dna fragmentation  (Boehringer Mannheim)

 
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    Boehringer Mannheim dna fragmentation
    Flow cytometric analysis of Ltx-induced <t>DNA</t> fragmentation in HL-60 cells. HL-60 cells were incubated with vector control (pOTS) supernatant (A) for 24 h or with 0.2 Ltx unit for 2, 6, or 24 h (B through D, respectively) and then labeled by the <t>TUNEL</t> reaction. The cells were then analyzed on a flow cytometer, and the data are plotted as the relative amount of DNA fragmentation (log fluorescence) versus relative cell number. In this representative experiment, 27.5% of the cells treated with 0.2 Ltx unit for 24 h were TUNEL positive, while 4.1% of vector control supernatant-treated cells were positive. The frequency of cells exhibiting DNA cleavage increased with time: 16.6 and 18.9% were TUNEL positive following 2- and 6-h exposures to Ltx, respectively. These results are representative of those from three experiments; at least 10,000 cells were analyzed per experiment.
    Dna Fragmentation, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Actinobacillus actinomycetemcomitans Leukotoxin Induces Apoptosis in HL-60 Cells"

    Article Title: Actinobacillus actinomycetemcomitans Leukotoxin Induces Apoptosis in HL-60 Cells

    Journal: Infection and Immunity

    doi:

    Flow cytometric analysis of Ltx-induced DNA fragmentation in HL-60 cells. HL-60 cells were incubated with vector control (pOTS) supernatant (A) for 24 h or with 0.2 Ltx unit for 2, 6, or 24 h (B through D, respectively) and then labeled by the TUNEL reaction. The cells were then analyzed on a flow cytometer, and the data are plotted as the relative amount of DNA fragmentation (log fluorescence) versus relative cell number. In this representative experiment, 27.5% of the cells treated with 0.2 Ltx unit for 24 h were TUNEL positive, while 4.1% of vector control supernatant-treated cells were positive. The frequency of cells exhibiting DNA cleavage increased with time: 16.6 and 18.9% were TUNEL positive following 2- and 6-h exposures to Ltx, respectively. These results are representative of those from three experiments; at least 10,000 cells were analyzed per experiment.
    Figure Legend Snippet: Flow cytometric analysis of Ltx-induced DNA fragmentation in HL-60 cells. HL-60 cells were incubated with vector control (pOTS) supernatant (A) for 24 h or with 0.2 Ltx unit for 2, 6, or 24 h (B through D, respectively) and then labeled by the TUNEL reaction. The cells were then analyzed on a flow cytometer, and the data are plotted as the relative amount of DNA fragmentation (log fluorescence) versus relative cell number. In this representative experiment, 27.5% of the cells treated with 0.2 Ltx unit for 24 h were TUNEL positive, while 4.1% of vector control supernatant-treated cells were positive. The frequency of cells exhibiting DNA cleavage increased with time: 16.6 and 18.9% were TUNEL positive following 2- and 6-h exposures to Ltx, respectively. These results are representative of those from three experiments; at least 10,000 cells were analyzed per experiment.

    Techniques Used: Flow Cytometry, Incubation, Plasmid Preparation, Labeling, TUNEL Assay, Cytometry, Fluorescence

    2) Product Images from "The Aggregatibacter actinomycetemcomitans Cytolethal Distending Toxin Active Subunit CdtB Contains a Cholesterol Recognition Sequence Required for Toxin Binding and Subunit Internalization"

    Article Title: The Aggregatibacter actinomycetemcomitans Cytolethal Distending Toxin Active Subunit CdtB Contains a Cholesterol Recognition Sequence Required for Toxin Binding and Subunit Internalization

