dna ladder 100 bp  (New England Biolabs)


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    Name:
    100 bp DNA Ladder
    Description:
    100 bp DNA Ladder 500 gel lanes
    Catalog Number:
    n3231l
    Price:
    231
    Size:
    500 gel lanes
    Category:
    DNA Ladders
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    Structured Review

    New England Biolabs dna ladder 100 bp
    100 bp DNA Ladder
    100 bp DNA Ladder 500 gel lanes
    https://www.bioz.com/result/dna ladder 100 bp/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna ladder 100 bp - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: ADH1B, ALDH2, GSTM1 and GSTT1 Gene Polymorphic Frequencies among Alcoholics and Controls in the Arcadian Population of Central India
    Article Snippet: .. After PCR, amplicons size with the help of 100bp DNA ladder (NEB, US) were analyzed by gel electrophoresis using ethidium bromide (EtBr) stained (10mg/ml) 2% AG (Sigma). ..

    Agarose Gel Electrophoresis:

    Article Title: Identification of UDP Glycosyltransferase 3A1 as a UDPN-Acetylglucosaminyltransferase *
    Article Snippet: .. At the end of 40 cycles, the integrity of PCR products was assessed by electrophoresis on a 1.5% agarose gel with 100-bp DNA markers (New England Biolabs) as a reference to estimate molecular size. .. The DNA was visualized by staining with ethidium bromide.

    Electrophoretic Mobility Shift Assay:

    Article Title: Leishmania actin binds and nicks kDNA as well as inhibits decatenation activity of type II topoisomerase
    Article Snippet: .. For Electrophoretic mobility shift assay ( EMSA) on polyacrylamide gel, 100 ng of 30 bp DNA was 5′-end labeled with [γ-32 P] ATP using T4 polynucleotide kinase (NEB). .. Different concentrations of rLdACT were mixed with ∼5 pmol of 5′-end-labeled and purified DNA fragments in a total volume of 20 μl in 20 mM Tris–HCl, 0.1 mM EDTA, pH 7.5 and incubated for 30 min at 25°C.

    Electrophoresis:

    Article Title: Identification of UDP Glycosyltransferase 3A1 as a UDPN-Acetylglucosaminyltransferase *
    Article Snippet: .. At the end of 40 cycles, the integrity of PCR products was assessed by electrophoresis on a 1.5% agarose gel with 100-bp DNA markers (New England Biolabs) as a reference to estimate molecular size. .. The DNA was visualized by staining with ethidium bromide.

    Polymerase Chain Reaction:

    Article Title: ADH1B, ALDH2, GSTM1 and GSTT1 Gene Polymorphic Frequencies among Alcoholics and Controls in the Arcadian Population of Central India
    Article Snippet: .. After PCR, amplicons size with the help of 100bp DNA ladder (NEB, US) were analyzed by gel electrophoresis using ethidium bromide (EtBr) stained (10mg/ml) 2% AG (Sigma). ..

    Article Title: Nitrogen regulation of protein-protein interactions and transcript levels of GlnK PII regulator and AmtB ammonium transporter homologs in Archaea
    Article Snippet: .. PCR fragments were visualized in 1.8% (w/v) agarose gels stained with ethidium bromide and compared to the 100 bp DNA Ladder from New England Biolabs (Ipswich, MA). .. Cell fractionation Cells were harvested by centrifugation at 5500g for 15 min at 4°C (Avanti J-20 XP Centrifuge; Beckman Coulter, Brea, CA) and disrupted passing them twice at 2000 psi through a cooled French press (SLM AMINCO, Urbana, IL).

    Article Title: Identification of UDP Glycosyltransferase 3A1 as a UDPN-Acetylglucosaminyltransferase *
    Article Snippet: .. At the end of 40 cycles, the integrity of PCR products was assessed by electrophoresis on a 1.5% agarose gel with 100-bp DNA markers (New England Biolabs) as a reference to estimate molecular size. .. The DNA was visualized by staining with ethidium bromide.

    Labeling:

    Article Title: Leishmania actin binds and nicks kDNA as well as inhibits decatenation activity of type II topoisomerase
    Article Snippet: .. For Electrophoretic mobility shift assay ( EMSA) on polyacrylamide gel, 100 ng of 30 bp DNA was 5′-end labeled with [γ-32 P] ATP using T4 polynucleotide kinase (NEB). .. Different concentrations of rLdACT were mixed with ∼5 pmol of 5′-end-labeled and purified DNA fragments in a total volume of 20 μl in 20 mM Tris–HCl, 0.1 mM EDTA, pH 7.5 and incubated for 30 min at 25°C.

