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    Name:
    Illumina Tagment DNA Enzyme and Buffer Small Kit
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    Illumina Inc dna input
    Illumina Tagment DNA Enzyme and Buffer Small Kit

    https://www.bioz.com/result/dna input/product/Illumina Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna input - by Bioz Stars, 2021-07
    99/100 stars

    Images

    1) Product Images from "Advanced Whole Genome Sequencing Using a Complete PCR-free Massively Parallel Sequencing (MPS) Workflow"

    Article Title: Advanced Whole Genome Sequencing Using a Complete PCR-free Massively Parallel Sequencing (MPS) Workflow

    Journal: bioRxiv

    doi: 10.1101/2019.12.20.885517

    Coverage of the microbial genomes, Olsenella profusa (62% GC) (left) and Bacillus megaterium (38% GC) (right) with (A) MGIEasy PCR-Free DNA Library Pre Set and (B) MGIEasy FS PCR-Free DNA Library Pre Set. Read coverage across the range of the GC content, calculated in 100 bp windows (pink bars) and normalized coverage (colored dots). 3 replicates (red, blue and green dots) were included in the normalized coverage VS GC content analysis.
    Figure Legend Snippet: Coverage of the microbial genomes, Olsenella profusa (62% GC) (left) and Bacillus megaterium (38% GC) (right) with (A) MGIEasy PCR-Free DNA Library Pre Set and (B) MGIEasy FS PCR-Free DNA Library Pre Set. Read coverage across the range of the GC content, calculated in 100 bp windows (pink bars) and normalized coverage (colored dots). 3 replicates (red, blue and green dots) were included in the normalized coverage VS GC content analysis.

    Techniques Used: Polymerase Chain Reaction

    DNBSEQTM WGS PCR vs PCR-free workflows and general performance of PCR-free libraries. (a) MPS library construction workflows of WGS PCR and PCR-free libraries. RCA ( r olling c ircle a mplification) is used to increase signal intensity during array formation, which is followed by sequencing DNBs with DNBSEQ TM technology. Individual copies from the same DNB are replicated independently using the same ssCir template. Therefore, amplification errors cannot accumulate. Black, genomic DNA; Gray rectangle, adapter; green, barcode; red, amplification errors. (b) Two sets of 9 replicates from 1 μg NA12878 DNA were processed with MGIEasy PCR-Free DNA Library Prep Set (blue) or MGIEasy FS PCR-Free DNA Library Prep Set (orange). The GC content, Duplication rate, Median Insert size, and regions with > 10x Coverage were calculated and plotted. The error bars represent the standard deviations across the replicates.
    Figure Legend Snippet: DNBSEQTM WGS PCR vs PCR-free workflows and general performance of PCR-free libraries. (a) MPS library construction workflows of WGS PCR and PCR-free libraries. RCA ( r olling c ircle a mplification) is used to increase signal intensity during array formation, which is followed by sequencing DNBs with DNBSEQ TM technology. Individual copies from the same DNB are replicated independently using the same ssCir template. Therefore, amplification errors cannot accumulate. Black, genomic DNA; Gray rectangle, adapter; green, barcode; red, amplification errors. (b) Two sets of 9 replicates from 1 μg NA12878 DNA were processed with MGIEasy PCR-Free DNA Library Prep Set (blue) or MGIEasy FS PCR-Free DNA Library Prep Set (orange). The GC content, Duplication rate, Median Insert size, and regions with > 10x Coverage were calculated and plotted. The error bars represent the standard deviations across the replicates.

