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    Thermo Fisher dna ladders
    Generation and testing of a conditional (“floxed”) tbx20 allele. a . Experimental design. b . Genotyping of adult fish for intron 2 loxP site. c . Results of nested PCR screening for loxP integration into intron 1. d . Sequence of intron 1 loxP integration in recovered tpl145 floxed tbx20 allele. e . Genotyping of “F1” adults. Primer binding sites are shown as black arrows, loxP sites as red or blue triangles, exon as an open box. f, g . Induction of tbx20 loss of function by injection of Cre mRNA. f . One quarter of embryos obtained by in-crossing tbx20 tpl145 heterozygotes and injected with Cre mRNA display a consistent, severe heart development defect. g . Genotyping of embryos with severe heart defects (lanes 1–3) and phenotypically normal siblings (lanes 4–8). L, <t>GeneRuler</t> <t>DNA</t> Ladder (ThermoFisher Scientific). Genotyping lanes 1 and 5 correspond to images in f . h, i . Induction of tbx20 deletion by 4-hydroxytamoxifen. tbx20 tpl145 heterozygote was crossed to Tg( ubi :CreERT2) line. GFP-positive embryos were collected at 2dpf and incubated with 5μM 4-HT for 24 hours. j . Adults raised from Cre-injected tbx20 tpl145/+ embryos were incrossed, resulting in approximately 1/4 of embryos (36/128, 28%) with severe heart defects. k . Confirmation of Cre-mediated excision of the second intron of tbx20 by sequence analysis. l, m . Analysis of excision efficiency by qPCR. l . Excision efficiency was assessed by qPCR in embryos treated with 4-HT at various concentrations at different time points and in two different ubb :CreERT2 driver lines. The y-axis indicates un-excised tpl 145 normalized to the untreated control. m . Images of tbx20 phenotypes shown after treatment with 4-HT. Individuals 1, 2, and 3 from l correspond to images labeled 1, 2, and 3.
    Dna Ladders, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Conditional mutagenesis by oligonucleotide-mediated integration of loxP sites in zebrafish"

    Article Title: Conditional mutagenesis by oligonucleotide-mediated integration of loxP sites in zebrafish

    Journal: PLoS Genetics

    doi: 10.1371/journal.pgen.1007754

    Generation and testing of a conditional (“floxed”) tbx20 allele. a . Experimental design. b . Genotyping of adult fish for intron 2 loxP site. c . Results of nested PCR screening for loxP integration into intron 1. d . Sequence of intron 1 loxP integration in recovered tpl145 floxed tbx20 allele. e . Genotyping of “F1” adults. Primer binding sites are shown as black arrows, loxP sites as red or blue triangles, exon as an open box. f, g . Induction of tbx20 loss of function by injection of Cre mRNA. f . One quarter of embryos obtained by in-crossing tbx20 tpl145 heterozygotes and injected with Cre mRNA display a consistent, severe heart development defect. g . Genotyping of embryos with severe heart defects (lanes 1–3) and phenotypically normal siblings (lanes 4–8). L, GeneRuler DNA Ladder (ThermoFisher Scientific). Genotyping lanes 1 and 5 correspond to images in f . h, i . Induction of tbx20 deletion by 4-hydroxytamoxifen. tbx20 tpl145 heterozygote was crossed to Tg( ubi :CreERT2) line. GFP-positive embryos were collected at 2dpf and incubated with 5μM 4-HT for 24 hours. j . Adults raised from Cre-injected tbx20 tpl145/+ embryos were incrossed, resulting in approximately 1/4 of embryos (36/128, 28%) with severe heart defects. k . Confirmation of Cre-mediated excision of the second intron of tbx20 by sequence analysis. l, m . Analysis of excision efficiency by qPCR. l . Excision efficiency was assessed by qPCR in embryos treated with 4-HT at various concentrations at different time points and in two different ubb :CreERT2 driver lines. The y-axis indicates un-excised tpl 145 normalized to the untreated control. m . Images of tbx20 phenotypes shown after treatment with 4-HT. Individuals 1, 2, and 3 from l correspond to images labeled 1, 2, and 3.
    Figure Legend Snippet: Generation and testing of a conditional (“floxed”) tbx20 allele. a . Experimental design. b . Genotyping of adult fish for intron 2 loxP site. c . Results of nested PCR screening for loxP integration into intron 1. d . Sequence of intron 1 loxP integration in recovered tpl145 floxed tbx20 allele. e . Genotyping of “F1” adults. Primer binding sites are shown as black arrows, loxP sites as red or blue triangles, exon as an open box. f, g . Induction of tbx20 loss of function by injection of Cre mRNA. f . One quarter of embryos obtained by in-crossing tbx20 tpl145 heterozygotes and injected with Cre mRNA display a consistent, severe heart development defect. g . Genotyping of embryos with severe heart defects (lanes 1–3) and phenotypically normal siblings (lanes 4–8). L, GeneRuler DNA Ladder (ThermoFisher Scientific). Genotyping lanes 1 and 5 correspond to images in f . h, i . Induction of tbx20 deletion by 4-hydroxytamoxifen. tbx20 tpl145 heterozygote was crossed to Tg( ubi :CreERT2) line. GFP-positive embryos were collected at 2dpf and incubated with 5μM 4-HT for 24 hours. j . Adults raised from Cre-injected tbx20 tpl145/+ embryos were incrossed, resulting in approximately 1/4 of embryos (36/128, 28%) with severe heart defects. k . Confirmation of Cre-mediated excision of the second intron of tbx20 by sequence analysis. l, m . Analysis of excision efficiency by qPCR. l . Excision efficiency was assessed by qPCR in embryos treated with 4-HT at various concentrations at different time points and in two different ubb :CreERT2 driver lines. The y-axis indicates un-excised tpl 145 normalized to the untreated control. m . Images of tbx20 phenotypes shown after treatment with 4-HT. Individuals 1, 2, and 3 from l correspond to images labeled 1, 2, and 3.

    Techniques Used: Fluorescence In Situ Hybridization, Nested PCR, Sequencing, Binding Assay, Injection, Incubation, Real-time Polymerase Chain Reaction, Labeling

    2) Product Images from "Conditional mutagenesis by oligonucleotide-mediated integration of loxP sites in zebrafish"

    Article Title: Conditional mutagenesis by oligonucleotide-mediated integration of loxP sites in zebrafish

    Journal: PLoS Genetics

    doi: 10.1371/journal.pgen.1007754

    Generation and testing of a conditional (“floxed”) tbx20 allele. a . Experimental design. b . Genotyping of adult fish for intron 2 loxP site. c . Results of nested PCR screening for loxP integration into intron 1. d . Sequence of intron 1 loxP integration in recovered tpl145 floxed tbx20 allele. e . Genotyping of “F1” adults. Primer binding sites are shown as black arrows, loxP sites as red or blue triangles, exon as an open box. f, g . Induction of tbx20 loss of function by injection of Cre mRNA. f . One quarter of embryos obtained by in-crossing tbx20 tpl145 heterozygotes and injected with Cre mRNA display a consistent, severe heart development defect. g . Genotyping of embryos with severe heart defects (lanes 1–3) and phenotypically normal siblings (lanes 4–8). L, GeneRuler DNA Ladder (ThermoFisher Scientific). Genotyping lanes 1 and 5 correspond to images in f . h, i . Induction of tbx20 deletion by 4-hydroxytamoxifen. tbx20 tpl145 heterozygote was crossed to Tg( ubi :CreERT2) line. GFP-positive embryos were collected at 2dpf and incubated with 5μM 4-HT for 24 hours. j . Adults raised from Cre-injected tbx20 tpl145/+ embryos were incrossed, resulting in approximately 1/4 of embryos (36/128, 28%) with severe heart defects. k . Confirmation of Cre-mediated excision of the second intron of tbx20 by sequence analysis. l, m . Analysis of excision efficiency by qPCR. l . Excision efficiency was assessed by qPCR in embryos treated with 4-HT at various concentrations at different time points and in two different ubb :CreERT2 driver lines. The y-axis indicates un-excised tpl 145 normalized to the untreated control. m . Images of tbx20 phenotypes shown after treatment with 4-HT. Individuals 1, 2, and 3 from l correspond to images labeled 1, 2, and 3.
    Figure Legend Snippet: Generation and testing of a conditional (“floxed”) tbx20 allele. a . Experimental design. b . Genotyping of adult fish for intron 2 loxP site. c . Results of nested PCR screening for loxP integration into intron 1. d . Sequence of intron 1 loxP integration in recovered tpl145 floxed tbx20 allele. e . Genotyping of “F1” adults. Primer binding sites are shown as black arrows, loxP sites as red or blue triangles, exon as an open box. f, g . Induction of tbx20 loss of function by injection of Cre mRNA. f . One quarter of embryos obtained by in-crossing tbx20 tpl145 heterozygotes and injected with Cre mRNA display a consistent, severe heart development defect. g . Genotyping of embryos with severe heart defects (lanes 1–3) and phenotypically normal siblings (lanes 4–8). L, GeneRuler DNA Ladder (ThermoFisher Scientific). Genotyping lanes 1 and 5 correspond to images in f . h, i . Induction of tbx20 deletion by 4-hydroxytamoxifen. tbx20 tpl145 heterozygote was crossed to Tg( ubi :CreERT2) line. GFP-positive embryos were collected at 2dpf and incubated with 5μM 4-HT for 24 hours. j . Adults raised from Cre-injected tbx20 tpl145/+ embryos were incrossed, resulting in approximately 1/4 of embryos (36/128, 28%) with severe heart defects. k . Confirmation of Cre-mediated excision of the second intron of tbx20 by sequence analysis. l, m . Analysis of excision efficiency by qPCR. l . Excision efficiency was assessed by qPCR in embryos treated with 4-HT at various concentrations at different time points and in two different ubb :CreERT2 driver lines. The y-axis indicates un-excised tpl 145 normalized to the untreated control. m . Images of tbx20 phenotypes shown after treatment with 4-HT. Individuals 1, 2, and 3 from l correspond to images labeled 1, 2, and 3.