    Journal: Infection and Immunity

    doi: 10.1128/IAI.00788-15

    Effect of CdtB CRAC site mutants on DNA fragmentation in Jurkat cells. Jurkat cells were treated with 10 ng/nl each of CdtA and CdtC in the presence of 0 to 20 ng/ml CdtB WT (●), CdtB V104P (▼), CdtB Y105P (▲), CdtB Y107P (■), or CdtB R110P (◆) for 48 h. The cells were then analyzed by flow cytometry for the presence of DNA fragmentation as described in Materials and Methods. Results are plotted as net percent apoptotic cells versus CdtB concentration and represent the means ± SEM of three experiments. *, P
    Figure Legend Snippet: Effect of CdtB CRAC site mutants on DNA fragmentation in Jurkat cells. Jurkat cells were treated with 10 ng/nl each of CdtA and CdtC in the presence of 0 to 20 ng/ml CdtB WT (●), CdtB V104P (▼), CdtB Y105P (▲), CdtB Y107P (■), or CdtB R110P (◆) for 48 h. The cells were then analyzed by flow cytometry for the presence of DNA fragmentation as described in Materials and Methods. Results are plotted as net percent apoptotic cells versus CdtB concentration and represent the means ± SEM of three experiments. *, P

    Techniques Used: Flow Cytometry, Cytometry, Concentration Assay

    3) Product Images from "Nitric Oxide and Apoptosis Induced in Peyer's Patches by Attenuated Strains of Salmonella enterica Serovar Enteritidis"

    Article Title: Nitric Oxide and Apoptosis Induced in Peyer's Patches by Attenuated Strains of Salmonella enterica Serovar Enteritidis

    Journal: Infection and Immunity

    doi:

    Apoptosis in PP of mice immunized with protective and nonprotective mutants of S. enterica serovar Enteritidis. Apoptosis was assessed using a cell death detection ELISA. The y axis represents DNA fragmentation, as measured by the enrichment factor (see Materials and Methods). Mice treated with AG received two intraperitoneal inoculations (1 mg), 24 h and 30 min before immunization. Data are median enrichment factors for six animals. Asterisk indicates significantly different values ( P
    Figure Legend Snippet: Apoptosis in PP of mice immunized with protective and nonprotective mutants of S. enterica serovar Enteritidis. Apoptosis was assessed using a cell death detection ELISA. The y axis represents DNA fragmentation, as measured by the enrichment factor (see Materials and Methods). Mice treated with AG received two intraperitoneal inoculations (1 mg), 24 h and 30 min before immunization. Data are median enrichment factors for six animals. Asterisk indicates significantly different values ( P

    Techniques Used: Mouse Assay, Enzyme-linked Immunosorbent Assay

    4) Product Images from "The Histone Lysine-specific Demethylase 1 Inhibitor, SP2509 Exerts Cytotoxic Effects against Renal Cancer Cells through Downregulation of Bcl-2 and Mcl-1"

    Article Title: The Histone Lysine-specific Demethylase 1 Inhibitor, SP2509 Exerts Cytotoxic Effects against Renal Cancer Cells through Downregulation of Bcl-2 and Mcl-1

    Journal: Journal of Cancer Prevention

    doi: 10.15430/JCP.2020.25.2.79

    LSD1 inhibitor SP2509 induces apoptosis. (A) Caki cells were treated with SP2509 (0.5-2 μM) for 24 hours. The cell morphology and nuclear condensation were examined by using an interference light microscope. White arrows showed nuclear chromatin condensation. (B) The sub-G1 population and protein expression were analyzed by flow cytometry and Western blotting. (C) The fragmentation of the nuclei was determined by using a DNA fragmentation assay kit. (D) Caspase activity was determined by using the caspase DEVDase assay kit. (E) Caki cells were treated with SP2509 (2 μM) in the presence or absence of a pan-caspase inhibitor, z-VAD-fmk (20 μM) for 24 hours. The sub-G1 population and protein expression were analyzed by flow cytometry and Western blotting. The values in graphs represent the mean ± SD of three independent samples. DAPI, 4’, 6’-diamidino-2-phenylindole. a P
    Figure Legend Snippet: LSD1 inhibitor SP2509 induces apoptosis. (A) Caki cells were treated with SP2509 (0.5-2 μM) for 24 hours. The cell morphology and nuclear condensation were examined by using an interference light microscope. White arrows showed nuclear chromatin condensation. (B) The sub-G1 population and protein expression were analyzed by flow cytometry and Western blotting. (C) The fragmentation of the nuclei was determined by using a DNA fragmentation assay kit. (D) Caspase activity was determined by using the caspase DEVDase assay kit. (E) Caki cells were treated with SP2509 (2 μM) in the presence or absence of a pan-caspase inhibitor, z-VAD-fmk (20 μM) for 24 hours. The sub-G1 population and protein expression were analyzed by flow cytometry and Western blotting. The values in graphs represent the mean ± SD of three independent samples. DAPI, 4’, 6’-diamidino-2-phenylindole. a P

