dmem high glucose  (GE Healthcare)

 
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    Name:
    DMEM High glucose without L glutamine and sodium pyruvate
    Description:

    Catalog Number:
    sh30081.01
    Price:
    21.81 USD
    Size:
    500 mL
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    Structured Review

    GE Healthcare dmem high glucose

    https://www.bioz.com/result/dmem high glucose/product/GE Healthcare
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dmem high glucose - by Bioz Stars, 2021-03
    95/100 stars

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    Cell Culture:

    Article Title: PEX2 is the E3 ubiquitin ligase required for pexophagy during starvation
    Article Snippet: Fluorescent goat polyclonal α-rabbit Alexa Fluor 568 secondary antibody and goat polyclonal α-mouse Alexa Fluor 488 secondary antibody were purchased from Invitrogen. .. Cell culture HeLa cells were purchased from ATCC (CCL-2) and were cultured in DMEM (SH3008101; HyClone) supplemented with 10% FBS (Invitrogen) and 6 mM l -glutamine (Invitrogen). .. ATG5+/+ and ATG5−/− MEFs were donated by J. Brumell (University of Toronto, Toronto, Canada) and cultured in DMEM supplemented with 10% FBS and 6 mM l -glutamine.

    Article Title: High-content screen for modifiers of Niemann-Pick type C disease in patient cells
    Article Snippet: .. Fibroblasts were cultured in DMEM high glucose with 10% fetal bovine serum (Hyclone GE Life Sciences, SH30396, Logan, UT), Penicillin-Streptomycin (Corning 30-002-CI, Manassas, VA), and 1× GlutaMAX (Gibco/Thermo Fisher, 35050-061, Waltham, MA). .. Panobinostat was purchased from LC Labs (P-3703, Woburn, MA), and alexidine dihydrochloride from Cayman Chemical (13876, Ann Arbor, MI).

    Article Title: The Efficient Derivation of Trophoblast Cells from Porcine In Vitro Fertilized and Parthenogenetic Blastocysts and Culture with ROCK Inhibitor Y-27632
    Article Snippet: The bands were detected by chemiluminescence signals using ECL Western Blotting Substrate (Promega, W1001) and scanned to produce digital images. .. They are the high glucose DMEM supplement with 10% FBS (Hyclone, NWG0445) and 1mM glutamine and KF medium without bFGF. pTR cells that were mechanically cut under a microscope were cultured in the conditions that were mentioned above on 0.2% gelatin-coated dishes. .. Additionally, on day7 and day14, total RNA was isolated from cells using an RNeasy Mini kit (QIAGEN) according to the manufacturer’s protocol.

    Article Title: The molecular and cellular basis of exostosis formation in hereditary multiple exostoses
    Article Snippet: The PCR products were resolved by 1.5% agarose gel electrophoresis and visualized by ethidium bromide staining (Sigma-Aldrich). .. The rate of synthesis and GAG content in cultured cells were measured by incubating the cells in 2 ml medium supplemented with 10 μCi/ml of radioactive sodium sulphate [35 S Carrier free] (Amersham, Buckinghamshire, UK). ..

    Article Title: Network Organisation and the Dynamics of Tubules in the Endoplasmic Reticulum
    Article Snippet: .. METHODS VERO cells, an immortalised cell line derived from the kidney of an African green monkey, were cultured in Dulbecco’s Minimum Essential Media (DMEM) supplemented with 10% Fetal Bovine Serum (FBS, Hyclone, Logan, UT) at 37°C and 8% CO2. .. Fixed Imaging 48 hours prior to imaging, Vero cells were cultured on No 1.5 glass coverslips in a 12-well plate.

    Microscopy:

    Article Title: The Efficient Derivation of Trophoblast Cells from Porcine In Vitro Fertilized and Parthenogenetic Blastocysts and Culture with ROCK Inhibitor Y-27632
    Article Snippet: The bands were detected by chemiluminescence signals using ECL Western Blotting Substrate (Promega, W1001) and scanned to produce digital images. .. They are the high glucose DMEM supplement with 10% FBS (Hyclone, NWG0445) and 1mM glutamine and KF medium without bFGF. pTR cells that were mechanically cut under a microscope were cultured in the conditions that were mentioned above on 0.2% gelatin-coated dishes. .. Additionally, on day7 and day14, total RNA was isolated from cells using an RNeasy Mini kit (QIAGEN) according to the manufacturer’s protocol.