    Techniques Used: Fluorescence In Situ Hybridization, Nested PCR, Sequencing, Binding Assay, Injection, Incubation, Real-time Polymerase Chain Reaction, Labeling

    3) Product Images from "Topical 2′-Hydroxyflavanone for Cutaneous Melanoma"

    Article Title: Topical 2′-Hydroxyflavanone for Cutaneous Melanoma

    Journal: Cancers

    doi: 10.3390/cancers11101556

    ( A ) 2HF-induced apoptosis detected by DNA laddering in the B16-F0, B16-F10, and SK-MEL-24 cell lines. Mouse and human melanoma cells were incubated with or without 50 µM 2HF for 24–72 h, washed, and harvested. DNA was isolated and processed as described in Materials and Methods Section. Lane 1: Standard molecular size marker (1 Kb). Lane 2: Untreated cells, Lanes 3–5: 2HF- treated cells. ( B ) Effect of 2HF on DNA fragmentation in B16-F0, B16-F10, and SK-MEL- 24 cells as measured by terminal deoxynucleotidyl transferase dUTP-mediated nick-end labeling (TUNEL) assay. Cells were treated with different doses of 2HF for 48 h, as described in Materials and Methods. After treatments, apoptotic intensity of cells was determined by flow cytometry. Histograms show the number (counts) of TUNEL-positive cells in different groups. Data are shown as a logarithmic histogram and expressed as fluorescence intensity of number of counts of the TUNEL-positive cells obtained from the statistical analysis of the fluorescence height and mean value of the x-axis. ( C ) Effect of 2HF on RLIP and the activation of caspases. Melanoma cells were treated with 50 or 100 µM 2HF for 48 h, and then whole-cell lysates were prepared and subjected to Western blotting using antibodies against the indicated proteins.
    Figure Legend Snippet: ( A ) 2HF-induced apoptosis detected by DNA laddering in the B16-F0, B16-F10, and SK-MEL-24 cell lines. Mouse and human melanoma cells were incubated with or without 50 µM 2HF for 24–72 h, washed, and harvested. DNA was isolated and processed as described in Materials and Methods Section. Lane 1: Standard molecular size marker (1 Kb). Lane 2: Untreated cells, Lanes 3–5: 2HF- treated cells. ( B ) Effect of 2HF on DNA fragmentation in B16-F0, B16-F10, and SK-MEL- 24 cells as measured by terminal deoxynucleotidyl transferase dUTP-mediated nick-end labeling (TUNEL) assay. Cells were treated with different doses of 2HF for 48 h, as described in Materials and Methods. After treatments, apoptotic intensity of cells was determined by flow cytometry. Histograms show the number (counts) of TUNEL-positive cells in different groups. Data are shown as a logarithmic histogram and expressed as fluorescence intensity of number of counts of the TUNEL-positive cells obtained from the statistical analysis of the fluorescence height and mean value of the x-axis. ( C ) Effect of 2HF on RLIP and the activation of caspases. Melanoma cells were treated with 50 or 100 µM 2HF for 48 h, and then whole-cell lysates were prepared and subjected to Western blotting using antibodies against the indicated proteins.

    Techniques Used: DNA Laddering, Incubation, Isolation, Marker, End Labeling, TUNEL Assay, Flow Cytometry, Cytometry, Fluorescence, Activation Assay, Western Blot

    4) Product Images from "Conditional mutagenesis by oligonucleotide-mediated integration of loxP sites in zebrafish"

    Article Title: Conditional mutagenesis by oligonucleotide-mediated integration of loxP sites in zebrafish

    Journal: PLoS Genetics

    doi: 10.1371/journal.pgen.1007754

    Generation and testing of a conditional (“floxed”) tbx20 allele. a . Experimental design. b . Genotyping of adult fish for intron 2 loxP site. c . Results of nested PCR screening for loxP integration into intron 1. d . Sequence of intron 1 loxP integration in recovered tpl145 floxed tbx20 allele. e . Genotyping of “F1” adults. Primer binding sites are shown as black arrows, loxP sites as red or blue triangles, exon as an open box. f, g . Induction of tbx20 loss of function by injection of Cre mRNA. f . One quarter of embryos obtained by in-crossing tbx20 tpl145 heterozygotes and injected with Cre mRNA display a consistent, severe heart development defect. g . Genotyping of embryos with severe heart defects (lanes 1–3) and phenotypically normal siblings (lanes 4–8). L, GeneRuler DNA Ladder (ThermoFisher Scientific). Genotyping lanes 1 and 5 correspond to images in f . h, i . Induction of tbx20 deletion by 4-hydroxytamoxifen. tbx20 tpl145 heterozygote was crossed to Tg( ubi :CreERT2) line. GFP-positive embryos were collected at 2dpf and incubated with 5μM 4-HT for 24 hours. j . Adults raised from Cre-injected tbx20 tpl145/+ embryos were incrossed, resulting in approximately 1/4 of embryos (36/128, 28%) with severe heart defects. k . Confirmation of Cre-mediated excision of the second intron of tbx20 by sequence analysis. l, m . Analysis of excision efficiency by qPCR. l . Excision efficiency was assessed by qPCR in embryos treated with 4-HT at various concentrations at different time points and in two different ubb :CreERT2 driver lines. The y-axis indicates un-excised tpl 145 normalized to the untreated control. m . Images of tbx20 phenotypes shown after treatment with 4-HT. Individuals 1, 2, and 3 from l correspond to images labeled 1, 2, and 3.
    Figure Legend Snippet: Generation and testing of a conditional (“floxed”) tbx20 allele. a . Experimental design. b . Genotyping of adult fish for intron 2 loxP site. c . Results of nested PCR screening for loxP integration into intron 1. d . Sequence of intron 1 loxP integration in recovered tpl145 floxed tbx20 allele. e . Genotyping of “F1” adults. Primer binding sites are shown as black arrows, loxP sites as red or blue triangles, exon as an open box. f, g . Induction of tbx20 loss of function by injection of Cre mRNA. f . One quarter of embryos obtained by in-crossing tbx20 tpl145 heterozygotes and injected with Cre mRNA display a consistent, severe heart development defect. g . Genotyping of embryos with severe heart defects (lanes 1–3) and phenotypically normal siblings (lanes 4–8). L, GeneRuler DNA Ladder (ThermoFisher Scientific). Genotyping lanes 1 and 5 correspond to images in f . h, i . Induction of tbx20 deletion by 4-hydroxytamoxifen. tbx20 tpl145 heterozygote was crossed to Tg( ubi :CreERT2) line. GFP-positive embryos were collected at 2dpf and incubated with 5μM 4-HT for 24 hours. j . Adults raised from Cre-injected tbx20 tpl145/+ embryos were incrossed, resulting in approximately 1/4 of embryos (36/128, 28%) with severe heart defects. k . Confirmation of Cre-mediated excision of the second intron of tbx20 by sequence analysis. l, m . Analysis of excision efficiency by qPCR. l . Excision efficiency was assessed by qPCR in embryos treated with 4-HT at various concentrations at different time points and in two different ubb :CreERT2 driver lines. The y-axis indicates un-excised tpl 145 normalized to the untreated control. m . Images of tbx20 phenotypes shown after treatment with 4-HT. Individuals 1, 2, and 3 from l correspond to images labeled 1, 2, and 3.