    Techniques Used: Light Microscopy, Expressing, Flow Cytometry, Western Blot, DNA Fragmentation Assay, Activity Assay

    5) Product Images from "Internalization of the Active Subunit of the Aggregatibacter actinomycetemcomitans Cytolethal Distending Toxin Is Dependent upon Cellugyrin (Synaptogyrin 2), a Host Cell Non-Neuronal Paralog of the Synaptic Vesicle Protein, Synaptogyrin 1"

    Article Title: Internalization of the Active Subunit of the Aggregatibacter actinomycetemcomitans Cytolethal Distending Toxin Is Dependent upon Cellugyrin (Synaptogyrin 2), a Host Cell Non-Neuronal Paralog of the Synaptic Vesicle Protein, Synaptogyrin 1

    Journal: Frontiers in Cellular and Infection Microbiology

    doi: 10.3389/fcimb.2017.00469

    Comparative toxic effects of Cdt on Jurkat WT and Jurkat Cg− cells. (A) Shows the effect of Cdt on cell cycle arrest; Jurkat WT and Jurkat Cg− cells were incubated for 16 h in the presence of 0–5 pg/ml Cdt. Cells were stained with propidium iodide and cell cycle analysis performed using flow cytometry. The percentage of G2 cells is shown as a mean ± SEM for three experiments each performed in triplicate; solid bars represent Jurkat WT cells and hashed bars Jurkat Cg− cells. (B) Shows the effect of Cdt on apoptosis; cells were treated with 0–25 pg/ml Cdt for 48 h analyzed for DNA strand breaks using the TUNEL assay. Results are expressed as the mean percentage of TUNEL positive cells ± SEM for three experiments (); solid bars represent Jurkat WT cells and hashed bars Jurkat Cg− cells. * Indicates statistical significance ( p
    Figure Legend Snippet: Comparative toxic effects of Cdt on Jurkat WT and Jurkat Cg− cells. (A) Shows the effect of Cdt on cell cycle arrest; Jurkat WT and Jurkat Cg− cells were incubated for 16 h in the presence of 0–5 pg/ml Cdt. Cells were stained with propidium iodide and cell cycle analysis performed using flow cytometry. The percentage of G2 cells is shown as a mean ± SEM for three experiments each performed in triplicate; solid bars represent Jurkat WT cells and hashed bars Jurkat Cg− cells. (B) Shows the effect of Cdt on apoptosis; cells were treated with 0–25 pg/ml Cdt for 48 h analyzed for DNA strand breaks using the TUNEL assay. Results are expressed as the mean percentage of TUNEL positive cells ± SEM for three experiments (); solid bars represent Jurkat WT cells and hashed bars Jurkat Cg− cells. * Indicates statistical significance ( p

    Techniques Used: Incubation, Staining, Cell Cycle Assay, Flow Cytometry, Cytometry, TUNEL Assay

    6) Product Images from "Exposure of Lymphocytes to High Doses of Actinobacillus actinomycetemcomitans Cytolethal Distending Toxin Induces Rapid Onset of Apoptosis-Mediated DNA Fragmentation "

