dmem medium  (Thermo Fisher)


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    Name:
    MEM
    Description:
    MEM Minimum Essential Medium is one of the most commonly used of all cell culture media MEM can be used with a variety of suspension and adherent mammalian cells including HeLa BHK 21 293 HEP 2 HT 1080 MCF 7 fibroblasts and primary rat astrocytes We offer a variety of MEM modifications for a range of cell culture applications Find the right formulation using the media selector tool This MEM is modified as follows With Without • L glutamine • HEPES • Phenol Red The complete formulation is available Using MEMMEM was developed by Harry Eagle based on his earlier formulation of Basal Medium Eagle BME Many other modifications of MEM followed including Glasgow s MEM MEM α DMEM and Temin s Modification MEM is available with Earle s salts for use in a CO2 incubator or with Hanks salts for use without CO2 This product is made with Earle s salts MEM contains no proteins lipids or growth factors Therefore MEM requires supplementation commonly with 10 Fetal Bovine Serum FBS MEM uses a sodium bicarbonate buffer system 2 2 g L and therefore requires a 5 10 CO2 environment to maintain physiological pH cGMP manufacturing and quality systemMEM is manufactured at a cGMP compliant facility located in Grand Island New York The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards For supply chain continuity we offer an identical MEM product made in our Scotland facility 31095 029 This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard
    Catalog Number:
    11095072
    Price:
    None
    Category:
    Cell Culture Transfection Reagents
    Applications:
    Cell Culture|Mammalian Cell Culture
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    Structured Review

    Thermo Fisher dmem medium
    MEM Minimum Essential Medium is one of the most commonly used of all cell culture media MEM can be used with a variety of suspension and adherent mammalian cells including HeLa BHK 21 293 HEP 2 HT 1080 MCF 7 fibroblasts and primary rat astrocytes We offer a variety of MEM modifications for a range of cell culture applications Find the right formulation using the media selector tool This MEM is modified as follows With Without • L glutamine • HEPES • Phenol Red The complete formulation is available Using MEMMEM was developed by Harry Eagle based on his earlier formulation of Basal Medium Eagle BME Many other modifications of MEM followed including Glasgow s MEM MEM α DMEM and Temin s Modification MEM is available with Earle s salts for use in a CO2 incubator or with Hanks salts for use without CO2 This product is made with Earle s salts MEM contains no proteins lipids or growth factors Therefore MEM requires supplementation commonly with 10 Fetal Bovine Serum FBS MEM uses a sodium bicarbonate buffer system 2 2 g L and therefore requires a 5 10 CO2 environment to maintain physiological pH cGMP manufacturing and quality systemMEM is manufactured at a cGMP compliant facility located in Grand Island New York The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards For supply chain continuity we offer an identical MEM product made in our Scotland facility 31095 029 This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard
    https://www.bioz.com/result/dmem medium/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dmem medium - by Bioz Stars, 2021-05
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: The PK Domain of the Large Subunit of Herpes Simplex Virus Type 2 Ribonucleotide Reductase (ICP10) Is Required for Immediate-Early Gene Expression and Virus Growth
    Article Snippet: .. They were cultured in EMEM with 10% FCS, 1 mM Na pyruvate (GIBCO-BRL, Gaithersburg, Md.), 1× nonessential amino acids (GIBCO-BRL), and antibiotics. .. Vero-ICP10 cells were derived by transfection of Vero cells with an ICP10 expression vector that has an SV2 - neo cassette (pJW17N) ( ).

    Article Title: High throughput discovery of influenza virus neutralizing antibodies from phage-displayed synthetic antibody libraries
    Article Snippet: With continuing advancement in designing and constructing synthetic scFv libraries containing diverse functional antibody fragments, this technological platform not only provides a promising option in mitigating the threats of IAV pandemics; the technology can also provide options to develop recombinant antibody candidates for passive immunotherapy treating other newly emerging viral infections. .. Cell lines MDCK (Madin-Darby canine kidney, ATCC CCL-34) epithelial cells cultured in MEM medium (Gibco, 11095080) supplemented with NEAA (non-essential amino acids, Gibco, 11140076), 2 mM L-glutamine, and 10% fetal bovine serum at 37 °C in a 5% CO2 humidified atmosphere incubator were used to determine microneutralization ability and IC50 in this study. .. 293T cells (ATCC CRL-3216) were cultured in DMEM medium (Gibco, 11965092) supplemented with 10% fetal bovine serum (Gibco, 10437028), penicillin-streptomycin (100×; Gibco, 15140122).

