dmem ko  (Thermo Fisher)


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    Name:
    DMEM F 12 no glutamine
    Description:
    Dulbecco s Modified Eagle Medium Nutrient Mixture F 12 DMEM F 12 is a widely used basal medium for supporting the growth of many different mammalian cells Cells successfully cultured in DMEM F 12 include MDCK glial cells fibroblasts human endothelial cells and rat fibroblasts We offer a variety of Gibco DMEM F 12 modifications for a range of cell culture applications Find the right formulation using the media selector tool This DMEM F 12 is modified as follows WithWithoutPhenol RedL glutamineHEPESThe complete formulation is available Gibco DMEM F 12 is a 1 1 mixture of DMEM and Ham s F 12 This formulation combines DMEM s high concentrations of glucose amino acids and vitamins with F 12 s wide variety of components Product UseFor in vitro diagnostic use CAUTION Not for human or animal therapeutic use Uses other than the intended use may be a violation of local law cGMP Manufacturing and Quality SystemGibco DMEM F 12 is manufactured at a cGMP compliant facility located in Paisley Scotland UK The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard DMEM F 12 contains no proteins lipids or growth factors Therefore DMEM F 12 may require supplementation commonly with 10 Fetal Bovine Serum FBS DMEM F 12 uses a sodium bicarbonate buffer system and therefore requires a 5 10 CO2 environment to maintain physiological pH
    Catalog Number:
    21331020
    Price:
    None
    Category:
    Cell Culture Transfection Reagents
    Applications:
    Cell Culture|Mammalian Cell Culture
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    Structured Review

    Thermo Fisher dmem ko
    Tri-lineage differentiation. A-I) Representative images of XF,SFM-cultured and <t>DMEM</t> KO + 10% <t>FBS-cultured</t> MSCs, differentiated into adipocytes, osteocytes and chondrocytes, as visualized by Oil Red O, von Kossa and alcian blue stains, respectively, against undifferentiated controls; J) Bar graph to quantify oil droplets, post Oil Red O staining; K) Bar graph to quantify mineralization, post alizarin red staining; L) Bar graph to quantify sulfated glycosaminoglycan production by differentiated chondrocytes, normalized to DNA content. FBS, fetal bovine serum; MSCs, mesenchymal stem cells.
    Dulbecco s Modified Eagle Medium Nutrient Mixture F 12 DMEM F 12 is a widely used basal medium for supporting the growth of many different mammalian cells Cells successfully cultured in DMEM F 12 include MDCK glial cells fibroblasts human endothelial cells and rat fibroblasts We offer a variety of Gibco DMEM F 12 modifications for a range of cell culture applications Find the right formulation using the media selector tool This DMEM F 12 is modified as follows WithWithoutPhenol RedL glutamineHEPESThe complete formulation is available Gibco DMEM F 12 is a 1 1 mixture of DMEM and Ham s F 12 This formulation combines DMEM s high concentrations of glucose amino acids and vitamins with F 12 s wide variety of components Product UseFor in vitro diagnostic use CAUTION Not for human or animal therapeutic use Uses other than the intended use may be a violation of local law cGMP Manufacturing and Quality SystemGibco DMEM F 12 is manufactured at a cGMP compliant facility located in Paisley Scotland UK The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard DMEM F 12 contains no proteins lipids or growth factors Therefore DMEM F 12 may require supplementation commonly with 10 Fetal Bovine Serum FBS DMEM F 12 uses a sodium bicarbonate buffer system and therefore requires a 5 10 CO2 environment to maintain physiological pH
    https://www.bioz.com/result/dmem ko/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dmem ko - by Bioz Stars, 2021-03
    97/100 stars

    Images

    1) Product Images from "Are serum-free and xeno-free culture conditions ideal for large scale clinical grade expansion of Wharton’s jelly derived mesenchymal stem cells? A comparative study"

    Article Title: Are serum-free and xeno-free culture conditions ideal for large scale clinical grade expansion of Wharton’s jelly derived mesenchymal stem cells? A comparative study

    Journal: Stem Cell Research & Therapy

    doi: 10.1186/scrt477

    Tri-lineage differentiation. A-I) Representative images of XF,SFM-cultured and DMEM KO + 10% FBS-cultured MSCs, differentiated into adipocytes, osteocytes and chondrocytes, as visualized by Oil Red O, von Kossa and alcian blue stains, respectively, against undifferentiated controls; J) Bar graph to quantify oil droplets, post Oil Red O staining; K) Bar graph to quantify mineralization, post alizarin red staining; L) Bar graph to quantify sulfated glycosaminoglycan production by differentiated chondrocytes, normalized to DNA content. FBS, fetal bovine serum; MSCs, mesenchymal stem cells.
    Figure Legend Snippet: Tri-lineage differentiation. A-I) Representative images of XF,SFM-cultured and DMEM KO + 10% FBS-cultured MSCs, differentiated into adipocytes, osteocytes and chondrocytes, as visualized by Oil Red O, von Kossa and alcian blue stains, respectively, against undifferentiated controls; J) Bar graph to quantify oil droplets, post Oil Red O staining; K) Bar graph to quantify mineralization, post alizarin red staining; L) Bar graph to quantify sulfated glycosaminoglycan production by differentiated chondrocytes, normalized to DNA content. FBS, fetal bovine serum; MSCs, mesenchymal stem cells.

    Techniques Used: Cell Culture, Staining

    Growth kinetics and CFU-F efficacy. A-F) Representative morphology pictures of WJ derived MSCs cultured in DMEM KO + 10% FBS and MesenCult XF,SF media; G,H,I) Total cell yield obtained at passage 5 using XF,SF medium, compared against DMEM KO + 10% FBS; K) Average number of colonies generated per 100 cells at passage 3 and passage 4, by cells cultured in XF,SF media and DMEM KO + 10% FBS; J,L) Individual colonies captured at 4X magnification, for XF,SFM cultures and DMEM KO + 10% FBS cultures, respectively. FBS, fetal bovine serum; GFU-F, colony-forming unit-fibroblast; MSCs, mesenchymal stem cells; WJ, Wharton’s jelly.
    Figure Legend Snippet: Growth kinetics and CFU-F efficacy. A-F) Representative morphology pictures of WJ derived MSCs cultured in DMEM KO + 10% FBS and MesenCult XF,SF media; G,H,I) Total cell yield obtained at passage 5 using XF,SF medium, compared against DMEM KO + 10% FBS; K) Average number of colonies generated per 100 cells at passage 3 and passage 4, by cells cultured in XF,SF media and DMEM KO + 10% FBS; J,L) Individual colonies captured at 4X magnification, for XF,SFM cultures and DMEM KO + 10% FBS cultures, respectively. FBS, fetal bovine serum; GFU-F, colony-forming unit-fibroblast; MSCs, mesenchymal stem cells; WJ, Wharton’s jelly.

