dna fragments  (New England Biolabs)


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    Structured Review

    New England Biolabs dna fragments
    Dna Fragments, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 653 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna fragments/product/New England Biolabs
    Average 95 stars, based on 653 article reviews
    Price from $9.99 to $1999.99
    dna fragments - by Bioz Stars, 2020-01
    95/100 stars

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    Related Articles

    Multiplexing:

    Article Title: AFSM sequencing approach: a simple and rapid method for genome-wide SNP and methylation site discovery and genetic mapping
    Article Snippet: Paragraph title: Multiplexing, Amplification and Sequencing ... Restriction fragments from each library were then amplified in 50 μL volumes containing 10 μL of pooled DNA fragments, NEB 2x Taq Master Mix (New England Biolabs), and 20 μM each of the primer pairs.

    Amplification:

    Article Title: AFSM sequencing approach: a simple and rapid method for genome-wide SNP and methylation site discovery and genetic mapping
    Article Snippet: .. Restriction fragments from each library were then amplified in 50 μL volumes containing 10 μL of pooled DNA fragments, NEB 2x Taq Master Mix (New England Biolabs), and 20 μM each of the primer pairs. ..

    Article Title: Epigenomic and functional analyses reveal roles of epialleles in the loss of photoperiod sensitivity during domestication of allotetraploid cottons
    Article Snippet: End repair (NEBNext® End Repair Module) was performed on the DNA fragments followed by adding an ”A” base to the 3′ end (NEBNext® dA-Tailing Module), and the resulting DNA fragments were ligated to the methylated DNA adapter (NEXTflex™ DNA Barcodes, Bioo Scientific, Austin, TX, USA). .. The bisulfite-converted DNA was amplified by 12 cycles of PCR using LongAmp® Taq DNA Polymerase (NEB) and subject to purification using AMPure beads (Beckman Coulter).

    Article Title: Genome-wide differential mRNA expression profiles in follicles of two breeds and at two stages of estrus cycle of gilts
    Article Snippet: After adding buffer, dNTPs, DNA polymerase I (New England Biolabs) and RNase H (Invitrogen), the second strand cDNA synthesis was subsequently performed using DNA polymerase I and RNase H. After adenylation of 3’ ends of DNA fragments, NEBNext Adapter was ligated to prepare for hybridization. .. The cDNA fragments with 150–200 bp in length were selected for PCR amplification to create cDNA libraries.

    Agarose Gel Electrophoresis:

    Article Title: AFSM sequencing approach: a simple and rapid method for genome-wide SNP and methylation site discovery and genetic mapping
    Article Snippet: Restriction fragments from each library were then amplified in 50 μL volumes containing 10 μL of pooled DNA fragments, NEB 2x Taq Master Mix (New England Biolabs), and 20 μM each of the primer pairs. .. Restriction fragments from each library were then amplified in 50 μL volumes containing 10 μL of pooled DNA fragments, NEB 2x Taq Master Mix (New England Biolabs), and 20 μM each of the primer pairs.

    BAC Assay:

    Article Title: Incorporation of a lambda phage recombination system and EGFP detection to simplify mutagenesis of Herpes simplex virus bacterial artificial chromosomes
    Article Snippet: BAC DNA was isolated from a large culture (1.5 liters) using Qiagen's Large-Construct kit. .. Restriction enzymes, calf intestinal phosphatase (CIP), and Klenow fragment used to blunt DNA fragments were obtained from NEB (Beverly, MA).

    Magnetic Beads:

    Article Title: Genome-wide differential mRNA expression profiles in follicles of two breeds and at two stages of estrus cycle of gilts
    Article Snippet: Amount of 3 μg RNA per sample was used to purify mRNA using poly-T oligo attached magnetic beads and then the purified mRNA was interrupted into short fragments (about 200 bp) by the fragmentation buffer. .. After adding buffer, dNTPs, DNA polymerase I (New England Biolabs) and RNase H (Invitrogen), the second strand cDNA synthesis was subsequently performed using DNA polymerase I and RNase H. After adenylation of 3’ ends of DNA fragments, NEBNext Adapter was ligated to prepare for hybridization.

    Isolation:

    Article Title: Incorporation of a lambda phage recombination system and EGFP detection to simplify mutagenesis of Herpes simplex virus bacterial artificial chromosomes
    Article Snippet: BAC DNA was isolated from a large culture (1.5 liters) using Qiagen's Large-Construct kit. .. Restriction enzymes, calf intestinal phosphatase (CIP), and Klenow fragment used to blunt DNA fragments were obtained from NEB (Beverly, MA).

