Differentiation Comparable to That Occurring in Solid Sporulating Cultures
Article Snippet: Genomic DNA from S. coelicolor M145 was used as the common reference. gDNA was labeled with Cy5-dCTP (GE Healthcare Life Sciences) using the BioPrime Array CGH Genomic Labeling Module (Invitrogen), purified with the MinElute kit, and labeling efficiencies quantified with a NanoDrop ND-1000 spectrophotometer. .. The slide was then briefly immersed in the Agilent Stabilization and Drying Solution prior to measuring fluorescence with an Agilent DNA Microarray Scanner G2565BA using the extended dynamic mode.

Article Title: Role of WNT7B-induced Noncanonical Pathway in Advanced Prostate Cancer
Article Snippet: .. Probe labeling and hybridization were conducted following the Agilent suggested protocols, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA. .. Agilent Feature Extraction Software was used to grid, extract, and normalize data.

Purification:

Article Title: Overexpression of IL-7 enhances cisplatin resistance in glioma
Article Snippet: The purified labeled samples were mixed with human Cot-1 DNA, denatured at 95°C (Oligo aCGH hybridization kit), and applied to microarrays. .. The hybridization was conducted at 65°C for 40 h. The microarrays were then washed in Oligo aCGH wash buffer 1 at room temperature for 5 min and wash buffer 2 at 37°C for 1 min. After drying, the microarrays were scanned using a DNA microarray scanner G2565BA (Agilent), and the data (log2 ) were extracted from raw microarray image files using Feature Extraction software (version 9; Agilent).

Article Title: ArgR of Streptomyces coelicolor Is a Versatile Regulator
Article Snippet: The final products were purified with MinElute columns (Qiagen) and labelling efficiencies were quantified spectrophotometrically. .. Washing, scanning with an Agilent DNA Microarray Scanner G2565BA, and image quantification were carried out as indicated previously .

Article Title: Transcriptomic analysis of Streptomyces clavuligerus ΔccaR::tsr: effects of the cephamycin C-clavulanic acid cluster regulator CcaR on global regulation *
Article Snippet: The final products were purified with MinElute columns (Qiagen, Venlo, the Netherlands) and labelling efficiencies were quantified spectrophotometrically. .. Washing, scanning with an Agilent DNA Microarray Scanner G2565BA and image quantification were carried out as indicated previously (Rodríguez-García et al ., ).

Article Title: Transcriptomic Analysis of Liquid Non-Sporulating Streptomyces coelicolor Cultures Demonstrates the Existence of a Complex Differentiation Comparable to That Occurring in Solid Sporulating Cultures
Article Snippet: Genomic DNA from S. coelicolor M145 was used as the common reference. gDNA was labeled with Cy5-dCTP (GE Healthcare Life Sciences) using the BioPrime Array CGH Genomic Labeling Module (Invitrogen), purified with the MinElute kit, and labeling efficiencies quantified with a NanoDrop ND-1000 spectrophotometer. .. The slide was then briefly immersed in the Agilent Stabilization and Drying Solution prior to measuring fluorescence with an Agilent DNA Microarray Scanner G2565BA using the extended dynamic mode.

Microarray:

Article Title: The retinoblastoma tumor suppressor controls androgen signaling and human prostate cancer progression
Article Snippet: .. Probe labeling and hybridization was performed following the Agilent-suggested protocols, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA. .. Agilent Feature Extraction software was used to grid, extract, and normalize data.

Article Title: PPP2R2C loss promotes castration-resistance and is associated with increased prostate cancer-specific mortality
Article Snippet: .. Probe labeling and hybridization was performed following the Agilent suggested protocols and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA. .. Agilent Feature Extraction software was used to grid, extract, and normalize data.

Article Title: Overexpression of IL-7 enhances cisplatin resistance in glioma
Article Snippet: .. The hybridization was conducted at 65°C for 40 h. The microarrays were then washed in Oligo aCGH wash buffer 1 at room temperature for 5 min and wash buffer 2 at 37°C for 1 min. After drying, the microarrays were scanned using a DNA microarray scanner G2565BA (Agilent), and the data (log2 ) were extracted from raw microarray image files using Feature Extraction software (version 9; Agilent). .. The data were analyzed using DNA analytics software (version 4.0; Agilent) with default filter settings.