    Techniques Used: Derivative Assay, Cell Culture, Generated

    Senescence, apoptosis and transformation. A) Percentage of senescent cells in XF,SFM cultures, compared against DMEM KO + 10% FBS cultures at P5 followed by representative 10 X images of fields showing blue colored senescent cells; B) Percentage of Annexin positive (apoptotic) cells in XF,SFM cultures compared to DMEM KO + 10% FBS cultures at P5 followed by representative images of fields showing green colored apoptotic cells; C) Representative pictures of cells cultured in soft agar medium, showing no colonies in XF,SFM and DMEM KO + 10% FBS cultures, compared to colonies observed in MCF-7 cultures; D) Comparative gene expression analysis between cells cultured in XF,SF medium and DMEM KO + 10% FBS at P6. FBS, fetal bovine serum; P, passage.
    Figure Legend Snippet: Senescence, apoptosis and transformation. A) Percentage of senescent cells in XF,SFM cultures, compared against DMEM KO + 10% FBS cultures at P5 followed by representative 10 X images of fields showing blue colored senescent cells; B) Percentage of Annexin positive (apoptotic) cells in XF,SFM cultures compared to DMEM KO + 10% FBS cultures at P5 followed by representative images of fields showing green colored apoptotic cells; C) Representative pictures of cells cultured in soft agar medium, showing no colonies in XF,SFM and DMEM KO + 10% FBS cultures, compared to colonies observed in MCF-7 cultures; D) Comparative gene expression analysis between cells cultured in XF,SF medium and DMEM KO + 10% FBS at P6. FBS, fetal bovine serum; P, passage.

    Techniques Used: Transformation Assay, Cell Culture, Expressing

    Immunomodulation, karyotype and cell cycle analysis. A) Bar graph showing percentage suppression of PBMC-generated MLRs, by cells cultured in XF,SF medium, against those cultured in DMEM KO + 10% FBS, at passages 4 and 6; B) Percentage proliferation brought about by co-culturing MSCs (cultured in both conditions) with human peripheral blood-derived PBMCs, at passages 4 and 6, shown against a one-way mixed lymphocyte reaction taken as 100% proliferation; C and D) Representative karyotypes of cells cultured in XF,SF medium and DMEM KO + 10% FBS, respectively, at passage 5; E) Percentage of cells cultured in XF,SFM and DMEM KO + 10% FBS, in S phase at passage 5; F) Percentage of cells cultured in XF,SF medium and DMEM KO + 10% FBS in G0/G1, S and M phases, at passage 5. FBS, fetal bovine serum; PBMC, peripheral blood mononuclear cells.
    Figure Legend Snippet: Immunomodulation, karyotype and cell cycle analysis. A) Bar graph showing percentage suppression of PBMC-generated MLRs, by cells cultured in XF,SF medium, against those cultured in DMEM KO + 10% FBS, at passages 4 and 6; B) Percentage proliferation brought about by co-culturing MSCs (cultured in both conditions) with human peripheral blood-derived PBMCs, at passages 4 and 6, shown against a one-way mixed lymphocyte reaction taken as 100% proliferation; C and D) Representative karyotypes of cells cultured in XF,SF medium and DMEM KO + 10% FBS, respectively, at passage 5; E) Percentage of cells cultured in XF,SFM and DMEM KO + 10% FBS, in S phase at passage 5; F) Percentage of cells cultured in XF,SF medium and DMEM KO + 10% FBS in G0/G1, S and M phases, at passage 5. FBS, fetal bovine serum; PBMC, peripheral blood mononuclear cells.

    Techniques Used: Cell Cycle Assay, Generated, Cell Culture, Derivative Assay

    Angiogenic potency. A, B) Real-time PCR quantification of HGF, VEGF, TGFβ-1 and IL6 for cells cultured in DMEM KO + 10% FBS and in XF,SFM, all normalized against Cord 1 (DMEM KO + 10% FBS); C,D,E) Box graphs representing variations observed between cells cultured in XF,SFM and FBS-containing medium, when analyzed for cytokine secretions of TGFβ, HGF and Ang1; F) Tube formation efficiency (estimated using HUVEC, cultured on growth factor reduced Matrigel) of WJ-MSC-conditioned medium, from three cords cultured in DMEM KO + 10% FBS and in XF, SFM. Anti-HGF neutralizing antibody was used to block tube formation in each condition. G-J’) Representative 4 X pictures showing tubes formed. FBS, fetal bovine serum; HGF, hepatocyte growth factor; TGFβ1, transforming growth factor-β1; VEGF, vascular endothelial growth factor. ** signifies extent of significance of the numeric value.
    Figure Legend Snippet: Angiogenic potency. A, B) Real-time PCR quantification of HGF, VEGF, TGFβ-1 and IL6 for cells cultured in DMEM KO + 10% FBS and in XF,SFM, all normalized against Cord 1 (DMEM KO + 10% FBS); C,D,E) Box graphs representing variations observed between cells cultured in XF,SFM and FBS-containing medium, when analyzed for cytokine secretions of TGFβ, HGF and Ang1; F) Tube formation efficiency (estimated using HUVEC, cultured on growth factor reduced Matrigel) of WJ-MSC-conditioned medium, from three cords cultured in DMEM KO + 10% FBS and in XF, SFM. Anti-HGF neutralizing antibody was used to block tube formation in each condition. G-J’) Representative 4 X pictures showing tubes formed. FBS, fetal bovine serum; HGF, hepatocyte growth factor; TGFβ1, transforming growth factor-β1; VEGF, vascular endothelial growth factor. ** signifies extent of significance of the numeric value.

    Techniques Used: Real-time Polymerase Chain Reaction, Cell Culture, Blocking Assay

    2) Product Images from "A comprehensive study on optimization of proliferation and differentiation potency of bone marrow derived mesenchymal stem cells under prolonged culture condition"

    Article Title: A comprehensive study on optimization of proliferation and differentiation potency of bone marrow derived mesenchymal stem cells under prolonged culture condition

    Journal: Cytotechnology

    doi: 10.1007/s10616-012-9471-0

    Adipogenic differentiation of bone marrow derived MSC. Confirmation of adipogenesis using Oil Red O staining at early - P3 ( a – e ) and late passages - P20 ( f – j ) control: a , f ; DMEM-LG: b , g ; ALPHA-MEM: c , h ; DMEM-F12: d , i ; DMEM-KO: e , j )
    Figure Legend Snippet: Adipogenic differentiation of bone marrow derived MSC. Confirmation of adipogenesis using Oil Red O staining at early - P3 ( a – e ) and late passages - P20 ( f – j ) control: a , f ; DMEM-LG: b , g ; ALPHA-MEM: c , h ; DMEM-F12: d , i ; DMEM-KO: e , j )

    Techniques Used: Derivative Assay, Staining

    Population doubling time analysis of Bone marrow derived MSC. Population doubling time analysis of bone marrow derived MSC at early-P3 and late -P20 passage with respect to different media: DMEM-LG, α-MEM, DMEM-F12 and DMEM–KO
    Figure Legend Snippet: Population doubling time analysis of Bone marrow derived MSC. Population doubling time analysis of bone marrow derived MSC at early-P3 and late -P20 passage with respect to different media: DMEM-LG, α-MEM, DMEM-F12 and DMEM–KO

    Techniques Used: Derivative Assay

    Osteogenic differentiation of bone marrow derived MSC. Confirmation of osteogenesis using von Kossa staining at early - P3 ( a – e ) and late passages-P20 ( f – j ) control: a , f ; DMEM-LG: b , g ; ALPHA-MEM: c , h ; DMEM-F12: d , i ; DMEM-KO: e , j ) Alizarin
    Figure Legend Snippet: Osteogenic differentiation of bone marrow derived MSC. Confirmation of osteogenesis using von Kossa staining at early - P3 ( a – e ) and late passages-P20 ( f – j ) control: a , f ; DMEM-LG: b , g ; ALPHA-MEM: c , h ; DMEM-F12: d , i ; DMEM-KO: e , j ) Alizarin

