dmtu  (Millipore)

 
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    Name:
    N N Dimethylthiourea
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    Catalog Number:
    D188700
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    Structured Review

    Millipore dmtu
    N N Dimethylthiourea

    https://www.bioz.com/result/dmtu/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dmtu - by Bioz Stars, 2021-04
    93/100 stars

    Images

    1) Product Images from "Perturbation of Polyamine Catabolism Can Strongly Affect Root Development and Xylem Differentiation 1Perturbation of Polyamine Catabolism Can Strongly Affect Root Development and Xylem Differentiation 1 [W]"

    Article Title: Perturbation of Polyamine Catabolism Can Strongly Affect Root Development and Xylem Differentiation 1Perturbation of Polyamine Catabolism Can Strongly Affect Root Development and Xylem Differentiation 1 [W]

    Journal: Plant Physiology

    doi: 10.1104/pp.111.173153

    Effect of Spd, DMTU, G3, and ABAL exogenous supply on cell cycle phase distributions in the maize primary root tip. A, DNA fluorescence pulse area histograms of DAPI-stained nuclei isolated from maize root tips from 5-d-old plants, grown for 24 h in aerated
    Figure Legend Snippet: Effect of Spd, DMTU, G3, and ABAL exogenous supply on cell cycle phase distributions in the maize primary root tip. A, DNA fluorescence pulse area histograms of DAPI-stained nuclei isolated from maize root tips from 5-d-old plants, grown for 24 h in aerated

    Techniques Used: Fluorescence, Staining, Isolation

    Effect of exogenously supplied Spd, DMTU, G3, and ABAL on DNA fragmentation detected by TUNEL in situ assay in cross sections of the maize primary root apex. A, C, E, G, and I, TUNEL staining of 10-μm-thick transversal sections at 1,000 μm
    Figure Legend Snippet: Effect of exogenously supplied Spd, DMTU, G3, and ABAL on DNA fragmentation detected by TUNEL in situ assay in cross sections of the maize primary root apex. A, C, E, G, and I, TUNEL staining of 10-μm-thick transversal sections at 1,000 μm

    Techniques Used: TUNEL Assay, In Situ, Staining

    2) Product Images from "Red Ginseng Extract Attenuates Kainate-Induced Excitotoxicity by Antioxidative Effects"

    Article Title: Red Ginseng Extract Attenuates Kainate-Induced Excitotoxicity by Antioxidative Effects

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    doi: 10.1155/2012/479016

    Effect of RGE and scavengers on MDA levels in KA-treated hippocampal tissue homogenates. Male mice were grouped ( n = 5 or 6/group) and pretreated (i.p. injection) with RGE (30–200 mg/kg) and scavengers such as trolox (50 mg/kg, i.p.) and DMTU (50 mg/kg, i.p.), or NaCl (0.9%). Thirty minutes after the final RGE or saline pretreatment, seizures in the KA, scavengers + KA, and RGE + KA groups were induced by KA injection (30 mg/kg, i.p.); the mice in the saline group received an equal volume of 0.9% NaCl. All data are presented as means ± SE. ### P
    Figure Legend Snippet: Effect of RGE and scavengers on MDA levels in KA-treated hippocampal tissue homogenates. Male mice were grouped ( n = 5 or 6/group) and pretreated (i.p. injection) with RGE (30–200 mg/kg) and scavengers such as trolox (50 mg/kg, i.p.) and DMTU (50 mg/kg, i.p.), or NaCl (0.9%). Thirty minutes after the final RGE or saline pretreatment, seizures in the KA, scavengers + KA, and RGE + KA groups were induced by KA injection (30 mg/kg, i.p.); the mice in the saline group received an equal volume of 0.9% NaCl. All data are presented as means ± SE. ### P

    Techniques Used: Multiple Displacement Amplification, Mouse Assay, Injection

    Effects of RGE and scavengers against oxidative stress in primary cultured hippocampal cells. (a) Concentration data for KA-induced ROS levels in primary cultured hippocampal neurons. Examination of the dose effect of KA on neuronal ROS level by the DCFH-DA assay. Neurons were exposed to KA at concentrations of 0, 30, 50, 70, and 100 μ M for 48 h. (b) Protection of hippocampal neurons against KA-induced ROS levels by RGE. Neurons were exposed to 100 μ M KA at 1 h after 0.01–1 mg/mL of RGE, trolox (100 μ M), or DMTU (100 μ M) treatment. All data are presented as means ± SE. ### P
    Figure Legend Snippet: Effects of RGE and scavengers against oxidative stress in primary cultured hippocampal cells. (a) Concentration data for KA-induced ROS levels in primary cultured hippocampal neurons. Examination of the dose effect of KA on neuronal ROS level by the DCFH-DA assay. Neurons were exposed to KA at concentrations of 0, 30, 50, 70, and 100 μ M for 48 h. (b) Protection of hippocampal neurons against KA-induced ROS levels by RGE. Neurons were exposed to 100 μ M KA at 1 h after 0.01–1 mg/mL of RGE, trolox (100 μ M), or DMTU (100 μ M) treatment. All data are presented as means ± SE. ### P

