dna ladder  (Solis BioDyne)

Journal: Iranian Journal of Parasitology

Article Title: Frequency of Toxoplasma gondii in HIV Positive Patients from West of Iran by ELISA and PCR

doi:

Figure Lengend Snippet: Result of PCR from HIV/AIDS positive sera with T. gondii antigenemia from Sanandaj, Kordistan, west of Iran in 2011 Lane M: marker (Solis – Biodyne)/ Lanes 1 and 2: HIV- positive serum with T. gondii antigenemia, stained with etidium bromid and DNA safe stain, respectively./ Lane 3: Positive control (DNA of tachyzoites of T. gondii )/ Lanes 4 and 5: negative control serum.

Article Snippet: After an initial denaturation at 95°C for 10 min, 40 cycles were run, including denaturation (92°C for 30 sec), annealing (55°C for 50 sec), and extension (72°C for 30 sec) and final extension at 72°C for 7 min. PCR products and DNA ladder (Solis_Biodyne, Estonia) were electrophoresed in 1.5% agarose gel (Merck, Germany) and stained with ethidium bromide and DNA safestain.

Techniques: Polymerase Chain Reaction, Marker, Staining, Positive Control, Negative Control

Journal: Antioxidants

Article Title: Raspberry Ketones Attenuate Cyclophosphamide-Induced Pulmonary Toxicity in Mice through Inhibition of Oxidative Stress and NF-ΚB Pathway

doi: 10.3390/antiox9111168

Figure Lengend Snippet: Effect of CP (150 mg/kg/i.p) and different doses of RKs (25, 50, 100, 200 mg/kg/p.o )on the integrity of nuclear DNA (nDNA) extracted from lung tissues of male mice ( n = 3). Lane M, 100 kb DNA ladder; intact nDNA of Control (lane 1) and (lane 2) CP-treated mice, nDNA samples of RKs with different treated doses such as: RKs (25 mg/kg/p.o) of (lane 3), RKs (50 mg/kg/p.o) of (lane 4), RKs (100 mg/kg/p.o) of (lane 5), and finally RKs (200 mg/kg/p.o) of (lane 6) for 2 weeks before acute exposure to CP (150 mg/kg/p.o).

Article Snippet: The 100 bp DNA ladder (Solis Biodyn, Tartu, Estonia) was a ready-to-use molecular weight marker.

Techniques: Mouse Assay

Journal: PLoS ONE

Article Title: Design of a new multiplex PCR assay for rice pathogenic bacteria detection and its application to infer disease incidence and detect co-infection in rice fields in Burkina Faso

doi: 10.1371/journal.pone.0232115

Figure Lengend Snippet: Detection of the five bacterial taxa using the newly described multiplex PCR protocol. L: molecular size marker, 100 bp DNA ladder ready to load, Solis Biodyne, Pfs : P . fuscovaginae strain UBP735, Bg : B . glumae strain NCPPB 3923, Sph : Sphingomonas strain V1-2, Xoc : X . oryzae pv. oryzicola strain BAI10, Pan : Pantoea strain ARC10, Mix: Equal amounts of all five DNA samples.

Article Snippet: L: molecular size marker, 100pb DNA ladder ready to load, Solis Biodyne.

Techniques: Multiplex Assay, Polymerase Chain Reaction, Marker

Journal: Frontiers in Physiology

Article Title: Supplementing Blood Diet With Plant Nectar Enhances Egg Fertility in Stomoxys calcitrans

doi: 10.3389/fphys.2021.646367

Figure Lengend Snippet: Amplification of target chloroplast marker for identification of plant sources of nectar. (A) Electrophoresis of PCR amplicons resolved through 1% agarose gel stained with ethidium bromide for visualization of DNA bands. Positive (+ve) control was fall army warm larvae fed on maize; −ve control was PCR Mastermix with water used as template. Lanes Sc1–Sc8 represent Stomoxys calcitrans DNA samples amplified for chloroplast DNA. (B) Graph showing the percent plant-fed stable flies collected from three different sampling sites.

Article Snippet: Then, a 100-bp DNA ladder (Solis BioDyne, Estonia) was used to estimate the amplicon sizes.

Techniques: Amplification, Marker, Electrophoresis, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Staining, Sampling