    Techniques Used: Polymerase Chain Reaction, Sequencing, Amplification

    2) Product Images from "Unstable Mechanisms of Resistance to Inhibitors of Escherichia coli Lipoprotein Signal Peptidase"

    Article Title: Unstable Mechanisms of Resistance to Inhibitors of Escherichia coli Lipoprotein Signal Peptidase

    Journal: mBio

    doi: 10.1128/mBio.02018-20

    Passaging of G0790-resistant strains containing GA in the absence of G0790 leads to loss of G0790 resistance. (A) Illumina coverage of lspA and surrounding genomic region in 4×-R3, 4×-R10, 8×-R5, Δ lpp 4×-R1, and Δ lpp 4×-R2. Regions with higher relative coverage than the surrounding region correspond to amplified DNA. The lspA gene is shown in green, and repeat sequences flanking the amplified region are indicated in red. (B) Schematic describing the passaging of G0790-resistant strains. G0790-resistant strains from either 4× or 8× MIC MHII agarose plates were passaged over 3 days in the presence (blue) or absence (pink) of G0790, details for which are included in Materials and Methods. (C) G0790 resistance in strains containing lspA _GA is lost after passaging for 3 days in the absence of G0790. Shown here are graphed MIC values (in mg/liter) after 3 days of passaging in the presence and absence of G0790 of WT CFT073 (■) strains containing lspA _GA (○; 4×-R3, 4×-R10, 8×-R1, and 8×-R5), lpp insertions (◊; 4×-R1, 4×-R6 and 4×-R7), or lpp deletions (△; 4×-R2, 4×-R4, 4×-R9, 4×-R1, 8×-R3, 8×-R4, and 8×-R6). Each symbol corresponds to an individual G0790-resistant strain, and the dotted lines correspond to the MIC of WT CFT073 (2.44 mg/liter). MIC data are averaged from duplicate wells and taken from two independent experiments.
    Figure Legend Snippet: Passaging of G0790-resistant strains containing GA in the absence of G0790 leads to loss of G0790 resistance. (A) Illumina coverage of lspA and surrounding genomic region in 4×-R3, 4×-R10, 8×-R5, Δ lpp 4×-R1, and Δ lpp 4×-R2. Regions with higher relative coverage than the surrounding region correspond to amplified DNA. The lspA gene is shown in green, and repeat sequences flanking the amplified region are indicated in red. (B) Schematic describing the passaging of G0790-resistant strains. G0790-resistant strains from either 4× or 8× MIC MHII agarose plates were passaged over 3 days in the presence (blue) or absence (pink) of G0790, details for which are included in Materials and Methods. (C) G0790 resistance in strains containing lspA _GA is lost after passaging for 3 days in the absence of G0790. Shown here are graphed MIC values (in mg/liter) after 3 days of passaging in the presence and absence of G0790 of WT CFT073 (■) strains containing lspA _GA (○; 4×-R3, 4×-R10, 8×-R1, and 8×-R5), lpp insertions (◊; 4×-R1, 4×-R6 and 4×-R7), or lpp deletions (△; 4×-R2, 4×-R4, 4×-R9, 4×-R1, 8×-R3, 8×-R4, and 8×-R6). Each symbol corresponds to an individual G0790-resistant strain, and the dotted lines correspond to the MIC of WT CFT073 (2.44 mg/liter). MIC data are averaged from duplicate wells and taken from two independent experiments.

    Techniques Used: Passaging, Amplification

    Related Articles

    Incubation:

    Article Title: Chemical genomics reveals histone deacetylases are required for core regulatory transcription
    Article Snippet: Briefly, 50,000 cells were isolated, and nuclei were generated by incubating on ice with 500 μL lysis buffer (RSB with 0.1% Tween-20) for 10 min. .. The resulting nuclei were centrifuged at 500 × g for 10 min, and resuspended in 1X Tagment DNA buffer (Illumina) with 2.5 μL Tagment DNA Enzyme (Illumina) and incubated at 37 °C for 30 min. For each transposition reaction, the volume was 50 μL. .. The transposition mixtures were quenched with 500 μL PB buffer (Qiagen) and purified by standard protocol with MinElute PCR purification kit.

    Polymerase Chain Reaction:

    Article Title: Exposure of Salmonella biofilms to antibiotic concentrations rapidly selects resistance with collateral tradeoffs
    Article Snippet: .. A measure of 0.9 µL of TD Tagment DNA Buffer (Illumina, Catalogue No. 15027866) was mixed with 0.09 µL TDE1, Tagment DNA Enzyme (Illumina, Catalogue No. 15027865) and 2.01 µL PCR grade water in a master mix and 3 µl added to a chilled 96-well plate. .. Two microlitres of normalised DNA (1 ng total) was mixed with the 3 µL of the Tagmentation mix and heated to 55 °C for 10 min in a PCR block.