    Techniques Used: Cell Culture, Concentration Assay, DCFH-DA Assay

    Effect of RGE and scavengers on BDNF levels in KA-treated hippocampal neurons. Immunoblots of lysed embryonic rat hippocampi 2 days following administration of RGE or KA are shown. Neurons were exposed to KA at concentrations of 0, 30, 50, 70, and 100 μ M for 48 h. Neurons were exposed to 100 μ M KA at 1 h after 0.01–1.0 mg/mL of RGE, trolox (100 μ M), and DMTU (100 μ M) treatment. GAPDH levels were measured to confirm equal protein loading.
    Figure Legend Snippet: Effect of RGE and scavengers on BDNF levels in KA-treated hippocampal neurons. Immunoblots of lysed embryonic rat hippocampi 2 days following administration of RGE or KA are shown. Neurons were exposed to KA at concentrations of 0, 30, 50, 70, and 100 μ M for 48 h. Neurons were exposed to 100 μ M KA at 1 h after 0.01–1.0 mg/mL of RGE, trolox (100 μ M), and DMTU (100 μ M) treatment. GAPDH levels were measured to confirm equal protein loading.

    Techniques Used: Western Blot

    Effect of RGE and scavengers on [Ca 2+ ] i influx in primary cultured hippocampal cells. (a) Concentration data for KA-induced [Ca 2+ ] i in primary cultured hippocampal neurons. Examination of the dose effect of KA on neuronal [Ca 2+ ] i by the Ca 2+ indicator with fura-4. Neurons were exposed to KA at concentrations of 0, 30, 50, 70, and 100 μ M for 48 h. (b) Protection of hippocampal neurons against KA-induced [Ca 2+ ] i by RGE. Neurons were exposed to 100 μ M KA at 1 h after 0.01–1.0 mg/mL of RGE, trolox (100 μ M), or DMTU (100 μ M) treatment. All data are presented as means ± SE. ### P
    Figure Legend Snippet: Effect of RGE and scavengers on [Ca 2+ ] i influx in primary cultured hippocampal cells. (a) Concentration data for KA-induced [Ca 2+ ] i in primary cultured hippocampal neurons. Examination of the dose effect of KA on neuronal [Ca 2+ ] i by the Ca 2+ indicator with fura-4. Neurons were exposed to KA at concentrations of 0, 30, 50, 70, and 100 μ M for 48 h. (b) Protection of hippocampal neurons against KA-induced [Ca 2+ ] i by RGE. Neurons were exposed to 100 μ M KA at 1 h after 0.01–1.0 mg/mL of RGE, trolox (100 μ M), or DMTU (100 μ M) treatment. All data are presented as means ± SE. ### P

    Techniques Used: Cell Culture, Concentration Assay

    RGE prevents KA-induced neuronal loss in primary cultured hippocampal cells. (a) Concentration data for KA-induced toxicity in primary cultured hippocampal neurons. Examination of the dose effect of KA on neuronal viability by the MTT assay. Neurons were exposed to KA at concentrations of 0, 30, 50, 70, and 100 μ M for 48 h. (b) Protection of hippocampal neurons against KA-induced cell loss by RGE. Neurons were exposed to 100 μ M KA at 1 h after 0.01–1.0 mg/mL of RGE, Trolox (100 μ M), or DMTU (100 μ M) treatment. Cell viability at 48 h after KA exposure was measured by the MTT assay. All data are presented as means ± SE. ### P
    Figure Legend Snippet: RGE prevents KA-induced neuronal loss in primary cultured hippocampal cells. (a) Concentration data for KA-induced toxicity in primary cultured hippocampal neurons. Examination of the dose effect of KA on neuronal viability by the MTT assay. Neurons were exposed to KA at concentrations of 0, 30, 50, 70, and 100 μ M for 48 h. (b) Protection of hippocampal neurons against KA-induced cell loss by RGE. Neurons were exposed to 100 μ M KA at 1 h after 0.01–1.0 mg/mL of RGE, Trolox (100 μ M), or DMTU (100 μ M) treatment. Cell viability at 48 h after KA exposure was measured by the MTT assay. All data are presented as means ± SE. ### P

