dna fragments  (Qiagen)


Bioz Verified Symbol Qiagen is a verified supplier
Bioz Manufacturer Symbol Qiagen manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Name:
    QIAquick Gel Extraction Kit
    Description:
    For gel extraction cleanup of up to 10 μg DNA 70 bp to 10 kb from enzymatic reactions Kit contents Qiagen QIAquick Gel Extraction Kit 50 rxns 30 to 50L Elution Volume 10g Binding Capacity DNA Sample Tube Format Silica Technology Manual Processing 70 bp to 10 kb Fragment Fast and Convenient Procedure For Gel Extraction Cleanup of up to 10μg DNA 70 bp to 10 kb from Enzymatic Reactions Includes 50 QIAquick Spin Columns Buffers Collection Tubes 2mL Benefits Up to 95 recovery of ready to use DNA Fast and convenient procedure Cleanup of DNA up to 10 kb in three easy steps Gel loading dye for convenient sample analysis
    Catalog Number:
    28704
    Price:
    118
    Category:
    QIAquick Gel Extraction Kit
    Buy from Supplier


    Structured Review

    Qiagen dna fragments
    QIAquick Gel Extraction Kit
    For gel extraction cleanup of up to 10 μg DNA 70 bp to 10 kb from enzymatic reactions Kit contents Qiagen QIAquick Gel Extraction Kit 50 rxns 30 to 50L Elution Volume 10g Binding Capacity DNA Sample Tube Format Silica Technology Manual Processing 70 bp to 10 kb Fragment Fast and Convenient Procedure For Gel Extraction Cleanup of up to 10μg DNA 70 bp to 10 kb from Enzymatic Reactions Includes 50 QIAquick Spin Columns Buffers Collection Tubes 2mL Benefits Up to 95 recovery of ready to use DNA Fast and convenient procedure Cleanup of DNA up to 10 kb in three easy steps Gel loading dye for convenient sample analysis
    https://www.bioz.com/result/dna fragments/product/Qiagen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna fragments - by Bioz Stars, 2021-04
    86/100 stars

    Images

    Related Articles

    DNA Extraction:

    Article Title: Development of an Optimized MALDI-TOF-MS Method for High-Throughput Identification of High-Molecular-Weight Glutenin Subunits in Wheat
    Article Snippet: Ten μL of each sample was injected and eluted at 0.8 mL/min using a linear gradient of 23–44% of solvent B for 70 min. .. Wheat DNA Extraction and PCR Analysis Genomic DNA was extracted from 50 mg of wheat flour for the standard cultivars using a DNeasy® Plant Mini Kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions. .. Wheat DNA Extraction and PCR Analysis Genomic DNA was extracted from 50 mg of wheat flour for the standard cultivars using a DNeasy® Plant Mini Kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions.

    Polymerase Chain Reaction:

    Article Title: Development of an Optimized MALDI-TOF-MS Method for High-Throughput Identification of High-Molecular-Weight Glutenin Subunits in Wheat
    Article Snippet: Ten μL of each sample was injected and eluted at 0.8 mL/min using a linear gradient of 23–44% of solvent B for 70 min. .. Wheat DNA Extraction and PCR Analysis Genomic DNA was extracted from 50 mg of wheat flour for the standard cultivars using a DNeasy® Plant Mini Kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions. .. Wheat DNA Extraction and PCR Analysis Genomic DNA was extracted from 50 mg of wheat flour for the standard cultivars using a DNeasy® Plant Mini Kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions.

    Article Title: A Novel Set of Cas9 Fusion Proteins to stimulate Homologous Recombination: Cas9-HRs
    Article Snippet: Purification protocols were based on a modified version of a previously published two-step Cas9 purification protocol . .. Cas9-HR and Cas9 in-vitro exonuclease assay DNA was isolated from wildtype K562 cells, and amplified using standard Taq DNA polymerase and HBB-out-F (5’-aacgatcctgagacttccaca-3’) and HBB-out-R (5’-tgcttaccaagctgtgattcc-3’), Tm=56 for 35 cycles, and purified using the Quiagen PCR cleanup kit. .. Cas9-HR 3,4,8 or Cas9 were combined with the amplified HBB fragment at a 10:1 molar ratio (30nM: 3nM) in 1X Cas9 reaction Buffer (50mM Tris, 100mM NaCl, 10mM MgCl2, 1mM DTT, pH7.9) and incubated for 1hr at 37°C, after which 1µL of Proteinase K (NEB) was added and the reaction was incubated for an additional 20 minutes at 65°C.