    Purification:

    Article Title: Structure and mutagenic analysis of the lipid II flippase MurJ from Escherichia coli
    Article Snippet: The 2-kb fragments released were gel purified (Zymogen), and the amount of DNA recovered was quantitated using Qubit reactions (Invitrogen) according to the manufacturer’s protocol. .. After diluting the purified DNA samples to 2 ng/µL, 1 µL of the diluted DNA was mixed with 1.25 µL of Tagment DNA Buffer (15027866; Illumina) and 0.25 µL of Tagment DNA Enzyme (15027865; Illumina). .. The mixture was incubated for 10 min at 55 °C in a thermocycler.

    Lysis:

    Article Title: Impact of sustained TGFβ receptor inhibition on chromatin accessibility and gene expression in cultured human endometrial MSC
    Article Snippet: Briefly, cells were washed with cold PBS, lysed using cold EZ lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2 and 0.1% IGEPAL CA-630, Sigma-Aldrich, UK), transferred to chilled nuclease-free tubes, vortexed, left on ice for 5 minutes, and then pelleted in a refrigerated centrifuge. .. The nuclear pellet was washed in EZ lysis buffer and re-suspended in the transposase reaction mix containing 25 μl Tagment DNA (TD) Buffer, 5 μl Tagment DNA Enzyme and 20 μl nuclease free water (Nextera DNA Sample Preparation Kit, Illumina, UK) for 45 min at 37°C. .. Samples were purified using a Zymo DNA Clean and Concentrator-5 Purification kit (Zymo Research, USA).

    Sample Prep:

    Article Title: Impact of sustained TGFβ receptor inhibition on chromatin accessibility and gene expression in cultured human endometrial MSC
    Article Snippet: Briefly, cells were washed with cold PBS, lysed using cold EZ lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2 and 0.1% IGEPAL CA-630, Sigma-Aldrich, UK), transferred to chilled nuclease-free tubes, vortexed, left on ice for 5 minutes, and then pelleted in a refrigerated centrifuge. .. The nuclear pellet was washed in EZ lysis buffer and re-suspended in the transposase reaction mix containing 25 μl Tagment DNA (TD) Buffer, 5 μl Tagment DNA Enzyme and 20 μl nuclease free water (Nextera DNA Sample Preparation Kit, Illumina, UK) for 45 min at 37°C. .. Samples were purified using a Zymo DNA Clean and Concentrator-5 Purification kit (Zymo Research, USA).

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    Illumina Inc input dna libraries
    Comparison of <t>DNA</t> methylation state in FGSCs and MGSCs. a A snapshot of the IGV depicting DNA methylation in FGSCs and MGSCs. b Comparison of methylation at promoter regions (−2 kb to 500 bp) between FGSCs and MGSCs. We divided UCSC transcript promoters into 500-bp windows and showed that the absolute methylation signals from mean whole-genome bisulfite sequencing (MGSCs) and the mean <t>MethylCap-seq</t> normalized relative methylation levels (FGSCs) have a correlation of 0.229. c The number of genes methylated at TSS regions (−2 kb to 500 bp) in FGSCs and MGSCs. d Functional annotation of genes with FGSC-specific ( left ) or MGSC-specific methylation ( right ) ( p
    Input Dna Libraries, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/input dna libraries/product/Illumina Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    input dna libraries - by Bioz Stars, 2021-07
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    99
    Illumina Inc dna input
    Coverage of the microbial genomes, Olsenella profusa (62% GC) (left) and Bacillus megaterium (38% GC) (right) with (A) <t>MGIEasy</t> PCR-Free <t>DNA</t> Library Pre Set and (B) MGIEasy FS PCR-Free DNA Library Pre Set. Read coverage across the range of the GC content, calculated in 100 bp windows (pink bars) and normalized coverage (colored dots). 3 replicates (red, blue and green dots) were included in the normalized coverage VS GC content analysis.
    Dna Input, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna input/product/Illumina Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna input - by Bioz Stars, 2021-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    Comparison of DNA methylation state in FGSCs and MGSCs. a A snapshot of the IGV depicting DNA methylation in FGSCs and MGSCs. b Comparison of methylation at promoter regions (−2 kb to 500 bp) between FGSCs and MGSCs. We divided UCSC transcript promoters into 500-bp windows and showed that the absolute methylation signals from mean whole-genome bisulfite sequencing (MGSCs) and the mean MethylCap-seq normalized relative methylation levels (FGSCs) have a correlation of 0.229. c The number of genes methylated at TSS regions (−2 kb to 500 bp) in FGSCs and MGSCs. d Functional annotation of genes with FGSC-specific ( left ) or MGSC-specific methylation ( right ) ( p

