rnalater stabilization solution  (Thermo Fisher)


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    Name:
    RNAlater Stabilization Solution
    Description:
    RNAlater RNA Stabilization Solution stabilizes and protects cellular RNA in intact unfrozen tissue samples eliminating the need to immediately process tissue samples or to freeze samples in liquid nitrogen for later processing Tissue pieces can be harvested and submerged in RNAlater RNA Stabilization Solution for storage without jeopardizing the quality or quantity of RNA obtained after subsequent RNA isolation Advantages of using RNAlater RNA Stabilization Solution • Effectiveness stabilize RNA for 1 day at 37°C 1 week at 25°C 1 month at 4°C or indefinitely at 20°C• Simplicity a single reagent that immediately inactivates RNases and stabilizes RNA within tissues or cells• Convenience no need to freeze samples in liquid nitrogen or rush samples back to the lab freezer• Mobility perfect for tissue collection in the field • Versatility compatible with many RNA isolation procedures including most RNA isolation kitsApplicationsRNAlater RNA Stabilization Solution has been tested on a variety of mammalian tissues plants E coli Xenopus fish and Drosophila It is ideal for • Protecting RNA integrity in tissues rich in RNases• Collecting samples from different time points without having to process the samples from each time point immediately• Archiving tissues for future microdissection• Submerging animal cavities or organs to stabilize RNA during lengthy dissection procedures• Collecting samples at locations e g hospitals field sites the space shuttle where immediate RNA isolation is not possible• Shipping samples on wet ice or even at room temperature if shipped overnightRNAlater RNA Stabilization Solution procedureThe dissected tissue less than 0 5 cm in any one dimension is simply submerged in approximately 5 volumes of RNAlater solution at room temperature The solution permeates the cells stabilizing the RNA The sample can then be stored indefinitely at 20°C the tissue does not freeze at 4°C for up to a month or at 25°C for up to a week For RNA isolation the tissue is simply removed from RNAlater solution and treated as though it had just been harvested Most tissues can be transferred directly to a lysis buffer and homogenized Samples treated with RNAlater solution and then frozen can be ground with mortar and pestle or thawed and processed like fresh tissue without concern for cell rupture and release of RNases since the RNases have already been inactivated Cells can be spun out and then added to lysis buffer or in some cases RNAlater solution can be added along with the cells directly to the lysis buffer Compatible with a variety of proceduresRNAlater RNA Stabilization Solution is compatible with one step RNA isolation methods such as TRIzol Reagent with glass binding methods such as Qiagen s RNeasy or the Ambion RNAqueous kit with acid phenol extraction methods such as the Ambion ToTALLY RNA kit and with methods that use oligo dT selection of mRNA such as the Ambion Poly A Purist kit In house research as well recently published independent research indicates that the use of RNAlater RNA Stabilization Solution for tissue storage does not affect the outcome of subsequent RNA expression analysis experiments compared to other processing methods
    Catalog Number:
    am7020
    Price:
    None
    Applications:
    DNA & RNA Purification & Analysis|General RNA Purification Reagents & Accessories|RNA Extraction|Total RNA Isolation|Total RNA from Animal Cells & Tissues|mRNA Isolation
    Category:
    Lab Reagents and Chemicals
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    Structured Review

    Thermo Fisher rnalater stabilization solution
    The RVC-based preservative solution has a milder effect on protein activity than 100% ethanol and <t>RNAlater.</t> (A-B) Determination of the effect of different preservative solutions on intracellular luciferase activity. The lysate of cells expressing luciferase was reacted with different preservative solutions, and luciferase activity was measured after 0, 0.5, 1, and 3 hours (n = 3). *** p
    RNAlater RNA Stabilization Solution stabilizes and protects cellular RNA in intact unfrozen tissue samples eliminating the need to immediately process tissue samples or to freeze samples in liquid nitrogen for later processing Tissue pieces can be harvested and submerged in RNAlater RNA Stabilization Solution for storage without jeopardizing the quality or quantity of RNA obtained after subsequent RNA isolation Advantages of using RNAlater RNA Stabilization Solution • Effectiveness stabilize RNA for 1 day at 37°C 1 week at 25°C 1 month at 4°C or indefinitely at 20°C• Simplicity a single reagent that immediately inactivates RNases and stabilizes RNA within tissues or cells• Convenience no need to freeze samples in liquid nitrogen or rush samples back to the lab freezer• Mobility perfect for tissue collection in the field • Versatility compatible with many RNA isolation procedures including most RNA isolation kitsApplicationsRNAlater RNA Stabilization Solution has been tested on a variety of mammalian tissues plants E coli Xenopus fish and Drosophila It is ideal for • Protecting RNA integrity in tissues rich in RNases• Collecting samples from different time points without having to process the samples from each time point immediately• Archiving tissues for future microdissection• Submerging animal cavities or organs to stabilize RNA during lengthy dissection procedures• Collecting samples at locations e g hospitals field sites the space shuttle where immediate RNA isolation is not possible• Shipping samples on wet ice or even at room temperature if shipped overnightRNAlater RNA Stabilization Solution procedureThe dissected tissue less than 0 5 cm in any one dimension is simply submerged in approximately 5 volumes of RNAlater solution at room temperature The solution permeates the cells stabilizing the RNA The sample can then be stored indefinitely at 20°C the tissue does not freeze at 4°C for up to a month or at 25°C for up to a week For RNA isolation the tissue is simply removed from RNAlater solution and treated as though it had just been harvested Most tissues can be transferred directly to a lysis buffer and homogenized Samples treated with RNAlater solution and then frozen can be ground with mortar and pestle or thawed and processed like fresh tissue without concern for cell rupture and release of RNases since the RNases have already been inactivated Cells can be spun out and then added to lysis buffer or in some cases RNAlater solution can be added along with the cells directly to the lysis buffer Compatible with a variety of proceduresRNAlater RNA Stabilization Solution is compatible with one step RNA isolation methods such as TRIzol Reagent with glass binding methods such as Qiagen s RNeasy or the Ambion RNAqueous kit with acid phenol extraction methods such as the Ambion ToTALLY RNA kit and with methods that use oligo dT selection of mRNA such as the Ambion Poly A Purist kit In house research as well recently published independent research indicates that the use of RNAlater RNA Stabilization Solution for tissue storage does not affect the outcome of subsequent RNA expression analysis experiments compared to other processing methods
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    rnalater stabilization solution - by Bioz Stars, 2020-02
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    Images

    1) Product Images from "Application of ribonucleoside vanadyl complex (RVC) for developing a multifunctional tissue preservative solution"

    Article Title: Application of ribonucleoside vanadyl complex (RVC) for developing a multifunctional tissue preservative solution

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0194393

    The RVC-based preservative solution has a milder effect on protein activity than 100% ethanol and RNAlater. (A-B) Determination of the effect of different preservative solutions on intracellular luciferase activity. The lysate of cells expressing luciferase was reacted with different preservative solutions, and luciferase activity was measured after 0, 0.5, 1, and 3 hours (n = 3). *** p
    Figure Legend Snippet: The RVC-based preservative solution has a milder effect on protein activity than 100% ethanol and RNAlater. (A-B) Determination of the effect of different preservative solutions on intracellular luciferase activity. The lysate of cells expressing luciferase was reacted with different preservative solutions, and luciferase activity was measured after 0, 0.5, 1, and 3 hours (n = 3). *** p

    Techniques Used: Activity Assay, Luciferase, Expressing

    Both the RVC-based preservative solution and RNAlater can effectively delay RNA degradation. (A-B) Quality of RNA in tissue samples (n = 3) preserved with different concentrations of RVC in the preservative solution at 4°C or −80°C for 7 days. (C) Quality of RNA in tissue samples (n = 5) preserved in different preservative solutions at 4°C for 7 days and −80°C for one month (D). NT: stored without any preservative solution; RVC+G: RVC+15% glycerol; RVC+D: RVC+5% DMSO; * p
    Figure Legend Snippet: Both the RVC-based preservative solution and RNAlater can effectively delay RNA degradation. (A-B) Quality of RNA in tissue samples (n = 3) preserved with different concentrations of RVC in the preservative solution at 4°C or −80°C for 7 days. (C) Quality of RNA in tissue samples (n = 5) preserved in different preservative solutions at 4°C for 7 days and −80°C for one month (D). NT: stored without any preservative solution; RVC+G: RVC+15% glycerol; RVC+D: RVC+5% DMSO; * p