    Techniques Used: Fluorescence In Situ Hybridization, Nested PCR, Sequencing, Binding Assay, Injection, Incubation, Real-time Polymerase Chain Reaction, Labeling

    5) Product Images from "Conditional mutagenesis by oligonucleotide-mediated integration of loxP sites in zebrafish"

    Article Title: Conditional mutagenesis by oligonucleotide-mediated integration of loxP sites in zebrafish

    Journal: PLoS Genetics

    doi: 10.1371/journal.pgen.1007754

    Generation and testing of a conditional (“floxed”) tbx20 allele. a . Experimental design. b . Genotyping of adult fish for intron 2 loxP site. c . Results of nested PCR screening for loxP integration into intron 1. d . Sequence of intron 1 loxP integration in recovered tpl145 floxed tbx20 allele. e . Genotyping of “F1” adults. Primer binding sites are shown as black arrows, loxP sites as red or blue triangles, exon as an open box. f, g . Induction of tbx20 loss of function by injection of Cre mRNA. f . One quarter of embryos obtained by in-crossing tbx20 tpl145 heterozygotes and injected with Cre mRNA display a consistent, severe heart development defect. g . Genotyping of embryos with severe heart defects (lanes 1–3) and phenotypically normal siblings (lanes 4–8). L, GeneRuler DNA Ladder (ThermoFisher Scientific). Genotyping lanes 1 and 5 correspond to images in f . h, i . Induction of tbx20 deletion by 4-hydroxytamoxifen. tbx20 tpl145 heterozygote was crossed to Tg( ubi :CreERT2) line. GFP-positive embryos were collected at 2dpf and incubated with 5μM 4-HT for 24 hours. j . Adults raised from Cre-injected tbx20 tpl145/+ embryos were incrossed, resulting in approximately 1/4 of embryos (36/128, 28%) with severe heart defects. k . Confirmation of Cre-mediated excision of the second intron of tbx20 by sequence analysis. l, m . Analysis of excision efficiency by qPCR. l . Excision efficiency was assessed by qPCR in embryos treated with 4-HT at various concentrations at different time points and in two different ubb :CreERT2 driver lines. The y-axis indicates un-excised tpl 145 normalized to the untreated control. m . Images of tbx20 phenotypes shown after treatment with 4-HT. Individuals 1, 2, and 3 from l correspond to images labeled 1, 2, and 3.
    Figure Legend Snippet: Generation and testing of a conditional (“floxed”) tbx20 allele. a . Experimental design. b . Genotyping of adult fish for intron 2 loxP site. c . Results of nested PCR screening for loxP integration into intron 1. d . Sequence of intron 1 loxP integration in recovered tpl145 floxed tbx20 allele. e . Genotyping of “F1” adults. Primer binding sites are shown as black arrows, loxP sites as red or blue triangles, exon as an open box. f, g . Induction of tbx20 loss of function by injection of Cre mRNA. f . One quarter of embryos obtained by in-crossing tbx20 tpl145 heterozygotes and injected with Cre mRNA display a consistent, severe heart development defect. g . Genotyping of embryos with severe heart defects (lanes 1–3) and phenotypically normal siblings (lanes 4–8). L, GeneRuler DNA Ladder (ThermoFisher Scientific). Genotyping lanes 1 and 5 correspond to images in f . h, i . Induction of tbx20 deletion by 4-hydroxytamoxifen. tbx20 tpl145 heterozygote was crossed to Tg( ubi :CreERT2) line. GFP-positive embryos were collected at 2dpf and incubated with 5μM 4-HT for 24 hours. j . Adults raised from Cre-injected tbx20 tpl145/+ embryos were incrossed, resulting in approximately 1/4 of embryos (36/128, 28%) with severe heart defects. k . Confirmation of Cre-mediated excision of the second intron of tbx20 by sequence analysis. l, m . Analysis of excision efficiency by qPCR. l . Excision efficiency was assessed by qPCR in embryos treated with 4-HT at various concentrations at different time points and in two different ubb :CreERT2 driver lines. The y-axis indicates un-excised tpl 145 normalized to the untreated control. m . Images of tbx20 phenotypes shown after treatment with 4-HT. Individuals 1, 2, and 3 from l correspond to images labeled 1, 2, and 3.

    Techniques Used: Fluorescence In Situ Hybridization, Nested PCR, Sequencing, Binding Assay, Injection, Incubation, Real-time Polymerase Chain Reaction, Labeling

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    Protease Inhibitor:

    Article Title: An efficient method of directly cloning chimpanzee adenovirus as a vaccine vector
    Article Snippet: EcoRI (New England Biolabs, cat. no. R0101S) KpnI (New England Biolabs, cat. no. R0142S) PmeI (New England Biolabs, cat. no. R0560S) PacI (New England Biolabs, cat. no. R0547S) XbaI (New England Biolabs, cat. no. R0145S) SnaBI (New England Biolabs, cat. no. R0130S) NdeI (New England Biolabs, cat. no. R0111S) SphI (New England Biolabs, cat. no. R0182S) HindIII (New England Biolabs, cat. no. R0104S) AgeI (New England Biolabs, cat. no. R0552S) MluI (New England Biolabs, cat. no. R0198S) SbfI (New England Biolabs, cat. no. R0642S) BstAPI (New England Biolabs, cat. no. V0269S) SwaI (New England Biolabs, cat. no. R0604S) I-CeuI (New England Biolabs, cat. no. R0699S) PI-SceI (New England Biolabs, cat. no. R0696S) SgfI (Promega, cat. no. R5104) Eco47III (Promega, cat. no. R6731) Phusion hot start high-fidelity DNA polymerase (England Biolabs, cat. no. F-540S) dNTP mix (10 mM each; Invitrogen, cat. no. R72501) Alkaline phosphatase (AP; Roche, cat. no. 13826120) T4 DNA ligase (Roche, cat. no. 13827621) DNA ladders (Invitrogen, cat. nos. .. 10381-010 and 15628-019) Agarose (low melting point; Sigma-Aldrich, cat. no. A9414-25G) UltraPure agarose (Invitrogen, cat. no. 15510-027) l -broth (LB) capsules (MP Biomedicals, cat. no. 3001-031) Ampicillin (Mediatech, cat. no. 61-238-RH) Kanamycin (Roche, cat. no. 106801) Pronase (Boehringer Mannheim, cat. no. 165921) Proteinase K (Boehringer Mannheim, cat. no. 745723) Complete protease inhibitor cocktail tablets (Roche, cat. no. 04693124001) SDS solution (10% (wt/vol); Ambion, cat. no. 9822) Tris-HCl (1 M, pH 8.0; Sigma-Aldrich, cat. no. T-2694) Plasmid DNA mini and midi preparation kit (Qiagen) pShuttle (Clontech, cat. no. K1650-1) pNEB193 (New England Biolabs, cat. no. N3051S) Lipofectamine 2000 transfection reagent (Invitrogen, cat. no. 11668-027) DMEM (Mediatech, cat. no. 10-017-CV) Leibovitz’s L-15 medium (L-15; Mediatech, cat. no. 10-045-CV) FBS (PAA Laboratories, cat. no. A11-034) Penicillin-streptomycin (Mediatech, cat. no. 30-002-CI) Trypsin-EDTA (Mediatech, cat. no. 25-053-CI) Primers and oligo linker (Integrated DNA Technologies) Codon-optimized HIV gag (GenScript) DNeasy extraction kit (Qiagen) Distilled water

    Article Title: WHEP Domains Direct Noncanonical Function of Glutamyl-Prolyl tRNA Synthetase in Translational Control of Gene Expression
    Article Snippet: Protein purification reagents, immunoanalysis reagents, protease inhibitor and protein-stabilizing cocktails, phosphatase inhibitors, protein cross-linking and staining reagents were from Pierce (Rockford, IL). .. Protein and DNA ladders were from Fermentas (Hanover, MD).