    Transfection:

    Article Title: UVA Induced Oxidative Stress Was Inhibited by Paeoniflorin/Nrf2 Signaling or PLIN2
    Article Snippet: Subsequently, the siRNA/RNAiMAX mixture was added to the cells in the 6-well plates with 1.8-ml Opti-MEM. .. The transfection medium was replaced with DMEM after incubation for 6 h. The transfection efficiency was detected by RT-qPCR and western blot at 24, 48, and 72 h after transfection. .. Two specific siRNA sequence were shown as follows: Nrf2 siRNA1 - rArUrUrGrArUrGrUrUrUrCrUrGrArUrCrUrArUrCrArCrUTT.

    Article Title: Golgi Calcium Pump Secretory Pathway Calcium ATPase 1 (SPCA1) Is a Key Regulator of Insulin-like Growth Factor Receptor (IGF1R) Processing in the Basal-like Breast Cancer Cell Line MDA-MB-231 *
    Article Snippet: MDA-MB-231 cells were seeded at 4000 cells/well into a 96-well plate and transfected with siRNA. .. Transfection medium was removed 72 h post-transfection, and cells were loaded with Fluo-4 AM (100 μl; 2 μ m ; Molecular Probes) in physiological salt solution, 1.8 m m Ca2+ , with PBX signal enhancer (PBX calcium assay kit; BD Biosciences) and 500 μ m probenecid for 1 h at 37 °C, 5% CO2 . .. Loading solution was removed and replaced with room temperature nominal calcium physiological salt solution (100 μl), PBX signal enhancer solution with 500 μ m probenecid, and the plate was placed in the fluorescence imaging plate reader (FLIPRTETRA , Molecular Devices).

    Article Title: Honey Bee Allatostatins Target Galanin/Somatostatin-Like Receptors and Modulate Learning: A Conserved Function?
    Article Snippet: Transfections with pRK5-ApimeAstC-R , pRK5-ApimeAstA-R or empty pRK5 vector were carried out in 15 ml Falcon tubes. .. Transfection medium for HEK293 cells was prepared using the Lipofectamine LTX kit (Invitrogen) with 2.5 ml Opti-MEM® (Gibco), 12.5 μl Plus™ Reagent and 4 μg of ApimeASTC-R (or ApimeASTA-R, or empty) expression construct, and 2 μg of CRE(6x)-Luc plasmid [ ]. .. The luciferase ORF, downstream of six tandem repeats of a cyclic AMP responsive element (CRE), in front of a minimum collate promoter, was used as a reporter gene (this reporter plasmid has been used by others [ – ])

    Incubation:

    Article Title: UVA Induced Oxidative Stress Was Inhibited by Paeoniflorin/Nrf2 Signaling or PLIN2
    Article Snippet: Subsequently, the siRNA/RNAiMAX mixture was added to the cells in the 6-well plates with 1.8-ml Opti-MEM. .. The transfection medium was replaced with DMEM after incubation for 6 h. The transfection efficiency was detected by RT-qPCR and western blot at 24, 48, and 72 h after transfection. .. Two specific siRNA sequence were shown as follows: Nrf2 siRNA1 - rArUrUrGrArUrGrUrUrUrCrUrGrArUrCrUrArUrCrArCrUTT.