    Techniques Used:

    RVC does not affect tissue morphology or the expression and distribution of proteins in tissue samples. (A) Morphological features of murine liver tissue samples stored in different preservative solutions. (B) The abovementioned paraffin-embedded tissue samples were sliced and stained with H E for the examination of cellular morphological characteristics. RNAlater and 100% ethanol caused significant tissue dehydration, whereas the RVC-based preservative solution did not cause this phenomenon. (C-D) Determination of EGFR and PCNA protein expression and distribution by immunohistochemical staining in tissue samples treated with different preservative solutions. The results from the RVC-based groups were consistent with the data of the control group.
    Figure Legend Snippet: RVC does not affect tissue morphology or the expression and distribution of proteins in tissue samples. (A) Morphological features of murine liver tissue samples stored in different preservative solutions. (B) The abovementioned paraffin-embedded tissue samples were sliced and stained with H E for the examination of cellular morphological characteristics. RNAlater and 100% ethanol caused significant tissue dehydration, whereas the RVC-based preservative solution did not cause this phenomenon. (C-D) Determination of EGFR and PCNA protein expression and distribution by immunohistochemical staining in tissue samples treated with different preservative solutions. The results from the RVC-based groups were consistent with the data of the control group.

    Techniques Used: Expressing, Staining, Immunohistochemistry

    2) Product Images from "Global and Quantitative Profiling of Polyadenylated RNAs Using PAS-seq"

    Article Title: Global and Quantitative Profiling of Polyadenylated RNAs Using PAS-seq

    Journal: Methods in molecular biology (Clifton, N.J.)

    doi: 10.1007/978-1-62703-971-0_16

    PAS-seq library. 10 % of the second-round PCR reaction (Subheading 3.6) is resolved on a 2 % agarose gel to check the size of DNA fragments
    Figure Legend Snippet: PAS-seq library. 10 % of the second-round PCR reaction (Subheading 3.6) is resolved on a 2 % agarose gel to check the size of DNA fragments

    Techniques Used: Polymerase Chain Reaction, Agarose Gel Electrophoresis

    3) Product Images from "Cch1 and Mid1 Are Functionally Required for Vegetative Growth under Low-Calcium Conditions in the Phytopathogenic Ascomycete Botrytis cinerea"

    Article Title: Cch1 and Mid1 Are Functionally Required for Vegetative Growth under Low-Calcium Conditions in the Phytopathogenic Ascomycete Botrytis cinerea

    Journal: Eukaryotic Cell

    doi: 10.1128/EC.00338-12

    Localization studies of the Mid1 protein in B. cinerea . (A) The Δ mid1 strain was transformed with a mid1 - gfp construct, where mid1 was fused N-terminally to gfp and was expressed under the control of the constitutively active oliC promoter. In germinated conidia (germination occurred for 20 h in GB5 medium in darkness), fluorescence was observed in network-like filaments and around the nuclei. Costaining of nuclei by Hoechst 33342 is depicted in Fig. S1 in the supplemental material. (B) The Δ mid1 strain was transformed with a gfp - mid1 construct, where mid1 was fused C-terminally to gfp and was expressed under the control of the constitutively active oliC promoter. The fluorescence signal observed was similar to that in panel A. (C) Staining of the Δ mid1 / mid1 - gfp strain with ER-Tracker. In the top three images, the intracellular GFP-fluorescent structures are mostly consistent with the signal of the ER marker (white), as shown in the overlay (merge; false color blue for ER-Tracker). At the bottom, a DIC image of the germinated conidia is shown. Scale bars, 10 μm.
    Figure Legend Snippet: Localization studies of the Mid1 protein in B. cinerea . (A) The Δ mid1 strain was transformed with a mid1 - gfp construct, where mid1 was fused N-terminally to gfp and was expressed under the control of the constitutively active oliC promoter. In germinated conidia (germination occurred for 20 h in GB5 medium in darkness), fluorescence was observed in network-like filaments and around the nuclei. Costaining of nuclei by Hoechst 33342 is depicted in Fig. S1 in the supplemental material. (B) The Δ mid1 strain was transformed with a gfp - mid1 construct, where mid1 was fused C-terminally to gfp and was expressed under the control of the constitutively active oliC promoter. The fluorescence signal observed was similar to that in panel A. (C) Staining of the Δ mid1 / mid1 - gfp strain with ER-Tracker. In the top three images, the intracellular GFP-fluorescent structures are mostly consistent with the signal of the ER marker (white), as shown in the overlay (merge; false color blue for ER-Tracker). At the bottom, a DIC image of the germinated conidia is shown. Scale bars, 10 μm.

    Techniques Used: Transformation Assay, Construct, Fluorescence, Staining, Marker

    4) Product Images from "Application of ribonucleoside vanadyl complex (RVC) for developing a multifunctional tissue preservative solution"

    Article Title: Application of ribonucleoside vanadyl complex (RVC) for developing a multifunctional tissue preservative solution

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0194393

    The RVC-based preservative solution has a milder effect on protein activity than 100% ethanol and RNAlater. (A-B) Determination of the effect of different preservative solutions on intracellular luciferase activity. The lysate of cells expressing luciferase was reacted with different preservative solutions, and luciferase activity was measured after 0, 0.5, 1, and 3 hours (n = 3). *** p
    Figure Legend Snippet: The RVC-based preservative solution has a milder effect on protein activity than 100% ethanol and RNAlater. (A-B) Determination of the effect of different preservative solutions on intracellular luciferase activity. The lysate of cells expressing luciferase was reacted with different preservative solutions, and luciferase activity was measured after 0, 0.5, 1, and 3 hours (n = 3). *** p

    Techniques Used: Activity Assay, Luciferase, Expressing

    Both the RVC-based preservative solution and RNAlater can effectively delay RNA degradation. (A-B) Quality of RNA in tissue samples (n = 3) preserved with different concentrations of RVC in the preservative solution at 4°C or −80°C for 7 days. (C) Quality of RNA in tissue samples (n = 5) preserved in different preservative solutions at 4°C for 7 days and −80°C for one month (D). NT: stored without any preservative solution; RVC+G: RVC+15% glycerol; RVC+D: RVC+5% DMSO; * p
    Figure Legend Snippet: Both the RVC-based preservative solution and RNAlater can effectively delay RNA degradation. (A-B) Quality of RNA in tissue samples (n = 3) preserved with different concentrations of RVC in the preservative solution at 4°C or −80°C for 7 days. (C) Quality of RNA in tissue samples (n = 5) preserved in different preservative solutions at 4°C for 7 days and −80°C for one month (D). NT: stored without any preservative solution; RVC+G: RVC+15% glycerol; RVC+D: RVC+5% DMSO; * p

    Techniques Used:

    5) Product Images from "Exploring the Genome and Phenotype of Multi-Drug Resistant Klebsiella pneumoniae of Clinical Origin"

    Article Title: Exploring the Genome and Phenotype of Multi-Drug Resistant Klebsiella pneumoniae of Clinical Origin

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2017.01913

    Determination of the ethidium bromide efflux by a semi-automated fluorometric method. Klebsiella pneumoniae isolates were saturated with 50 μM ethidium bromide and efflux measured by fluorometry at 37°C for 50 min with an excitation and emission wavelengths of 518- and 606-nm, in the presence or absence of glucose and CCCP. Black arrows indicate when glucose (50 mM) was added after 3 min of initial reading.
    Figure Legend Snippet: Determination of the ethidium bromide efflux by a semi-automated fluorometric method. Klebsiella pneumoniae isolates were saturated with 50 μM ethidium bromide and efflux measured by fluorometry at 37°C for 50 min with an excitation and emission wavelengths of 518- and 606-nm, in the presence or absence of glucose and CCCP. Black arrows indicate when glucose (50 mM) was added after 3 min of initial reading.

