Structured Review

TaKaRa dna manipulation
Identification of rBacmid- APSL by <t>PCR.</t> The PCR product was electrophoresed on 1% agarose gel. Lane 1: 250 bp <t>DNA</t> ladder marker; Lane 2: PCR product of rBacmid- APSL with M13 F/M13 R; Lane 3: negative control (PCR product of wild-type bacmid with M13 F/M13 R).
Dna Manipulation, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "A Shark Liver Gene-Derived Active Peptide Expressed in the Silkworm, Bombyx mori: Preliminary Studies for Oral Administration of the Recombinant Protein"

Article Title: A Shark Liver Gene-Derived Active Peptide Expressed in the Silkworm, Bombyx mori: Preliminary Studies for Oral Administration of the Recombinant Protein

Journal: Marine Drugs

doi: 10.3390/md11051492

Identification of rBacmid- APSL by PCR. The PCR product was electrophoresed on 1% agarose gel. Lane 1: 250 bp DNA ladder marker; Lane 2: PCR product of rBacmid- APSL with M13 F/M13 R; Lane 3: negative control (PCR product of wild-type bacmid with M13 F/M13 R).
Figure Legend Snippet: Identification of rBacmid- APSL by PCR. The PCR product was electrophoresed on 1% agarose gel. Lane 1: 250 bp DNA ladder marker; Lane 2: PCR product of rBacmid- APSL with M13 F/M13 R; Lane 3: negative control (PCR product of wild-type bacmid with M13 F/M13 R).

Techniques Used: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Marker, Negative Control

Identification of recombinant virus rBv- APSL DNA by PCR. The PCR product was electrophoresed on 1% agarose gel. Lane 1: GeneRuler™ 1 kb DNA Ladder; Lane 2: PCR product of rBv-APSL with P1/P2; Lane 3: PCR product of rBv- APSL with P1/M13 R; Lane 4: PCR product of rBv- APSL with M13 F/P2; Lane 5: PCR product of rBv- APSL with M13 F/M13 R.
Figure Legend Snippet: Identification of recombinant virus rBv- APSL DNA by PCR. The PCR product was electrophoresed on 1% agarose gel. Lane 1: GeneRuler™ 1 kb DNA Ladder; Lane 2: PCR product of rBv-APSL with P1/P2; Lane 3: PCR product of rBv- APSL with P1/M13 R; Lane 4: PCR product of rBv- APSL with M13 F/P2; Lane 5: PCR product of rBv- APSL with M13 F/M13 R.

Techniques Used: Recombinant, Polymerase Chain Reaction, Agarose Gel Electrophoresis

Identification of the recombinant plasmid (pFastBac HTA- APSL ). The PCR product was electrophoresed on 1% agarose gel. ( A ) Recombinant plasmid analysis map. ( B ) Identification of the recombinant plasmid map. Lane 1: DNA Marker III (Shanghai Yuanye, China); Lane 2: PCR product of recombinant plasmid with P1/P2; Lane 3: PCR product of pFastBac HTA with P1/P2; Lane 4: double digest product ( Bam H I/ Sal I) of the recombinant plasmid.
Figure Legend Snippet: Identification of the recombinant plasmid (pFastBac HTA- APSL ). The PCR product was electrophoresed on 1% agarose gel. ( A ) Recombinant plasmid analysis map. ( B ) Identification of the recombinant plasmid map. Lane 1: DNA Marker III (Shanghai Yuanye, China); Lane 2: PCR product of recombinant plasmid with P1/P2; Lane 3: PCR product of pFastBac HTA with P1/P2; Lane 4: double digest product ( Bam H I/ Sal I) of the recombinant plasmid.

Techniques Used: Recombinant, Plasmid Preparation, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Marker

2) Product Images from "Anti-Diabetic Effects of CTB-APSL Fusion Protein in Type 2 Diabetic Mice"

Article Title: Anti-Diabetic Effects of CTB-APSL Fusion Protein in Type 2 Diabetic Mice

Journal: Marine Drugs

doi: 10.3390/md12031512

Identification of combinant virus DNA by PCR. The product was electrophoresed on 1% agarose gel. Lane M: Trans 15K DNA Marker; Lane 1: M13 F/M13 R PCR product; Lane 2: M13 F/P4 PCR product; Lane 3: P1/M13 R PCR product; Lane 4: P1/P4 PCR product.
Figure Legend Snippet: Identification of combinant virus DNA by PCR. The product was electrophoresed on 1% agarose gel. Lane M: Trans 15K DNA Marker; Lane 1: M13 F/M13 R PCR product; Lane 2: M13 F/P4 PCR product; Lane 3: P1/M13 R PCR product; Lane 4: P1/P4 PCR product.

Techniques Used: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Marker

Related Articles

Amplification:

Article Title: A Shark Liver Gene-Derived Active Peptide Expressed in the Silkworm, Bombyx mori: Preliminary Studies for Oral Administration of the Recombinant Protein
Article Snippet: Our work may pave the way for the development of an oral drug for the treatment of diabetes mellitus in the future. .. DNA manipulation and PCR amplification kits were purchased from TaKaRa Biomedicals (Kyoto, Japan). .. The Viral Genomic DNA Purification Kit was purchased from Roche Co. (San Francisco, CA, USA).

Article Title: Anti-Diabetic Effects of CTB-APSL Fusion Protein in Type 2 Diabetic Mice
Article Snippet: In conclusion, our study demonstrates for the first time that CTB-APSL fusion protein has positive effects on the control of type 2 diabetes and effectively improves its complications, thus providing an experimental basis for the development of a new type of oral therapy for type 2 diabetes. .. DNA manipulation and PCR amplification kits were purchased from TaKaRa Biomedicals (Kyoto, Japan). .. The Viral Genomic DNA Purification Kit was purchased from Roche Co. (San Francisco, CA, USA).