    Journal: Genome Biology

    Article Title: Integrative epigenomic analysis reveals unique epigenetic signatures involved in unipotency of mouse female germline stem cells

    doi: 10.1186/s13059-016-1023-z

    Figure Lengend Snippet: Comparison of DNA methylation state in FGSCs and MGSCs. a A snapshot of the IGV depicting DNA methylation in FGSCs and MGSCs. b Comparison of methylation at promoter regions (−2 kb to 500 bp) between FGSCs and MGSCs. We divided UCSC transcript promoters into 500-bp windows and showed that the absolute methylation signals from mean whole-genome bisulfite sequencing (MGSCs) and the mean MethylCap-seq normalized relative methylation levels (FGSCs) have a correlation of 0.229. c The number of genes methylated at TSS regions (−2 kb to 500 bp) in FGSCs and MGSCs. d Functional annotation of genes with FGSC-specific ( left ) or MGSC-specific methylation ( right ) ( p

    Article Snippet: MethylCap and input DNA libraries were sequenced with an Illumina HiSeq 2000.

    Techniques: DNA Methylation Assay, Methylation, Methylation Sequencing, Functional Assay

    Comparison of input DNA signal tracks among all four barcoded adapters relative to standard Illumina adapters . An input sample was split in five aliquots. Four were barcoded differentially (top four lanes) and one had non-barcoded, Illumina adapters (fifth lane, labeled 'None'). Barcoded inputs were scored against non-barcoded input. IGB signal tracks of yeast chromosome 16 are shown for each sample, with ORF locations on the x-axis. ORFs are depicted in purple. On the y-axis, a normalized scale represents the number of read counts at a particular location. Each scale is normalized according to the number of mapped reads (Table 10 ). A box in the left panel depicts the enlarged section shown in the right panel for positions between 828,000 and 833,000 to demonstrate the overlap among all signal tracks.

    Journal: BMC Genomics

    Article Title: Efficient yeast ChIP-Seq using multiplex short-read DNA sequencing

    doi: 10.1186/1471-2164-10-37

    Figure Lengend Snippet: Comparison of input DNA signal tracks among all four barcoded adapters relative to standard Illumina adapters . An input sample was split in five aliquots. Four were barcoded differentially (top four lanes) and one had non-barcoded, Illumina adapters (fifth lane, labeled 'None'). Barcoded inputs were scored against non-barcoded input. IGB signal tracks of yeast chromosome 16 are shown for each sample, with ORF locations on the x-axis. ORFs are depicted in purple. On the y-axis, a normalized scale represents the number of read counts at a particular location. Each scale is normalized according to the number of mapped reads (Table 10 ). A box in the left panel depicts the enlarged section shown in the right panel for positions between 828,000 and 833,000 to demonstrate the overlap among all signal tracks.

    Article Snippet: For input DNA libraries, the Illumina genomic DNA adapters were diluted 1:20 and all barcoded adapters were diluted 1:30.