    Techniques Used: Cell Culture, Concentration Assay, MTT Assay

    Reduction activity of RGE on • OH. (a) ESR spectra of the DMPO/ • OH adduct were generated in a Fenton Reaction System. The solutions with a final volume of 0.1 mL contained 2.0 mM ferrous sulfate, 2.0 mM H 2 O 2 , and 100 mM phosphate buffer (pH 7.2). Reactions were started by the addition of ferrous ammonium sulfate (2.0 mM final concentration), and the steady-state ESR spectra were recorded at 15 min after the Fenton Reaction. Upper line, DMPO/ • OH adduct; lower line, RGE (1.0 mg/mL). (b) Time course of • OH degradation induced by the Fenton Reaction System. DMPO/ • OH adduct, RGE (1.0 mg/mL), trolox (1.0 mM), and DMTU (1 mM).
    Figure Legend Snippet: Reduction activity of RGE on • OH. (a) ESR spectra of the DMPO/ • OH adduct were generated in a Fenton Reaction System. The solutions with a final volume of 0.1 mL contained 2.0 mM ferrous sulfate, 2.0 mM H 2 O 2 , and 100 mM phosphate buffer (pH 7.2). Reactions were started by the addition of ferrous ammonium sulfate (2.0 mM final concentration), and the steady-state ESR spectra were recorded at 15 min after the Fenton Reaction. Upper line, DMPO/ • OH adduct; lower line, RGE (1.0 mg/mL). (b) Time course of • OH degradation induced by the Fenton Reaction System. DMPO/ • OH adduct, RGE (1.0 mg/mL), trolox (1.0 mM), and DMTU (1 mM).

    Techniques Used: Activity Assay, Electron Paramagnetic Resonance, Generated, Concentration Assay

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    Article Snippet: DPI chloride, Evans blue, 4,5-dihydroxy-1,3-benzene-disulfonic acid (Tiron), N ,N ′ -dimethylthiourea (DMTU), were from Sigma (St. Louis, MO, USA).

    Article Title: UMOylation Occurs in Acute Kidney Injury and Plays a Cytoprotective Role
    Article Snippet: Cisplatin, dimethyl sulfoxide (DMSO), dimethylthiourea (DMTU), nacetylcysteine (NAC), sodium azide, pifithrin-α and N-Ethylmaleimide (NEM) were purchased from Sigma-Aldrich (St. Louis, MO).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Mitochondrial Superoxide Mediates Doxorubicin-Induced Keratinocyte Apoptosis through Oxidative Modification of ERK and Bcl-2 Ubiquitination
    Article Snippet: Doxorubicin, Mn(III)tetrakis(4-benzoic acid) porphyrin chloride (MnTBAP) and concanamycin A were obtained from EMD Biosciences (La Jolla, CA). .. N -acetyl cysteine (NAC), cell permeable catalase (catalase-polyethylene glycol; CAT), dimethylthiourea (DMTU), lactacystin, and antibody for ubiquitin were obtained from Sigma Chemical (St. Louis, MO). .. Hoechst 33342, di-hydrodichlorofluorescein diacetate (H2 DCF-DA), dihydroethidium (DHE), and Mitosox™ were obtained from Molecular Probes (Eugene, OR).

    Modification:

    Article Title: Glucose-dependent trans-plasma membrane electron transport and p70S6k phosphorylation in skeletal muscle cells
    Article Snippet: 2.1 Materials C2C12 myoblasts, a mouse muscle cell line, and L6 myoblasts, a rat muscle cell line, were obtained from American Type Culture Collection (Manassas, VA, USA). .. Dulbecco's modified Eagle's medium-low glucose (DMEM), phosphate buffered saline (PBS), penicillin-streptomycin, trypsin-EDTA, phenazine methosulfate (PMS), D -glucose, pyruvate, superoxide dismutase (SOD), 2-deoxy-D -glucose (2DG), dehydroepiandrosterone (DHEA), (2E)-3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one (3PO), 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid tetrakis (acetoxymethyl ester) (BAPTA-AM), diphenyleneiodonium (DPI), N,N′-dimethylthiourea (DMTU), 4-hydroxy-TEMPO (Tempol), and glucose oxidase were purchased from Sigma Aldrich (St. Louis, MO, USA). .. FetalPlex animal serum complex was purchased from Gemini Bio-Products (Woodland, CA, USA).