    Article Title: Exposure of Lymphocytes to High Doses of Actinobacillus actinomycetemcomitans Cytolethal Distending Toxin Induces Rapid Onset of Apoptosis-Mediated DNA Fragmentation

    Journal: Infection and Immunity

    doi: 10.1128/IAI.74.4.2080-2092.2006

    Effect of bcl-2 overexpression on Cdt-induced G 2 arrest and DNA fragmentation. Jurkat neo and Jurkat bcl-2 cells were incubated in the presence of medium alone (Ctl), 0.05 ng/ml Cdt, or 5 ng/ml Cdt for 18 h; the cells were then stained with propidium iodide and by TUNEL to measure cell cycle distribution (panels on the right) and DNA fragmentation (panels on the left), respectively. The percentages of cells in the phases of the cell cycle are indicated, as are the percentages of cells exhibiting DNA fragmentation. The results are representative of at least three experiments.
    Figure Legend Snippet: Effect of bcl-2 overexpression on Cdt-induced G 2 arrest and DNA fragmentation. Jurkat neo and Jurkat bcl-2 cells were incubated in the presence of medium alone (Ctl), 0.05 ng/ml Cdt, or 5 ng/ml Cdt for 18 h; the cells were then stained with propidium iodide and by TUNEL to measure cell cycle distribution (panels on the right) and DNA fragmentation (panels on the left), respectively. The percentages of cells in the phases of the cell cycle are indicated, as are the percentages of cells exhibiting DNA fragmentation. The results are representative of at least three experiments.

    Techniques Used: Over Expression, Incubation, CTL Assay, Staining, TUNEL Assay

    Comparison of the effects of Cdt on cell cycle arrest, BrdU incorporation, and DNA fragmentation. Jurkat cells were treated with medium (control) or a low dose (0.05 ng/ml) or high dose (5 ng/ml) of Cdt for 18 h. The cells were then analyzed by flow cytometry to determine the cell cycle distribution using propidium iodide (A) and BrdU incorporation in order to directly assess the percentage of cells in the S phase (B) and DNA fragmentation (TUNEL) along with the cell cycle (Hoechst fluorescence) (C). The percentages of cells in the phases of the cell cycle (based on propidium iodide fluorescence) are shown in panel A. The percentages of S-phase cells determined by BrdU incorporation are indicated in panel B; cells that exhibited fluorescence greater than the fluorescence observed in controls (indicated by a line) were considered to be positive. The percentages of cells exhibiting DNA fragmentation are indicated in panel C; fluorescence values greater than the fluorescence observed in cells treated with the control antibody (indicated by a line) were considered to be positive. The results are representative of at least three experiments.
    Figure Legend Snippet: Comparison of the effects of Cdt on cell cycle arrest, BrdU incorporation, and DNA fragmentation. Jurkat cells were treated with medium (control) or a low dose (0.05 ng/ml) or high dose (5 ng/ml) of Cdt for 18 h. The cells were then analyzed by flow cytometry to determine the cell cycle distribution using propidium iodide (A) and BrdU incorporation in order to directly assess the percentage of cells in the S phase (B) and DNA fragmentation (TUNEL) along with the cell cycle (Hoechst fluorescence) (C). The percentages of cells in the phases of the cell cycle (based on propidium iodide fluorescence) are shown in panel A. The percentages of S-phase cells determined by BrdU incorporation are indicated in panel B; cells that exhibited fluorescence greater than the fluorescence observed in controls (indicated by a line) were considered to be positive. The percentages of cells exhibiting DNA fragmentation are indicated in panel C; fluorescence values greater than the fluorescence observed in cells treated with the control antibody (indicated by a line) were considered to be positive. The results are representative of at least three experiments.