    Techniques Used: Derivative Assay, Staining

    3) Product Images from "Human Adult Stem Cells Maintain a Constant Phenotype Profile Irrespective of Their Origin, Basal Media, and Long Term Cultures"

    Article Title: Human Adult Stem Cells Maintain a Constant Phenotype Profile Irrespective of Their Origin, Basal Media, and Long Term Cultures

    Journal: Stem Cells International

    doi: 10.1155/2015/146051

    Flowcytometric characterization of MSCs and CAM of omentum fat derived MSC. Characterization of omentum fat derived MSC at early (P3) and late (P20) passages in DMEM-LG, Alpha-MEM, DMEM-F12, and DMEM-KO for CD90, CD105, CD73, CD29, CD49d, CD44, CD166, CD106, CD54, and CD31 using flowcytometry.
    Figure Legend Snippet: Flowcytometric characterization of MSCs and CAM of omentum fat derived MSC. Characterization of omentum fat derived MSC at early (P3) and late (P20) passages in DMEM-LG, Alpha-MEM, DMEM-F12, and DMEM-KO for CD90, CD105, CD73, CD29, CD49d, CD44, CD166, CD106, CD54, and CD31 using flowcytometry.

    Techniques Used: Chick Chorioallantoic Membrane Assay, Derivative Assay

    Flowcytometric characterization of HSC and unique markers of omentum fat derived MSC. Characterization of omentum fat derived MSC at early (P3) and late (P20) passages in DMEM-LG, Alpha-MEM, DMEM-F12, and DMEM-KO for CD34, CD45, CD133, HLA-DR, CD117, ABCG2, ALDH, SSEA4, and CD13 using flowcytometry.
    Figure Legend Snippet: Flowcytometric characterization of HSC and unique markers of omentum fat derived MSC. Characterization of omentum fat derived MSC at early (P3) and late (P20) passages in DMEM-LG, Alpha-MEM, DMEM-F12, and DMEM-KO for CD34, CD45, CD133, HLA-DR, CD117, ABCG2, ALDH, SSEA4, and CD13 using flowcytometry.

    Techniques Used: Derivative Assay

    Marker expression profiling of MSCs cultured in DMEM-F12. Comparative Surface antigenic profiling of MSCs derived from omentum fat, subcutaneous fat, and bone marrow at early (P3) and late (P20) passages cultured in DMEM-F12.
    Figure Legend Snippet: Marker expression profiling of MSCs cultured in DMEM-F12. Comparative Surface antigenic profiling of MSCs derived from omentum fat, subcutaneous fat, and bone marrow at early (P3) and late (P20) passages cultured in DMEM-F12.

    Techniques Used: Marker, Expressing, Cell Culture, Derivative Assay

    4) Product Images from "Expansion and characterization of bone marrow derived human mesenchymal stromal cells in serum-free conditions"

    Article Title: Expansion and characterization of bone marrow derived human mesenchymal stromal cells in serum-free conditions

    Journal: Scientific Reports

    doi: 10.1038/s41598-021-83088-1

    Morphology of BM-MSCs cultured at P4 stage. ( a1 , a2 , h1 , h2 ) BM-MSCs cultured in DMEM-KO + 10% FBS at a seeding density of 1000 cells/cm 2 ( a1 , a2 ) and 5000 cells/ cm 2 ( h1 , h2 ). ( a1 ) Microphotographs at 10–20% confluency, a2: 80–90% confluency, ( h1 ): 50–55% confluency and ( h2 ): 80–90% confluency. Similarly ( b1, b2, i1, i2 ) BM-MSCs cultured in RoosterNourish. c1, c2, j1, j2 - BM-MSCs cultured in RoosterNourish-MSC XF. d1, d2, k1, k2 - BM-MSCs cultured in StemMACS-MSC XF. ( e1, e2, i1, i2 ) BM-MSCs cultured in StemXVivo serum-free media. f1, f2, m1, m2 - BM-MSCs cultured in PLTMax human platelet lysate. ( g1, g2, n1, n2 ) BM-MSCs cultured in MSC NutriStem XF . ( b1, b2, c1, c2, d1, d2, e1, e2, f1, f2, g1, g2 ) MSC at a seeding density of 1000 cells/cm 2 and ( i1, i2, j1, j2, k1, k2, l1, l2, m1, m2, n1, n2 ) 5000 cells/cm 2 . ( b1, c1, d1, e1, f1, g1 ) Microphotographs at 20–25% confluency. ( b2, c2, d2, e2, f2, g2 ): 80–90% confluency. ( i1, j1, k1, l1, m1, n1 ): 50–55% confluency and ( i2, j2, k2, l2, m2, n2 ): 80–90% confluency. Experiments were performed in duplicates and the representative images are shown. The images were captured at 10× magnification with scale bar of ∼100 μm.
    Figure Legend Snippet: Morphology of BM-MSCs cultured at P4 stage. ( a1 , a2 , h1 , h2 ) BM-MSCs cultured in DMEM-KO + 10% FBS at a seeding density of 1000 cells/cm 2 ( a1 , a2 ) and 5000 cells/ cm 2 ( h1 , h2 ). ( a1 ) Microphotographs at 10–20% confluency, a2: 80–90% confluency, ( h1 ): 50–55% confluency and ( h2 ): 80–90% confluency. Similarly ( b1, b2, i1, i2 ) BM-MSCs cultured in RoosterNourish. c1, c2, j1, j2 - BM-MSCs cultured in RoosterNourish-MSC XF. d1, d2, k1, k2 - BM-MSCs cultured in StemMACS-MSC XF. ( e1, e2, i1, i2 ) BM-MSCs cultured in StemXVivo serum-free media. f1, f2, m1, m2 - BM-MSCs cultured in PLTMax human platelet lysate. ( g1, g2, n1, n2 ) BM-MSCs cultured in MSC NutriStem XF . ( b1, b2, c1, c2, d1, d2, e1, e2, f1, f2, g1, g2 ) MSC at a seeding density of 1000 cells/cm 2 and ( i1, i2, j1, j2, k1, k2, l1, l2, m1, m2, n1, n2 ) 5000 cells/cm 2 . ( b1, c1, d1, e1, f1, g1 ) Microphotographs at 20–25% confluency. ( b2, c2, d2, e2, f2, g2 ): 80–90% confluency. ( i1, j1, k1, l1, m1, n1 ): 50–55% confluency and ( i2, j2, k2, l2, m2, n2 ): 80–90% confluency. Experiments were performed in duplicates and the representative images are shown. The images were captured at 10× magnification with scale bar of ∼100 μm.

    Techniques Used: Cell Culture

    Related Articles

    Western Blot:

    Article Title: Nutrient-induced FNIP degradation by SCFβ-TRCP regulates FLCN complex localization and promotes renal cancer progression
    Article Snippet: .. Where indicated, HeLa, UOK-257, or UOK-257-2 cells were starved in Earle's Balanced Salt solution (Sigma-Aldrich) for 3 h and stimulated with 10% FBS, glucose-free or glutamine-free DMEM (Gibco) for a subsequent western blot or immunofluorescence analysis. .. Plasmids Flag-FNIP2 expression plasmid was described previously [ ].