Article Title: Protection against Autoimmune Diabetes by Silkworm-Produced GFP-Tagged CTB-Insulin Fusion Protein
Article Snippet: This silkworm-produced active CTB-Ins-GFP protein, administered as a vaccine protein, was able to induce insulin specific oral tolerance, which is related to increased Treg cells in the treatment of T1D. .. DNA manipulation and PCR amplification kits were purchased from TaKaRa Biomedicals (Japan). .. The rabbit anticholera toxin primary serum, bacterial CTB peptides, and monosialoganglioside-GM1 were from Sigma-Aldrich (USA).

DNA Ligation:

Article Title: Identification of a Direct Biosynthetic Pathway for UDP–N-Acetylgalactosamine from Glucosamine-6-Phosphate in Thermophilic Crenarchaeon Sulfolobus tokodaii
Article Snippet: The restriction enzymes and a DNA ligation kit (Mighty Mix) were obtained from TaKaRa Bio Inc. (Shiga, Japan). .. Escherichia coli strains JM109 for DNA manipulation and BL21-CodonPlus(DE3)-RIPL and RIL for protein expression were purchased from TaKaRa Bio Inc. and Stratagene (La Jolla, CA), respectively.

Article Title: Increasing the Thermostable Sugar-1-Phosphate Nucleotidylyltransferase Activities of the Archaeal ST0452 Protein through Site Saturation Mutagenesis of the 97th Amino Acid Position
Article Snippet: The restriction enzymes NdeI, XhoI, and DpnI and a DNA ligation kit (Mighty Mix) were obtained from TaKaRa Bio Inc. (Shiga, Japan). .. E. coli strains JM109 for DNA manipulation and BL21-CodonPlus(DE3)-RIPL for protein expression were obtained, respectively, from TaKaRa Bio, Inc., and Stratagene (La Jolla, CA, USA).

Synthesized:

Article Title: Bacterial Enzymes Catalyzing the Synthesis of 1,8-Dihydroxynaphthalene, a Key Precursor of Dihydroxynaphthalene Melanin, from Sorangium cellulosum
Article Snippet: T4 HN (compound 2) was synthesized according to the method of Ichinose et al. ( ). (±)-Scytalone (compound 3) and (±)-3-hydroxy-1-tetralone (compound 11) were synthesized according to the methods of Schätzle et al. ( ). .. Escherichia coli HST08, E. coli HST04, the pColdI plasmid, restriction enzymes, and other DNA-modifying enzymes used for DNA manipulation were purchased from TaKaRa Bio Inc. (Shiga, Japan).

Article Title: High Efficient Expression, Purification, and Functional Characterization of Native Human Epidermal Growth Factor in Escherichia coli
Article Snippet: Prime STAR HS (Premix) LA Taq, DNA ladder Marker, and kits for DNA manipulation were purchased from TAKARA Biotechnology Co., Ltd. (Dalian, China). .. Protein ladder marker was obtained from Thermo Fisher Scientific (CA, USA).

TA Cloning:

Article Title: Pleiotropic role of the Sco1/SenC family copper chaperone in the physiology of Streptomyces
Article Snippet: TA cloning of polymerase chain reaction (PCR)‐generated DNA fragments was done with the help of pMD19 (Takara Shuzo). pUWLFLP carrying an Flp recombinase gene ( ) was used for the construction of a marker‐less mutant. .. The enzymes used for DNA manipulation were purchased from Takara Shuzo.

Article Title: Involvement of CarA/LitR and CRP/FNR Family Transcriptional Regulators in Light-Induced Carotenoid Production in Thermus thermophilus
Article Snippet: Escherichia coli JM109 and BL21(DE3)/pLysS (Takara-Shuzo, Kyoto, Japan) were used as hosts for DNA manipulation and protein expression, respectively. pUC19 (Takara-Shuzo) was used for general DNA manipulation. pT7Blue (Takara-Shuzo) was used for TA cloning of PCR-generated DNA fragments. pGEX-6P-2 (GE Healthcare Bio-Sciences KK, Tokyo, Japan) was used for the expression of LitR and TT_P0055 (here designated TTP55) protein in E. coli. .. Enzymes used for DNA manipulation were purchased from Takara-Shuzo.

Article Title: Role and Function of LitR, an Adenosyl B12-Bound Light-Sensitive Regulator of Bacillus megaterium QM B1551, in Regulation of Carotenoid Production
Article Snippet: Escherichia coli HST08 and Rosetta2(DE3)pLysS (TaKaRa Bio Inc., Shiga, Japan) were used as hosts for DNA manipulation and protein expression, respectively. pUC19 and pUC118 (TaKaRa Bio) were used for general DNA manipulation in E. coli . pT7Blue and pMD19 (TaKaRa Bio) were used for TA cloning of PCR-generated DNA fragments. pGEX-6P-2 (GE Healthcare UK Ltd., Buckinghamshire, England) and pET51-b(+) (TaKaRa Bio) were used for the overexpression of B. megaterium QM B1551 LitR and SigA, respectively. pUCTV2, an E. coli - Bacillus temperature-sensitive plasmid shuttle vector ( ) carrying a tetracycline resistance gene, was obtained from F. Meinhardt and used for gene disruption and complementation analysis of B. megaterium . pDG1661 (carrying a chloramphenicol resistance gene and amyE for homologous recombination) was obtained from the BGSC and used as a chromosomal integration plasmid for B. subtilis . .. Enzymes used for DNA manipulation were purchased from TaKaRa Bio and New England BioLabs (Ipswich, MA).

Construct:

Article Title: Anti-Diabetic Effects of CTB-APSL Fusion Protein in Type 2 Diabetic Mice
Article Snippet: DNA manipulation and PCR amplification kits were purchased from TaKaRa Biomedicals (Kyoto, Japan). .. The Bombyx mori N cells (BmN cells), which originated from the ovary, were maintained in our laboratory and cultured at 27 °C in Sf-900 II Serum Free Medium Complete (GIBCO, Gran Island, NY, USA) containing 10% fetal bovine serum (GIBCO, Gran Island, NY, USA).