    Techniques Used:

    6) Product Images from "Novel molecular marker-assisted strategy for production of wheat–Leymus mollis chromosome addition lines"

    Article Title: Novel molecular marker-assisted strategy for production of wheat–Leymus mollis chromosome addition lines

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-34545-x

    Identification of L. mollis chromosomes added to wheat using genomic in situ hybridization (GISH). ( A – N ), L. mollis chromosomes; double letters, disomic lines; single letters, monosomic lines; arrows point to the added chromosomes detected with fluorescein-12-dUTP (green).
    Figure Legend Snippet: Identification of L. mollis chromosomes added to wheat using genomic in situ hybridization (GISH). ( A – N ), L. mollis chromosomes; double letters, disomic lines; single letters, monosomic lines; arrows point to the added chromosomes detected with fluorescein-12-dUTP (green).

    Techniques Used: In Situ Hybridization

    7) Product Images from "Matrix-bound nanovesicles within ECM bioscaffolds"

    Article Title: Matrix-bound nanovesicles within ECM bioscaffolds

    Journal: Science Advances

    doi: 10.1126/sciadv.1600502

    Identification of ECM-embedded MBVs. ( A ) TEM imaging of MBVs identified in a UBM sheet stained positive with osmium (left panel), pepsin-treated UBM (middle panel), or proteinase K–treated UBM (right panel). ( B ) TEM imaging of MBVs identified in proteinase K–treated ECM from three commercial and three laboratory-produced scaffolds. Scale bars, 100 nm. ( C ) Validation of MBV size was measured with NanoSight. ( D ) Western blot analysis was performed on four exosomal surface markers: CD63, CD81, CD9, and Hsp70. Expression levels were not detectable as compared to porcine serum, human serum, and human bone marrow–derived mesenchymal stem cell controls. ( E ) MBV protein cargo signature was different between MBVs and hMSCs as evaluated using SDS-PAGE and silver stain imaging.
    Figure Legend Snippet: Identification of ECM-embedded MBVs. ( A ) TEM imaging of MBVs identified in a UBM sheet stained positive with osmium (left panel), pepsin-treated UBM (middle panel), or proteinase K–treated UBM (right panel). ( B ) TEM imaging of MBVs identified in proteinase K–treated ECM from three commercial and three laboratory-produced scaffolds. Scale bars, 100 nm. ( C ) Validation of MBV size was measured with NanoSight. ( D ) Western blot analysis was performed on four exosomal surface markers: CD63, CD81, CD9, and Hsp70. Expression levels were not detectable as compared to porcine serum, human serum, and human bone marrow–derived mesenchymal stem cell controls. ( E ) MBV protein cargo signature was different between MBVs and hMSCs as evaluated using SDS-PAGE and silver stain imaging.

    Techniques Used: Transmission Electron Microscopy, Imaging, Staining, Produced, Western Blot, Expressing, Derivative Assay, SDS Page, Silver Staining

    Enzymatic digestion of decellularized ECM scaffolds releases small RNA molecules. ( A ) Nucleic acid extracted from untreated UBM (no digest) and pepsin-, proteinase K–, or collagenase-treated UBM was exposed to RNase A, DNase I, or no-nuclease treatment (control). ( B ) Electropherogram depicting the small RNA pattern of nucleic acid in fluorescence units (FU) before (top panel) and after (bottom panel) DNase I treatment. ( C ) Electropherogram depicting small RNA pattern from the indicated samples in FU. ( D ) A subset of nucleic molecules in biologic scaffolds is protected from nuclease degradation.
    Figure Legend Snippet: Enzymatic digestion of decellularized ECM scaffolds releases small RNA molecules. ( A ) Nucleic acid extracted from untreated UBM (no digest) and pepsin-, proteinase K–, or collagenase-treated UBM was exposed to RNase A, DNase I, or no-nuclease treatment (control). ( B ) Electropherogram depicting the small RNA pattern of nucleic acid in fluorescence units (FU) before (top panel) and after (bottom panel) DNase I treatment. ( C ) Electropherogram depicting small RNA pattern from the indicated samples in FU. ( D ) A subset of nucleic molecules in biologic scaffolds is protected from nuclease degradation.

    Techniques Used: Fluorescence

    Comparison of nucleic acid concentration from UBM, SIS, or dermis and their commercially available equivalents. ( A  to  C ) Concentration of total nucleic acid and dsDNA per milligram dry weight of ECM scaffold from untreated (control) and proteinase K– or collagenase-treated samples of (A) UBM and ACell MatriStem (porcine UBM), (B) SIS and Cook Biotech Biodesign (porcine SIS), and (C) dermis and C.R. Bard XenMatrix (porcine dermis). Total nucleic acid concentration was assessed by UV absorbance at 260 nm. dsDNA concentration was assessed using PicoGreen dsDNA quantification reagent. Variability from isolation to isolation is depicted by SD. Data are means ± SD;  n  = 3 isolations per sample.
    Figure Legend Snippet: Comparison of nucleic acid concentration from UBM, SIS, or dermis and their commercially available equivalents. ( A to C ) Concentration of total nucleic acid and dsDNA per milligram dry weight of ECM scaffold from untreated (control) and proteinase K– or collagenase-treated samples of (A) UBM and ACell MatriStem (porcine UBM), (B) SIS and Cook Biotech Biodesign (porcine SIS), and (C) dermis and C.R. Bard XenMatrix (porcine dermis). Total nucleic acid concentration was assessed by UV absorbance at 260 nm. dsDNA concentration was assessed using PicoGreen dsDNA quantification reagent. Variability from isolation to isolation is depicted by SD. Data are means ± SD; n = 3 isolations per sample.

    Techniques Used: Nucleic Acid Concentration, Concentration Assay, Isolation

    8) Product Images from "Application of ribonucleoside vanadyl complex (RVC) for developing a multifunctional tissue preservative solution"

    Article Title: Application of ribonucleoside vanadyl complex (RVC) for developing a multifunctional tissue preservative solution

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0194393

    The RVC-based preservative solution has a milder effect on protein activity than 100% ethanol and RNAlater. (A-B) Determination of the effect of different preservative solutions on intracellular luciferase activity. The lysate of cells expressing luciferase was reacted with different preservative solutions, and luciferase activity was measured after 0, 0.5, 1, and 3 hours (n = 3). *** p
    Figure Legend Snippet: The RVC-based preservative solution has a milder effect on protein activity than 100% ethanol and RNAlater. (A-B) Determination of the effect of different preservative solutions on intracellular luciferase activity. The lysate of cells expressing luciferase was reacted with different preservative solutions, and luciferase activity was measured after 0, 0.5, 1, and 3 hours (n = 3). *** p

    Techniques Used: Activity Assay, Luciferase, Expressing

    Both the RVC-based preservative solution and RNAlater can effectively delay RNA degradation. (A-B) Quality of RNA in tissue samples (n = 3) preserved with different concentrations of RVC in the preservative solution at 4°C or −80°C for 7 days. (C) Quality of RNA in tissue samples (n = 5) preserved in different preservative solutions at 4°C for 7 days and −80°C for one month (D). NT: stored without any preservative solution; RVC+G: RVC+15% glycerol; RVC+D: RVC+5% DMSO; * p
    Figure Legend Snippet: Both the RVC-based preservative solution and RNAlater can effectively delay RNA degradation. (A-B) Quality of RNA in tissue samples (n = 3) preserved with different concentrations of RVC in the preservative solution at 4°C or −80°C for 7 days. (C) Quality of RNA in tissue samples (n = 5) preserved in different preservative solutions at 4°C for 7 days and −80°C for one month (D). NT: stored without any preservative solution; RVC+G: RVC+15% glycerol; RVC+D: RVC+5% DMSO; * p