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  • 93
    Millipore dmtu
    Mitochondrial membrane graphs of MDA-MB-231 cells exposed to ESE-one in the presence or absence of several scavengers of reactive oxygen species (tiron, <t>trolox</t> and <t>DMTU).</t> ESE-one only exposure resulted in depolarization of the mitochondrial membrane potential in MDA-MB-231 cells and tiron exposure resulted in an insignificant decrease in membrane depolarization. A represents polarized cells and B represents depolarized cells. Trolox and DMTU did not have an opposing effect on the membrane depolarization exerted by ESE-one (A indicates polarized population and B indicates depolarized population). ( a ) Cells cultured in complete growth medium, ( b ) vehicle-treated cells, ( c ) cells exposed to ESE-one, ( d ) cells exposed to carbonyl cyanide 3-chlorophenylhydrazone (CCCP), ( e ) cells exposed to tiron only, ( f ) cells coexposed to tiron and ESE-one, ( g ) cells exposed to trolox only, ( h ) cells coexposed to trolox and ESE-one, ( i ) cells exposed to DMTU only, ( j ) cells coexposed to DMTU and ESE-one.
    Dmtu, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dmtu/product/Millipore
    Average 93 stars, based on 1 article reviews
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    99
    Millipore dimethyl thiourea dmtu
    ROS scavenging capacity of H. inuloides metabolites and semisynthetic derivatives. <t>C=NDGA</t> (superoxide); pyruvate (H 2 O 2 ); <t>DMTU</t> (OH − ); GSH ( 1 O 2 ); ascorbic Ac. acid (HOCl). The results were analyzed by ANOVA. The Dunnett's Multiple Comparison test was used to compare outcomes between experimental and control group. * P
    Dimethyl Thiourea Dmtu, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dimethyl thiourea dmtu/product/Millipore
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    97
    Millipore cisplatin dmtu group
    Representative micrographs of renal histopathology in mice at 72 h after <t>cisplatin</t> administration. Tubular necrosis, dilatation, and hyaline cast were observed in the cisplatin group, and the <t>DMTU</t> treatment suppressed the severity of the tissue damage. Bar = 50 μm
    Cisplatin Dmtu Group, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Mitochondrial membrane graphs of MDA-MB-231 cells exposed to ESE-one in the presence or absence of several scavengers of reactive oxygen species (tiron, trolox and DMTU). ESE-one only exposure resulted in depolarization of the mitochondrial membrane potential in MDA-MB-231 cells and tiron exposure resulted in an insignificant decrease in membrane depolarization. A represents polarized cells and B represents depolarized cells. Trolox and DMTU did not have an opposing effect on the membrane depolarization exerted by ESE-one (A indicates polarized population and B indicates depolarized population). ( a ) Cells cultured in complete growth medium, ( b ) vehicle-treated cells, ( c ) cells exposed to ESE-one, ( d ) cells exposed to carbonyl cyanide 3-chlorophenylhydrazone (CCCP), ( e ) cells exposed to tiron only, ( f ) cells coexposed to tiron and ESE-one, ( g ) cells exposed to trolox only, ( h ) cells coexposed to trolox and ESE-one, ( i ) cells exposed to DMTU only, ( j ) cells coexposed to DMTU and ESE-one.

    Journal: Molecules

    Article Title: Dysregulation of Catalase by a Sulphamoylated Estradiol Analogue Culminates in Antimitotic Activity and Cell Death Induction in Breast Cancer Cell Lines

    doi: 10.3390/molecules26030622

    Figure Lengend Snippet: Mitochondrial membrane graphs of MDA-MB-231 cells exposed to ESE-one in the presence or absence of several scavengers of reactive oxygen species (tiron, trolox and DMTU). ESE-one only exposure resulted in depolarization of the mitochondrial membrane potential in MDA-MB-231 cells and tiron exposure resulted in an insignificant decrease in membrane depolarization. A represents polarized cells and B represents depolarized cells. Trolox and DMTU did not have an opposing effect on the membrane depolarization exerted by ESE-one (A indicates polarized population and B indicates depolarized population). ( a ) Cells cultured in complete growth medium, ( b ) vehicle-treated cells, ( c ) cells exposed to ESE-one, ( d ) cells exposed to carbonyl cyanide 3-chlorophenylhydrazone (CCCP), ( e ) cells exposed to tiron only, ( f ) cells coexposed to tiron and ESE-one, ( g ) cells exposed to trolox only, ( h ) cells coexposed to trolox and ESE-one, ( i ) cells exposed to DMTU only, ( j ) cells coexposed to DMTU and ESE-one.