HAT Assay:

Article Title: Genetic and Transcriptional Analyses of the Flagellar Gene Cluster in Actinoplanes missouriensis
Article Snippet: The trace element solution contained 0.004% ZnCl2 , 0.02% FeCl3 ·6H2 O, 0.001% CuCl2 ·2H2 O, 0.001% MnCl2 ·4H2 O, 0.001% Na2 B4 O7 ·10H2 O, and 0.001% (NH4 )6 Mo7 O24 ·4H2 O. HAT agar was used for sporangium formation. .. E. coli JM109 and pUC19 for DNA manipulation were purchased from TaKaRa Biochemicals (Shiga, Japan). pGEM-T Easy was purchased from Promega (Tokyo, Japan). pK19mobsacB was obtained from NITE. pTYM19 was obtained from H. Onaka ( ).

Expressing:

Article Title: Involvement of CarA/LitR and CRP/FNR Family Transcriptional Regulators in Light-Induced Carotenoid Production in Thermus thermophilus
Article Snippet: Escherichia coli JM109 and BL21(DE3)/pLysS (Takara-Shuzo, Kyoto, Japan) were used as hosts for DNA manipulation and protein expression, respectively. pUC19 (Takara-Shuzo) was used for general DNA manipulation. pT7Blue (Takara-Shuzo) was used for TA cloning of PCR-generated DNA fragments. pGEX-6P-2 (GE Healthcare Bio-Sciences KK, Tokyo, Japan) was used for the expression of LitR and TT_P0055 (here designated TTP55) protein in E. coli. .. Enzymes used for DNA manipulation were purchased from Takara-Shuzo.

Article Title: Role and Function of LitR, an Adenosyl B12-Bound Light-Sensitive Regulator of Bacillus megaterium QM B1551, in Regulation of Carotenoid Production
Article Snippet: Escherichia coli HST08 and Rosetta2(DE3)pLysS (TaKaRa Bio Inc., Shiga, Japan) were used as hosts for DNA manipulation and protein expression, respectively. pUC19 and pUC118 (TaKaRa Bio) were used for general DNA manipulation in E. coli . pT7Blue and pMD19 (TaKaRa Bio) were used for TA cloning of PCR-generated DNA fragments. pGEX-6P-2 (GE Healthcare UK Ltd., Buckinghamshire, England) and pET51-b(+) (TaKaRa Bio) were used for the overexpression of B. megaterium QM B1551 LitR and SigA, respectively. pUCTV2, an E. coli - Bacillus temperature-sensitive plasmid shuttle vector ( ) carrying a tetracycline resistance gene, was obtained from F. Meinhardt and used for gene disruption and complementation analysis of B. megaterium . pDG1661 (carrying a chloramphenicol resistance gene and amyE for homologous recombination) was obtained from the BGSC and used as a chromosomal integration plasmid for B. subtilis . .. Enzymes used for DNA manipulation were purchased from TaKaRa Bio and New England BioLabs (Ipswich, MA).

Article Title: Biosynthesis of Aliphatic Polyketides by Type III Polyketide Synthase and Methyltransferase in Bacillus subtilis
Article Snippet: Escherichia coli JM109 and BL21(DE3), plasmids pUC19 and pColdI, restriction enzymes, and other DNA-modifying enzymes used for DNA manipulation were purchased from Takara Biochemicals (Shiga, Japan). .. Escherichia coli JM109 and BL21(DE3), plasmids pUC19 and pColdI, restriction enzymes, and other DNA-modifying enzymes used for DNA manipulation were purchased from Takara Biochemicals (Shiga, Japan).

Article Title: Identification of a Direct Biosynthetic Pathway for UDP–N-Acetylgalactosamine from Glucosamine-6-Phosphate in Thermophilic Crenarchaeon Sulfolobus tokodaii
Article Snippet: Growth of S. tokodaii and extraction of its genomic DNA were carried out according to a previously described protocol ( ). .. Escherichia coli strains JM109 for DNA manipulation and BL21-CodonPlus(DE3)-RIPL and RIL for protein expression were purchased from TaKaRa Bio Inc. and Stratagene (La Jolla, CA), respectively. .. The expression plasmid vectors pET21a, pET23b, and pET28a were purchased from Novagen (Madison, WI).

Article Title: Increasing the Thermostable Sugar-1-Phosphate Nucleotidylyltransferase Activities of the Archaeal ST0452 Protein through Site Saturation Mutagenesis of the 97th Amino Acid Position
Article Snippet: KOD-plus-Neo DNA polymerase was purchased from Toyobo Co., Ltd. (Osaka, Japan). .. E. coli strains JM109 for DNA manipulation and BL21-CodonPlus(DE3)-RIPL for protein expression were obtained, respectively, from TaKaRa Bio, Inc., and Stratagene (La Jolla, CA, USA). .. The plasmid vector pET-21a(+) was purchased from Novagen (Madison, WI, USA).

Transformation Assay:

Article Title: Two Glycine Riboswitches Activate the Glycine Cleavage System Essential for Glycine Detoxification in Streptomyces griseus
Article Snippet: Escherichia coli JM109 and the pUC19 vector for DNA manipulation were purchased from TaKaRa Biochemicals. .. Escherichia coli JM109 and the pUC19 vector for DNA manipulation were purchased from TaKaRa Biochemicals.

Article Title: Genetic and Transcriptional Analyses of the Flagellar Gene Cluster in Actinoplanes missouriensis
Article Snippet: GYMC medium (0.4% glucose, 0.4% yeast extract, 1% malt extract [Difco], 0.2% CaCO3 [pH 7.2]) was used for transformation by conjugation with Escherichia coli ET12567(pUZ8002). .. E. coli JM109 and pUC19 for DNA manipulation were purchased from TaKaRa Biochemicals (Shiga, Japan). pGEM-T Easy was purchased from Promega (Tokyo, Japan). pK19mobsacB was obtained from NITE. pTYM19 was obtained from H. Onaka ( ).