    Techniques Used:

    RVC does not affect tissue morphology or the expression and distribution of proteins in tissue samples. (A) Morphological features of murine liver tissue samples stored in different preservative solutions. (B) The abovementioned paraffin-embedded tissue samples were sliced and stained with H E for the examination of cellular morphological characteristics. RNAlater and 100% ethanol caused significant tissue dehydration, whereas the RVC-based preservative solution did not cause this phenomenon. (C-D) Determination of EGFR and PCNA protein expression and distribution by immunohistochemical staining in tissue samples treated with different preservative solutions. The results from the RVC-based groups were consistent with the data of the control group.
    Figure Legend Snippet: RVC does not affect tissue morphology or the expression and distribution of proteins in tissue samples. (A) Morphological features of murine liver tissue samples stored in different preservative solutions. (B) The abovementioned paraffin-embedded tissue samples were sliced and stained with H E for the examination of cellular morphological characteristics. RNAlater and 100% ethanol caused significant tissue dehydration, whereas the RVC-based preservative solution did not cause this phenomenon. (C-D) Determination of EGFR and PCNA protein expression and distribution by immunohistochemical staining in tissue samples treated with different preservative solutions. The results from the RVC-based groups were consistent with the data of the control group.

    Techniques Used: Expressing, Staining, Immunohistochemistry

    9) Product Images from "Application of ribonucleoside vanadyl complex (RVC) for developing a multifunctional tissue preservative solution"

    Article Title: Application of ribonucleoside vanadyl complex (RVC) for developing a multifunctional tissue preservative solution

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0194393

    The RVC-based preservative solution has a milder effect on protein activity than 100% ethanol and RNAlater. (A-B) Determination of the effect of different preservative solutions on intracellular luciferase activity. The lysate of cells expressing luciferase was reacted with different preservative solutions, and luciferase activity was measured after 0, 0.5, 1, and 3 hours (n = 3). *** p
    Figure Legend Snippet: The RVC-based preservative solution has a milder effect on protein activity than 100% ethanol and RNAlater. (A-B) Determination of the effect of different preservative solutions on intracellular luciferase activity. The lysate of cells expressing luciferase was reacted with different preservative solutions, and luciferase activity was measured after 0, 0.5, 1, and 3 hours (n = 3). *** p

    Techniques Used: Activity Assay, Luciferase, Expressing

    Both the RVC-based preservative solution and RNAlater can effectively delay RNA degradation. (A-B) Quality of RNA in tissue samples (n = 3) preserved with different concentrations of RVC in the preservative solution at 4°C or −80°C for 7 days. (C) Quality of RNA in tissue samples (n = 5) preserved in different preservative solutions at 4°C for 7 days and −80°C for one month (D). NT: stored without any preservative solution; RVC+G: RVC+15% glycerol; RVC+D: RVC+5% DMSO; * p
    Figure Legend Snippet: Both the RVC-based preservative solution and RNAlater can effectively delay RNA degradation. (A-B) Quality of RNA in tissue samples (n = 3) preserved with different concentrations of RVC in the preservative solution at 4°C or −80°C for 7 days. (C) Quality of RNA in tissue samples (n = 5) preserved in different preservative solutions at 4°C for 7 days and −80°C for one month (D). NT: stored without any preservative solution; RVC+G: RVC+15% glycerol; RVC+D: RVC+5% DMSO; * p

    Techniques Used:

    10) Product Images from "Rapid Regeneration and Reuse of Silica Columns from PCR Purification and Gel Extraction Kits"

    Article Title: Rapid Regeneration and Reuse of Silica Columns from PCR Purification and Gel Extraction Kits

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-30316-w

    Phosphoric acid is the most effective reagent for eliminating residual DNA from used Qiagen PCR purification kit columns. ( A ) A scheme showing the workflow for different protocols used in this study. ( B ) The standard curve that was plotted with the Ct values against –Log 10 DNA concentration (ng/μL). Ten microliters of LIF DNA template at a concentration of 100 ng/μL was consecutively diluted at 1/10 for 8 times, and the diluted samples (starting from 1 * 10 −2 ng/μL) were used for qPCR. Ct: Threshold cycles in quantitative PCR. ( C ) qPCR analysis to assess the amount of residual DNA eluted from the columns that were cleaned with different chemical reagents. LIF-contaminated columns were cleaned with indicated reagent based on protocol I. One microlitre of eluate was used to assess the presence of residual DNA by qPCR, with each sample assayed in triplicate. The obtained Ct values were converted into DNA concentration using the standard curve in B and were subjected to statistical analysis. The value of each column within the graph is presented in the bottom of the panel to clearly show the differences between the samples. ( D ) qPCR results for the amount of residual DNA from the columns that were cleaned with DNase I or acidic phenol. Contaminated columns were cleaned based on protocol II or III. qPCR assays were performed as in C. (E) qPCR showing DNA elimination for the used columns cleaned with different concentrations of phosphoric acid. Contaminated columns were cleaned with different concentrations of phosphoric acid as detailed in protocol I. qPCR assays performed as in C. Each column in C–E represents the mean ± SD of three independent experiments. * p
    Figure Legend Snippet: Phosphoric acid is the most effective reagent for eliminating residual DNA from used Qiagen PCR purification kit columns. ( A ) A scheme showing the workflow for different protocols used in this study. ( B ) The standard curve that was plotted with the Ct values against –Log 10 DNA concentration (ng/μL). Ten microliters of LIF DNA template at a concentration of 100 ng/μL was consecutively diluted at 1/10 for 8 times, and the diluted samples (starting from 1 * 10 −2 ng/μL) were used for qPCR. Ct: Threshold cycles in quantitative PCR. ( C ) qPCR analysis to assess the amount of residual DNA eluted from the columns that were cleaned with different chemical reagents. LIF-contaminated columns were cleaned with indicated reagent based on protocol I. One microlitre of eluate was used to assess the presence of residual DNA by qPCR, with each sample assayed in triplicate. The obtained Ct values were converted into DNA concentration using the standard curve in B and were subjected to statistical analysis. The value of each column within the graph is presented in the bottom of the panel to clearly show the differences between the samples. ( D ) qPCR results for the amount of residual DNA from the columns that were cleaned with DNase I or acidic phenol. Contaminated columns were cleaned based on protocol II or III. qPCR assays were performed as in C. (E) qPCR showing DNA elimination for the used columns cleaned with different concentrations of phosphoric acid. Contaminated columns were cleaned with different concentrations of phosphoric acid as detailed in protocol I. qPCR assays performed as in C. Each column in C–E represents the mean ± SD of three independent experiments. * p

    Techniques Used: Polymerase Chain Reaction, Purification, Concentration Assay, Real-time Polymerase Chain Reaction

    11) Product Images from "Leucine-rich diet induces a shift in tumour metabolism from glycolytic towards oxidative phosphorylation, reducing glucose consumption and metastasis in Walker-256 tumour-bearing rats"

    Article Title: Leucine-rich diet induces a shift in tumour metabolism from glycolytic towards oxidative phosphorylation, reducing glucose consumption and metastasis in Walker-256 tumour-bearing rats

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-52112-w

    In vitro assay of Walker-256 cell viability and superoxide production in response to leucine treatment. Approximately 1 × 10 3 W256 cells were seeded in 96-well plates and treated with 50 µM L-leucine for 24 ( A and C ) or 96 h ( B and D ). Cell viability was accessed using the neutral red uptake and the absorbance was normalised by the mean of a control group. Superoxide production was measured using dihydroethidium (DHE), and the fluorescence of each sample was normalised by the fluorescence of Hoechst 33342 (HO). Experiments were performed at least two times. Bar graphs represent the mean ± standard deviation. ***P
    Figure Legend Snippet: In vitro assay of Walker-256 cell viability and superoxide production in response to leucine treatment. Approximately 1 × 10 3 W256 cells were seeded in 96-well plates and treated with 50 µM L-leucine for 24 ( A and C ) or 96 h ( B and D ). Cell viability was accessed using the neutral red uptake and the absorbance was normalised by the mean of a control group. Superoxide production was measured using dihydroethidium (DHE), and the fluorescence of each sample was normalised by the fluorescence of Hoechst 33342 (HO). Experiments were performed at least two times. Bar graphs represent the mean ± standard deviation. ***P