Article Title: ArgR of Streptomyces coelicolor Is a Versatile Regulator
Article Snippet: .. Washing, scanning with an Agilent DNA Microarray Scanner G2565BA, and image quantification were carried out as indicated previously . .. Identification of differentially transcribed genes and transcription profile classification Microarray data were normalized and analysed with the Bioconductor package limma , .

Article Title: Androgen Receptor Promotes Ligand-Independent Prostate Cancer Progression through c-Myc Upregulation
Article Snippet: .. Probe labeling and hybridization were performed following the Agilent suggested protocols, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA. .. Expression ratios were normalized using the R software Bioconductor snm package and combined with expression profiles from 15 normal prostate specimens to create z-scores, which represent the number of standard deviations away from the mean of expression in the normal prostate group , .

Article Title: Epithelial mesenchymal-like transition occurs in a subset of cells in castration resistant prostate cancer bone metastases
Article Snippet: .. Probe labeling and hybridization was performed following the Agilent suggested protocols and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA. .. TGFB1 transcript levels were measured using quantitative reverse transcription-PCR (qRT-PCR) in triplicate reactions using 5 ng first strand cDNA, 0.2 μmol/L of each primer, and Power SYBR Green PCR master mix (Applied Biosystems).

Article Title: RNA isolation method for single embryo transcriptome analysis in zebrafish
Article Snippet: .. Afterwards the slides were washed and scanned in an ozone-free room with the Agilent DNA microarray scanner G2565BA (Agilent Technologies). ..

Article Title: Transcriptomic analysis of Streptomyces clavuligerus ΔccaR::tsr: effects of the cephamycin C-clavulanic acid cluster regulator CcaR on global regulation *
Article Snippet: .. Washing, scanning with an Agilent DNA Microarray Scanner G2565BA and image quantification were carried out as indicated previously (Rodríguez-García et al ., ). .. Identification of differentially transcribed genes and transcription profile classification Microarray data were normalized and analyzed with the Bioconductor package limma (Smyth and Speed, ; Smyth, ).

Article Title: IL-6 promotes prostate tumorigenesis and progression through autocrine cross-activation of IGF-IR
Article Snippet: .. Fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA. .. Data was analyzed using Agilent Feature Extraction software.

Article Title: SRRM4 Expression and the Loss of REST Activity May Promote the Emergence of the Neuroendocrine Phenotype in Castration-Resistant Prostate Cancer
Article Snippet: .. Fluorescent array images were digitized using the Agilent DNA microarray scanner G2565BA. ..

Article Title: The chemokine CXCL13 in lung cancers associated with environmental polycyclic aromatic hydrocarbons pollution
Article Snippet: .. Hybridization signals were detected using a DNA microarray scanner G2565BA (Agilent Technologies), and all scanned images were analyzed using Agilent Feature Extraction Software. .. Quantile normalization and subsequent data processing were performed using the GeneSpring GX v11.5.1 software package (Agilent Technologies).

Article Title: Prostate cancer cell phenotypes based on AGR2 and CD10 expression
Article Snippet: .. Probe labeling and hybridization was performed following the Agilent protocol, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA. .. Agilent Feature Extraction software was used to grid, extract, and normalize data as reported in ref. .

Article Title: Transcriptomic Analysis of Streptomyces coelicolor Differentiation in Solid Sporulating Cultures: First Compartmentalized and Second Multinucleated Mycelia Have Different and Distinctive Transcriptomes
Article Snippet: .. The slide was then briefly immersed in the Agilent Stabilization and Drying Solution prior to fluorescence reading with an Agilent DNA Microarray Scanner G2565BA using the extended dynamic mode. .. Quantification of the fluorescence intensities were performed using the FeatureExtraction software (v9.5.1, Agilent).

Article Title: Transcriptomic Analysis of Liquid Non-Sporulating Streptomyces coelicolor Cultures Demonstrates the Existence of a Complex Differentiation Comparable to That Occurring in Solid Sporulating Cultures
Article Snippet: .. The slide was then briefly immersed in the Agilent Stabilization and Drying Solution prior to measuring fluorescence with an Agilent DNA Microarray Scanner G2565BA using the extended dynamic mode. .. Quantification of fluorescence intensities was performed using the FeatureExtraction software (v9.5.1, Agilent).

Article Title: Role of WNT7B-induced Noncanonical Pathway in Advanced Prostate Cancer
Article Snippet: .. Probe labeling and hybridization were conducted following the Agilent suggested protocols, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA. .. Agilent Feature Extraction Software was used to grid, extract, and normalize data.