Over Expression:

Article Title: Role and Function of LitR, an Adenosyl B12-Bound Light-Sensitive Regulator of Bacillus megaterium QM B1551, in Regulation of Carotenoid Production
Article Snippet: Escherichia coli HST08 and Rosetta2(DE3)pLysS (TaKaRa Bio Inc., Shiga, Japan) were used as hosts for DNA manipulation and protein expression, respectively. pUC19 and pUC118 (TaKaRa Bio) were used for general DNA manipulation in E. coli . pT7Blue and pMD19 (TaKaRa Bio) were used for TA cloning of PCR-generated DNA fragments. pGEX-6P-2 (GE Healthcare UK Ltd., Buckinghamshire, England) and pET51-b(+) (TaKaRa Bio) were used for the overexpression of B. megaterium QM B1551 LitR and SigA, respectively. pUCTV2, an E. coli - Bacillus temperature-sensitive plasmid shuttle vector ( ) carrying a tetracycline resistance gene, was obtained from F. Meinhardt and used for gene disruption and complementation analysis of B. megaterium . pDG1661 (carrying a chloramphenicol resistance gene and amyE for homologous recombination) was obtained from the BGSC and used as a chromosomal integration plasmid for B. subtilis . .. Enzymes used for DNA manipulation were purchased from TaKaRa Bio and New England BioLabs (Ipswich, MA).

Conjugation Assay:

Article Title: Genetic and Transcriptional Analyses of the Flagellar Gene Cluster in Actinoplanes missouriensis
Article Snippet: GYMC medium (0.4% glucose, 0.4% yeast extract, 1% malt extract [Difco], 0.2% CaCO3 [pH 7.2]) was used for transformation by conjugation with Escherichia coli ET12567(pUZ8002). .. E. coli JM109 and pUC19 for DNA manipulation were purchased from TaKaRa Biochemicals (Shiga, Japan). pGEM-T Easy was purchased from Promega (Tokyo, Japan). pK19mobsacB was obtained from NITE. pTYM19 was obtained from H. Onaka ( ).

Gas Chromatography:

Article Title: Increasing the Thermostable Sugar-1-Phosphate Nucleotidylyltransferase Activities of the Archaeal ST0452 Protein through Site Saturation Mutagenesis of the 97th Amino Acid Position
Article Snippet: UTP, GlcNAc-1-P, Glc-1-P, UDP-GlcNAc, and UDP-Glc were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). .. E. coli strains JM109 for DNA manipulation and BL21-CodonPlus(DE3)-RIPL for protein expression were obtained, respectively, from TaKaRa Bio, Inc., and Stratagene (La Jolla, CA, USA).

Chromatography:

Article Title: A Chimeric Protein That Functions as both an Anthrax Dual-Target Antitoxin and a Trivalent Vaccine
Article Snippet: The enzymes used for DNA manipulation were purchased from TaKaRa Biotechnology Co., Ltd. .. Oligonucleotide synthesis and DNA sequencing assays were conducted at Shanghai Sangon Biological Engineering Technology & Services Co., Ltd. Rabbit anti-PA and anti-LF polyclonal antibodies (Abs) were prepared in our laboratory, and horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG, IgG1, and IgG2a were purchased from SouthernBiotech (Birmingham, AL).

Cell Culture:

Article Title: A Shark Liver Gene-Derived Active Peptide Expressed in the Silkworm, Bombyx mori: Preliminary Studies for Oral Administration of the Recombinant Protein
Article Snippet: DNA manipulation and PCR amplification kits were purchased from TaKaRa Biomedicals (Kyoto, Japan). .. DNA manipulation and PCR amplification kits were purchased from TaKaRa Biomedicals (Kyoto, Japan).

Article Title: Anti-Diabetic Effects of CTB-APSL Fusion Protein in Type 2 Diabetic Mice
Article Snippet: DNA manipulation and PCR amplification kits were purchased from TaKaRa Biomedicals (Kyoto, Japan). .. The pFasBac1 plasmid and the Lipofectamine 2000 Reagent were purchased from Invitrogen (Carlsbad, CA, USA).

Article Title: Pleiotropic role of the Sco1/SenC family copper chaperone in the physiology of Streptomyces
Article Snippet: The enzymes used for DNA manipulation were purchased from Takara Shuzo. .. The enzymes used for DNA manipulation were purchased from Takara Shuzo.

Article Title: Protection against Autoimmune Diabetes by Silkworm-Produced GFP-Tagged CTB-Insulin Fusion Protein
Article Snippet: DNA manipulation and PCR amplification kits were purchased from TaKaRa Biomedicals (Japan). .. DNA manipulation and PCR amplification kits were purchased from TaKaRa Biomedicals (Japan).

Article Title: Biosynthesis of Aliphatic Polyketides by Type III Polyketide Synthase and Methyltransferase in Bacillus subtilis
Article Snippet: Escherichia coli JM109 and BL21(DE3), plasmids pUC19 and pColdI, restriction enzymes, and other DNA-modifying enzymes used for DNA manipulation were purchased from Takara Biochemicals (Shiga, Japan). .. For the expression of bpsA and bpsB in B. subtilis , pWH1530 (MoBiTec, Florida) was used.

DNA Sequencing:

Article Title: A Chimeric Protein That Functions as both an Anthrax Dual-Target Antitoxin and a Trivalent Vaccine
Article Snippet: The enzymes used for DNA manipulation were purchased from TaKaRa Biotechnology Co., Ltd. .. The DNA extraction kits were purchased from Axygen (Hangzhou, China).

Sequencing:

Article Title: An OmpA Family Protein, a Target of the GinI/GinR Quorum-Sensing System in Gluconacetobacter intermedius, Controls Acetic Acid Fermentation
Article Snippet: A ginI -disrupted mutant ( ginI ::Km), a ginR -disrupted mutant ( ginR ::Km), and a ginA -disrupted mutant ( ginA ::Km) were described previously ( ). pGinR, pGinI, pGinIA, and pGinA, all of which were pMV24-derived plasmids, contained the intact ginR , ginI , ginI and ginA , and ginA sequences, as described previously ( ). pMGinA contained a ginA sequence with a frameshift mutation ( ). .. E. coli JM109 and plasmid pUC19, used for DNA manipulation, were purchased from Takara Bio.