    Article Title: Identification of the Spinal Expression Profile of Non-coding RNAs Involved in Neuropathic Pain Following Spared Nerve Injury by Sequence Analysis
    Article Snippet: Paragraph title: RNA isolation and RNA quantification and qualification ... After adenylation of 3′ ends of DNA fragments, NEBNext, Adaptor with hairpin loop structure were ligated to prepare for hybridization.

    Synthesized:

    Article Title: Identification of the Spinal Expression Profile of Non-coding RNAs Involved in Neuropathic Pain Following Spared Nerve Injury by Sequence Analysis
    Article Snippet: First strand cDNA was synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNaseH-). .. After adenylation of 3′ ends of DNA fragments, NEBNext, Adaptor with hairpin loop structure were ligated to prepare for hybridization.

    Article Title: Genome-wide differential mRNA expression profiles in follicles of two breeds and at two stages of estrus cycle of gilts
    Article Snippet: First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (RNase H). .. After adding buffer, dNTPs, DNA polymerase I (New England Biolabs) and RNase H (Invitrogen), the second strand cDNA synthesis was subsequently performed using DNA polymerase I and RNase H. After adenylation of 3’ ends of DNA fragments, NEBNext Adapter was ligated to prepare for hybridization.

    Random Hexamer Labeling:

    Article Title: Identification of the Spinal Expression Profile of Non-coding RNAs Involved in Neuropathic Pain Following Spared Nerve Injury by Sequence Analysis
    Article Snippet: First strand cDNA was synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNaseH-). .. After adenylation of 3′ ends of DNA fragments, NEBNext, Adaptor with hairpin loop structure were ligated to prepare for hybridization.

    Article Title: Genome-wide differential mRNA expression profiles in follicles of two breeds and at two stages of estrus cycle of gilts
    Article Snippet: First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (RNase H). .. After adding buffer, dNTPs, DNA polymerase I (New England Biolabs) and RNase H (Invitrogen), the second strand cDNA synthesis was subsequently performed using DNA polymerase I and RNase H. After adenylation of 3’ ends of DNA fragments, NEBNext Adapter was ligated to prepare for hybridization.

    Construct:

    Article Title: Incorporation of a lambda phage recombination system and EGFP detection to simplify mutagenesis of Herpes simplex virus bacterial artificial chromosomes
    Article Snippet: The authenticity of all constructs throughout this study was confirmed by sequencing using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA). .. Restriction enzymes, calf intestinal phosphatase (CIP), and Klenow fragment used to blunt DNA fragments were obtained from NEB (Beverly, MA).

    Article Title: Epigenomic and functional analyses reveal roles of epialleles in the loss of photoperiod sensitivity during domestication of allotetraploid cottons
    Article Snippet: End repair (NEBNext® End Repair Module) was performed on the DNA fragments followed by adding an ”A” base to the 3′ end (NEBNext® dA-Tailing Module), and the resulting DNA fragments were ligated to the methylated DNA adapter (NEXTflex™ DNA Barcodes, Bioo Scientific, Austin, TX, USA). .. For each genotype, MethylC-seq libraries were constructed with two biological replicates and paired-end sequenced for 126 cycles.

    Purification:

    Article Title: AFSM sequencing approach: a simple and rapid method for genome-wide SNP and methylation site discovery and genetic mapping
    Article Snippet: Multiplexing, Amplification and Sequencing One set of 85 EcoRI-HpaII ligated samples were pooled and purified in one tube, and another set of 85 EcoRI-MspI ligated samples were pooled and purified in another tube using an E.Z.N.A. .. Restriction fragments from each library were then amplified in 50 μL volumes containing 10 μL of pooled DNA fragments, NEB 2x Taq Master Mix (New England Biolabs), and 20 μM each of the primer pairs.

    Article Title: Identification of the Spinal Expression Profile of Non-coding RNAs Involved in Neuropathic Pain Following Spared Nerve Injury by Sequence Analysis
    Article Snippet: After adenylation of 3′ ends of DNA fragments, NEBNext, Adaptor with hairpin loop structure were ligated to prepare for hybridization. .. To select cDNA fragments of preferentially 150–200 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA).