    Labeling:

    Article Title: An Acetylation Site in the Middle Domain of Hsp90 Regulates Chaperone Function
    Article Snippet: NIH3T3 cells stably expressing v-src have been described previously ( ; ). .. SkBr3 cells were labeled with 80 μCi[14 C] sodium acetate (Amersham) for 20 hours and lysed in buffer containing 50 mM Tris-HCl pH7.5, 100 mM NaCl, 1 mM EDTA (Biosource/Invtrogen), 1% NP40, 2 mM Na3 VO4 , 30 mM NaF, 10 mM sodium butyrate (Sigma), and protease inhibitors (Roche). .. Protein concentration was determined by BCA protein assay (Pierce), and Hsp90 was immunoprecipitated with anti-Hsp90 antibody (Affinity Bioreagents).

    Derivative Assay:

    Article Title: Network Organisation and the Dynamics of Tubules in the Endoplasmic Reticulum
    Article Snippet: .. METHODS VERO cells, an immortalised cell line derived from the kidney of an African green monkey, were cultured in Dulbecco’s Minimum Essential Media (DMEM) supplemented with 10% Fetal Bovine Serum (FBS, Hyclone, Logan, UT) at 37°C and 8% CO2. .. Fixed Imaging 48 hours prior to imaging, Vero cells were cultured on No 1.5 glass coverslips in a 12-well plate.

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  • 97
    GE Healthcare dulbecco s modified eagle s medium
    In vitro gene expression efficiency of pEGFP loaded in different gene carriers. Notes: ( A ) Lip2000; ( B ) TACS particle; and ( C ) TACS-HBC composite particle expression of pEGFP in HEK 293 cells after 72 hours. Expression efficiency was detected by fluorescence microscopy (images, top row) and flow cytometry (graphs, bottom row). Image and graph ( D ) represent the control, which was DMEM without any particles. Abbreviations: pEGFP, enhanced green fluorescent protein plasmids ; Lip2000, Lipofectamine ® 2000 transfection reagent; TACS, thiolated N-alkylated chitosan; HBC, hydroxybutyl chitosan; HEK 293T, human embryonic kidney cell line 293T; FL1, green fluorescence; LOG, logarithmic relative intensity of green fluorescence; DMEM, <t>Dulbecco’s</t> Modified Eagle Medium.
    Dulbecco S Modified Eagle S Medium, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dulbecco s modified eagle s medium/product/GE Healthcare
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dulbecco s modified eagle s medium - by Bioz Stars, 2021-03
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    In vitro gene expression efficiency of pEGFP loaded in different gene carriers. Notes: ( A ) Lip2000; ( B ) TACS particle; and ( C ) TACS-HBC composite particle expression of pEGFP in HEK 293 cells after 72 hours. Expression efficiency was detected by fluorescence microscopy (images, top row) and flow cytometry (graphs, bottom row). Image and graph ( D ) represent the control, which was DMEM without any particles. Abbreviations: pEGFP, enhanced green fluorescent protein plasmids ; Lip2000, Lipofectamine ® 2000 transfection reagent; TACS, thiolated N-alkylated chitosan; HBC, hydroxybutyl chitosan; HEK 293T, human embryonic kidney cell line 293T; FL1, green fluorescence; LOG, logarithmic relative intensity of green fluorescence; DMEM, Dulbecco’s Modified Eagle Medium.

