Journal: ACS Chemical Neuroscience

Article Title: Cellular Basis for the Olfactory Response to Nicotine

doi: 10.1021/cn900042c

Figure Lengend Snippet: Neither 20 μM mecamylamine nor 20 μM hexamethonium had an effect on responses to 3 mM S(−)-nicotine in rat OSNs (A,B) and human cultured olfactory cells (C,D). Mecamylamine also had no effect on nicotine responses in fresh human biopsy OSNs (E). DMPP (10 μM) did not stimulate nicotine-sensitive rat OSNs (F).

Article Snippet: Other compounds used in this study were as follows: S (−)-nicotine (Philip Morris, Richmond, VA, >99% by GC), R (+)-nicotine (Toronto Research Chemical Inc., North York, Canada, 97% by TLC, NMR and optical rotation), noncompetitive nicotinic acetylcholine receptor antagonist mecamylamine hydrochloride (20 μM), competitive nicotinic acetylcholine receptor antagonist hexamethonium chloride (20 μM), nicotinic acetylcholine receptor agonist dimethylphenylpiperazinium (DMPP, 10 μM), adenylate cyclase inhibitor MDL-12,330A hydrochloride (20 μM), phospholipase C antagonists U-73122 (20 μM) and neomycin (1 mM), sarcoplasmic reticulum Ca 2+ -ATPase inhibitor thapsigargin (200 nM, Calbiochem, San Diego, CA), adenylate cyclase activator forskolin, phosphodiesterase inhibitor isobutylmethylxanthine (IBMX), leupeptin, l -cysteine, papain, and DNase I (Roche Diagnostics, Indianapolis, IN).

Techniques: Cell Culture