    Article Snippet: Propidium iodide, trolox, DMTU and tiron were obtained from Sigma Chemical Co. (Sigma Chemical Co., St. Louis, MO, USA).

    Techniques: Multiple Displacement Amplification, Cell Culture

    Mitochondrial membrane graphs of MCF-7 cells treated with ESE-one in the presence or absence of several scavengers of reactive oxygen species (tiron, trolox and DMTU). ESE-one only exposure resulted in depolarization of the mitochondrial membrane potential in MCF-7 cells and tiron countered that effect significantly. A represents polarized cells and B represents depolarized cells. Trolox and DMTU did not have an opposing effect on the membrane depolarization exerted by ESE-one (A indicates polarized population and B indicates depolarized population). ( a ) Cells cultured in complete growth medium, ( b ) vehicle-treated cells, ( c ) cells exposed to ESE-one, ( d ) cells exposed to carbonyl cyanide 3-chlorophenylhydrazone (CCCP), ( e ) cells exposed to tiron only, ( f ) cells coexposed to tiron and ESE-one, ( g ) cells exposed to trolox only, ( h ) cells coexposed to trolox and ESE-one, ( i ) cells exposed to DMTU only, ( j ) cells coexposed to DMTU and ESE-one.

    Journal: Molecules

    Article Title: Dysregulation of Catalase by a Sulphamoylated Estradiol Analogue Culminates in Antimitotic Activity and Cell Death Induction in Breast Cancer Cell Lines

    doi: 10.3390/molecules26030622

    Figure Lengend Snippet: Mitochondrial membrane graphs of MCF-7 cells treated with ESE-one in the presence or absence of several scavengers of reactive oxygen species (tiron, trolox and DMTU). ESE-one only exposure resulted in depolarization of the mitochondrial membrane potential in MCF-7 cells and tiron countered that effect significantly. A represents polarized cells and B represents depolarized cells. Trolox and DMTU did not have an opposing effect on the membrane depolarization exerted by ESE-one (A indicates polarized population and B indicates depolarized population). ( a ) Cells cultured in complete growth medium, ( b ) vehicle-treated cells, ( c ) cells exposed to ESE-one, ( d ) cells exposed to carbonyl cyanide 3-chlorophenylhydrazone (CCCP), ( e ) cells exposed to tiron only, ( f ) cells coexposed to tiron and ESE-one, ( g ) cells exposed to trolox only, ( h ) cells coexposed to trolox and ESE-one, ( i ) cells exposed to DMTU only, ( j ) cells coexposed to DMTU and ESE-one.

    Article Snippet: Propidium iodide, trolox, DMTU and tiron were obtained from Sigma Chemical Co. (Sigma Chemical Co., St. Louis, MO, USA).

    Techniques: Cell Culture

    Cell cycle progression graphs of MCF-7 and MDA-MB-231 cells exposed to ESE-one in the presence or absence of several scavengers of reactive oxygen species (tiron, trolox and DMTU) for 24 h. ESE-one exposure resulted in a G 2 /M block in both MCF-7 and MDA-MB-231 cells. Tiron and DMTU exposure significantly decreased the number of cells blocked in sub-G 1 phase in MDA-MB-231 cells and tiron as well as trolox exposure significantly decreased the number of cells blocked in G 2 /M phase in both MCF-7 and MDA-MB-231 cells. ( a ) MCF-7 cells cultured in complete growth medium, ( b ) vehicle-treated MCF-7 cells, ( c ) MCF-7 cells exposed to ESE-one only, ( d ) MDA-MB-231 cells cultured in complete growth medium, ( e ) vehicle-treated MDA-MB-231 cells, ( f ) MDA-MB-231 cells exposed to ESE-one, ( g ) MCF-7 cells exposed to tiron only, ( h ) MCF-7 cells exposed to trolox only, ( i ) MCF-7 cells exposed to DMTU only, ( j ) MCF-7 cells coexposed to tiron and ESE-one, ( k ) MCF-7 cells coexposed to trolox and ESE-one, ( l ) MCF-7 cells coexposed to DMTU and ESE-one, ( m ) MDA-MB-231 cells coexposed to tiron only, ( n ) MDA-MB-231 cells exposed to trolox only, ( o ) MDA-MB-231 cells exposed to DMTU only, ( p ) MDA-MB-231 cells coexposed to tiron and ESE-one, ( q ) MDA-MB-231 cells coexposed to trolox and ESE-one, ( r ) MDA-MB-231 cells coexposed to DMTU and ESE-one.