    Techniques: Labeling

    Barcoded adapters perform similarly to standard Illumina adapters and do not crossover to other samples in the same lane . (a) RNA PolII binding profiles from different biological replicates with the same barcode (PolII_Rep1, dark blue; PolII_Rep3, red), with different barcodes (PolII_Rep2, orange) or without barcode (PolII_Rep4, green) strongly overlap. See also Table 3 . Input DNA serves as a reference (light blue). IGB signal tracks of chromosome 5 between 130,000 and 320,000 are shown for each library. A box in the left panel depicts the enlarged section shown in the right panel between positions 298,000 and 309,000 to illustrate the overlap among all PolII signal tracks. (b) Binding profiles from four different libraries pooled and sequenced in the same flowcell lane show very little resemblance. Shown here are the binding profiles for Cse4_Rep2 (dark blue), Ste12_Rep2 (red), PolII_Rep2 (green) and Input_ACGT (light blue). IGB signal tracks of chromosome 12 between 80,000 and 210,000 are shown for each sample. For (a) and (b), axis and scale normalizations are similar to Figure 2 . (c) Left: Rank-rank comparison of target lists between all pairwise barcoded replicates for Cse4, PolII and Ste12. The horizontal axis shows the fraction of the two lists being compared and the vertical axis shows the fraction of those targets that agree between a given pair of target lists. All comparisons show strong agreement, although the rank lists for Cse4 differ more than PolII or Ste12 for the second half of their length. Right: Rank-rank comparison between barcoded replicates from the same factors (averaged over all pairwise comparisons) compared to rank-rank comparisons for barcoded replicates between different factors: PolII_Rep1 (ACGT) vs. Ste12_Rep1 (TGCT) and Cse4_Rep2 (CATT) vs. Ste12_Rep2 (GTAT).

    Journal: BMC Genomics

    Article Title: Efficient yeast ChIP-Seq using multiplex short-read DNA sequencing

    doi: 10.1186/1471-2164-10-37

    Figure Lengend Snippet: Barcoded adapters perform similarly to standard Illumina adapters and do not crossover to other samples in the same lane . (a) RNA PolII binding profiles from different biological replicates with the same barcode (PolII_Rep1, dark blue; PolII_Rep3, red), with different barcodes (PolII_Rep2, orange) or without barcode (PolII_Rep4, green) strongly overlap. See also Table 3 . Input DNA serves as a reference (light blue). IGB signal tracks of chromosome 5 between 130,000 and 320,000 are shown for each library. A box in the left panel depicts the enlarged section shown in the right panel between positions 298,000 and 309,000 to illustrate the overlap among all PolII signal tracks. (b) Binding profiles from four different libraries pooled and sequenced in the same flowcell lane show very little resemblance. Shown here are the binding profiles for Cse4_Rep2 (dark blue), Ste12_Rep2 (red), PolII_Rep2 (green) and Input_ACGT (light blue). IGB signal tracks of chromosome 12 between 80,000 and 210,000 are shown for each sample. For (a) and (b), axis and scale normalizations are similar to Figure 2 . (c) Left: Rank-rank comparison of target lists between all pairwise barcoded replicates for Cse4, PolII and Ste12. The horizontal axis shows the fraction of the two lists being compared and the vertical axis shows the fraction of those targets that agree between a given pair of target lists. All comparisons show strong agreement, although the rank lists for Cse4 differ more than PolII or Ste12 for the second half of their length. Right: Rank-rank comparison between barcoded replicates from the same factors (averaged over all pairwise comparisons) compared to rank-rank comparisons for barcoded replicates between different factors: PolII_Rep1 (ACGT) vs. Ste12_Rep1 (TGCT) and Cse4_Rep2 (CATT) vs. Ste12_Rep2 (GTAT).

    Article Snippet: For input DNA libraries, the Illumina genomic DNA adapters were diluted 1:20 and all barcoded adapters were diluted 1:30.