    Article Title: Development of Genomic Array Footprinting for Identification of Conditionally Essential Genes in Streptococcus pneumoniae ▿ ▿ †
    Article Snippet: Ligated DNA was used as a template in two consecutive PCRs with Taq DNA polymerase: the first reaction contained primers LinkAmp1 (Table ) and PBMrTn5 (Table ), and the second contained nested PCR primers LinkAmp2 (Table ) and PBMrTn3 (Table ). .. PCR cycling conditions for both reactions were as follows: 94°C for 4 min; 20 cycles of 94°C for 30 s, 63°C for 1 min, and 72°C for 1.5 min; and 72°C for 3 min. PCR products were loaded on a 2% agarose gel, and DNA fragments with the preferred size were excised and extracted with a Qiaquick gel purification kit (QIAGEN). .. In vitro transcription reaction and subsequent DNase I digestion were performed with a T7 MEGAscript kit (Ambion).

    Article Title: Biliary tract instillation of a SMAC mimetic induces TRAIL-dependent acute sclerosing cholangitis-like injury in mice
    Article Snippet: Serum analysis Serum alanine aminotransferase (ALT), alkaline phosphatase, total bile acids and total bilirubin were measured using a commercially available veterinary chemistry analyzer (VetScan 2, Abaxis, Union City, CA, USA). .. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) Total RNA was isolated from cell cultures and frozen liver tissue using RNeasy Plus mini kit (Qiagen, Hilden, Germany). .. Reverse transcription was performed using Moloney murine leukemia virus reverse transcriptase and random primers (both Invitrogen, Carlsbad, CA, USA).

    Article Title: MicroRNA-126 regulates the phosphatidylinositol-3 kinase (PI3K)/protein kinase B (AKT) pathway in SLK cells in vitro and the expression of its pathway members in Kaposi's sarcoma tissue
    Article Snippet: .. 2.2.2 Quantitative real-time reverse transcription PCR (qRT-PCR) After 48 hours transfection, total RNA was extracted from cells using an RNeasy Mini Kit (Qiagen). .. The cDNA was reverse transcribed with an miRcute miRNA cDNA Kit (Tiangen, Beijing, China) and a PrimeScript First strand cDNA Synthesis Kit (TaKaRa, Dalian, China) following the manufacturer's instructions.

    In Vitro:

    Article Title: A Novel Set of Cas9 Fusion Proteins to stimulate Homologous Recombination: Cas9-HRs
    Article Snippet: Purification protocols were based on a modified version of a previously published two-step Cas9 purification protocol . .. Cas9-HR and Cas9 in-vitro exonuclease assay DNA was isolated from wildtype K562 cells, and amplified using standard Taq DNA polymerase and HBB-out-F (5’-aacgatcctgagacttccaca-3’) and HBB-out-R (5’-tgcttaccaagctgtgattcc-3’), Tm=56 for 35 cycles, and purified using the Quiagen PCR cleanup kit. .. Cas9-HR 3,4,8 or Cas9 were combined with the amplified HBB fragment at a 10:1 molar ratio (30nM: 3nM) in 1X Cas9 reaction Buffer (50mM Tris, 100mM NaCl, 10mM MgCl2, 1mM DTT, pH7.9) and incubated for 1hr at 37°C, after which 1µL of Proteinase K (NEB) was added and the reaction was incubated for an additional 20 minutes at 65°C.

    Article Title: The short-chain fatty acid pentanoate suppresses autoimmunity by modulating the metabolic-epigenetic crosstalk in lymphocytes
    Article Snippet: For the measurement of glycolytic capacity, the difference between ECAR values following oligomycin injection and baseline ECAR reading was used. .. RNA-seq analysis RNA was purified from in vitro generated Bregs or Th17 cells using the RNeasy Mini kit (Qiagen). .. The purified RNAs were sequenced on an Illumina HiSeq 1500 device.