    Techniques Used: In Vitro, Fluorescence, Standard Deviation

    12) Product Images from "Development of a Composite Measure of Product Adherence, Protocol Compliance, and Semen Exposure Using DNA and Protein Biomarkers for Topical HIV Prevention Studies"

    Article Title: Development of a Composite Measure of Product Adherence, Protocol Compliance, and Semen Exposure Using DNA and Protein Biomarkers for Topical HIV Prevention Studies

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0114368

    Isolation of DNA and Vaginal Cells from Inserted Gel Applicators. Vaginally inserted applicators or sham applicators were swabbed with a double headed rayon swab (1) to which one head was placed in a lysis buffer for DNA extraction and the other in Cytorich red for fixation of vaginal cells (2). The fixed vaginal cells were spotted onto slides for cytokeratin 4 immunohistochemistry (3).
    Figure Legend Snippet: Isolation of DNA and Vaginal Cells from Inserted Gel Applicators. Vaginally inserted applicators or sham applicators were swabbed with a double headed rayon swab (1) to which one head was placed in a lysis buffer for DNA extraction and the other in Cytorich red for fixation of vaginal cells (2). The fixed vaginal cells were spotted onto slides for cytokeratin 4 immunohistochemistry (3).

    Techniques Used: Isolation, Lysis, DNA Extraction, Immunohistochemistry

    Hematoxylin Stain Discriminates Vaginally Inserted from Sham Applicators. Swabbed cells from applicators were isolated and fixed in Cytorich Red solution. 5 µl of the resulting 20 µl suspensions from vaginally inserted applicators (A) and sham applicators (B) were spotted and dried overnight for staining the next day. The number of cells transferred from the hand to applicator during handling is too low to detect cells in a 5 µl aliquot (B).
    Figure Legend Snippet: Hematoxylin Stain Discriminates Vaginally Inserted from Sham Applicators. Swabbed cells from applicators were isolated and fixed in Cytorich Red solution. 5 µl of the resulting 20 µl suspensions from vaginally inserted applicators (A) and sham applicators (B) were spotted and dried overnight for staining the next day. The number of cells transferred from the hand to applicator during handling is too low to detect cells in a 5 µl aliquot (B).

    Techniques Used: Staining, Isolation

    13) Product Images from "Establishment of Epithelial Attachment on Titanium Surface Coated with Platelet Activating Peptide"

    Article Title: Establishment of Epithelial Attachment on Titanium Surface Coated with Platelet Activating Peptide

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0164693

    Adhesion of OBA9 epithelial cells to PRP pre-incubated titanium surfaces. Adhered OBA9 cells were counted by nuclear DNA staining with Hoechst 33342. Each data point represents mean ± SD of three independent cultures. Kinetic adhesion was determined in the titanium that was pre-incubated with PRP from one representative donor (a). The adhesion assay was triplicated at 60 minutes with PRP from different donors (b). ** indicates a significant difference than control at the corresponding time point and in the corresponding donors (** p
    Figure Legend Snippet: Adhesion of OBA9 epithelial cells to PRP pre-incubated titanium surfaces. Adhered OBA9 cells were counted by nuclear DNA staining with Hoechst 33342. Each data point represents mean ± SD of three independent cultures. Kinetic adhesion was determined in the titanium that was pre-incubated with PRP from one representative donor (a). The adhesion assay was triplicated at 60 minutes with PRP from different donors (b). ** indicates a significant difference than control at the corresponding time point and in the corresponding donors (** p

    Techniques Used: Incubation, Staining, Cell Adhesion Assay

    14) Product Images from "Two design strategies for enhancement of multilayer-DNA-origami folding: underwinding for specific intercalator rescue and staple-break positioning"

    Article Title: Two design strategies for enhancement of multilayer-DNA-origami folding: underwinding for specific intercalator rescue and staple-break positioning

    Journal: Chemical science (Royal Society of Chemistry : 2010)

    doi: 10.1039/C2SC20446K

    Specific PEG-tris-acridine functionalization by multivalent intercalation into underwound DNA origami (A) Design schematic of PEG-tris-acridine targeting the underwound helices of the twelve-helix DNA bundle. Left, model views off perpendicular and along to the helical axis of 200 nm-long twelve-helix DNA bundles with 10.5 and 11 bp/turn reciprocal double-helical twist density. Right, model of the multivalent intercalation between underwound twelve-helix DNA bundle and synthesized PEG-tris-acridine. (B) Gel-mobility-shift assays using Mg-agarose electrophoresis following the DNA binding with PEG-tris-acridine at different weight ratios. The gels were stained by ethidium bromide after 1% (w/v) agarose gel electrophoresis. In green, the ethidium fluorescence from the DNA and in red, the acridine fluorescence from the PEG-tris-acridine. Lanes 1 to 5 show the retention of the p8064 M13-derived ssDNA at various PEG-tris-acridine to DNA weight ratios of 0:1, 2:1, 5:1, 10:1, 15:1. Lane 6, 6hb_10.5 bp/turn without PEG-tris-acridine. Lane 7, 6hb_10.5 bp/turn with PEG-tris-acridine at 60 µM. Lane 8, 12hb_10.5 bp/turn without PEG-tris-acridine. Lane 9, 12hb_10.5 bp/turn with PEG-tris-acridine at 60 µM. Lane 10, 1 kb ladder. Lane 11, underwound 12hb_11 bp/turn without PEG-tris-acridine. Lanes 12 to 16 show the retention of underwound 12hb_11 bp/turn at various PEG-tris-acridine to DNA weight ratio of 3:2, 4:1, 6:1, 8:1, 12:1. Lane 17,1 kb ladder. Lane 18, 6hb_ring. Lanes 19 to 23 show the retention of 6hb_ring at various PEG-tris-acridine to DNA weight ratios from 3:2, 4:1, 6:1, 8:1, 12:1. (C) TEM images of unPEGylated and PEGylated DNA nanostructures. 1, 12hb_10.5 bp/turn extracted from gels and 2, zoom in images. 3, 12hb_11 bp/turn extracted from gels and 4, zoom in images. 5, PEGylated 12hb_11 bp/turn, 6, zoom in images and 7, stained with 0.5% Ruthenium tetroxide. 8, 6hb_ring unPEGylated. 9, 6hb_ring PEGylated. Scale bars are 50 nm.
    Figure Legend Snippet: Specific PEG-tris-acridine functionalization by multivalent intercalation into underwound DNA origami (A) Design schematic of PEG-tris-acridine targeting the underwound helices of the twelve-helix DNA bundle. Left, model views off perpendicular and along to the helical axis of 200 nm-long twelve-helix DNA bundles with 10.5 and 11 bp/turn reciprocal double-helical twist density. Right, model of the multivalent intercalation between underwound twelve-helix DNA bundle and synthesized PEG-tris-acridine. (B) Gel-mobility-shift assays using Mg-agarose electrophoresis following the DNA binding with PEG-tris-acridine at different weight ratios. The gels were stained by ethidium bromide after 1% (w/v) agarose gel electrophoresis. In green, the ethidium fluorescence from the DNA and in red, the acridine fluorescence from the PEG-tris-acridine. Lanes 1 to 5 show the retention of the p8064 M13-derived ssDNA at various PEG-tris-acridine to DNA weight ratios of 0:1, 2:1, 5:1, 10:1, 15:1. Lane 6, 6hb_10.5 bp/turn without PEG-tris-acridine. Lane 7, 6hb_10.5 bp/turn with PEG-tris-acridine at 60 µM. Lane 8, 12hb_10.5 bp/turn without PEG-tris-acridine. Lane 9, 12hb_10.5 bp/turn with PEG-tris-acridine at 60 µM. Lane 10, 1 kb ladder. Lane 11, underwound 12hb_11 bp/turn without PEG-tris-acridine. Lanes 12 to 16 show the retention of underwound 12hb_11 bp/turn at various PEG-tris-acridine to DNA weight ratio of 3:2, 4:1, 6:1, 8:1, 12:1. Lane 17,1 kb ladder. Lane 18, 6hb_ring. Lanes 19 to 23 show the retention of 6hb_ring at various PEG-tris-acridine to DNA weight ratios from 3:2, 4:1, 6:1, 8:1, 12:1. (C) TEM images of unPEGylated and PEGylated DNA nanostructures. 1, 12hb_10.5 bp/turn extracted from gels and 2, zoom in images. 3, 12hb_11 bp/turn extracted from gels and 4, zoom in images. 5, PEGylated 12hb_11 bp/turn, 6, zoom in images and 7, stained with 0.5% Ruthenium tetroxide. 8, 6hb_ring unPEGylated. 9, 6hb_ring PEGylated. Scale bars are 50 nm.