    Footprinting:

    Article Title: Mechanistic studies of DepR in regulating FK228 biosynthesis in Chromobacterium violaceum no. 968
    Article Snippet: Paragraph title: DNase I footprinting assay (DIFA) ... The preparation of DNA ladders, electrophoresis and data analysis were the same as described [ ], except that the GeneScan-LIZ500 size standard (Applied BioSystems) was used.

    DNA Laddering:

    Article Title: Topical 2′-Hydroxyflavanone for Cutaneous Melanoma
    Article Snippet: 2HF-Induced Apoptosis in Melanoma Cell Lines in Vitro The DNA laddering assay was used to examine whether cell death caused by 2HF was accompanied by apoptosis. .. 2HF-induced apoptosis was confirmed by the appearance of DNA ladders in SYBR-Safe (Invitrogen)-stained agarose gels of nuclear extracts of B16-F0, B16-F10, and SK-MEL-24 cells treated with 50 μM 2HF.

    Transmission Assay:

    Article Title: Conditional mutagenesis by oligonucleotide-mediated integration of loxP sites in zebrafish
    Article Snippet: Paragraph title: Screening for germline transmission of loxP integrations by nested PCR ... DNA ladders used were Thermo Scientific GeneRuler DNA ladder (SM0331) and ThermoFisher Scientific 100 bp DNA ladder (#SM0241)( ).

    Polymerase Chain Reaction:

    Article Title: Validation of SYBR Green based quantification assay for the detection of human Torque Teno virus titers from plasma
    Article Snippet: All the experiments were performed on StepOnePlus®-Real Time PCR Systems by Applied Biosystems, Fostercity, CA. .. DNA ladders, MgCl2 , dNTP’s and buffers were obtained from Fermentas Life sciences, Germany.

    Article Title: Conditional mutagenesis by oligonucleotide-mediated integration of loxP sites in zebrafish
    Article Snippet: All PCR reactions were performed using either NEB 2X Taq Master Mix, Thermo Scientific Taq or Kapa 2G Fast ReadyMix. .. DNA ladders used were Thermo Scientific GeneRuler DNA ladder (SM0331) and ThermoFisher Scientific 100 bp DNA ladder (#SM0241)( ).

    Article Title: Conditional mutagenesis by oligonucleotide-mediated integration of loxP sites in zebrafish
    Article Snippet: Paragraph title: Screening F1s for loxP integrations by short flanking PCR ... DNA ladders used were Thermo Scientific GeneRuler DNA ladder.

    Article Title: Development and validation of an allele-specific PCR assay for genotyping a promoter and exonic single nucleotide polymorphisms of MGMT gene
    Article Snippet: The allele-specific primer and the common primers were combined in two parallel PCR reactions (Primer sequences and commentary are listed in ). .. DNA ladders, MgCl2 , dNTP’s were obtained from Fermentas Life sciences (Germany), and Taq DNA Polymerase gold and buffer were obtained from Promega (Madison, Wisconsin, USA).

    Article Title: Mechanistic studies of DepR in regulating FK228 biosynthesis in Chromobacterium violaceum no. 968
    Article Snippet: The FAM-labeled PCR product, purified with a Wizard® SV Gel and PCR Clean-Up System (Promega, USA), was quantified with NanoDrop 2000C spectrometer (Thermo, USA). .. The preparation of DNA ladders, electrophoresis and data analysis were the same as described [ ], except that the GeneScan-LIZ500 size standard (Applied BioSystems) was used.

    Article Title: WHEP Domains Direct Noncanonical Function of Glutamyl-Prolyl tRNA Synthetase in Translational Control of Gene Expression
    Article Snippet: Rabbit reticulocyte lysate, wheat germ extract, transcend tRNA, restriction enzymes, and PCR master mix were purchased from Promega (Madison, WI). .. Protein and DNA ladders were from Fermentas (Hanover, MD).

    High Performance Liquid Chromatography:

    Article Title: Development and validation of an allele-specific PCR assay for genotyping a promoter and exonic single nucleotide polymorphisms of MGMT gene
    Article Snippet: HPLC purified allele-specific primers were synthesized by microsynth (Switzerland) at a scale of 0.2 μmol. .. DNA ladders, MgCl2 , dNTP’s were obtained from Fermentas Life sciences (Germany), and Taq DNA Polymerase gold and buffer were obtained from Promega (Madison, Wisconsin, USA).

    Nucleic Acid Electrophoresis:

    Article Title: Conditional mutagenesis by oligonucleotide-mediated integration of loxP sites in zebrafish
    Article Snippet: The first flanking PCR was run and amplification was confirmed by gel electrophoresis. .. DNA ladders used were Thermo Scientific GeneRuler DNA ladder (SM0331) and ThermoFisher Scientific 100 bp DNA ladder (#SM0241)( ).

    Fluorescence:

    Article Title: Topical 2′-Hydroxyflavanone for Cutaneous Melanoma
    Article Snippet: 2HF-induced apoptosis was confirmed by the appearance of DNA ladders in SYBR-Safe (Invitrogen)-stained agarose gels of nuclear extracts of B16-F0, B16-F10, and SK-MEL-24 cells treated with 50 μM 2HF. .. Apoptosis was confirmed by fluorescence-activated cell sorting (FACS) analysis using the APO-BrdU™ terminal deoxynucleotidyl transferase dUTP-mediated nick-end labeling (TUNEL) Assay Kit, which detects terminal deoxynucleotidyl transferase (TdT)-mediated nick-end labeling.

    Labeling:

    Article Title: Topical 2′-Hydroxyflavanone for Cutaneous Melanoma
    Article Snippet: 2HF-induced apoptosis was confirmed by the appearance of DNA ladders in SYBR-Safe (Invitrogen)-stained agarose gels of nuclear extracts of B16-F0, B16-F10, and SK-MEL-24 cells treated with 50 μM 2HF. .. Apoptosis was confirmed by fluorescence-activated cell sorting (FACS) analysis using the APO-BrdU™ terminal deoxynucleotidyl transferase dUTP-mediated nick-end labeling (TUNEL) Assay Kit, which detects terminal deoxynucleotidyl transferase (TdT)-mediated nick-end labeling.

    Article Title: Mechanistic studies of DepR in regulating FK228 biosynthesis in Chromobacterium violaceum no. 968
    Article Snippet: For preparation of fluorescent FAM labeled probes, the promoter region of orf21 was amplified with primer pair M13F/M13R-48 (FAM) from pYJ01 using Dpx DNA polymerase (TOLO Biotech, Shanghai, China). .. The preparation of DNA ladders, electrophoresis and data analysis were the same as described [ ], except that the GeneScan-LIZ500 size standard (Applied BioSystems) was used.

    Purification:

    Article Title: Conditional mutagenesis by oligonucleotide-mediated integration of loxP sites in zebrafish
    Article Snippet: PCRs were run on 2% agarose gels, and amplicons of appropriate size were purified and sequenced to confirm loxP integration. .. DNA ladders used were Thermo Scientific GeneRuler DNA ladder.

    Article Title: miR-320a mediates doxorubicin-induced cardiotoxicity by targeting VEGF signal pathway
    Article Snippet: Endotoxin-free plasmid purification kits were from TIANGEN (Beijing, China). .. DNA ladders, prestained protein markers were from Thermo Fisher Scientific (Glen Buenie, MD).

    Article Title: Development and validation of an allele-specific PCR assay for genotyping a promoter and exonic single nucleotide polymorphisms of MGMT gene
    Article Snippet: HPLC purified allele-specific primers were synthesized by microsynth (Switzerland) at a scale of 0.2 μmol. .. DNA ladders, MgCl2 , dNTP’s were obtained from Fermentas Life sciences (Germany), and Taq DNA Polymerase gold and buffer were obtained from Promega (Madison, Wisconsin, USA).

    Article Title: Mechanistic studies of DepR in regulating FK228 biosynthesis in Chromobacterium violaceum no. 968
    Article Snippet: The FAM-labeled PCR product, purified with a Wizard® SV Gel and PCR Clean-Up System (Promega, USA), was quantified with NanoDrop 2000C spectrometer (Thermo, USA). .. The preparation of DNA ladders, electrophoresis and data analysis were the same as described [ ], except that the GeneScan-LIZ500 size standard (Applied BioSystems) was used.