    Journal: International Journal of Nanomedicine

    Article Title: The sustained-release behavior and in vitro and in vivo transfection of pEGFP-loaded core-shell-structured chitosan-based composite particles

    doi: 10.2147/IJN.S58104

    Figure Lengend Snippet: In vitro gene expression efficiency of pEGFP loaded in different gene carriers. Notes: ( A ) Lip2000; ( B ) TACS particle; and ( C ) TACS-HBC composite particle expression of pEGFP in HEK 293 cells after 72 hours. Expression efficiency was detected by fluorescence microscopy (images, top row) and flow cytometry (graphs, bottom row). Image and graph ( D ) represent the control, which was DMEM without any particles. Abbreviations: pEGFP, enhanced green fluorescent protein plasmids ; Lip2000, Lipofectamine ® 2000 transfection reagent; TACS, thiolated N-alkylated chitosan; HBC, hydroxybutyl chitosan; HEK 293T, human embryonic kidney cell line 293T; FL1, green fluorescence; LOG, logarithmic relative intensity of green fluorescence; DMEM, Dulbecco’s Modified Eagle Medium.

    Article Snippet: Cell culture A Hela cell line was cultured in a high-glucose solution of Dulbecco’s Modified Eagle’s Medium (DMEM; HyClone™, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with heat-inactivated 10% fetal bovine serum (FBS; Thermo Fisher Scientific, Waltham, MA, USA) and 1% penicillin-streptomycin (Harbin Pharmaceutical Group Holding Co., Ltd., Helongjiang, People’s Republic of China).

    Techniques: In Vitro, Expressing, Fluorescence, Microscopy, Flow Cytometry, Cytometry, Transfection, Modification

    MGF inhibits lactic acidosis. A : ECAR recorded in the same Seahorse Bioscience Metabolic Flux experiments as shown in Fig. 4 E . B : Lactate formed in the experiments shown in A was measured by a d -lactate assay kit. C : C2C12 myotubes were incubated overnight in glucose-free and sodium pyruvate–free DMEM containing l -glutamine and 2% horse serum, in the presence of various concentrations of MGF, and then incubated in media containing 25 mmol/L glucose in the presence of matching concentrations of MGF for 8 h. Media were collected, and lactate in the media was measured by a d -lactate assay kit (Eton Biosciences). These experiments were all conducted three to four times and each time with triplicates for each condition. Values are average ± SEM. ** P

    Journal: Diabetes

    Article Title: Mangiferin Stimulates Carbohydrate Oxidation and Protects Against Metabolic Disorders Induced by High-Fat Diets

    doi: 10.2337/db14-0006

    Figure Lengend Snippet: MGF inhibits lactic acidosis. A : ECAR recorded in the same Seahorse Bioscience Metabolic Flux experiments as shown in Fig. 4 E . B : Lactate formed in the experiments shown in A was measured by a d -lactate assay kit. C : C2C12 myotubes were incubated overnight in glucose-free and sodium pyruvate–free DMEM containing l -glutamine and 2% horse serum, in the presence of various concentrations of MGF, and then incubated in media containing 25 mmol/L glucose in the presence of matching concentrations of MGF for 8 h. Media were collected, and lactate in the media was measured by a d -lactate assay kit (Eton Biosciences). These experiments were all conducted three to four times and each time with triplicates for each condition. Values are average ± SEM. ** P

    Article Snippet: Cell Culture C2C12 myoblast cells were maintained in DMEM high glucose (HyClone Laboratories, Logan, UT), supplemented with 1% penicillin and streptomycin (Gibco, Carlsbad, CA) and 10% FBS.

    Techniques: Lactate Assay, Incubation

    MGF stimulates glucose oxidation. Palmitate ( A ) and glucose ( B ) oxidation was measured after a 12-h fast in soleus muscle isolated from mice fed with CD or HFD, with or without 0.5% MGF, for 18 weeks ( n = 6–7). C : [ 14 C]-labeled oleic acid oxidation in C2C12 myotubes pretreated or not with 200 μmol/L MGF for 16 h. Palmitate ( D ) and glucose ( E ) oxidation in C2C12 myotubes measured by Seahorse Bioscience Metabolic Flux analyzer. Myotubes differentiated from C2C12 cells in XF24 microplates were incubated overnight in glucose-free and sodium pyruvate–free DMEM, with or without 200 μmol/L MGF. OCR was recorded after injection of vehicle ( D ), BSA-conjugated palmitate ( D ), or d -glucose ( E ) into wells, with or without 200 μmol/L MGF. F : Intracellular ATP levels in C2C12 myotubes incubated overnight in glucose-free and sodium pyruvate–free DMEM in the presence of various concentrations of MGF and then incubated in media containing 25 mmol/L glucose with matching concentrations of MGF for 8 h. ATP was determined using the CellTiter-Glo Luminescent Cell Viability Assay kit according to the manufacturer’s instructions (Promega). In vitro experiments were conducted three to four times and each time with triplicates for each condition. Values are average ± SEM. * P