    Techniques Used: Synthesized, Mobility Shift, Electrophoresis, Binding Assay, Staining, Agarose Gel Electrophoresis, Fluorescence, Derivative Assay, Transmission Electron Microscopy

    15) Product Images from "Rapid Regeneration and Reuse of Silica Columns from PCR Purification and Gel Extraction Kits"

    Article Title: Rapid Regeneration and Reuse of Silica Columns from PCR Purification and Gel Extraction Kits

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-30316-w

    Phosphoric acid is the most effective reagent for eliminating residual DNA from used Qiagen PCR purification kit columns. ( A ) A scheme showing the workflow for different protocols used in this study. ( B ) The standard curve that was plotted with the Ct values against –Log 10 DNA concentration (ng/μL). Ten microliters of LIF DNA template at a concentration of 100 ng/μL was consecutively diluted at 1/10 for 8 times, and the diluted samples (starting from 1 * 10 −2 ng/μL) were used for qPCR. Ct: Threshold cycles in quantitative PCR. ( C ) qPCR analysis to assess the amount of residual DNA eluted from the columns that were cleaned with different chemical reagents. LIF-contaminated columns were cleaned with indicated reagent based on protocol I. One microlitre of eluate was used to assess the presence of residual DNA by qPCR, with each sample assayed in triplicate. The obtained Ct values were converted into DNA concentration using the standard curve in B and were subjected to statistical analysis. The value of each column within the graph is presented in the bottom of the panel to clearly show the differences between the samples. ( D ) qPCR results for the amount of residual DNA from the columns that were cleaned with DNase I or acidic phenol. Contaminated columns were cleaned based on protocol II or III. qPCR assays were performed as in C. (E) qPCR showing DNA elimination for the used columns cleaned with different concentrations of phosphoric acid. Contaminated columns were cleaned with different concentrations of phosphoric acid as detailed in protocol I. qPCR assays performed as in C. Each column in C–E represents the mean ± SD of three independent experiments. * p
    Figure Legend Snippet: Phosphoric acid is the most effective reagent for eliminating residual DNA from used Qiagen PCR purification kit columns. ( A ) A scheme showing the workflow for different protocols used in this study. ( B ) The standard curve that was plotted with the Ct values against –Log 10 DNA concentration (ng/μL). Ten microliters of LIF DNA template at a concentration of 100 ng/μL was consecutively diluted at 1/10 for 8 times, and the diluted samples (starting from 1 * 10 −2 ng/μL) were used for qPCR. Ct: Threshold cycles in quantitative PCR. ( C ) qPCR analysis to assess the amount of residual DNA eluted from the columns that were cleaned with different chemical reagents. LIF-contaminated columns were cleaned with indicated reagent based on protocol I. One microlitre of eluate was used to assess the presence of residual DNA by qPCR, with each sample assayed in triplicate. The obtained Ct values were converted into DNA concentration using the standard curve in B and were subjected to statistical analysis. The value of each column within the graph is presented in the bottom of the panel to clearly show the differences between the samples. ( D ) qPCR results for the amount of residual DNA from the columns that were cleaned with DNase I or acidic phenol. Contaminated columns were cleaned based on protocol II or III. qPCR assays were performed as in C. (E) qPCR showing DNA elimination for the used columns cleaned with different concentrations of phosphoric acid. Contaminated columns were cleaned with different concentrations of phosphoric acid as detailed in protocol I. qPCR assays performed as in C. Each column in C–E represents the mean ± SD of three independent experiments. * p

    Techniques Used: Polymerase Chain Reaction, Purification, Concentration Assay, Real-time Polymerase Chain Reaction

    16) Product Images from "Optimized Scorpion Polypeptide LMX: A Pest Control Protein Effective against Rice Leaf Folder"

    Article Title: Optimized Scorpion Polypeptide LMX: A Pest Control Protein Effective against Rice Leaf Folder

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0100232

    Molecular analysis of the LMX transgenic lines. A . Schematic representation of the transgenic binary vector pCAMBIA 1301-LMX. B . Analysis of T 0 independent transgenic plants expressing LMX by PCR. M, DL2000 marker; 1, Yuetai; 2, ddH 2 O; 3–12, T 0 generation of transgenic plants expressing LMX gene. C . Southern-blot analysis of T 0 transgenic lines with single copy insert. The DNA samples were hybridized with the prepared biotin-11-dUTP probe. D . qRT-PCR analysis of LMX expression in transgenic lines L-5, L-10, and L-22. The ubiquitin gene was used as the internal control. E . Western blot assay of T 2 homozygous transgenic line L-10 using LMX antibody. P, purified LMX protein; 1, Yuetai; 2, empty vector transgenic line rbcS; 3–7, homozygous T 2 LMX-10 transgenic lines.
    Figure Legend Snippet: Molecular analysis of the LMX transgenic lines. A . Schematic representation of the transgenic binary vector pCAMBIA 1301-LMX. B . Analysis of T 0 independent transgenic plants expressing LMX by PCR. M, DL2000 marker; 1, Yuetai; 2, ddH 2 O; 3–12, T 0 generation of transgenic plants expressing LMX gene. C . Southern-blot analysis of T 0 transgenic lines with single copy insert. The DNA samples were hybridized with the prepared biotin-11-dUTP probe. D . qRT-PCR analysis of LMX expression in transgenic lines L-5, L-10, and L-22. The ubiquitin gene was used as the internal control. E . Western blot assay of T 2 homozygous transgenic line L-10 using LMX antibody. P, purified LMX protein; 1, Yuetai; 2, empty vector transgenic line rbcS; 3–7, homozygous T 2 LMX-10 transgenic lines.

    Techniques Used: Transgenic Assay, Plasmid Preparation, Expressing, Polymerase Chain Reaction, Marker, Southern Blot, Quantitative RT-PCR, Western Blot, Purification

    17) Product Images from "Novel molecular marker-assisted strategy for production of wheat–Leymus mollis chromosome addition lines"

    Article Title: Novel molecular marker-assisted strategy for production of wheat–Leymus mollis chromosome addition lines

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-34545-x

    Identification of L. mollis chromosomes added to wheat using genomic in situ hybridization (GISH). ( A – N ), L. mollis chromosomes; double letters, disomic lines; single letters, monosomic lines; arrows point to the added chromosomes detected with fluorescein-12-dUTP (green).
    Figure Legend Snippet: Identification of L. mollis chromosomes added to wheat using genomic in situ hybridization (GISH). ( A – N ), L. mollis chromosomes; double letters, disomic lines; single letters, monosomic lines; arrows point to the added chromosomes detected with fluorescein-12-dUTP (green).