    Techniques Used: BrdU Incorporation Assay, Flow Cytometry, Cytometry, TUNEL Assay, Fluorescence

    Effect of zvad on Cdt-induced G 2 arrest and DNA fragmentation. Jurkat cells were pretreated with 50 μM zvad or medium for 30 min; this was followed by addition of either medium (Ctl) or Cdt. Cells were incubated for 18 h and then analyzed for DNA fragmentation (panels on the left) and cell cycle distribution (panels on the right) as described in Materials and Methods. The percentages of cells in the phases of the cell cycle are indicated, as are the percentages of cells exhibiting DNA fragmentation; the regions where there was positive dUTP-FITC fluorescence are indicated by the bars, and the results were based upon control samples.
    Figure Legend Snippet: Effect of zvad on Cdt-induced G 2 arrest and DNA fragmentation. Jurkat cells were pretreated with 50 μM zvad or medium for 30 min; this was followed by addition of either medium (Ctl) or Cdt. Cells were incubated for 18 h and then analyzed for DNA fragmentation (panels on the left) and cell cycle distribution (panels on the right) as described in Materials and Methods. The percentages of cells in the phases of the cell cycle are indicated, as are the percentages of cells exhibiting DNA fragmentation; the regions where there was positive dUTP-FITC fluorescence are indicated by the bars, and the results were based upon control samples.

    Techniques Used: CTL Assay, Incubation, Fluorescence

    Effect of Cdt holotoxin on lymphocyte G 2 arrest and DNA fragmentation. Jurkat cells were treated with various concentrations of Cdt holotoxin for 18 h. The cells were then analyzed by flow cytometry to determine both the cell cycle distribution and the presence of DNA fragmentation as described in Methods and Materials. The percentage of G 2 cells (•) and the percentage of cells exhibiting DNA fragmentation (▪) are plotted versus Cdt concentration; the data are means ± standard deviations for three experiments. For control cells exposed to medium 9.1% of the cells were in the G 2 phase; 4.9% of the control cells exhibited DNA fragmentation.
    Figure Legend Snippet: Effect of Cdt holotoxin on lymphocyte G 2 arrest and DNA fragmentation. Jurkat cells were treated with various concentrations of Cdt holotoxin for 18 h. The cells were then analyzed by flow cytometry to determine both the cell cycle distribution and the presence of DNA fragmentation as described in Methods and Materials. The percentage of G 2 cells (•) and the percentage of cells exhibiting DNA fragmentation (▪) are plotted versus Cdt concentration; the data are means ± standard deviations for three experiments. For control cells exposed to medium 9.1% of the cells were in the G 2 phase; 4.9% of the control cells exhibited DNA fragmentation.

    Techniques Used: Flow Cytometry, Cytometry, Concentration Assay

    Kinetics of Cdt-induced DNA degradation. Jurkat cells were treated with Cdt or medium for 24, 48, or 72 h. The cells were then assessed for DNA fragmentation using the TUNEL assay and FACS. The bars indicate regions where there was positive fluorescence; the percentages of cells in these regions that exhibited DNA fragmentation are indicated, and the degrees of fragmentation are indicated by the MCF values. The results are representative of three experiments.
    Figure Legend Snippet: Kinetics of Cdt-induced DNA degradation. Jurkat cells were treated with Cdt or medium for 24, 48, or 72 h. The cells were then assessed for DNA fragmentation using the TUNEL assay and FACS. The bars indicate regions where there was positive fluorescence; the percentages of cells in these regions that exhibited DNA fragmentation are indicated, and the degrees of fragmentation are indicated by the MCF values. The results are representative of three experiments.

    Techniques Used: TUNEL Assay, FACS, Fluorescence

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    Article Snippet: Intensities of the fluorescent products in cells were measured with a LS55 luminescence spectrometer (Perkin Elmer, Norwalk, CT, USA). .. Quantification of DNA fragmentation DNA fragmentation was quantified using a cellular DNA fragmentation enzyme-linked immunosorbent assay (ELISA) kit (Boehringer Mannheim, Indianapolis, IN, USA) . .. Briefly, MC3T3-E1 cells (2 x 105 ) were subcultured in 24-well tissue culture plates and labeled with 5-bromo-2'-deoxyuridine (BrdU) at 100 µM overnight.