Spectrophotometry:

Article Title: Transcriptomic Analysis of Liquid Non-Sporulating Streptomyces coelicolor Cultures Demonstrates the Existence of a Complex Differentiation Comparable to That Occurring in Solid Sporulating Cultures
Article Snippet: Genomic DNA from S. coelicolor M145 was used as the common reference. gDNA was labeled with Cy5-dCTP (GE Healthcare Life Sciences) using the BioPrime Array CGH Genomic Labeling Module (Invitrogen), purified with the MinElute kit, and labeling efficiencies quantified with a NanoDrop ND-1000 spectrophotometer. .. The slide was then briefly immersed in the Agilent Stabilization and Drying Solution prior to measuring fluorescence with an Agilent DNA Microarray Scanner G2565BA using the extended dynamic mode.

Expressing:

Article Title: The retinoblastoma tumor suppressor controls androgen signaling and human prostate cancer progression
Article Snippet: Freshly frozen samples of 22 LuCaP PCa xenografts were processed as described previously ( ) to extract total RNA, which was amplified 1 round and hybridized to Agilent 44K whole human genome expression oligonucleotide microarrays (Agilent Technologies). .. Probe labeling and hybridization was performed following the Agilent-suggested protocols, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA.

Article Title: PPP2R2C loss promotes castration-resistance and is associated with increased prostate cancer-specific mortality
Article Snippet: Paragraph title: Gene Expression Microarrays ... Probe labeling and hybridization was performed following the Agilent suggested protocols and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA.

Article Title: Androgen Receptor Promotes Ligand-Independent Prostate Cancer Progression through c-Myc Upregulation
Article Snippet: Paragraph title: AR and c-Myc Expression in Human Castration-Resistant Prostate Cancer Tumors ... Probe labeling and hybridization were performed following the Agilent suggested protocols, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA.

Article Title: Epithelial mesenchymal-like transition occurs in a subset of cells in castration resistant prostate cancer bone metastases
Article Snippet: Agilent 44K whole human genome expression oligonucleotide microarrays (Agilent Technologies Inc) were used. .. Probe labeling and hybridization was performed following the Agilent suggested protocols and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA.

Article Title: RNA isolation method for single embryo transcriptome analysis in zebrafish
Article Snippet: Microarray hybridization, scanning & data processing Each hybridization mixture was made up from 300 ng 'Test' and 300 ng 'Reference' sample according to the Two-Color Microarray-Based Gene Expression Analysis Manual version 5.5 (Agilent Technologies). .. Afterwards the slides were washed and scanned in an ozone-free room with the Agilent DNA microarray scanner G2565BA (Agilent Technologies).

Article Title: IL-6 promotes prostate tumorigenesis and progression through autocrine cross-activation of IGF-IR
Article Snippet: Microarray analysis Total RNA was extracted as above. mRNA was amplified using the Ambion MessageAmp II Amplification Kit (Ambion Inc.), labeled, and hybridized to Agilent 44K whole human genome expression oligonucleotide microarray slides following manufacturer’s protocols (Agilent Technologies, Inc.). .. Fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA.

Article Title: SRRM4 Expression and the Loss of REST Activity May Promote the Emergence of the Neuroendocrine Phenotype in Castration-Resistant Prostate Cancer
Article Snippet: Agilent 44K whole human genome expression oligonucleotide microarrays (Agilent Technologies, Inc.) were used to profile the PCa xenograft lines and CRPC metastases. .. Fluorescent array images were digitized using the Agilent DNA microarray scanner G2565BA.

Article Title: The chemokine CXCL13 in lung cancers associated with environmental polycyclic aromatic hydrocarbons pollution
Article Snippet: Total RNA (1 µg) was labeled and hybridized with a One-ColorQuick Amp Labeling Kit and Gene Expression Hybridization Kit (Agilent Technologies, Santa Clara, CA). .. Hybridization signals were detected using a DNA microarray scanner G2565BA (Agilent Technologies), and all scanned images were analyzed using Agilent Feature Extraction Software.

Article Title: Prostate cancer cell phenotypes based on AGR2 and CD10 expression
Article Snippet: Paragraph title: Expression levels from array signal values ... Probe labeling and hybridization was performed following the Agilent protocol, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA.