    Article Title: Gene Deletion of 7,8-Linoleate Diol Synthase of the Rice Blast Fungus
    Article Snippet: 18:2 n -6 (99%), 18:3 n -6 (99%), and 18:3 n -3 (99%) were from VWR, all other fatty acids (98–99%) were from Larodan (Malmö, Sweden). (7 R )-[2 H]18:2 n -6, (8 R )-[2 H]18:2 n -6, and (11 S )-[2 H]18:2 n -6 were prepared as described previously ( , ). (13 R )-[2 H4 ]HODE, (8 R )-HPODE, and stereoisomers of 8,11-DiHODE were prepared and purified as described previously ( ). .. Chemically competent Escherichia coli (Top10), DNA ladders, reverse transcriptase (Superscript III), and pCR2.1 were from Invitrogen. pGEM5Zf+ and DNase I were obtained from Promega, and pCAMBIA0380 was from CAMBIA (Canberra, Australia).

    Protein Purification:

    Article Title: WHEP Domains Direct Noncanonical Function of Glutamyl-Prolyl tRNA Synthetase in Translational Control of Gene Expression
    Article Snippet: Protein purification reagents, immunoanalysis reagents, protease inhibitor and protein-stabilizing cocktails, phosphatase inhibitors, protein cross-linking and staining reagents were from Pierce (Rockford, IL). .. Protein and DNA ladders were from Fermentas (Hanover, MD).

    FACS:

    Article Title: Topical 2′-Hydroxyflavanone for Cutaneous Melanoma
    Article Snippet: 2HF-induced apoptosis was confirmed by the appearance of DNA ladders in SYBR-Safe (Invitrogen)-stained agarose gels of nuclear extracts of B16-F0, B16-F10, and SK-MEL-24 cells treated with 50 μM 2HF. .. Apoptosis was confirmed by fluorescence-activated cell sorting (FACS) analysis using the APO-BrdU™ terminal deoxynucleotidyl transferase dUTP-mediated nick-end labeling (TUNEL) Assay Kit, which detects terminal deoxynucleotidyl transferase (TdT)-mediated nick-end labeling.

    Nested PCR:

    Article Title: Conditional mutagenesis by oligonucleotide-mediated integration of loxP sites in zebrafish
    Article Snippet: Paragraph title: Screening for germline transmission of loxP integrations by nested PCR ... DNA ladders used were Thermo Scientific GeneRuler DNA ladder (SM0331) and ThermoFisher Scientific 100 bp DNA ladder (#SM0241)( ).

    SPR Assay:

    Article Title: WHEP Domains Direct Noncanonical Function of Glutamyl-Prolyl tRNA Synthetase in Translational Control of Gene Expression
    Article Snippet: Protein and DNA ladders were from Fermentas (Hanover, MD). .. SPR chips and buffers were from Biacore (Piscataway, NJ).

    Plasmid Preparation:

    Article Title: miR-320a mediates doxorubicin-induced cardiotoxicity by targeting VEGF signal pathway
    Article Snippet: Endotoxin-free plasmid purification kits were from TIANGEN (Beijing, China). .. DNA ladders, prestained protein markers were from Thermo Fisher Scientific (Glen Buenie, MD).

    Article Title: An efficient method of directly cloning chimpanzee adenovirus as a vaccine vector
    Article Snippet: EcoRI (New England Biolabs, cat. no. R0101S) KpnI (New England Biolabs, cat. no. R0142S) PmeI (New England Biolabs, cat. no. R0560S) PacI (New England Biolabs, cat. no. R0547S) XbaI (New England Biolabs, cat. no. R0145S) SnaBI (New England Biolabs, cat. no. R0130S) NdeI (New England Biolabs, cat. no. R0111S) SphI (New England Biolabs, cat. no. R0182S) HindIII (New England Biolabs, cat. no. R0104S) AgeI (New England Biolabs, cat. no. R0552S) MluI (New England Biolabs, cat. no. R0198S) SbfI (New England Biolabs, cat. no. R0642S) BstAPI (New England Biolabs, cat. no. V0269S) SwaI (New England Biolabs, cat. no. R0604S) I-CeuI (New England Biolabs, cat. no. R0699S) PI-SceI (New England Biolabs, cat. no. R0696S) SgfI (Promega, cat. no. R5104) Eco47III (Promega, cat. no. R6731) Phusion hot start high-fidelity DNA polymerase (England Biolabs, cat. no. F-540S) dNTP mix (10 mM each; Invitrogen, cat. no. R72501) Alkaline phosphatase (AP; Roche, cat. no. 13826120) T4 DNA ligase (Roche, cat. no. 13827621) DNA ladders (Invitrogen, cat. nos. .. 10381-010 and 15628-019) Agarose (low melting point; Sigma-Aldrich, cat. no. A9414-25G) UltraPure agarose (Invitrogen, cat. no. 15510-027) l -broth (LB) capsules (MP Biomedicals, cat. no. 3001-031) Ampicillin (Mediatech, cat. no. 61-238-RH) Kanamycin (Roche, cat. no. 106801) Pronase (Boehringer Mannheim, cat. no. 165921) Proteinase K (Boehringer Mannheim, cat. no. 745723) Complete protease inhibitor cocktail tablets (Roche, cat. no. 04693124001) SDS solution (10% (wt/vol); Ambion, cat. no. 9822) Tris-HCl (1 M, pH 8.0; Sigma-Aldrich, cat. no. T-2694) Plasmid DNA mini and midi preparation kit (Qiagen) pShuttle (Clontech, cat. no. K1650-1) pNEB193 (New England Biolabs, cat. no. N3051S) Lipofectamine 2000 transfection reagent (Invitrogen, cat. no. 11668-027) DMEM (Mediatech, cat. no. 10-017-CV) Leibovitz’s L-15 medium (L-15; Mediatech, cat. no. 10-045-CV) FBS (PAA Laboratories, cat. no. A11-034) Penicillin-streptomycin (Mediatech, cat. no. 30-002-CI) Trypsin-EDTA (Mediatech, cat. no. 25-053-CI) Primers and oligo linker (Integrated DNA Technologies) Codon-optimized HIV gag (GenScript) DNeasy extraction kit (Qiagen) Distilled water

    Real-time Polymerase Chain Reaction:

    Article Title: Validation of SYBR Green based quantification assay for the detection of human Torque Teno virus titers from plasma
    Article Snippet: SYBR®-Green PCR master mix, 96 well MicroAmp® fast optical reaction plates (0.1 mL capacity) and MicroAmp® optical adhesive films for real-time PCR assay were obtained from Applied Biosystems, Fostercity, CA. .. DNA ladders, MgCl2 , dNTP’s and buffers were obtained from Fermentas Life sciences, Germany.

    Negative Control:

    Article Title: miR-320a mediates doxorubicin-induced cardiotoxicity by targeting VEGF signal pathway
    Article Snippet: Horseradish peroxidase-conjugated secondary antibody and enhanced chemiluminescence reagents were from Pierce Biotech (Rockford, IL). miR-320a mimics/inhibitors, VEGF-A siRNAs, negative control and primers for miRNAs were from RiboBio (Guangzhou, China). .. DNA ladders, prestained protein markers were from Thermo Fisher Scientific (Glen Buenie, MD).

    In Vitro:

    Article Title: Topical 2′-Hydroxyflavanone for Cutaneous Melanoma
    Article Snippet: Paragraph title: 2.2. 2HF-Induced Apoptosis in Melanoma Cell Lines in Vitro ... 2HF-induced apoptosis was confirmed by the appearance of DNA ladders in SYBR-Safe (Invitrogen)-stained agarose gels of nuclear extracts of B16-F0, B16-F10, and SK-MEL-24 cells treated with 50 μM 2HF.

    Electrophoresis:

    Article Title: Mechanistic studies of DepR in regulating FK228 biosynthesis in Chromobacterium violaceum no. 968
    Article Snippet: .. The preparation of DNA ladders, electrophoresis and data analysis were the same as described [ ], except that the GeneScan-LIZ500 size standard (Applied BioSystems) was used. .. Determination of promoter activity by GFP production The promoterless gfp gene from pWHU1421 (provided by Dr. L. Cheng, Wuhan University, unpublished) was used as reporter gene. gfp was amplified with primer pair DQ132F/DQ132R and cloned into the downstream NdeI/BglII sites of T7 promoter of pACYCDuet-1 to generate pWHU3026.