    Quantitative RT-PCR:

    Article Title: UVA Induced Oxidative Stress Was Inhibited by Paeoniflorin/Nrf2 Signaling or PLIN2
    Article Snippet: Subsequently, the siRNA/RNAiMAX mixture was added to the cells in the 6-well plates with 1.8-ml Opti-MEM. .. The transfection medium was replaced with DMEM after incubation for 6 h. The transfection efficiency was detected by RT-qPCR and western blot at 24, 48, and 72 h after transfection. .. Two specific siRNA sequence were shown as follows: Nrf2 siRNA1 - rArUrUrGrArUrGrUrUrUrCrUrGrArUrCrUrArUrCrArCrUTT.

    Western Blot:

    Article Title: UVA Induced Oxidative Stress Was Inhibited by Paeoniflorin/Nrf2 Signaling or PLIN2
    Article Snippet: Subsequently, the siRNA/RNAiMAX mixture was added to the cells in the 6-well plates with 1.8-ml Opti-MEM. .. The transfection medium was replaced with DMEM after incubation for 6 h. The transfection efficiency was detected by RT-qPCR and western blot at 24, 48, and 72 h after transfection. .. Two specific siRNA sequence were shown as follows: Nrf2 siRNA1 - rArUrUrGrArUrGrUrUrUrCrUrGrArUrCrUrArUrCrArCrUTT.

    Calcium Assay:

    Article Title: Golgi Calcium Pump Secretory Pathway Calcium ATPase 1 (SPCA1) Is a Key Regulator of Insulin-like Growth Factor Receptor (IGF1R) Processing in the Basal-like Breast Cancer Cell Line MDA-MB-231 *
    Article Snippet: MDA-MB-231 cells were seeded at 4000 cells/well into a 96-well plate and transfected with siRNA. .. Transfection medium was removed 72 h post-transfection, and cells were loaded with Fluo-4 AM (100 μl; 2 μ m ; Molecular Probes) in physiological salt solution, 1.8 m m Ca2+ , with PBX signal enhancer (PBX calcium assay kit; BD Biosciences) and 500 μ m probenecid for 1 h at 37 °C, 5% CO2 . .. Loading solution was removed and replaced with room temperature nominal calcium physiological salt solution (100 μl), PBX signal enhancer solution with 500 μ m probenecid, and the plate was placed in the fluorescence imaging plate reader (FLIPRTETRA , Molecular Devices).

    Expressing:

    Article Title: Honey Bee Allatostatins Target Galanin/Somatostatin-Like Receptors and Modulate Learning: A Conserved Function?
    Article Snippet: Transfections with pRK5-ApimeAstC-R , pRK5-ApimeAstA-R or empty pRK5 vector were carried out in 15 ml Falcon tubes. .. Transfection medium for HEK293 cells was prepared using the Lipofectamine LTX kit (Invitrogen) with 2.5 ml Opti-MEM® (Gibco), 12.5 μl Plus™ Reagent and 4 μg of ApimeASTC-R (or ApimeASTA-R, or empty) expression construct, and 2 μg of CRE(6x)-Luc plasmid [ ]. .. The luciferase ORF, downstream of six tandem repeats of a cyclic AMP responsive element (CRE), in front of a minimum collate promoter, was used as a reporter gene (this reporter plasmid has been used by others [ – ])

    Construct:

    Article Title: Honey Bee Allatostatins Target Galanin/Somatostatin-Like Receptors and Modulate Learning: A Conserved Function?
    Article Snippet: Transfections with pRK5-ApimeAstC-R , pRK5-ApimeAstA-R or empty pRK5 vector were carried out in 15 ml Falcon tubes. .. Transfection medium for HEK293 cells was prepared using the Lipofectamine LTX kit (Invitrogen) with 2.5 ml Opti-MEM® (Gibco), 12.5 μl Plus™ Reagent and 4 μg of ApimeASTC-R (or ApimeASTA-R, or empty) expression construct, and 2 μg of CRE(6x)-Luc plasmid [ ]. .. The luciferase ORF, downstream of six tandem repeats of a cyclic AMP responsive element (CRE), in front of a minimum collate promoter, was used as a reporter gene (this reporter plasmid has been used by others [ – ])

    Plasmid Preparation:

    Article Title: Honey Bee Allatostatins Target Galanin/Somatostatin-Like Receptors and Modulate Learning: A Conserved Function?
    Article Snippet: Transfections with pRK5-ApimeAstC-R , pRK5-ApimeAstA-R or empty pRK5 vector were carried out in 15 ml Falcon tubes. .. Transfection medium for HEK293 cells was prepared using the Lipofectamine LTX kit (Invitrogen) with 2.5 ml Opti-MEM® (Gibco), 12.5 μl Plus™ Reagent and 4 μg of ApimeASTC-R (or ApimeASTA-R, or empty) expression construct, and 2 μg of CRE(6x)-Luc plasmid [ ]. .. The luciferase ORF, downstream of six tandem repeats of a cyclic AMP responsive element (CRE), in front of a minimum collate promoter, was used as a reporter gene (this reporter plasmid has been used by others [ – ])

    Modification:

    Article Title: Chorioallantoic Membrane Tumor Model for Evaluating Oncolytic Viruses
    Article Snippet: Cell lines and cell culture A549 (ATCC CCL-185, split rate 1:8), A431 (ATCC CRL1555, split rate 1:8), SK-Mel-28 (ATCC HTB-72, split rate 1:3), and UM-SCC-11B (obtained from Prof. C. Brunner, University clinic Ulm, split rate 1:8) were passaged twice a week. .. Except for A549, which were cultivated in Minimum Essential Medium (MEM) (31095-029; Gibco), cell lines were cultivated in Dulbecco's Modified Eagle Medium (DMEM) (10938-025; Gibco) on tissue culture plates (83.3903; Sarstedt). .. Cells were incubated at 90% humidity, 5% CO2, and 37°C in cell culture medium supplemented with 10% fetal calf serum (10270-106; Gibco) and 1% penicillin/streptomycin/glutamine (10378-016; Gibco).

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  • 86
    Thermo Fisher dulbecco s modified eagle s medium
    (A) The expression of elastin (ELN, green) colocalized with the tenocytes as labelled by tenascin-C (TN-C, red) in normal tendon (incubated in DMEM). ELNase specifically degrades ELN but does not disrupt the fascicle, as illustrated in the TN-C staining. However, COLase caused severe diffusion of the ELN and TN-C expressions. (B) The increase in COL production during ELNase incubation is confirmed by specific immunofluorescence staining of COL. (C) Both ELNase and COLase increased the cyclooxygenase-2 (COX-2) expressions to induce the inflammation in tenocytes. Scale bar ​= ​50 ​μm. DMEM = <t>Dulbecco's</t> Modified <t>Eagle's</t> Medium.
    Dulbecco S Modified Eagle S Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dulbecco s modified eagle s medium/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    99
    Thermo Fisher n2b27 medium
    Oct4-low iPSCs self-renew in the absence of pluripotent culture requisites. ( a ) Phase images of PB-Oct4 iPSCs −/− treated or untreated with 4OHT for 24 h and subsequently cultured in <t>N2B27</t> conditions with selection for geneticin (G418)
    N2b27 Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n2b27 medium/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
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    n2b27 medium - by Bioz Stars, 2021-05
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    99
    Thermo Fisher dulbecco s modified eagle s medium dmem
    Rosiglitazone abolishes selenate-inhibited adipogenesis. After a 24 h pretreatment of one-day post-confluent 3T3-L1 preadipocytes with 50 µM of selenate, the cell differentiation was initiated by the addition of an adipogenic cocktail with 0 or 1 µM rosiglitazone during Day 0–2, in the absence of selenate. On Day 2, the cells were transferred to <t>Dulbecco’s</t> modified <t>Eagle’s</t> medium <t>(DMEM)</t> containing 10% fetal bovine serum (FBS-DMEM) and insulin, and to 10% FBS-DMEM on Day 4–6. At Day 6, Oil Red O staining was performed ( A ). The quantitative analysis of staining is shown in ( B ). The data represent mean ± SEM. The experiment was conducted three times ( n = 3–9). * p
    Dulbecco S Modified Eagle S Medium Dmem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dulbecco s modified eagle s medium dmem/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dulbecco s modified eagle s medium dmem - by Bioz Stars, 2021-05
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    Image Search Results