Article Title: Transcriptomic Analysis of Streptomyces coelicolor Differentiation in Solid Sporulating Cultures: First Compartmentalized and Second Multinucleated Mycelia Have Different and Distinctive Transcriptomes
Article Snippet: The slide was washed in 50 ml of Agilent Gene Expression Wash Buffer 1 for 5 min at room temperature, and then in 50 ml of Wash Buffer 2 preheated at 37°C for 1 min. .. The slide was then briefly immersed in the Agilent Stabilization and Drying Solution prior to fluorescence reading with an Agilent DNA Microarray Scanner G2565BA using the extended dynamic mode.

Article Title: Transcriptomic Analysis of Liquid Non-Sporulating Streptomyces coelicolor Cultures Demonstrates the Existence of a Complex Differentiation Comparable to That Occurring in Solid Sporulating Cultures
Article Snippet: The slide was washed in 50 ml of Agilent Gene Expression Wash Buffer 1 for 5 min at room temperature and then in 50 ml of Wash Buffer 2 preheated to 37°C for 1 min, both with horizontal agitation (85 rpm). .. The slide was then briefly immersed in the Agilent Stabilization and Drying Solution prior to measuring fluorescence with an Agilent DNA Microarray Scanner G2565BA using the extended dynamic mode.

Article Title: Role of WNT7B-induced Noncanonical Pathway in Advanced Prostate Cancer
Article Snippet: For gene expression from prostate cancer xenograft tumors, RNA was extracted from 24 LuCaP prostate cancer xenografts as described previously ( ) and amplified and hybridized to Agilent 44K whole human genome expression oligonucleotide microarrays (Agilent Technologies). .. Probe labeling and hybridization were conducted following the Agilent suggested protocols, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA.

Software:

Article Title: The retinoblastoma tumor suppressor controls androgen signaling and human prostate cancer progression
Article Snippet: Probe labeling and hybridization was performed following the Agilent-suggested protocols, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA. .. Agilent Feature Extraction software was used to grid, extract, and normalize data.

Article Title: PPP2R2C loss promotes castration-resistance and is associated with increased prostate cancer-specific mortality
Article Snippet: Probe labeling and hybridization was performed following the Agilent suggested protocols and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA. .. Agilent Feature Extraction software was used to grid, extract, and normalize data.

Article Title: Overexpression of IL-7 enhances cisplatin resistance in glioma
Article Snippet: .. The hybridization was conducted at 65°C for 40 h. The microarrays were then washed in Oligo aCGH wash buffer 1 at room temperature for 5 min and wash buffer 2 at 37°C for 1 min. After drying, the microarrays were scanned using a DNA microarray scanner G2565BA (Agilent), and the data (log2 ) were extracted from raw microarray image files using Feature Extraction software (version 9; Agilent). .. The data were analyzed using DNA analytics software (version 4.0; Agilent) with default filter settings.

Article Title: Androgen Receptor Promotes Ligand-Independent Prostate Cancer Progression through c-Myc Upregulation
Article Snippet: Probe labeling and hybridization were performed following the Agilent suggested protocols, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA. .. Expression ratios were normalized using the R software Bioconductor snm package and combined with expression profiles from 15 normal prostate specimens to create z-scores, which represent the number of standard deviations away from the mean of expression in the normal prostate group , .

Article Title: RNA isolation method for single embryo transcriptome analysis in zebrafish
Article Snippet: Afterwards the slides were washed and scanned in an ozone-free room with the Agilent DNA microarray scanner G2565BA (Agilent Technologies). .. Microarray data was extracted with Feature Extraction Software version 9.5.3 (Agilent Technologies).

Article Title: IL-6 promotes prostate tumorigenesis and progression through autocrine cross-activation of IGF-IR
Article Snippet: Fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA. .. Data was analyzed using Agilent Feature Extraction software.

Article Title: SRRM4 Expression and the Loss of REST Activity May Promote the Emergence of the Neuroendocrine Phenotype in Castration-Resistant Prostate Cancer
Article Snippet: Fluorescent array images were digitized using the Agilent DNA microarray scanner G2565BA. .. Control probes were removed, duplicate probes averaged, and spots flagged by Agilent Feature Extraction software as being foreground feature nonuniformity or population outliers were assigned a value of "NA".