    Journal: Molecules

    Article Title: Dysregulation of Catalase by a Sulphamoylated Estradiol Analogue Culminates in Antimitotic Activity and Cell Death Induction in Breast Cancer Cell Lines

    doi: 10.3390/molecules26030622

    Figure Lengend Snippet: Cell cycle progression graphs of MCF-7 and MDA-MB-231 cells exposed to ESE-one in the presence or absence of several scavengers of reactive oxygen species (tiron, trolox and DMTU) for 24 h. ESE-one exposure resulted in a G 2 /M block in both MCF-7 and MDA-MB-231 cells. Tiron and DMTU exposure significantly decreased the number of cells blocked in sub-G 1 phase in MDA-MB-231 cells and tiron as well as trolox exposure significantly decreased the number of cells blocked in G 2 /M phase in both MCF-7 and MDA-MB-231 cells. ( a ) MCF-7 cells cultured in complete growth medium, ( b ) vehicle-treated MCF-7 cells, ( c ) MCF-7 cells exposed to ESE-one only, ( d ) MDA-MB-231 cells cultured in complete growth medium, ( e ) vehicle-treated MDA-MB-231 cells, ( f ) MDA-MB-231 cells exposed to ESE-one, ( g ) MCF-7 cells exposed to tiron only, ( h ) MCF-7 cells exposed to trolox only, ( i ) MCF-7 cells exposed to DMTU only, ( j ) MCF-7 cells coexposed to tiron and ESE-one, ( k ) MCF-7 cells coexposed to trolox and ESE-one, ( l ) MCF-7 cells coexposed to DMTU and ESE-one, ( m ) MDA-MB-231 cells coexposed to tiron only, ( n ) MDA-MB-231 cells exposed to trolox only, ( o ) MDA-MB-231 cells exposed to DMTU only, ( p ) MDA-MB-231 cells coexposed to tiron and ESE-one, ( q ) MDA-MB-231 cells coexposed to trolox and ESE-one, ( r ) MDA-MB-231 cells coexposed to DMTU and ESE-one.

    Article Snippet: Propidium iodide, trolox, DMTU and tiron were obtained from Sigma Chemical Co. (Sigma Chemical Co., St. Louis, MO, USA).

    Techniques: Multiple Displacement Amplification, Blocking Assay, Cell Culture

    Cell cycle progression graphs of MCF-7 and MDA-MB-231 cells exposed to ESE-one in the presence or absence of various scavengers of reactive oxygen species (tiron, trolox and DMTU) for 48 h. ESE-one exposure resulted in a significant increase in the percentage of cells occupying the sub-G 1 phase in both MCF-7 and MDA-MB-231 cells. Tiron, trolox and DMTU exposure significantly decreased the number of cells occupying the sub-G 1 phase in MCF-7 cells and tiron as well as DMTU exposure significantly decreased the number of cells present in the sub-G 1 phase in MDA-MB-231 cells. Tiron, trolox and DMTU significantly decreased the number of cells in the G 2 /M phase in MDA-MB-231 cells and only tiron had a significant effect in MCF-7 cells. ( a ) MCF-7 cells cultured in complete growth medium, ( b ) vehicle-treated MCF-7 cells, ( c ) MCF-7 cells exposed to ESE-one only, ( d ) MDA-MB-231 cells cultured in complete growth medium, ( e ) vehicle-treated MDA-MB-231 cells, ( f ) MDA-MB-231 cells exposed to ESE-one, ( g ) MCF-7 cells exposed to tiron only, ( h ) MCF-7 cells exposed to trolox only, ( i ) MCF-7 cells exposed to DMTU only, ( j ) MCF-7 cells coexposed to tiron and ESE-one, ( k ) MCF-7 cells coexposed to trolox and ESE-one, ( l ) MCF-7 cells coexposed to DMTU and ESE-one, ( m ) MDA-MB-231 cells exposed to tiron only, ( n ) MDA-MB-231 cells exposed to trolox only, ( o ) MDA-MB-231 cells exposed to DMTU only, ( p ) MDA-MB-231 cells coexposed to tiron and ESE-one, ( q ) MDA-MB-231 cells coexposed to trolox and ESE-one, ( r ) MDA-MB-231 cells coexposed to DMTU and ESE-one.