    (A) The expression of elastin (ELN, green) colocalized with the tenocytes as labelled by tenascin-C (TN-C, red) in normal tendon (incubated in DMEM). ELNase specifically degrades ELN but does not disrupt the fascicle, as illustrated in the TN-C staining. However, COLase caused severe diffusion of the ELN and TN-C expressions. (B) The increase in COL production during ELNase incubation is confirmed by specific immunofluorescence staining of COL. (C) Both ELNase and COLase increased the cyclooxygenase-2 (COX-2) expressions to induce the inflammation in tenocytes. Scale bar ​= ​50 ​μm. DMEM = Dulbecco's Modified Eagle's Medium.

    Journal: Journal of Orthopaedic Translation

    Article Title: Sequential inflammation model for Achilles tendinopathy by elastin degradation with treadmill exercise

    doi: 10.1016/j.jot.2020.03.004

    Figure Lengend Snippet: (A) The expression of elastin (ELN, green) colocalized with the tenocytes as labelled by tenascin-C (TN-C, red) in normal tendon (incubated in DMEM). ELNase specifically degrades ELN but does not disrupt the fascicle, as illustrated in the TN-C staining. However, COLase caused severe diffusion of the ELN and TN-C expressions. (B) The increase in COL production during ELNase incubation is confirmed by specific immunofluorescence staining of COL. (C) Both ELNase and COLase increased the cyclooxygenase-2 (COX-2) expressions to induce the inflammation in tenocytes. Scale bar ​= ​50 ​μm. DMEM = Dulbecco's Modified Eagle's Medium.

    Article Snippet: To understand the ECM degradation in tendon structures, the fresh, isolated Achilles tendon samples were immediately incubated with ELNase (1 U/mL; Sigma-Aldrich, Inc.) or COLase (COLase type I, Cat# 17018029, 5 ​mg/mL; Invitrogen, Thermo Fisher Scientific Co.) in Dulbecco's Modified Eagle's Medium (DMEM; Invitrogen) at 37 ​°C for 12 ​h.

    Techniques: Expressing, Incubation, Staining, Diffusion-based Assay, Immunofluorescence, Modification

    Validation of the enzyme-linked immunosorbent assay (ELISA). PA-EAAT2 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) and treated with retinoic acid (1.5 μg/mL) for 72 h and then subjected to ( A ) ELISA, ( B ) Western

    Journal: Journal of biomolecular screening

    Article Title: Identification of Translational Activators of Glial Glutamate Transporter EAAT2 through Cell-Based High-Throughput Screening: An Approach to Prevent Excitotoxicity

    doi: 10.1177/1087057110370998

    Figure Lengend Snippet: Validation of the enzyme-linked immunosorbent assay (ELISA). PA-EAAT2 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) and treated with retinoic acid (1.5 μg/mL) for 72 h and then subjected to ( A ) ELISA, ( B ) Western

    Article Snippet: PA-EAAT2 cells at passage 9 were thawed from frozen stocks and grown in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen, Carlsbad, CA) containing 25 mM glucose, 1 mM sodium pyruvate, 19.4 μM pyridoxine hydrochloride, and 2 mM glutamine and supplemented with 10% fetal bovine serum (FBS), 700 μg/mL geneticin (Gibco, Los Angeles, CA), and 100 μg/mL penicillin-streptomycin (Sigma, St. Louis, MO).

