dmem high glucose (GE Healthcare)
95
|
Name:
DMEM High glucose without L glutamine and sodium pyruvate
Description:
Catalog Number:
sh30081.01
Price:
21.81 USD
Size:
500 mL
|
Buy from Supplier |
Structured Review
GE Healthcare
dmem high glucose
https://www.bioz.com/result/dmem high glucose/product/GE Healthcare
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
https://www.bioz.com/result/dmem high glucose/product/GE Healthcare
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
dmem high glucose - by Bioz Stars,
2021-03
95/100 stars
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![MGF stimulates glucose oxidation. Palmitate ( A ) and glucose ( B ) oxidation was measured after a 12-h fast in soleus muscle isolated from mice fed with CD or HFD, with or without 0.5% MGF, for 18 weeks ( n = 6–7). C : [ 14 C]-labeled oleic acid oxidation in C2C12 myotubes pretreated or not with 200 μmol/L MGF for 16 h. Palmitate ( D ) and glucose ( E ) oxidation in C2C12 myotubes measured by Seahorse Bioscience Metabolic Flux analyzer. Myotubes differentiated from C2C12 cells in XF24 microplates were incubated overnight in glucose-free and sodium pyruvate–free DMEM, with or without 200 μmol/L MGF. OCR was recorded after injection of vehicle ( D ), BSA-conjugated palmitate ( D ), or d -glucose ( E ) into wells, with or without 200 μmol/L MGF. F : Intracellular ATP levels in C2C12 myotubes incubated overnight in glucose-free and sodium pyruvate–free DMEM in the presence of various concentrations of MGF and then incubated in media containing 25 mmol/L glucose with matching concentrations of MGF for 8 h. ATP was determined using the CellTiter-Glo Luminescent Cell Viability Assay kit according to the manufacturer’s instructions (Promega). In vitro experiments were conducted three to four times and each time with triplicates for each condition. Values are average ± SEM. * P](https://storage.googleapis.com/bioz_article_images/PMC4207399/3626fig4.jpg)
![MGF enhances pyruvate oxidation by activating PDH. A : Pyruvate oxidation in myotubes differentiated from C2C12 cells measured by Seahorse Bioscience Metabolic Flux analysis. Myotubes differentiated from C2C12 cells in XF24 microplates were incubated overnight in glucose-free and sodium pyruvate–free DMEM, with and without 200 μmol/L MGF. OCR was recorded after injection of sodium pyruvate into wells, with or without 200 μmol/L MGF. B : PDH activities measured as the difference between absorbances at 500 nm and 750 nm at 25°C in mitochondrial protein from C2C12 myotubes treated with various concentrations of MGF for 24 h or 15 min or with 5 mmol/L DCA or FP + 200 μmol/L MGF for 15 min. C : Initial rates of PDH-catalyzed pyruvate oxidation measured as the difference between absorbances at 500 nm and 750 nm in mitochondrial protein from C2C12 myotubes treated with various concentrations of MGF for 24 h or 15 min. The unit of activity was determined by using extinction coefficient of reduced p-iodonitrotetrazolium violet (12.4 mmol ⋅ L −1 ⋅ cm −1 ). D : Initial rates of PDH catalyzed [ 14 C]-labeled pyruvate oxidation measured as the production of 14 CO 2 by mitochondrial protein from C2C12 myotubes treated with various concentrations of MGF for 15 min. E : Western blots of proteins extracted from C2C12 myotubes treated with 200 or 1,000 μmol/L MGF for 15 min and 24 h. Membrane was blotted with PDH (1:250 dilution; Cell Signaling), p-PDH (1:2,000; Abcam), PDK4 (1:500; laboratory of Dr. Robert Harris at Indiana University School of Medicine), or α-tubulin (1:2,000; Sigma-Aldrich) at 4°C overnight. F : Quantitative analysis of Western blots shown in E . G : Western blots of proteins extracted from C2C12 myotubes treated with 200 or 1,000 μmol/L MGF or 5 mmol/L DCA for 15 min. H : Quantitative analysis of Western blots shown in G . Values are average ± SEM. * P](https://storage.googleapis.com/bioz_article_images/PMC4207399/3626fig6.jpg)
