dmem (thermo fisher)


Structured Review

Dmem, supplied by thermo fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dmem/product/thermo fisher
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Circulating Adrenomedullin Is Elevated in Gestational Diabetes and Its Role in Impaired Insulin Production by ò-Cells"
Article Title: Circulating Adrenomedullin Is Elevated in Gestational Diabetes and Its Role in Impaired Insulin Production by ò-Cells
Journal: The Journal of Clinical Endocrinology and Metabolism
doi: 10.1210/jc.2018-01119

Figure Legend Snippet: Signaling molecules involved in ADM actions on PDX1â ò -Lox5 cell aggregates. Aggregated ò -Lox-5 cells transduced with PDX1 on day 7 of culture in DMEM containing 4 mg/mL glucose were treated with (A) KT5720 (KT, 10 õM), (B) PD98059 (PD, 10 õM), or (C) wortmannin (Wort, 0.1 õM) for 30 min and then ADM (0.1 õM) for 24 h, and insulin concentrations in the cell culture media were determined using human insulin ELISA kit. Data are displayed as mean ñ SEM of the results from three separate experiments. Different letters indicate significant differences between groups ( P > 0.05).
Techniques Used: Transduction, Cell Culture, Enzyme-linked Immunosorbent Assay

Figure Legend Snippet: ADM inhibits insulin mRNA and secretion in PDX1â ò -Lox5 cell aggregates. (A) mRNA for insulin in monolayer (Mono) or aggregated (Aggr) ò -Lox-5 cells transduced with or without PDX1 on day 7 of culture in DMEM containing 4 mg/mL glucose was determined using real-time quantitative PCR and normalized to GAPDH mRNA expression. (B) Aggregated ò -Lox-5 cells transduced with PDX1 on day 7 of culture in DMEM containing 4 mg/mL glucose were treated with ADM in the presence or absence of ADM22-52 for 24 h, and mRNA for insulin in the cells was determined using real-time quantitative PCR and normalized to GAPDH mRNA expression. (C) Insulin concentration in cell culture medium from (B) was determined using a human insulin ELISA kit. Data are displayed as mean ñ SEM of the results from three separate experiments. Different letters indicate significant differences between groups ( P > 0.05).
Techniques Used: Transduction, Real-time Polymerase Chain Reaction, Expressing, Concentration Assay, Cell Culture, Enzyme-linked Immunosorbent Assay
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