Article Title: The chemokine CXCL13 in lung cancers associated with environmental polycyclic aromatic hydrocarbons pollution
Article Snippet: .. Hybridization signals were detected using a DNA microarray scanner G2565BA (Agilent Technologies), and all scanned images were analyzed using Agilent Feature Extraction Software. .. Quantile normalization and subsequent data processing were performed using the GeneSpring GX v11.5.1 software package (Agilent Technologies).

Article Title: Prostate cancer cell phenotypes based on AGR2 and CD10 expression
Article Snippet: Probe labeling and hybridization was performed following the Agilent protocol, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA. .. Agilent Feature Extraction software was used to grid, extract, and normalize data as reported in ref. .

Article Title: Transcriptomic Analysis of Streptomyces coelicolor Differentiation in Solid Sporulating Cultures: First Compartmentalized and Second Multinucleated Mycelia Have Different and Distinctive Transcriptomes
Article Snippet: The slide was then briefly immersed in the Agilent Stabilization and Drying Solution prior to fluorescence reading with an Agilent DNA Microarray Scanner G2565BA using the extended dynamic mode. .. Quantification of the fluorescence intensities were performed using the FeatureExtraction software (v9.5.1, Agilent).

Article Title: Transcriptomic Analysis of Liquid Non-Sporulating Streptomyces coelicolor Cultures Demonstrates the Existence of a Complex Differentiation Comparable to That Occurring in Solid Sporulating Cultures
Article Snippet: The slide was then briefly immersed in the Agilent Stabilization and Drying Solution prior to measuring fluorescence with an Agilent DNA Microarray Scanner G2565BA using the extended dynamic mode. .. Quantification of fluorescence intensities was performed using the FeatureExtraction software (v9.5.1, Agilent).

Article Title: Role of WNT7B-induced Noncanonical Pathway in Advanced Prostate Cancer
Article Snippet: Probe labeling and hybridization were conducted following the Agilent suggested protocols, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA. .. Agilent Feature Extraction Software was used to grid, extract, and normalize data.

Hybridization:

Article Title: The retinoblastoma tumor suppressor controls androgen signaling and human prostate cancer progression
Article Snippet: .. Probe labeling and hybridization was performed following the Agilent-suggested protocols, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA. .. Agilent Feature Extraction software was used to grid, extract, and normalize data.

Article Title: PPP2R2C loss promotes castration-resistance and is associated with increased prostate cancer-specific mortality
Article Snippet: .. Probe labeling and hybridization was performed following the Agilent suggested protocols and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA. .. Agilent Feature Extraction software was used to grid, extract, and normalize data.

Article Title: Overexpression of IL-7 enhances cisplatin resistance in glioma
Article Snippet: .. The hybridization was conducted at 65°C for 40 h. The microarrays were then washed in Oligo aCGH wash buffer 1 at room temperature for 5 min and wash buffer 2 at 37°C for 1 min. After drying, the microarrays were scanned using a DNA microarray scanner G2565BA (Agilent), and the data (log2 ) were extracted from raw microarray image files using Feature Extraction software (version 9; Agilent). .. The data were analyzed using DNA analytics software (version 4.0; Agilent) with default filter settings.

Article Title: ArgR of Streptomyces coelicolor Is a Versatile Regulator
Article Snippet: Long Oligo/cDNA Hybridization Solution (Corning), to be applied on the microarray surface. .. Washing, scanning with an Agilent DNA Microarray Scanner G2565BA, and image quantification were carried out as indicated previously .

Article Title: Androgen Receptor Promotes Ligand-Independent Prostate Cancer Progression through c-Myc Upregulation
Article Snippet: .. Probe labeling and hybridization were performed following the Agilent suggested protocols, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA. .. Expression ratios were normalized using the R software Bioconductor snm package and combined with expression profiles from 15 normal prostate specimens to create z-scores, which represent the number of standard deviations away from the mean of expression in the normal prostate group , .

Article Title: Epithelial mesenchymal-like transition occurs in a subset of cells in castration resistant prostate cancer bone metastases
Article Snippet: .. Probe labeling and hybridization was performed following the Agilent suggested protocols and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA. .. TGFB1 transcript levels were measured using quantitative reverse transcription-PCR (qRT-PCR) in triplicate reactions using 5 ng first strand cDNA, 0.2 μmol/L of each primer, and Power SYBR Green PCR master mix (Applied Biosystems).