    Isolation:

    Article Title: A Novel Set of Cas9 Fusion Proteins to stimulate Homologous Recombination: Cas9-HRs
    Article Snippet: Purification protocols were based on a modified version of a previously published two-step Cas9 purification protocol . .. Cas9-HR and Cas9 in-vitro exonuclease assay DNA was isolated from wildtype K562 cells, and amplified using standard Taq DNA polymerase and HBB-out-F (5’-aacgatcctgagacttccaca-3’) and HBB-out-R (5’-tgcttaccaagctgtgattcc-3’), Tm=56 for 35 cycles, and purified using the Quiagen PCR cleanup kit. .. Cas9-HR 3,4,8 or Cas9 were combined with the amplified HBB fragment at a 10:1 molar ratio (30nM: 3nM) in 1X Cas9 reaction Buffer (50mM Tris, 100mM NaCl, 10mM MgCl2, 1mM DTT, pH7.9) and incubated for 1hr at 37°C, after which 1µL of Proteinase K (NEB) was added and the reaction was incubated for an additional 20 minutes at 65°C.

    Article Title: Biliary tract instillation of a SMAC mimetic induces TRAIL-dependent acute sclerosing cholangitis-like injury in mice
    Article Snippet: Serum analysis Serum alanine aminotransferase (ALT), alkaline phosphatase, total bile acids and total bilirubin were measured using a commercially available veterinary chemistry analyzer (VetScan 2, Abaxis, Union City, CA, USA). .. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) Total RNA was isolated from cell cultures and frozen liver tissue using RNeasy Plus mini kit (Qiagen, Hilden, Germany). .. Reverse transcription was performed using Moloney murine leukemia virus reverse transcriptase and random primers (both Invitrogen, Carlsbad, CA, USA).

    Amplification:

    Article Title: A Novel Set of Cas9 Fusion Proteins to stimulate Homologous Recombination: Cas9-HRs
    Article Snippet: Purification protocols were based on a modified version of a previously published two-step Cas9 purification protocol . .. Cas9-HR and Cas9 in-vitro exonuclease assay DNA was isolated from wildtype K562 cells, and amplified using standard Taq DNA polymerase and HBB-out-F (5’-aacgatcctgagacttccaca-3’) and HBB-out-R (5’-tgcttaccaagctgtgattcc-3’), Tm=56 for 35 cycles, and purified using the Quiagen PCR cleanup kit. .. Cas9-HR 3,4,8 or Cas9 were combined with the amplified HBB fragment at a 10:1 molar ratio (30nM: 3nM) in 1X Cas9 reaction Buffer (50mM Tris, 100mM NaCl, 10mM MgCl2, 1mM DTT, pH7.9) and incubated for 1hr at 37°C, after which 1µL of Proteinase K (NEB) was added and the reaction was incubated for an additional 20 minutes at 65°C.

    Purification:

    Article Title: A Novel Set of Cas9 Fusion Proteins to stimulate Homologous Recombination: Cas9-HRs
    Article Snippet: Purification protocols were based on a modified version of a previously published two-step Cas9 purification protocol . .. Cas9-HR and Cas9 in-vitro exonuclease assay DNA was isolated from wildtype K562 cells, and amplified using standard Taq DNA polymerase and HBB-out-F (5’-aacgatcctgagacttccaca-3’) and HBB-out-R (5’-tgcttaccaagctgtgattcc-3’), Tm=56 for 35 cycles, and purified using the Quiagen PCR cleanup kit. .. Cas9-HR 3,4,8 or Cas9 were combined with the amplified HBB fragment at a 10:1 molar ratio (30nM: 3nM) in 1X Cas9 reaction Buffer (50mM Tris, 100mM NaCl, 10mM MgCl2, 1mM DTT, pH7.9) and incubated for 1hr at 37°C, after which 1µL of Proteinase K (NEB) was added and the reaction was incubated for an additional 20 minutes at 65°C.

    Article Title: The short-chain fatty acid pentanoate suppresses autoimmunity by modulating the metabolic-epigenetic crosstalk in lymphocytes
    Article Snippet: For the measurement of glycolytic capacity, the difference between ECAR values following oligomycin injection and baseline ECAR reading was used. .. RNA-seq analysis RNA was purified from in vitro generated Bregs or Th17 cells using the RNeasy Mini kit (Qiagen). .. The purified RNAs were sequenced on an Illumina HiSeq 1500 device.

    RNA Sequencing Assay:

    Article Title: The short-chain fatty acid pentanoate suppresses autoimmunity by modulating the metabolic-epigenetic crosstalk in lymphocytes
    Article Snippet: For the measurement of glycolytic capacity, the difference between ECAR values following oligomycin injection and baseline ECAR reading was used. .. RNA-seq analysis RNA was purified from in vitro generated Bregs or Th17 cells using the RNeasy Mini kit (Qiagen). .. The purified RNAs were sequenced on an Illumina HiSeq 1500 device.