    Techniques Used: In Situ Hybridization

    Related Articles

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    Synthesized:

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    Activity Assay:

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    Infection:

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    Expressing:

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    High Performance Liquid Chromatography:

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    Dissection:

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    Northern Blot:

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    Cell Culture:

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    Reverse Transcription Polymerase Chain Reaction:

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    DNA Extraction:

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    Fluorescence:

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    Isolation:

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    RNA Extraction:

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    Purification:

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    Article Snippet: Cell culture Cells were cultured and treated in 8-well plates (SCC-1, SCC-9, CAL-27, SCC-6, SCC-47, SCC-104, SCC-90 and SCC-152) and washed with 3 × 2 ml PBS, with the exception of UM-SCC-6 which were washed only 1 × 2 ml, cells were scraped, and transferred in 300 μL RNA Later (cat: AM7021, ThermoFisher Scientific) to 1.7 mL centrifuge tubes. .. RNA purification was performed using the RNeasy Plus Mini Kit supplemented with gDNA Eliminator mini Spin Columns (Cat#: 74134 Qiagen) and QIAshredder (Cat#: 79656 Qiagen) per manufacturers guidelines.

    Article Title: Genome‐wide identification of urinary cell‐free micro RNAs for non‐invasive detection of bladder cancer
    Article Snippet: Urine samples were centrifuged again before RNA isolation at 4°C at 12,000 g for 15 min. Total RNA from 1 ml of cell‐free urine supernatant was isolated using Urine microRNA Purification Kit (Norgen Biotek, Thorold, ON, Canada). .. Tumour tissue and adjacent bladder non‐tumour tissue were removed within transurethral resection of tumour or radical cystectomy, placed in in RNAlater™ Stabilization Solution (Invitrogen by Thermo Fisher Scientific, Waltham, MA, USA) and stored at −80°C until analysed.

    Article Title: EGFR as a biomarker of smoking status and survival in oropharyngeal squamous cell carcinoma
    Article Snippet: Cells were cultured and treated in 8-well plates (SCC-1, SCC-9, CAL-27, SCC-6, SCC-47, SCC-104, SCC-90 and SCC-152) and washed with 3 × 2 ml PBS, with the exception of UM-SCC-6 which were washed only 1 × 2 ml, cells were scraped, and transferred in 300 μL RNA Later (cat: AM7021, ThermoFisher Scientific) to 1.7 mL centrifuge tubes. .. RNA purification was performed using the RNeasy Plus Mini Kit supplemented with gDNA Eliminator mini Spin Columns (Cat#: 74134 Qiagen) and QIAshredder (Cat#: 79656 Qiagen) per manufacturers guidelines.

    Transgenic Assay:

    Article Title: Normal Development in Mice Over-Expressing the Intracellular Domain of DLL1 Argues against Reverse Signaling by DLL1 In Vivo
    Article Snippet: .. RNA preparation from embryos and cDNA synthesis E9.5 17-19 somite stage transgenic embryos (D ICD, fDICD Flag and DΔECD) were individually transferred into RNAlater (Ambion,#AM7020) and RNA was isolated using TriReagent (Sigma, #T9424) according to the manufacturer`s protocol. cDNA was synthesized from one quarter of each RNA preparation using the Thermoscript RT-PCR System (Invitrogen, #11146-016) according to the manufacturer`s protocol. .. Quantitative real-time PCR qRT-PCR was performed with Platinum TaqPCRx DNA Polymerase (Invitrogen, #11509-015) according to the basic protocol with SYBR Green and Rox (Invitrogen, #12223-012) in a 25µl reaction volume in a 7500 Fast Real-Time PCR System (Applied Biosystems) in duplicate.

    Quantitative RT-PCR:

    Article Title: EGFR as a biomarker of smoking status and survival in oropharyngeal squamous cell carcinoma
    Article Snippet: Cell culture Cells were cultured and treated in 8-well plates (SCC-1, SCC-9, CAL-27, SCC-6, SCC-47, SCC-104, SCC-90 and SCC-152) and washed with 3 × 2 ml PBS, with the exception of UM-SCC-6 which were washed only 1 × 2 ml, cells were scraped, and transferred in 300 μL RNA Later (cat: AM7021, ThermoFisher Scientific) to 1.7 mL centrifuge tubes. .. RNA was used to synthesize cDNA using the iScriptTM Reverse Transcription Supermix for RT-qPCR (Cat#: 1708841 BIO-RAD) as per the manufacturer’s guidelines.

    Article Title: Prostate Apoptosis Response-4 Is Expressed in Normal Cholangiocytes, Is Down-Regulated in Human Cholangiocarcinoma, and Promotes Apoptosis of Neoplastic Cholangiocytes When Induced Pharmacologically
    Article Snippet: Paragraph title: Quantitative RT-PCR ... Pieces of dissected tissues (about 30 mg) were immediately submerged in RNAlater stabilization solution (Ambion, Inc., Austin, TX).

    Article Title: EGFR as a biomarker of smoking status and survival in oropharyngeal squamous cell carcinoma
    Article Snippet: Cells were cultured and treated in 8-well plates (SCC-1, SCC-9, CAL-27, SCC-6, SCC-47, SCC-104, SCC-90 and SCC-152) and washed with 3 × 2 ml PBS, with the exception of UM-SCC-6 which were washed only 1 × 2 ml, cells were scraped, and transferred in 300 μL RNA Later (cat: AM7021, ThermoFisher Scientific) to 1.7 mL centrifuge tubes. .. RNA was used to synthesize cDNA using the iScriptTM Reverse Transcription Supermix for RT-qPCR (Cat#: 1708841 BIO-RAD) as per the manufacturer’s guidelines.

    Chromatin Immunoprecipitation:

    Article Title: De novo transcriptome analysis of the excretory tubules of Carausius morosus (Phasmatodea) and possible functions of the midgut ‘appendices’
    Article Snippet: Tissues (approximately 20μg each) were then immediately placed into Invitrogen™ RNAlater™ Stabilization Solution (ThermoFischer) and macerated in a frozen Tissue Lyser with metal beads. .. RNA quality was tested with an Experion™ RNA chip (Bio-Rad) on an Agilent 2100 Bioanalyzer following the manufacturers’ protocols.

    Functional Assay:

    Article Title: Tissue microenvironment dictates the fate and tumor-suppressive function of type 3 ILCs
    Article Snippet: NGS For NGS, tumor tissue was resected 5 d after tumor inoculation and was immediately transferred into RNAlater stabilization solution (Ambion). .. NGS was performed by the Functional Genomic Center Zurich ( http://www.fgcz.ch ) using the HiSeq 2500 v4 System (Illumina).

    Next-Generation Sequencing:

    Article Title: Tissue microenvironment dictates the fate and tumor-suppressive function of type 3 ILCs
    Article Snippet: .. NGS For NGS, tumor tissue was resected 5 d after tumor inoculation and was immediately transferred into RNAlater stabilization solution (Ambion). .. For total RNA isolation, the tumor tissue was homogenized using a tissue homogenizer (Omni International), and total RNA was extracted using the RNeasy Micro Kit Plus (QIAGEN) according to the manufacturer’s instructions.

    Preserving:

    Article Title: Genome‐wide identification of urinary cell‐free micro RNAs for non‐invasive detection of bladder cancer
    Article Snippet: Samples of first morning voided urine were collected in 15 ml tubes with EDTA used for nucleic acid preservation, centrifuged at 4°C at 2000 g for 15 min. Supernatant was collected and stored at ‐80°C until analysed. .. Tumour tissue and adjacent bladder non‐tumour tissue were removed within transurethral resection of tumour or radical cystectomy, placed in in RNAlater™ Stabilization Solution (Invitrogen by Thermo Fisher Scientific, Waltham, MA, USA) and stored at −80°C until analysed.