    Marker:

    Article Title: Identification of Coupling and Repulsion Phase DNA Marker Associated With an Allele of a Gene Conferring Host Plant Resistance to Pigeonpea sterility mosaic virus (PPSMV) in Pigeonpea (Cajanus cajan L. Millsp.)
    Article Snippet: .. The molecular weights of the short decamer random DNA marker products were estimated with a 100-bp DNA ladder (New England BioLabs, MA, USA). ..

    Article Title: Simple and Reliable Multiplex PCR Assay for Surveillance Isolates of Vancomycin-Resistant Enterococci
    Article Snippet: .. A 100-bp DNA ladder (New England Biolabs, Beverly, Mass.) was used as the molecular size marker. .. The gels were stained with ethidium bromide and photographed under UV light.

    Staining:

    Article Title: ADH1B, ALDH2, GSTM1 and GSTT1 Gene Polymorphic Frequencies among Alcoholics and Controls in the Arcadian Population of Central India
    Article Snippet: .. After PCR, amplicons size with the help of 100bp DNA ladder (NEB, US) were analyzed by gel electrophoresis using ethidium bromide (EtBr) stained (10mg/ml) 2% AG (Sigma). ..

    Article Title: Nitrogen regulation of protein-protein interactions and transcript levels of GlnK PII regulator and AmtB ammonium transporter homologs in Archaea
    Article Snippet: .. PCR fragments were visualized in 1.8% (w/v) agarose gels stained with ethidium bromide and compared to the 100 bp DNA Ladder from New England Biolabs (Ipswich, MA). .. Cell fractionation Cells were harvested by centrifugation at 5500g for 15 min at 4°C (Avanti J-20 XP Centrifuge; Beckman Coulter, Brea, CA) and disrupted passing them twice at 2000 psi through a cooled French press (SLM AMINCO, Urbana, IL).

    Molecular Weight:

    Article Title: RIPiT-Seq: A high-throughput approach for footprinting RNA:protein complexes
    Article Snippet: .. Oligos and ladders: Low molecular weight ssDNA ladder (USB, 76410), 100bp ladder (NEB, N3231S) Gel drying and imaging: 3M whatman filter paper, Saran wrap, Gel dryer, Phosphorimager screens, Typhoon scanner .. SYBR-Gold (Life technologies, S-11494) Blue-light LED transilluminator, ultrabright (New EnglandBio Group) RNA elution buffer (REB): 0.3M Sodium acetate pH 5.2, 0.1 mM EDTA Spin-X column (Corning, 8161)

    Imaging:

    Article Title: RIPiT-Seq: A high-throughput approach for footprinting RNA:protein complexes
    Article Snippet: .. Oligos and ladders: Low molecular weight ssDNA ladder (USB, 76410), 100bp ladder (NEB, N3231S) Gel drying and imaging: 3M whatman filter paper, Saran wrap, Gel dryer, Phosphorimager screens, Typhoon scanner .. SYBR-Gold (Life technologies, S-11494) Blue-light LED transilluminator, ultrabright (New EnglandBio Group) RNA elution buffer (REB): 0.3M Sodium acetate pH 5.2, 0.1 mM EDTA Spin-X column (Corning, 8161)

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  • 99
    New England Biolabs 100 bp dna ladder
    Segregation of coupling phase RAPD markers IABTPPN7 983 and repulsion phase RAPD marker IABTPPN7 414 across F 2 plant <t>DNA</t> of the cross Gullyal white × BSMR 736 linked to PSMD. P1=PSMD susceptible parent Gullyal white, P2=PSMD resistant parent BSMR 736, Lane <t>M=100</t> bp DNA ladder.
    100 Bp Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/100 bp dna ladder/product/New England Biolabs
    Average 99 stars, based on 43 article reviews
    Price from $9.99 to $1999.99
    100 bp dna ladder - by Bioz Stars, 2020-08
    99/100 stars
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    Segregation of coupling phase RAPD markers IABTPPN7 983 and repulsion phase RAPD marker IABTPPN7 414 across F 2 plant DNA of the cross Gullyal white × BSMR 736 linked to PSMD. P1=PSMD susceptible parent Gullyal white, P2=PSMD resistant parent BSMR 736, Lane M=100 bp DNA ladder.