    Article Title: Epigenomic and functional analyses reveal roles of epialleles in the loss of photoperiod sensitivity during domestication of allotetraploid cottons
    Article Snippet: End repair (NEBNext® End Repair Module) was performed on the DNA fragments followed by adding an ”A” base to the 3′ end (NEBNext® dA-Tailing Module), and the resulting DNA fragments were ligated to the methylated DNA adapter (NEXTflex™ DNA Barcodes, Bioo Scientific, Austin, TX, USA). .. The adapter-ligated DNA of 200–400 bp was purified using AMPure beads (Beckman Coulter, Brea, CA, USA), followed by sodium bisulfite conversion using the MethylCode™ Bisulfite Conversion Kit (Life Technologies, Foster City, CA, USA).

    Article Title: Genome-wide differential mRNA expression profiles in follicles of two breeds and at two stages of estrus cycle of gilts
    Article Snippet: Amount of 3 μg RNA per sample was used to purify mRNA using poly-T oligo attached magnetic beads and then the purified mRNA was interrupted into short fragments (about 200 bp) by the fragmentation buffer. .. After adding buffer, dNTPs, DNA polymerase I (New England Biolabs) and RNase H (Invitrogen), the second strand cDNA synthesis was subsequently performed using DNA polymerase I and RNase H. After adenylation of 3’ ends of DNA fragments, NEBNext Adapter was ligated to prepare for hybridization.

    Sequencing:

    Article Title: AFSM sequencing approach: a simple and rapid method for genome-wide SNP and methylation site discovery and genetic mapping
    Article Snippet: Paragraph title: Multiplexing, Amplification and Sequencing ... Restriction fragments from each library were then amplified in 50 μL volumes containing 10 μL of pooled DNA fragments, NEB 2x Taq Master Mix (New England Biolabs), and 20 μM each of the primer pairs.

    Article Title: Incorporation of a lambda phage recombination system and EGFP detection to simplify mutagenesis of Herpes simplex virus bacterial artificial chromosomes
    Article Snippet: The authenticity of all constructs throughout this study was confirmed by sequencing using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA). .. Restriction enzymes, calf intestinal phosphatase (CIP), and Klenow fragment used to blunt DNA fragments were obtained from NEB (Beverly, MA).

    Article Title: Identification of the Spinal Expression Profile of Non-coding RNAs Involved in Neuropathic Pain Following Spared Nerve Injury by Sequence Analysis
    Article Snippet: Subsequently, sequencing libraries were generated using the rRNA-depleted RNA by NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations. .. After adenylation of 3′ ends of DNA fragments, NEBNext, Adaptor with hairpin loop structure were ligated to prepare for hybridization.

    Article Title: Genome-wide differential mRNA expression profiles in follicles of two breeds and at two stages of estrus cycle of gilts
    Article Snippet: Library preparation for RNA-seq According to the manufacturer’s manual, sequencing libraries were generated using NEBNext Ultra™ RNA Library Prep Kit for Illumina (NEB, USA). .. After adding buffer, dNTPs, DNA polymerase I (New England Biolabs) and RNase H (Invitrogen), the second strand cDNA synthesis was subsequently performed using DNA polymerase I and RNase H. After adenylation of 3’ ends of DNA fragments, NEBNext Adapter was ligated to prepare for hybridization.

    Generated:

    Article Title: Identification of the Spinal Expression Profile of Non-coding RNAs Involved in Neuropathic Pain Following Spared Nerve Injury by Sequence Analysis
    Article Snippet: Subsequently, sequencing libraries were generated using the rRNA-depleted RNA by NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations. .. After adenylation of 3′ ends of DNA fragments, NEBNext, Adaptor with hairpin loop structure were ligated to prepare for hybridization.

    Article Title: Genome-wide differential mRNA expression profiles in follicles of two breeds and at two stages of estrus cycle of gilts
    Article Snippet: Library preparation for RNA-seq According to the manufacturer’s manual, sequencing libraries were generated using NEBNext Ultra™ RNA Library Prep Kit for Illumina (NEB, USA). .. After adding buffer, dNTPs, DNA polymerase I (New England Biolabs) and RNase H (Invitrogen), the second strand cDNA synthesis was subsequently performed using DNA polymerase I and RNase H. After adenylation of 3’ ends of DNA fragments, NEBNext Adapter was ligated to prepare for hybridization.