Article Title: RNA isolation method for single embryo transcriptome analysis in zebrafish
Article Snippet: Paragraph title: Microarray hybridization, scanning & data processing ... Afterwards the slides were washed and scanned in an ozone-free room with the Agilent DNA microarray scanner G2565BA (Agilent Technologies).

Article Title: Transcriptomic analysis of Streptomyces clavuligerus ΔccaR::tsr: effects of the cephamycin C-clavulanic acid cluster regulator CcaR on global regulation *
Article Snippet: Cy3-cDNA (300 ng) and Cy5-labelled gDNA (10 pmol) were mixed, vacuum dried, resuspended in 32 μL of hybridization solution (Agilent) and applied on the microarray surface. .. Washing, scanning with an Agilent DNA Microarray Scanner G2565BA and image quantification were carried out as indicated previously (Rodríguez-García et al ., ).

Article Title: SRRM4 Expression and the Loss of REST Activity May Promote the Emergence of the Neuroendocrine Phenotype in Castration-Resistant Prostate Cancer
Article Snippet: Paragraph title: RNA amplification and microarray hybridization ... Fluorescent array images were digitized using the Agilent DNA microarray scanner G2565BA.

Article Title: The chemokine CXCL13 in lung cancers associated with environmental polycyclic aromatic hydrocarbons pollution
Article Snippet: .. Hybridization signals were detected using a DNA microarray scanner G2565BA (Agilent Technologies), and all scanned images were analyzed using Agilent Feature Extraction Software. .. Quantile normalization and subsequent data processing were performed using the GeneSpring GX v11.5.1 software package (Agilent Technologies).

Article Title: Prostate cancer cell phenotypes based on AGR2 and CD10 expression
Article Snippet: .. Probe labeling and hybridization was performed following the Agilent protocol, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA. .. Agilent Feature Extraction software was used to grid, extract, and normalize data as reported in ref. .

Article Title: Transcriptomic Analysis of Streptomyces coelicolor Differentiation in Solid Sporulating Cultures: First Compartmentalized and Second Multinucleated Mycelia Have Different and Distinctive Transcriptomes
Article Snippet: Paragraph title: RNA isolation and microarray hybridization ... The slide was then briefly immersed in the Agilent Stabilization and Drying Solution prior to fluorescence reading with an Agilent DNA Microarray Scanner G2565BA using the extended dynamic mode.

Article Title: Transcriptomic Analysis of Liquid Non-Sporulating Streptomyces coelicolor Cultures Demonstrates the Existence of a Complex Differentiation Comparable to That Occurring in Solid Sporulating Cultures
Article Snippet: Paragraph title: 2.3. RNA Isolation and Microarray Hybridization ... The slide was then briefly immersed in the Agilent Stabilization and Drying Solution prior to measuring fluorescence with an Agilent DNA Microarray Scanner G2565BA using the extended dynamic mode.