    Immunofluorescence:

    Article Title: Nutrient-induced FNIP degradation by SCFβ-TRCP regulates FLCN complex localization and promotes renal cancer progression
    Article Snippet: .. Where indicated, HeLa, UOK-257, or UOK-257-2 cells were starved in Earle's Balanced Salt solution (Sigma-Aldrich) for 3 h and stimulated with 10% FBS, glucose-free or glutamine-free DMEM (Gibco) for a subsequent western blot or immunofluorescence analysis. .. Plasmids Flag-FNIP2 expression plasmid was described previously [ ].

    Staining:

    Article Title: Are serum-free and xeno-free culture conditions ideal for large scale clinical grade expansion of Wharton’s jelly derived mesenchymal stem cells? A comparative study
    Article Snippet: .. Annexin V staining We looked for the presence of apoptotic cells among cultures in DMEM KO + 10% FBS and MesenCult, using the Tali Apoptosis Kit – Annexin V Alexa Flour 488 and propidium iodide (PI; cat no. 10788; Invitrogen), according to the manufacturer’s instructions. .. After staining the cells with Annexin V Alexa Flour 488 and PI, the samples were loaded onto Tali Cellular Analysis Slides (cat no. T10794; Invitrogen), following which 20 images were captured using the Tali Image – Based Cytometer (cat no. T10796; Invitrogen).

    Mouse Assay:

    Article Title: Heat shock protein‐27 and sex‐selective regulation of muscarinic and proteinase‐activated receptor 2‐mediated vasodilatation: differential sensitivity to endothelial NOS inhibition
    Article Snippet: After removal of endotoxin with Detoxi‐gel columns (Fisher Scientific, Pittsburgh, PA, USA), the purity of the final rHSP27 and rC1 protein was more than 95% by SDS‐PAGE and the endotoxin concentration was < 5 EU·mg−1 protein (LAL assay). .. Aorta tissues harvested from female HSPB1‐null mice were cultured for either 3 or 24 h at 37°C in serum‐free DMEM containing 10 mM glucose (a euglycaemic value for mice), GlutaMAX™ supplement, with pyruvate (Life technologies, Canada, CAT #10567014), either in the absence or presence of 50 μg·mL−1 (2.2 μM) recombinant rHSP27 prepared as previously described (Salari et al., ). ..

    Cell Culture:

    Article Title: Heat shock protein‐27 and sex‐selective regulation of muscarinic and proteinase‐activated receptor 2‐mediated vasodilatation: differential sensitivity to endothelial NOS inhibition
    Article Snippet: After removal of endotoxin with Detoxi‐gel columns (Fisher Scientific, Pittsburgh, PA, USA), the purity of the final rHSP27 and rC1 protein was more than 95% by SDS‐PAGE and the endotoxin concentration was < 5 EU·mg−1 protein (LAL assay). .. Aorta tissues harvested from female HSPB1‐null mice were cultured for either 3 or 24 h at 37°C in serum‐free DMEM containing 10 mM glucose (a euglycaemic value for mice), GlutaMAX™ supplement, with pyruvate (Life technologies, Canada, CAT #10567014), either in the absence or presence of 50 μg·mL−1 (2.2 μM) recombinant rHSP27 prepared as previously described (Salari et al., ). ..

    Article Title: Senescence-associated secretory factors induced by cisplatin in melanoma cells promote non-senescent melanoma cell growth through activation of the ERK1/2-RSK1 pathway
    Article Snippet: At different intervals, alamarBlue was added and fluorescence intensity was detected using a microplate reader. .. A375 cells were seeded in triplicate and serum starved for 48 h. Then, DMEM medium with 0.25% FBS (negative control, NC), DMEM+ NS CM (1:1) and DMEM+ Sen CM (1:1) was added into one of the three wells, respectively, and cultured for 9 h. The cells were collected and mRNA array was performed using Affymetrix PrimeView Human Gene Expression Array (USA). ..

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Heat shock protein‐27 and sex‐selective regulation of muscarinic and proteinase‐activated receptor 2‐mediated vasodilatation: differential sensitivity to endothelial NOS inhibition
    Article Snippet: After removal of endotoxin with Detoxi‐gel columns (Fisher Scientific, Pittsburgh, PA, USA), the purity of the final rHSP27 and rC1 protein was more than 95% by SDS‐PAGE and the endotoxin concentration was < 5 EU·mg−1 protein (LAL assay). .. Aorta tissues harvested from female HSPB1‐null mice were cultured for either 3 or 24 h at 37°C in serum‐free DMEM containing 10 mM glucose (a euglycaemic value for mice), GlutaMAX™ supplement, with pyruvate (Life technologies, Canada, CAT #10567014), either in the absence or presence of 50 μg·mL−1 (2.2 μM) recombinant rHSP27 prepared as previously described (Salari et al., ). ..

    Recombinant:

    Article Title: Heat shock protein‐27 and sex‐selective regulation of muscarinic and proteinase‐activated receptor 2‐mediated vasodilatation: differential sensitivity to endothelial NOS inhibition
    Article Snippet: After removal of endotoxin with Detoxi‐gel columns (Fisher Scientific, Pittsburgh, PA, USA), the purity of the final rHSP27 and rC1 protein was more than 95% by SDS‐PAGE and the endotoxin concentration was < 5 EU·mg−1 protein (LAL assay). .. Aorta tissues harvested from female HSPB1‐null mice were cultured for either 3 or 24 h at 37°C in serum‐free DMEM containing 10 mM glucose (a euglycaemic value for mice), GlutaMAX™ supplement, with pyruvate (Life technologies, Canada, CAT #10567014), either in the absence or presence of 50 μg·mL−1 (2.2 μM) recombinant rHSP27 prepared as previously described (Salari et al., ). ..

    Migration:

    Article Title: Plexin-B2 Negatively Regulates Macrophage Motility, Rac, and Cdc42 Activation
    Article Snippet: .. Migration Macrophages were M-CSF starved in complete DMEM overnight, harvested, and seeded in plain DMEM at 2×105 per upper well of 96-well transwell plates with 8 µm pores (ChemoTx System; NeuroProbe, Gaithersburg, MD), over chemokines (Peprotech, Rocky Hill, NJ) in serum-free DMEM or FBS (Gibco, Carlsbad, California) in DMEM, and incubated at 37°C for 4 hours for migration towards chemokine or overnight for migration towards serum. .. Migrated cells were quantified using ToxiLight Bioassay Kit (Lonza, Rockland, ME).

    Incubation:

    Article Title: Plexin-B2 Negatively Regulates Macrophage Motility, Rac, and Cdc42 Activation
    Article Snippet: .. Migration Macrophages were M-CSF starved in complete DMEM overnight, harvested, and seeded in plain DMEM at 2×105 per upper well of 96-well transwell plates with 8 µm pores (ChemoTx System; NeuroProbe, Gaithersburg, MD), over chemokines (Peprotech, Rocky Hill, NJ) in serum-free DMEM or FBS (Gibco, Carlsbad, California) in DMEM, and incubated at 37°C for 4 hours for migration towards chemokine or overnight for migration towards serum. .. Migrated cells were quantified using ToxiLight Bioassay Kit (Lonza, Rockland, ME).

    Transfection:

    Article Title: Small-Molecule “BRCA1-Mimetics” Are Antagonists of Estrogen Receptor-α
    Article Snippet: Western blotting experiments showed that a minimum of 48 hours of exposure to 50 nM of BRCA1 -siRNA was required to obtain a large reduction ( > 75%) of the BRCA1 protein levels. .. Subconfluent cells in 24-well dishes were transfected overnight with 0.25 μg of each indicated vector plus the ERE-TK-Luc reporter in serum-free DMEM containing Lipofectamine 2000 (Life Technologies). ..