    Journal: The Plant Pathology Journal

    Article Title: Identification of Coupling and Repulsion Phase DNA Marker Associated With an Allele of a Gene Conferring Host Plant Resistance to Pigeonpea sterility mosaic virus (PPSMV) in Pigeonpea (Cajanus cajan L. Millsp.)

    doi: 10.5423/PPJ.OA.07.2014.0064

    Figure Lengend Snippet: Segregation of coupling phase RAPD markers IABTPPN7 983 and repulsion phase RAPD marker IABTPPN7 414 across F 2 plant DNA of the cross Gullyal white × BSMR 736 linked to PSMD. P1=PSMD susceptible parent Gullyal white, P2=PSMD resistant parent BSMR 736, Lane M=100 bp DNA ladder.

    Article Snippet: The molecular weights of the short decamer random DNA marker products were estimated with a 100-bp DNA ladder (New England BioLabs, MA, USA).

    Techniques: Marker

    Screening of the seven resistant and seven susceptible F 2 plant DNA of the cross Gullyal white × BSMR 736 with a coupling phase RAPD markers IABTPPN7 983 and repulsion phase RAPD marker IABTPPN7 414 , linked to PSMD. SP=PSMD susceptible parent Gullyal white, RP=PSMD resistant parent BSMR 736, Lane M=100 bp DNA ladder.

    Journal: The Plant Pathology Journal

    Article Title: Identification of Coupling and Repulsion Phase DNA Marker Associated With an Allele of a Gene Conferring Host Plant Resistance to Pigeonpea sterility mosaic virus (PPSMV) in Pigeonpea (Cajanus cajan L. Millsp.)

    doi: 10.5423/PPJ.OA.07.2014.0064

    Figure Lengend Snippet: Screening of the seven resistant and seven susceptible F 2 plant DNA of the cross Gullyal white × BSMR 736 with a coupling phase RAPD markers IABTPPN7 983 and repulsion phase RAPD marker IABTPPN7 414 , linked to PSMD. SP=PSMD susceptible parent Gullyal white, RP=PSMD resistant parent BSMR 736, Lane M=100 bp DNA ladder.

    Article Snippet: The molecular weights of the short decamer random DNA marker products were estimated with a 100-bp DNA ladder (New England BioLabs, MA, USA).

    Techniques: Marker

    Amplification pattern of coupling phase RAPD markers IABTPPN7 983 and repulsion phase RAPD marker IABTPPN7 414 in parents and resistant and susceptible bulks. M, 100 bp ladder DNA; RP, Resistant parent - BSMR 736; RB, resistant bulk; SP, susceptible parent - Gullyal white; SB, susceptible bulk.

    Journal: The Plant Pathology Journal

    Article Title: Identification of Coupling and Repulsion Phase DNA Marker Associated With an Allele of a Gene Conferring Host Plant Resistance to Pigeonpea sterility mosaic virus (PPSMV) in Pigeonpea (Cajanus cajan L. Millsp.)

    doi: 10.5423/PPJ.OA.07.2014.0064

    Figure Lengend Snippet: Amplification pattern of coupling phase RAPD markers IABTPPN7 983 and repulsion phase RAPD marker IABTPPN7 414 in parents and resistant and susceptible bulks. M, 100 bp ladder DNA; RP, Resistant parent - BSMR 736; RB, resistant bulk; SP, susceptible parent - Gullyal white; SB, susceptible bulk.

    Article Snippet: The molecular weights of the short decamer random DNA marker products were estimated with a 100-bp DNA ladder (New England BioLabs, MA, USA).

    Techniques: Amplification, Marker

    Detection of UGT3A1 transcripts in a human tissue RNA panel. UGT3A1 RNA was amplified by reverse transcription-PCR over 40 cycles and detected by electrophoresis in a 1.5% agarose gel, stained with ethidium bromide. The 100-bp DNA markers are shown

    Journal: The Journal of Biological Chemistry

    Article Title: Identification of UDP Glycosyltransferase 3A1 as a UDPN-Acetylglucosaminyltransferase *

    doi: 10.1074/jbc.M807961200

    Figure Lengend Snippet: Detection of UGT3A1 transcripts in a human tissue RNA panel. UGT3A1 RNA was amplified by reverse transcription-PCR over 40 cycles and detected by electrophoresis in a 1.5% agarose gel, stained with ethidium bromide. The 100-bp DNA markers are shown

    Article Snippet: At the end of 40 cycles, the integrity of PCR products was assessed by electrophoresis on a 1.5% agarose gel with 100-bp DNA markers (New England Biolabs) as a reference to estimate molecular size.