Article Title: Role of WNT7B-induced Noncanonical Pathway in Advanced Prostate Cancer
Article Snippet: .. Probe labeling and hybridization were conducted following the Agilent suggested protocols, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA. .. Agilent Feature Extraction Software was used to grid, extract, and normalize data.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Agilent technologies dna microarray scanner
    Effects of CNT-1 and CNT-2 on gene expression in NR8383 cells. Venn diagram showing the number of upregulated and downregulated genes in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h (A). Heat map generated from <t>DNA</t> <t>microarray</t> data reflecting differential expression of genes involved in the response to stimulus (GO: 0050896) in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h. Expression levels are colored blue for low intensity and red for high intensity (see scale at the bottom right corner) (B).
    Dna Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 1473 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna microarray scanner/product/Agilent technologies
    Average 99 stars, based on 1473 article reviews
    Price from $9.99 to $1999.99
    dna microarray scanner - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    99
    Agilent technologies microarray scanner
    Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the <t>microarray</t> data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.
    Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarray scanner/product/Agilent technologies
    Average 99 stars, based on 105 article reviews
    Price from $9.99 to $1999.99
    microarray scanner - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    99
    Agilent technologies g2565ca dna microarray scanner
    Chromosome aneuploidy in L. infantum Sb2000.1 and Sb4000.4 antimony-resistant mutants. Log 2 -transformed Sb2000.1/WT expression ratios of the upregulated chromosome 1 ( A ), chromosome 11 ( B ) and chromosome 25 ( C ) and of the downregulated chromosome 9 ( D ), chromosome 12 ( E ) and chromosome 32 ( F ) plotted as a function of the location of <t>microarray</t> probes on each chromosomes. For each plot, the log 2 -transformed gene expression ratios of chromosome 30, which was equally expressed in both samples, are shown as a control (grey line). Quantitative Southern blot hybridizations of digested genomic <t>DNA</t> extracted from L. infantum WT (lane 1), Sb2000.1 (lane 2) and Sb4000.4 (lane 3) were performed to correlate gene expression modulation of entire chromosomes with the chromosome DNA copy number. The hybridization signal of LinJ30_V3.2990 was used for normalization. The hybridization signals were quantified using ImageQuant 5.2 (Molecular Dynamics) and the fold differences in DNA copy number of Sb2000.1 compared to WT are found below each blot.
    G2565ca Dna Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/g2565ca dna microarray scanner/product/Agilent technologies
    Average 99 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    g2565ca dna microarray scanner - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    99
    Agilent technologies dna microarray scanner g2565ba
    Cancer cell AGR2/CD10 RNA expression levels. Levels were determined through <t>DNA</t> <t>microarray</t> analysis with signal intensity values on the y -axis. Indicated on the x -axis are G3 and G4 primary tumor cell types, L luminal, LNCaP, C4-2, CL1, DU145, PC3 cell lines, LuCaP35, LuCaP 49 xenografts. Except for DU145 and LuCaP 49, the expression pattern is either CD10 or AGR2.
    Dna Microarray Scanner G2565ba, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna microarray scanner g2565ba/product/Agilent technologies
    Average 99 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    dna microarray scanner g2565ba - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    Effects of CNT-1 and CNT-2 on gene expression in NR8383 cells. Venn diagram showing the number of upregulated and downregulated genes in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h (A). Heat map generated from DNA microarray data reflecting differential expression of genes involved in the response to stimulus (GO: 0050896) in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h. Expression levels are colored blue for low intensity and red for high intensity (see scale at the bottom right corner) (B).

    Journal: Inhalation Toxicology

    Article Title: Size effects of single-walled carbon nanotubes on in vivo and in vitro pulmonary toxicity

    doi: 10.3109/08958378.2015.1026620

    Figure Lengend Snippet: Effects of CNT-1 and CNT-2 on gene expression in NR8383 cells. Venn diagram showing the number of upregulated and downregulated genes in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h (A). Heat map generated from DNA microarray data reflecting differential expression of genes involved in the response to stimulus (GO: 0050896) in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h. Expression levels are colored blue for low intensity and red for high intensity (see scale at the bottom right corner) (B).

    Article Snippet: Each labeled cRNA probe was used separately for hybridization to a 4 × 44 K Whole Rat Genome Microarray Kit (G4131F; Agilent Technologies, Santa Clara, CA), and hybridization was performed at 65 °C for 17 h. Hybridized microarray slides were washed according to the instructions of the manufacturer and were scanned with an Agilent DNA Microarray Scanner (G2565BA; Agilent Technologies, Santa Clara, CA) at 5-μm resolution.

    Techniques: Expressing, Generated, Microarray

    Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the microarray data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.

    Journal: Scientific Reports

    Article Title: Transcriptomics in lung tissue upon respiratory syncytial virus infection reveals aging as important modulator of immune activation and matrix maintenance

    doi: 10.1038/s41598-018-35180-2

    Figure Lengend Snippet: Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the microarray data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.

    Article Snippet: After washing (NimbleGen Arrays User’s Guide – Gene Expression Arrays Version 5.0), slides were scanned in an ozone-free room with a microarray scanner (Agilent DNA microarray scanner G2565CA, Agilent Technologies).

    Techniques: Expressing, Microarray, Derivative Assay, Mouse Assay

    Chromosome aneuploidy in L. infantum Sb2000.1 and Sb4000.4 antimony-resistant mutants. Log 2 -transformed Sb2000.1/WT expression ratios of the upregulated chromosome 1 ( A ), chromosome 11 ( B ) and chromosome 25 ( C ) and of the downregulated chromosome 9 ( D ), chromosome 12 ( E ) and chromosome 32 ( F ) plotted as a function of the location of microarray probes on each chromosomes. For each plot, the log 2 -transformed gene expression ratios of chromosome 30, which was equally expressed in both samples, are shown as a control (grey line). Quantitative Southern blot hybridizations of digested genomic DNA extracted from L. infantum WT (lane 1), Sb2000.1 (lane 2) and Sb4000.4 (lane 3) were performed to correlate gene expression modulation of entire chromosomes with the chromosome DNA copy number. The hybridization signal of LinJ30_V3.2990 was used for normalization. The hybridization signals were quantified using ImageQuant 5.2 (Molecular Dynamics) and the fold differences in DNA copy number of Sb2000.1 compared to WT are found below each blot.