    Techniques: Binding Assay

    Scheme for yeast barcoded ChIP-Seq . (a) Barcoded ChIP-Seq workflow. Ovals depict yeast cells and squares depict proteins. An aliquot of sheared cell lysate is not immunoprecipitated but is otherwise processed normally (green). This DNA, termed input DNA, is a reference sample for ChIP-Seq. Illumina DNA libraries are generated from both ChIP and input DNA samples. In multiplex ChIP-Seq, a barcoded adapter is ligated to an individual DNA sample. The barcode has 3 unique bases followed by a 'T' to anneal with the end-repaired DNA. Four libraries are then pooled together and applied to a single flowcell lane. After sequencing on the Genome Analyzer, reads are separated according to the first four bases and aligned to the yeast genome. Reads are stacked to generate a signal profile and scored against a pool of input DNA to determine significant transcription factor binding sites. (b) The barcode (orange) is located between Illumina adapter sequences (purple) and ChIP or input DNA inserts (black). The sequencing primer (pink) anneals to the adapter sequences and short reads start with the four bases of the barcode (orange) followed by DNA inserts (black). For the sequencing primer and Illumina adapter, oligonucleotide sequences were given by the manufacturer © 2006 Illumina, Inc. All rights reserved.

    Journal: BMC Genomics

    Article Title: Efficient yeast ChIP-Seq using multiplex short-read DNA sequencing

    doi: 10.1186/1471-2164-10-37

    Figure Lengend Snippet: Scheme for yeast barcoded ChIP-Seq . (a) Barcoded ChIP-Seq workflow. Ovals depict yeast cells and squares depict proteins. An aliquot of sheared cell lysate is not immunoprecipitated but is otherwise processed normally (green). This DNA, termed input DNA, is a reference sample for ChIP-Seq. Illumina DNA libraries are generated from both ChIP and input DNA samples. In multiplex ChIP-Seq, a barcoded adapter is ligated to an individual DNA sample. The barcode has 3 unique bases followed by a 'T' to anneal with the end-repaired DNA. Four libraries are then pooled together and applied to a single flowcell lane. After sequencing on the Genome Analyzer, reads are separated according to the first four bases and aligned to the yeast genome. Reads are stacked to generate a signal profile and scored against a pool of input DNA to determine significant transcription factor binding sites. (b) The barcode (orange) is located between Illumina adapter sequences (purple) and ChIP or input DNA inserts (black). The sequencing primer (pink) anneals to the adapter sequences and short reads start with the four bases of the barcode (orange) followed by DNA inserts (black). For the sequencing primer and Illumina adapter, oligonucleotide sequences were given by the manufacturer © 2006 Illumina, Inc. All rights reserved.

    Article Snippet: For input DNA libraries, the Illumina genomic DNA adapters were diluted 1:20 and all barcoded adapters were diluted 1:30.

    Techniques: Chromatin Immunoprecipitation, Immunoprecipitation, Generated, Multiplex Assay, Sequencing, Binding Assay

    Fitness profile of influenza A virus segment 2 at single-nucleotide resolution. (A) Schematic representation of the experimental flow of high-throughput fitness profiling. Random single nucleotide mutations were introduced into influenza A/WSN/33 virus segment 2. Mutant viral libraries were generated by cotransfecting a mutant DNA library with seven plasmids encoding the other wild-type viral fragments. Viral libraries were then passaged in A549 cells. High-throughput sequencing of the plasmid mutant libraries and posttransfection and postinfection viral libraries was performed. (B) Correlation of the relative frequency of each single-nucleotide mutation between biological duplicates. (C) RF scores of individual mutations of influenza A/WSN/33 virus segment 2 in log 10 . Two representative regions are zoomed in on to show the single nucleotide change.

    Journal: mBio

    Article Title: Annotating Protein Functional Residues by Coupling High-Throughput Fitness Profile and Homologous-Structure Analysis

    doi: 10.1128/mBio.01801-16

    Figure Lengend Snippet: Fitness profile of influenza A virus segment 2 at single-nucleotide resolution. (A) Schematic representation of the experimental flow of high-throughput fitness profiling. Random single nucleotide mutations were introduced into influenza A/WSN/33 virus segment 2. Mutant viral libraries were generated by cotransfecting a mutant DNA library with seven plasmids encoding the other wild-type viral fragments. Viral libraries were then passaged in A549 cells. High-throughput sequencing of the plasmid mutant libraries and posttransfection and postinfection viral libraries was performed. (B) Correlation of the relative frequency of each single-nucleotide mutation between biological duplicates. (C) RF scores of individual mutations of influenza A/WSN/33 virus segment 2 in log 10 . Two representative regions are zoomed in on to show the single nucleotide change.