    Techniques: Amplification, Polymerase Chain Reaction, Electrophoresis, Agarose Gel Electrophoresis, Staining

    For ADH1B Genotyping–Lane L, 100bp DNA Ladder; Lane 1–7, Undigested 155bp bands for ADH1B.

    Journal: Asian Pacific Journal of Cancer Prevention : APJCP

    Article Title: ADH1B, ALDH2, GSTM1 and GSTT1 Gene Polymorphic Frequencies among Alcoholics and Controls in the Arcadian Population of Central India

    doi: 10.22034/APJCP.2018.19.3.725

    Figure Lengend Snippet: For ADH1B Genotyping–Lane L, 100bp DNA Ladder; Lane 1–7, Undigested 155bp bands for ADH1B.

    Article Snippet: After PCR, amplicons size with the help of 100bp DNA ladder (NEB, US) were analyzed by gel electrophoresis using ethidium bromide (EtBr) stained (10mg/ml) 2% AG (Sigma).

    Techniques:

    For ALDH2 Genotyping–Lane L, 100bp DNA Ladder; Lane 1–2, Digested fragments of 90bp and 18bp bands; Lane 3–6, One uncut (119bp) with two digested fragments (90bp and 18bp); Lane 7, Undigested band (119bp).

    Journal: Asian Pacific Journal of Cancer Prevention : APJCP

    Article Title: ADH1B, ALDH2, GSTM1 and GSTT1 Gene Polymorphic Frequencies among Alcoholics and Controls in the Arcadian Population of Central India

    doi: 10.22034/APJCP.2018.19.3.725

    Figure Lengend Snippet: For ALDH2 Genotyping–Lane L, 100bp DNA Ladder; Lane 1–2, Digested fragments of 90bp and 18bp bands; Lane 3–6, One uncut (119bp) with two digested fragments (90bp and 18bp); Lane 7, Undigested band (119bp).

    Article Snippet: After PCR, amplicons size with the help of 100bp DNA ladder (NEB, US) were analyzed by gel electrophoresis using ethidium bromide (EtBr) stained (10mg/ml) 2% AG (Sigma).

    Techniques:

    GSTM1 Genotyping; Lane L, 100bp DNA Ladder; Lane 1, Null allele for GSTM1; Lane 2–6, Positive allele for GSTM1 (215bp).

    Journal: Asian Pacific Journal of Cancer Prevention : APJCP

    Article Title: ADH1B, ALDH2, GSTM1 and GSTT1 Gene Polymorphic Frequencies among Alcoholics and Controls in the Arcadian Population of Central India

    doi: 10.22034/APJCP.2018.19.3.725

    Figure Lengend Snippet: GSTM1 Genotyping; Lane L, 100bp DNA Ladder; Lane 1, Null allele for GSTM1; Lane 2–6, Positive allele for GSTM1 (215bp).

    Article Snippet: After PCR, amplicons size with the help of 100bp DNA ladder (NEB, US) were analyzed by gel electrophoresis using ethidium bromide (EtBr) stained (10mg/ml) 2% AG (Sigma).

    Techniques:

    ( A ) ChIP analysis using anti-rLdACT antibodies showed the in vivo association of LdACT with chromatin and kDNA network. ( a ) and ( b ) are the agarose gels of PCR products after ChIP assay, showing the association of LdACT with nuclear DNA and kDNA, respectively. Lanes are marked on the top with their respective antibodies used in the ChIP assay and arrows indicated the genes amplified after pull down. An irrelevant, non-DNA associating antibody, GRP78, was used as a negative control, whereas, antibodies against DNA polβ, and UMSBP (universal minicircle sequence-binding protein), were used as positive controls for nuclear DNA and kDNA respectively. LdPFN, Leishmania profilin; NM12/17, specific minicircle primers. ( B ) Agarose gel shift assay of supercoiled and linearized pBR322 (400 ng each) in the presence of rLdACT (0.5 2.0 μM), and β- and γ-actins (0.5 2.0 μM) as indicated on the top of the gels. Lane M, shows 1 kb DNA ladder; FI: supercoiled form, FII: relaxed form, FIII: linearized form of DNA. ( C ) Autoradiogram of EMSA on polyacrylamide gel of 32 P end-labelled 30 bp DNA probe in the presence of increasing concentration of rLdACT (0.1–0.6 μM).