    Article Title: Major Contribution of Caspase-9 to Honokiol-Induced Apoptotic Insults to Human Drug-Resistant Glioblastoma Cells
    Article Snippet: Immunoreactive proteins were detected using an enhanced chemiluminescence reagent (PerkinElmer, Waltham MA, USA) and then imaged using a digital analyzer (Syngene, Cambridge, UK) and a densitometry software (Syngene). .. Quantification of DNA Fragmentation DNA fragmentation in human U87-MG glioblastoma cells was quantified using a cellular DNA fragmentation enzyme-linked immunosorbent assay (ELISA) kit (Boehringer Mannheim, Indianapolis, IN, USA), as described previously [ ]. .. Briefly, 2 × 105 human U87-MG-R9 glioblastoma cells were subcultured in 24-well tissue culture plates and labeled with 5-bromo-2′-deoxyuridine (BrdU) overnight.

    Article Title: Nanoparticles prepared from the water extract of Gusuibu (Drynaria fortunei J. Sm.) protects osteoblasts against insults and promotes cell maturation
    Article Snippet: .. Quantification of DNA fragmentation DNA fragmentation in rat osteoblasts was quantified by the cellular DNA fragmentation enzyme-linked immunosorbent assay kit (Boehringer Mannheim, Indianapolis, IN) as described previously. .. Briefly, rat osteoblasts (2 × 105 cells) were subcultured in 24-well tissue culture plates and labeled with BrdU overnight.

    Article Title: Improved effects of honokiol on temozolomide-induced autophagy and apoptosis of drug-sensitive and -tolerant glioma cells
    Article Snippet: The peptide was conjugated to 7-amino-4-trifluoromethyl coumarin for fluorescence detection. .. Quantification of DNA fragmentation DNA fragmentation in human U87-MG glioma cells was quantified using a cellular DNA fragmentation enzyme-linked immunosorbent assay (ELISA) kit (Boehringer Mannheim, Indianapolis, IN, USA) as described previously [ ]. .. Briefly, 2 × 105 human U87-MG glioma cells were subcultured in 24-well tissue culture plates and labeled with 5-bromo-2’-deoxyuridine (BrdU) overnight.

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    Article Title: Chloroquine enhances TRAIL-mediated apoptosis through up-regulation of DR5 by stabilization of mRNA and protein in cancer cells
    Article Snippet: After the fixation, the cells were washed with PBS and a 300 nM 4′,6′-diamidino-2-phenylindole solution (Roche, Mannheim, Germany) was added to the fixed cells for 5 min. After the nuclei were stained, the cells were examined by fluorescence microscopy. .. DNA Fragmentation Assay DNA fragmentation was performed using the Cell Death Detection ELISAPLUS kit (Boehringer Mannheim; Indianapolis, USA). .. Briefly, cells were centrifuged for 10 min at 200 × g, the supernatant was removed, and pellet was lysed for 30 min. After centrifuging the plate again at 200 × g for 10 min, and the supernatant that contained the cytoplasmic histone-associated DNA fragments was collected and incubated with an immobilized anti-histone antibody.

    other:

    Article Title: Prophylactic Administration of Nanocurcumin Abates the Incidence of Liver Toxicity Induced by an Overdose of Copper Sulfate: Role of CYP4502E1, NF-κB and Bax Expressions
    Article Snippet: DNA Fragmentation DNA fragmentation quantitated by measuring oligonucleosome-bound DNA using an ELlSA kit (Boehringer, Mannheim, Germany).

    In Situ:

    Article Title: Role of Integrin-Linked Kinase in Nerve Growth Factor-Stimulated Neurite Outgrowth
    Article Snippet: For terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling (TUNEL) labeling, PC12 cells were plated onto coverslips coated with poly- d -lysine (100 μg/ml) and collagen I. .. After a 48 hr drug treatment, DNA fragmentation was measured in PC12 cells using the In Situ Cell Death Detection Kit (Fluorescein; Boehringer Mannheim Biochemicals, Indianapolis, IN) according to the methods of . .. Cells were then washed in PBS and mounted in Vectashield mounting media containing DAPI (Vector Laboratories, Burlingame, CA).