    Article Snippet: The input DNA libraries, posttransfection libraries, and postinfection libraries were subjected to Illumina sequencing.

    Techniques: Flow Cytometry, High Throughput Screening Assay, Mutagenesis, Generated, Next-Generation Sequencing, Plasmid Preparation

    Coverage of the microbial genomes, Olsenella profusa (62% GC) (left) and Bacillus megaterium (38% GC) (right) with (A) MGIEasy PCR-Free DNA Library Pre Set and (B) MGIEasy FS PCR-Free DNA Library Pre Set. Read coverage across the range of the GC content, calculated in 100 bp windows (pink bars) and normalized coverage (colored dots). 3 replicates (red, blue and green dots) were included in the normalized coverage VS GC content analysis.

    Journal: bioRxiv

    Article Title: Advanced Whole Genome Sequencing Using a Complete PCR-free Massively Parallel Sequencing (MPS) Workflow

    doi: 10.1101/2019.12.20.885517

    Figure Lengend Snippet: Coverage of the microbial genomes, Olsenella profusa (62% GC) (left) and Bacillus megaterium (38% GC) (right) with (A) MGIEasy PCR-Free DNA Library Pre Set and (B) MGIEasy FS PCR-Free DNA Library Pre Set. Read coverage across the range of the GC content, calculated in 100 bp windows (pink bars) and normalized coverage (colored dots). 3 replicates (red, blue and green dots) were included in the normalized coverage VS GC content analysis.

    Article Snippet: It should be noted that MGIEasy kit prepared libraries used 1 μg or 250ng DNA input, far less than the input amount of datasets downloaded from Illumina Basespace website.

    Techniques: Polymerase Chain Reaction

    DNBSEQTM WGS PCR vs PCR-free workflows and general performance of PCR-free libraries. (a) MPS library construction workflows of WGS PCR and PCR-free libraries. RCA ( r olling c ircle a mplification) is used to increase signal intensity during array formation, which is followed by sequencing DNBs with DNBSEQ TM technology. Individual copies from the same DNB are replicated independently using the same ssCir template. Therefore, amplification errors cannot accumulate. Black, genomic DNA; Gray rectangle, adapter; green, barcode; red, amplification errors. (b) Two sets of 9 replicates from 1 μg NA12878 DNA were processed with MGIEasy PCR-Free DNA Library Prep Set (blue) or MGIEasy FS PCR-Free DNA Library Prep Set (orange). The GC content, Duplication rate, Median Insert size, and regions with > 10x Coverage were calculated and plotted. The error bars represent the standard deviations across the replicates.

    Journal: bioRxiv

    Article Title: Advanced Whole Genome Sequencing Using a Complete PCR-free Massively Parallel Sequencing (MPS) Workflow

    doi: 10.1101/2019.12.20.885517

    Figure Lengend Snippet: DNBSEQTM WGS PCR vs PCR-free workflows and general performance of PCR-free libraries. (a) MPS library construction workflows of WGS PCR and PCR-free libraries. RCA ( r olling c ircle a mplification) is used to increase signal intensity during array formation, which is followed by sequencing DNBs with DNBSEQ TM technology. Individual copies from the same DNB are replicated independently using the same ssCir template. Therefore, amplification errors cannot accumulate. Black, genomic DNA; Gray rectangle, adapter; green, barcode; red, amplification errors. (b) Two sets of 9 replicates from 1 μg NA12878 DNA were processed with MGIEasy PCR-Free DNA Library Prep Set (blue) or MGIEasy FS PCR-Free DNA Library Prep Set (orange). The GC content, Duplication rate, Median Insert size, and regions with > 10x Coverage were calculated and plotted. The error bars represent the standard deviations across the replicates.

    Article Snippet: It should be noted that MGIEasy kit prepared libraries used 1 μg or 250ng DNA input, far less than the input amount of datasets downloaded from Illumina Basespace website.

    Techniques: Polymerase Chain Reaction, Sequencing, Amplification