    Generated:

    Article Title: The short-chain fatty acid pentanoate suppresses autoimmunity by modulating the metabolic-epigenetic crosstalk in lymphocytes
    Article Snippet: For the measurement of glycolytic capacity, the difference between ECAR values following oligomycin injection and baseline ECAR reading was used. .. RNA-seq analysis RNA was purified from in vitro generated Bregs or Th17 cells using the RNeasy Mini kit (Qiagen). .. The purified RNAs were sequenced on an Illumina HiSeq 1500 device.

    Fractionation:

    Article Title: Anti-Psoriatic Drug Monomethylfumarate Increases Nuclear Factor Erythroid 2-Related Factor 2 Levels and Induces Aquaporin-3 mRNA and Protein Expression
    Article Snippet: .. To extract plasma membrane from keratinocytes, we used a Qproteome plasma membrane protein fractionation kit (QIAGEN). .. Near-confluent keratinocytes were treated with 0 and 750 μ M MMF for 24 hours, after which membrane fractionation was performed according to the manufacturer’s instructions.

    Agarose Gel Electrophoresis:

    Article Title: Development of Genomic Array Footprinting for Identification of Conditionally Essential Genes in Streptococcus pneumoniae ▿ ▿ †
    Article Snippet: Ligated DNA was used as a template in two consecutive PCRs with Taq DNA polymerase: the first reaction contained primers LinkAmp1 (Table ) and PBMrTn5 (Table ), and the second contained nested PCR primers LinkAmp2 (Table ) and PBMrTn3 (Table ). .. PCR cycling conditions for both reactions were as follows: 94°C for 4 min; 20 cycles of 94°C for 30 s, 63°C for 1 min, and 72°C for 1.5 min; and 72°C for 3 min. PCR products were loaded on a 2% agarose gel, and DNA fragments with the preferred size were excised and extracted with a Qiaquick gel purification kit (QIAGEN). .. In vitro transcription reaction and subsequent DNase I digestion were performed with a T7 MEGAscript kit (Ambion).

    Gel Purification:

    Article Title: Development of Genomic Array Footprinting for Identification of Conditionally Essential Genes in Streptococcus pneumoniae ▿ ▿ †
    Article Snippet: Ligated DNA was used as a template in two consecutive PCRs with Taq DNA polymerase: the first reaction contained primers LinkAmp1 (Table ) and PBMrTn5 (Table ), and the second contained nested PCR primers LinkAmp2 (Table ) and PBMrTn3 (Table ). .. PCR cycling conditions for both reactions were as follows: 94°C for 4 min; 20 cycles of 94°C for 30 s, 63°C for 1 min, and 72°C for 1.5 min; and 72°C for 3 min. PCR products were loaded on a 2% agarose gel, and DNA fragments with the preferred size were excised and extracted with a Qiaquick gel purification kit (QIAGEN). .. In vitro transcription reaction and subsequent DNase I digestion were performed with a T7 MEGAscript kit (Ambion).

    Quantitative RT-PCR:

    Article Title: Biliary tract instillation of a SMAC mimetic induces TRAIL-dependent acute sclerosing cholangitis-like injury in mice
    Article Snippet: Serum analysis Serum alanine aminotransferase (ALT), alkaline phosphatase, total bile acids and total bilirubin were measured using a commercially available veterinary chemistry analyzer (VetScan 2, Abaxis, Union City, CA, USA). .. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) Total RNA was isolated from cell cultures and frozen liver tissue using RNeasy Plus mini kit (Qiagen, Hilden, Germany). .. Reverse transcription was performed using Moloney murine leukemia virus reverse transcriptase and random primers (both Invitrogen, Carlsbad, CA, USA).

    Article Title: MicroRNA-126 regulates the phosphatidylinositol-3 kinase (PI3K)/protein kinase B (AKT) pathway in SLK cells in vitro and the expression of its pathway members in Kaposi's sarcoma tissue
    Article Snippet: .. 2.2.2 Quantitative real-time reverse transcription PCR (qRT-PCR) After 48 hours transfection, total RNA was extracted from cells using an RNeasy Mini Kit (Qiagen). .. The cDNA was reverse transcribed with an miRcute miRNA cDNA Kit (Tiangen, Beijing, China) and a PrimeScript First strand cDNA Synthesis Kit (TaKaRa, Dalian, China) following the manufacturer's instructions.