    Journal: Molecules

    Article Title: Dysregulation of Catalase by a Sulphamoylated Estradiol Analogue Culminates in Antimitotic Activity and Cell Death Induction in Breast Cancer Cell Lines

    doi: 10.3390/molecules26030622

    Figure Lengend Snippet: Cell cycle progression graphs of MCF-7 and MDA-MB-231 cells exposed to ESE-one in the presence or absence of various scavengers of reactive oxygen species (tiron, trolox and DMTU) for 48 h. ESE-one exposure resulted in a significant increase in the percentage of cells occupying the sub-G 1 phase in both MCF-7 and MDA-MB-231 cells. Tiron, trolox and DMTU exposure significantly decreased the number of cells occupying the sub-G 1 phase in MCF-7 cells and tiron as well as DMTU exposure significantly decreased the number of cells present in the sub-G 1 phase in MDA-MB-231 cells. Tiron, trolox and DMTU significantly decreased the number of cells in the G 2 /M phase in MDA-MB-231 cells and only tiron had a significant effect in MCF-7 cells. ( a ) MCF-7 cells cultured in complete growth medium, ( b ) vehicle-treated MCF-7 cells, ( c ) MCF-7 cells exposed to ESE-one only, ( d ) MDA-MB-231 cells cultured in complete growth medium, ( e ) vehicle-treated MDA-MB-231 cells, ( f ) MDA-MB-231 cells exposed to ESE-one, ( g ) MCF-7 cells exposed to tiron only, ( h ) MCF-7 cells exposed to trolox only, ( i ) MCF-7 cells exposed to DMTU only, ( j ) MCF-7 cells coexposed to tiron and ESE-one, ( k ) MCF-7 cells coexposed to trolox and ESE-one, ( l ) MCF-7 cells coexposed to DMTU and ESE-one, ( m ) MDA-MB-231 cells exposed to tiron only, ( n ) MDA-MB-231 cells exposed to trolox only, ( o ) MDA-MB-231 cells exposed to DMTU only, ( p ) MDA-MB-231 cells coexposed to tiron and ESE-one, ( q ) MDA-MB-231 cells coexposed to trolox and ESE-one, ( r ) MDA-MB-231 cells coexposed to DMTU and ESE-one.

    Article Snippet: Propidium iodide, trolox, DMTU and tiron were obtained from Sigma Chemical Co. (Sigma Chemical Co., St. Louis, MO, USA).

    Techniques: Multiple Displacement Amplification, Cell Culture

    Catalase activity graphs of MCF-7 and MDA-MB-231 cells exposed to ESE-one in the presence or absence of scavengers of reactive oxygen species (tiron, trolox and DMTU). Tiron and DMTU coexposure with ESE-one increased the catalase protein concentration significantly in MCF-7 cells and in MDA-MB-231 cells, tiron and trolox induced a significant increase in catalase protein concentration. ( a ) MCF-7, ( b ) MDA-MB-231. An asterisk (*) indicates p -value ( p

    Journal: Molecules

    Article Title: Dysregulation of Catalase by a Sulphamoylated Estradiol Analogue Culminates in Antimitotic Activity and Cell Death Induction in Breast Cancer Cell Lines

    doi: 10.3390/molecules26030622

    Figure Lengend Snippet: Catalase activity graphs of MCF-7 and MDA-MB-231 cells exposed to ESE-one in the presence or absence of scavengers of reactive oxygen species (tiron, trolox and DMTU). Tiron and DMTU coexposure with ESE-one increased the catalase protein concentration significantly in MCF-7 cells and in MDA-MB-231 cells, tiron and trolox induced a significant increase in catalase protein concentration. ( a ) MCF-7, ( b ) MDA-MB-231. An asterisk (*) indicates p -value ( p

    Article Snippet: Propidium iodide, trolox, DMTU and tiron were obtained from Sigma Chemical Co. (Sigma Chemical Co., St. Louis, MO, USA).