    Incubation:

    Article Title: Mechanistic studies of DepR in regulating FK228 biosynthesis in Chromobacterium violaceum no. 968
    Article Snippet: For each assay, 400 ng of DNA probe was incubated with different amounts of native DepR or mutated DepR(C199S) protein in a total volume of 40 μL at 25 °C for 30 min. A 10 μL solution containing 0.015 unit of DNase I (Promega, USA) and 100 nmol of freshly prepared CaCl2 was added to the reaction and further incubated at 25 °C for 1 min. .. The preparation of DNA ladders, electrophoresis and data analysis were the same as described [ ], except that the GeneScan-LIZ500 size standard (Applied BioSystems) was used.

    Staining:

    Article Title: Topical 2′-Hydroxyflavanone for Cutaneous Melanoma
    Article Snippet: 2HF-induced apoptosis was confirmed by the appearance of DNA ladders in SYBR-Safe (Invitrogen)-stained agarose gels of nuclear extracts of B16-F0, B16-F10, and SK-MEL-24 cells treated with 50 μM 2HF. .. The intensity of staining increased in a time-dependent manner at 24, 48, and 72 h ( A).

    Article Title: WHEP Domains Direct Noncanonical Function of Glutamyl-Prolyl tRNA Synthetase in Translational Control of Gene Expression
    Article Snippet: Protein purification reagents, immunoanalysis reagents, protease inhibitor and protein-stabilizing cocktails, phosphatase inhibitors, protein cross-linking and staining reagents were from Pierce (Rockford, IL). .. Protein and DNA ladders were from Fermentas (Hanover, MD).

    Variant Assay:

    Article Title: Development and validation of an allele-specific PCR assay for genotyping a promoter and exonic single nucleotide polymorphisms of MGMT gene
    Article Snippet: For variant (c.-459C > A) two forward primers with a mismatch in the last 3' nucleotides were designed along with a common reverse primer downstream of the polymorphic site with no mismatches. .. DNA ladders, MgCl2 , dNTP’s were obtained from Fermentas Life sciences (Germany), and Taq DNA Polymerase gold and buffer were obtained from Promega (Madison, Wisconsin, USA).

    Fluorescence In Situ Hybridization:

    Article Title: Conditional mutagenesis by oligonucleotide-mediated integration of loxP sites in zebrafish
    Article Snippet: Adult F1 fish were tail clipped, DNA prepped, and genotyped by running a short flanking PCR (gene specific primers described below) designed to amplify approximately 450 bp or less. .. DNA ladders used were Thermo Scientific GeneRuler DNA ladder.

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  • 90
    Thermo Fisher generuler dna ladder
    Investigation of the horizontal transmission of T . b . gambiense 1135 (Rluc) by crossing healthy female (n = 8) mice with infected male mice (n = 5). BLI signal from ex vivo organs of female mice (n = 8) crossed with T . b . gambiense 1135 infected males (n = 5) examined 7 months after crossing. A. BLI of ex vivo organs of a representative female mouse (mouse 229, see S3 Table ). Ov, ovaries; Ut, uterus; B, brain; SC, spinal cord; Sp, spleen; Li, liver; Lu, lungs; K, kidneys; In, intestines; H, heart. The colour scale to the right of the image indicates the colour intensity in ph/sr/cm 2 /s. B. Percentage of positive female mice per organ. C. 1.8% agarose gel of PCR using pMUTec/TBingi nested primers on organs of female mice 229 and 228. Lane 1: spinal cord negative control; lane 2: spleen negative control; lane 3: ovary mouse 229; lane 4: uterus mouse 229 (band 201 bp); lane 5: spinal cord negative control; lane 6 spinal cord mouse 229; lane 7: spleen mouse 229; lane 8: ovary mouse 228; lane 9: uterus mouse 228; lane 10: spinal cord mouse 228; lane 11: genomic <t>DNA</t> T . b . brucei (nested PCR of the first PCR positive control); lane 12: genomic DNA T . b . brucei (nested PCR positive control); lane M: <t>GeneRuler</t> DNA ladder (ThermoScientific).
    Generuler Dna Ladder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/generuler dna ladder/product/Thermo Fisher
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    generuler dna ladder - by Bioz Stars, 2020-02
    90/100 stars
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    90
    Thermo Fisher bp ladder dna size marker
    <t>PRA</t> patterns for different mycobacterial species. (A) Cfr13I digests; (B) BstHHI digests. <t>M-DNA</t> marker; 1- Mycobacterium scrofulaceum ; 2- Mycobacterium avium ; 3- Mycobacterium simiae ; 4- Mycobacterium gastri ; 5- Mycobacterium chitae ; 6- Mycobacterium xenopi
    Bp Ladder Dna Size Marker, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bp ladder dna size marker/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bp ladder dna size marker - by Bioz Stars, 2020-02
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    90
    Thermo Fisher generuler 1kb dna ladder plus
    RAPD profiles. RAPD profiles generated by (A) P4 primer and (B) OPA2 primer for rhizosphere (VV/R1 to VV/R5) and endophytic (VV/E1 to VV/E5) Streptomyces sp. strains isolated from the root system of young grapevine plants. MW: <t>GeneRuler</t> <t>1kb</t> <t>DNA</t> Ladder Plus (Thermo Fisher Scientific). Specific bands amplified from isolates VV/E1 and VV/R4 that were selected for SCAR markers design are indicated by arrows. NC (negative control).
    Generuler 1kb Dna Ladder Plus, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/generuler 1kb dna ladder plus/product/Thermo Fisher
    Average 90 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    generuler 1kb dna ladder plus - by Bioz Stars, 2020-02
    90/100 stars
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    Image Search Results


    Investigation of the horizontal transmission of T . b . gambiense 1135 (Rluc) by crossing healthy female (n = 8) mice with infected male mice (n = 5). BLI signal from ex vivo organs of female mice (n = 8) crossed with T . b . gambiense 1135 infected males (n = 5) examined 7 months after crossing. A. BLI of ex vivo organs of a representative female mouse (mouse 229, see S3 Table ). Ov, ovaries; Ut, uterus; B, brain; SC, spinal cord; Sp, spleen; Li, liver; Lu, lungs; K, kidneys; In, intestines; H, heart. The colour scale to the right of the image indicates the colour intensity in ph/sr/cm 2 /s. B. Percentage of positive female mice per organ. C. 1.8% agarose gel of PCR using pMUTec/TBingi nested primers on organs of female mice 229 and 228. Lane 1: spinal cord negative control; lane 2: spleen negative control; lane 3: ovary mouse 229; lane 4: uterus mouse 229 (band 201 bp); lane 5: spinal cord negative control; lane 6 spinal cord mouse 229; lane 7: spleen mouse 229; lane 8: ovary mouse 228; lane 9: uterus mouse 228; lane 10: spinal cord mouse 228; lane 11: genomic DNA T . b . brucei (nested PCR of the first PCR positive control); lane 12: genomic DNA T . b . brucei (nested PCR positive control); lane M: GeneRuler DNA ladder (ThermoScientific).

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Trypanosoma brucei gambiense Infections in Mice Lead to Tropism to the Reproductive Organs, and Horizontal and Vertical Transmission

    doi: 10.1371/journal.pntd.0004350

    Figure Lengend Snippet: Investigation of the horizontal transmission of T . b . gambiense 1135 (Rluc) by crossing healthy female (n = 8) mice with infected male mice (n = 5). BLI signal from ex vivo organs of female mice (n = 8) crossed with T . b . gambiense 1135 infected males (n = 5) examined 7 months after crossing. A. BLI of ex vivo organs of a representative female mouse (mouse 229, see S3 Table ). Ov, ovaries; Ut, uterus; B, brain; SC, spinal cord; Sp, spleen; Li, liver; Lu, lungs; K, kidneys; In, intestines; H, heart. The colour scale to the right of the image indicates the colour intensity in ph/sr/cm 2 /s. B. Percentage of positive female mice per organ. C. 1.8% agarose gel of PCR using pMUTec/TBingi nested primers on organs of female mice 229 and 228. Lane 1: spinal cord negative control; lane 2: spleen negative control; lane 3: ovary mouse 229; lane 4: uterus mouse 229 (band 201 bp); lane 5: spinal cord negative control; lane 6 spinal cord mouse 229; lane 7: spleen mouse 229; lane 8: ovary mouse 228; lane 9: uterus mouse 228; lane 10: spinal cord mouse 228; lane 11: genomic DNA T . b . brucei (nested PCR of the first PCR positive control); lane 12: genomic DNA T . b . brucei (nested PCR positive control); lane M: GeneRuler DNA ladder (ThermoScientific).

    Article Snippet: Lane M: GeneRuler DNA ladder (Thermo Scientific). (DOCX) Click here for additional data file.