    Transfection:

    Article Title: MicroRNA-126 regulates the phosphatidylinositol-3 kinase (PI3K)/protein kinase B (AKT) pathway in SLK cells in vitro and the expression of its pathway members in Kaposi's sarcoma tissue
    Article Snippet: .. 2.2.2 Quantitative real-time reverse transcription PCR (qRT-PCR) After 48 hours transfection, total RNA was extracted from cells using an RNeasy Mini Kit (Qiagen). .. The cDNA was reverse transcribed with an miRcute miRNA cDNA Kit (Tiangen, Beijing, China) and a PrimeScript First strand cDNA Synthesis Kit (TaKaRa, Dalian, China) following the manufacturer's instructions.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Qiagen dna fragments
    Phylogenetic tree of SRB based on dsr B amino acid sequences obtained by <t>PCR-DGGE</t> and in relation to cultured SRB and the closest uncultured relatives. The dsr B sequences from <t>DNA</t> and RNA (directly obtained from untreated fracture fluid) and substrate induced samples are represented in different colours, DNA—red, RNA—light red, CH 3 OH—light blue, CH 3 OH + SO 4 2− —blue, CH 4 —light green and CH 4 + SO 4 2− —green. The band number indicates the DGGE band number in Fig. S1. Nonparametric bootstrap values are shown at nodes found in > 50% of 1000 pseudoreplicates. The scale bar indicates 0.08 amino acid substitutions. The tree is rooted by Archaeglobus veneficus.
    Dna Fragments, supplied by Qiagen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna fragments/product/Qiagen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna fragments - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    Image Search Results


    Phylogenetic tree of SRB based on dsr B amino acid sequences obtained by PCR-DGGE and in relation to cultured SRB and the closest uncultured relatives. The dsr B sequences from DNA and RNA (directly obtained from untreated fracture fluid) and substrate induced samples are represented in different colours, DNA—red, RNA—light red, CH 3 OH—light blue, CH 3 OH + SO 4 2− —blue, CH 4 —light green and CH 4 + SO 4 2− —green. The band number indicates the DGGE band number in Fig. S1. Nonparametric bootstrap values are shown at nodes found in > 50% of 1000 pseudoreplicates. The scale bar indicates 0.08 amino acid substitutions. The tree is rooted by Archaeglobus veneficus.

    Journal: Microorganisms

    Article Title: Rapid Reactivation of Deep Subsurface Microbes in the Presence of C-1 Compounds

    doi: 10.3390/microorganisms3010017

    Figure Lengend Snippet: Phylogenetic tree of SRB based on dsr B amino acid sequences obtained by PCR-DGGE and in relation to cultured SRB and the closest uncultured relatives. The dsr B sequences from DNA and RNA (directly obtained from untreated fracture fluid) and substrate induced samples are represented in different colours, DNA—red, RNA—light red, CH 3 OH—light blue, CH 3 OH + SO 4 2− —blue, CH 4 —light green and CH 4 + SO 4 2− —green. The band number indicates the DGGE band number in Fig. S1. Nonparametric bootstrap values are shown at nodes found in > 50% of 1000 pseudoreplicates. The scale bar indicates 0.08 amino acid substitutions. The tree is rooted by Archaeglobus veneficus.

    Article Snippet: Methanotroph and NRB Diversity According to Clone Libraries The DNA fragments from pmo A- and nar G-targeted PCR were excised from the agarose gel and purified with the Qiaquick Gel Extraction kit (Qiagen, Hilden, Germany).

    Techniques: Sulforhodamine B Assay, Polymerase Chain Reaction, Denaturing Gradient Gel Electrophoresis, Cell Culture

    PCR and schematic nucleotide sequence alignments of the control region (CR) of Diaphorina citri . ( a ) Multiple amplicons of PCR of CR of individual D. citri from California, Florida and Guangdong-China based on primer set CR-f/CR-r. M, DNA Marker (2,000 bp); CApsy, FLpsy and GDpsy, D. citri from California, Florida and Guangdong-China, respectively, used for mitogenome sequencing; CA1-6: D. citri individuals from southern California, USA; FL1-6: D. citri individuals from Immokalee, Florida, USA; GD1-6: D. citri individuals from Guangzhou, Guangdong, China. BC, Bactericera cockerelli , showing a single amplicon from its CR as published previously 24 . ( b ) Schematic nucleotide sequence alignments of four variable amplicon clones from CR in a D. citri individual from California. rrnS = small ribosomal RNA.