    Journal: Diabetes

    Article Title: Mangiferin Stimulates Carbohydrate Oxidation and Protects Against Metabolic Disorders Induced by High-Fat Diets

    doi: 10.2337/db14-0006

    Figure Lengend Snippet: MGF stimulates glucose oxidation. Palmitate ( A ) and glucose ( B ) oxidation was measured after a 12-h fast in soleus muscle isolated from mice fed with CD or HFD, with or without 0.5% MGF, for 18 weeks ( n = 6–7). C : [ 14 C]-labeled oleic acid oxidation in C2C12 myotubes pretreated or not with 200 μmol/L MGF for 16 h. Palmitate ( D ) and glucose ( E ) oxidation in C2C12 myotubes measured by Seahorse Bioscience Metabolic Flux analyzer. Myotubes differentiated from C2C12 cells in XF24 microplates were incubated overnight in glucose-free and sodium pyruvate–free DMEM, with or without 200 μmol/L MGF. OCR was recorded after injection of vehicle ( D ), BSA-conjugated palmitate ( D ), or d -glucose ( E ) into wells, with or without 200 μmol/L MGF. F : Intracellular ATP levels in C2C12 myotubes incubated overnight in glucose-free and sodium pyruvate–free DMEM in the presence of various concentrations of MGF and then incubated in media containing 25 mmol/L glucose with matching concentrations of MGF for 8 h. ATP was determined using the CellTiter-Glo Luminescent Cell Viability Assay kit according to the manufacturer’s instructions (Promega). In vitro experiments were conducted three to four times and each time with triplicates for each condition. Values are average ± SEM. * P

    Article Snippet: Cell Culture C2C12 myoblast cells were maintained in DMEM high glucose (HyClone Laboratories, Logan, UT), supplemented with 1% penicillin and streptomycin (Gibco, Carlsbad, CA) and 10% FBS.

    Techniques: Isolation, Mouse Assay, Labeling, Incubation, Injection, Cell Viability Assay, In Vitro

    MGF enhances pyruvate oxidation by activating PDH. A : Pyruvate oxidation in myotubes differentiated from C2C12 cells measured by Seahorse Bioscience Metabolic Flux analysis. Myotubes differentiated from C2C12 cells in XF24 microplates were incubated overnight in glucose-free and sodium pyruvate–free DMEM, with and without 200 μmol/L MGF. OCR was recorded after injection of sodium pyruvate into wells, with or without 200 μmol/L MGF. B : PDH activities measured as the difference between absorbances at 500 nm and 750 nm at 25°C in mitochondrial protein from C2C12 myotubes treated with various concentrations of MGF for 24 h or 15 min or with 5 mmol/L DCA or FP + 200 μmol/L MGF for 15 min. C : Initial rates of PDH-catalyzed pyruvate oxidation measured as the difference between absorbances at 500 nm and 750 nm in mitochondrial protein from C2C12 myotubes treated with various concentrations of MGF for 24 h or 15 min. The unit of activity was determined by using extinction coefficient of reduced p-iodonitrotetrazolium violet (12.4 mmol ⋅ L −1 ⋅ cm −1 ). D : Initial rates of PDH catalyzed [ 14 C]-labeled pyruvate oxidation measured as the production of 14 CO 2 by mitochondrial protein from C2C12 myotubes treated with various concentrations of MGF for 15 min. E : Western blots of proteins extracted from C2C12 myotubes treated with 200 or 1,000 μmol/L MGF for 15 min and 24 h. Membrane was blotted with PDH (1:250 dilution; Cell Signaling), p-PDH (1:2,000; Abcam), PDK4 (1:500; laboratory of Dr. Robert Harris at Indiana University School of Medicine), or α-tubulin (1:2,000; Sigma-Aldrich) at 4°C overnight. F : Quantitative analysis of Western blots shown in E . G : Western blots of proteins extracted from C2C12 myotubes treated with 200 or 1,000 μmol/L MGF or 5 mmol/L DCA for 15 min. H : Quantitative analysis of Western blots shown in G . Values are average ± SEM. * P