    Journal: Nucleic Acids Research

    Article Title: Gene expression modulation is associated with gene amplification, supernumerary chromosomes and chromosome loss in antimony-resistant Leishmania infantum

    doi: 10.1093/nar/gkn1069

    Figure Lengend Snippet: Chromosome aneuploidy in L. infantum Sb2000.1 and Sb4000.4 antimony-resistant mutants. Log 2 -transformed Sb2000.1/WT expression ratios of the upregulated chromosome 1 ( A ), chromosome 11 ( B ) and chromosome 25 ( C ) and of the downregulated chromosome 9 ( D ), chromosome 12 ( E ) and chromosome 32 ( F ) plotted as a function of the location of microarray probes on each chromosomes. For each plot, the log 2 -transformed gene expression ratios of chromosome 30, which was equally expressed in both samples, are shown as a control (grey line). Quantitative Southern blot hybridizations of digested genomic DNA extracted from L. infantum WT (lane 1), Sb2000.1 (lane 2) and Sb4000.4 (lane 3) were performed to correlate gene expression modulation of entire chromosomes with the chromosome DNA copy number. The hybridization signal of LinJ30_V3.2990 was used for normalization. The hybridization signals were quantified using ImageQuant 5.2 (Molecular Dynamics) and the fold differences in DNA copy number of Sb2000.1 compared to WT are found below each blot.

    Article Snippet: Microarray data acquisition and analysis Detection of the Alexa Fluor 555 and Alexa Fluor 647 signals was performed on a G2565CA DNA microarray scanner (Agilent technologies) at a 5-μm resolution.

    Techniques: Transformation Assay, Expressing, Microarray, Southern Blot, Hybridization

    Cancer cell AGR2/CD10 RNA expression levels. Levels were determined through DNA microarray analysis with signal intensity values on the y -axis. Indicated on the x -axis are G3 and G4 primary tumor cell types, L luminal, LNCaP, C4-2, CL1, DU145, PC3 cell lines, LuCaP35, LuCaP 49 xenografts. Except for DU145 and LuCaP 49, the expression pattern is either CD10 or AGR2.

    Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

    Article Title: Prostate cancer cell phenotypes based on AGR2 and CD10 expression

    doi: 10.1038/modpathol.2012.238

    Figure Lengend Snippet: Cancer cell AGR2/CD10 RNA expression levels. Levels were determined through DNA microarray analysis with signal intensity values on the y -axis. Indicated on the x -axis are G3 and G4 primary tumor cell types, L luminal, LNCaP, C4-2, CL1, DU145, PC3 cell lines, LuCaP35, LuCaP 49 xenografts. Except for DU145 and LuCaP 49, the expression pattern is either CD10 or AGR2.

    Article Snippet: Probe labeling and hybridization was performed following the Agilent protocol, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA.

    Techniques: RNA Expression, Microarray, Expressing

    Expression levels of AGR2 and CD10 in xenografts. As inferred from DNA microarray analysis, these levels are presented in histogram format (top). CY3 intensity values are indicated on the y -axis. Because of the lower values for CD10, a separate histogram for CD10 is included (bottom). Note the ~6-fold decrease in CD10 from LuCaP 96 to Lu CaP 96CR.

    Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

    Article Title: Prostate cancer cell phenotypes based on AGR2 and CD10 expression

    doi: 10.1038/modpathol.2012.238

    Figure Lengend Snippet: Expression levels of AGR2 and CD10 in xenografts. As inferred from DNA microarray analysis, these levels are presented in histogram format (top). CY3 intensity values are indicated on the y -axis. Because of the lower values for CD10, a separate histogram for CD10 is included (bottom). Note the ~6-fold decrease in CD10 from LuCaP 96 to Lu CaP 96CR.

    Article Snippet: Probe labeling and hybridization was performed following the Agilent protocol, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA.

    Techniques: Expressing, Microarray