    Techniques: Activity Assay, Multiple Displacement Amplification, Protein Concentration

    SOD inhibition graphs of ( a ) MCF-7 and ( b ) MDA-MB-231 cells exposed to ESE-one in the presence or absence of scavengers of reactive oxygen species (tiron, trolox and DMTU). Tiron, trolox and DMTU coexposure with ESE-one resulted in an increased SOD inhibition percentage compared to ESE-one only exposure in MCF-7 cells suggesting that increased generation of reactive oxygen species due to ESE-one exposure is not required for the inhibition of SOD. In MDA-MB-231, DMTU demonstrated a decrease in SOD inhibition percentage suggesting that hydrogen peroxide affects the superoxide anion activity. ( a ) MCF-7, ( b ) MDA-MB-231. An asterisk (*) indicates p -value ( p

    Journal: Molecules

    Article Title: Dysregulation of Catalase by a Sulphamoylated Estradiol Analogue Culminates in Antimitotic Activity and Cell Death Induction in Breast Cancer Cell Lines

    doi: 10.3390/molecules26030622

    Figure Lengend Snippet: SOD inhibition graphs of ( a ) MCF-7 and ( b ) MDA-MB-231 cells exposed to ESE-one in the presence or absence of scavengers of reactive oxygen species (tiron, trolox and DMTU). Tiron, trolox and DMTU coexposure with ESE-one resulted in an increased SOD inhibition percentage compared to ESE-one only exposure in MCF-7 cells suggesting that increased generation of reactive oxygen species due to ESE-one exposure is not required for the inhibition of SOD. In MDA-MB-231, DMTU demonstrated a decrease in SOD inhibition percentage suggesting that hydrogen peroxide affects the superoxide anion activity. ( a ) MCF-7, ( b ) MDA-MB-231. An asterisk (*) indicates p -value ( p

    Article Snippet: Propidium iodide, trolox, DMTU and tiron were obtained from Sigma Chemical Co. (Sigma Chemical Co., St. Louis, MO, USA).

    Techniques: Inhibition, Multiple Displacement Amplification, Activity Assay

    ROS scavenging capacity of H. inuloides metabolites and semisynthetic derivatives. C=NDGA (superoxide); pyruvate (H 2 O 2 ); DMTU (OH − ); GSH ( 1 O 2 ); ascorbic Ac. acid (HOCl). The results were analyzed by ANOVA. The Dunnett's Multiple Comparison test was used to compare outcomes between experimental and control group. * P

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Antioxidant Capacity of “Mexican Arnica” Heterotheca inuloides Cass Natural Products and Some Derivatives: Their Anti-Inflammatory Evaluation and Effect on C. elegans Life Span

    doi: 10.1155/2015/843237

    Figure Lengend Snippet: ROS scavenging capacity of H. inuloides metabolites and semisynthetic derivatives. C=NDGA (superoxide); pyruvate (H 2 O 2 ); DMTU (OH − ); GSH ( 1 O 2 ); ascorbic Ac. acid (HOCl). The results were analyzed by ANOVA. The Dunnett's Multiple Comparison test was used to compare outcomes between experimental and control group. * P

    Article Snippet: Sodium pyruvate, dimethyl thiourea (DMTU), nordihydroguaiaretic acid (NDGA), ascorbic acid, histidine, xylenol orange (FOX), 2,2-diphenyl-1-picrylhydrazyl (DPPH), dimethylsulfoxide (DMSO), N,N-dimethyl-4-nitrosoaniline (DMNA), catalase, xanthine, xanthine oxidase, nitroblue tetrazolium (NBT), dL-penicillamine 2-thiobarbituric acid (TBA), α -tocopherol, Folin and Ciocalteu's phenol reagent, 3,5-di-tert-4-butylhydroxytoluene (BHT), and 5-fluoro-2′-deoxyuridine were purchased from Sigma-Aldrich (Toluca, Mexico, or Sigma, St. Louis, MO).

    Techniques:

    Representative micrographs of renal histopathology in mice at 72 h after cisplatin administration. Tubular necrosis, dilatation, and hyaline cast were observed in the cisplatin group, and the DMTU treatment suppressed the severity of the tissue damage. Bar = 50 μm

    Journal: EJNMMI Research

    Article Title: Imaging of reactive oxygen species using [3H]hydromethidine in mice with cisplatin-induced nephrotoxicity

    doi: 10.1186/s13550-015-0116-0

    Figure Lengend Snippet: Representative micrographs of renal histopathology in mice at 72 h after cisplatin administration. Tubular necrosis, dilatation, and hyaline cast were observed in the cisplatin group, and the DMTU treatment suppressed the severity of the tissue damage. Bar = 50 μm

    Article Snippet: Mice of the cisplatin + DMTU group were intraperitoneally administered with DMTU (100-mg/kg body weight, Sigma-Aldrich, St. Louis, MO, USA) dissolved in saline at 10 mg/mL, 30 min prior to the cisplatin administration (30 mg/kg).

    Techniques: Histopathology, Mouse Assay