    Journal: Diabetes

    Article Title: Mangiferin Stimulates Carbohydrate Oxidation and Protects Against Metabolic Disorders Induced by High-Fat Diets

    doi: 10.2337/db14-0006

    Figure Lengend Snippet: MGF enhances pyruvate oxidation by activating PDH. A : Pyruvate oxidation in myotubes differentiated from C2C12 cells measured by Seahorse Bioscience Metabolic Flux analysis. Myotubes differentiated from C2C12 cells in XF24 microplates were incubated overnight in glucose-free and sodium pyruvate–free DMEM, with and without 200 μmol/L MGF. OCR was recorded after injection of sodium pyruvate into wells, with or without 200 μmol/L MGF. B : PDH activities measured as the difference between absorbances at 500 nm and 750 nm at 25°C in mitochondrial protein from C2C12 myotubes treated with various concentrations of MGF for 24 h or 15 min or with 5 mmol/L DCA or FP + 200 μmol/L MGF for 15 min. C : Initial rates of PDH-catalyzed pyruvate oxidation measured as the difference between absorbances at 500 nm and 750 nm in mitochondrial protein from C2C12 myotubes treated with various concentrations of MGF for 24 h or 15 min. The unit of activity was determined by using extinction coefficient of reduced p-iodonitrotetrazolium violet (12.4 mmol ⋅ L −1 ⋅ cm −1 ). D : Initial rates of PDH catalyzed [ 14 C]-labeled pyruvate oxidation measured as the production of 14 CO 2 by mitochondrial protein from C2C12 myotubes treated with various concentrations of MGF for 15 min. E : Western blots of proteins extracted from C2C12 myotubes treated with 200 or 1,000 μmol/L MGF for 15 min and 24 h. Membrane was blotted with PDH (1:250 dilution; Cell Signaling), p-PDH (1:2,000; Abcam), PDK4 (1:500; laboratory of Dr. Robert Harris at Indiana University School of Medicine), or α-tubulin (1:2,000; Sigma-Aldrich) at 4°C overnight. F : Quantitative analysis of Western blots shown in E . G : Western blots of proteins extracted from C2C12 myotubes treated with 200 or 1,000 μmol/L MGF or 5 mmol/L DCA for 15 min. H : Quantitative analysis of Western blots shown in G . Values are average ± SEM. * P

    Article Snippet: Cell Culture C2C12 myoblast cells were maintained in DMEM high glucose (HyClone Laboratories, Logan, UT), supplemented with 1% penicillin and streptomycin (Gibco, Carlsbad, CA) and 10% FBS.

    Techniques: Incubation, Injection, Activity Assay, Labeling, Western Blot

    Effects of Thd on HG-induced MC viability by MTT assay. NG-cultured MCs were grown in DMEM containing 5.6 mmol/l D-glucose, whilst HG-cultured MCs were grown in Dulbecco's modified Eagle's medium containing 30 mmol/l D-glucose and following treatment with various concentrations (0, 10, 50, 100 and 200 µg/ml) of Thd for 12, 24 or 48 h the MTT assay was used to measure cell viability. Data are presented as the mean ± standard error of the mean (n=5). # P