Recombinant:

Article Title: Bacterial Enzymes Catalyzing the Synthesis of 1,8-Dihydroxynaphthalene, a Key Precursor of Dihydroxynaphthalene Melanin, from Sorangium cellulosum
Article Snippet: Flaviolin (compound 6) was prepared from a recombinant S. coelicolor M1146 strain harboring pNF2 ( ). .. Escherichia coli HST08, E. coli HST04, the pColdI plasmid, restriction enzymes, and other DNA-modifying enzymes used for DNA manipulation were purchased from TaKaRa Bio Inc. (Shiga, Japan).

Article Title: High Efficient Expression, Purification, and Functional Characterization of Native Human Epidermal Growth Factor in Escherichia coli
Article Snippet: Prime STAR HS (Premix) LA Taq, DNA ladder Marker, and kits for DNA manipulation were purchased from TAKARA Biotechnology Co., Ltd. (Dalian, China). .. Pfu DNA polymerase was purchased from Promega (Madison, USA). hEGF coding region was synthesized by Huada Genomics Institute Co., Ltd. (Shenzhen, China).

Molecular Weight:

Article Title: Increasing the Thermostable Sugar-1-Phosphate Nucleotidylyltransferase Activities of the Archaeal ST0452 Protein through Site Saturation Mutagenesis of the 97th Amino Acid Position
Article Snippet: E. coli strains JM109 for DNA manipulation and BL21-CodonPlus(DE3)-RIPL for protein expression were obtained, respectively, from TaKaRa Bio, Inc., and Stratagene (La Jolla, CA, USA). .. The plasmid vector pET-21a(+) was purchased from Novagen (Madison, WI, USA).

Methylation:

Article Title: Pleiotropic role of the Sco1/SenC family copper chaperone in the physiology of Streptomyces
Article Snippet: The Escherichia coli DH5α strain (Takara Shuzo; Kyoto, Japan) was used as a host for conventional DNA manipulation, and the GM2163 strain, a methylation‐deficient strain, was used for generating disruption cosmids. pUC19 (Takara Shuzo) was used for general DNA manipulation. .. The enzymes used for DNA manipulation were purchased from Takara Shuzo.

Mutagenesis:

Article Title: Pleiotropic role of the Sco1/SenC family copper chaperone in the physiology of Streptomyces
Article Snippet: TA cloning of polymerase chain reaction (PCR)‐generated DNA fragments was done with the help of pMD19 (Takara Shuzo). pUWLFLP carrying an Flp recombinase gene ( ) was used for the construction of a marker‐less mutant. .. The enzymes used for DNA manipulation were purchased from Takara Shuzo.

Article Title: An OmpA Family Protein, a Target of the GinI/GinR Quorum-Sensing System in Gluconacetobacter intermedius, Controls Acetic Acid Fermentation
Article Snippet: A ginI -disrupted mutant ( ginI ::Km), a ginR -disrupted mutant ( ginR ::Km), and a ginA -disrupted mutant ( ginA ::Km) were described previously ( ). pGinR, pGinI, pGinIA, and pGinA, all of which were pMV24-derived plasmids, contained the intact ginR , ginI , ginI and ginA , and ginA sequences, as described previously ( ). pMGinA contained a ginA sequence with a frameshift mutation ( ). .. E. coli JM109 and plasmid pUC19, used for DNA manipulation, were purchased from Takara Bio.

Article Title: Two ATP-Binding Cassette Transporters Involved in (S)-2-Aminoethyl-Cysteine Uptake in Thermus thermophilus
Article Snippet: The 2× YT medium ( ) generally was used for cultivation of E. coli cells, whereas TM (nutrient medium) ( ) and MM (minimal medium) ( ) were used for cultivation of T. thermophilus HB27 and mutant strains. .. Enzymes for DNA manipulation were purchased from TaKaRa Shuzo (Kyoto, Japan) and Toyobo (Osaka, Japan).

Mouse Assay:

Article Title: Protection against Autoimmune Diabetes by Silkworm-Produced GFP-Tagged CTB-Insulin Fusion Protein
Article Snippet: Paragraph title: 2.1. Reagents, Cell Lines, Silkworms, and Mice ... DNA manipulation and PCR amplification kits were purchased from TaKaRa Biomedicals (Japan).

Polymerase Chain Reaction:

Article Title: A Shark Liver Gene-Derived Active Peptide Expressed in the Silkworm, Bombyx mori: Preliminary Studies for Oral Administration of the Recombinant Protein
Article Snippet: Our work may pave the way for the development of an oral drug for the treatment of diabetes mellitus in the future. .. DNA manipulation and PCR amplification kits were purchased from TaKaRa Biomedicals (Kyoto, Japan). .. The Viral Genomic DNA Purification Kit was purchased from Roche Co. (San Francisco, CA, USA).

Article Title: Anti-Diabetic Effects of CTB-APSL Fusion Protein in Type 2 Diabetic Mice
Article Snippet: In conclusion, our study demonstrates for the first time that CTB-APSL fusion protein has positive effects on the control of type 2 diabetes and effectively improves its complications, thus providing an experimental basis for the development of a new type of oral therapy for type 2 diabetes. .. DNA manipulation and PCR amplification kits were purchased from TaKaRa Biomedicals (Kyoto, Japan). .. The Viral Genomic DNA Purification Kit was purchased from Roche Co. (San Francisco, CA, USA).

Article Title: Pleiotropic role of the Sco1/SenC family copper chaperone in the physiology of Streptomyces
Article Snippet: TA cloning of polymerase chain reaction (PCR)‐generated DNA fragments was done with the help of pMD19 (Takara Shuzo). pUWLFLP carrying an Flp recombinase gene ( ) was used for the construction of a marker‐less mutant. .. The enzymes used for DNA manipulation were purchased from Takara Shuzo.