    Plasmid Preparation:

    Article Title: Small-Molecule “BRCA1-Mimetics” Are Antagonists of Estrogen Receptor-α
    Article Snippet: Western blotting experiments showed that a minimum of 48 hours of exposure to 50 nM of BRCA1 -siRNA was required to obtain a large reduction ( > 75%) of the BRCA1 protein levels. .. Subconfluent cells in 24-well dishes were transfected overnight with 0.25 μg of each indicated vector plus the ERE-TK-Luc reporter in serum-free DMEM containing Lipofectamine 2000 (Life Technologies). ..

    Negative Control:

    Article Title: Senescence-associated secretory factors induced by cisplatin in melanoma cells promote non-senescent melanoma cell growth through activation of the ERK1/2-RSK1 pathway
    Article Snippet: At different intervals, alamarBlue was added and fluorescence intensity was detected using a microplate reader. .. A375 cells were seeded in triplicate and serum starved for 48 h. Then, DMEM medium with 0.25% FBS (negative control, NC), DMEM+ NS CM (1:1) and DMEM+ Sen CM (1:1) was added into one of the three wells, respectively, and cultured for 9 h. The cells were collected and mRNA array was performed using Affymetrix PrimeView Human Gene Expression Array (USA). ..

    Expressing:

    Article Title: Senescence-associated secretory factors induced by cisplatin in melanoma cells promote non-senescent melanoma cell growth through activation of the ERK1/2-RSK1 pathway
    Article Snippet: At different intervals, alamarBlue was added and fluorescence intensity was detected using a microplate reader. .. A375 cells were seeded in triplicate and serum starved for 48 h. Then, DMEM medium with 0.25% FBS (negative control, NC), DMEM+ NS CM (1:1) and DMEM+ Sen CM (1:1) was added into one of the three wells, respectively, and cultured for 9 h. The cells were collected and mRNA array was performed using Affymetrix PrimeView Human Gene Expression Array (USA). ..

    Derivative Assay:

    Article Title: Developing a New Two-Step Protocol to Generate Functional Hepatocytes from Wharton's Jelly-Derived Mesenchymal Stem Cells under Hypoxic Condition
    Article Snippet: .. WJMSCs-SUT1 and WJMSCs-SUT2 were derived from the cultivation of WJ-MSCs in Dulbecco's modified Eagle's medium with 1.0 g/L glucose (DMEM-LG) (Invitrogen, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (HyClone, Logan, UT, USA) and embryonic stem cells conditioned medium (ESCM) at 37°C with 5% CO2 and 5% O2 , respectively. ..

    Modification:

    Article Title: Developing a New Two-Step Protocol to Generate Functional Hepatocytes from Wharton's Jelly-Derived Mesenchymal Stem Cells under Hypoxic Condition
    Article Snippet: .. WJMSCs-SUT1 and WJMSCs-SUT2 were derived from the cultivation of WJ-MSCs in Dulbecco's modified Eagle's medium with 1.0 g/L glucose (DMEM-LG) (Invitrogen, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (HyClone, Logan, UT, USA) and embryonic stem cells conditioned medium (ESCM) at 37°C with 5% CO2 and 5% O2 , respectively. ..

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    Thermo Fisher dmem ko
    Tri-lineage differentiation. A-I) Representative images of XF,SFM-cultured and <t>DMEM</t> KO + 10% <t>FBS-cultured</t> MSCs, differentiated into adipocytes, osteocytes and chondrocytes, as visualized by Oil Red O, von Kossa and alcian blue stains, respectively, against undifferentiated controls; J) Bar graph to quantify oil droplets, post Oil Red O staining; K) Bar graph to quantify mineralization, post alizarin red staining; L) Bar graph to quantify sulfated glycosaminoglycan production by differentiated chondrocytes, normalized to DNA content. FBS, fetal bovine serum; MSCs, mesenchymal stem cells.
    Dmem Ko, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dmem ko/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dmem ko - by Bioz Stars, 2021-03
    97/100 stars
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    99
    Thermo Fisher knockout dulbecco s modified eagle medium ko dmem
    Hepatic differentiation of iPSCs through the definitive endoderm (DE) stage. (A) A schematic representation of the hepatic differentiation protocol (modified from Hay et al., 2008 ) and cell culture media used at each stage. See text for details of the media. (B) Phase-contrast images showing sequential morphological changes from iPSCs (day 0) to DE (day 5) and finally hepatocyte-like cells (HLCs) (day 20) during differentiation. (C,D) Immunocytochemistry of the iPSC-HLCs differentiated from three patient lines (UTA.10100.EURCAs, UTA.11104.EURCAs and UTA.11304.EURCCs) at day 20 showing the expression of (C) LDL receptor (LDL-R) and asialoglycoprotein receptor (ASGR), as well as (D) α-fetoprotein (AFP) and albumin (ALB). Nuclei are stained with DAPI. (E) The iPSC-HLCs were able to uptake LDL at day 20 of differentiation and (F) produce and secrete albumin during differentiation. Values are normalised per 1 million cells per 24 h. Bars represent means±s.d. of three biological replicates. Gene expression levels of (G) apolipoprotein B ( ApoB ) and (H) apolipoprotein AI ( ApoAI ) at different time points during the iPSC to HLC differentiation normalised to the housekeeping gene GAPDH , and expressed relative to day 0. Each sample was run in triplicate and bars represent means±s.d. of three studied cell lines. iPSC, induced pluripotent stem cell; <t>KO-DMEM,</t> KnockOut <t>Dulbecco's</t> modified Eagle medium; DMSO, dimethyl sulfoxide; HGF, hepatocyte growth factor; OSM, oncostatin M; PHH, primary human hepatocyte. Scale bars: 200 μm.
    Knockout Dulbecco S Modified Eagle Medium Ko Dmem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/knockout dulbecco s modified eagle medium ko dmem/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    knockout dulbecco s modified eagle medium ko dmem - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    Tri-lineage differentiation. A-I) Representative images of XF,SFM-cultured and DMEM KO + 10% FBS-cultured MSCs, differentiated into adipocytes, osteocytes and chondrocytes, as visualized by Oil Red O, von Kossa and alcian blue stains, respectively, against undifferentiated controls; J) Bar graph to quantify oil droplets, post Oil Red O staining; K) Bar graph to quantify mineralization, post alizarin red staining; L) Bar graph to quantify sulfated glycosaminoglycan production by differentiated chondrocytes, normalized to DNA content. FBS, fetal bovine serum; MSCs, mesenchymal stem cells.

    Journal: Stem Cell Research & Therapy

    Article Title: Are serum-free and xeno-free culture conditions ideal for large scale clinical grade expansion of Wharton’s jelly derived mesenchymal stem cells? A comparative study

    doi: 10.1186/scrt477

    Figure Lengend Snippet: Tri-lineage differentiation. A-I) Representative images of XF,SFM-cultured and DMEM KO + 10% FBS-cultured MSCs, differentiated into adipocytes, osteocytes and chondrocytes, as visualized by Oil Red O, von Kossa and alcian blue stains, respectively, against undifferentiated controls; J) Bar graph to quantify oil droplets, post Oil Red O staining; K) Bar graph to quantify mineralization, post alizarin red staining; L) Bar graph to quantify sulfated glycosaminoglycan production by differentiated chondrocytes, normalized to DNA content. FBS, fetal bovine serum; MSCs, mesenchymal stem cells.