    Journal: Scientific Reports

    Article Title: Analyses of Mitogenome Sequences Revealed that Asian Citrus Psyllids (Diaphorina citri) from California Were Related to Those from Florida

    doi: 10.1038/s41598-017-10713-3

    Figure Lengend Snippet: PCR and schematic nucleotide sequence alignments of the control region (CR) of Diaphorina citri . ( a ) Multiple amplicons of PCR of CR of individual D. citri from California, Florida and Guangdong-China based on primer set CR-f/CR-r. M, DNA Marker (2,000 bp); CApsy, FLpsy and GDpsy, D. citri from California, Florida and Guangdong-China, respectively, used for mitogenome sequencing; CA1-6: D. citri individuals from southern California, USA; FL1-6: D. citri individuals from Immokalee, Florida, USA; GD1-6: D. citri individuals from Guangzhou, Guangdong, China. BC, Bactericera cockerelli , showing a single amplicon from its CR as published previously 24 . ( b ) Schematic nucleotide sequence alignments of four variable amplicon clones from CR in a D. citri individual from California. rrnS = small ribosomal RNA.

    Article Snippet: The amplified DNA fragments were cut by NucleoSpin® Gel and PCR Clean-up kit (QIAGEN, Valencia, USA).

    Techniques: Polymerase Chain Reaction, Sequencing, Marker, Amplification, Clone Assay

    DNA isolation efficiency using the Norgen Stool DNA Isolation Kit. Across all samples (ranging from 800 to 8 ng of digested human gDNA spiked into stool slurries and carried through DNA isolation), the recovery of the spike-ins averaged 62%. Error bars represent standard deviations.

    Journal: Scientific Reports

    Article Title: A Pipeline for Faecal Host DNA Analysis by Absolute Quantification of LINE-1 and Mitochondrial Genomic Elements Using ddPCR

    doi: 10.1038/s41598-019-41753-6

    Figure Lengend Snippet: DNA isolation efficiency using the Norgen Stool DNA Isolation Kit. Across all samples (ranging from 800 to 8 ng of digested human gDNA spiked into stool slurries and carried through DNA isolation), the recovery of the spike-ins averaged 62%. Error bars represent standard deviations.

    Article Snippet: At the 10 μg DNA input level, the Norgen reagents showed the most uniform and highest recovery of DNA fragments, Zymo was comparable in recovery across the DNA ladder range except it showed diminished recovery of the 100 bp fragment (see red arrow in Fig. ), and the Qiagen kit showed the lowest recovery of DNA across the entire size range.

    Techniques: DNA Extraction

    Comparison of DNA recovery of a ladder consisting of fragments as short as 100 bp using the Zymo Quick-DNA faecal/Soil Microbe Miniprep Kit (Zymo), the Norgen Stool DNA Isolation Kit (Norgen), and the QIAamp DNA Stool Mini Kit (Qiagen). ( a ) For direct visualization of DNA recovery from the three different purification processes, DNA ladder was either loaded d irectly onto TapeStation (D) or was subjected to one of the three p urification protocols before being loaded onto TapeStation (P). Please note that band intensities between lanes D1 and D2 are not directly comparable because the higher input into D1 lanes may be beyond the linear range of the assay. In a 100% recovery scenario, the D and P lanes should contain the same amount of DNA. ( b ) A representative image of gel electrophoresis on TapeStation. The size marker lane was cropped out for clarity (the uncropped, full-length gel is presented in Supplementary Fig. S1 ). The purple and green lines in each lane represent internal upper and lower markers, respectively, for sizing and alignment. The red arrow denotes low recovery of the 100 bp band from the Zymo-extracted ladder samples.