    Methylation:

    Article Title: Epigenomic and functional analyses reveal roles of epialleles in the loss of photoperiod sensitivity during domestication of allotetraploid cottons
    Article Snippet: .. End repair (NEBNext® End Repair Module) was performed on the DNA fragments followed by adding an ”A” base to the 3′ end (NEBNext® dA-Tailing Module), and the resulting DNA fragments were ligated to the methylated DNA adapter (NEXTflex™ DNA Barcodes, Bioo Scientific, Austin, TX, USA). .. The adapter-ligated DNA of 200–400 bp was purified using AMPure beads (Beckman Coulter, Brea, CA, USA), followed by sodium bisulfite conversion using the MethylCode™ Bisulfite Conversion Kit (Life Technologies, Foster City, CA, USA).

    Polymerase Chain Reaction:

    Article Title: Identification of the Spinal Expression Profile of Non-coding RNAs Involved in Neuropathic Pain Following Spared Nerve Injury by Sequence Analysis
    Article Snippet: After adenylation of 3′ ends of DNA fragments, NEBNext, Adaptor with hairpin loop structure were ligated to prepare for hybridization. .. Then, 3 μl USER Enzyme (NEB, USA) was used with size-selected, adaptor-ligated cDNA at 37°C for 15 min followed by 5 min at 95°C before PCR.

    Article Title: Epigenomic and functional analyses reveal roles of epialleles in the loss of photoperiod sensitivity during domestication of allotetraploid cottons
    Article Snippet: End repair (NEBNext® End Repair Module) was performed on the DNA fragments followed by adding an ”A” base to the 3′ end (NEBNext® dA-Tailing Module), and the resulting DNA fragments were ligated to the methylated DNA adapter (NEXTflex™ DNA Barcodes, Bioo Scientific, Austin, TX, USA). .. The bisulfite-converted DNA was amplified by 12 cycles of PCR using LongAmp® Taq DNA Polymerase (NEB) and subject to purification using AMPure beads (Beckman Coulter).

    Article Title: Genome-wide differential mRNA expression profiles in follicles of two breeds and at two stages of estrus cycle of gilts
    Article Snippet: After adding buffer, dNTPs, DNA polymerase I (New England Biolabs) and RNase H (Invitrogen), the second strand cDNA synthesis was subsequently performed using DNA polymerase I and RNase H. After adenylation of 3’ ends of DNA fragments, NEBNext Adapter was ligated to prepare for hybridization. .. The cDNA fragments with 150–200 bp in length were selected for PCR amplification to create cDNA libraries.

    RNA Sequencing Assay:

    Article Title: Genome-wide differential mRNA expression profiles in follicles of two breeds and at two stages of estrus cycle of gilts
    Article Snippet: Paragraph title: Library preparation for RNA-seq ... After adding buffer, dNTPs, DNA polymerase I (New England Biolabs) and RNase H (Invitrogen), the second strand cDNA synthesis was subsequently performed using DNA polymerase I and RNase H. After adenylation of 3’ ends of DNA fragments, NEBNext Adapter was ligated to prepare for hybridization.

    Chick Chorioallantoic Membrane Assay:

    Article Title: Incorporation of a lambda phage recombination system and EGFP detection to simplify mutagenesis of Herpes simplex virus bacterial artificial chromosomes
    Article Snippet: Restriction enzymes, calf intestinal phosphatase (CIP), and Klenow fragment used to blunt DNA fragments were obtained from NEB (Beverly, MA). .. Antibiotics and supplements in solid agar plates and liquid media were used at the following concentrations: zeocin (Zeo), 25 μg/ml; kanamycin (Kan), 50 μg/ml; chloramphenicol (Cam), 20 μg/ml; Ampicillin (Amp), 100 μg/ml; Sucrose (Suc) 5% (w/v).

    Hybridization:

    Article Title: Identification of the Spinal Expression Profile of Non-coding RNAs Involved in Neuropathic Pain Following Spared Nerve Injury by Sequence Analysis
    Article Snippet: .. After adenylation of 3′ ends of DNA fragments, NEBNext, Adaptor with hairpin loop structure were ligated to prepare for hybridization. .. To select cDNA fragments of preferentially 150–200 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA).

    Article Title: Genome-wide differential mRNA expression profiles in follicles of two breeds and at two stages of estrus cycle of gilts
    Article Snippet: .. After adding buffer, dNTPs, DNA polymerase I (New England Biolabs) and RNase H (Invitrogen), the second strand cDNA synthesis was subsequently performed using DNA polymerase I and RNase H. After adenylation of 3’ ends of DNA fragments, NEBNext Adapter was ligated to prepare for hybridization. .. The cDNA fragments with 150–200 bp in length were selected for PCR amplification to create cDNA libraries.

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