    Journal: Nucleic Acids Research

    Article Title: Leishmania actin binds and nicks kDNA as well as inhibits decatenation activity of type II topoisomerase

    doi: 10.1093/nar/gkq051

    Figure Lengend Snippet: ( A ) ChIP analysis using anti-rLdACT antibodies showed the in vivo association of LdACT with chromatin and kDNA network. ( a ) and ( b ) are the agarose gels of PCR products after ChIP assay, showing the association of LdACT with nuclear DNA and kDNA, respectively. Lanes are marked on the top with their respective antibodies used in the ChIP assay and arrows indicated the genes amplified after pull down. An irrelevant, non-DNA associating antibody, GRP78, was used as a negative control, whereas, antibodies against DNA polβ, and UMSBP (universal minicircle sequence-binding protein), were used as positive controls for nuclear DNA and kDNA respectively. LdPFN, Leishmania profilin; NM12/17, specific minicircle primers. ( B ) Agarose gel shift assay of supercoiled and linearized pBR322 (400 ng each) in the presence of rLdACT (0.5 2.0 μM), and β- and γ-actins (0.5 2.0 μM) as indicated on the top of the gels. Lane M, shows 1 kb DNA ladder; FI: supercoiled form, FII: relaxed form, FIII: linearized form of DNA. ( C ) Autoradiogram of EMSA on polyacrylamide gel of 32 P end-labelled 30 bp DNA probe in the presence of increasing concentration of rLdACT (0.1–0.6 μM).

    Article Snippet: For Electrophoretic mobility shift assay ( EMSA) on polyacrylamide gel, 100 ng of 30 bp DNA was 5′-end labeled with [γ-32 P] ATP using T4 polynucleotide kinase (NEB).

    Techniques: Chromatin Immunoprecipitation, In Vivo, Polymerase Chain Reaction, Amplification, Negative Control, Sequencing, Binding Assay, Agarose Gel Electrophoresis, Shift Assay, Concentration Assay

    ( A ) Agarose gel (0.5%), showing the time dependent nicking of kDNA by rLdACT (4.0 μM) which revealed the existence of major nicked DNA and minor concatenated minicircle species. ( B ) Agarose gel, showing rLdACT mediated decatenation of the kDNA network in the presence or absence of anti-rLdACT antibodies. DM, decatenated kDNA marker (Topogen). ( C ) Agarose gel (1.0%), showing rLdACT mediated decatenation of kDNA network with rLdACT in the presence or absence of DNase-1 and its inhibitor EDTA, which completely rules out the possibility of DNA nicking by some contaminating nuclease. ( D ) Agarose gel (1.0%), showing requirement of rLdACT in its polymeric state for its kDNA decatenation activity. ( E ): ( a ), Agarose gel (1.0%), showing requirement of ATP in the rLdACT mediated kDNA decatenation process. ( b ), Graph, showing ATP dependence of rLdACT-mediated kDNA decatenation. ( F ) ( a ), Agarose gel (1.0%), showing rLdACT-mediated decatenation of kDNA in the presence of non-hydrolysable analogs of ATP. ( b ) Graph, showing relative inhibition of rLdACT mediated decatenation of kDNA network in the presence of non-hydrolysable ATP analogs when plotted with the increasing concentration of rLdACT.

    Journal: Nucleic Acids Research

    Article Title: Leishmania actin binds and nicks kDNA as well as inhibits decatenation activity of type II topoisomerase

    doi: 10.1093/nar/gkq051

    Figure Lengend Snippet: ( A ) Agarose gel (0.5%), showing the time dependent nicking of kDNA by rLdACT (4.0 μM) which revealed the existence of major nicked DNA and minor concatenated minicircle species. ( B ) Agarose gel, showing rLdACT mediated decatenation of the kDNA network in the presence or absence of anti-rLdACT antibodies. DM, decatenated kDNA marker (Topogen). ( C ) Agarose gel (1.0%), showing rLdACT mediated decatenation of kDNA network with rLdACT in the presence or absence of DNase-1 and its inhibitor EDTA, which completely rules out the possibility of DNA nicking by some contaminating nuclease. ( D ) Agarose gel (1.0%), showing requirement of rLdACT in its polymeric state for its kDNA decatenation activity. ( E ): ( a ), Agarose gel (1.0%), showing requirement of ATP in the rLdACT mediated kDNA decatenation process. ( b ), Graph, showing ATP dependence of rLdACT-mediated kDNA decatenation. ( F ) ( a ), Agarose gel (1.0%), showing rLdACT-mediated decatenation of kDNA in the presence of non-hydrolysable analogs of ATP. ( b ) Graph, showing relative inhibition of rLdACT mediated decatenation of kDNA network in the presence of non-hydrolysable ATP analogs when plotted with the increasing concentration of rLdACT.