Article Title: Protection against Autoimmune Diabetes by Silkworm-Produced GFP-Tagged CTB-Insulin Fusion Protein
Article Snippet: This silkworm-produced active CTB-Ins-GFP protein, administered as a vaccine protein, was able to induce insulin specific oral tolerance, which is related to increased Treg cells in the treatment of T1D. .. DNA manipulation and PCR amplification kits were purchased from TaKaRa Biomedicals (Japan). .. The rabbit anticholera toxin primary serum, bacterial CTB peptides, and monosialoganglioside-GM1 were from Sigma-Aldrich (USA).

Article Title: Involvement of CarA/LitR and CRP/FNR Family Transcriptional Regulators in Light-Induced Carotenoid Production in Thermus thermophilus
Article Snippet: Escherichia coli JM109 and BL21(DE3)/pLysS (Takara-Shuzo, Kyoto, Japan) were used as hosts for DNA manipulation and protein expression, respectively. pUC19 (Takara-Shuzo) was used for general DNA manipulation. pT7Blue (Takara-Shuzo) was used for TA cloning of PCR-generated DNA fragments. pGEX-6P-2 (GE Healthcare Bio-Sciences KK, Tokyo, Japan) was used for the expression of LitR and TT_P0055 (here designated TTP55) protein in E. coli. .. Enzymes used for DNA manipulation were purchased from Takara-Shuzo.

Article Title: Role and Function of LitR, an Adenosyl B12-Bound Light-Sensitive Regulator of Bacillus megaterium QM B1551, in Regulation of Carotenoid Production
Article Snippet: Escherichia coli HST08 and Rosetta2(DE3)pLysS (TaKaRa Bio Inc., Shiga, Japan) were used as hosts for DNA manipulation and protein expression, respectively. pUC19 and pUC118 (TaKaRa Bio) were used for general DNA manipulation in E. coli . pT7Blue and pMD19 (TaKaRa Bio) were used for TA cloning of PCR-generated DNA fragments. pGEX-6P-2 (GE Healthcare UK Ltd., Buckinghamshire, England) and pET51-b(+) (TaKaRa Bio) were used for the overexpression of B. megaterium QM B1551 LitR and SigA, respectively. pUCTV2, an E. coli - Bacillus temperature-sensitive plasmid shuttle vector ( ) carrying a tetracycline resistance gene, was obtained from F. Meinhardt and used for gene disruption and complementation analysis of B. megaterium . pDG1661 (carrying a chloramphenicol resistance gene and amyE for homologous recombination) was obtained from the BGSC and used as a chromosomal integration plasmid for B. subtilis . .. Enzymes used for DNA manipulation were purchased from TaKaRa Bio and New England BioLabs (Ipswich, MA).

Positron Emission Tomography:

Article Title: Identification and Gene Disruption of Small Noncoding RNAs in Streptomyces griseus
Article Snippet: Escherichia coli JM109 and the vector pUC19 for DNA manipulation were purchased from Takara Biochemicals. .. Escherichia coli JM109 and the vector pUC19 for DNA manipulation were purchased from Takara Biochemicals.

Purification:

Article Title: Identification and Gene Disruption of Small Noncoding RNAs in Streptomyces griseus
Article Snippet: Escherichia coli JM109 and the vector pUC19 for DNA manipulation were purchased from Takara Biochemicals. .. Escherichia coli JM109 and the vector pUC19 for DNA manipulation were purchased from Takara Biochemicals.

Plasmid Preparation:

Article Title: A Shark Liver Gene-Derived Active Peptide Expressed in the Silkworm, Bombyx mori: Preliminary Studies for Oral Administration of the Recombinant Protein
Article Snippet: DNA manipulation and PCR amplification kits were purchased from TaKaRa Biomedicals (Kyoto, Japan). .. The Viral Genomic DNA Purification Kit was purchased from Roche Co. (San Francisco, CA, USA).

Article Title: Anti-Diabetic Effects of CTB-APSL Fusion Protein in Type 2 Diabetic Mice
Article Snippet: DNA manipulation and PCR amplification kits were purchased from TaKaRa Biomedicals (Kyoto, Japan). .. The Viral Genomic DNA Purification Kit was purchased from Roche Co. (San Francisco, CA, USA).

Article Title: Bacterial Enzymes Catalyzing the Synthesis of 1,8-Dihydroxynaphthalene, a Key Precursor of Dihydroxynaphthalene Melanin, from Sorangium cellulosum
Article Snippet: S. cellulosum So ce56 was purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ, Braunschweig, Germany). .. Escherichia coli HST08, E. coli HST04, the pColdI plasmid, restriction enzymes, and other DNA-modifying enzymes used for DNA manipulation were purchased from TaKaRa Bio Inc. (Shiga, Japan). .. E. coli BL21 was purchased from the National BioResource Project (National Institute of Genetics of Japan).

Article Title: Identification and Gene Disruption of Small Noncoding RNAs in Streptomyces griseus
Article Snippet: Neomycin (20 μg/ml) was added when necessary. .. Escherichia coli JM109 and the vector pUC19 for DNA manipulation were purchased from Takara Biochemicals. .. E. coli JM110 containing dam and dcm mutations was used for preparing nonmethylated Streptomyces DNA for gene disruption.

Article Title: Two Glycine Riboswitches Activate the Glycine Cleavage System Essential for Glycine Detoxification in Streptomyces griseus
Article Snippet: Neomycin (20 μg/ml) was added when necessary. .. Escherichia coli JM109 and the pUC19 vector for DNA manipulation were purchased from TaKaRa Biochemicals. .. E. coli IR539 was obtained from Riken ( ).