    Techniques: Transmission Assay, Mouse Assay, Infection, Ex Vivo, Agarose Gel Electrophoresis, Polymerase Chain Reaction, Negative Control, Nested PCR, Positive Control

    PRA patterns for different mycobacterial species. (A) Cfr13I digests; (B) BstHHI digests. M-DNA marker; 1- Mycobacterium scrofulaceum ; 2- Mycobacterium avium ; 3- Mycobacterium simiae ; 4- Mycobacterium gastri ; 5- Mycobacterium chitae ; 6- Mycobacterium xenopi

    Journal: Saudi Journal of Biological Sciences

    Article Title: Distribution of non-tuberculosis mycobacteria strains from suspected tuberculosis patients by heat shock protein 65 PCR–RFLP

    doi: 10.1016/j.sjbs.2016.02.001

    Figure Lengend Snippet: PRA patterns for different mycobacterial species. (A) Cfr13I digests; (B) BstHHI digests. M-DNA marker; 1- Mycobacterium scrofulaceum ; 2- Mycobacterium avium ; 3- Mycobacterium simiae ; 4- Mycobacterium gastri ; 5- Mycobacterium chitae ; 6- Mycobacterium xenopi

    Article Snippet: The digested products were visualized on 3% agarose gel electrophoresis at 100 V for 3 h. To interpret the PRA profiles generated by each species, a 50 bp ladder DNA size marker (Thermo Scientific™, USA) was applied.

    Techniques: Marker

    RAPD profiles. RAPD profiles generated by (A) P4 primer and (B) OPA2 primer for rhizosphere (VV/R1 to VV/R5) and endophytic (VV/E1 to VV/E5) Streptomyces sp. strains isolated from the root system of young grapevine plants. MW: GeneRuler 1kb DNA Ladder Plus (Thermo Fisher Scientific). Specific bands amplified from isolates VV/E1 and VV/R4 that were selected for SCAR markers design are indicated by arrows. NC (negative control).

    Journal: PLoS ONE

    Article Title: Developing tools for evaluating inoculation methods of biocontrol Streptomyces sp. strains into grapevine plants

    doi: 10.1371/journal.pone.0211225

    Figure Lengend Snippet: RAPD profiles. RAPD profiles generated by (A) P4 primer and (B) OPA2 primer for rhizosphere (VV/R1 to VV/R5) and endophytic (VV/E1 to VV/E5) Streptomyces sp. strains isolated from the root system of young grapevine plants. MW: GeneRuler 1kb DNA Ladder Plus (Thermo Fisher Scientific). Specific bands amplified from isolates VV/E1 and VV/R4 that were selected for SCAR markers design are indicated by arrows. NC (negative control).

    Article Snippet: MW: GeneRuler 1kb DNA Ladder Plus (Thermo Fisher Scientific).

    Techniques: Generated, Isolation, Amplification, Negative Control

    TthHB27I cofactor/analogue-induced ‘star’ activity towards methylated or non-methylated substrate DNA. 0.5 μg of methylated or non-methylated PCR DNA fragment (see Additional file 1 ) was digested with 2 U of TthHB27I in REase buffer supplemented with 100 μM of the selected effector and 25% (v/v) DMSO at 65 °C. The pictures on the right side of the figure’s panels show the theoretical arrangement of the DNA bands in an agarose gel after digesting the given substrate DNA with TthHB27I under standard conditions. a Cleavage pattern of non-methylated 1789 bp PCR fragment DNA. Lane M1, GeneRuler 1 kb DNA Ladder; lane M2, 100 bp Plus DNA Ladder, lane K, untreated DNA; lane 1, cleavage reaction in the absence of effector and DMSO; lane 2, in the presence of DMSO only; lane 3, in the presence of SAM only; lane 4, in the presence of SAM and DMSO; lane 5, in the presence of SIN only; lane 6, in the presence of SIN and DMSO. b The same as ( a ), but with previously methylated 1789 bp PCR fragment. c The same as ( a ), but with 1850 bp PCR fragment without TthHB27I cognate recognition sequence

    Journal: BMC Genomics

    Article Title: Randomized DNA libraries construction tool: a new 3-bp ‘frequent cutter’ TthHB27I/sinefungin endonuclease with chemically-induced specificity

    doi: 10.1186/s12864-018-4748-0

    Figure Lengend Snippet: TthHB27I cofactor/analogue-induced ‘star’ activity towards methylated or non-methylated substrate DNA. 0.5 μg of methylated or non-methylated PCR DNA fragment (see Additional file 1 ) was digested with 2 U of TthHB27I in REase buffer supplemented with 100 μM of the selected effector and 25% (v/v) DMSO at 65 °C. The pictures on the right side of the figure’s panels show the theoretical arrangement of the DNA bands in an agarose gel after digesting the given substrate DNA with TthHB27I under standard conditions. a Cleavage pattern of non-methylated 1789 bp PCR fragment DNA. Lane M1, GeneRuler 1 kb DNA Ladder; lane M2, 100 bp Plus DNA Ladder, lane K, untreated DNA; lane 1, cleavage reaction in the absence of effector and DMSO; lane 2, in the presence of DMSO only; lane 3, in the presence of SAM only; lane 4, in the presence of SAM and DMSO; lane 5, in the presence of SIN only; lane 6, in the presence of SIN and DMSO. b The same as ( a ), but with previously methylated 1789 bp PCR fragment. c The same as ( a ), but with 1850 bp PCR fragment without TthHB27I cognate recognition sequence

    Article Snippet: DNA isolation kit (GeneJet Plasmid Miniprep Kit), DNA markers (GeneRuler 1 kb DNA Ladder, 100 bp Plus DNA Ladder), SmaI REase, FastAP Thermosensitive Alkaline Phosphatase, T4 DNA Polymerase and T4 DNA Ligase were from Thermo Fisher Scientific/Fermentas (Vilnius, USA/Lithuania).

    Techniques: Activity Assay, Methylation, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Sequencing

    Cleavage of λ DNA under TthHB27I specificity relaxation conditions. a Cleavage pattern of λ DNA digested with TthHB27I under various conditions. 0.5 μg of DNA substrate was digested with 2 U of TthHB27I in REase buffer supplemented with 100 μM SAM and/or 25% (v/v) DMSO for 4 h at 65 °C. Lane M1, GeneRuler 1 kb DNA Ladder; lane M2, 100 bp Plus DNA Ladder; lane K, undigested DNA; lane 1, digestion in the presence of SAM; lane 2, digestion in the presence of DMSO; lane 3, digestion in the presence of both SAM and DMSO. b The same as ( a ), but SIN was used instead of SAM. c Distribution of insert lengths in 200 clones randomly selected from TthHB27I/DMSO/SAM-generated λ DNA library. d The same as ( c ), but for TthHB27I/DMSO/SIN-generated λ DNA library

    Journal: BMC Genomics

    Article Title: Randomized DNA libraries construction tool: a new 3-bp ‘frequent cutter’ TthHB27I/sinefungin endonuclease with chemically-induced specificity

    doi: 10.1186/s12864-018-4748-0

    Figure Lengend Snippet: Cleavage of λ DNA under TthHB27I specificity relaxation conditions. a Cleavage pattern of λ DNA digested with TthHB27I under various conditions. 0.5 μg of DNA substrate was digested with 2 U of TthHB27I in REase buffer supplemented with 100 μM SAM and/or 25% (v/v) DMSO for 4 h at 65 °C. Lane M1, GeneRuler 1 kb DNA Ladder; lane M2, 100 bp Plus DNA Ladder; lane K, undigested DNA; lane 1, digestion in the presence of SAM; lane 2, digestion in the presence of DMSO; lane 3, digestion in the presence of both SAM and DMSO. b The same as ( a ), but SIN was used instead of SAM. c Distribution of insert lengths in 200 clones randomly selected from TthHB27I/DMSO/SAM-generated λ DNA library. d The same as ( c ), but for TthHB27I/DMSO/SIN-generated λ DNA library

    Article Snippet: DNA isolation kit (GeneJet Plasmid Miniprep Kit), DNA markers (GeneRuler 1 kb DNA Ladder, 100 bp Plus DNA Ladder), SmaI REase, FastAP Thermosensitive Alkaline Phosphatase, T4 DNA Polymerase and T4 DNA Ligase were from Thermo Fisher Scientific/Fermentas (Vilnius, USA/Lithuania).