    Article Snippet: For Electrophoretic mobility shift assay ( EMSA) on polyacrylamide gel, 100 ng of 30 bp DNA was 5′-end labeled with [γ-32 P] ATP using T4 polynucleotide kinase (NEB).

    Techniques: Agarose Gel Electrophoresis, Marker, Activity Assay, Inhibition, Concentration Assay

    ( A ) Agarose gel, showing supercoiled pBR322 DNA (400 ng) relaxation separately with rLdACT (0.5 1.0 μM) and E. coli Topo I. Image presented is the negative of original gel image. ( B ) Agarose gel electrophoresis of supercoiled pBR322 DNA (400 ng) with rLdACT in the presence or absence of anti-rLdACT antibodies, showing specificity of nicking activity associated with rLdACT. ( C ) Agarose gel electrophoresis of supercoiled pBR322 DNA (400 ng) with rLdACT (0.5 1.0 μM) in the presence or absence of DNase-1 and its inhibitor EDTA which further eliminates the possibility of DNA nicking by contaminating nuclease. ( D ): a and b, Agarose gel electrophoresis of supercoiled pBR322 DNA (400 ng) with rLdACT in the presence of ATP and its non-hydrolysable ATP analogs as indicated on the top of the gel. ( E ) Graph showing the requirement of ATP in its hydrolysable form during rLdACT mediated relaxation of supercoiled pBR322 DNA. ( F ) Agarose gel, showing requirement of rLdACT (0.5 2.0 μM) in its polymeric state for its scDNA-relaxation activity. ( G ) Graph, representing rLdACT (1.0 μM) mediated relaxation of supercoiled pBR322 DNA in the presence of increasing concentration of NaCl, inset shows the relative % inhibition of rLdACT mediated relaxation of supercoiled pBR322 DNA in presence of 50 mM salts having different ionization constant (Ksp) as indicated on the top of the bars. ( H ) Dynamic light scattering measurements of rLdACT (1.0 μM) showing insignificant effect of 0.2 M NaCl on the polymerized state of rLdACT after complete polymerization.

    Journal: Nucleic Acids Research

    Article Title: Leishmania actin binds and nicks kDNA as well as inhibits decatenation activity of type II topoisomerase

    doi: 10.1093/nar/gkq051

    Figure Lengend Snippet: ( A ) Agarose gel, showing supercoiled pBR322 DNA (400 ng) relaxation separately with rLdACT (0.5 1.0 μM) and E. coli Topo I. Image presented is the negative of original gel image. ( B ) Agarose gel electrophoresis of supercoiled pBR322 DNA (400 ng) with rLdACT in the presence or absence of anti-rLdACT antibodies, showing specificity of nicking activity associated with rLdACT. ( C ) Agarose gel electrophoresis of supercoiled pBR322 DNA (400 ng) with rLdACT (0.5 1.0 μM) in the presence or absence of DNase-1 and its inhibitor EDTA which further eliminates the possibility of DNA nicking by contaminating nuclease. ( D ): a and b, Agarose gel electrophoresis of supercoiled pBR322 DNA (400 ng) with rLdACT in the presence of ATP and its non-hydrolysable ATP analogs as indicated on the top of the gel. ( E ) Graph showing the requirement of ATP in its hydrolysable form during rLdACT mediated relaxation of supercoiled pBR322 DNA. ( F ) Agarose gel, showing requirement of rLdACT (0.5 2.0 μM) in its polymeric state for its scDNA-relaxation activity. ( G ) Graph, representing rLdACT (1.0 μM) mediated relaxation of supercoiled pBR322 DNA in the presence of increasing concentration of NaCl, inset shows the relative % inhibition of rLdACT mediated relaxation of supercoiled pBR322 DNA in presence of 50 mM salts having different ionization constant (Ksp) as indicated on the top of the bars. ( H ) Dynamic light scattering measurements of rLdACT (1.0 μM) showing insignificant effect of 0.2 M NaCl on the polymerized state of rLdACT after complete polymerization.

    Article Snippet: For Electrophoretic mobility shift assay ( EMSA) on polyacrylamide gel, 100 ng of 30 bp DNA was 5′-end labeled with [γ-32 P] ATP using T4 polynucleotide kinase (NEB).

    Techniques: Agarose Gel Electrophoresis, Activity Assay, Concentration Assay, Inhibition