Article Title: The O-Methyltransferase SrsB Catalyzes the Decarboxylative Methylation of Alkylresorcylic Acid during Phenolic Lipid Biosynthesis by Streptomyces griseus
Article Snippet: However, detailed in vitro analyses are required to confirm the proposed enzymatic reactions, especially those reactions catalyzed by SrsA and SrsB, for the reasons given below. .. Escherichia coli strain JM109, plasmid pUC19, restriction enzymes, and other DNA-modifying enzymes used for DNA manipulation were purchased from Takara Bio (Otsu, Japan). .. S. griseus IFO13350 was obtained from the Institute of Fermentation, Osaka, Japan (IFO).

Article Title: Involvement of CarA/LitR and CRP/FNR Family Transcriptional Regulators in Light-Induced Carotenoid Production in Thermus thermophilus
Article Snippet: Enzymes used for DNA manipulation were purchased from Takara-Shuzo. .. Enzymes used for DNA manipulation were purchased from Takara-Shuzo.

Article Title: An OmpA Family Protein, a Target of the GinI/GinR Quorum-Sensing System in Gluconacetobacter intermedius, Controls Acetic Acid Fermentation
Article Snippet: A ginI -disrupted mutant ( ginI ::Km), a ginR -disrupted mutant ( ginR ::Km), and a ginA -disrupted mutant ( ginA ::Km) were described previously ( ). pGinR, pGinI, pGinIA, and pGinA, all of which were pMV24-derived plasmids, contained the intact ginR , ginI , ginI and ginA , and ginA sequences, as described previously ( ). pMGinA contained a ginA sequence with a frameshift mutation ( ). .. E. coli JM109 and plasmid pUC19, used for DNA manipulation, were purchased from Takara Bio. .. Agrobacterium tumefaciens NTL4(pZLR4), obtained from S. K. Farrand, was used to assay AHL production.

Article Title: Role and Function of LitR, an Adenosyl B12-Bound Light-Sensitive Regulator of Bacillus megaterium QM B1551, in Regulation of Carotenoid Production
Article Snippet: Escherichia coli HST08 and Rosetta2(DE3)pLysS (TaKaRa Bio Inc., Shiga, Japan) were used as hosts for DNA manipulation and protein expression, respectively. pUC19 and pUC118 (TaKaRa Bio) were used for general DNA manipulation in E. coli . pT7Blue and pMD19 (TaKaRa Bio) were used for TA cloning of PCR-generated DNA fragments. pGEX-6P-2 (GE Healthcare UK Ltd., Buckinghamshire, England) and pET51-b(+) (TaKaRa Bio) were used for the overexpression of B. megaterium QM B1551 LitR and SigA, respectively. pUCTV2, an E. coli - Bacillus temperature-sensitive plasmid shuttle vector ( ) carrying a tetracycline resistance gene, was obtained from F. Meinhardt and used for gene disruption and complementation analysis of B. megaterium . pDG1661 (carrying a chloramphenicol resistance gene and amyE for homologous recombination) was obtained from the BGSC and used as a chromosomal integration plasmid for B. subtilis . .. Enzymes used for DNA manipulation were purchased from TaKaRa Bio and New England BioLabs (Ipswich, MA).

Selection:

Article Title: Role and Function of LitR, an Adenosyl B12-Bound Light-Sensitive Regulator of Bacillus megaterium QM B1551, in Regulation of Carotenoid Production
Article Snippet: Enzymes used for DNA manipulation were purchased from TaKaRa Bio and New England BioLabs (Ipswich, MA). .. Enzymes used for DNA manipulation were purchased from TaKaRa Bio and New England BioLabs (Ipswich, MA).

Oligonucleotide Synthesis:

Article Title: A Chimeric Protein That Functions as both an Anthrax Dual-Target Antitoxin and a Trivalent Vaccine
Article Snippet: The enzymes used for DNA manipulation were purchased from TaKaRa Biotechnology Co., Ltd. .. The DNA extraction kits were purchased from Axygen (Hangzhou, China).

Marker:

Article Title: High Efficient Expression, Purification, and Functional Characterization of Native Human Epidermal Growth Factor in Escherichia coli
Article Snippet: Restriction endonucleases Nde I and Xho I were purchased from Thermo Fisher Scientific Co., Ltd. (Shanghai, China). .. Prime STAR HS (Premix) LA Taq, DNA ladder Marker, and kits for DNA manipulation were purchased from TAKARA Biotechnology Co., Ltd. (Dalian, China). .. Protein ladder marker was obtained from Thermo Fisher Scientific (CA, USA).

Article Title: Pleiotropic role of the Sco1/SenC family copper chaperone in the physiology of Streptomyces
Article Snippet: TA cloning of polymerase chain reaction (PCR)‐generated DNA fragments was done with the help of pMD19 (Takara Shuzo). pUWLFLP carrying an Flp recombinase gene ( ) was used for the construction of a marker‐less mutant. .. The enzymes used for DNA manipulation were purchased from Takara Shuzo.

Staining:

Article Title: Protection against Autoimmune Diabetes by Silkworm-Produced GFP-Tagged CTB-Insulin Fusion Protein
Article Snippet: DNA manipulation and PCR amplification kits were purchased from TaKaRa Biomedicals (Japan). .. The rabbit anticholera toxin primary serum, bacterial CTB peptides, and monosialoganglioside-GM1 were from Sigma-Aldrich (USA).

Homologous Recombination:

Article Title: Role and Function of LitR, an Adenosyl B12-Bound Light-Sensitive Regulator of Bacillus megaterium QM B1551, in Regulation of Carotenoid Production
Article Snippet: Escherichia coli HST08 and Rosetta2(DE3)pLysS (TaKaRa Bio Inc., Shiga, Japan) were used as hosts for DNA manipulation and protein expression, respectively. pUC19 and pUC118 (TaKaRa Bio) were used for general DNA manipulation in E. coli . pT7Blue and pMD19 (TaKaRa Bio) were used for TA cloning of PCR-generated DNA fragments. pGEX-6P-2 (GE Healthcare UK Ltd., Buckinghamshire, England) and pET51-b(+) (TaKaRa Bio) were used for the overexpression of B. megaterium QM B1551 LitR and SigA, respectively. pUCTV2, an E. coli - Bacillus temperature-sensitive plasmid shuttle vector ( ) carrying a tetracycline resistance gene, was obtained from F. Meinhardt and used for gene disruption and complementation analysis of B. megaterium . pDG1661 (carrying a chloramphenicol resistance gene and amyE for homologous recombination) was obtained from the BGSC and used as a chromosomal integration plasmid for B. subtilis . .. Enzymes used for DNA manipulation were purchased from TaKaRa Bio and New England BioLabs (Ipswich, MA).