    Journal: Experimental and Therapeutic Medicine

    Article Title: Thalidomide decreases high glucose-induced extracellular matrix protein synthesis in mesangial cells via the AMPK pathway

    doi: 10.3892/etm.2018.6995

    Figure Lengend Snippet: Effects of Thd on HG-induced MC viability by MTT assay. NG-cultured MCs were grown in DMEM containing 5.6 mmol/l D-glucose, whilst HG-cultured MCs were grown in Dulbecco's modified Eagle's medium containing 30 mmol/l D-glucose and following treatment with various concentrations (0, 10, 50, 100 and 200 µg/ml) of Thd for 12, 24 or 48 h the MTT assay was used to measure cell viability. Data are presented as the mean ± standard error of the mean (n=5). # P

    Article Snippet: Cells were cultured in Dulbecco's modified Eagle's media (DMEM; HyClone; GE Healthcare Life Sciences, Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS; HyClone; GE Healthcare Life Sciences) and maintained at 37°C in a 5% CO2 -humidified incubator.

    Techniques: MTT Assay, Cell Culture, Modification

    Characteristics of PDMCs. (A) Representative data of reverse transcription- polymerase chain reaction analysis of rat (n=10) and human (n=4) PDMCs for stemness- and trophoblast-related transcripts. (B) Differentiation of rat PDMCs into multiple lineages. Adipogenic differentiation is demonstrated by the accumulation of intracellular lipid droplets stained by Oil Red O. Osteogenic differentiation is demonstrated by the deposition of extracellular calcium visualized through Alizarin Red S staining. Negative control cells were grown in Dulbecco's modified Eagle medium supplemented with 10% fetal bovine serum; no Alizarin Red S or Oil Red O staining was observed. Scale bar, 50 µm. (C) Representative data of fluorescence in situ hybridization analysis of rat (n=3) and human (n=3) PDMCs at the 3rd passage for X (orange)/Y (green) chromosome. Scale bar, 10 µm. PDMCs, placenta-derived multipotent cells; CDX2, caudal type homeobox 2; ID2, inhibitor of DNA binding 2; POU5F1, POU class 5 homeobox 1; Tpbpa, trophoblast specific protein α; Prl3b1, prolactin family 3, subfamily b, member 1; EOMES, eomesodermin; CGB, choriogonadotropin subunit-β; ERVW-1, endogenous retrovirus group W envelope member 1.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Placenta-derived multipotent cells have no effect on the size and number of DMH-induced colon tumors in rats

    doi: 10.3892/etm.2017.4792

    Figure Lengend Snippet: Characteristics of PDMCs. (A) Representative data of reverse transcription- polymerase chain reaction analysis of rat (n=10) and human (n=4) PDMCs for stemness- and trophoblast-related transcripts. (B) Differentiation of rat PDMCs into multiple lineages. Adipogenic differentiation is demonstrated by the accumulation of intracellular lipid droplets stained by Oil Red O. Osteogenic differentiation is demonstrated by the deposition of extracellular calcium visualized through Alizarin Red S staining. Negative control cells were grown in Dulbecco's modified Eagle medium supplemented with 10% fetal bovine serum; no Alizarin Red S or Oil Red O staining was observed. Scale bar, 50 µm. (C) Representative data of fluorescence in situ hybridization analysis of rat (n=3) and human (n=3) PDMCs at the 3rd passage for X (orange)/Y (green) chromosome. Scale bar, 10 µm. PDMCs, placenta-derived multipotent cells; CDX2, caudal type homeobox 2; ID2, inhibitor of DNA binding 2; POU5F1, POU class 5 homeobox 1; Tpbpa, trophoblast specific protein α; Prl3b1, prolactin family 3, subfamily b, member 1; EOMES, eomesodermin; CGB, choriogonadotropin subunit-β; ERVW-1, endogenous retrovirus group W envelope member 1.

    Article Snippet: To allow cell migration from the tissue onto the culture plate, human tissue fragments were covered with high-glucose Dulbecco's modified Eagle medium (DMEM; Hyclone; GE Healthcare Life Sciences) supplemented with 10% fetal bovine serum (FBS; Hyclone; GE Healthcare Life Sciences), and rat tissue was covered with high-glucose DMEM supplemented with 7% FBS and 3% rat serum (BioWest, Nuaillé, France).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Staining, Negative Control, Modification, Fluorescence, In Situ Hybridization, Derivative Assay, Binding Assay