    Article Title: Bax Deletion Further Orders the Cell Death Pathway in Cerebellar Granule Cells and Suggests a Caspase-independent Pathway to Cell Death
    Article Snippet: TUNEL Staining At 7 d in vitro, culture medium from cerebellar granule cells was replaced with either K5+S, K5+S plus 100 μM BAF, or fresh K25+S after washing the cells once with the appropriate medium. .. At 24 h after this treatment, cells were examined for DNA fragmentation using the In Situ Cell Death Detection Kit (Fluorescein; Boehringer Mannheim Biochemicals , Indianapolis, IN) according to the manufacturer's instructions. ..

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    Boehringer Mannheim klenow fragment
    The effect of Ku on the 3′→5′ exonuclease activity of WRN, <t>Klenow</t> and exo <t>III</t> on a substrate containing 8-oxoA. DNA substrate containing 8-oxoA was incubated without enzyme (–enzyme) or with WRN (180 fmol), Klenow (2 U) or exo III (1 U) in the absence or presence of Ku (64 fmol) at 37°C for 1 h. The labeled reaction products were analyzed and visualized as described earlier.
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    Apoptosis in rat peripheral blood lymphocytes exposed to CI-994 for 4 or 24 hours. Mitogen (concanavalin A, 0.63 μg/ml) was added to all lymphocyte cultures at time zero. <t>Apoptotic</t> cells characterized by condensed <t>DNA</t> were visualized by fluorescent microscopy and acridine orange/ethidium bromide stain. Data represent the mean ± SE of at least 4 individual experiments. * Significantly different from control using Kruskal-Wallis one way ANOVA on ranked data and Dunn's post hoc test ( p
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    Confirmation of gene disruption by Southern analysis. Lane 1, <t>DNA</t> from the control strain; lane 2, DNA from the disrupted strain; lane 3, Dig-labelled DNA molecular weight marker III (Boehringer <t>Mannheim).</t>
    Random Primed Dna Dig Labelling Kit, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Southern hybridization of Apa I (a)-, Bam HI (b)-, and Hin dIII (c)-digested DNAs of Borrelia isolates with <t>digoxigenin-labeled</t> Co53 DNA as a probe. Lanes: 1, CA434; 2, CA435; 3, Co53; 4, B. parkeri ; 5, CA4. Southern hybridization and detection of DNA which hybridized to the probe were done as recommended by Boehringer Mannheim for the digoxigenin DNA labeling and detection kit.
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    The effect of Ku on the 3′→5′ exonuclease activity of WRN, Klenow and exo III on a substrate containing 8-oxoA. DNA substrate containing 8-oxoA was incubated without enzyme (–enzyme) or with WRN (180 fmol), Klenow (2 U) or exo III (1 U) in the absence or presence of Ku (64 fmol) at 37°C for 1 h. The labeled reaction products were analyzed and visualized as described earlier.

    Journal: Nucleic Acids Research

    Article Title: A functional interaction of Ku with Werner exonuclease facilitates digestion of damaged DNA

    doi:

    Figure Lengend Snippet: The effect of Ku on the 3′→5′ exonuclease activity of WRN, Klenow and exo III on a substrate containing 8-oxoA. DNA substrate containing 8-oxoA was incubated without enzyme (–enzyme) or with WRN (180 fmol), Klenow (2 U) or exo III (1 U) in the absence or presence of Ku (64 fmol) at 37°C for 1 h. The labeled reaction products were analyzed and visualized as described earlier.

    Article Snippet: Exonuclease III (exo III) and the Klenow fragment of Escherichia coli were purchased from Boehringer Mannheim and T4 polynucleotide kinase was from New England Biolabs (Beverly, MA).