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    TaKaRa dna manipulation
    Identification of rBacmid- APSL by <t>PCR.</t> The PCR product was electrophoresed on 1% agarose gel. Lane 1: 250 bp <t>DNA</t> ladder marker; Lane 2: PCR product of rBacmid- APSL with M13 F/M13 R; Lane 3: negative control (PCR product of wild-type bacmid with M13 F/M13 R).
    Dna Manipulation, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Identification of rBacmid- APSL by PCR. The PCR product was electrophoresed on 1% agarose gel. Lane 1: 250 bp DNA ladder marker; Lane 2: PCR product of rBacmid- APSL with M13 F/M13 R; Lane 3: negative control (PCR product of wild-type bacmid with M13 F/M13 R).

    Journal: Marine Drugs

    Article Title: A Shark Liver Gene-Derived Active Peptide Expressed in the Silkworm, Bombyx mori: Preliminary Studies for Oral Administration of the Recombinant Protein

    doi: 10.3390/md11051492

    Figure Lengend Snippet: Identification of rBacmid- APSL by PCR. The PCR product was electrophoresed on 1% agarose gel. Lane 1: 250 bp DNA ladder marker; Lane 2: PCR product of rBacmid- APSL with M13 F/M13 R; Lane 3: negative control (PCR product of wild-type bacmid with M13 F/M13 R).

    Article Snippet: DNA manipulation and PCR amplification kits were purchased from TaKaRa Biomedicals (Kyoto, Japan).

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Marker, Negative Control

    Identification of recombinant virus rBv- APSL DNA by PCR. The PCR product was electrophoresed on 1% agarose gel. Lane 1: GeneRuler™ 1 kb DNA Ladder; Lane 2: PCR product of rBv-APSL with P1/P2; Lane 3: PCR product of rBv- APSL with P1/M13 R; Lane 4: PCR product of rBv- APSL with M13 F/P2; Lane 5: PCR product of rBv- APSL with M13 F/M13 R.

    Journal: Marine Drugs

    Article Title: A Shark Liver Gene-Derived Active Peptide Expressed in the Silkworm, Bombyx mori: Preliminary Studies for Oral Administration of the Recombinant Protein

    doi: 10.3390/md11051492

    Figure Lengend Snippet: Identification of recombinant virus rBv- APSL DNA by PCR. The PCR product was electrophoresed on 1% agarose gel. Lane 1: GeneRuler™ 1 kb DNA Ladder; Lane 2: PCR product of rBv-APSL with P1/P2; Lane 3: PCR product of rBv- APSL with P1/M13 R; Lane 4: PCR product of rBv- APSL with M13 F/P2; Lane 5: PCR product of rBv- APSL with M13 F/M13 R.

    Article Snippet: DNA manipulation and PCR amplification kits were purchased from TaKaRa Biomedicals (Kyoto, Japan).

    Techniques: Recombinant, Polymerase Chain Reaction, Agarose Gel Electrophoresis

    Identification of the recombinant plasmid (pFastBac HTA- APSL ). The PCR product was electrophoresed on 1% agarose gel. ( A ) Recombinant plasmid analysis map. ( B ) Identification of the recombinant plasmid map. Lane 1: DNA Marker III (Shanghai Yuanye, China); Lane 2: PCR product of recombinant plasmid with P1/P2; Lane 3: PCR product of pFastBac HTA with P1/P2; Lane 4: double digest product ( Bam H I/ Sal I) of the recombinant plasmid.

    Journal: Marine Drugs

    Article Title: A Shark Liver Gene-Derived Active Peptide Expressed in the Silkworm, Bombyx mori: Preliminary Studies for Oral Administration of the Recombinant Protein

    doi: 10.3390/md11051492

    Figure Lengend Snippet: Identification of the recombinant plasmid (pFastBac HTA- APSL ). The PCR product was electrophoresed on 1% agarose gel. ( A ) Recombinant plasmid analysis map. ( B ) Identification of the recombinant plasmid map. Lane 1: DNA Marker III (Shanghai Yuanye, China); Lane 2: PCR product of recombinant plasmid with P1/P2; Lane 3: PCR product of pFastBac HTA with P1/P2; Lane 4: double digest product ( Bam H I/ Sal I) of the recombinant plasmid.

    Article Snippet: DNA manipulation and PCR amplification kits were purchased from TaKaRa Biomedicals (Kyoto, Japan).

    Techniques: Recombinant, Plasmid Preparation, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Marker

    Identification of combinant virus DNA by PCR. The product was electrophoresed on 1% agarose gel. Lane M: Trans 15K DNA Marker; Lane 1: M13 F/M13 R PCR product; Lane 2: M13 F/P4 PCR product; Lane 3: P1/M13 R PCR product; Lane 4: P1/P4 PCR product.

    Journal: Marine Drugs

    Article Title: Anti-Diabetic Effects of CTB-APSL Fusion Protein in Type 2 Diabetic Mice

    doi: 10.3390/md12031512

    Figure Lengend Snippet: Identification of combinant virus DNA by PCR. The product was electrophoresed on 1% agarose gel. Lane M: Trans 15K DNA Marker; Lane 1: M13 F/M13 R PCR product; Lane 2: M13 F/P4 PCR product; Lane 3: P1/M13 R PCR product; Lane 4: P1/P4 PCR product.

    Article Snippet: DNA manipulation and PCR amplification kits were purchased from TaKaRa Biomedicals (Kyoto, Japan).

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Marker