    Article Snippet: Annexin V staining We looked for the presence of apoptotic cells among cultures in DMEM KO + 10% FBS and MesenCult, using the Tali Apoptosis Kit – Annexin V Alexa Flour 488 and propidium iodide (PI; cat no. 10788; Invitrogen), according to the manufacturer’s instructions.

    Techniques: Cell Culture, Staining

    Growth kinetics and CFU-F efficacy. A-F) Representative morphology pictures of WJ derived MSCs cultured in DMEM KO + 10% FBS and MesenCult XF,SF media; G,H,I) Total cell yield obtained at passage 5 using XF,SF medium, compared against DMEM KO + 10% FBS; K) Average number of colonies generated per 100 cells at passage 3 and passage 4, by cells cultured in XF,SF media and DMEM KO + 10% FBS; J,L) Individual colonies captured at 4X magnification, for XF,SFM cultures and DMEM KO + 10% FBS cultures, respectively. FBS, fetal bovine serum; GFU-F, colony-forming unit-fibroblast; MSCs, mesenchymal stem cells; WJ, Wharton’s jelly.

    Journal: Stem Cell Research & Therapy

    Article Title: Are serum-free and xeno-free culture conditions ideal for large scale clinical grade expansion of Wharton’s jelly derived mesenchymal stem cells? A comparative study

    doi: 10.1186/scrt477

    Figure Lengend Snippet: Growth kinetics and CFU-F efficacy. A-F) Representative morphology pictures of WJ derived MSCs cultured in DMEM KO + 10% FBS and MesenCult XF,SF media; G,H,I) Total cell yield obtained at passage 5 using XF,SF medium, compared against DMEM KO + 10% FBS; K) Average number of colonies generated per 100 cells at passage 3 and passage 4, by cells cultured in XF,SF media and DMEM KO + 10% FBS; J,L) Individual colonies captured at 4X magnification, for XF,SFM cultures and DMEM KO + 10% FBS cultures, respectively. FBS, fetal bovine serum; GFU-F, colony-forming unit-fibroblast; MSCs, mesenchymal stem cells; WJ, Wharton’s jelly.

    Article Snippet: Annexin V staining We looked for the presence of apoptotic cells among cultures in DMEM KO + 10% FBS and MesenCult, using the Tali Apoptosis Kit – Annexin V Alexa Flour 488 and propidium iodide (PI; cat no. 10788; Invitrogen), according to the manufacturer’s instructions.

    Techniques: Derivative Assay, Cell Culture, Generated

    Senescence, apoptosis and transformation. A) Percentage of senescent cells in XF,SFM cultures, compared against DMEM KO + 10% FBS cultures at P5 followed by representative 10 X images of fields showing blue colored senescent cells; B) Percentage of Annexin positive (apoptotic) cells in XF,SFM cultures compared to DMEM KO + 10% FBS cultures at P5 followed by representative images of fields showing green colored apoptotic cells; C) Representative pictures of cells cultured in soft agar medium, showing no colonies in XF,SFM and DMEM KO + 10% FBS cultures, compared to colonies observed in MCF-7 cultures; D) Comparative gene expression analysis between cells cultured in XF,SF medium and DMEM KO + 10% FBS at P6. FBS, fetal bovine serum; P, passage.

    Journal: Stem Cell Research & Therapy

    Article Title: Are serum-free and xeno-free culture conditions ideal for large scale clinical grade expansion of Wharton’s jelly derived mesenchymal stem cells? A comparative study

    doi: 10.1186/scrt477

    Figure Lengend Snippet: Senescence, apoptosis and transformation. A) Percentage of senescent cells in XF,SFM cultures, compared against DMEM KO + 10% FBS cultures at P5 followed by representative 10 X images of fields showing blue colored senescent cells; B) Percentage of Annexin positive (apoptotic) cells in XF,SFM cultures compared to DMEM KO + 10% FBS cultures at P5 followed by representative images of fields showing green colored apoptotic cells; C) Representative pictures of cells cultured in soft agar medium, showing no colonies in XF,SFM and DMEM KO + 10% FBS cultures, compared to colonies observed in MCF-7 cultures; D) Comparative gene expression analysis between cells cultured in XF,SF medium and DMEM KO + 10% FBS at P6. FBS, fetal bovine serum; P, passage.

    Article Snippet: Annexin V staining We looked for the presence of apoptotic cells among cultures in DMEM KO + 10% FBS and MesenCult, using the Tali Apoptosis Kit – Annexin V Alexa Flour 488 and propidium iodide (PI; cat no. 10788; Invitrogen), according to the manufacturer’s instructions.

    Techniques: Transformation Assay, Cell Culture, Expressing

    Immunomodulation, karyotype and cell cycle analysis. A) Bar graph showing percentage suppression of PBMC-generated MLRs, by cells cultured in XF,SF medium, against those cultured in DMEM KO + 10% FBS, at passages 4 and 6; B) Percentage proliferation brought about by co-culturing MSCs (cultured in both conditions) with human peripheral blood-derived PBMCs, at passages 4 and 6, shown against a one-way mixed lymphocyte reaction taken as 100% proliferation; C and D) Representative karyotypes of cells cultured in XF,SF medium and DMEM KO + 10% FBS, respectively, at passage 5; E) Percentage of cells cultured in XF,SFM and DMEM KO + 10% FBS, in S phase at passage 5; F) Percentage of cells cultured in XF,SF medium and DMEM KO + 10% FBS in G0/G1, S and M phases, at passage 5. FBS, fetal bovine serum; PBMC, peripheral blood mononuclear cells.

    Journal: Stem Cell Research & Therapy

    Article Title: Are serum-free and xeno-free culture conditions ideal for large scale clinical grade expansion of Wharton’s jelly derived mesenchymal stem cells? A comparative study

    doi: 10.1186/scrt477

    Figure Lengend Snippet: Immunomodulation, karyotype and cell cycle analysis. A) Bar graph showing percentage suppression of PBMC-generated MLRs, by cells cultured in XF,SF medium, against those cultured in DMEM KO + 10% FBS, at passages 4 and 6; B) Percentage proliferation brought about by co-culturing MSCs (cultured in both conditions) with human peripheral blood-derived PBMCs, at passages 4 and 6, shown against a one-way mixed lymphocyte reaction taken as 100% proliferation; C and D) Representative karyotypes of cells cultured in XF,SF medium and DMEM KO + 10% FBS, respectively, at passage 5; E) Percentage of cells cultured in XF,SFM and DMEM KO + 10% FBS, in S phase at passage 5; F) Percentage of cells cultured in XF,SF medium and DMEM KO + 10% FBS in G0/G1, S and M phases, at passage 5. FBS, fetal bovine serum; PBMC, peripheral blood mononuclear cells.

    Article Snippet: Annexin V staining We looked for the presence of apoptotic cells among cultures in DMEM KO + 10% FBS and MesenCult, using the Tali Apoptosis Kit – Annexin V Alexa Flour 488 and propidium iodide (PI; cat no. 10788; Invitrogen), according to the manufacturer’s instructions.