    Techniques: Activity Assay, Incubation, Labeling

    Apoptosis in rat peripheral blood lymphocytes exposed to CI-994 for 4 or 24 hours. Mitogen (concanavalin A, 0.63 μg/ml) was added to all lymphocyte cultures at time zero. Apoptotic cells characterized by condensed DNA were visualized by fluorescent microscopy and acridine orange/ethidium bromide stain. Data represent the mean ± SE of at least 4 individual experiments. * Significantly different from control using Kruskal-Wallis one way ANOVA on ranked data and Dunn's post hoc test ( p

    Journal: Journal of Biomedicine and Biotechnology

    Article Title: Induction of apoptosis in rat peripheral blood lymphocytes by the anticancer drug CI-994 (acetyldinaline)

    doi: 10.1155/S1110724301000146

    Figure Lengend Snippet: Apoptosis in rat peripheral blood lymphocytes exposed to CI-994 for 4 or 24 hours. Mitogen (concanavalin A, 0.63 μg/ml) was added to all lymphocyte cultures at time zero. Apoptotic cells characterized by condensed DNA were visualized by fluorescent microscopy and acridine orange/ethidium bromide stain. Data represent the mean ± SE of at least 4 individual experiments. * Significantly different from control using Kruskal-Wallis one way ANOVA on ranked data and Dunn's post hoc test ( p

    Article Snippet: DNA fragmentation assay DNA was isolated from lymphocytes using the apoptotic DNA-Ladder kit (Boehringer Mannheim, Indianapolis, IN).

    Techniques: Microscopy, Staining

    Confirmation of gene disruption by Southern analysis. Lane 1, DNA from the control strain; lane 2, DNA from the disrupted strain; lane 3, Dig-labelled DNA molecular weight marker III (Boehringer Mannheim).

    Journal: Applied and Environmental Microbiology

    Article Title: Isolation, Nucleotide Sequence, and Physiological Relevance of the Gene Encoding Triose Phosphate Isomerase from Kluyveromyces lactis

    doi:

    Figure Lengend Snippet: Confirmation of gene disruption by Southern analysis. Lane 1, DNA from the control strain; lane 2, DNA from the disrupted strain; lane 3, Dig-labelled DNA molecular weight marker III (Boehringer Mannheim).

    Article Snippet: Hybridization was performed by using as a probe the Bgl II- Csp 45 I fragment, Dig-labelled (Random Primed DNA Dig-labelling kit; Boehringer Mannheim) at 48°C in 50% formamide, 5× SSC, 0.2% SDS, and 2.5% blocking solution.

    Techniques: Molecular Weight, Marker

    Southern hybridization of Apa I (a)-, Bam HI (b)-, and Hin dIII (c)-digested DNAs of Borrelia isolates with digoxigenin-labeled Co53 DNA as a probe. Lanes: 1, CA434; 2, CA435; 3, Co53; 4, B. parkeri ; 5, CA4. Southern hybridization and detection of DNA which hybridized to the probe were done as recommended by Boehringer Mannheim for the digoxigenin DNA labeling and detection kit.

    Journal: Journal of Clinical Microbiology

    Article Title: Genetic Characteristics of Borrelia coriaceae Isolates from the Soft Tick Ornithodoros coriaceus (Acari: Argasidae)

    doi:

    Figure Lengend Snippet: Southern hybridization of Apa I (a)-, Bam HI (b)-, and Hin dIII (c)-digested DNAs of Borrelia isolates with digoxigenin-labeled Co53 DNA as a probe. Lanes: 1, CA434; 2, CA435; 3, Co53; 4, B. parkeri ; 5, CA4. Southern hybridization and detection of DNA which hybridized to the probe were done as recommended by Boehringer Mannheim for the digoxigenin DNA labeling and detection kit.

    Article Snippet: The probe was prepared by restricting total DNA of strain Co53 with Hin dIII and labeling the fragments with digoxigenin using the Genius DNA labeling kit as described by the manufacturer (Boehringer Mannheim).

    Techniques: Hybridization, Labeling, DNA Labeling