    Spectrophotometry:

    Article Title: Genome‐wide identification of urinary cell‐free micro RNAs for non‐invasive detection of bladder cancer
    Article Snippet: Tumour tissue and adjacent bladder non‐tumour tissue were removed within transurethral resection of tumour or radical cystectomy, placed in in RNAlater™ Stabilization Solution (Invitrogen by Thermo Fisher Scientific, Waltham, MA, USA) and stored at −80°C until analysed. .. Quality and quantity of RNA were determined using the NanoDrop™ 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA).

    Sampling:

    Article Title: Evaluation of potential reference genes for real-time qPCR analysis in a biparental beetle, Lethrus apterus (Coleoptera: Geotrupidae)
    Article Snippet: On each sampling dates, head and thorax samples were collected from eight males and eight females. .. Each head and thorax sample was put immediately into separate eppendorf tubes which already contained 600 µl RNAlater ® Stabilization Solution (Thermo Fisher Scientific, Waltham, MA, USA), then stored at −20 °C in the laboratory in order to inhibit RNase enzyme activity until RNA extraction.

    Article Title: Fine-scale spatial and temporal dynamics of kdr haplotypes in Aedes aegypti from Mexico
    Article Snippet: We identified each mosquito to the species level and sex, and Ae. aegypti were stored in RNAlater ® Stabilization Solution (Thermo Fisher Scientific, Waltham, MA, USA) for future kdr genotyping. .. To augment sampling efforts and supplement Prokopack collections, we placed oviposition traps (ovitraps) outside four houses per block (one on each side of the block) for up to 4 weeks to collect Ae. aegypti eggs.

    Article Title: Insights into teleost sex determination from the Seriola dorsalis genome assembly
    Article Snippet: The whole juvenile fish was then placed immediately into RNAlater ® Stabilization Solution (AMBION, Thermo Fisher Scientific, Waltham, MA), stored for 24 h at 4 °C and then frozen at −20 °C until DNA extraction. .. Tissue specimens were acquired via hook and line sampling by private sport anglers or commercial/subsistence fishers aboard various fishing vessels, scientific observers then sampled these specimens shipboard or at landing docks.

    Concentration Assay:

    Article Title: Insights into teleost sex determination from the Seriola dorsalis genome assembly
    Article Snippet: This individual was humanely euthanized by placing the fish in a bath containing a lethal overdose (a concentration of 800 mg/L) of the anesthetic tricaine methanesulfonate (MS-222). .. The whole juvenile fish was then placed immediately into RNAlater ® Stabilization Solution (AMBION, Thermo Fisher Scientific, Waltham, MA), stored for 24 h at 4 °C and then frozen at −20 °C until DNA extraction.

    Lysis:

    Article Title: Renal compartment-specific genetic variation analyses identify new pathways in chronic kidney disease
    Article Snippet: Collected tissue was immersed in RNAlater (Ambion#AM7020) solution at 4°C for several hours prior to being stored at −80°C in RNAlater. .. In general, 60–150 glomeruli that readily released from the capsule were collected and placed into RNeasy RNA Tissue Lysis Buffer Solution (per Qiagen RNeasy kit manufacturer instructions (Qiagen#74106)).

    Fluorescence In Situ Hybridization:

    Article Title: Insights into teleost sex determination from the Seriola dorsalis genome assembly
    Article Snippet: .. The whole juvenile fish was then placed immediately into RNAlater ® Stabilization Solution (AMBION, Thermo Fisher Scientific, Waltham, MA), stored for 24 h at 4 °C and then frozen at −20 °C until DNA extraction. ..

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    Thermo Fisher rnalater stabilization solution
    The RVC-based preservative solution has a milder effect on protein activity than 100% ethanol and <t>RNAlater.</t> (A-B) Determination of the effect of different preservative solutions on intracellular luciferase activity. The lysate of cells expressing luciferase was reacted with different preservative solutions, and luciferase activity was measured after 0, 0.5, 1, and 3 hours (n = 3). *** p
    Rnalater Stabilization Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 139 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The RVC-based preservative solution has a milder effect on protein activity than 100% ethanol and RNAlater. (A-B) Determination of the effect of different preservative solutions on intracellular luciferase activity. The lysate of cells expressing luciferase was reacted with different preservative solutions, and luciferase activity was measured after 0, 0.5, 1, and 3 hours (n = 3). *** p

    Journal: PLoS ONE

    Article Title: Application of ribonucleoside vanadyl complex (RVC) for developing a multifunctional tissue preservative solution

    doi: 10.1371/journal.pone.0194393

    Figure Lengend Snippet: The RVC-based preservative solution has a milder effect on protein activity than 100% ethanol and RNAlater. (A-B) Determination of the effect of different preservative solutions on intracellular luciferase activity. The lysate of cells expressing luciferase was reacted with different preservative solutions, and luciferase activity was measured after 0, 0.5, 1, and 3 hours (n = 3). *** p

    Article Snippet: In this study, we found that the RVC-based preservative solution causes less tissue damage than the more widely used RNAlater® Stabilization Solution.

    Techniques: Activity Assay, Luciferase, Expressing

    Both the RVC-based preservative solution and RNAlater can effectively delay RNA degradation. (A-B) Quality of RNA in tissue samples (n = 3) preserved with different concentrations of RVC in the preservative solution at 4°C or −80°C for 7 days. (C) Quality of RNA in tissue samples (n = 5) preserved in different preservative solutions at 4°C for 7 days and −80°C for one month (D). NT: stored without any preservative solution; RVC+G: RVC+15% glycerol; RVC+D: RVC+5% DMSO; * p

    Journal: PLoS ONE

    Article Title: Application of ribonucleoside vanadyl complex (RVC) for developing a multifunctional tissue preservative solution

    doi: 10.1371/journal.pone.0194393

    Figure Lengend Snippet: Both the RVC-based preservative solution and RNAlater can effectively delay RNA degradation. (A-B) Quality of RNA in tissue samples (n = 3) preserved with different concentrations of RVC in the preservative solution at 4°C or −80°C for 7 days. (C) Quality of RNA in tissue samples (n = 5) preserved in different preservative solutions at 4°C for 7 days and −80°C for one month (D). NT: stored without any preservative solution; RVC+G: RVC+15% glycerol; RVC+D: RVC+5% DMSO; * p

    Article Snippet: In this study, we found that the RVC-based preservative solution causes less tissue damage than the more widely used RNAlater® Stabilization Solution.

    Techniques:

    RVC does not affect tissue morphology or the expression and distribution of proteins in tissue samples. (A) Morphological features of murine liver tissue samples stored in different preservative solutions. (B) The abovementioned paraffin-embedded tissue samples were sliced and stained with H E for the examination of cellular morphological characteristics. RNAlater and 100% ethanol caused significant tissue dehydration, whereas the RVC-based preservative solution did not cause this phenomenon. (C-D) Determination of EGFR and PCNA protein expression and distribution by immunohistochemical staining in tissue samples treated with different preservative solutions. The results from the RVC-based groups were consistent with the data of the control group.

    Journal: PLoS ONE

    Article Title: Application of ribonucleoside vanadyl complex (RVC) for developing a multifunctional tissue preservative solution

    doi: 10.1371/journal.pone.0194393

    Figure Lengend Snippet: RVC does not affect tissue morphology or the expression and distribution of proteins in tissue samples. (A) Morphological features of murine liver tissue samples stored in different preservative solutions. (B) The abovementioned paraffin-embedded tissue samples were sliced and stained with H E for the examination of cellular morphological characteristics. RNAlater and 100% ethanol caused significant tissue dehydration, whereas the RVC-based preservative solution did not cause this phenomenon. (C-D) Determination of EGFR and PCNA protein expression and distribution by immunohistochemical staining in tissue samples treated with different preservative solutions. The results from the RVC-based groups were consistent with the data of the control group.

    Article Snippet: In this study, we found that the RVC-based preservative solution causes less tissue damage than the more widely used RNAlater® Stabilization Solution.

    Techniques: Expressing, Staining, Immunohistochemistry