    Techniques: Clone Assay, Generated

    TthHB27I digestion patterns comparison in the presence of DMSO and cofactor SAM or its analogues. 0.5 μg of 1789 bp PCR DNA substrate (see Additional file 1 ) was incubated with 2 U of TthHB27I for 1 h at 65 °C in REase buffer with increasing amount of DMSO. Reaction mixtures were precipitated and electrophoresed in 1.3% agarose/TBE gels. The pictures on the right side of the figure show the theoretical arrangement of the DNA bands in an agarose gel after digesting the substrate DNA with TthHB27I under standard conditions. a Digestions performed only with the addition of DMSO, without any cofactor. Lane M1, GeneRuler 1 kb DNA Ladder; lane M2, 100 bp Plus DNA Ladder; lane K, undigested PCR fragment; lane 1, 0% ( v /v) DMSO present in the reaction mixture; lane 2, with 5% (v/v) DMSO; lane 3, with 10% (v/v) DMSO; lane 4, with 15% (v/v) DMSO; lane 5, with 20% (v/v) DMSO; lane 6, with 25% (v/v) DMSO; lane 7, with 30% (v/v) DMSO. As in ( a ), but in the presence of 100 μM SAM ( b ); 100 μM SIN ( c ); 100 μM SAC ( d ); 100 μM SAH ( e ); 100 μM ATP ( f )

    Journal: BMC Genomics

    Article Title: Randomized DNA libraries construction tool: a new 3-bp ‘frequent cutter’ TthHB27I/sinefungin endonuclease with chemically-induced specificity

    doi: 10.1186/s12864-018-4748-0

    Figure Lengend Snippet: TthHB27I digestion patterns comparison in the presence of DMSO and cofactor SAM or its analogues. 0.5 μg of 1789 bp PCR DNA substrate (see Additional file 1 ) was incubated with 2 U of TthHB27I for 1 h at 65 °C in REase buffer with increasing amount of DMSO. Reaction mixtures were precipitated and electrophoresed in 1.3% agarose/TBE gels. The pictures on the right side of the figure show the theoretical arrangement of the DNA bands in an agarose gel after digesting the substrate DNA with TthHB27I under standard conditions. a Digestions performed only with the addition of DMSO, without any cofactor. Lane M1, GeneRuler 1 kb DNA Ladder; lane M2, 100 bp Plus DNA Ladder; lane K, undigested PCR fragment; lane 1, 0% ( v /v) DMSO present in the reaction mixture; lane 2, with 5% (v/v) DMSO; lane 3, with 10% (v/v) DMSO; lane 4, with 15% (v/v) DMSO; lane 5, with 20% (v/v) DMSO; lane 6, with 25% (v/v) DMSO; lane 7, with 30% (v/v) DMSO. As in ( a ), but in the presence of 100 μM SAM ( b ); 100 μM SIN ( c ); 100 μM SAC ( d ); 100 μM SAH ( e ); 100 μM ATP ( f )

    Article Snippet: DNA isolation kit (GeneJet Plasmid Miniprep Kit), DNA markers (GeneRuler 1 kb DNA Ladder, 100 bp Plus DNA Ladder), SmaI REase, FastAP Thermosensitive Alkaline Phosphatase, T4 DNA Polymerase and T4 DNA Ligase were from Thermo Fisher Scientific/Fermentas (Vilnius, USA/Lithuania).

    Techniques: Polymerase Chain Reaction, Incubation, Agarose Gel Electrophoresis

    Controlling of partial cleavage of genomic DNAs by TthHB27I under cofactor/analogue-induced ‘star’ activity conditions. E. coli and λ genomic DNAs were digested with TthHB27I under cofactor/analogue-induced ‘star’ conditions, while varying: temperature, incubation time and TthHB27I amount. 0.5 μg of DNAs were digested with TthHB27I in REase buffer supplemented with 100 μM SIN and 25% (v/v) DMSO. a Cleavage control by reaction temperature. Reactions conducted with E. coli DNA digested with 2 U of TthHB27I for 1 h at temperatures from 30 °C to 80 °C. Lane M1, GeneRuler 1 kb DNA Ladder; lane M2, 100 bp Plus DNA Ladder; lane K, undigested DNA; lane 1, reaction at 30 °C; lane 2, 35 °C; lane 3, 40 °C; lane 4, 45 °C; lane 5, 50 °C; lane 6, 55 °C; lane 7, 60 °C; lane 8, 65 °C; lane 9, 70 °C; lane 10, 75 °C; lane 11, 80 °C. b The same as ( a ), except that λ DNA was used. c Cleavage control by reaction time. E. coli DNA was digested at 65 °C at times ranging from 7.5 min to 16 h. Lane M1, GeneRuler 1 kb DNA Ladder; lane M2, 100 bp Plus DNA Ladder; lane K, undigested DNA; lane 1, reaction conducted for 7.5 min; lane 2, 15 min; lane 3, 30 min; lane 4, 1 h; lane 5, 2 h; lane 6, 4 h; lane 7, 8 h; lane 8, 16 h. d The same as ( c ), except that λ DNA was used. e Cleavage control by the enzyme amount. E. coli DNA was digested at 65 °C with TthHB27I. Lane M1, GeneRuler 1 kb DNA Ladder; lane M2, 100 bp Plus DNA Ladder; lane K, undigested DNA; lane 1, DNA digested with 8 U TthHB27I; lane 2, 4 U; lane 3, 2 U; lane 4, 1 U; lane 5, 0.5 U; lane 6, 0.25 U; lane 7, 0.12 U; lane 8, 0.06 U. f The same as ( e ), except that λ DNA was used

    Journal: BMC Genomics

    Article Title: Randomized DNA libraries construction tool: a new 3-bp ‘frequent cutter’ TthHB27I/sinefungin endonuclease with chemically-induced specificity

    doi: 10.1186/s12864-018-4748-0

    Figure Lengend Snippet: Controlling of partial cleavage of genomic DNAs by TthHB27I under cofactor/analogue-induced ‘star’ activity conditions. E. coli and λ genomic DNAs were digested with TthHB27I under cofactor/analogue-induced ‘star’ conditions, while varying: temperature, incubation time and TthHB27I amount. 0.5 μg of DNAs were digested with TthHB27I in REase buffer supplemented with 100 μM SIN and 25% (v/v) DMSO. a Cleavage control by reaction temperature. Reactions conducted with E. coli DNA digested with 2 U of TthHB27I for 1 h at temperatures from 30 °C to 80 °C. Lane M1, GeneRuler 1 kb DNA Ladder; lane M2, 100 bp Plus DNA Ladder; lane K, undigested DNA; lane 1, reaction at 30 °C; lane 2, 35 °C; lane 3, 40 °C; lane 4, 45 °C; lane 5, 50 °C; lane 6, 55 °C; lane 7, 60 °C; lane 8, 65 °C; lane 9, 70 °C; lane 10, 75 °C; lane 11, 80 °C. b The same as ( a ), except that λ DNA was used. c Cleavage control by reaction time. E. coli DNA was digested at 65 °C at times ranging from 7.5 min to 16 h. Lane M1, GeneRuler 1 kb DNA Ladder; lane M2, 100 bp Plus DNA Ladder; lane K, undigested DNA; lane 1, reaction conducted for 7.5 min; lane 2, 15 min; lane 3, 30 min; lane 4, 1 h; lane 5, 2 h; lane 6, 4 h; lane 7, 8 h; lane 8, 16 h. d The same as ( c ), except that λ DNA was used. e Cleavage control by the enzyme amount. E. coli DNA was digested at 65 °C with TthHB27I. Lane M1, GeneRuler 1 kb DNA Ladder; lane M2, 100 bp Plus DNA Ladder; lane K, undigested DNA; lane 1, DNA digested with 8 U TthHB27I; lane 2, 4 U; lane 3, 2 U; lane 4, 1 U; lane 5, 0.5 U; lane 6, 0.25 U; lane 7, 0.12 U; lane 8, 0.06 U. f The same as ( e ), except that λ DNA was used

    Article Snippet: DNA isolation kit (GeneJet Plasmid Miniprep Kit), DNA markers (GeneRuler 1 kb DNA Ladder, 100 bp Plus DNA Ladder), SmaI REase, FastAP Thermosensitive Alkaline Phosphatase, T4 DNA Polymerase and T4 DNA Ligase were from Thermo Fisher Scientific/Fermentas (Vilnius, USA/Lithuania).

    Techniques: Activity Assay, Incubation