    Techniques: Cell Cycle Assay, Generated, Cell Culture, Derivative Assay

    Angiogenic potency. A, B) Real-time PCR quantification of HGF, VEGF, TGFβ-1 and IL6 for cells cultured in DMEM KO + 10% FBS and in XF,SFM, all normalized against Cord 1 (DMEM KO + 10% FBS); C,D,E) Box graphs representing variations observed between cells cultured in XF,SFM and FBS-containing medium, when analyzed for cytokine secretions of TGFβ, HGF and Ang1; F) Tube formation efficiency (estimated using HUVEC, cultured on growth factor reduced Matrigel) of WJ-MSC-conditioned medium, from three cords cultured in DMEM KO + 10% FBS and in XF, SFM. Anti-HGF neutralizing antibody was used to block tube formation in each condition. G-J’) Representative 4 X pictures showing tubes formed. FBS, fetal bovine serum; HGF, hepatocyte growth factor; TGFβ1, transforming growth factor-β1; VEGF, vascular endothelial growth factor. ** signifies extent of significance of the numeric value.

    Journal: Stem Cell Research & Therapy

    Article Title: Are serum-free and xeno-free culture conditions ideal for large scale clinical grade expansion of Wharton’s jelly derived mesenchymal stem cells? A comparative study

    doi: 10.1186/scrt477

    Figure Lengend Snippet: Angiogenic potency. A, B) Real-time PCR quantification of HGF, VEGF, TGFβ-1 and IL6 for cells cultured in DMEM KO + 10% FBS and in XF,SFM, all normalized against Cord 1 (DMEM KO + 10% FBS); C,D,E) Box graphs representing variations observed between cells cultured in XF,SFM and FBS-containing medium, when analyzed for cytokine secretions of TGFβ, HGF and Ang1; F) Tube formation efficiency (estimated using HUVEC, cultured on growth factor reduced Matrigel) of WJ-MSC-conditioned medium, from three cords cultured in DMEM KO + 10% FBS and in XF, SFM. Anti-HGF neutralizing antibody was used to block tube formation in each condition. G-J’) Representative 4 X pictures showing tubes formed. FBS, fetal bovine serum; HGF, hepatocyte growth factor; TGFβ1, transforming growth factor-β1; VEGF, vascular endothelial growth factor. ** signifies extent of significance of the numeric value.

    Article Snippet: Annexin V staining We looked for the presence of apoptotic cells among cultures in DMEM KO + 10% FBS and MesenCult, using the Tali Apoptosis Kit – Annexin V Alexa Flour 488 and propidium iodide (PI; cat no. 10788; Invitrogen), according to the manufacturer’s instructions.

    Techniques: Real-time Polymerase Chain Reaction, Cell Culture, Blocking Assay

    Flowcytometric characterization of MSCs and CAM of omentum fat derived MSC. Characterization of omentum fat derived MSC at early (P3) and late (P20) passages in DMEM-LG, Alpha-MEM, DMEM-F12, and DMEM-KO for CD90, CD105, CD73, CD29, CD49d, CD44, CD166, CD106, CD54, and CD31 using flowcytometry.

    Journal: Stem Cells International

    Article Title: Human Adult Stem Cells Maintain a Constant Phenotype Profile Irrespective of Their Origin, Basal Media, and Long Term Cultures

    doi: 10.1155/2015/146051

    Figure Lengend Snippet: Flowcytometric characterization of MSCs and CAM of omentum fat derived MSC. Characterization of omentum fat derived MSC at early (P3) and late (P20) passages in DMEM-LG, Alpha-MEM, DMEM-F12, and DMEM-KO for CD90, CD105, CD73, CD29, CD49d, CD44, CD166, CD106, CD54, and CD31 using flowcytometry.

    Article Snippet: Cell Culture Cells isolated from these aforesaid sources were plated at a density of 3 × 105 /25 cm2 flask (Nunc) and cultured in four different filter sterilized media: DMEM-LG (Invitrogen), α -MEM (Invitrogen), DMEM-F12 (Invitrogen), and DMEM-KO (Invitrogen), each of which was supplemented with 10% FBS (Invitrogen) and 1% antibiotic-antimycotic solution.

    Techniques: Chick Chorioallantoic Membrane Assay, Derivative Assay

    Flowcytometric characterization of HSC and unique markers of omentum fat derived MSC. Characterization of omentum fat derived MSC at early (P3) and late (P20) passages in DMEM-LG, Alpha-MEM, DMEM-F12, and DMEM-KO for CD34, CD45, CD133, HLA-DR, CD117, ABCG2, ALDH, SSEA4, and CD13 using flowcytometry.

    Journal: Stem Cells International

    Article Title: Human Adult Stem Cells Maintain a Constant Phenotype Profile Irrespective of Their Origin, Basal Media, and Long Term Cultures

    doi: 10.1155/2015/146051

    Figure Lengend Snippet: Flowcytometric characterization of HSC and unique markers of omentum fat derived MSC. Characterization of omentum fat derived MSC at early (P3) and late (P20) passages in DMEM-LG, Alpha-MEM, DMEM-F12, and DMEM-KO for CD34, CD45, CD133, HLA-DR, CD117, ABCG2, ALDH, SSEA4, and CD13 using flowcytometry.

    Article Snippet: Cell Culture Cells isolated from these aforesaid sources were plated at a density of 3 × 105 /25 cm2 flask (Nunc) and cultured in four different filter sterilized media: DMEM-LG (Invitrogen), α -MEM (Invitrogen), DMEM-F12 (Invitrogen), and DMEM-KO (Invitrogen), each of which was supplemented with 10% FBS (Invitrogen) and 1% antibiotic-antimycotic solution.

    Techniques: Derivative Assay

    Marker expression profiling of MSCs cultured in DMEM-F12. Comparative Surface antigenic profiling of MSCs derived from omentum fat, subcutaneous fat, and bone marrow at early (P3) and late (P20) passages cultured in DMEM-F12.

    Journal: Stem Cells International

    Article Title: Human Adult Stem Cells Maintain a Constant Phenotype Profile Irrespective of Their Origin, Basal Media, and Long Term Cultures

    doi: 10.1155/2015/146051

    Figure Lengend Snippet: Marker expression profiling of MSCs cultured in DMEM-F12. Comparative Surface antigenic profiling of MSCs derived from omentum fat, subcutaneous fat, and bone marrow at early (P3) and late (P20) passages cultured in DMEM-F12.

    Article Snippet: Cell Culture Cells isolated from these aforesaid sources were plated at a density of 3 × 105 /25 cm2 flask (Nunc) and cultured in four different filter sterilized media: DMEM-LG (Invitrogen), α -MEM (Invitrogen), DMEM-F12 (Invitrogen), and DMEM-KO (Invitrogen), each of which was supplemented with 10% FBS (Invitrogen) and 1% antibiotic-antimycotic solution.

    Techniques: Marker, Expressing, Cell Culture, Derivative Assay

    Adipogenic differentiation of bone marrow derived MSC. Confirmation of adipogenesis using Oil Red O staining at early - P3 ( a – e ) and late passages - P20 ( f – j ) control: a , f ; DMEM-LG: b , g ; ALPHA-MEM: c , h ; DMEM-F12: d , i ; DMEM-KO: e , j )

    Journal: Cytotechnology

    Article Title: A comprehensive study on optimization of proliferation and differentiation potency of bone marrow derived mesenchymal stem cells under prolonged culture condition

    doi: 10.1007/s10616-012-9471-0

    Figure Lengend Snippet: Adipogenic differentiation of bone marrow derived MSC. Confirmation of adipogenesis using Oil Red O staining at early - P3 ( a – e ) and late passages - P20 ( f – j ) control: a , f ; DMEM-LG: b , g ; ALPHA-MEM: c , h ; DMEM-F12: d , i ; DMEM-KO: e , j )

    Article Snippet: Cells were plated at a density of 3.4 × 104 /cm2 in T-25 flasks (Nunc, Roskilde, Denmark) and cultured in four different media: DMEM-LG (Invitrogen, Bangalore, India), α-MEM (Invitrogen), DMEM-F12 (Invitrogen), and in DMEM–KO (Invitrogen), each was supplemented with 10 % FBS (Invitrogen) and 1 % antibiotic–antimycotic solution (Invitrogen).