    Journal: Scientific Reports

    Article Title: A Pipeline for Faecal Host DNA Analysis by Absolute Quantification of LINE-1 and Mitochondrial Genomic Elements Using ddPCR

    doi: 10.1038/s41598-019-41753-6

    Figure Lengend Snippet: Comparison of DNA recovery of a ladder consisting of fragments as short as 100 bp using the Zymo Quick-DNA faecal/Soil Microbe Miniprep Kit (Zymo), the Norgen Stool DNA Isolation Kit (Norgen), and the QIAamp DNA Stool Mini Kit (Qiagen). ( a ) For direct visualization of DNA recovery from the three different purification processes, DNA ladder was either loaded d irectly onto TapeStation (D) or was subjected to one of the three p urification protocols before being loaded onto TapeStation (P). Please note that band intensities between lanes D1 and D2 are not directly comparable because the higher input into D1 lanes may be beyond the linear range of the assay. In a 100% recovery scenario, the D and P lanes should contain the same amount of DNA. ( b ) A representative image of gel electrophoresis on TapeStation. The size marker lane was cropped out for clarity (the uncropped, full-length gel is presented in Supplementary Fig. S1 ). The purple and green lines in each lane represent internal upper and lower markers, respectively, for sizing and alignment. The red arrow denotes low recovery of the 100 bp band from the Zymo-extracted ladder samples.

    Article Snippet: At the 10 μg DNA input level, the Norgen reagents showed the most uniform and highest recovery of DNA fragments, Zymo was comparable in recovery across the DNA ladder range except it showed diminished recovery of the 100 bp fragment (see red arrow in Fig. ), and the Qiagen kit showed the lowest recovery of DNA across the entire size range.

    Techniques: DNA Extraction, Purification, Nucleic Acid Electrophoresis, Marker

    Confirmation of transformation of Thermoanaerobacter cells using PCR analysis and plasmid rescue experiments. A. PCR amplication of the kanamycin resistance marker-gene from pIKM2. The PCR products were amplified from DNA preparations of pIKM2-transformed X514 cells by sonoporation (sono-) or electroporation (electro-). The plasmid pIKM2 and wild-type X514 DNA were used as PCR template for positive (P) and negative (N) controls, respectively. B. Plasmid-pIKM2 rescue experiments. The plasmid DNA were extracted from X514-pIKM2 transformants, and “rescued” in E. coli (see Methods ). The E. coli -rescued plasmids were digested by XbaI /SmaI and separated on 1% agarose gel. Plasmid pIKM2 was used as positive control (P). C. Plasmid-pHL015 rescue experiments. The plasmid DNA were extracted from X514-pHL015 transformants, and rescued in E. coli . The E. coli cells harboring rescued plasmids were visualized under Olympus-BX51 microscope equipped with a CCD. Scale bar represents 2 mm. E. coli /pHL015-S: the E.coli -rescued plasmid that was transformed into X514 by sonoporation. E. coli /pHL015-E: the E.coli -rescued plasmid that was transformed into X514 by electroporation. “M”: molecular weight marker.

    Journal: PLoS ONE

    Article Title: Ultrasound-Mediated DNA Transformation in Thermophilic Gram-Positive Anaerobes

    doi: 10.1371/journal.pone.0012582

    Figure Lengend Snippet: Confirmation of transformation of Thermoanaerobacter cells using PCR analysis and plasmid rescue experiments. A. PCR amplication of the kanamycin resistance marker-gene from pIKM2. The PCR products were amplified from DNA preparations of pIKM2-transformed X514 cells by sonoporation (sono-) or electroporation (electro-). The plasmid pIKM2 and wild-type X514 DNA were used as PCR template for positive (P) and negative (N) controls, respectively. B. Plasmid-pIKM2 rescue experiments. The plasmid DNA were extracted from X514-pIKM2 transformants, and “rescued” in E. coli (see Methods ). The E. coli -rescued plasmids were digested by XbaI /SmaI and separated on 1% agarose gel. Plasmid pIKM2 was used as positive control (P). C. Plasmid-pHL015 rescue experiments. The plasmid DNA were extracted from X514-pHL015 transformants, and rescued in E. coli . The E. coli cells harboring rescued plasmids were visualized under Olympus-BX51 microscope equipped with a CCD. Scale bar represents 2 mm. E. coli /pHL015-S: the E.coli -rescued plasmid that was transformed into X514 by sonoporation. E. coli /pHL015-E: the E.coli -rescued plasmid that was transformed into X514 by electroporation. “M”: molecular weight marker.

    Article Snippet: Methylated DNA fragments were purified by the QIAquick PCR purification kit (Qiagen).

    Techniques: Transformation Assay, Polymerase Chain Reaction, Plasmid Preparation, Marker, Amplification, Electroporation, Agarose Gel Electrophoresis, Positive Control, Microscopy, Molecular Weight