    Techniques: Enzyme-linked Immunosorbent Assay, Cell Culture, Modification, Western Blot

    Confirmation of the hits. PA-EAAT2 cells were treated with indicated concentrations of compound 9 in Dulbecco’s modified Eagle’s medium (DMEM) for 72 h and then harvested ( A ) for determining EAAT2 protein levels by Western blotting (equal

    Journal: Journal of biomolecular screening

    Article Title: Identification of Translational Activators of Glial Glutamate Transporter EAAT2 through Cell-Based High-Throughput Screening: An Approach to Prevent Excitotoxicity

    doi: 10.1177/1087057110370998

    Figure Lengend Snippet: Confirmation of the hits. PA-EAAT2 cells were treated with indicated concentrations of compound 9 in Dulbecco’s modified Eagle’s medium (DMEM) for 72 h and then harvested ( A ) for determining EAAT2 protein levels by Western blotting (equal

    Article Snippet: PA-EAAT2 cells at passage 9 were thawed from frozen stocks and grown in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen, Carlsbad, CA) containing 25 mM glucose, 1 mM sodium pyruvate, 19.4 μM pyridoxine hydrochloride, and 2 mM glutamine and supplemented with 10% fetal bovine serum (FBS), 700 μg/mL geneticin (Gibco, Los Angeles, CA), and 100 μg/mL penicillin-streptomycin (Sigma, St. Louis, MO).

    Techniques: Modification, Western Blot

    Oct4-low iPSCs self-renew in the absence of pluripotent culture requisites. ( a ) Phase images of PB-Oct4 iPSCs −/− treated or untreated with 4OHT for 24 h and subsequently cultured in N2B27 conditions with selection for geneticin (G418)

    Journal: Nature cell biology

    Article Title: A defined Oct4 level governs cell state transitions of pluripotency entry and differentiation into all embryonic lineages

    doi: 10.1038/ncb2742

    Figure Lengend Snippet: Oct4-low iPSCs self-renew in the absence of pluripotent culture requisites. ( a ) Phase images of PB-Oct4 iPSCs −/− treated or untreated with 4OHT for 24 h and subsequently cultured in N2B27 conditions with selection for geneticin (G418)

    Article Snippet: Epiblast stem cells were cultured in N2B27 medium (DMEM/F12 and Neurobasal (both Gibco) in a 1:1 ratio, 1× penicillin/streptomycin, 0.1 mM 2-mercaptoethanol, 2 mM l -glutamine, 1:200 N2 (StemCells) and 1:100 B27 supplement) supplemented with 12 ng ml−1 Fgf2 and 20 ng ml−1 Activin A (home-made).

    Techniques: Cell Culture, Selection

    Rosiglitazone abolishes selenate-inhibited adipogenesis. After a 24 h pretreatment of one-day post-confluent 3T3-L1 preadipocytes with 50 µM of selenate, the cell differentiation was initiated by the addition of an adipogenic cocktail with 0 or 1 µM rosiglitazone during Day 0–2, in the absence of selenate. On Day 2, the cells were transferred to Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS-DMEM) and insulin, and to 10% FBS-DMEM on Day 4–6. At Day 6, Oil Red O staining was performed ( A ). The quantitative analysis of staining is shown in ( B ). The data represent mean ± SEM. The experiment was conducted three times ( n = 3–9). * p

    Journal: Molecules

    Article Title: Selenate Prevents Adipogenesis through Induction of Selenoprotein S and Attenuation of Endoplasmic Reticulum Stress

    doi: 10.3390/molecules23112882

    Figure Lengend Snippet: Rosiglitazone abolishes selenate-inhibited adipogenesis. After a 24 h pretreatment of one-day post-confluent 3T3-L1 preadipocytes with 50 µM of selenate, the cell differentiation was initiated by the addition of an adipogenic cocktail with 0 or 1 µM rosiglitazone during Day 0–2, in the absence of selenate. On Day 2, the cells were transferred to Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS-DMEM) and insulin, and to 10% FBS-DMEM on Day 4–6. At Day 6, Oil Red O staining was performed ( A ). The quantitative analysis of staining is shown in ( B ). The data represent mean ± SEM. The experiment was conducted three times ( n = 3–9). * p

    Article Snippet: The Dulbecco’s Modified Eagle’s medium (DMEM) and 0.25% trypsin-EDTA were obtained from Thermo Fisher Scientific (Waltham, MA, USA).

    Techniques: Cell Differentiation, Modification, Staining