    Techniques: Derivative Assay, Staining

    Population doubling time analysis of Bone marrow derived MSC. Population doubling time analysis of bone marrow derived MSC at early-P3 and late -P20 passage with respect to different media: DMEM-LG, α-MEM, DMEM-F12 and DMEM–KO

    Journal: Cytotechnology

    Article Title: A comprehensive study on optimization of proliferation and differentiation potency of bone marrow derived mesenchymal stem cells under prolonged culture condition

    doi: 10.1007/s10616-012-9471-0

    Figure Lengend Snippet: Population doubling time analysis of Bone marrow derived MSC. Population doubling time analysis of bone marrow derived MSC at early-P3 and late -P20 passage with respect to different media: DMEM-LG, α-MEM, DMEM-F12 and DMEM–KO

    Article Snippet: Cells were plated at a density of 3.4 × 104 /cm2 in T-25 flasks (Nunc, Roskilde, Denmark) and cultured in four different media: DMEM-LG (Invitrogen, Bangalore, India), α-MEM (Invitrogen), DMEM-F12 (Invitrogen), and in DMEM–KO (Invitrogen), each was supplemented with 10 % FBS (Invitrogen) and 1 % antibiotic–antimycotic solution (Invitrogen).

    Techniques: Derivative Assay

    Osteogenic differentiation of bone marrow derived MSC. Confirmation of osteogenesis using von Kossa staining at early - P3 ( a – e ) and late passages-P20 ( f – j ) control: a , f ; DMEM-LG: b , g ; ALPHA-MEM: c , h ; DMEM-F12: d , i ; DMEM-KO: e , j ) Alizarin

    Journal: Cytotechnology

    Article Title: A comprehensive study on optimization of proliferation and differentiation potency of bone marrow derived mesenchymal stem cells under prolonged culture condition

    doi: 10.1007/s10616-012-9471-0

    Figure Lengend Snippet: Osteogenic differentiation of bone marrow derived MSC. Confirmation of osteogenesis using von Kossa staining at early - P3 ( a – e ) and late passages-P20 ( f – j ) control: a , f ; DMEM-LG: b , g ; ALPHA-MEM: c , h ; DMEM-F12: d , i ; DMEM-KO: e , j ) Alizarin

    Article Snippet: Cells were plated at a density of 3.4 × 104 /cm2 in T-25 flasks (Nunc, Roskilde, Denmark) and cultured in four different media: DMEM-LG (Invitrogen, Bangalore, India), α-MEM (Invitrogen), DMEM-F12 (Invitrogen), and in DMEM–KO (Invitrogen), each was supplemented with 10 % FBS (Invitrogen) and 1 % antibiotic–antimycotic solution (Invitrogen).

    Techniques: Derivative Assay, Staining

    Hepatic differentiation of iPSCs through the definitive endoderm (DE) stage. (A) A schematic representation of the hepatic differentiation protocol (modified from Hay et al., 2008 ) and cell culture media used at each stage. See text for details of the media. (B) Phase-contrast images showing sequential morphological changes from iPSCs (day 0) to DE (day 5) and finally hepatocyte-like cells (HLCs) (day 20) during differentiation. (C,D) Immunocytochemistry of the iPSC-HLCs differentiated from three patient lines (UTA.10100.EURCAs, UTA.11104.EURCAs and UTA.11304.EURCCs) at day 20 showing the expression of (C) LDL receptor (LDL-R) and asialoglycoprotein receptor (ASGR), as well as (D) α-fetoprotein (AFP) and albumin (ALB). Nuclei are stained with DAPI. (E) The iPSC-HLCs were able to uptake LDL at day 20 of differentiation and (F) produce and secrete albumin during differentiation. Values are normalised per 1 million cells per 24 h. Bars represent means±s.d. of three biological replicates. Gene expression levels of (G) apolipoprotein B ( ApoB ) and (H) apolipoprotein AI ( ApoAI ) at different time points during the iPSC to HLC differentiation normalised to the housekeeping gene GAPDH , and expressed relative to day 0. Each sample was run in triplicate and bars represent means±s.d. of three studied cell lines. iPSC, induced pluripotent stem cell; KO-DMEM, KnockOut Dulbecco's modified Eagle medium; DMSO, dimethyl sulfoxide; HGF, hepatocyte growth factor; OSM, oncostatin M; PHH, primary human hepatocyte. Scale bars: 200 μm.

    Journal: Disease Models & Mechanisms

    Article Title: Lipidomic profiling of patient-specific iPSC-derived hepatocyte-like cells

    doi: 10.1242/dmm.030841

    Figure Lengend Snippet: Hepatic differentiation of iPSCs through the definitive endoderm (DE) stage. (A) A schematic representation of the hepatic differentiation protocol (modified from Hay et al., 2008 ) and cell culture media used at each stage. See text for details of the media. (B) Phase-contrast images showing sequential morphological changes from iPSCs (day 0) to DE (day 5) and finally hepatocyte-like cells (HLCs) (day 20) during differentiation. (C,D) Immunocytochemistry of the iPSC-HLCs differentiated from three patient lines (UTA.10100.EURCAs, UTA.11104.EURCAs and UTA.11304.EURCCs) at day 20 showing the expression of (C) LDL receptor (LDL-R) and asialoglycoprotein receptor (ASGR), as well as (D) α-fetoprotein (AFP) and albumin (ALB). Nuclei are stained with DAPI. (E) The iPSC-HLCs were able to uptake LDL at day 20 of differentiation and (F) produce and secrete albumin during differentiation. Values are normalised per 1 million cells per 24 h. Bars represent means±s.d. of three biological replicates. Gene expression levels of (G) apolipoprotein B ( ApoB ) and (H) apolipoprotein AI ( ApoAI ) at different time points during the iPSC to HLC differentiation normalised to the housekeeping gene GAPDH , and expressed relative to day 0. Each sample was run in triplicate and bars represent means±s.d. of three studied cell lines. iPSC, induced pluripotent stem cell; KO-DMEM, KnockOut Dulbecco's modified Eagle medium; DMSO, dimethyl sulfoxide; HGF, hepatocyte growth factor; OSM, oncostatin M; PHH, primary human hepatocyte. Scale bars: 200 μm.

    Article Snippet: Cells were treated with KnockOut Dulbecco's modified Eagle medium (KO-DMEM) supplemented with 20% KnockOut Serum Replacement (Ko-SR), 2 mM GlutaMAX, 0.1 mM 2-mercaptoethanol (2-ME) (all from Gibco), 1% nonessential amino acids (NEAA) and 50 U/ml penicillin/streptomycin (both from LONZA).

    Techniques: Modification, Cell Culture, Immunocytochemistry, Expressing, Staining, Knock-Out